CN109498751A - A kind of Chinese medicine that preventing and treating Alzheimer disease-desert cistanche ginseng lotus rhizome of chuanxiong wisdom side and preparation method thereof - Google Patents

A kind of Chinese medicine that preventing and treating Alzheimer disease-desert cistanche ginseng lotus rhizome of chuanxiong wisdom side and preparation method thereof Download PDF

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CN109498751A
CN109498751A CN201910041795.3A CN201910041795A CN109498751A CN 109498751 A CN109498751 A CN 109498751A CN 201910041795 A CN201910041795 A CN 201910041795A CN 109498751 A CN109498751 A CN 109498751A
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魏芸
魏彤宇
刘聪
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Beijing University of Chemical Technology
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Abstract

The invention discloses a kind of Chinese medicines and preparation method thereof for preventing and treating Alzheimer disease, are made of the raw material of following mass fraction, 12-16 parts of Herba Cistanches, 8-12 parts of ginseng, 4-8 parts of Rhizoma Gastrodiae, and 4-9 parts and Rhizoma Chuanxiong 5-10 parts of the coptis.Can be made into granule, capsule, soft capsule, electuary, pill and soft extract, manufacture craft is simple, and it is at low cost, it is made completely of natural plants, it is hormone-free, other synthetics such as pigment are not added yet, after continuous use, are had no toxic and side effect to human body.The Chinese medicine contains the plurality of active ingredients such as the flavones being easily absorbed by the body, saponin(e, phenolic acid, alkaloid and polysaccharide, can effective for kidney-tonifying and brain tonic, QI invigoratingization is turbid, promoting blood circulation and detoxication, be suitable for the treatment of vital essence loss, ecchymosis aperture-blocking type mild cognitive impairment, Alzheimer disease (senile dementia) etc..

Description

It is a kind of prevent and treat Alzheimer disease Chinese medicine-desert cistanche ginseng lotus rhizome of chuanxiong wisdom side and its prepare Method
Technical field
The present invention relates to a kind of Chinese medicines and preparation method thereof for preventing and treating Alzheimer disease.
Background technique
Alzheimer disease (Alzheimer disease, AD) is that chronic progressive central nervous system degeneration becomes institute Caused dementia is the most common senile dementia, slowly to develop memory disorders, disorientation, linguistic function obstacle etc. no It is characterized with neuropsychic symptoms such as the cognition dysfunction of degree and abnormality of personality.AD is most common in nervous system degeneration disease Type, the morbidity of AD increases with the growth at age, and over-65s illness rate is 5%-10%；The above are 20%- within 85 years old 50%.With the aging of world population, the disease incidence of AD is on the rise, and the disease, which has become, at present jeopardizes old life The fourth-largest cause of disease (being only second to heart disease, tumour and apoplexy) of life becomes current gerontology and faces problem the severeest One of.Account for about the 35% of total population in China 65 years old and the above old man, 80 years old and the above old man are about 22%, and AD patient is expected It can reach 25,000,000, an important restriction factor for influencing family life quality and social development will be become.Due to the pathology of AD Mechanism is extremely complex, and the cause of disease is not still fully aware of at present, also lacks effective remedy measures.Therefore, the actively morbidity of research AD Mechanism, exploitation effectively prevent one of the vital task that AD drug is current infirmities of age research field.The viewpoint of China's traditional Chinese medicine Think: cognitive disorder, senile dementia belong to the scope of the disease such as " forgetful ", " mind disease ", " dementia ", " dementia ".Ancient medicine is to it Name of disease, etiology and pathogenesis, distinguishes to control etc. have many penetrating discussions at sick position.Ming Dynasty's Zhang Jingyue proposes " dementia " name of disease first, refers to Out " dementia it is all usually without phlegm and or with pent-up, or considered with unsuccessful or thought, or with feel uncertain or the heart gradually cause in terror it is dull-witted, Words are reverse, movement without or hidrosis, or kind anxious, it is strange that card then does surprise hundred, penetrates everywhere ", describe dull-witted performance multiplicity Clinical symptoms.Qing Dynasty's Wang Qingren errors in Medicine Corrected points out " Nian Gaowu memory person, brains are gradually empty ".In Qing Dynasty Wang Ang " collection of prescriptions with notes " Carry " essence of people and will are all hidden in kidney, and then ambition declines kidney essence deficiency, cannot on pass through the heart, therefore fascination is forgetful ".Cognition is prompted Obstacle, senile dementia