CN109504388A - A kind of preparation method of moss type concave convex rod based high molecular sand fixation material - Google Patents
A kind of preparation method of moss type concave convex rod based high molecular sand fixation material Download PDFInfo
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- CN109504388A CN109504388A CN201811609574.3A CN201811609574A CN109504388A CN 109504388 A CN109504388 A CN 109504388A CN 201811609574 A CN201811609574 A CN 201811609574A CN 109504388 A CN109504388 A CN 109504388A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/18—Prepolymers; Macromolecular compounds
- C09K17/20—Vinyl polymers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
- A01G24/35—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds containing water-absorbing polymers
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2105/00—Erosion prevention
Abstract
The present invention provides a kind of preparation method of moss type concave convex rod based high molecular sand fixation material, includes the following steps: the pretreatment of (1) desert moss crust and expands numerous;(2) preparation of the pretreatment of concave convex rod and concave convex rod base high water-absorption resin;(3) preparation of moss type concave convex rod based high molecular sand fixation material.This autotrophic sand-consolidating agent with self-reproduction ability and bioactivity of the invention, while strengthening conventional polymer sand fixation material performance, suitable nutritional condition and water condition are provided for the amplification of moss crust, surface soil stability can be enhanced, promote moss crust development, achievees the purpose that administer desertification of land.
Description
Technical field
The invention belongs to technical fields of defending and controlling sand, and in particular to a kind of moss type concave convex rod based high molecular sand fixation material
Preparation method.
Background technique
Desertification is one of the main ecological problem that the whole world faces, and brings huge loss to economic results in society,
Seriously threaten the existence and sustainable development of the mankind.The Practice Development in 20th century is it has been proved that development of resources and society, economy
Development must all pay attention to the synchronized construction with ecological environment, World Ecosystem environmental project construction project is by desert chemoprevention at present
It controls and is classified as one of main construction project.
Currently, more common sand fixing technology mainly has engineering (or physics) to fix the sand, sand consolidation with biologic, chemical sand-fixing and synthesis
It fixes the sand, but the research for preparing series of biologic type composite high-molecular sand fixation material is near barren.And that studies at present most of fixes the sand
Material can all generate secondary pollution, if waterglass class sand fixation material will cause alkali pollution, inhibit plant growth, only part high score
Sub- sand fixation material, it is easily decomposes after the intensive ultraviolet sunlight irradiation by desert area.China's concave convex rod storage capacity accounts for generation
95% or more of boundary's total storage, the concave convex rod distant view reserves that wherein coming from L inze County is verified are about 1,000,000,000 tons, account for world's distant view
85% or more of reserves has been widely used in multiple fields due to the special physics of attapulgite and chemical property, but will system
Standby composite high-molecular sand fixation material compounds according to a certain ratio, under the conditions of serial environment with artificial bio-membrane's skinning, if
It can prepare without secondary pollution to environment, with more the sand fixation material of trophism and fixing the sand property, there is no exploration.
Summary of the invention
In order to solve the problems in the existing technology, the present invention provides a kind of moss type concave convex rod based high moleculars to fix the sand
The preparation method of material.
The present invention provides a kind of preparation method of moss type concave convex rod based high molecular sand fixation material, includes the following steps:
(1) pretreatment and expansion of desert moss crust are numerous:
A, the pretreatment of desert moss crust: desert moss crust is cleaned and sterilized;
B, the expansion of artificial culture moss crust is numerous:
The pretreated desert moss crust of step a is inoculated into culture medium and is cultivated;
The culture medium includes Hogland culture medium and plant growth regulator, and the plant growth regulator is 0.4-
The 2,4-D of the 6-B-A or 0.4-0.6mg/L of 0.6mg/L;
The culture is temperature is 30 DEG C, intensity of illumination is 120 μ E/ (m2·s1), light dark period be 12h:12h
It is cultivated in environment;
(2) preparation of the pretreatment of concave convex rod and concave convex rod base high water-absorption resin:
A, removal of impurities the pretreatment of concave convex rod: is cleaned after attapulgite is ground;
B, pretreated concave convex rod is subjected to heat modification;
C, the preparation of concave convex rod base high water-absorption resin:
By acrylic acid be added sodium hydroxide solution in, under ice-water bath in and, degree of neutralization 80%, be added acrylamide,
Heat modification concave convex rod is added, polymerized monomer is obtained;Crosslinking agent and initiator are added in monomer, stirred evenly, in certain item
Fully reacting under part obtains concave convex rod base high water-absorption resin;
The crosslinking agent is N, N '-methylene-bisacrylamide;
The initiator is the potassium peroxydisulfate and sodium hydrogensulfite that molar ratio is 1:1;
(3) preparation of moss type concave convex rod based high molecular sand fixation material:
The concave convex rod base high water-absorption resin of the desert moss crust of step (1) preparation and step (2) preparation is mixed equal
It is even, obtain moss type concave convex rod based high molecular sand fixation material.
Preferably, the heat modification is the heat modification carried out under conditions of 400 DEG C 1 hour in step (2).
