CN109499093A - The extraction technique of amino acid in a kind of soybean - Google Patents
The extraction technique of amino acid in a kind of soybean Download PDFInfo
- Publication number
- CN109499093A CN109499093A CN201811458006.8A CN201811458006A CN109499093A CN 109499093 A CN109499093 A CN 109499093A CN 201811458006 A CN201811458006 A CN 201811458006A CN 109499093 A CN109499093 A CN 109499093A
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- extraction
- amino acid
- solvent
- soybean
- extracting solution
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0261—Solvent extraction of solids comprising vibrating mechanisms, e.g. mechanical, acoustical
- B01D11/0265—Applying ultrasound
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0288—Applications, solvents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0292—Treatment of the solvent
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Acoustics & Sound (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of extraction techniques of amino acid in soybean, its step are as follows: 1) fresh soyabean crushes, refluxing extraction is carried out twice to material using the mixture of petroleum ether or industrial naptha or both as solvent, the volume mass of solvent and material ratio is 3:2-18:2 when extraction, extraction time 8 hours, in extraction process to extractor carry out it is intermittent vacuumize and vacuum breaker, merge extracting solution twice and be concentrated, it is spare；Soybean liquid extracting solution after concentration is subjected to silica gel column chromatography, is then eluted with the mixture of n-hexane and ether.The present invention effectively destroys the cell wall of cell using the technology combined at complex enzyme, increases the mass transfer area that amino acids substance during subsequent extracted is spread to ethyl alcohol, significantly improves the recovery rate of amino acid.
Description
Technical field
The present invention relates to a kind of extraction process, in particular to the extraction technique of amino acid in a kind of soybean.
Background technique
Soybean (English name Garlic；Latin name AlliumSativum), it is Liliaceae (Liliaceous) allium
(Allium) the underground bulb of plant, there is 7~11 garlic cloves in soybean, narrow outer width in each garlic clove, white, plump succulence,
It is wrapped in film, scape surrounding growth is surrounded, to form oblate spheroid.Soybean has unique healthcare function, has perhaps to body
More benefits, all such drug effects be derived from its chemical component abundant, the toasted garlic of different sources has same drug effect
And chemical component, but due to geographical difference, the content of chemical component is different, the albumen to play a role in health care in people's garlic
Matter, amino acid, vitamin, sulfide equal size also just slightly have difference.1844 from the special Dorr pair difficult to understand of German famous chemist
Soybean is studied, the chemical component and catabolite progress that the chemist then from the various regions Hua Jie one after another has soybean
Research.The non-volatile compounds of soybean mainly include steroidal saponin, soap former times aglucon, amino acid class, phenols, hemagglutinin,
Levulan and various amino acid etc..Yellow gang of scoundrels's compound in soybean mainly has: Kaempferol, luteolin, apiolin, red bayberry
Glycosides etc..Amino acid is some secondary metabolites that plant generates during long-term natural selection, be widely present in vegetables,
In fruit and medicinal plant, it is a kind of active material generated through photosynthesis, there is the brittleness and infiltration for adjusting capillary
Property, protection cardiovascular system, it is anti-oxidant, eliminate free radical, reduce blood glucose, anti-aging, enhancing immune and antitumor action, anti-
A variety of physiological activity and the pharmacological actions such as infection, antibacterial, antiviral and antiallergy.Summary of the invention the purpose of the present invention is to provide
The extraction technique of amino acid, total amino acid is extracted from soybean, is combined using breaking-wall cell and enzymatic hydrolysis in a kind of soybean
Technology, have many advantages, such as easy to operate, it is high to pollute few reagent recovery utilization rate.