sick position relevant to kidney essence deficiency, the cause of disease and the interpretation of the cause, onset and process of an illness, point out that kidney deficiency marrow sky is the main pathogenesis that it occurs Basis.Mild cognitive impairment, senile dementia basic pathogenesis are asthenia in origin and asthenia in superficiality, and void is mainly that kidney deficiency marrow is empty, and vital essence is insufficient, real main For phlegm is turbid, blood stasis, malicious heresy blind clear key.Therefore, kidney tonifying, essence replenishing QI invigorating, turbid removing toxic substances promoting blood circulation of dispelling, giving consideration to both the incidental and fundamental, be cognitive disorder, One of basic therapy of senile dementia.Clinically common marrow sea deficiency or caused by liver and kidney deficiency card type show as hypophrenia, look slow-witted Blunt, silencing is sayed less, burnout thinks sleeping, dizziness and tinnitus, soreness and weakness of waist and knees etc., pink tongue, and tongue fur is few or without tongue fur, and deeptensepulse is thin and delicate or string is thin etc.. Therefore treatment should with kidney tonifying, essence replenishing, QI invigoratingization is turbid, based on removing toxic substances promoting blood circulation.
The drug of AD can not treated at all still both at home and abroad at present, therefore early treatment is most important.The most commonly used gallbladder Alkali esterase inhibitor can only alleviate symptom, and cannot change the process of disease.This disease needs patient's Long-term taking medicine, western medicine result It is not good enough, and drug resistance is easily formed, adverse reaction is obvious；And have in terms of traditional Chinese medicine in prevention and treatment mild cognitive impairment and senile dementia Preferable clinical effectiveness embodies wholistic therapy, there is that multipath, too many levels are integrally-regulated, uses compound and dialectical opinion It controls, using natural drug, toxic side effect is small.Therefore, it is applied to A Er using the extraction of prepared slices of Chinese crude drugs compatibility and its active principle The prevention and treatment of Ci Haimo disease, the active drug for developing prevention and treatment AD have a very important significance.
Summary of the invention
The purpose of the present invention is to provide a kind of Chinese medicine compound prescriptions and preparation method thereof that can effectively prevent Alzheimer disease.
A kind of Chinese medicine that preventing and treating Alzheimer disease-desert cistanche ginseng lotus rhizome of chuanxiong wisdom side, by the raw material group of following mass fraction At 12-16 parts of Herba Cistanches, 8-12 parts of ginseng, 4-8 parts of Rhizoma Gastrodiae, 4-9 parts and Rhizoma Chuanxiong 5-10 parts of the coptis.The quality of each raw material Number can be with are as follows: 16 parts of Herba Cistanches, 12 parts of ginseng, 4 parts of Rhizoma Gastrodiae, 9 parts and 10 parts of Rhizoma Chuanxiong of the coptis.The mass fraction of each raw material It can be with are as follows: 13 parts of Herba Cistanches, 10 parts of ginseng, 6 parts of Rhizoma Gastrodiae, 6 parts and 9 parts of Rhizoma Chuanxiong of the coptis.The mass fraction of each raw material can be with Are as follows: 12 parts of Herba Cistanches, 9 parts of ginseng, 8 parts of Rhizoma Gastrodiae, 5 parts and 6 parts of Rhizoma Chuanxiong of the coptis.The mass fraction of each raw material can be with are as follows: meat 16 parts of desert cistanche, 12 parts of ginseng, 8 parts of Rhizoma Gastrodiae, 9 parts and 10 parts of Rhizoma Chuanxiong of the coptis.The mass fraction of each raw material can be with are as follows: Herba Cistanches 12 parts, 8 parts of ginseng, 4 parts of Rhizoma Gastrodiae, 4 parts and 5 parts of Rhizoma Chuanxiong of the coptis.Herba Cistanches are monarch drug in a prescription, nourishing liver and kidney, replenishing essence marrow in side；Minister is with ginseng QI invigorating qi-restoratives helps Herba Cistanches to mend the deficient of internal organs；Assistant is with Rhizoma Gastrodiae, coptis dissolving turbidity for resucitation, the poison for solving gods；Rhizoma Chuanxiong is to make medicine, living Blood stagnation resolvation and load medicine uplink are in brain.All medicine phases 5, play altogether tonify Qi of the kidney, promoting blood circulation and detoxication the effect of.This prevention and treatment Alzheimer The Chinese medicine of disease is the major functions: tonify Qi of the kidney, promoting blood circulation and detoxication, suitable for vital essence loss, ecchymosis aperture-blocking type cognitive disorder, senile dementia The treatment of (Alzheimer disease) etc..Symptoms include look stays blunt, forgetful or hypophrenia, can't take care of oneself, is dizziness and tinnitus, tired It is idle to think sleeping, pink tongue or there is ecchymosis, deeptensepulse is thin and delicate or string is counted accurately.
