CN109496832A - A kind of Ba Danmu pollen production method and its device of use - Google Patents
A kind of Ba Danmu pollen production method and its device of use Download PDFInfo
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- CN109496832A CN109496832A CN201811508034.6A CN201811508034A CN109496832A CN 109496832 A CN109496832 A CN 109496832A CN 201811508034 A CN201811508034 A CN 201811508034A CN 109496832 A CN109496832 A CN 109496832A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01D—HARVESTING; MOWING
- A01D91/00—Methods for harvesting agricultural products
- A01D91/04—Products growing above the soil
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention intends to provide the device of a kind of Ba Danmu pollen production method and its use.By carrying out flower acquisition using flower picker, and sterilizing and drying is carried out using anther drying device, progress anther essence mentions after obtaining pollen, pollen packaging, it is very big that the technological means of pollen storage solves Ba Danmu flower amount, but it is few to bear fruit, low output, and reported anther drying time, stack the not applicable problem of the manufacturing parameters such as thickness, it is easy to operate with corollary apparatus, efficiently, it is practical, it can be mass Ba Danmu pollen, the pollen compatibility of production is high, pollination time is flexible, scientifical use can effectively improve Ba Danmu pollination rate, to improve fruit fruit-setting rate, the effect of the development of China's Ba Danmu industry can be effectively facilitated.
Description
Technical field
The present invention relates to pollen production method technical fields, specifically, the present invention relates to a kind of productions of Ba Danmu pollen
The technical field of method and its device of use.
Background technique
Artificial pollination technology can not only influence to make up on florescence bad climate factor on caused by fruit fertilization, and
And pollen compatibility is high, pollination time is flexible, scientifical use can effectively improve fruit fruit-setting rate.Current China pears, apple,
In the pollen preparation production of the tree species such as Kiwi berry, cherry, main pollen collecting method is anther after artificial or reagent removal machine takes powder
In certain temperature and humidity, dry loose powder in the time, preservation is regathered.Currently, for other tree species pollen preparation research report compared with
It is more, but still belong to blank in a bar denier flitch face.
Ba Danmu (Amygdalus communis L.) is rosaceae Lee subfamily peach category almond subgenus deciduous tree, scientific name
Almond.Protein and grease rich in Ba Danmu benevolence, are both nutritious tonifying good merchantable brand, are also important medicinal material, pharmaceutical
Way is the best seller of nut fruits in international trade extensively.Kashi Shache County area be the world normal region Ba Danmu and I
Unique main producing region of state Ba Danmu.
Ba Danmu is cross-pollinatd plant, and the florescence is shorter.In production, suitable pollinated variety need to be configured, and passes through honey
Bee pollination.Ba Danmu flower amount greatly, but bear fruit less, low output by Shache county area, average single plant yield only 2.5-3kg, and possesses generation
Still there is larger gap in the California, USA producing region of the advanced Ba Danmu industry in boundary compared to (average single plant yield 4.5-13kg).One side
Face, at the beginning of annual mid or late March to 4 months when the Ba Danmu florescence, strong chromatic number weather all can frequently occur in Kaxgar Prefecture.Dry
Wind and floating dust not only accelerate column cap dry, and pole is unfavorable for the sprouting of pollen, more causes honeybee that can not pollinate, seriously affect Ba Danmu
Pollination reduces orchard yield.Meanwhile often there is cold spell in later spring in Kaxgar Prefecture at the Ba Danmu florescence, to the bud and flower of tree body
It causes damages, often will lead to a large amount of underproduction of Ba Danmu and even have no harvest.On the other hand, Southern Xinjiang woods fruit cultivated area is big, respectively sets
The case where kind of flowering time is concentrated, and the Ba Danmu florescence bee colony to pollinate often occurs supply falls short of demand.Although cultivated area established practice
Mould, but bar denier wooden pipe reason is whole more extensive, since kind configuration is improper, causes chaotic kind, main breed and pollinated variety flower
The problems such as phase is inconsistent, compatibility is poor is still generally existing.
The Kaxgar Prefecture florescence is dry, even if temperature change is violent in one day in fair weather, 3-4 DEG C of morning, and noon
Up to 26-27 DEG C, post sunset temperature reduces again.So the manufacturing parameters such as reported flower plucking time, stacking thickness are in Sha
Vehicle county is relatively not applicable.The Ba Danmu florescence is just overlapped with wheat cropping season, and artificial and flower source is nervous, in conventional method the amount of labour used it is big,
Hua Yuanliang is big, operational extremely low in actual production.In addition, the anther characteristic of Ba Danmu, out dose, powder outlet quantity etc., with pears, apple
The tree species such as fruit have differences.Therefore, improving Ba Danmu pollination rate is urgently solved in current China Ba Danmu industry production and scientific research
Certainly the problem of.
Summary of the invention
Greatly for Ba Danmu flower amount, but Shache county area is beared fruit less, low output, and reported anther drying time stacks
The manufacturing parameters such as the thickness problem more not applicable in Shache County, the present invention intends to provide a kind of Ba Danmu pollen production method and
Its device used.Operation is easy and efficient for this method and corollary apparatus, practical, can be mass Ba Danmu pollen, production
Pollen compatibility is high, pollination time is flexible, scientifical use can effectively improve Ba Danmu pollination rate, bear fruit to improve fruit
Rate can effectively facilitate the development of China's Ba Danmu industry.
The present invention provides a kind of Ba Danmu pollen production method and relevant apparatus, Ba Danmu pollen production provided by the invention
Method are as follows:
1. flower acquires
It is base of picking flowers with the garden Ba Danmu that no disease and pests harm is in the florescence, Best Times of picking flowers are fair weather 11:00-
18:00, picking will open balloon-like petal.It when picking flowers, is nipped off from bennet using flower picker, the flower of picking is used
Aperture≤0.5cm mesh bag yarn is displayed.After every 30 minutes collect 1 time, quickly spreads in ventilation, cool place, is protected from light place, spread thickness
1.5cm。
2. anther is detached from
Counted with florescence, for 24 hours within complete de- anther.The petal obtained using anther seperator to step 1 is quick
After reagent removal, it is sieved 1 time with the sieve of 120 mesh, is immediately fed into drying device.
3. anther is dry
Sterilizing and drying is carried out using anther drying device, drying device is kept for 25-27 DEG C of temperature, relative humidity≤18%.
