CN109497353A - Application of the graphene oxide in preparation Lepidoptera and dipteral insect feed - Google Patents

Application of the graphene oxide in preparation Lepidoptera and dipteral insect feed Download PDF

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CN109497353A
CN109497353A CN201811430915.0A CN201811430915A CN109497353A CN 109497353 A CN109497353 A CN 109497353A CN 201811430915 A CN201811430915 A CN 201811430915A CN 109497353 A CN109497353 A CN 109497353A
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graphene oxide
concentration
feed
water
added
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CN109497353B (en
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王秀平
解海翠
李婧实
沈江洁
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Hebei Normal University of Science and Technology
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Hebei Normal University of Science and Technology
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements

Abstract

The invention discloses a kind of application of graphene oxide in preparation Lepidoptera and dipteral insect feed.Wherein, graphene oxide and Lepidoptera and dipteral insect feeding quality volume ratio (μ g/mL) are 500:1-2000:1.The feed is prepared by the following method: preparation graphene oxide solution prepares culture medium, culture medium is dissolved in graphene oxide solution, the dipteral insect feed based on graphene oxide is prepared.Of the invention Lepidoptera and dipteral insect based on graphene oxide can shorten the development of insect with feed, improve survival rate of larvae, increase larva and pupa weight, and greatly reduce time and production cost in actual application.

Description

Application of the graphene oxide in preparation Lepidoptera and dipteral insect feed
Technical field
The invention belongs to technical field of plant protection, specifically, be related to a kind of graphene oxide in preparation Lepidoptera and Application in dipteral insect feed.
Background technique
With the development of science and technology with the progress of nanotechnology, nano material is before every field is presented and is widely applied Scape.Graphene oxide is a kind of novel two-dimentional carbon nanomaterial, has preferable electricity, mechanics and optics etc. excellent special Property, in biomedical, sensing, environmental science, the fields such as engineering science show wide application prospect.In recent years, it aoxidizes Graphene causes the broad interest of all circles scholar in the application of agriculture field, as graphene oxide can load agricultural chemicals Reach the function of sustained release and targeting, to improve the utilization rate of agricultural chemicals, and reduces the side effect to non-target organism.So And graphene oxide is made an addition into insect feedstuff, the application as insect growth promotor is rarely reported.
The large scale artificial breeding of insect is not only one of the important means of modern control of insect research and natural products money The important channel that source obtains.Using natural feed raising for trying insect, due to by seasonality and the limit that must repeatedly replace feed System, is extremely difficult to the purpose of above-mentioned mass rearing.Meridic diet, which has, is not subject to seasonal restrictions and is not easy mouldy, rotten, exsiccation, And the features such as can changing less during the feeding process or not change feed, management is very convenient, and becoming large-scale industrialized production can Energy.However existing man-made feeds there are breeding cycles it is long, insect individual living or death rate is low, individual polypide constitutions are poor the problems such as, it is right The large-scale breeding of insect causes certain puzzlement.
Summary of the invention
In view of this, the present invention provides a kind of graphene oxides in preparation Lepidoptera and dipteral insect feed Using.
In order to solve the above-mentioned technical problem, the invention discloses a kind of graphene oxides in preparation Lepidoptera and Diptera elder brother Application in worm feed.
Optionally, graphene oxide and Lepidoptera and dipteral insect feeding quality volume ratio (μ g/mL) are 500:1- 2000:1。
Optionally, comprising the following steps:
Step 1, preparation graphene oxide solution: graphene oxide is dissolved in ionized water and is ultrasonically treated;
Step 2 prepares culture medium: corn flour is soluble in water, it is stand-by to be tuned into paste;Agar is soluble in water, it boils and stirs Mix thawing;White sugar is added after agar sufficiently melts, stirring makes sufficiently to dissolve, and stirring makes sufficiently to dissolve;Paste corn flour is fallen Enter in the agar water of sugaring, stir and is cooked into paste;Propionic acid stirring is added;Yeast powder is added after slightly cooling down, is stirred with glass bar Mixing makes to add pure water after mixing and make constant volume, and culture medium is prepared;
Step 3, dipteral insect feed of the preparation based on graphene oxide: culture medium is dissolved in step 1 and is prepared Graphene oxide solution in, the dipteral insect feed based on graphene oxide is prepared.
Optionally, the mass volume ratio (mg/ml) of the graphene oxide in the step 1 and ionized water is 100:30- 400:30。
Optionally, the mass ratio of corn flour and water is 10:38.