A kind of preparation method of Chinese medicine that preventing and treating Alzheimer disease, the preparation method include being prepared into Chinese materia medica preparation (dosage form comprising granule, capsule, soft capsule, electuary, pill, soft extract) and it is prepared into traditional Chinese medicine effective ingredient extract two Kind preparation method.The preparation method for being prepared into Chinese materia medica preparation includes following procedure: (1) take Herba Cistanches soak in water, then plus The ethyl alcohol for entering certain volume is decocted, is extracted；(2) ginseng, Rhizoma Gastrodiae, the coptis and Rhizoma Chuanxiong are taken, the extraction of 70% ethyl alcohol is added after mixing；(3) Merge step (1) and (2) resulting feed liquid, be concentrated under reduced pressure at 50-60 DEG C, centrifuge separation is merged centrate, mentioned using water Alcohol deposition method removes impurity, is then filtered, and filtrate is concentrated under reduced pressure at 50-60 DEG C into concentrated extracting solution；(4) step (3) institute It obtains concentrated extracting solution and auxiliary material is mixed and made into slurries, it is spray-dried that dry extract is made, auxiliary material granulation is added.The step (1) water of 8 times of quality is added to impregnate 2h in into Herba Cistanches, the ethyl alcohol for adding 8 times of quality extracts, time 3h.The step (2) it is stand-by in first plus 70% ethanol water of 10 times of quality flows back 1.5h, then to pour out 70% ethanol water, then plus 7 times of 70% ethyl alcohol of quality Secondary obtained alcohol extract is merged concentration by water reflux 1h.The revolving speed being centrifugated in the step (3) is 8000rpm.To step Suddenly drug made of (4) is dried, and drying temperature control is dried at 60 DEG C to 80 DEG C, and temperature is gradually increasing, mass water content Control is 2%.It crosses No.1 and screens out bulky grain, then cross No. five and screen out fine powder, dispensed after detection is qualified.
The preparation method for being prepared into traditional Chinese medicine effective ingredient extract includes following procedure: (1) by Herba Cistanches, ginseng, Rhizoma Gastrodiae, the coptis and Rhizoma Chuanxiong are uniformly mixed after crushing in proportion, ethyl alcohol are added, refluxing extraction is multiple, filters to take filtrate；It (2) will step Suddenly the dregs of a decoction in (1) add water refluxing extraction, add dehydrated alcohol to stand after merging Aqueous extracts, filtering；It (3) will be heavy in step (2) Starch merges with the filtrate in step (1) is concentrated to give medicinal extract；(4) preparation is made in medicinal extract together with auxiliary material.In the step (1) Reflow's cycle is 3 times, and each return time is 1.5h.
The present invention is a kind of Chinese medicine and preparation method thereof for preventing and treating Alzheimer disease, with Herba Cistanches, ginseng, Rhizoma Gastrodiae, Huang Even and Rhizoma Chuanxiong is raw material preparation, and manufacture craft is simple, at low cost, is made completely of natural plants, hormone-free, is not also added Other synthetics such as pigment, it is without side-effects to human body after continuous use.The Chinese medicine contain the flavones being easily absorbed by the body, The multiple nutritional components such as saponin(e, phenolic acid, alkaloid and polysaccharide, can effective for tonifying Qi of the kidney, promoting blood circulation and detoxication, be suitable for A Er The treatment of Ci Haimo disease.
Detailed description of the invention
Fig. 1 traditional Chinese medicine effective ingredient compatibility extraction process figure.