Tile 1 layer of template in drying bed, spreads anther thickness 1-5mm.It is in yellow, pollen grain gold to anther shell after dry 20-26h
Yellow is glossy, it is tack-free when, i.e., drying terminate.
4. anther essence mentions
Pollen obtained in step 3 is swept into pallet with brush, using pollen extractor essence jacquard weave medicine 3 times, is sufficiently received
Collect pollen.
5. pollen is packed
With kraft envelope dispense pollen, 10g/ bags.After sealing, every 10 packet is placed in the plastic packaging equipped with 100g silica-gel desiccant
In bag.
6. pollen storage
By the pollen after packaging, it is protected from light and is placed in -40 DEG C of ultra low temperature freezer.
Preferably, 25 DEG C of hothouse room temperature spread anther thickness < 3mm, and drying time is for 24 hours.
A kind of portable flower picker provided by the invention, including basket and plucking shears are picked, in plucking shears, arc-shaped
Serrated knife blade is fixedly installed in handle one end, and curved blade is fixedly installed in close handles one end, and arc-shaped handle and close handles are logical
It is hinged to cross fixed swivel bearing, mesh bag slot is set in serrated knife blade, and the detachable string bag is flexibly connected with mesh bag slot, the detachable string bag
Connection ring is arranged in side；It picks in basket, basket carries portion upper edge setting neckstrap on the body, and harness, the setting of basket body bottom is arranged in basket body two sides
Waistband, basket body inside are equipped with hanger plate, and array is equipped with hook on hanger plate, and hook and connection ring are adapted setting, basket body bottom end
Zipper is set, and two side lower part of basket body is arranged kit bag, is equipped with the fixing belt being adapted with arc-shaped handle on the outside of kit bag.
In the present invention, T-type item, T-type one end and the detachable string bag is arranged in connection ring opposite side on the detachable string bag
It is fixedly connected, the T-type other end and mesh bag slot are adapted setting.
In the present invention, arc-shaped handle arc range is set as between π -1.5 π, convenient on the outside of hanging tool bag.
In the present invention, close handles meet the setting of manpower structure feature, are subject to and the finger of manpower four is facilitated to protrude into.
In the present invention, hook rectangular array on hanger plate is arranged.
In the present invention, the up-narrow and down-wide trapezoidal shape of basket body.
In the present invention, zipper is detachable and interchangeable, is 15N by the carrying force upper limit.
In the present invention, tightness can be adjusted by buckle in shoulder belt, neckstrap and waistband.
In the present invention, safety cock, the rotatable setting of safety cock is arranged in the outer end of serrated knife blade.
In the present invention, bracket is 15N by the carrying force upper limit, and shape, overweight mutability shape can be adjusted flexibly.
The present invention provides a kind of Ba Danmu anther dry separation sterilizing unit, including hopper, drying bed, transmission mechanism and goes out
Hopper, hopper and drying bed one end are adapted setting, trapezoidal cunning by ladder chute fixed-link, ladder chute upper end and hopper
One end fixed-link of slot lower end and drying bed, the other end and blow tank fixed-link of drying bed, drying bed are obliquely installed, do
LED- bactericidal lamp is equipped with above dry bed, drying bed surrounding and top are respectively provided with transparent glass cover；The top of drying bed is equipped with brush board,
Brush board upper end is equipped with connection ring, and setting orientation pulley on the crossbeam of brush board, track is located at fixed setting right above drying bed two sides,
The gradient of track formed plane and the gradient of drying bed are consistent, and orientation pulley and track are adapted setting；The bottom end of brush board
Hairbrush is set, and hairbrush passes through electric lifting rod and brush board active link；Drying bed two sides be fixedly installed air hose, the one of air hose
End is communicated with the adjustable air-heater of temperature, and the air hose of drying bed two sides is equipped with air outlet, and air outlet direction is oppositely arranged, air outlet
Upper setting blade, blade are linked by sharf with air outlet axis, the other end and electric telescopic rod active link of air hose, electricity
Dynamic telescopic rod and blade loose-leaf fixed-link, blade loose-leaf and blade fixed-link；Sub-material baffle is set in blow tank；Transmission
Mechanism includes motor, connection ring, gear one, gear two, chain, and motor is axially connected with gear one, gear one by chain with
Two active link of gear, chain and connection ring fixed-link；Control panel and the adjustable air-heater of motor, temperature, LED- bactericidal lamp,
Electric telescopic rod, electric lifting rod are electrically connected.
In the present invention, bolt is arranged in the bottom end of hopper, and bolt is in embedded setting.
In the present invention, the side that drying bed connects hopper is higher than the side of drying bed connection blow tank, the inclination of drying bed
Angle is 15 ° to 20 °.
In the present invention, blow tank is arc setting.
In the present invention, sub-material baffle setting parallel with discharging groove center line.
In the present invention, the amplitude of accommodation of blade is 120 °.
In the present invention, connection ring is symmetrical arranged in brush board two sides, and connection ring and chain are adapted setting.In the present invention
In, control panel control includes the drum of electric telescopic rod, electric lifting rod, LED- bactericidal lamp, motor and the adjustable air-heater of temperature
Wind mode.
By implementing the specific summary of the invention of the present invention, can achieve it is following the utility model has the advantages that
By carrying out flower acquisition using flower picker, anther acquisition is carried out, and carry out using anther drying device
Sterilizing and drying, obtains carrying out after pollen that anther essence mentions, pollen packaging, the technological means of pollen storage solve Ba Danmu flower amount pole
Greatly, but Shache county area bear fruit less, low output, reported flower plucking time anther drying time stacks the productions ginseng such as thickness
The number problem more not applicable in Shache County, having corollary apparatus, operation is easy and efficient, practical, can be mass Ba Danmu
Pollen, the pollen compatibility of production is high, pollination time is flexible, and scientifical use can effectively improve Ba Danmu pollination rate, to improve
Fruit fruit-setting rate can effectively facilitate the effect of the development of China's Ba Danmu industry.
Detailed description of the invention
Fig. 1 is shown as the Ba Danmu pollen technological process of production.
Fig. 2 is shown as portable petal picking basket apparatus structure schematic diagram provided by the invention.
Fig. 3 is shown as the present invention and provides portable petal plucking shears structural schematic diagram.
Fig. 4 is shown as hanger plate structural schematic diagram provided by the invention.
