CN109497090A - Western pool streptomycete is promoting the application in Garlic Growth - Google Patents
Western pool streptomycete is promoting the application in Garlic Growth Download PDFInfo
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- CN109497090A CN109497090A CN201811560575.3A CN201811560575A CN109497090A CN 109497090 A CN109497090 A CN 109497090A CN 201811560575 A CN201811560575 A CN 201811560575A CN 109497090 A CN109497090 A CN 109497090A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The bacterium solution of the western pool streptomycete is sprayed at garlic plant basal part of stem when garlic bulb grows to 6 leaf to promote Garlic Growth by application of the western pool streptomycete (Streptomyces setonii) in promotion Garlic Growth.The present invention also provides western pool streptomycetes to prepare the application in Garlic Growth promotor, including as the western pool streptomycete of active constituent and carrier, the growth promoter is the promotor for promoting garlic plant height.To the west of pool streptomycete promote Garlic Growth, non agricultural chemical residuum, non-environmental-pollution do not cause damages to people and animals and soil, to the west of pool streptomycete fermentation liquid preparation Garlic Growth promotor, preparation process is simple, and at low cost, effect is good, easy to use.
Description
Technical field
The present invention relates to biological control and biological pesticides more particularly to western pool streptomycete to promote answering in Garlic Growth
With.
Background technique
Garlic (Allium sativum L.) is Liliaceae allium crop, is to produce with bulb (garlic), garlic stems and shoot
Product, it is full of nutrition, it is one of most popular conventional modulated product and important health food.China is the main production of garlic
State, planting garlic area expand year by year, and developing novel plant growth promoter is the new direction for solving the problems, such as Garlic Fertilization.It plants
Object endophyte is the resource microorganism having a extensive future, in nature due to that can generate rich and varied metabolite
In important ecological effects, cause the extensive concern of people.Endophyte of plant because with plant symbiosis, to plant growth
And the microenvironment of surrounding plants plays the role of adjusting and reparation.Bacillus (Bacillus spp.) is using more biological and ecological methods to prevent plant disease, pests, and erosion
One of bacterium, because it can generate the endospore of heat-resisting, drought-enduring, uvioresistant and organic solvent, so being ideal biological and ecological methods to prevent plant disease, pests, and erosion
Bacterium screens object.Common plant growth promoter such as gibberellin (GA), methyl α-naphthyl acetate (NAA), compound sodium nitrophenolate etc. are all to pass through chemistry
What synthetic method generated, synthesis cost is high, and it is complicated for operation, and not only cultivated simply using endophyte of plant, it is at low cost, and
Effect is good.
Summary of the invention
Of the existing technology in order to overcome the problems, such as, the present invention provides western pool streptomycete (Bacillus subtilis)
Promoting the application in Garlic Growth that the bacterium solution of the western pool streptomycete is sprayed at garlic when garlic bulb grows to 6 leaf
For plant basal part of stem to promote Garlic Growth, this constitutes the first aspect of the invention.
The present invention also provides western pool streptomycetes to prepare the application in Garlic Growth promotor, including as active constituent
Western pool streptomycete and carrier, the growth promoter be the promotor for promoting garlic plant height, this constitutes of the invention the
Two aspects.
Beneficial effects of the present invention: to the west of pool streptomycete promote Garlic Growth, non agricultural chemical residuum, non-environmental-pollution is right
People and animals cause security threat；To the west of pool streptomycete fermentation liquid preparation Garlic Growth promotor, preparation process is simple, cost
Low, effect is good, easy to use.
Western pool streptomycete of the invention be Streptomyces setonii strain AE78P, document source: Kim,
K.-O.,Shin,K.-S.,Kim,M.N.,Shin,K.-S.,Labeda,D.P.,Han,J.-H.,and Kim,S.B."
Reassessment of the status of Streptomyces setonii and reclassification of
Streptomyces fimicarius as a later synonym of Streptomyces setonii and
Streptomyces albovinaceus as a later synonym of Streptomyces globisporus
based on combined 16S rRNA/gyrB gene sequence analysis."
Int.J.Syst.Evol.Microbiol. the western pool streptomycete Streptomyces setonii of (2012) 62:2978-2985.
