CN109481726A - A kind of biodegradable hemostasis bone wax and preparation method thereof - Google Patents
A kind of biodegradable hemostasis bone wax and preparation method thereof Download PDFInfo
- Publication number
- CN109481726A CN109481726A CN201811418817.5A CN201811418817A CN109481726A CN 109481726 A CN109481726 A CN 109481726A CN 201811418817 A CN201811418817 A CN 201811418817A CN 109481726 A CN109481726 A CN 109481726A
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- China
- Prior art keywords
- bone wax
- hemostasis
- biodegradable
- keratin
- glycerol
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0042—Materials resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/108—Specific proteins or polypeptides not covered by groups A61L24/102 - A61L24/106
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Abstract
The present invention provides a kind of biodegradable hemostasis bone wax, its prepared composition is made of acetic acid, glycerol and keratin, the mass ratio of the acetic acid, glycerol and keratin are as follows: 1:1-3:8-10, preparation method is that keratin is added in acetic acid solution, it carries out continuing stirring in water bath with thermostatic control, then glycerol is added, then is stirred uniformly mixed.Hemostasis bone wax of the invention has degradability, excellent sealing characteristics and quick-acting haemostatic powder performance.
Description
Technical field
The present invention relates to hemostatic material technical fields, relate more specifically to a kind of hemostasis bone wax and its system that can give birth to solution degradation
Preparation Method.
Background technique
Bone wax is a kind of material that marrow portion capillary oozing of blood is blocked by physical method, can effectively block cancellous bone
Bleeding, the hemostasis that can be used for when various first-aid patients bone oozings of blood.The specific mechanism of action of bone wax is: one side bone wax is stopping blooding
There is very high absorption to liquid in the process, by absorbing the moisture in blood rapidly in the bleeding surface of a wound to promote blood platelet
Concentration, to enhance the agglutination speed and agglutinability of blood platelet；On the other hand, it is gluey that water-setting is formed after bone wax water suction, it can
It blocks in wound surface, increases the effect of hemostasis.In addition, can be formed to peripheral vessels has centainly after bone wax water swelling
The contention effect of pressure further promotes hemostasis.
Clinically present used bone wax, is mostly 70% beeswax and the mixture of 30% vaseline, has good
Softening performance, energy plastotype, nontoxic after rubbing softening with hand rubbing, but can not be degraded and absorb by body.Since beeswax and vaseline are mixed
The bone wax material for closing object preparation is unable to organism absorption, leaves the probability that will increase infection as foreign matter in vivo.Simultaneously as point
Sub- gap is too small, bone tissue can not by bone wax material, can hindering bone growth, it is postoperative will appear that wound do not recover for a long time ask
Topic, needs to carry out postoperative debridement when necessary.Treatment time is not only extended in this way, also increases the pain of patient.
Therefore it develops that a kind of haemostatic effect is good and the bone wax material of degradable absorption, while bone wax being made to can satisfy clinic
The mechanical property requirements used have great importance and are worth.
Summary of the invention
The purpose of the present invention is being absorbed for bone wax non-degradable in the prior art, leaves and be unfavorable for wound healing in vivo
The defects of, a kind of biodegradable hemostasis bone wax is provided.The present invention passes through the coordinated using acetic acid, glycerol and keratin
Effect, not only there is excellent physics to block, clog performance and haemostatic effect for the bone wax product being prepared, but also can give birth to completely
Object degradation absorbs, while product has excellent mechanical performance.
In order to solve the above technical problems, the technical scheme adopted by the invention is that:
A kind of biodegradable hemostasis bone wax, prepared composition are made of acetic acid, glycerol and keratin, the acetic acid,
The mass ratio of glycerol and keratin are as follows: 1:1-3:8-10.
In mentioned component, acetic acid plays solubilising, and glycerol and keratin, which cooperate, generates excellent closure hemostasis effect
Fruit.
In above-mentioned mass ratio, if glycerol less than 1, cannot generate good haemostatic effect, if carbomer gel is greater than
3, then biological degradability is poor.
In above-mentioned mass ratio, if keratin less than 8, cannot generate good plugging effect, if unsaturated polyester amide
Greater than 10, then it is unfavorable for the growth of newborn bony structures.
Effective plugging material when bone wax is for cancellous bone bleeding, traditional material are the mixture of paraffin and beeswax, body
Interior non-degradable, therefore will limit the growth of newborn bony structures.Hemostasis bone wax of the invention is by using biodegradable suction
Acetic acid, glycerol and the keratin of receipts are made, and block with excellent physics, clog performance and haemostatic effect, and can give birth to completely
Object degradation absorbs, and without limitation on the growth of newborn bony structures, while product has excellent mechanical performance.