1a- picking basket in Fig. 2-4,11a- basket body, 12a- hanger plate, 13- zipper, 14- kit bag, 141- fixing belt,
110- bracket, 111- neckstrap, 112- shoulder belt, 113- waistband, 121- hook, 2- plucking shears, 21- curved blade, 22- serrated knife
Sword, 23- circular arc handle, 231- close handles, the fixed swivel bearing of 24-, 25- mesh bag slot, 26- safety cock, the detachable net of 27-
Pocket, 271-T profile bar, 272- connection ring, 121- hook.
Fig. 5 is shown as anther dry classification sterilizing unit structural schematic diagram provided by the invention.
Fig. 6 is shown as the dry separation sterilizing unit top view of Ba Danmu anther provided by the invention.
Fig. 7 is shown as air hose and electric telescopic rod structural schematic diagram in dry separation sterilizing unit provided by the invention.
Fig. 8 is shown as brush board structural schematic diagram in dry separation sterilizing unit provided by the invention.
In Fig. 5-8,1- hopper, 11- bolt, 12- ladder chute, 2- drying bed, 21-LED- bactericidal lamp, 3- brush board, 31-
Hairbrush, 32- electric lifting rod, 33- connection ring, 34- orientation pulley, 35- track, 4- motor, 41- gear one, 42- gear two,
43- chain, 5- temperature is adjustable air-heater, 51- air hose, 52- air outlet, 53- blade, 54- electric telescopic rod, 55- sharf,
56- blade loose-leaf, 6- blow tank, 61- sub-material baffle, 7- control panel.
Fig. 9 is shown as different drying times to the influence diagram of Ba Danmu pollen germination rate.
Figure 10 is shown as different drying temperatures to the influence diagram of Ba Danmu pollen germination rate.
Figure 11 is shown as different and spreads thickness to the influence diagram of Ba Danmu pollen germination rate.
Figure 12 is shown as the surface chart that drying time and drying temperature influence Ba Danmu pollen germination rate.
Figure 13 is shown as drying temperature and spreads the surface chart that thickness influences Ba Danmu pollen germination rate.
Figure 14 is shown as drying time and spreads the surface chart that thickness influences Ba Danmu pollen germination rate.
Specific embodiment
With reference to embodiment, the embodiment of the present invention is furthur described in detail, but the present invention is not limited to
Following implementations.
Test site are as follows: Kashi Area of Xingjiang Autonomous Region Shache County is located at autonomous region, Xinjiang of China Uygur, border area, southwest, Kun Lun Mountain
Northern foot, the Pamir Mountain Area are in the south, the Yarkanthe River alluvial fan Plain middle and upper reaches between the Takla Makan Desert and the desert Bu Guli,
N37 ° 27 ' 30 of north latitude " to N39 ° 15 ", E76 ° 1 ' 57 of east longitude " to E77 ° 46 ' 30 ", height above sea level 1200m, Ba Danmu cultivate seeding row spacing
For 6m × 7m, tree-like is natural open centre shape.
Material: Ba Danmu is with the Ba Danmu main breed " leatheroid Ba Danmu " of Kashi Area of Xingjiang Autonomous Region Shache County and " rich bar of evening
Denier wood " is experimental material.
It is sucrose that the present invention uses, agar, distilled water, template, kraft envelope, silica-gel desiccant, glass slide, micro-
Mirror, beaker, glass bar, electrothermal furnace, triangular flask, constant incubator, culture dish, cotton swab, marking pen, water, spoon, thermometer etc. are equal
It can be bought by public channel, equipment employed in technique and instrument are the common equipment in this field.
What all material, reagent and the instrument selected in the present invention were all well known in the art, but reality of the invention is not limited
It applies, other some reagents well known in the art and equipment are applied both to the implementation of following implementation of the present invention.
One: Ba Danmu pollen production method of embodiment
The garden Ba Danmu that the florescence is in no disease and pests harm is base of picking flowers, and fair weather 11:00-18:00 uses plucking shears
Cooperation picking bag picking will open balloon-like petal.It when picking flowers, is nipped off from bennet, flower aperture≤0.5cm of picking
Mesh bag yarn display.After every 30 minutes collect 1 time, quickly spreads in ventilation, cool place, is protected from light place, spread thickness 1.5cm；To adopt
Take time to count, for 24 hours within complete de- anther.After the quick reagent removal of anther seperator, it is sieved 1 time, is stood with the sieve of 120 mesh
Sterilizing and drying is carried out using anther drying device, drying device is kept for 21 DEG C of temperature, relative humidity≤18%.It is put down in drying bed
1 layer of template is spread, anther thickness < 1mm is spread.It is in yellow to anther shell, pollen grain golden yellow is glossy, tack-free after 20h
When, i.e. drying terminates；The pollen on template is swept into pallet with brush, using pollen extractor essence jacquard weave medicine 3 times, sufficiently
Collect pollen；With kraft envelope dispense pollen, 10g/ bags.After sealing, every 10 packet is placed in the modeling equipped with 100g silica-gel desiccant
In envelope；By the pollen after packaging, it is protected from light and is placed in -40 DEG C of ultra low temperature freezer.
Two: Ba Danmu pollen production method of embodiment
The garden Ba Danmu that the florescence is in no disease and pests harm is base of picking flowers, and fair weather 11:00-18:00 uses plucking shears
Cooperation picking bag picking will open balloon-like petal.It when picking flowers, is nipped off from bennet, flower aperture≤0.5cm of picking
Mesh bag yarn display.After every 30 minutes collect 1 time, quickly spreads in ventilation, cool place, is protected from light place, thickness 1.5cm is spread, to adopt
Take time to count, for 24 hours within complete de- anther.After the quick reagent removal of anther seperator, it is sieved 1 time, is stood with the sieve of 120 mesh
Sterilizing and drying is carried out using anther drying device, drying device is kept for 21 DEG C of temperature, relative humidity≤18%.It is put down in drying bed
1 layer of template is spread, anther thickness < 2mm is spread.It is in yellow to anther shell, pollen grain golden yellow is glossy, tack-free after 22h
When, i.e. drying terminates；The pollen on template is swept into pallet with brush, using pollen extractor essence jacquard weave medicine 3 times, sufficiently
Collect pollen；With kraft envelope dispense pollen, 10g/ bags.After sealing, every 10 packet is placed in the modeling equipped with 100g silica-gel desiccant
In envelope；By the pollen after packaging, it is protected from light and is placed in -40 DEG C of ultra low temperature freezer.