Preferably, the addition mass percent of the heat modification concave convex rod is 10% in step (2).
Preferably, the dosage of the crosslinking agent is the 0.05% of polymerized monomer weight in step (2);The initiator
Dosage be polymerized monomer weight 0.6%.
Preferably, the fully reacting under certain condition is to react 60 under 800W microwave power in step (2)
Second.
Preferably, in step (2), the fully reacting under certain condition is the ultrasonic power at 80 DEG C of bath temperature
To be reacted 120 minutes when 200W.
Preferably, in step (2), the acrylic acid, acrylic amine amount ratio be 15ml:4g.
Preferably, the mass ratio of desert moss crust and concave convex rod base high water-absorption resin is 1:1 in step (3).
The present invention provides a kind of moss type concave convex rod based high molecular sand fixation material, is to be prepared with the aforedescribed process
's.
The present invention provides application of the moss type concave convex rod based high molecular sand fixation material in fixing the sand.
Preferably, described fix the sand are as follows: be sprayed at sand fixation material in the culture dish for being covered with sterile sand, kept when initial
Shamian Island is wet, is cultivated, an every 1-3 days moisture of supplement and Hoglang nutrient solution, the Hoglang nutrient solution and distillation
The volume ratio of water is 1:1.
The beneficial effects of the present invention are: this autotrophic with self-reproduction ability and bioactivity of the invention fixes the sand
Agent, while strengthening conventional polymer sand fixation material performance, for moss crust amplification provide suitable nutritional condition and
Surface soil stability can be enhanced in water condition, promotes moss crust development, achievees the purpose that administer desertification of land.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings:
Fig. 1 is response of the moss Determination of Chlorophyll a content at any time to culture substrate variation.
Fig. 2 is the mda content response to culture substrate variation at any time in moss.
Fig. 3 is the soluble sugar content response to culture substrate variation at any time in moss.
Fig. 4 is the soluble protein content response to culture substrate variation at any time in moss.
Fig. 5 is the response that moss Determination of Chlorophyll a content at any time changes sugar source type and concentration.
Fig. 6 is the response that mda content at any time changes sugar source type and concentration in moss.
Fig. 7 is the response that soluble protein content at any time changes sugar source type and concentration in moss.
Fig. 8 is the response that moss Determination of Chlorophyll a content at any time changes plant growth regulator type and concentration.
The response that mda content at any time changes plant growth regulator type and concentration in Fig. 9 moss.
The response that soluble protein content at any time changes plant growth regulator type and concentration in Figure 10 moss.
Figure 11 is the response that soluble sugar content at any time changes plant growth regulator type and concentration in moss.
Concave convex rod base high water-absorption resin is in distilled water (pH6.22 when Figure 12 is the mass fraction of different heat modification concave convex rods
Left and right) in different time water absorbent rate.
Concave convex rod base high water-absorption resin is molten in 0.9% sodium chloride when Figure 13 is the mass fraction of different heat modification concave convex rods
The water absorbent rate of different time in liquid.
Figure 14 be concave convex rod base high water-absorption resin maximum water absorbent rate with heat modification concave convex rod mass fraction degree and
The variation tendency of solution ph.
Figure 15 is that different ultrasonic power interferes lower APT10 concave convex rod base high water-absorption resin at distilled water (pH6.22 or so)
The water absorbent rate of middle different time.
Figure 16 is that different ultrasonic power interferes lower APT10 concave convex rod base high water-absorption resin in 0.9% sodium chloride solution
The absorbency of different time.
Figure 17 is that moss Determination of Chlorophyll a content at any time fixes the sand material to the moss type concave convex rod based high moleculars of different compounding ratios
The response of material.
Figure 18 is the moss crust of moss type concave convex rod based high molecular sand fixation material culture under different moisture content and nutritional condition
Determination of Chlorophyll a content with/time changing rule.
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified city
It sells.
Embodiment 1
One, the preparation of moss type concave convex rod based high molecular sand fixation material
(1) pretreatment to experiment raw material:
A, desert moss crust: material picks up from Tengger desert south Shapotou Area, ningxia Hui Autonomous Region, China (37 ° of 26 ' 46.9 " E of N
104 ° 50 ' 11.2 ") natural moss type desert biological breadcrust, utilize pvc pipe (internal diameter 10cm, high 5cm) grab sample, sampling
Depth 0-2cm acquires 36 moss type (being true moss) desert biological breadcrust samples altogether, and sample collection is in the polybag of sealing
In.After sample is taken back laboratory, the vigorous moss plant of growing way is picked out, under ground portion is removed with blade, then originally
It is underwater to rinse plant until being mingled with again without the grains of sand between the moss strain cauline leaf observed under anatomical lens, finally stem apex is taken under anatomical lens
Portion about 5mm, taken stem apex is placed in the vessel equipped with 0.5% liquor natrii hypochloritis and impregnates 30s, with a large amount of nothings after sanitized
Bacterium water is rinsed, and is then cultivated moss sample inoculation in artificial climate incubator into 4 kinds of culture mediums.