Strain AE78P can from Xuzhou City of Jiangsu Province brass hill area plant garlic phosphorus stem in by conventional method separation screening and obtain,
The applicant can also be contacted to ask for.From the application applying date in 20 years, the public can contact applicant and ask for applicant's statement
The strain.
Detailed description of the invention
Fig. 1 is the photo that bacterial strain 136 described in the embodiment of the present invention 2 produces auxin (IAA) in vitro；Wherein, left figure
The photo of IAA is produced for bacterial strain 136, right figure is LB blank control；
Fig. 2 is the photo that bacterial strain 136 promotes Garlic Growth in the greenhouse described in the embodiment of the present invention 3, wherein left
Figure is the control for not connecing 136 bacterium solution of bacterial strain, and right figure is the processing of 136 bacterium solution of inoculating strain.
Specific embodiment:
Embodiment 1: isolation and identification for strains
The garlic phosphorus stem of health is acquired in Xuzhou City of Jiangsu Province brass hill area, by conventional method separating payingoff bacteria, and separated
Purifying, obtaining one plant of pure strain number is 136.Using ISP2 culture be based on 28 DEG C, 200rpm shaking flask culture, later with
Thalline were collected by centrifugation by 8000rpm, then by multistage microwave amplifier phage gene group DNA, finally uses universal primer 27F:5'-
AGAGTTTGATCCTGGCTCAG-3'(sequence 2) and 1492R:5'-AAGGAGGTGATCCAGCCGCA-3'(sequence 3) carry out
The amplification of 16S rDNA gene PCR.PCR reaction system are as follows: 10 × buffer Mix (including Taq enzyme) 12.5 μ L, 10 μm of ol/L
0.5 10.5 μ L of μ L, ddH2O of 27F 0.5 μ L, 10 μm of ol/L1492R, 1 μ L of template DNA, pcr amplification product is through 0.8% agar
It after sugared detected through gel electrophoresis, send to Shanghai Sheng Gong Co., Ltd and is sequenced, sequencing is compared on the website NCBI and EzBio Cloud
As a result, comparison result is as shown in table 1, the bacterial strain and western pool streptomycete Streptomyces setonii strain AE78P bacterium
The similitude of strain is 100%.
The sequence alignment result of 1 number of table, 136 bacterial strain
Embodiment 2: bacterial strain 136 generates the measurement for promoting plant growth substance in vitro
There is the packing of nitrogen culture medium for prepared, every pipe fills 4Ml, is added in superclean bench after high pressure sterilization with filtering
The tryptophan solution of the 1mL2.5mL of degerming, makes the final concentration of tryptophan reach 0.5mg/Ml, and a small amount of strain to be tested 136 of picking connects
Kind is put in 28 DEG C of shaking table cultures after 7 days in test tube, draws 1mL bacterium solution suspension and 2mL colour developing is added into new centrifuge tube
Agent, then room temperature dark stands 20min, compares not connect the LB liquid medium of 136 bacterial strains, if compared with the control, connecing bacterium
There is pink and then shows that 136 bacterial strains produce IAA in processing, indicates that 136 cannot generate IAA if not occurring.There is nitrogen culture
Based formulas is as follows: 1L: glucose 10.0g；Yeast extract 0.5g；(NH4)2SO41.0g；K2HPO42.0g；MgSO40.5g；NaCl
0.1g；CaCO30.5g；pH 7.2.Color developing agent (ready-to-use) preparation method: taken in draught cupboard 15mL the concentrated sulfuric acid and
The deionized water of 25mL, the concentrated sulfuric acid is slowly added in deionized water, while being stirred rapidly and (being mixed, heat dissipation) with glass bar；To
After sulfuric acid solution is cooled to room temperature, then plus 0.5mol/L 750 μ L of ferric chloride solution, stand-by after mixing, as Salkowski ' s is aobvious
Toner.As shown in Fig. 2, pink occurs in the processing group of 136 bacterial strains of inoculation, show that 136 bacterial strains produce IAA.