Optionally, the mass ratio of agar and water is 1:20;The mass ratio of white sugar and agar is 9:1.
Optionally, the volume mass of propionic acid and corn flour ratio (ml/g) is 1:20.
Optionally, the mass ratio of yeast powder and corn flour is 7:100.
Optionally, the volume mass of the total volume after constant volume and corn flour ratio (ml/g) is 17:2.
Optionally, the volume ratio of the culture medium in step 3 and the ionized water in step 1 is 170:30.
Compared with prior art, the present invention can be obtained including following technical effect:
The larva that of the invention Lepidoptera and dipteral insect feed based on graphene oxide can shorten insect is raw It is long to go through the phase, improve survival rate of larvae, increase larva and pupa weight, and time and production are greatly reduced in actual application Cost.
Certainly, it implements any of the products of the present invention it is not absolutely required to while reaching all the above technical effect.
Detailed description of the invention
The drawings described herein are used to provide a further understanding of the present invention, constitutes a part of the invention, this hair Bright illustrative embodiments and their description are used to explain the present invention, and are not constituted improper limitations of the present invention.In the accompanying drawings:
Fig. 1 is that the graphene oxide of various concentration of the present invention goes through phase influence to corn borer larvae;
Fig. 2 is that the graphene oxide of various concentration of the present invention goes through phase influence to corn borer pupa;
Fig. 3 is that the graphene oxide of various concentration of the present invention goes through phase influence to corn borer adult;
Fig. 4 is influence of the graphene oxide of various concentration of the present invention to corn borer egg laying amount;
Fig. 5 is influence of the graphene oxide to the hatching rate of egg stage of Ostrinia furnacalis of various concentration of the present invention;
Fig. 6 is the survival rate of larvae of Ostrinia furnacalis under difference GO concentration of the invention;
Fig. 7 is the percentage of pupation of Ostrinia furnacalis under difference GO concentration of the invention;
Fig. 8 is the eclosion rate of Ostrinia furnacalis under difference GO concentration of the invention;
Fig. 9 is the larval weight of Ostrinia furnacalis under difference GO concentration of the invention;
Figure 10 is the pupa weight of Ostrinia furnacalis under difference GO concentration of the invention.
Specific embodiment
Carry out the embodiment that the present invention will be described in detail below in conjunction with embodiment, whereby to the present invention how application technology hand Section solves technical problem and reaches the realization process of technical effect to fully understand and implement.
The invention discloses a kind of application of graphene oxide in preparation Lepidoptera and dipteral insect feed.
Graphene oxide and Lepidoptera and dipteral insect feeding quality volume ratio (μ g/mL) are 500:1-2000:1.
The preparation method of the invention also discloses a kind of dipteral insect feed based on graphene oxide, including it is following Step:
Step 1, preparation graphene oxide solution: graphene oxide is dissolved in ionized water and is ultrasonically treated, stone is aoxidized The mass volume ratio (mg/ml) of black alkene and ionized water is 100:30-400:30;
Step 2 prepares culture medium: being that 10:38 is soluble in water by corn flour according to mass ratio, it is stand-by to be tuned into paste;According to Mass ratio is that 1:20 is soluble in water by agar, boils stirring and melts;White sugar 135g is added after agar sufficiently melts, stirring makes to fill Divide dissolution, wherein the mass ratio of white sugar and agar is 9:1, and stirring makes sufficiently to dissolve;Paste corn flour is poured into the agar of sugaring In water, stirs and be cooked into paste;Propionic acid stirring is added, wherein the volume mass of propionic acid and corn flour ratio (ml/g) is 1:20;Slightly Chilly that yeast powder is but added afterwards, wherein the mass ratio of yeast powder and corn flour is 7:100, makes to add again after mixing with glass bar stirring Entering pure water makes constant volume, and culture medium is prepared, and the volume mass ratio (ml/g) of the total volume and corn flour after constant volume is 17:2;
Step 3, dipteral insect feed of the preparation based on graphene oxide: culture medium is dissolved in step 1 and is prepared Graphene oxide solution in, wherein the volume ratio of the ionized water in culture medium and step 1 is 170:30, is prepared and is based on The dipteral insect feed of graphene oxide.