B, concave convex rod: Gansu Province, slab bridge town, Linze (39 ° 15 ' of N 100 ° 16 ' of E, height above sea level 1465m) mine point acquisition mine is picked up from
Red natural Attapulgite Clay Minerals are crushed, using careful grinding through crusher, concave convex rod are then crossed 200 targets standard by sample
Sieve, obtains attapulgite raw ore powder.It is washed with distilled water concave convex rod, concave convex rod particle is stirred well to and all disperses, it is static
30min removes suspension upper layer impurity and bottom black precipitate.It washs repeatedly, until bottom black precipitate is all removed.
Simultaneously 100 DEG C of vacuum drying concave convex rods are separated, are sieved with 100 mesh sieve after grinding.
The preparation process of heat modification attapulgite is as follows: weigh the attapulgite of certain mass under conditions of 400 DEG C into
1 hour heat modification of row, it is to be modified after attapulgite it is cooling after sieve with 100 mesh sieve, be put into spare in drier.
(2) it compounds:
A, the expansion of artificial culture moss crust is numerous
Temperature be 30 DEG C, intensity of illumination be 120 μ E/ (m2·s1), light dark period be 12h:12h artificial climate train
It supports and cultivates moss plant in case, choose tetra- kinds of culture mediums (table 1.1) of Beneck, Part, BG11, Hogland, after culture 10 days,
Continuous five days measurement physical signs (content of chlorophyll a, MDA, soluble protein and soluble sugar).
Add 10 respectively in the optimal medium of moss, 20, the sucrose of 40g/L and glucose (amount to 6 kinds of culture items
Part), amount to culture 60 days, regularly replace nutrient solution according to the actual situation, measure within every 10 days its physical signs (chlorophyll a, MDA,
The content of soluble protein).
Add 0.5 respectively in the optimal medium of moss, 1,2, the 4-D of 2mg/L and 6-B-A (amount to 6 kinds of culture items
Part), amount to culture 60 days, regularly replace nutrient solution according to the actual situation, measure within every 10 days its physical signs (chlorophyll a, MDA,
The content of soluble protein and soluble sugar).
The culture medium main ingredient of 1 moss type desert biological breadcrust of table
B, the preparation of concave convex rod base high water-absorption resin
It is reacted according to one of following two methods, the purposes of following two methods is only for ensuring to shorten the reaction time
And the performance for the concave convex rod base high water-absorption resin for reacting fully reacting, therefore following two methods being used to be prepared is identical:
First method:
The NaOH for weighing 24g is dissolved in beaker with 40mL distilled water.Another measure 15mL acrylic acid (AA) is slowly added to hydrogen
In sodium hydroxide solution, under ice-water bath in and, degree of neutralization 80%.The acrylamide (AM) for adding 4.0g, be then added with
Super absorbent resin meter different quality containing 0,3%, 5%, 8%, 10%, 15% (be denoted as respectively ATP0, ATP3, ATP5,
ATP8, ATP10, ATP15) heat modification concave convex rod sample, stir evenly, obtain polymerized monomer.Take crosslinking agent N, N '-methylene
Bisacrylamide dosage is 0.05% (mass ratio accounts for the mass fraction of polymerized monomer), initiator potassium persulfate and bisulfite
Sodium (molar ratio that potassium peroxydisulfate and sodium hydrogensulfite press 1:1) dosage is that 0.6% (mass ratio accounts for the quality point of polymerized monomer
Number), it is dissolved in 10mL distilled water, adds it in polymerized monomer, stir evenly.
Beaker is transferred in microwave reactor, is reacted 60 seconds under certain microwave power (800W).A period of time is extremely
After complete reaction, thick product is obtained, product is shredded, is washed with ethanol water.Later, it is put into baking oven in 90 DEG C
It dry, pulverize, collect the product of 100 mesh up to sample.
Second method:
The method of concave convex rod base high water-absorption resin is prepared under ultrasound interference are as follows:
The NaOH for weighing 24g is dissolved in beaker with 40mL distilled water.Another measure 15mL acrylic acid (AA) is slowly added to hydrogen
In sodium hydroxide solution, under ice-water bath in and, degree of neutralization 80%.The acrylamide (AM) for adding 4.0g, be then added with
The heat modification concave convex rod sample of super absorbent resin meter mass fraction 10%, stirs evenly, obtains polymerized monomer.Crosslinking agent N is taken,
N '-methylene-bisacrylamide dosage be 0.05% (mass ratio accounts for the mass fraction of polymerized monomer), initiator potassium persulfate and
Sodium hydrogensulfite (molar ratio that potassium peroxydisulfate and sodium hydrogensulfite press 1:1) dosage is that 0.6% (mass ratio accounts for polymerized monomer
Mass fraction), it is dissolved in 10mL distilled water, adds it in polymerized monomer, stir evenly.
Open ultrasound, be set separately power be 200,300,400,500W, set bath temperature as 80 DEG C, reached to temperature
When to designated value (80 DEG C), beaker is put into ultrasonic cleaning machine and is reacted, a period of time (120 minutes) after to fully reacting,
Product is shredded, is washed with ethanol water.Later, being put into baking oven dry, pulverize in 90 DEG C, and the product for collecting 100 mesh is
Obtain sample.