Three: Ba Danmu pollen production method of embodiment
The garden Ba Danmu that the florescence is in no disease and pests harm is base of picking flowers, and fair weather 11:00-18:00 uses plucking shears
Cooperation picking bag picking will open balloon-like petal.It when picking flowers, is nipped off from bennet, flower aperture≤0.5cm of picking
Mesh bag yarn display.After every 30 minutes collect 1 time, quickly spreads in ventilation, cool place, is protected from light place, thickness 1.5cm is spread, to adopt
Take time to count, for 24 hours within complete de- anther.After the quick reagent removal of anther seperator, it is sieved 1 time, is stood with the sieve of 120 mesh
Sterilizing and drying is carried out using anther drying device, drying device is kept for 21 DEG C of temperature, relative humidity≤18%.It is put down in drying bed
1 layer of template is spread, anther thickness < 3mm is spread.It is in yellow to anther shell, pollen grain golden yellow is glossy, tack-free after for 24 hours
When, i.e. drying terminates；The pollen on template is swept into pallet with brush, using pollen extractor essence jacquard weave medicine 3 times, sufficiently
Collect pollen；With kraft envelope dispense pollen, 10g/ bags.After sealing, every 10 packet is placed in the modeling equipped with 100g silica-gel desiccant
In envelope；By the pollen after packaging, it is protected from light and is placed in -40 DEG C of ultra low temperature freezer.
Example IV: Ba Danmu pollen production method
The garden Ba Danmu that the florescence is in no disease and pests harm is base of picking flowers, and fair weather 11:00-18:00 uses plucking shears
Cooperation picking bag picking will open balloon-like petal.It when picking flowers, is nipped off from bennet, flower aperture≤0.5cm of picking
Mesh bag yarn display.After every 30 minutes collect 1 time, quickly spreads in ventilation, cool place, is protected from light place, thickness 1.5cm is spread, to adopt
Take time to count, for 24 hours within complete de- anther.After the quick reagent removal of anther seperator, it is sieved 1 time, is stood with the sieve of 120 mesh
Sterilizing and drying is carried out using anther drying device, drying device is kept for 21 DEG C of temperature, relative humidity≤18%.It is put down in drying bed
1 layer of template is spread, anther thickness < 4mm is spread.It is in yellow to anther shell, pollen grain golden yellow is glossy, tack-free after 26h
When, i.e. drying terminates；The pollen on template is swept into pallet with brush, using pollen extractor essence jacquard weave medicine 3 times, sufficiently
Collect pollen；With kraft envelope dispense pollen, 10g/ bags.After sealing, every 10 packet is placed in the modeling equipped with 100g silica-gel desiccant
In envelope；By the pollen after packaging, it is protected from light and is placed in -40 DEG C of ultra low temperature freezer.
Five: Ba Danmu pollen production method of embodiment
The garden Ba Danmu that the florescence is in no disease and pests harm is base of picking flowers, and fair weather 11:00-18:00 uses plucking shears
Cooperation picking bag picking will open balloon-like petal.It when picking flowers, is nipped off from bennet, flower aperture≤0.5cm of picking
Mesh bag yarn display.After every 30 minutes collect 1 time, quickly spreads in ventilation, cool place, is protected from light place, thickness 1.5cm is spread, to adopt
Take time to count, for 24 hours within complete de- anther.After the quick reagent removal of anther seperator, it is sieved 1 time, is stood with the sieve of 120 mesh
Sterilizing and drying is carried out using anther drying device, drying device is kept for 21 DEG C of temperature, relative humidity≤18%.It is put down in drying bed
1 layer of template is spread, anther thickness < 5mm is spread.It is in yellow to anther shell, pollen grain golden yellow is glossy, tack-free after 28h
When, i.e. drying terminates；The pollen on template is swept into pallet with brush, using pollen extractor essence jacquard weave medicine 3 times, sufficiently
Collect pollen；With kraft envelope dispense pollen, 10g/ bags.After sealing, every 10 packet is placed in the modeling equipped with 100g silica-gel desiccant
In envelope；By the pollen after packaging, it is protected from light and is placed in -40 DEG C of ultra low temperature freezer.
Seven: Ba Danmu pollen of embodiment produces applicability analysis
For the best public skill process for determining the production of Ba Danmu pollen, on the basis of above-mentioned series embodiment one to five, with
Germination rate is index, spreads thickness, dry room temperature, drying time progress experiment of single factor to anther.On this basis, with sense
Official's scoring and hardness carry out the experimental design of Three factors-levels according to Box-Behnken center combination method for response,
And carry out response surface analysis.
1, the method for inspection
(1) Ba Danmu pollen germination rate detects
Ba Danmu pollen produced in embodiment one to five is subjected to germination rate detection, steps are as follows for specific method:
Wherein, the culture medium prescription of pollen germination detection is sucrose 10%, agar 1%, and preparation is distilled water with water；Training
It must heat and be stirred evenly with glass rod during the basigamy system of supporting, 105 DEG C of sterilizing 0.5h of high temperature；- 40 DEG C of refrigerator storages of defrosting
Pollen, 37 DEG C of warm water defrosting 5min；By culture medium drop on glass slide, the uniform bullet of pollen is dipped on culture medium with cotton swab；It will
Slide is put into constant incubator.Moist, dark, 25 DEG C of culture 6-24h；The percentage of pollen germination is measured under the microscope
(× 100) add culture ware lid.Continuous micro regulating focusing when detection, detects whether each pollen tube extends under different focal length, generally
It is more than that pollen diameter 2 times or more is determined as own sprouting by pollen tube length.10 visuals field of measurement at random calculate mean value, qualification flower
Powder is averaged pollen germination rate should be >=50%.The calculation formula of the pollen germination percentage of use are as follows: pollen germination percentage=each visual field
Pollen frequence × 100% in pollen tube growth pollen number/visual field.