The present invention also provides a kind of preparation methods of biodegradable hemostasis bone wax comprising following steps:
S1. keratin is added in acetic acid solution, carries out continuing stirring in water bath with thermostatic control；
S2. glycerol is added, then is stirred uniformly mixed.
Preferably, the temperature of water bath with thermostatic control is 30-40 DEG C.
Preferably, mixing time is 15-30min in step S1.
Preferably, mixing time is 10-15min in step S2.
Preferably, preparation method further includes that step S3 carries out irradiation sterilization to obtained hemostasis bone wax.
Compared with prior art, the beneficial effects of the present invention are:
The present invention provides a kind of biodegradable hemostasis bone wax and preparation method thereof, and the bone wax that stops blooding is by acetic acid, glycerol
It is formed with keratin, has the function of degradability and excellent physical blocks and quick-acting haemostatic powder performance.Hemostasis bone provided by the invention
The preparation method of wax is that keratin is added in acetic acid solution, carries out continuing stirring in water bath with thermostatic control, glycerol is then added, then
It is stirred uniformly mixed.Hemostasis bone wax ingredient of the invention is simple, and preparation process is simple, and it is suitable that the hemostasis bone wax of preparation has
Volume sweell(ing) rate and saturated water absorption, the degradation time of bone wax be 8-15 week, burst pressure is not less than 80mmHg, with good
Good plugging effect can be widely applied to orthopaedics hemostasis field.
Specific embodiment
The present invention is further illustrated With reference to embodiment, but embodiment the present invention is not done it is any
The restriction of form.
Embodiment 1
A kind of biodegradable hemostasis bone wax, prepared composition are made of acetic acid, glycerol and keratin, the acetic acid,
The mass ratio of glycerol and keratin are as follows: 1:1:8.
The hemostasis bone wax the following steps are included: keratin is added in S1. in acetic acid solution, in 30 DEG C of waters bath with thermostatic control into
Row persistently stirs 30min；S2. glycerol is added, is stirred for 10min and is uniformly mixed.
Embodiment 2
A kind of biodegradable hemostasis bone wax, prepared composition are made of acetic acid, glycerol and keratin, the acetic acid,
The mass ratio of glycerol and keratin are as follows: 1:2:9.
The hemostasis bone wax the following steps are included: keratin is added in S1. in acetic acid solution, in 40 DEG C of waters bath with thermostatic control into
Row persistently stirs 30min；S2. glycerol is added, is stirred for 15min and is uniformly mixed.
Embodiment 3
A kind of biodegradable hemostasis bone wax, prepared composition are made of acetic acid, glycerol and keratin, the acetic acid,
The mass ratio of glycerol and keratin are as follows: 1:3:10.
The hemostasis bone wax the following steps are included: keratin is added in S1. in acetic acid solution, in 30 DEG C of waters bath with thermostatic control into
Row persistently stirs 40min；S2. glycerol is added, is stirred for 12min and is uniformly mixed.
Embodiment 4
A kind of biodegradable hemostasis bone wax, prepared composition are made of acetic acid, glycerol and keratin, the acetic acid,
The mass ratio of glycerol and keratin are as follows: 1:1:10.
The hemostasis bone wax the following steps are included: keratin is added in S1. in acetic acid solution, in 30 DEG C of waters bath with thermostatic control into
Row persistently stirs 30min；S2. glycerol is added, is stirred for 15min and is uniformly mixed.
The hemostasis bone wax as made from embodiment 1-4 can be applied directly to the enterprising whereabouts blood of the surface of a wound of bleeding, can also add
Enter after a small amount of water is mixed and uses.Hemostasis bone wax is soft in this way and can carry out moulding as needed, can play better filling
And plugging effect.
Comparative example 1
A kind of biodegradable hemostasis bone wax, component are made of acetic acid and keratin, remaining is the same as embodiment 1.
Comparative example 2
A kind of biodegradable hemostasis bone wax, component are made of ethyl alcohol, glycerol and keratin, remaining is the same as embodiment 1.
Comparative example 3
A kind of biodegradable hemostasis bone wax, component are made of acetic acid, glycerol and keratin, the ethyl alcohol, glycerol
With the mass ratio of keratin are as follows: 1:5:5, remaining is the same as embodiment 1.