Embodiment 1
Graphene oxide is dissolved in ionized water and is ultrasonically treated, the mass volume ratio of graphene oxide and ionized water It (mg/ml) is 100:30;It is that 10:38 is soluble in water by corn flour according to mass ratio, it is stand-by is tuned into paste;It is 1 according to mass ratio: 20 is soluble in water by agar, boils stirring and melts;White sugar 135g is added after agar sufficiently melts, stirring makes sufficiently to dissolve, In, the mass ratio of white sugar and agar is 9:1, and stirring makes sufficiently to dissolve;Paste corn flour is poured into the agar water of sugaring, is stirred And it is cooked into paste;Propionic acid stirring is added, wherein the volume mass of propionic acid and corn flour ratio (ml/g) is 1:20;After slightly cooling down Yeast powder is added, wherein the mass ratio of yeast powder and corn flour is 7:100, is made to add pure water after mixing with glass bar stirring Make constant volume, culture medium is prepared, the volume mass ratio (ml/g) of the total volume and corn flour after constant volume is 17:2;By culture medium It is dissolved in the graphene oxide solution that step 1 is prepared, wherein the volume ratio of culture medium and the ionized water in step 1 is The dipteral insect feed based on graphene oxide is prepared in 170:30.
Embodiment 2
Graphene oxide is dissolved in ionized water and is ultrasonically treated, the mass volume ratio of graphene oxide and ionized water It (mg/ml) is 200:30;It is that 10:38 is soluble in water by corn flour according to mass ratio, it is stand-by is tuned into paste;It is 1 according to mass ratio: 20 is soluble in water by agar, boils stirring and melts;White sugar 135g is added after agar sufficiently melts, stirring makes sufficiently to dissolve, In, the mass ratio of white sugar and agar is 9:1, and stirring makes sufficiently to dissolve;Paste corn flour is poured into the agar water of sugaring, is stirred And it is cooked into paste;Propionic acid stirring is added, wherein the volume mass of propionic acid and corn flour ratio (ml/g) is 1:20;After slightly cooling down Yeast powder is added, wherein the mass ratio of yeast powder and corn flour is 7:100, is made to add pure water after mixing with glass bar stirring Make constant volume, culture medium is prepared, the volume mass ratio (ml/g) of the total volume and corn flour after constant volume is 17:2;By culture medium It is dissolved in the graphene oxide solution that step 1 is prepared, wherein the volume ratio of culture medium and the ionized water in step 1 is The dipteral insect feed based on graphene oxide is prepared in 170:30.
Embodiment 3
Graphene oxide is dissolved in ionized water and is ultrasonically treated, the mass volume ratio of graphene oxide and ionized water It (mg/ml) is 400:30;It is that 10:38 is soluble in water by corn flour according to mass ratio, it is stand-by is tuned into paste;It is 1 according to mass ratio: 20 is soluble in water by agar, boils stirring and melts;White sugar 135g is added after agar sufficiently melts, stirring makes sufficiently to dissolve, In, the mass ratio of white sugar and agar is 9:1, and stirring makes sufficiently to dissolve;Paste corn flour is poured into the agar water of sugaring, is stirred And it is cooked into paste;Propionic acid stirring is added, wherein the volume mass of propionic acid and corn flour ratio (ml/g) is 1:20;After slightly cooling down Yeast powder is added, wherein the mass ratio of yeast powder and corn flour is 7:100, is made to add pure water after mixing with glass bar stirring Make constant volume, culture medium is prepared, the volume mass ratio (ml/g) of the total volume and corn flour after constant volume is 17:2;By culture medium It is dissolved in the graphene oxide solution that step 1 is prepared, wherein the volume ratio of culture medium and the ionized water in step 1 is The dipteral insect feed based on graphene oxide is prepared in 170:30.
Comparative example 1
Take 1 200ml beaker that 30ml deionized water is added;It is that 10:38 is soluble in water by corn flour according to mass ratio, is tuned into Paste is stand-by;It is that 1:20 is soluble in water by agar according to mass ratio, boils stirring and melt;White sugar is added after agar sufficiently melts 135g, stirring make sufficiently to dissolve, wherein the mass ratio of white sugar and agar is 9:1, and stirring makes sufficiently to dissolve;By paste corn flour It pours into the agar water of sugaring, stir and is cooked into paste;Propionic acid stirring is added, wherein the volume mass of propionic acid and corn flour ratio It (ml/g) is 1:20;Yeast powder is added after slightly cooling down, wherein the mass ratio of yeast powder and corn flour is 7:100, uses glass bar Stirring makes to add pure water after mixing and make constant volume, culture medium is prepared, the volume matter of total volume and corn flour after constant volume Amount is 17:2 than (ml/g);Culture medium is dissolved in step 1 ionized water, wherein the volume of the ionized water in culture medium and step 1 Than the dipteral insect feed based on graphene oxide is prepared for 170:30.