2, single factor experiment
(1) influence of the drying time to Ba Danmu pollen purity and germination rate
5 processing groups of this experimental setup, drying time of each processing group be respectively 20h, 22h on the same day, for 24 hours,
26h,28h.Under same treatment conditions, the flower of picking is quickly spread, and carries out sterilizing and drying using petal drying device；Fastly
After fast reagent removal, it is sieved with the sieve of 120 mesh；Dry chamber ventilated is protected from light, 25 DEG C of room temperature of holding, under relative humidity≤18% environment
Dry, anther spreads with a thickness of < 3mm, using pollen extractor essence jacquard weave medicine 3 times, sufficiently collection pollen；It is kept away after bag distribution packaging
Light is placed in -40 DEG C of ultra low temperature freezer.The measurement that germination rate and purity are carried out to it obtains following experimental result.It can be with by Fig. 9
Find out, with the increase of drying time, pollen germination takes the lead in gradually decreasing after rising.The pollen germination when drying time is for 24 hours
Rate highest.It can be seen that drying time influences significantly the germination rate of Ba Danmu pollen.Therefore, using germination rate as main indicator,
Comprehensively consider that production is practical, select 22h, for 24 hours, 26h puts down for the relatively excellent water of response surface experiments.
(2) influence of the dry room temperature to Ba Danmu pollen germination rate
5 processing groups of this experimental setup, the drying room temperature of each processing group is respectively 21 DEG C, 23 DEG C, 25 DEG C, 27 DEG C,
29℃.Under same treatment conditions, the flower of picking is quickly spread, and carries out sterilizing and drying using petal drying device；It is quickly de-
After medicine, it is sieved with the sieve of 120 mesh；Dry chamber ventilated is protected from light, and dry under relative humidity≤18% environment, anther spreads thickness
For < 3mm, drying for 24 hours, using pollen extractor essence jacquard weave medicine 3 times, sufficiently collects pollen；It is protected from light after bag distribution packaging and is placed in -40
DEG C ultra low temperature freezer.The measurement that germination rate and purity are carried out to it obtains following experimental result.As seen from Figure 10, with
The raising of dry room temperature, Ba Danmu pollen germination take the lead in gradually decreasing after rising.It is sprouted when dry room temperature is 25 DEG C
Rate highest.Therefore, using Ba Danmu pollen germination rate as main indicator, comprehensively consider production reality, select 23 DEG C, 25 DEG C, 27 DEG C
It is flat for the relatively excellent water of response surface experiments.
(3) anther spreads influence of the thickness to Ba Danmu pollen germination rate
5 processing groups of this experimental setup, the anther of each processing group spread thickness be respectively < 1mm, < 2mm, < 3mm,
< 4mm, < 5mm.Under same treatment conditions, the flower of picking is quickly spread, and carries out sterilizing dry using petal drying device
It is dry；After quick reagent removal, it is sieved with sieve；It dries chamber ventilated, be protected from light, dried under 25 DEG C of relative humidity of room temperature≤18% environment for 24 hours,
Using pollen extractor essence jacquard weave medicine 3 times, sufficiently collection pollen；It is protected from light after bag distribution packaging and is placed in -40 DEG C of ultra low temperature freezer.It is right
Its measurement for carrying out germination rate, obtains following experimental result.As seen from Figure 11, as anther spreads the raising of thickness, sense
Official's scoring first rises to be gradually decreased afterwards.The Ba Danmu pollen germination rate highest when anther spreads with a thickness of < 3mm.Therefore, with bar
Denier wood pollen germination rate is main indicator, comprehensively considers production reality, selecting < 2mm, < 3mm, < 4mm is response surface experiments
It is flat compared with excellent water.
3, response surface optimization is tested
On the basis of single factor experiment, selection drying time, dry room temperature, anther spread thickness and carry out Box-
Behnken center combination design simultaneously combines actual experiment condition to choose reasonable level, is worth in response with sensory evaluation scores and hardness
The response surface analysis experiment of Three factors-levels is devised, a combination thereof design level value and coding are shown in Table 1.
Table 1: factor level coding schedule
On the basis of experiment of single factor, according to Box-Behnken experimental design principle, drying time (A), drying are chosen
Room temperature (B), anther spread thickness (C) and carry out Three factors-levels response surface analysis, in response with germination rate (Y1) index
Value, experimental design scheme and the results are shown in Table 2.Regression analysis, response surface are carried out to data using 8.0 software of Design Expert
Result figure is detailed in Figure 12, Figure 13, Figure 14.
Table 2: response surface experiments scheme and result
Interpretation of result and the foundation of model equation are carried out according to the experiment of table 2.
The variance analysis of table 3:Y1 regression model
Note: P < 0.05 *, significant difference；P < 0.01 *, difference are extremely significant.
Regression fit is carried out to data in table 4, obtains the secondary multinomial regression equation of independent variable Yu germination rate (Y1) are as follows:
Y1=+74.41+2.52*A+2.57*B+3.22*C-3.47*A*B-4.62*A*C-4.46*B* C-7.25*A2-
7.89*B2-6.16*C2
The variance analysis further carried out to regression model, as seen from the results in Table 4, model p < 0.0001 shows to return mould
Type reaches extremely significant level, the significant (R of the linear relationship between dependent variable and all independents variable2=0.8904).Lose quasi- item p=
0.9868 > 0.05, it is not significant that quasi- item is lost in simulation；The correction coefficient of determination R of the regression model2Adj=0.7495 shows to respond
Value variation can use the model explanation.Coefficient of variation CV=0.688 illustrates that the models fitting degree is good, and test error is small, therefore should
Regression equation model is set up, and can carry out analysis and prediction to Ba Danmu pollen manufacture craft result with this model.
4, response surface optimization and verifying
It using Design-Expert software, is maximized by germination rate, simplified regression equation combine asking
Solution, the Optimizing Technical of obtained Ba Danmu pollen are shown in Table 4.
Table 4: response surface software prediction optimum process condition
| Drying time/h | Dry room temperature/DEG C | Anther spreads thickness/mm | Germination rate/% |
| 24.18 | 25.17 | 3.20 | 74.94 |
In view of the operability and simplicity during actual experiment, drying time is set to for 24 hours, dry room temperature is
It 25 DEG C, spreads with a thickness of < 3mm.In order to examine the accuracy and validity of acquired results, 3 have been carried out using above-mentioned Optimal Parameters
Secondary parallel laboratory test, acquired results are as shown in table 5.As can be seen from Table 5, experimental result value relatively connects with response surface theoretical expectation values
Closely, it is therefore contemplated that it is accurate and reliable based on the resulting optimization of C/C composites parameter of the Response Surface Method.