Fig. 2 prepared slices of Chinese crude drugs compatibility extraction process figure.
Specific embodiment
For a better understanding of the present invention, below with reference to the embodiment of the present invention, essence of the invention is further illustrated Property content, but the contents of the present invention are not limited thereto.
Embodiment 1
A kind of Chinese medicine compound prescription component preventing and treating Alzheimer disease, is made of, 16 parts of Herba Cistanches the raw material of following mass fraction, 12 parts of ginseng, 4 parts of Rhizoma Gastrodiae, 9 parts and 10 parts of Rhizoma Chuanxiong of the coptis.The preparation method of this Chinese medicine can be prepared into Chinese materia medica preparation, system Preparation Method includes following procedure as shown in Figure 1: (1) taking Herba Cistanches to soak in water, the ethyl alcohol for adding certain volume is decocted, mentioned It takes；(2) ginseng, Rhizoma Gastrodiae, the coptis and Rhizoma Chuanxiong are taken, the extraction of 70% ethyl alcohol is added after mixing；(3) it is resulting to merge step (1) and (2) Feed liquid, 50-60 DEG C be concentrated under reduced pressure, centrifuge separation, merge centrate, using water extraction and alcohol precipitation method remove impurity, then into Row filters, and filtrate is concentrated under reduced pressure at 50-60 DEG C into concentrated extracting solution；(4) concentrated extracting solution obtained by step (3) is mixed with auxiliary material Slurries are made, it is spray-dried that dry extract is made, auxiliary material granulation is added.Add 8 times of quality in the step (1) into Herba Cistanches Water impregnate 2h, add 8 times of quality ethyl alcohol extract, time 3h.First add 70% second of 10 times of quality in the step (2) Alcohol-water flow 1.5h, it is stand-by then to pour out 70% ethanol water, then plus 7 times of 70% ethanol waters of quality flow back 1h, by secondary obtained alcohol Extract merges concentration.The revolving speed being centrifugated in the step (3) is 8000rpm.Drug made of step (4) is done Dry, drying temperature control is dried at 60 DEG C to 80 DEG C, and temperature is gradually increasing, and mass water content is controlled 2%.This Chinese medicine can also be made At the dosage form of capsule, soft capsule, electuary, pill, soft extract.
Embodiment 2
A kind of Chinese medicine for preventing and treating Alzheimer disease, is made of, 12 parts of Herba Cistanches, 9 parts of ginseng the raw material of following mass fraction, 8 parts of Rhizoma Gastrodiae, 5 parts and 6 parts of Rhizoma Chuanxiong of the coptis.The preparation method of this Chinese medicine can be prepared into Chinese materia medica preparation, and (1) takes Herba Cistanches to add water It impregnates, the ethyl alcohol for adding certain volume is decocted, extracted；(2) ginseng, Rhizoma Gastrodiae, the coptis and Rhizoma Chuanxiong are taken, 70% second is added after mixing Alcohol extracting；(3) merge step (1) and (2) resulting feed liquid, be concentrated under reduced pressure at 50-60 DEG C, centrifuge separation merges centrifuge separation Liquid removes impurity using water extraction and alcohol precipitation method, is then filtered, and filtrate is concentrated under reduced pressure at 50-60 DEG C into concentrated extracting solution； (4) concentrated extracting solution and auxiliary material obtained by step (3) are mixed and made into slurries, spray-dried that dry extract is made, and auxiliary material system is added Grain.In the step (1) into Herba Cistanches plus the water of 8 times of quality impregnates 2h, the ethyl alcohol for adding 8 times of quality is extracted, and the time is 3h.In the step (2) first plus 70% ethanol water of 10 times of quality flows back 1.5h, it is stand-by then to pour out 70% ethanol water, then plus 7 times Secondary obtained alcohol extract is merged concentration by 70% ethanol water of quality reflux 1h.The revolving speed of centrifuge separation is in the step (3) 8000rpm.Drug made of step (4) is dried, drying temperature control is dried at 60 DEG C to 80 DEG C.This Chinese medicine can be made into The dosage form of granule, capsule, soft capsule, electuary, pill, soft extract.