C, the compounding of moss type concave convex rod based high molecular sand fixation material
By artificial room cultivate moss air-dry under field conditions (factors), in different quality ratio (3:1,2:1,1:1,1:0,
1:2,1:3) compounding, i.e., moss is uniformly mixed with concave convex rod base high water-absorption resin, it is high that moss type concave convex rod base is prepared
Molecule sand fixation material.
Prepare sand-fixation method specifically:
Moss quality is certain, then with 400g/m2Sprinkling ratio by mixture (the moss type concave convex rod base high score of preparation
Sub- sand fixation material) it is sprayed in the culture dish of diameter 15cm, the sterile sand of about 1cm thickness, experimentation are covered in culture dish
In every the primary moisture content of supplement of 12 hours water sprays, until Shamian Island is wet, be placed in temperature be 30 DEG C, intensity of illumination be
120μE/(m2·s1), cultivate 80 days in the artificial climate incubator that light dark period is 12h:12h, sprinkling in every 7 days in right amount (50~
60ml) nutrient solution, the Chlorophyll-a Content of the moss crust of measurement in every 20 days, culture are dried at 30 DEG C after 80 days, measure tongue fur
Skinning thickness, skinning pH value and the skinning compression strength of moss skinning.
Wherein, the formula of nutrient solution are as follows: according to Hoglang culture medium in table 1 each constituent precise and carry out
Dissolution is configured to required nutrient solution.
Two, the performance study of moss type concave convex rod based high molecular sand fixation material
(1) moss physiological index determining method
A, the measurement of Chlorophyll-a Content: weighing fresh sample 0.2g, and micro quartz sand, calcium carbonate and 2mL 95% is added
Ethyl alcohol after be ground to tissue turned pale, be settled to 5mL with 95% ethyl alcohol, be centrifuged 10min, revolving speed 6000rpm, centrifugation twice, tongue fur
Muscoid form desert biological breadcrust is settled to 25mL with 95% ethyl alcohol, with the absorbance value of spectrophotometric determination 665 and 649nm, root
Chlorophyll-a Content is calculated according to formula 1.1.
Chlorophyll=13.95A665-6.88A649 (1.1)
B, the measurement of malonaldehyde (MDA) content: taking fresh sample 0.5g that the trichloroacetic acid and a small amount of quartz sand of 5mL 5% is added,
It is ground to homogenate, is centrifuged 10min, revolving speed 8000rpm.Supernatant 2mL is taken, the thiobarbituricacidα- for adding 2mL 0.67% is molten
Liquid.Mixed liquor heats 30min in boiling water bath, is centrifuged after being cooled to room temperature.Supernatant is taken to survey suction at 532,600 and 450nm
Shading value is calculated the content of malonaldehyde by formula 1.2.
MDA(μmol.L-1(the OD of)=6.45532-OD600)-0.56OD450 (1.2)
C, the measurement of content of soluble protein: taking the fresh sample of 0.3g, and 5mL distilled water is added to grind, and is centrifuged 10min, revolving speed
4000rpm, take supernatant 1.0mL be added tool plug test tube in, then plus 5mL Coomassie brilliant G-250 solution, stood after being sufficiently mixed
2min surveys absorbance value at 595nm, makees standard curve with 0-100 μ gm/L bovine serum albumin(BSA).
D, the measurement of soluble sugar content: taking the fresh sample of 0.2g, is put into band plug scale test tube, 10mL distilled water is added,
30min is heated in boiling water, is extracted 2 times, is placed in 25mL volumetric flask after extracting solution filtering, rinses constant volume after residue repeatedly.It takes
Then 0.5mL anthrone ethyl acetate and the 5mL concentrated sulfuric acid are added into test tube, sufficiently vibrates, test tube is put into for 0.5mL extracting solution
In boiling water bath, 1min is kept the temperature, is cooled to room temperature, absorbance value is surveyed in 630nm, with 0-100 μ gmL-1Sucrose makees standard curve.
(2) moss crust physical index measuring method
The color and configuration of surface for the moss type skinning that ocular estimate observation generates;With the pH value of pH meter measurement moss crust
(ratio of soil and water is 1:5);Use the thickness of vernier caliper measurement moss crust;Skinning cortex to be measured taking-up is placed on
On the sandy soils of 35mm thickness, intensity test is carried out using pull and push dynamometer, it is cracked for failure judgment with skinning to be measured, with
Peak stress calculates its compression strength.
