CN109497069B - Bactericide composition containing boscalid and flusilazole, preparation method and application thereof - Google Patents
Bactericide composition containing boscalid and flusilazole, preparation method and application thereof Download PDFInfo
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- CN109497069B CN109497069B CN201811586986.XA CN201811586986A CN109497069B CN 109497069 B CN109497069 B CN 109497069B CN 201811586986 A CN201811586986 A CN 201811586986A CN 109497069 B CN109497069 B CN 109497069B
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- flusilazole
- boscalid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
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Abstract
The invention discloses a bactericide composition containing boscalid and flusilazole, a preparation method and application thereof, wherein a boscalid raw medicament with the mass concentration of 85 percent and a flusilazole raw medicament with the mass concentration of 80 percent are respectively prepared into 1 multiplied by 10 by using methanol4Storing the mother liquor of mg/L at a low temperature of 4 ℃; preparing the boscalid mother liquor into a liquid medicine containing 100-400 mg/L boscalid by using 0.1% of Tween 20, and preparing the flusilazole mother liquor into a liquid medicine containing 80-200 mg/L flusilazole by using 0.1% of Tween 20; the boscalid and the flusilazole liquid medicine are mixed in equal volume, and the prepared composition contains 50-200 mg/L of boscalid and 40-100 mg/L of flusilazole. The invention can prevent and cure the mildew and rot of the tobacco leaves in the baking period, reduce the medication cost and reduce the pollution to the environment.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a bactericide composition containing boscalid and flusilazole, a preparation method of the bactericide composition containing boscalid and flusilazole, and application of the bactericide composition containing boscalid and flusilazole in preventing and treating mildew and rot disease of tobacco leaves in a baking period.
Background
Tobacco leaf mildew disease (tobaco pole rot) caused by Rhizopus oryzae Went et al pr. gel is a fungal disease which is easy to appear in the tobacco curing period, mainly occurs in the tobacco leaf yellowing period, usually white hypha appears at the stem base, black spores are quickly generated, and further spread to harm. In the process of baking tobacco leaves in the bulk curing barn, the bulk curing barn has the advantages of small space, high temperature, high humidity and fast air flow, and is beneficial to the occurrence and the popularization of the diseases. The disease has the characteristics of strong explosiveness and harmfulness, the incidence rate is up to 100 percent in serious cases, and the quality of the flue-cured tobacco leaves is reduced, the yield is reduced, and the benefits of tobacco growers are damaged. Rhizopus oryzae (Zygomycota), a Rhizopus (Rhizopus) fungus, which produces large numbers of cystospores during growth. As a plant pathogen, sporangiospores thereof can rapidly spread from an infected flue-curing barn to a peripheral flue-curing barn in the tobacco curing process, so that the tobacco mildew disease is epidemic. The inhibition of the germination of pathogenic bacteria spores is one of the key measures for preventing and treating the mildew and rot disease of tobacco leaves; in addition, the inhibition of the growth of pathogenic bacteria hyphae in leaves is the second key measure for preventing and treating the diseases.
At present, exploratory reports appear on the research on the prevention and treatment of the mildew disease of tobacco leaves. The chinese invention patent CN103404962A discloses a method for preventing tobacco from being burnt during the baking process of tobacco, which emphasizes to change the wind direction and properly reduce the humidity in the baking room, but does not propose a specific operation mode to reduce the humidity in the baking room. Chinese patent CN202653141U discloses a wind direction control system of a flue-cured tobacco curing barn, which emphasizes the regulation and control method of regulating the wind direction of a fan among a yellowing stage, a color fixing stage and a dry-rib stage, and further reduces the humidity of the curing barn to reduce the mildew and rot disease of tobacco leaves in the curing barn. The Chinese invention patent CN102068028A discloses an airflow alternate operation type flue-cured tobacco intensive curing barn curing process, which also prevents and controls the mildew and rot disease of tobacco leaves by enhancing air flow and dehumidifying. The Chinese invention patent CN106418640A discloses a method for preventing and treating mildew and rot of tobacco leaves in the baking process of an air-flow-rising type bulk curing barn, and the principle is also a method for achieving moisture removal and disease reduction by adjusting the air flow speed. In a word, the related methods for preventing and controlling the tobacco leaf rotten disease are based on the change of the baking process flow, the tobacco leaf rotten disease is prevented and controlled by reducing the humidity in the baking room, the tobacco leaf baking technology is required to be skilled, and the requirement on the technical level of a baking worker is high. If the operation is improper, the tobacco leaf curing of the full curing barn can be failed, and serious economic loss is caused.