Summary of the invention
The present invention solve technical problem the following technical schemes are provided: in a kind of soybean amino acid extraction technique,
Steps are as follows: 1) fresh soyabean crushes, and flows back using the mixture of petroleum ether or industrial naptha or both as solvent to material
It extracting twice, the volume mass ratio of solvent and material is 3:2-18:2 when extraction, extraction time 8 hours, to mentioning in extraction process
Take tank carry out it is intermittent vacuumize and vacuum breaker, merge extracting solution twice and be concentrated, it is spare；Soybean liquid after concentration is extracted
Liquid carries out silica gel column chromatography, is then eluted with the mixture of n-hexane and ether, eluent is concentrated, decoloration, it is dry and
It is spare after crystallization；2) what the cutinase of zinc acetate, 1 .0% that 0 .3% of shell-broken liquid weight is added in the shell-broken liquid formed
Complex enzyme under alkaline condition after 5~6h of enzyme, obtains enzymolysis liquid for enzymolysis liquid after destroy the enzyme treatment and is homogenized addition alcoholic solvent ultrasound
Extraction obtains total amino acid extracting solution twice；3) it will be filtered after concentrate alcohol solvent, obtain ammonia after filtrate freeze-drying
Base acid.The invention has the advantages that the cell wall of cell is effectively destroyed, after increasing using the technology combined at complex enzyme
The mass transfer area that amino acids substance is spread to ethyl alcohol in continuous extraction process, significantly improves the recovery rate of amino acid；Through compound
Amino acids substance after enzymolysis processing carries out homogenized after mixing with ethyl alcohol, it is ensured that amino acid and alcohol solvent it is abundant
Mixing, and under the action of being homogenized high shear, cell generation is further crushed, and promotes mentioning for effective component amino acid
It takes.
Specific embodiment
Using specific embodiment, present invention will be explained in further detail below specific embodiment；In a kind of soybean
The extraction technique of amino acid, its step are as follows: 1) fresh soyabean crushes, with the mixing of petroleum ether or industrial naptha or both
Object is that solvent carries out refluxing extraction twice to material, and the volume mass ratio of solvent and material is 3:2-18:2 when extraction, when extraction
Between 8 hours, in extraction process to extractor carry out it is intermittent vacuumize and vacuum breaker, merge extracting solution twice and be concentrated, it is standby
With；Soybean liquid extracting solution after concentration is subjected to silica gel column chromatography, is then eluted, is washed with the mixture of n-hexane and ether
De- liquid is concentrated, decolourizes, is spare after dry and crystallization；2) acetic acid of 0 .3% of shell-broken liquid weight is added in the shell-broken liquid
Zinc, 1 .0% cutinase composition complex enzyme, under alkaline condition after 5~6h of enzyme, obtained after destroy the enzyme treatment enzymolysis liquid will
Enzymolysis liquid homogenate is added alcoholic solvent ultrasonic extraction and obtains total amino acid extracting solution twice；3) by mistake after concentrate alcohol solvent
Filter obtains amino acid after filtrate freeze-drying.
Claims (1)
1. the extraction technique of amino acid in a kind of soybean, its step are as follows: 1) fresh soyabean crushes, with petroleum ether or solvent
The mixture of gasoline or both is that solvent carries out refluxing extraction twice to material, and the volume mass ratio of solvent and material is when extraction
3:2-18:2, extraction time 8 hours, in extraction process to extractor carry out it is intermittent vacuumize and vacuum breaker, merge twice
Extracting solution is simultaneously concentrated, spare；Soybean liquid extracting solution after concentration is subjected to silica gel column chromatography, it is then mixed with n-hexane and ether
It closes object to be eluted, eluent is concentrated, decolourizes, is spare after dry and crystallization；2) shell-broken liquid weight is added in the shell-broken liquid
The complex enzyme for measuring the zinc acetate of 0 .3%, the cutinase composition of 1 .0%, under alkaline condition after 5~6h of enzyme, through destroy the enzyme treatment
After obtain enzymolysis liquid by enzymolysis liquid be homogenized be added alcoholic solvent ultrasonic extraction obtain total amino acid extracting solution twice；3) concentrate is used
It is filtered after alcohol solvent, obtains amino acid after filtrate freeze-drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811458006.8A CN109499093A (en) | 2018-11-30 | 2018-11-30 | The extraction technique of amino acid in a kind of soybean |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811458006.8A CN109499093A (en) | 2018-11-30 | 2018-11-30 | The extraction technique of amino acid in a kind of soybean |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109499093A true CN109499093A (en) | 2019-03-22 |
Family
ID=65749898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811458006.8A Withdrawn CN109499093A (en) | 2018-11-30 | 2018-11-30 | The extraction technique of amino acid in a kind of soybean |
Country Status (1)
| Country | Link |
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| CN (1) | CN109499093A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111111255A (en) * | 2019-11-14 | 2020-05-08 | 南开大学 | Method for extracting amino acid from soybean phloem |
-
2018
- 2018-11-30 CN CN201811458006.8A patent/CN109499093A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111111255A (en) * | 2019-11-14 | 2020-05-08 | 南开大学 | Method for extracting amino acid from soybean phloem |
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Legal Events
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190322 |