(3) concave convex rod base high water-absorption resin water-absorbing-retaining performance index determining method
A, concave convex rod base high water-absorption resin water absorbent rate measures: weigh the dry sample of 0.5g (6 groups, wherein control group
CK is ATP0), every group of 3 repetition) it is separately added into the beaker equipped with 500mL distilled water, it is stood at room temperature to it and is sufficiently absorbed water
Reach saturation, with 100 mesh screens by after water suction resin filter, until it is anhydrous drip subject to.Weigh up the quality of water absorbent gel.It presses
Formula calculates water absorbent rate:
Q1=(M2-M1)/M1 (2.3)
In formula: Q1For liquid absorbency rate, g/g;M1To be swollen preceding resin sample quality, g;M2For swelling after resin sample quality,
g。
B, concave convex rod base high water-absorption resin Smoking indexes measure: accurately weigh the dry sample of 0.5g (6 groups, wherein
Control group CK is ATP0, every group of 3 repetition), it puts in the beaker equipped with 500mL concentration for 0.9% NaCl solution, it is quiet at room temperature
Set to it after sufficiently absorbing water and reaching saturation, with the screen to filtrate of 100 mesh, until it is anhydrous drip subject to.Weigh up the quality of water absorbent gel.
Smoking indexes are calculated as follows:
Q2=(M2-M1)/M1 (2.4)
In formula: Q2For liquid absorbency rate, g/g;M1To be swollen preceding resin sample quality, g;M2For swelling after resin sample quality,
g。
C, concave convex rod base high water-absorption resin water retention measures.Weigh 0.5g inhale water saturated sample (6 groups, wherein compareing
Group CK is purely a modified concave convex rod and pure high molecular material, every group of 3 repetition) record initial weight, it is placed in the training of 24 φ 150mm
It supports in ware, is put into 50 DEG C of thermostatic drying chambers, according to taking-up sample in certain time interval (every 30min) and weigh.Water conservation
Rate φ is calculated according to the following formula
φ=W2/W1× 100% (2.5)
In formula: φ is water retention, %;W2: the gel quality after dehydration, g;W1: the gel quality after water suction saturation, g.
D, concave convex rod base high water-absorption resin water absorbent rate measures under condition of different pH: weighing 0.5g sample and is placed in 1000mL
In beaker, 500mL deionized water is added, adjusts solution initial pH value with the HCl or NaOH solution of 0.1M, is set as 5-9 totally 5
Processing stirs at room temperature to material and reaches swelling equilibrium, investigates influence of the pH value variation to water absorbent rate.
(4) performance test that fixes the sand of moss type concave convex rod based high molecular sand fixation material
Moss type concave convex rod base sand fixation material is prepared by optimum compound proportion (1:1), with 400g/m2Sprinkling ratio sprayed
It is sprinkled upon in the culture dish of diameter 15cm, the sterile sand of about 1cm thickness is covered in culture dish, keep Shamian Island wet when initial, be placed in
Temperature be 30 DEG C, intensity of illumination be 120 μ E/ (m2·s1), light dark period be 12h:12h artificial climate incubator in cultivate
80 days, by moisture content of the water condition and nutritional condition gradient of setting every 1,3,6 day supplement, (simulation was wet, semi-moist half-dried
Drought, drought environment), water is indefinite, but the surface wettability of the moss crust under the conditions of making every kind, there is minute quantity ponding.Each
Water gradient divides into three vegetative gradients, i.e., nutrient solution and distilled water volume are than the nutrient solution that is configured to for 1:0,1:1,0:1.Often
The physical signs (chlorophyll a) of measurement in 20 days, culture 80 days after dried at 30 DEG C, measure moss crust skinning thickness,
Skinning pH value and skinning compression strength.
Wherein, the formula of nutrient solution are as follows: according to Hoglang culture medium in table 1 each constituent precise and carry out
Dissolution is configured to required nutrient solution, and then nutrient solution and distilled water volume ratio are that 1:0,1:1,0:1 are configured to three vegetative gradients
Nutrient solution.
Three, experiment conclusion
(1), the expansion of artificial culture moss crust is numerous
A, Fig. 1-4 is shown in using the physical signs of moss crust when different culture medium.
Fig. 1 is response of the moss Determination of Chlorophyll a content at any time to culture substrate variation.
Note: capitalization indicates that, there are significant difference (p < 0.05) between incubation time, lowercase indicates the same time
There are significant differences between culture substrate.
As seen from Figure 1: totality is presented in the Chlorophyll-a Content of moss in tri- kinds of culture mediums of Beneck, Part, BG11
Downward trend, and the Chlorophyll-a Content of Hogland culture moss was decreased significantly fluctuation at the 12nd day, from the 13rd day
Overall is in significant ascendant trend.
Fig. 2 is the mda content response to culture substrate variation at any time in moss.
Note: capitalization indicates that, there are significant difference (p < 0.05) between incubation time, lowercase indicates the same time
There are significant differences between culture substrate.
As seen from Figure 2: the mda content of tetra- kinds of culture medium culture mosses of Beneck, BG11, Hogland has pole
The content fluctuation of significant changes, Part is then smaller, and mda content maintains essentially in 6-7nmol/g;Moss in BG11 culture medium
Mda content initial maintenance high horizontal upper --- 13-12nmol/g then has extremely significant decline, and final stablize exists
6nmol/g or so;The mda content of moss maintains 4nmol/g or so always in Hogland culture medium, substantially less than other
Three kinds of culture mediums.
Fig. 3 is the soluble sugar content response to culture substrate variation at any time in moss.