Performance test
(1) resistance to compression explosion bulge test
External resistance to compression explosion bulge test experimental method: pressure test method is used, the thickness with multi-pore structure is specifically prepared
Degree is the acrylic plectane of 1cm, and plectane closed can be fixed on pressure tester port, and pressure tester is that there are plectane interfaces
Acrylic box, the opposite connection pressure-detecting device and device for exerting of plectane interface, after closed acrylic box connects plectane,
Hemostasis bone wax material of the invention is smeared in plectane aperture position, material will fill up entire hole, and plectane direction is downward, in box
Water is filled with, press and detects leakage numerical value.
External resistance to compression explosion bulge test is carried out to the hemostasis bone wax that embodiment 1-4 and comparative example 1-3 is obtained, comparative example 1 obtains
The burst pressure obtained is lower than 50mmHg, and the burst pressure that comparative example 2-3 is obtained is lower than 60mmHg, and embodiment 1-3 acquisition is quick-fried
Broken pressure is all larger than 80mmHg.
(2) cellulotoxic experiment
Culture cell is contacted by leaching liquor, using L929 as experimental cell, embodiment 1-4 is evaluated in cell proliferation observation
Toxic effect of the prepared bone wax to cell in vitro.The experimental results showed that hemostasis bone wax of the invention has good safety
Property, it has no toxic side effect to cell growth.
(3) validity experiment is blocked
By embodiment 1-4 resulting materials by external resistance to compression explosion bulge test experiments have shown that it blocks validity, specific experiment
Method is: preparing the acrylic plectane with a thickness of 1cm with multi-pore structure, plectane closed can be fixed on pressure tester end
Mouthful, pressure tester is that there are the acrylic box of plectane interface, the opposite connection pressure-detecting devices and pressure of plectane interface
Device smears this material in plectane aperture position after closed acrylic box connects plectane, and material will fill up entire hole, circle
Plate direction is downward, and water is filled in box, presses and detects leakage numerical value.The experimental results showed that the equal energy of embodiment 1-4 resulting materials
It is enough effectively blocked, the sealing characteristics of comparative example 1-3 is poor.
(4) hemostasis validity experiment
After experimental dog is anaesthetized, using normal sterile surgical procedure, dog hind leg skin is cut, manufactures bone defect bleeding.It is real
It tests a group hemostasis bone wax of use embodiment 1-3 to stop blooding, as a result can effectively stop blooding in 15 seconds, and existing without oozing of blood
As haemostatic effect is significant.And comparative example 1 can not effectively be stopped blooding, comparative example 2 needs 40 seconds, and comparative example 3 then needs 30 seconds
Clock.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (6)
1. a kind of biodegradable hemostasis bone wax, which is characterized in that its prepared composition is made of acetic acid, glycerol and keratin,
The mass ratio of the acetic acid, glycerol and keratin are as follows: 1:1-3:8-10.
2. the preparation method of biodegradable hemostasis bone wax according to claim 1, which is characterized in that including following step
It is rapid:
S1. keratin is added in acetic acid solution, carries out continuing stirring in water bath with thermostatic control；
S2. glycerol is added, then is stirred uniformly mixed.
3. the preparation method of biodegradable hemostasis bone wax according to claim 2, which is characterized in that water bath with thermostatic control
Temperature is 30-40 DEG C.
4. the preparation method of biodegradable hemostasis bone wax according to claim 2, which is characterized in that stirred in step S1
Time is 15-30min.
5. the preparation method of biodegradable hemostasis bone wax according to claim 2, which is characterized in that it further includes step
S3 carries out irradiation sterilization to obtained hemostasis bone wax.
6. the preparation method of biodegradable hemostasis bone % wax according to claim 2, which is characterized in that stirred in step S2
Mixing the time is 10-15min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811418817.5A CN109481726A (en) | 2018-11-26 | 2018-11-26 | A kind of biodegradable hemostasis bone wax and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811418817.5A CN109481726A (en) | 2018-11-26 | 2018-11-26 | A kind of biodegradable hemostasis bone wax and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109481726A true CN109481726A (en) | 2019-03-19 |
Family
ID=65696712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811418817.5A Withdrawn CN109481726A (en) | 2018-11-26 | 2018-11-26 | A kind of biodegradable hemostasis bone wax and preparation method thereof |
Country Status (1)
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| CN (1) | CN109481726A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114470307A (en) * | 2022-04-06 | 2022-05-13 | 天新福（北京）医疗器材股份有限公司 | Degradable hemostatic plugging adhesive and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114470307A (en) * | 2022-04-06 | 2022-05-13 | 天新福（北京）医疗器材股份有限公司 | Degradable hemostatic plugging adhesive and preparation method thereof |
| CN114470307B (en) * | 2022-04-06 | 2022-06-24 | 天新福（北京）医疗器材股份有限公司 | Degradable hemostatic plugging adhesive and preparation method thereof |
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