Illustrate technical effect of the invention below with reference to specific experimental data:
One, graphene oxide is to the longevity of corn borer and the influence of fecundity:
One) experimental method:
1, the measurement of corn borer longevity and egg laying amount and hatching rate:
The newly hatched larvae of Ostrinia furnacalis is loaded on prepared man-made feeds (fritter that feed is cut into 5*5cm) respectively In, every box feed connects worm 250, and records and connect the worm date.The feed for connecing worm is placed on culture (greenhouse item in growth cabinet Part: temperature: 28 DEG C, relative humidity 75%, photoperiod L ︰ D=12 ︰ 12), observation records the change of every larva until pupating daily The pupa date (larva goes through the phase);After larvae pupation, the pupa of change is chosen from feed daily, is placed it in the centrifuge tube of 15mL, Cotton balls is put in nozzle to get wet moisturizing, is collected in growth cabinet, until turning into insect imago, is recorded every pupa and is turned into The date of insect imago (pupa goes through the phase);After insect imago is sprouted wings, male and female pairing, which is placed in dixie cup, carries out raising until dead, observation Record the date of death (adult goes through the phase) of every insect imago;30 pairs of insect imagos that peak period is selected in each processing are laid eggs Test, repeats three times, record oviposition number and its hatching rate (egg laying amount and hatching rate).
2, Data Management Analysis method: all test measurements are completely randomized design, and each processing repeats three times.It is all Data are average value ± SE (standard error) for statistical analysis to experimental data using DPS software and Excel table, p value ﹤ 0.05 (or ﹤ 0.01), the t of use, which is examined, determines statistical significance.
Two) experimental result:
1, the graphene oxide of various concentration goes through the influence of phase to corn borer larvae
As seen from Figure 1, it in F0 generation, is not added with the corn borer larvae that the feed of graphene oxide is raised and goes through the phase and be 20 days, graphene oxide concentration was that the larva of feed (embodiment 1, similarly hereinafter) raised corn borer of 500 μ g/mL goes through the phase and is 18.33 days, 1000 μ g/mL concentration (embodiment 2, similarly hereinafter) were 17.17 days, and 2000 μ g/mL (embodiment 3, similarly hereinafter) are 16.33 days;In F1 generation, being not added with the corn borer larvae that the feed of graphene oxide is raised and going through the phase is 18.5 days, adds oxygen It is 18.17 days that the larva that graphite alkene concentration raises corn borer by the feed of 500 μ g/mL, which goes through the phase, and 1000 μ g/mL concentration are 16.17 days, concentration was that 2000 μ g/mL are 15.17 days;In F2 generation, it is not added with the jade that the feed of graphene oxide is raised It is 16.67 days that rice snout moth's larva larva, which goes through the phase, and the addition graphene oxide concentration larva of raising corn borer by the feed of 500 μ g/mL is gone through Phase is 13.67 days, and 1000 μ g/mL concentration are 13 days, and concentration is that 2000 μ g/mL are 11.83 days.From table 1 it follows that Compared with the concentration of control, graphene oxide concentration increases separately the Ostrinia furnacalis larvae raised under 1 times, 2 times and 4 times and goes through the phase There are extremely significant difference (p ﹤ 0.01).The graphene oxide of various concentration goes through the larva of corn borer as can be seen from the above results Phase has significant impact, and graphene oxide can shorten larva and go through the phase, and is in dosage effect.