Embodiment 3
A kind of drug matching preventing and treating Alzheimer disease, is made of, 16 parts of Herba Cistanches, ginseng the raw material of following mass fraction 12 parts, 8 parts of Rhizoma Gastrodiae, 9 parts and 10 parts of Rhizoma Chuanxiong of the coptis.The preparation method of this Chinese medicine can be prepared into traditional Chinese medicine effective ingredient extraction Object, preparation method is referring to shown in Fig. 2, including following procedure: (1) by Herba Cistanches 16 part, ginseng 12 part, Rhizoma Gastrodiae 8 part, the coptis 9 Part and 10 parts of Rhizoma Chuanxiong crush after be uniformly mixed, according to solid-liquid ratio 1: 15 ratio be added 70% (v/v) ethyl alcohol, flow back 3 times, every time Return time is 1.5h, merging filtrate；(2) water of 8 times of volumes of the dregs of a decoction in step (1) is flowed back 2 times, each 1.5h, is closed And add 4 times of 95% ethyl alcohol of volume to stand after Aqueous extracts, filtering；(3) sediment in step (2) and the filtrate in step (1) are closed And it is concentrated to give medicinal extract.This Chinese medicine can be made into the dosage form of granule, capsule, soft capsule, electuary, pill, soft extract.
Pharmacological effect experiment has been carried out according to the Chinese medicine that embodiment 1 synthesizes:
Experiment 1: influence of the traditional Chinese medicinal components of the present invention to APP transgenosis dementia mice
1 material
3 monthly age of 1.1 experimental animal APP695V717I transgenic mice 60 (half male and half female), weight 17-23g, cleaning grade, phase It is normal control with genetic background C57BL/6J mouse 15.Animal licensing number SCXK capital 2005-0013.
1.2 experimental drug positive control drugs select Doneppezil Hydrochloride (product name: Aricept), 5mg/ piece, when use It crushes, is dissolved with distilled water.This experiment also using acetylcholine, acetylcholinesterase, cholinacetyltranslase and enzyme-linked is exempted from Epidemic disease kit.Following " Chinese medicines of the present invention " refer to the drug in embodiment 1.
1.3 key instrument DMS-2 type Morris water maze instrument automatic data collections and processing system, STT-2 mouse diving tower Instrument (or DT-200 diving tower auto testing instrument), BH-2 type biomicroscope, DpxView Pro type computer color vision processing system System.
2 methods
2.1 grouping with administration according to random digit (according to SPSS statistical software generate random digit) by APP transgenic mice with Machine be divided into model group, big group of Chinese medicine of the present invention (9.76g crude drug/kg/d), Chinese medicine group of the present invention (4.88g crude drug/kg/d) and Doneppezil Hydrochloride group (0.65mg/kg/d), every group 15；The same monthly age C57BL/6J mouse 15 of identical genetic background is only used as Normal group.Normal group and model group give isometric distilled water stomach-filling, one time a day.Each group intragastric administration on mice 24 weeks, until September Behaviors survey is carried out when age.
2.2 Behavior test 2.2.1Morris water maze test Morris water maze pool diameter 120cm, pond are deep 50cm.Pond is divided into four quadrant areas with four equidistant points when experiment, is located among pool wall and the center of circle in fourth quadrant, Place organic glass platform.Into pond plus water, the depth of water about 20cm, the water surface are higher by platform surface 0.5cm, 25+1 DEG C of water temperature, infuse Enter milk powder, mixes, make water at opaque milky, achieve the purpose that animal vision can not distinguish that whether there is or not platforms in pond, and make Computer capacity successfully tracks the activity of mouse.Test carries out in the room of sound insulation, and pond position, light source position and intensity etc. are each Kind experimental condition remains unchanged in the entire experiment process, so that mouse comes locating platform position.Test program includes: (1) fixed Position navigation experiment: for measuring learning and memory ability of the animal in water maze.It carries out 1 time daily, continuous training 5 days.5th Remove platform its afternoon, mouse is put into pond, camera system automatically records mouse by an optional place of entry towards pool wall The number in disengaging original platform area and fourth quadrant swimming time and distance where original platform are used as evaluation mouse study in 3min The index of Memory result.