Table 5: best Ba Danmu pollen technological experiment result verification
| Response | 1 | 2 | 3 | Average value |
| Germination rate/% | 79.65 | 78.36 | 79.10 | 78.57 |
Seven: Ba Danmu flower picker of embodiment
Referring to Figure of description 2-4, hook 121 that the present invention uses, zipper 13, neckstrap 111, shoulder belt 112 and waistband
113, safety cock 26 can be bought or be customized by public channel.
Present invention simultaneously provides a kind of portable flower pickers, including pick basket 1a and plucking shears 2, in plucking shears 2,
Serrated knife blade 22 is fixedly installed in 23 one end of arc-shaped handle, and curved blade 21, arc-shaped hand is fixedly installed in 231 one end of close handles
Handle 23 and close handles 231 pass through fixed swivel bearing 24 hingedly, and mesh bag slot 25, the detachable string bag 27 are arranged in serrated knife blade 22
It is flexibly connected with mesh bag slot 25, connection ring 272 is arranged in detachable 27 side of the string bag；It picks in basket 1a, the basket back body 11a upper edge
Neckstrap 111 is set, and harness 112 is arranged in the basket two sides body 11a, and the basket bottom body 11a is arranged waistband 113, is equipped with hook inside basket body 11a
Array is equipped with hook 121 on plate 12a, hanger plate 12a, and hook 121 is adapted setting, the setting of the basket bottom end body 11a with connection ring 272
Zipper 13, two side lower part of basket body 11a are arranged kit bag 14, are equipped with the fixation being adapted with arc-shaped handle 23 on the outside of kit bag 14
Band 141.
In the present invention, on the detachable string bag 27 272 opposite side of connection ring be arranged T-type item 271,271 one end of T-type item with
The detachable string bag 27 is fixedly connected, and 271 other end of T-type item and mesh bag slot 25 are adapted setting.
In the present invention, 23 arc range of arc-shaped handle is set as between π -1.5 π, convenient for 14 outside of hanging tool bag.
In the present invention, close handles 231 meet the setting of manpower structure feature, are subject to and the finger of manpower four is facilitated to protrude into.
In the present invention, the rectangular array on hanger plate of hook 121 is arranged, and avoiding stacking excessively high causes petal to weigh wounded.
In the present invention, the up-narrow and down-wide trapezoidal shape of basket body 11a, prevents from toppling over, anti-influence sight, and basket body 11a is thick fiber crops
Fabric material, good permeability, prevent petal it is too fast here.
In the present invention, zipper 13 is detachable and interchangeable, is 15N by the carrying force upper limit, convenient for unloading, work efficiency is high.
In the present invention, tightness can be adjusted by buckle in shoulder belt 112, neckstrap 111 and waistband 113.
In the present invention, serrated knife blade 22 can precisely block petal root, improve picking efficiency.
In the present invention, safety cock 26, the rotatable setting of safety cock 26 is arranged in the outer end of serrated knife blade 22.It, can when operation
Prevent from the detachable string bag 27 from skidding off to fall off, when non-operation can card curved blade 21, prevent from stabbing staff.
In the present invention, bracket 110 is 15N by the carrying force upper limit, and shape can be adjusted flexibly, and overweight mutability shape can prevent
Only staff's aerial work, accidental falling are seriously stabbed.
Eight: Ba Danmu anther drying device of embodiment
Referring to Figure of description 5-8, the LED- bactericidal lamp 21 that the present invention uses, orientation pulley 34, motor 4, gear 1,
Gear 2 42, chain 43, blower 5 can be bought or be customized by public channel.
The present invention provides a kind of Ba Danmu anther dry separation sterilizing unit, including hopper 1, drying bed 2, transmission mechanism and
Blow tank 6, hopper 1 and 2 one end of drying bed are adapted by 12 fixed-link of ladder chute, 12 upper end of ladder chute and hopper 1
Setting, 12 lower end of ladder chute are fixedly connected with 2 one end of drying bed, and the other end and 6 fixed-link of blow tank of drying bed 2 are done
Dry bed 2 is obliquely installed, and LED- bactericidal lamp 21 is equipped with above drying bed 2,2 surrounding of drying bed and top are respectively provided with transparent glass cover；
The top of drying bed 2 is equipped with brush board 3, and the upper end of brush board 3 is equipped with connection ring 33, setting orientation pulley 34, rail on the crossbeam of brush board 3
Road 35 is located at fixed setting right above 2 two sides of drying bed, and orientation pulley 34 and track 35 are adapted setting, 35 formed plane of track
Gradient it is consistent with the gradient of drying bed 2；The bottom end of brush board 3 is arranged hairbrush 31, hairbrush 31 by electric lifting rod 32 with
3 active link of brush board；Air hose 51, one end and adjustable 5 phase of air-heater of temperature of air hose 51 is fixedly installed in the two sides of drying bed 2
Logical, the air hose 51 of 2 two sides of drying bed is equipped with air outlet 52, and blade is arranged on air outlet 52 towards being oppositely arranged in air outlet 52
53, blade 53 is linked by sharf 55 with 52 axis of air outlet, the other end and 54 active link of electric telescopic rod of air hose 51,
Electric telescopic rod 54 and 56 fixed-link of blade loose-leaf, blade loose-leaf 56 and 53 fixed-link of blade；The setting point in blow tank 6
Expect baffle 61；Transmission mechanism includes motor 4, connection ring 33, gear 1, gear 2 42 and chain 43, motor 4 and gear 1
It is axially connected, gear 1 passes through chain 43 and 2 42 active link of gear, chain 43 and 33 fixed-link of connection ring；Control plane
Plate 7 and the adjustable air-heater 5 of motor 4, temperature, LED- bactericidal lamp 21, electric telescopic rod 54, electric lifting rod 32 electrically connect respectively
It connects.
In the present invention, bolt 11 is arranged in the bottom end of hopper 1, and bolt 11 is in embedded setting.
In the present invention, the side that drying bed 2 connects hopper 1 is higher than the side that drying bed 2 connects blow tank 6, drying bed 2
Tilt angle be 15 ° to 20 °.
In the present invention, blow tank 6 is arc setting.
In the present invention, the setting parallel with 6 center line of blow tank of sub-material baffle 61.
In the present invention, the amplitude of accommodation of blade 53 is 120 °.