2, the graphene oxide of various concentration goes through the influence of phase to corn borer pupa:
As seen from Figure 2, it in F0 generation, is not added with the corn borer pupa that the feed of graphene oxide is raised and goes through the phase and be 5.13 days, it was 5.50 days that the addition graphene oxide concentration pupa of raising corn borer by the feed of 500 μ g/mL, which goes through the phase, 1000 μ g/ ML concentration is 5.61 days, and 2000 μ g/mL are 5.82 days, in F1 generation, is not added with what the feed of graphene oxide was raised It is 6.51 days that corn borer pupa, which goes through the phase, and the addition graphene oxide concentration pupa of raising corn borer by the feed of 500 μ g/mL goes through the phase and is 6.30 days, 1000 μ g/mL concentration were 6.54 days, and concentration is that 2000 μ g/mL are 6.51 days;In F2 generation, it is not added with oxidation The corn borer pupa that the feed of graphene is raised go through the phase be 6.27 days, add graphene oxide concentration for 500 μ g/mL feed institute Raising corn borer pupa go through the phase be 6.6 days, 1000 μ g/mL concentration are 6 days, and concentration is that 2000 μ g/mL are 6.27 days.From table In 1 as can be seen that compared with the concentration of control, it is beautiful that graphene oxide concentration increases separately the Asia raised under 1 times, 2 times and 4 times Rice snout moth's larva pupa goes through the phase without significant difference (p ﹥ 0.05).As can be seen from the above results, the graphene oxide of various concentration is to corn borer Pupa go through the phase influence it is not significant.
3, the graphene oxide of various concentration goes through the influence of phase to corn borer adult
As seen from Figure 3, it in F0 generation, is not added with the corn borer adult that the feed of graphene oxide is raised and goes through the phase and be 4.11 days, it was 4.33 days that the addition graphene oxide concentration adult of raising corn borer by the feed of 500 μ g/mL, which goes through the phase, 1000 μ G/mL concentration is 4.08 days, and 2000 μ g/mL are 4.07 days;In F1 generation, the feed for being not added with graphene oxide is raised Corn borer adult to go through the phase be 3.75 days, addition graphene oxide concentration is raised the adult of corn borer by the feed of 500 μ g/mL Going through the phase is 3.47 days, and 1000 μ g/mL concentration are 3.71 days, and concentration is that 2000 μ g/mL are 3.90 days;In F2 generation, do not add The corn borer adult for adding the feed of graphene oxide to be raised go through the phase be 3.63 days, addition graphene oxide concentration be 500 μ g/mL The adult of the raised corn borer of feed to go through the phase be 3.9 days, 1000 μ g/mL concentration are 3.87 days, and concentration is 2000 μ g/mL It is 3.77 days.From table 1 it follows that graphene oxide concentration increases separately 1 times, 2 times and 4 times compared with the concentration of control The Ostrinia furnacalis adult of lower raising goes through the phase without significant difference (p ﹥ 0.05).As can be seen from the above results, the oxygen of various concentration It is not significant that graphite alkene goes through phase influence to the adult of corn borer.
The graphene oxide of 1 various concentration of table is to each generation larva of Ostrinia furnacalis, pupa and the variance analysis of Adult Development phase
4, influence of the graphene oxide of various concentration to corn borer egg laying amount
As seen from Figure 4, in F0 generation, being not added with the corn borer egg laying amount that the feed of graphene oxide is raised is 134.8, the addition graphene oxide concentration egg laying amount of raising corn borer by the feed of 500 μ g/mL is 166.02,1000 μ g/mL concentration is 183.31, and 2000 μ g/mL are 158.13;In F1 generation, it is not added with the feed institute of graphene oxide The corn borer egg laying amount of raising is 122.33, adds graphene oxide concentration by the feed of 500 μ g/mL and raises corn borer Egg laying amount is 129, and 1000 μ g/mL concentration are 134, and concentration is that 2000 μ g/mL are 126.33;In F2 generation, not The corn borer egg laying amount that the feed of addition graphene oxide is raised is 95.87, and addition graphene oxide concentration is 500 μ g/ The egg laying amount of the raised corn borer of the feed of mL is 97.84, and 1000 μ g/mL concentration are 106.43, and concentration is 2000 μ g/ ML is 108.77.From Table 2, it can be seen that graphene oxide concentration increases separately 1 times, 2 times compared with the concentration of control The Ostrinia furnacalis egg laying amount raised under with 4 times is without significant difference (p ﹥ 0.05).As can be seen from the above results, various concentration Graphene oxide influences the egg laying amount of corn borer not significant.