Note: capitalization indicates that, there are significant difference (p < 0.05) between incubation time, lowercase indicates the same time
There are significant differences between culture substrate.
As seen from Figure 3: the soluble sugar content of moss in tetra- kinds of culture mediums of Beneck, Part, BG11, Hogland
It is generally on a declining curve, but in Hogland the soluble sugar content highly significant of moss be lower than other culture mediums.
Fig. 4 is the soluble protein content response to culture substrate variation at any time in moss.
Note: capitalization indicates that, there are significant difference (p < 0.05) between incubation time, lowercase indicates the same time
There are significant differences between culture substrate.
As seen from Figure 4: the soluble protein content of moss maintains always without significant changes in Beneck culture medium
0.22mg/g or so;The soluble protein content initial value of moss is high in Part, is rising the wave declined again after decline
It tends towards stability after dynamic;The soluble protein content of moss is first in significant ascendant trend in BG11, and soluble protein content is up to
0.586 ± 0.036mg/g is then in significant downward trend, but soluble protein content variable quantity is in diminution trend;Hogland
The soluble protein content of moss is totally in rising trend in culture medium, but declines afterwards in first rising at any time, then what is risen become
Gesture.
To sum up, Hogland culture medium is when optimum landscape in Shapotou area moss indoors artificial is cultivated in four kinds of culture mediums
Culture substrate.
B, the physical signs of moss crust is shown in Fig. 5-7 when adding different sugar sources in optimal medium.
Fig. 5 is the response that moss Determination of Chlorophyll a content at any time changes sugar source type and concentration.
Fig. 6 is the response that mda content at any time changes sugar source type and concentration in moss.
Fig. 7 is the response that soluble protein content at any time changes sugar source type and concentration in moss.
The extremely significant content for influencing moss Determination of Chlorophyll a, malonaldehyde of the type and concentration of sugar source, the interaction of the two
Also extremely significant influence is produced on mda content, more significant influence is generated on Chlorophyll-a Content;Soluble protein in moss
More significant influence of the content by sugar source type, and the concentration of sugar source and its soluble protein can be contained with influencing each other for type
Amount generates extremely significant influence.
To sum up, sugar source is not suitable for applying in moss culture solution, and culture medium is easily caused to pollute, and inhibits moss growth breeding.
C, the physical signs of moss crust is shown in Fig. 8-11 when adding different plant growth regulator in optimal medium.
Fig. 8 is the response that moss Determination of Chlorophyll a content at any time changes plant growth regulator type and concentration.
The response that mda content at any time changes plant growth regulator type and concentration in Fig. 9 moss.
The response that soluble protein content at any time changes plant growth regulator type and concentration in Figure 10 moss.
Figure 11 is the response that soluble sugar content at any time changes plant growth regulator type and concentration in moss.
By Fig. 8-11 it can be seen that the interaction of plant growth regulator type, concentration and the two is to the leaf of moss
Green element a, soluble protein soluble sugar content, which have pole to write, to be influenced, and kind of the content of malonaldehyde by plant growth regulator
Class and its not significant with the influence of the interaction of concentration, but the influence moss that the concentration of plant growth regulator can be extremely significant
The content of middle malonaldehyde.
The type and concentration of plant growth regulator are significant to moss Effect of Physiology Characteristic, and moss is every when concentration is lower
Physical signs is superior under high concentration state, shows when low concentration that plant growth regulator can promote plant growth, when high concentration
There is apparent inhibiting effect.By observing for a long time, Initial stage of culture moss keep plant forms, but after incubation the phase when, plant
Significant degradation is cell state, attached wall, conglomeration situation occurs, has jade-green suspended matter, plant growth regulator in culture solution
Have the function of promoting plant cell differentiation, but does not observe in this experiment.Show plant growth regulator to desert tongue fur
The influence of the physiological property and cell differentiation of moss is not identical.
To sum up, 2, the 4-D that the 6-B-A or 0.5mg/L of 0.5mg/L are added in Hogland culture medium is suitable Shapotou
The optimum condition that regional moss indoors artificial is cultivated.Sugar source is not suitable for applying in moss culture solution, and culture medium is easily caused to pollute,
Inhibit moss growth breeding.Influence of the plant growth regulator to the physiological property and cell differentiation of desert muskeg is simultaneously asynchronous,
There is facilitation to the growth and breeding of moss.
(2) preparation of concave convex rod base high water-absorption resin
A, concave convex rod base high water-absorption resin is in distilled water when Figure 12 is the mass fraction of different heat modification concave convex rods
The water absorbent rate of different time in (pH6.22 or so).
B, concave convex rod base high water-absorption resin is in 0.9% sodium chloride when Figure 13 is the mass fraction of different heat modification concave convex rods
The water absorbent rate of different time in solution.
C, Figure 14 is the maximum water absorbent rate of concave convex rod base high water-absorption resin with the mass fraction degree of heat modification concave convex rod
And the variation tendency of solution ph.
D, Figure 15 is that different ultrasonic power interferes lower APT10 concave convex rod base high water-absorption resin on the distilled water (left side pH6.22
It is right) in different time water absorbent rate.