2.2.2 step down test diving tower instrument experimental box is connected with computer automatic production record.Mouse is put into jump when experiment In platform instrument experimental box, environment 5min is adapted to, is later put down mouse on diving tower gently, and copper grid are powered, when mouse is from diving tower It will receive electric shock when jumping off four limbs contact copper grid, normal avoiding reaction is to jump onto diving tower to return to safety zone, escapes electric shock, so learns 5min is practised, and records the electric shock number (errors number) in this 5min, as school grade.Carry out memory capability survey afterwards for 24 hours Examination, mouse is placed on diving tower, record mouse from rest on diving tower up to jump off for the first time time i.e. incubation period for being shocked by electricity and Errors number (number that mouse four limbs contact the electric shock of copper grid simultaneously) in 5min, in this, as the evaluation index of memory function.
2.3 brain tissue samples handle mouse after last Behavior test, fasting 12h, carry out brain tissue and serum materials. Eyeball takes blood after each group mouse anesthesia, is centrifuged (2500r/min, 15min), and separation serum maintains the temperature at 4 DEG C in case subsequent finger Mark detection；Quickly broken end takes full brain at low temperature, clears up bloodstain with ice-cold normal saline, is blotted with filter paper, is weighed.Every group random It chooses fixed above for 24 hours (4 DEG C) in 6 4% paraformaldehyde solutions of investment, changes rear fixer later, after brain tissue sinking i.e. Frozen section or paraffin embedding can be carried out, pathology and immunohistochemical staining are carried out.Remaining (in addition to small dose group is 7, other It is 8) mouse is rapidly separated cortex and hippocampus (carrying out on ice cream) respectively, and it is each that 2ml physiological saline (distilled water) homogenate is added, The centrifugation of low-temperature and high-speed centrifuge, separates supernatant, measures for tissue content.During stomach-filling, Chinese medicine small dose group dead 1 Only；In step down test, Western medicine group has 1 to get an electric shock and die.
3 experimental results
Compared with normal group, model group exists for influence of the 3.1 traditional Chinese medicinal components compatibilities to APP transgenic mice Spatial memory ability Original platform position number, fourth quadrant swimming time and distance are passed through in Morris water maze substantially reduces (P < 0.01).Respectively After administration group treatment, original platform position number, fourth quadrant swimming time and distance are passed through in Morris water maze compared with mould Type group significantly increases (P < 0.05/P < 0.01).It the results are shown in Table 1.
Influence of the 1 traditional Chinese medicinal components compatibility of table to APP transgenic mice Spatial memory ability
Note: compared with model group:*P < 0.05；**P < 0.01；Compared with only normal group:▲P < 0.05；▲▲P < 0.01.
3.2 traditional Chinese medicinal components compatibilities exist to the influence each group mouse of the disposable avoiding reaction memory function of APP transgenic mice There was no significant difference (P > 0.05) for errors number comparison among groups in step down test, but model group is presented what errors number increased Trend.Compared with normal group, the incubation period of model group is obviously shortened (P < 0.01)；Compared with model group, Doneppezil Hydrochloride group Incubation period is obviously prolonged (P < 0.05), and big group of incubation period of Chinese medicine of the present invention significantly extends (P < 0.01), Chinese medicine group of the present invention Also there is extension trend in incubation period, but there was no significant difference (P > 0.05).It the results are shown in Table 2.
Influence of the 2. traditional Chinese medicinal components compatibility of table to the disposable avoiding reaction memory function of APP transgenic mice
Note: compared with model group:*P < 0.05；**P < 0.01；Compared with normal group:▲P < 0.05；▲▲P < 0.01.
3.3 traditional Chinese medicinal components are to acetylcholine in APP hippocampus of mice, cholinacetyltranslase and acetylcholinesterase content It influences
Ach is reduced in the APP transgenic mice hippocampus at 9 monthly ages, and compared to the blank group, the content of model group Ach is substantially reduced, Difference is obvious (P < 0.05)；Compared with model group, donepezil, large and small group of Chinese medicine of the present invention can improve the content (P of Ach < 0.05).AchE activity increases in the APP transgenic mice hippocampus at 9 monthly ages, compared to the blank group, statistically significant (P < 0.01).Compared with model group, large and small group of Chinese medicine of the present invention can reduce the content (P < 0.05) of AchE；9 monthly ages CHAT activity reduces in APP transgenic mice hippocampus, and compared to the blank group, the content of model group CHAT is substantially reduced (P < 0.01)；Compared with model group, donepezil, large and small group of Chinese medicine of the present invention can improve the content of CHAT, all have statistics Meaning (P < 0.05).It the results are shown in Table 3.