In the present invention, connection ring 33 is symmetrical arranged in 3 two sides of brush board, and connection ring 33 and chain 43 are adapted setting.?
In the present invention, the control of control panel 7 includes electric telescopic rod 54, electric lifting rod 32, LED- bactericidal lamp 21, motor 4 and temperature
The air blast mode of adjustable air-heater 5.
When separating sterilizing unit using a kind of Ba Danmu anther provided by the invention is dry, kept for 25-27 DEG C of room temperature, phase
To humidity≤18%, dry chamber ventilated is protected from light.LED- bactericidal lamp 21 is opened by control panel 7, while light-illuminating,
A large amount of anion can be generated and intersperse among space, eliminated the unusual smell to play smoke abatement and dust control, disinfection and sterilization is dispelled the multiple efficacies such as pollution；
Anther is put into hopper 1, regulating pin 11 controls blanking velocity, prevents windrow from blocking hopper 1.According to anther granule size, lead to
It crosses control panel 7 and controls electric lifting rod 32, electric lifting rod 32 adjusts hairbrush 31, makes 31 height of hairbrush and anther size granularity
It is adapted, in fact it is highly preferred that 1-1.5cm；Electric telescopic rod 54 is controlled by control panel 7, electric telescopic rod 54 is with movable vane piece loose-leaf
56,56 linkage vane 53 of blade loose-leaf, blade 53 is rotated around sharf 55, and the blade 53 made is perpendicular to air outlet 52；Pass through
Start motor 4 in control panel 7, motor 4 drives gear 2 42 to rotate with moving gear 1, gear 1 by chain 43, chain
Item 43 drives brush board 3 to do periodic motion on track 35 by connection ring 33, and hairbrush 31 shakeouts anther on drying bed 2；It is logical
The hot wind mode of the adjustable air-heater 5 of 7 start-up temperature of control panel is crossed, temperature is adjustable, and air-heater 5 conveys hot wind by air hose 51
To air outlet 52, through blade 53, hot air convection toasts anther, and hairbrush 31 gently stirs the anther spread, keeps anther heated equal
Even, when pollen grain is glossy in golden yellow, pollen partly falls off, and drying operation closes to an end；It is controlled by control panel 7 electronic
Telescopic rod 54, electric telescopic rod 54 are revolved with movable vane piece loose-leaf 56,56 linkage vane 53 of blade loose-leaf, blade 53 around sharf 55
Turning, makes blade 53 towards blow tank 6,2 two sides blade 53 of drying bed is mutually angled, and preferably 90 ° -120 °；It is opened by control panel 7
The cool wind mode of dynamic temperature is adjustable air-heater 5, cold wind form resultant force and filigree and pollen blow off drying bed 2, by effect of inertia,
Into blow tank 6, pollen quality is lighter than filigree, and the distance blown is farther, and filigree and other impurity quality are larger, the distance blown
Closely, the Centrifugal dispersion in the blow tank of arc 6, pollen envelop sub-material baffle 61 separate, complete Ba Danmu anther sterilizing and drying and
Ba Danmu pollen detached job.
Nine: Ba Danmu pollen purity detecting of embodiment
The Ba Danmu pollen for Ba Danmu pollen and the conventional method production that optimum process produces in selection example seven carries out
Purity detecting: it will uniformly be scattered in thickness on the glass slide of 0.8-1.0mm for inspection pollen, under an optical microscope (× 100)
Observe its form.Suitable view is selected, focal length is adjusted, form judgement is carried out to a particle in the visual field.Qualified POLLEN MORPHOLOGY is full
Full, for pollen wall without invagination, the pollen equatorial plane is that length is spherical, and germinal aperature the micro- prominent angle end in face or is held level with both hands together, tool three under the line with angle
Hole ditch, shallow and narrow at proximal pole plate, the nearly equatorial plane is wide and deep, and costa colpi is irregular.Qualified pollen purity should be 80% or more.It is spent for inspection
Powder field of view is not less than 10.The calculation formula of pollen purity are as follows: total number of particles in pollen purity=qualification pollen number/visual field ×
100%.
6 Ba Danmu pollen purity detecting result of table
As described above, the present invention can be realized preferably, the above embodiments are only to preferred implementation side of the invention
Formula is described, and is not intended to limit the scope of the present invention, and without departing from the spirit of the design of the present invention, this field is general
The various changes and improvement that logical technical staff makes technical solution of the present invention, should all fall into present invention determine that protection scope
It is interior.
Claims (9)
1. a kind of Ba Danmu pollen processing technology, it is characterised in that:
(1) flower acquires
It is base of picking flowers with the garden Ba Danmu that no disease and pests harm is in the florescence, Best Times of picking flowers are fair weather 11:00-18:00,
Picking will open balloon-like petal；When picking flowers, nipped off from bennet using flower picker, the flower of picking with aperture≤
The mesh bag yarn of 0.5cm is displayed；After every 30 minutes collect 1 time, quickly spreads in ventilation, cool place, is protected from light place, spread thickness 1.5cm；
(2) anther is detached from
Counted with florescence, for 24 hours within complete de- anther；The petal and petal obtained using anther seperator to step 1 is fast
After fast reagent removal, it is sieved 1 time with the sieve of 120 mesh, is immediately fed into drying device；
(3) anther is dry
Sterilizing and drying is carried out using anther drying device, drying device is kept for 25-27 DEG C of temperature, relative humidity≤18%；Drying bed
1 layer of template of interior tiling, spreads anther thickness 1-5mm；It is in yellow to anther shell after dry 20-26h, pollen grain golden yellow has
Gloss, it is tack-free when, i.e., drying terminate；
(4) anther essence mentions
Pollen obtained in step 3 is swept into pallet with brush, using pollen extractor essence jacquard weave medicine 3 times, sufficiently collects flower
Powder；
(5) pollen is packed
With kraft envelope dispense pollen, 10g/ bags；After sealing, every 10 packet is placed in the plastic packaging bag equipped with 100g silica-gel desiccant
In；
(6) pollen storage
By the pollen after packaging, it is protected from light and is placed in -40 DEG C of ultra low temperature freezer.
2. a kind of Ba Danmu pollen processing technology as described in claim 1, which is characterized in that the hothouse room temperature 25
DEG C, anther thickness < 3mm is spread, pollen drying time is for 24 hours.