5, influence of the graphene oxide of various concentration to the hatching rate of egg stage of Ostrinia furnacalis
As seen from Figure 5, in F0 generation, it is not added with the hatching rate for the egg stage of Ostrinia furnacalis that the feed of graphene oxide is raised It is 65.33%, the hatching rate that addition graphene oxide concentration raises egg stage of Ostrinia furnacalis by the feed of 500 μ g/mL is 66.48%, 1000 μ g/mL concentration are 90.39%, and 2000 μ g/mL are 94.15%;In F1 generation, it is not added with the feed of graphene oxide The hatching rate for the egg stage of Ostrinia furnacalis raised is 89.7%, adds graphene oxide concentration by the feed of 500 μ g/mL and raises corn The hatching rate of snout moth's larva ovum is 88.18%, and 1000 μ g/mL concentration are 91.23%, and concentration is that 2000 μ g/mL are 91.4%;In F2 Dai Zhong, the hatching rate for being not added with the egg stage of Ostrinia furnacalis that the feed of graphene oxide is raised is 92.56%, and addition graphene oxide is dense Degree is 94.78% by the hatching rate that the feed of 500 μ g/mL raises egg stage of Ostrinia furnacalis, and 1000 μ g/mL concentration are 97.4%, dense Degree is that 2000 μ g/mL are 97.87%.From Table 2, it can be seen that egg stage of Ostrinia furnacalis can be increased compared with the concentration of control Hatching rate, but it is not significant in certain Generation differences.
Variance analysis of 2 graphene oxide of table to Ostrinia furnacalis each generation egg laying amount and hatching rate
Three) the study result show that, the graphene oxide of various concentration, which goes through the phase to the larva of Ostrinia furnacalis, to be had Significant impact.With the raising of graphene oxide concentration, Ostrinia furnacalis F0-F2 significantly contracts for the development duration of larva It is short.And graphene oxide goes through the phase to the pupa of corn borer, adult goes through the phase, egg laying amount and hatching rate do not have significant impact.
Two, graphene oxide influences the growth and development of Ostrinia furnacalis:
One) test method:
1, test process:
Test is arranged 3 GO concentration and handles, i.e. 2000 μ g/mL, 1000 μ g/mL and 500 μ g/mL.Preparing corn borer people The GO of three various concentrations is added during work feed, and not add the chow diet of GO as control, each processing repeats three It is secondary.Ostrinia furnacalis larvae raises the disinfection plastic casing that container is long 15cm, width 15cm and high 10cm, has diameter 4cm on box cover Circular hole, and with the gauze of 70 mesh stainless steels close circular hole, be put in growth cabinet and raise, the temperature inside the box: (28 ± 1) DEG C; Relative humidity (70 ± 10) %;Periodicity of illumination L: D=12h: 12h, (L, 6:00-18:00;D, 18:00-6:00).
2, the measurement of Ostrinia furnacalis survival rate:
In the man-made feeds that the newly hatched larvae access of the Ostrinia furnacalis hatched on the same day is prepared, 250, every box, record The worm date is connect, is placed in growth cabinet and raises, the growth and development state for the corn borer observed daily.To 5 before larvae pupation When age, all larvas of every box are picked out in the feed placed and newly configured, record larva sum calculates larva living or death rate (%);After all larvae pupations are complete, all pupa numbers are recorded, are calculated percentage of pupation (%);It is random from the pupa of every box respectively 5 are selected, the situation of its emergence is observed, is calculated eclosion rate (%).
3, the measurement of Ostrinia furnacalis whose body weight:
Larva of the same size (before pupating when 5 age) 15 is selected from every box respectively, larva worm weight is weighed, records original number According to;After larvae pupation (the 2nd day weighing pupa weight after pupating), every box selects pupa 15 of the same size and weighs pupa weight, records original Data, and the pupa changed will be all put into single head in the centrifuge tube of 15mL respectively daily and raised, it is marked with marking pen, and put Enter cotton balls and dip in water moisturizing, is put into disk in growth cabinet.
4, it statisticallys analyze
All test measurements are completely randomized design, and each processing repeats three times.Using DPS software and Excel table pair Experimental data is for statistical analysis, and the significance of difference carries out variance analysis using Duncar duncan's new multiple range method between each processing.
Two, result and analysis:
One) influence of the difference GO concentration to Ostrinia furnacalis survival rate
1, influence of the difference GO concentration to Ostrinia furnacalis larvae survival rate
As shown in fig. 6, the survival rate of larvae of control group is that 40.53%, GO concentration is 500 μ g/mL, 1000 μ in F0 generation When g/mL and 2000 μ g/mL, survival rate of larvae is respectively 42.53%, 48.13% and 51.20%, from table 3 it is observed that with Control group concentration is compared, and with the raising of GO concentration, there are extremely significant difference (p ﹤ for the raising of Ostrinia furnacalis larvae survival rate 0.01);In F1 generation, the survival rate of larvae of control group be 33.60%, GO concentration be 500 μ g/mL, 1000 μ g/mL and 2000 μ When g/mL, survival rate of larvae is respectively 38.67%, 42.40% and 48.67%, equally from table 3 it can be seen that dense with control group Degree is compared, and with the raising of GO concentration, survival rate of larvae improves (p ﹤ 0.01) significantly;In F2 generation, the larva of control group is deposited Motility rate be 38.40%, GO concentration be 500 μ g/mL, 1000 μ g/mL and 2000 μ g/mL when, survival rate of larvae is respectively 41.73%, 42.00% and 44.00%, it can be concluded that, compared with control group concentration, GO can increase corn borer children by table 3 The survival rate of worm, but difference is not significant (p ﹥ 0.05).