Influence of 3 traditional Chinese medicinal components of table to September age APP transgenic mice hippocampus Ach, AchE, CHAT content
Note: compared with model group, * P < 0.05, * * P < 0.01
Influence of 3.4 traditional Chinese medicinal components to acetylcholine, cholinacetyltranslase and acetylcholinesterase content in cortex
Ach is reduced in the APP transgenic mice cortex at 9 monthly ages.Compared to the blank group, the content of model group Ach is substantially reduced (P < 0.05)；Compared with model group, donepezil, large and small group of traditional Chinese medicinal components of the present invention can improve the content of Ach；APP turns AchE content increases in DNA murine cortex, and compared to the blank group, model group AchE content obviously increases, obvious (the P < of difference 0.01).Compared with model group, donepezil, large and small group of traditional Chinese medicinal components of the present invention can reduce the content of AchE, but without statistics Learn meaning.The content of CHAT is reduced in the APP transgenic mice cortex at 9 monthly ages, and compared to the blank group, model group CHAT's contains Amount is substantially reduced (P < 0.05)；Compared with model group, donepezil, large and small group of Chinese medicine of the present invention can improve the content of CHAT (P < 0.05).It the results are shown in Table 4.
Influence of 4 traditional Chinese medicinal components of table to September age APP transgenic mice cortex Ach, AchE, CHAT content
Note: compared with model group, * P < 0.05
4 conclusions
PP transgenic mice is that app gene or genetic fragment are integrated into the genome of mouse using the method for microinjection, So that mouse brain expresses app gene.By APP albumen, overexpression, A β generate increase, neuron excitotoxicity are caused to be made in the brain With, damage ability of learning and memory in mice, and aggregation and deposition form senile plaque, to cause mouse senile dementia transgenosis Animal model.Result of study shows compared with Normal group, passes through original platform position in APP transgene mice model group 3min The number set substantially reduces (P < 0.01), and the fourth quadrant residence time where original platform substantially reduces (P < 0.01), swimming Distance significantly shortens (P < 0.05), illustrates that its ability of learning and memory is substantially reduced.Compared with model group, Chinese medicine of the present invention is large and small Group mouse traversing times dramatically increase (P < 0.05), dramatically increase (P < in the residence time of fourth quadrant and swimming distance 0.05/P < 0.01), show that each dosage of the traditional Chinese medicine effective ingredient can significantly improve the impaired learning and memory energy of APP transgenic mice Power.Step down test is the test for reflecting mouse one-trial passive avoidance response memory function.Compared with model group, Chinese medicine of the present invention Big group incubation period is obviously prolonged (P < 0.01), and also there is extension trend in Chinese medicine group of the present invention incubation period, but there was no significant difference (P > 0.05), show that traditional Chinese medicine effective ingredient has improvement to make the relatively long-term memory function in the short-term memory of APP transgenic mice With.To APP transgenic mice behaviouristics from carrying out in terms of Morris water maze and step down test two, APP695V717I turns DNA murine space ability of learning and memory and one-trial passive avoidance response memory capability at September age are remarkably decreased；Chinese medicine has Effect component early intervention can obviously improve the ability of learning and memory that APP transgenic mice has declined, to its Spatial memory and One-trial passive avoidance response memory capability tool is significantly improved.The content of Ach can be improved in the traditional Chinese medicine effective ingredient, increases The activity of strong CHAT, inhibits the activity of AchE.Improve APP transgenosis Model of Dementia pathology, cholinergic system and Apoptosis Etc. index, this may be its main curative effect mechanism and action pathway, have the function of preventing and treating Alzheimer disease.
The above embodiments are only used to help understand application of the invention and its core concept.It should be pointed out that pair For those skilled in the art, without departing from the principle of the present invention, the present invention can also be carried out Some improvements and modifications, these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims (9)

1. a kind of Chinese medicine for preventing and treating Alzheimer disease-desert cistanche joins lotus rhizome of chuanxiong wisdom side, it is characterised in that: it is by following mass fraction Raw material composition, 12-16 part of Herba Cistanches, 8-12 parts of ginseng, 4-8 parts of Rhizoma Gastrodiae, 4-9 parts of the coptis with Rhizoma Chuanxiong 5-10 parts.