3. a kind of Ba Danmu pollen processing technology as claimed in claim 2, which is characterized in that it is described spread anther with a thickness of
3mm。
4. a kind of portable petal picker, including picking basket and plucking shears, which is characterized in that in plucking shears, arc-shaped hand
Serrated knife blade is fixedly installed in handle one end, and curved blade is fixedly installed in close handles one end, and arc-shaped handle passes through with close handles
Fixed swivel bearing is hinged, and mesh bag slot is arranged in serrated knife blade, and the detachable string bag is flexibly connected with mesh bag slot, the detachable string bag one
Connection ring is arranged in side；It picks in basket, basket carries portion upper edge setting neckstrap on the body, and harness is arranged in basket body two sides, and waist is arranged in basket body bottom
Band, basket body inside are equipped with hanger plate, and array is equipped with hook on hanger plate, and hook and connection ring are adapted setting, and basket body bottom end is set
Zipper is set, two side lower part of basket body is arranged kit bag, is equipped with the fixing belt being adapted with arc-shaped handle on the outside of kit bag.
5. a kind of portable petal picker as claimed in claim 4, which is characterized in that connect on the detachable string bag
Ring opposite side setting T-type item is connect, T-type one end is fixedly connected with the detachable string bag, and the T-type other end and mesh bag slot are adapted
Setting.
6. a kind of portable petal picker as claimed in claim 4, which is characterized in that the outer end of the serrated knife blade
Safety cock, the rotatable setting of safety cock are set.
7. a kind of dry separation sterilizing unit of Ba Danmu anther, which is characterized in that including hopper, drying bed, transmission mechanism and go out
Hopper, hopper and drying bed one end are adapted setting, trapezoidal cunning by ladder chute fixed-link, ladder chute upper end and hopper
One end fixed-link of slot lower end and drying bed, the other end and blow tank fixed-link of drying bed, drying bed are obliquely installed, do
LED- bactericidal lamp is equipped with above dry bed, drying bed surrounding and top are respectively provided with transparent glass cover；The top of drying bed is equipped with brush board,
Brush board upper end is equipped with connection ring, and setting orientation pulley on the crossbeam of brush board, track is located at fixed setting right above drying bed two sides,
The gradient of track formed plane and the gradient of drying bed are consistent, and orientation pulley and track are adapted setting；The bottom end of brush board
Hairbrush is set, and hairbrush passes through electric lifting rod and brush board active link；Drying bed two sides be fixedly installed air hose, the one of air hose
End is communicated with the adjustable air-heater of temperature, and the air hose of drying bed two sides is equipped with air outlet, and air outlet direction is oppositely arranged, air outlet
Upper setting blade, blade are linked by sharf with air outlet axis, the other end and electric telescopic rod active link of air hose, electricity
Dynamic telescopic rod and blade loose-leaf fixed-link, blade loose-leaf and blade fixed-link；Sub-material baffle is set in blow tank；Transmission
Mechanism includes motor, connection ring, gear one, gear two, chain, and motor is axially connected with gear one, gear one by chain with
Two active link of gear, chain and connection ring fixed-link；Control panel and the adjustable air-heater of motor, temperature, LED- bactericidal lamp,
Electric telescopic rod, electric lifting rod are electrically connected.
8. the dry separation sterilizing unit of a kind of Ba Danmu anther as claimed in claim 7, which is characterized in that below the blade
Equipped with gear, the amplitude of accommodation of blade is 120 °.
9. the dry separation sterilizing unit of a kind of Ba Danmu anther as claimed in claim 7, which is characterized in that drying bed binder
Side struggle against higher than drying bed connection blow tank side, tilt angle is 15 ° to 20 °.
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|---|---|---|---|
| CN201811508034.6A CN109496832A (en) | 2018-12-11 | 2018-12-11 | A kind of Ba Danmu pollen production method and its device of use |
| CN201911216439.7A CN110896847B (en) | 2018-12-11 | 2019-12-02 | Method for producing almond pollen and device adopted by same |
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| CN201811508034.6A CN109496832A (en) | 2018-12-11 | 2018-12-11 | A kind of Ba Danmu pollen production method and its device of use |
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| CN201811508034.6A Pending CN109496832A (en) | 2018-12-11 | 2018-12-11 | A kind of Ba Danmu pollen production method and its device of use |
| CN201911216439.7A Active CN110896847B (en) | 2018-12-11 | 2019-12-02 | Method for producing almond pollen and device adopted by same |
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| CN (2) | CN109496832A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111527884A (en) * | 2020-06-30 | 2020-08-14 | 四川和泽农业有限公司 | Method for preparing pollen for pollination |
| CN113455187A (en) * | 2021-06-03 | 2021-10-01 | 安徽农之源生态农业有限公司 | Pollen collecting and preserving method for prolonging pollen vitality |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6760621B2 (en) * | 2016-02-16 | 2020-09-23 | 香川県 | Pollen collector |
| CN105993400A (en) * | 2016-05-21 | 2016-10-12 | 新疆农业大学 | Method for rapidly preparing Amygdalus communis pollen |
| KR101831257B1 (en) * | 2016-07-12 | 2018-02-22 | 전남대학교 산학협력단 | Apparatus for separating and gethering the flower's male stamen |
| CN208012237U (en) * | 2018-01-27 | 2018-10-26 | 云南钰腾生物科技有限公司 | A kind of special drying box of pollen pini |
| CN108444274B (en) * | 2018-02-12 | 2019-11-15 | 中国农业科学院蜜蜂研究所 | A kind of Bee Pollen continuous drier and its drying means |
| CN108362092A (en) * | 2018-02-12 | 2018-08-03 | 中国农业科学院蜜蜂研究所 | A kind of Bee Pollen drying device and its drying means |
-
2018
- 2018-12-11 CN CN201811508034.6A patent/CN109496832A/en active Pending
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2019
- 2019-12-02 CN CN201911216439.7A patent/CN110896847B/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111527884A (en) * | 2020-06-30 | 2020-08-14 | 四川和泽农业有限公司 | Method for preparing pollen for pollination |
| CN113455187A (en) * | 2021-06-03 | 2021-10-01 | 安徽农之源生态农业有限公司 | Pollen collecting and preserving method for prolonging pollen vitality |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110896847B (en) | 2024-04-12 |
| CN110896847A (en) | 2020-03-24 |
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