Phos test media is prepared, is connect 136 single bacterium drop points and culture medium center, 30 DEG C of inversion trainings with sterilizing toothpick
It supports, can bacterium surrounding is observed after 3 days generate transparent circle, if there is then illustrating that the bacterial strain has solution Phos activity, record molten
Phosphorus loop diameter and colony diameter, the two ratio reaction solution the active power of Phos, if bacterium colony surrounding cannot generate it is transparent
Circle then shows that the bacterium is active (table 2) without solution Phos.Phos test cultures based formulas is as follows: glucose 10.0g, magnesium sulfate
0.25g, calcium phosphate 5.0g, potassium chloride 0.2g, ammonium sulfate 0.1g, glucose 10.0g, magnesium chloride 5.0g, agar powder 20.0g.
The ability of 2 136 bacterial strain of table generation growth-promoting substance
Embodiment 3: bacterial strain 136 promotes Garlic Growth measure of merit
Bacterium solution, is finally diluted to sterile water by the 136 bacterium solutions sterile washing 3 times that 28 DEG C of liquid ISP2 are shaken to training 7 days
108A cell/mL, so that endophyte processing is used.In 21 × 20 × 15cm3Flowerpot in sow garlic cultivar " improvement " phosphorus stem,
25 DEG C of illumination 16h/ dark 8h are cultivated 20-25 days, into the garlic bud phosphorus bud idiophase (6 leaf phase).Take 136 bacterial strain of 17mL
Then bacterium solution is covered with soil, for 24 hours, then 25 DEG C of illumination 16h/ are black for 25 DEG C of dark moisturizings along one circle of garlic plant basal part of stem spraying
Dark 8h is cultivated 15 days.Blank control does not connect endophyte.3 tests are repeated in 4 basins of each processing in total.The 15th day after connecing bacterium
Respectively statistics control and processing whole strain plant height, aerial part plant height, root long, aerial part fresh weight, root fresh weight and
Whole strain plant fresh weight.As shown in Fig. 2, compared with the control with the processed garlic plant of bacterium solution of bacterial strain 136, growing way is luxuriant, and
And blade is broad, shows that bacterial strain 136 is significant to the promotion growth result of garlic.In addition, western pool streptomycete can also be with nonionic table
Face activating agent such as tween, fatty amine, the mixing of glycerine fatty acid esters, using water as medium, ultramicro grinding is made thick flowable
Suspension, be sprayed on crop surface, it is adsorbable that different dispersions is formed in microparticle surfaces, improve plant basal part of stem biology
The distribution and infiltration of pesticide, increase operation rate.Secondly, can also be passed through in proportion abundant with inert filler and a certain amount of dispersing agent
After mixing, reach certain particle size.These powdered pesticides can directly impose on plant basal part of stem, be absorbed, and can also add water-wet
Profit, dispersion, are spray applied.Powdered pesticide has the characteristics that storage and transportation is convenient, safety, and packaging material is easily processed, to plant also compared with
Safety.
Sequence table
<110>Jiangsu Normal University
<120>western pool streptomycete is promoting the application in Garlic Growth
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1375
<212> DNA
<213>western pool streptomycete (Streptomyces setonii)
<400> 1
caaggggttg ggccaccggc ttcgggtgtt accgactttc gtgacgtgac gggcggtgtg 60
tacaaggccc gggaacgtat tcaccgcagc aatgctgatc tgcgattact agcaactccg 120
acttcatggg gtcgagttgc agaccccaat ccgaactgag accggctttt tgagattcgc 180
tccgcctcgc ggcatcgcag ctcattgtac cggccattgt agcacgtgtg cagcccaaga 240
cataaggggc atgatgactt gacgtcgtcc ccaccttcct ccgagttgac cccggcagtc 300
tcctgtgagt ccccatcacc ccgaagggca tgctggcaac acagaacaag ggttgcgctc 360
gttgcgggac ttaacccaac atctcacgac acgagctgac gacagccatg caccacctgt 420
ataccgacca caaggggggc accatctctg atgctttccg gtatatgtca agccttggta 480
aggttcttcg cgttgcgtcg aattaagcca catgctccgc tgcttgtgcg ggcccccgtc 540