Chemical control is the most economical and effective main measure in a plurality of control methods for controlling plant diseases. Therefore, an efficient and safe bactericide composition is found, and is used for the parts (petioles) of tobacco leaves which are easy to cause diseases in various airflow type bulk curing barn to prevent and cure the rotten diseases of the cured tobacco leaves, so that the safety of the tobacco leaves can be guaranteed, the yield and the quality of the tobacco leaves are improved, the economic loss of tobacco growers is reduced, the application space is wide, and the requirement for the safe and sustainable development of the tobacco is met.
Disclosure of Invention
The invention aims to overcome the defects and provide the bactericide composition containing boscalid and flusilazole, which can prevent and treat the mildew and rot of tobacco leaves in the baking period, reduce the medication cost and reduce the pollution to the environment.
The invention also aims to provide a preparation method of the bactericidal composition containing boscalid and flusilazole.
The invention also aims to provide application of the bactericidal composition containing boscalid and flusilazole in preventing and treating the mildew and rot disease of tobacco leaves in the baking period.
The invention relates to a bactericide composition containing boscalid and flusilazole, which comprises the following components in percentage by weight: the boscalid content is 50-200 mg/L, and the flusilazole content is 40-100 mg/L.
The bactericidal agent composition containing boscalid and flusilazole preferably comprises the following components in percentage by weight: the boscalid content is 80-100 mg/L, and the flusilazole content is 60-80 mg/L.
The bactericidal agent composition containing boscalid and flusilazole comprises the following components in parts by weight: the boscalid is a bactericide raw material with the mass concentration of 80%, and the flusilazole is a bactericide raw material with the mass concentration of 85%.
The preparation method of the bactericidal agent composition containing boscalid and flusilazole comprises the following steps:
(1) respectively preparing boscalid raw drug with mass concentration of 85% and flusilazole raw drug with mass concentration of 80% into 1 × 10 parts by using methanol4Preserving the mother liquor of mg/L at a low temperature of 4 ℃ for later use;
(2) preparing the boscalid mother liquor into a liquid medicine containing 100-400 mg/L boscalid by using 0.1% of Tween 20, and preparing the flusilazole mother liquor into a liquid medicine containing 80-200 mg/L flusilazole by using 0.1% of Tween 20;
(3) the boscalid and the flusilazole liquid medicine are mixed in equal volume, and the prepared composition contains 50-200 mg/L of boscalid and 40-100 mg/L of flusilazole.
The invention relates to application of a bactericide composition containing boscalid and flusilazole in preventing and treating mildew and rot disease of tobacco leaves in a baking period.
Compared with the prior art, the invention has obvious beneficial effects, and the technical scheme can show that: the boscalid and the flusilazole are respectively prepared into 1 multiplied by 10 by methanol4Mu.g/ml of the mother liquor, diluted at the time of use with sterile water containing 0.1% Tween 20. The composition can be sprayed directly to the tobacco petiole after picking, or sprayed to the petiole after binding rod and before going to kang, or sprayed directly to the petiole after going to kang. Aiming at the action site of the target fungus of the rotten disease of the tobacco flue-cured tobacco, the invention inhibits the infection of pathogenic bacteria, inhibits the generation and the germination of pathogenic bacteria spores and reduces the re-infection circulation times of the diseases. The boscalid and the flusilazole do not generate chemical reaction, so that the bactericidal composition has obvious synergistic or additive effect, has obviously improved bactericidal effect compared with a single agent, can reduce the medication cost and reduce the pollution to the environment; the potential drug resistance risk of pathogenic bacteria to boscalid and flusilazole can be reduced; can expand the bactericidal spectrum and has the effect of treating other pathogenic bacteria which can cause the rotten disease of the tobacco. The in vivo test shows that the composition of boscalid and flusilazole has good control effect on the mildew and rot disease of the flue-cured tobacco leaves, and can be used for disinfection treatment of the surrounding environment of a flue-curing barn.