E, Figure 16 is that different ultrasonic power interferes lower APT10 concave convex rod base high water-absorption resin in 0.9% sodium chloride solution
The absorbency of middle different time.
The maximum water absorbent rate of concave convex rod base high water-absorption resin is in the raising of heat modification concave convex rod mass fraction degree
Downward trend after first rising, in APT10, the maximum water absorbent rate of concave convex rod base high water-absorption resin reaches maximum value.It is concave-convex
The maximum water absorbent rate of stick base high water-absorption resin is in downward trend after first rising, wherein not with the raising of solution ph
The maximum water absorbent rate change rate of the super absorbent resin of heat modification attapulgite is added close to 50%, adds heat modification concave convex rod
Soil reaches peak in neutral solution, and change rate is no more than 30%, shows that adding heat modification attapulgite facilitates
Improve the stability of super absorbent resin water absorbent rate in different pH value.
F, concave convex rod base high water-absorption resin water retention measurement result is shown in Table 1.
The variation tendency of concave convex rod base high water-absorption resin water retention under the mass fraction degree of the different heat modification concave convex rods of table 1
Variation tendency that concave convex rod base high water-absorption resin water-retaining property changes with heat modification concave convex rod mass fraction degree and its
Water absorbent rate variation tendency is consistent, and in APT10, the water conservation time of concave convex rod base high water-absorption resin reaches longest.Using
When ultrasonic interference method prepares concave convex rod base high water-absorption resin, ultrasonic power be 200W when water imbibition and inhale salt water performance most
It is good.
To sum up, it selects mass fraction is added and prepares concave convex rod base high water-absorption tree for 10% heat modification concave convex rod sample
Rouge.
(3) compounding of moss type concave convex rod based high molecular sand fixation material
A, Figure 17 is that moss Determination of Chlorophyll a content at any time fixes the sand to the moss type concave convex rod based high molecular of different compounding ratios
The response of material.
Note: capitalization indicates that, there are significant difference (p < 0.05) between incubation time, lowercase indicates the same time
There are significant differences between culture substrate.
B, influence of the different compoundings than lower moss type concave convex rod based high molecular sand fixation material to the development characteristics of moss crust
(growth 80 days) is shown in Table 2.
Influence of the different compoundings of table 2 than lower moss type concave convex rod based high molecular sand fixation material to the development characteristics of moss crust
Comprehensive all indexs consider that the optimum compound proportion of moss type concave convex rod based high molecular sand fixation material is 1:1, first tongue fur
The compounding of moss and concave convex rod base high water-absorption resin is compared for 1:1, and the physiological property of moss is most strong, and mossy cell is divided into plant
State is better, and skinning thickness and skinning compression strength are in medium level.
(4) performance test that fixes the sand of moss type concave convex rod based high molecular sand fixation material
A, Figure 18 is the moss knot of moss type concave convex rod based high molecular sand fixation material culture under different moisture content and nutritional condition
Skin Determination of Chlorophyll a content with/time changing rule.
Note: 1 indicates precipitation interval time (d) in 1-1:0 of legend title, and 1:0 indicates nutrient solution and distillation in supplement moisture content
The compounding ratio of water, the rest may be inferred for other legend titles.
B, influence of the different compoundings than lower moss type concave convex rod based high molecular sand fixation material to the development characteristics of moss crust
(growth 80 days) is shown in Table 3.
Under the different moisture content of table 3 and nutritional condition under moss type concave convex rod based high molecular sand fixation material culture moss crust hair
Educate feature (growth 80 days)
The desert muskeg for picking up from landscape in Shapotou area, the mossy cell obtained after mass rearing remain with certain drought resisting
Property, so growth conditions are good in drought environment;Mossy cell is to be suitable in the initial growth environment being sprayed on Shamian Island
The wet environment of moss growth, mossy cell can also preferably form moss crust, it is contemplated that as moss is in moist environment
The extension of middle growth time, drought resistance can be gradually reduced;It is fluctuated in water condition raw in biggish Semi-arid environment semi-moist
Long moss crust, growth conditions are worst in three kinds of situations in 80 days of observation, but do not differ excessive, desert muskeg sheet
Just extremely slowly, moss crust moistens drought environment condition in gradually Adaptable growth environment to the speed of growth of body in the observation period
It is alternately present the drought resistance that moss can be improved, while quickly being bred in moist environment, high is wanted to the adaptability of environmental change
The moss crust developed under other two kinds of environment.Can reasonable expectation by the moss crust cultivated in three kinds of initial nurturing an environment move
It is semi-moist to be rapidly adapted to the moss crust cultivated under drought condition after planting in desert.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (10)
1. a kind of preparation method of moss type concave convex rod based high molecular sand fixation material, it is characterised in that: include the following steps:
(1) pretreatment and expansion of desert moss crust are numerous:
A, the pretreatment of desert moss crust: desert moss crust is cleaned and sterilized;
B, the expansion of artificial culture moss crust is numerous:
The pretreated desert moss crust of step a is inoculated into culture medium and is cultivated;
The culture medium includes Hogland culture medium and plant growth regulator, and the plant growth regulator is 0.4-
The 2,4-D of the 6-B-A or 0.4-0.6mg/L of 0.6mg/L;
The culture is temperature is 30 DEG C, intensity of illumination is 120 μ E/ (m2·s1), light dark period be 12h:12h environment
Middle culture;
(2) preparation of the pretreatment of concave convex rod and concave convex rod base high water-absorption resin:
A, removal of impurities the pretreatment of concave convex rod: is cleaned after attapulgite is ground;
B, pretreated concave convex rod is subjected to heat modification;
C, the preparation of concave convex rod base high water-absorption resin:
By acrylic acid be added sodium hydroxide solution in, under ice-water bath in and, degree of neutralization 80%, be added acrylamide, then plus
Enter heat modification concave convex rod, obtains polymerized monomer;Crosslinking agent and initiator are added in monomer, stirred evenly, under certain condition
Fully reacting obtains concave convex rod base high water-absorption resin;
The crosslinking agent is N, N '-methylene-bisacrylamide;
The initiator is the potassium peroxydisulfate and sodium hydrogensulfite that molar ratio is 1:1;
(3) preparation of moss type concave convex rod based high molecular sand fixation material:
The concave convex rod base high water-absorption resin of the desert moss crust of step (1) preparation and step (2) preparation is uniformly mixed, is obtained
To moss type concave convex rod based high molecular sand fixation material.