2, influence of the difference GO concentration to Ostrinia furnacalis percentage of pupation
As shown in fig. 7, F0 generation in, the percentage of pupation of control group be 32.80%, GO concentration be 500 μ g/mL, 1000 μ g/mL When with 2000 μ g/mL, percentage of pupation is respectively 40.80%, 43.87% and 45.87%, from table 3 it is observed that dense with control group Degree is compared, and with the raising of GO concentration, there are significant difference (p ﹤ 0.05) for the raising of Ostrinia furnacalis percentage of pupation;In F1 generation, The percentage of pupation of control group be 28.27%, GO concentration be 500 μ g/mL, 1000 μ g/mL and when 2000 μ g/mL, percentage of pupation is respectively 29.07%, 33.33% and 37.33%, equally from table 3 it is observed that compared with control group concentration, the raising pair of GO concentration The no significant impact of the raising of percentage of pupation (p ﹥ 0.05);In F2 generation, the percentage of pupation of control group is that 33.60%, GO concentration is When 500 μ g/mL, 1000 μ g/mL and 2000 μ g/mL, percentage of pupation is respectively 37.47%, 34.53% and 36.53%, by table 3 It can be concluded that GO does not make significant difference (p ﹥ 0.05) to Ostrinia furnacalis percentage of pupation compared with control group concentration.
3, influence of the difference GO concentration to Ostrinia furnacalis eclosion rate:
Fig. 8 shows, in F0 generation, the eclosion rate of control group be 87.91%, GO concentration be 500 μ g/mL, 1000 μ g/mL and When 2000 μ g/mL, eclosion rate is respectively 85.54%, 81.84% and 88.00%, from table 3 it is observed that with control group concentration It compares, the raising of GO concentration does not make significant difference (p ﹥ 0.05) to Ostrinia furnacalis eclosion rate;In F1 generation, the eclosion rate of control group It is 500 μ g/mL for 93.40%, GO concentration, 1000 μ g/mL and when 2000 μ g/mL, eclosion rate is respectively 92.33%, 89.19% With 88.67%, equally from table 3 it is observed that the raising of GO concentration has pole to the raising of eclosion rate compared with control group concentration Significantly affect (p ﹤ 0.01);F2 generation in, the eclosion rate of control group be 91.69%, GO concentration be 500 μ g/mL, 1000 μ g/mL When with 2000 μ g/mL, eclosion rate is respectively 93.69%, 94.11% and 96.27%.By in table 3 it can be concluded that, it is dense with control group Degree is compared, and GO has certain facilitation (p ﹤ 0.05) to the raising of eclosion rate.
Each generation larva living or death rate of Ostrinia furnacalis, percentage of pupation and eclosion rate variance analysis under 3 difference GO concentration of table
Two) influence of the difference GO concentration to Ostrinia furnacalis whose body weight
1, influence of the difference GO concentration to Ostrinia furnacalis larvae weight
Fig. 9 was shown in F0 generation, and the larval weight of control group is 0.0519g, and GO concentration is 500 μ g/mL, 1000 μ g/mL When with 2000 μ g/mL, larval weight is respectively 0.0617g, 0.0706g and 0.0814g;In F1 generation, the larva body of control group Weight is 0.0550g, and when GO concentration is 500 μ g/mL, 1000 μ g/mL and 2000 μ g/mL, larval weight is respectively 0.0733g, 0, 0784g and 0.0905g;In F2 generation, the larval weight of control group is 0.0508g, and GO concentration is 500 μ g/mL, 1000 μ g/mL When with 2000 μ g/mL, larval weight is respectively 0.0557g, 0.0618g and 0.0674g.As can be seen from Table 4, with control group Concentration is compared, and GO concentration increases separately the Ostrinia furnacalis larvae weight raised under 1 times, 2 times and 4 times, and there are extremely significant difference (p ﹤ 0.01).As can be seen from the above results, GO has significant facilitation to the increase of the larval weight of corn borer.