2. Chinese medicine-desert cistanche of prevention and treatment Alzheimer disease joins lotus rhizome of chuanxiong wisdom side as described in claim 1, it is characterised in that described each The mass fraction of raw material are as follows: 15 parts of Herba Cistanches, 9 parts of ginseng, 6 parts of Rhizoma Gastrodiae, 5 parts and 6 parts of Rhizoma Chuanxiong of the coptis.
3. preventing and treating the preparation side, Chinese medicine-desert cistanche ginseng lotus rhizome of chuanxiong wisdom side of Alzheimer disease as described in claims 1 or 2 is any Method, it is characterised in that:
(1) Herba Cistanches are taken to soak in water, the ethyl alcohol for adding certain volume is decocted, extracted；
(2) ginseng, Rhizoma Gastrodiae, the coptis and Rhizoma Chuanxiong are taken, the extraction of 70% ethyl alcohol is added after mixing；
(3) merging step (1) and (2) resulting feed liquid, be concentrated under reduced pressure at 50-60 DEG C, centrifuge separation merges centrate, Impurity is removed using water extraction and alcohol precipitation method, is then filtered, filtrate is concentrated under reduced pressure at 50-60 DEG C into concentrated extracting solution；
(4) concentrated extracting solution and auxiliary material obtained by step (3) are mixed and made into slurries, spray-dried that dry extract is made, and are added auxiliary Material granulation.
4. preparation method as claimed in claim 3, it is characterised in that: add 8 times of quality in the step (1) into Herba Cistanches Water impregnates 2h, and the ethyl alcohol for adding 8 times of quality extracts, time 3h.
5. the preparation method of prevention and treatment Alzheimer disease Chinese medicine as claimed in claim 3, it is characterised in that: in the step (2) First plus 70% ethanol water of 10 times of quality flows back 1.5h, and it is stand-by then to pour out 70% ethanol water, then plus 7 times of 70% ethanol waters of quality return 1h is flowed, secondary obtained alcohol extract is merged into concentration.
6. the preparation method of prevention and treatment Alzheimer disease Chinese medicine as claimed in claim 3, it is characterised in that: in the step (3) The revolving speed of centrifuge separation is 8000rpm.
7. the preparation method of prevention and treatment Alzheimer disease Chinese medicine as claimed in claim 3, it is characterised in that: step (4) is made Drug be dried, drying temperature control is dried at 60 DEG C to 80 DEG C, and temperature is gradually increasing, and mass water content is controlled 2%.
8. preventing and treating the preparation method of Alzheimer disease Chinese medicine as described in claims 1 or 2 is any, it is characterised in that: it includes Following procedure:
(1) it is uniformly mixed after crushing Herba Cistanches, ginseng, Rhizoma Gastrodiae, the coptis and Rhizoma Chuanxiong in proportion, alcohol reflux extracts, and filters to take Filtrate；
(2) dregs of a decoction in step (1) are added into water refluxing extraction, adds dehydrated alcohol to stand after merging Aqueous extracts, filtering；
(3) sediment in step (2) is merged with the filtrate in step (1) and is concentrated to give medicinal extract,
(4) preparation is made in medicinal extract together with auxiliary material.
9. preparation method as claimed in claim 8, it is characterised in that: reflow's cycle is 3 times in the step (1), is returned every time The stream time is 1.5h.
CN201910041795.3A 2019-01-16 2019-01-16 A kind of Chinese medicine that preventing and treating Alzheimer disease-desert cistanche ginseng lotus rhizome of chuanxiong wisdom side and preparation method thereof Pending CN109498751A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102078460A (en) * 2011-01-14 2011-06-01 中国中医科学院西苑医院 Traditional Chinese medicine for preventing and treating Alzheimer disease and preparation method thereof
CN108514582A (en) * 2018-07-12 2018-09-11 中国中医科学院西苑医院 A kind of component compatibility Chinese medicine of prevention Alzheimer disease

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102078460A (en) * 2011-01-14 2011-06-01 中国中医科学院西苑医院 Traditional Chinese medicine for preventing and treating Alzheimer disease and preparation method thereof
CN108514582A (en) * 2018-07-12 2018-09-11 中国中医科学院西苑医院 A kind of component compatibility Chinese medicine of prevention Alzheimer disease

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