2. according to the method described in claim 1, it is characterized by: the heat modification is the condition at 400 DEG C in step (2)
The lower heat modification for carrying out 1 hour.
3. according to the method described in claim 1, it is characterized by: in step (2), the addition quality of the heat modification concave convex rod
Percentage is 10%.
4. according to the method described in claim 1, it is characterized by: the dosage of the crosslinking agent is polymerized monomer in step (2)
The 0.05% of weight;The dosage of the initiator is the 0.6% of polymerized monomer weight.
5. according to the method described in claim 1, it is characterized by: in step (2), the fully reacting under certain condition is
It is reacted 60 seconds under 800W microwave power.
6. according to the method described in claim 1, it is characterized by: in step (2), the fully reacting under certain condition is
At 80 DEG C of bath temperature, reacted 120 minutes when ultrasonic power is 200W.
7. according to the method described in claim 1, it is characterized by: in step (2), the dosage of the acrylic acid, acrylic amine
Than for 15ml:4g.
8. according to the method described in claim 1, it is characterized by: desert moss crust and concave convex rod base height are inhaled in step (3)
The mass ratio of water-base resin is 1:1.
9. a kind of moss type concave convex rod based high molecular sand fixation material is using the described in any item method systems of claim 1-8
For what is obtained.
10. application of the moss type concave convex rod based high molecular sand fixation material in fixing the sand;
Preferably, described fix the sand are as follows: be sprayed at sand fixation material in the culture dish for being covered with sterile sand, Shamian Island is kept when initial
It is wet, it is cultivated, an every 1-3 days moisture of supplement and Hoglang nutrient solution, the Hoglang nutrient solution and distilled water
Volume ratio is 1:1.
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1693320A (en) * | 2005-05-20 | 2005-11-09 | 中国科学院长春应用化学研究所 | Process for preparing high water adsorbing resin |
CN102229804A (en) * | 2011-05-12 | 2011-11-02 | 西北师范大学 | Clay-based compound sand consolidating material |
CN104737875A (en) * | 2015-04-16 | 2015-07-01 | 黄凌燕 | Method for planting macadamia tree in rocky desertification soil |
CN105693934A (en) * | 2016-03-10 | 2016-06-22 | 南京博星科技有限公司 | Organic-inorganic composite super absorbent polymer |
CN108841393A (en) * | 2018-05-25 | 2018-11-20 | 苗霞明 | A kind of preparation method of biological breadcrust sand fixation material |
-
2018
- 2018-12-27 CN CN201811609574.3A patent/CN109504388A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1693320A (en) * | 2005-05-20 | 2005-11-09 | 中国科学院长春应用化学研究所 | Process for preparing high water adsorbing resin |
CN102229804A (en) * | 2011-05-12 | 2011-11-02 | 西北师范大学 | Clay-based compound sand consolidating material |
CN104737875A (en) * | 2015-04-16 | 2015-07-01 | 黄凌燕 | Method for planting macadamia tree in rocky desertification soil |
CN105693934A (en) * | 2016-03-10 | 2016-06-22 | 南京博星科技有限公司 | Organic-inorganic composite super absorbent polymer |
CN108841393A (en) * | 2018-05-25 | 2018-11-20 | 苗霞明 | A kind of preparation method of biological breadcrust sand fixation material |
Non-Patent Citations (2)
Title |
---|
曹田: "苔藓型凹凸棒基高分子固沙材料的制备与性能研究", 《兰州交通大学工程硕士学位论文》 * |
陶玲: "苔藓结皮复配凹凸棒基高吸水性固沙材料的生理特性", 《中国沙漠》 * |
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