Detailed Description
Example 1
A preparation method of a bactericide composition containing boscalid and flusilazole comprises the following steps:
(1) respectively preparing boscalid raw drug with mass concentration of 85% and flusilazole raw drug with mass concentration of 80% into 1 × 10 parts by using methanol4And storing the mother liquor of mg/L at a low temperature of 4 ℃ for later use.
(2) The boscalid mother liquor is prepared into a liquid medicine containing boscalid at 400mg/L by using 0.1% of Tween 20, and the flusilazole mother liquor is prepared into a liquid medicine containing flusilazole at 80mg/L by using 0.1% of Tween 20.
(3) The boscalid and the flusilazole liquid medicine are mixed in equal volume, and the prepared composition contains 200mg/L boscalid and 40mg/L flusilazole.
Example 2
A preparation method of a bactericide composition containing boscalid and flusilazole comprises the following steps:
(1) respectively preparing boscalid raw drug with mass concentration of 85% and flusilazole raw drug with mass concentration of 80% into 1 × 10 parts by using methanol4And storing the mother liquor of mg/L at a low temperature of 4 ℃ for later use.
(2) And (2) preparing the boscalid mother liquor into a liquid medicine containing 100mg/L boscalid by using 0.1% of Tween 20, and preparing the flusilazole mother liquor into a liquid medicine containing 200mg/L flusilazole by using 0.1% of Tween 20.
(3) The boscalid and the flusilazole liquid medicine are mixed in equal volume, and the prepared composition contains 50mg/L boscalid and 100mg/L flusilazole.
Example 3
A preparation method of a bactericide composition containing boscalid and flusilazole comprises the following steps:
(1) respectively preparing boscalid raw drug with mass concentration of 85% and flusilazole raw drug with mass concentration of 80% into 1 × 10 parts by using methanol4And storing the mother liquor of mg/L at a low temperature of 4 ℃ for later use.
(2) And (2) preparing the boscalid mother liquor into a liquid medicine containing boscalid 300mg/L by using 0.1% of Tween 20, and preparing the flusilazole mother liquor into a liquid medicine containing flusilazole 150mg/L by using 0.1% of Tween 20.
(3) The boscalid and the flusilazole liquid medicine are mixed in equal volume, and the prepared composition contains 150mg/L boscalid and 75mg/L flusilazole.
Test example: test of fungicidal composition of boscalid and flusilazole prepared in examples 1 to 3 for controlling mildew disease of flue-cured tobacco leaves
Collecting mature tobacco leaves (Yunyan 87), binding the tobacco leaves according to a normal baking procedure, and isolating the tobacco leaves by adopting a plastic film to expose the petiole part.
Treating a bactericide composition containing boscalid and flusilazole: the liquid medicine of the composition in the embodiment is uniformly sprayed to the tobacco leaf stalks bound with the rods by a sprayer, and the liquid medicine is dried and then put on a kang for baking in a normal procedure.
And (4) comparison treatment: spraying clear water uniformly to the tobacco petioles bound with the rods by using a sprayer, airing, putting into a kang, and baking in a normal procedure.
After baking, investigating the influence of each treatment on the occurrence of the mildew and rot disease of the tobacco leaves, measuring the length of disease spots at the leaf stalks, counting the disease incidence, and calculating the disease control effect of each treatment of the medicament composition according to the length of the disease spots.
The results of the tobacco leaf mildew disease prevention effect test show that the bactericide composition containing boscalid and flusilazole can well prevent and treat the tobacco leaf mildew disease of the baked tobacco, and the disease prevention and treatment effect reaches over 90.90%.
TABLE 1 controlling Effect of the Fungicide composition of the present invention on rotten disease of flue-cured tobacco leaves
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the present invention without departing from the technical spirit of the present invention.
Claims (1)
1. The application of a bactericide composition containing boscalid and flusilazole in preventing and treating mildew and rot disease of tobacco leaves in a baking period.
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CN109497069B true CN109497069B (en) | 2021-04-02 |
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Citations (1)
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CN106135265A (en) * | 2015-04-13 | 2016-11-23 | 南京华洲药业有限公司 | A kind of containing Boscalid and the bactericidal composition of bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane and application |
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CN106135265A (en) * | 2015-04-13 | 2016-11-23 | 南京华洲药业有限公司 | A kind of containing Boscalid and the bactericidal composition of bis(4-fluorophenyl)methyl(1H-1,2,4-triazol-1-ylmethyl)silane and application |
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