2, influence of the difference GO concentration to Ostrinia furnacalis pupa weight:
By that can know in Figure 10, in F0 generation, the pupa weight of control group is 0.0533g, and GO concentration is 500 μ g/mL, 1000 μ When g/mL and 2000 μ g/mL, pupa is respectively again 0.0631g, 0.0686g and 0.0739g, as can be seen from Table 4, with control group Concentration is compared, and with the raising of GO concentration, there are extremely significant difference (p ﹤ 0.01) for the increase of Ostrinia furnacalis pupa weight;In F1 generation In, the pupa weight of control group is 0.0495g, and when GO concentration is 500 μ g/mL, 1000 μ g/mL and 2000 μ g/mL, pupa is respectively again 0.0557g, 0,0645g and 0.0747g, equally as can be seen from Table 4, compared with control group concentration, the raising pair of GO concentration The increase of pupa weight has extremely significant influence (p ﹤ 0.01);In F2 generation, the pupa weight of control group is 0.0631g, and GO concentration is 500 μ When g/mL, 1000 μ g/mL and 2000 μ g/mL, pupa is respectively again 0.0502g, 0.0538g and 0.0576g, by that can obtain in table 4 Out, compared with the control group, GO does not make significant difference (p ﹥ 0.05) to Ostrinia furnacalis pupa again.
Each generation larva of Ostrinia furnacalis, pupal weight variance analysis under 2 difference GO concentration of table
In conclusion graphene oxide is as a kind of external factor for influencing insect growth, within the scope of a certain concentration, oxygen Graphite alkene can promote the increase of Ostrinia furnacalis weight, improve the survival rate and percentage of pupation of corn borer, at the same to pupa weight and Eclosion rate has an impact but influences not significant.
Above description has shown and described several preferred embodiments of invention, but as previously described, it should be understood that invention is not It is confined to form disclosed herein, should not be regarded as an exclusion of other examples, and can be used for various other combinations, modification And environment, and can be carried out within that scope of the inventive concept describe herein by the above teachings or related fields of technology or knowledge Change.And changes and modifications made by those skilled in the art do not depart from the spirit and scope of invention, then it all should be in the appended power of invention In the protection scope that benefit requires.

Claims (10)

1. application of the graphene oxide in preparation Lepidoptera and dipteral insect feed.
2. application according to claim 1, which is characterized in that graphene oxide and Lepidoptera and dipteral insect feed Mass volume ratio (μ g/mL) is 500:1-2000:1.
3. application according to claim 1, which comprises the following steps:
Step 1, preparation graphene oxide solution: graphene oxide is dissolved in ionized water and is ultrasonically treated;
Step 2 prepares culture medium: corn flour is soluble in water, it is stand-by to be tuned into paste;Agar is soluble in water, it boils stirring and melts Change;White sugar is added after agar sufficiently melts, stirring makes sufficiently to dissolve, and stirring makes sufficiently to dissolve;Paste corn flour is poured into and is added In the agar water of sugar, stirs and be cooked into paste;Propionic acid stirring is added;Yeast powder is added after slightly cooling down, is made with glass bar stirring Pure water is added after mixing makes constant volume, and culture medium is prepared;
Step 3, dipteral insect feed of the preparation based on graphene oxide: culture medium is dissolved in the oxygen that step 1 is prepared In graphite alkene solution, the dipteral insect feed based on graphene oxide is prepared.
4. application according to claim 3, which is characterized in that the matter of graphene oxide and ionized water in the step 1 Measuring volume ratio (mg/ml) is 100:30-400:30.
5. application according to claim 3, which is characterized in that the mass ratio of corn flour and water is 10:38.
6. application according to claim 3, which is characterized in that the mass ratio of agar and water is 1:20;White sugar and agar Mass ratio is 9:1.
7. application according to claim 3, which is characterized in that the volume mass of propionic acid and corn flour ratio (ml/g) is 1: 20。
8. application according to claim 3, which is characterized in that the mass ratio of yeast powder and corn flour is 7:100.
9. application according to claim 3, which is characterized in that the volume mass ratio of total volume and corn flour after constant volume It (ml/g) is 17:2.
10. application according to claim 3, which is characterized in that the culture medium in step 3 and the ionized water in step 1 Volume ratio is 170:30.
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