aattcctttg agttttagcc ttgcggccgt actccccagg cggggaactt aatgcgttag 600
ctgcggcacc gacgacgtgg aatgtcgcca acacctagtt cccaacgttt acggcgtgga 660
ctaccagggt atctaatcct gttcgctccc cacgctttcg ctcctcagcg tcagtaatgg 720
cccagagatc cgccttcgcc accggtgttc ctcctgatat ctgcgcattt caccgctaca 780
ccaggaattc cgatctcccc taccacactc tagctagccc gtatcgaatg cagacccggg 840
gttaagcccc gggctttcac atccgacgtg acaagccgcc tacgagctct ttacgcccaa 900
taattccgga caacgcttgc gccctacgta ttaccgcggc tgctggcacg tagttagccg 960
gcgcttcttc tgcaggtacc gtcactttcg cttcttccct gctgaaagag gtttacaacc 1020
cgaaggccgt catccctcac gcggcgtcgc tgcatcaggc tttcgcccat tgtgcaatat 1080
tccccactgc tgcctcccgt aggagtctgg gccgtgtctc agtcccagtg tggccggtcg 1140
ccctctcagg ccggctaccc gtcgtcgcct tggtaggcca ttaccccacc aacaagctga 1200
taggccgcgg gctcatcctt caccgccgga gcttttaacc ccgtcccatg cgggacagag 1260
tgttatccgg tattagaccc cgtttccagg gcttgtccca gagtgaaggg cagattgccc 1320
acgtgttact cacccgttcg ccactaatcc accaccgaag cggcttcatc gttcg 1375
<210> 2
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 2
agagtttgat cctggctcag 20
<210> 3
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 3
aaggaggtga tccagccgca 20
Claims (5)
1. western pool streptomycete (Streptomyces setonii) is promoting the application in Garlic Growth.
2. western pool streptomycete according to claim 1 is promoting the application in Garlic Growth, which is characterized in that its specific packet
It includes, when garlic bulb grows to 6 leaf, the bacterium solution of the western pool streptomycete is sprayed at garlic plant basal part of stem.
3. the western pool streptomycete in claim 1 is preparing the application in Garlic Growth promotor.
4. western pool streptomycete according to claim 3 is preparing the application in Garlic Growth promotor, which is characterized in that institute
Stating Garlic Growth promotor is the promotor for promoting garlic plant strain growth.
5. western pool streptomycete according to claim 4 is preparing the application in Garlic Growth promotor, which is characterized in that institute
Stating Garlic Growth promotor includes as the western pool streptomycete of active constituent and carrier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811560575.3A CN109497090A (en) | 2018-12-20 | 2018-12-20 | Western pool streptomycete is promoting the application in Garlic Growth |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811560575.3A CN109497090A (en) | 2018-12-20 | 2018-12-20 | Western pool streptomycete is promoting the application in Garlic Growth |
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| CN109497090A true CN109497090A (en) | 2019-03-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811560575.3A Pending CN109497090A (en) | 2018-12-20 | 2018-12-20 | Western pool streptomycete is promoting the application in Garlic Growth |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112359003A (en) * | 2020-12-16 | 2021-02-12 | 西南林业大学 | Streptomyces thalictriformis strain and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010030582A2 (en) * | 2008-09-11 | 2010-03-18 | Emory University | Noscapine and noscapine analogs and their use in treating infectious diseases by tubulin binding inhibition |
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2018
- 2018-12-20 CN CN201811560575.3A patent/CN109497090A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010030582A2 (en) * | 2008-09-11 | 2010-03-18 | Emory University | Noscapine and noscapine analogs and their use in treating infectious diseases by tubulin binding inhibition |
Non-Patent Citations (2)
| Title |
|---|
| GOPALAKRISHNAN. S: "Evaluation of actinomycete isolates obtained from herbal vermicompost for the biological control of Fusarium wilt of chickpea", 《CROP PROTECTION》 * |
| 赵东利: "吲哚乙酸(IAA)对大蒜根生长发育的影响", 《大连大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112359003A (en) * | 2020-12-16 | 2021-02-12 | 西南林业大学 | Streptomyces thalictriformis strain and application thereof |
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Application publication date: 20190322 |