CN109481509A - Broad-spectrum antibacterial composition and application thereof - Google Patents
Broad-spectrum antibacterial composition and application thereof Download PDFInfo
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Abstract
Broad-spectrum antibacterial composition and application thereof, the invention discloses the effect of the broad-spectrum antibacterial of the composition based on myrobalan, can effectively inhibit simultaneously staphylococcus aureus, Streptococcusagalactiae, pseudomonad, Salmonella choleraesuls, Escherichia coli, A race beta hemolytic streptococcus, Streptococcus suis, bordetella bacilli, killing property bar formula bacillus, intestines poison salmonella, staphylococcus haemolyticus, enterococcus faecalis, in erysipelas bacillus one or more of bacteriums growth.
Description
Technical field
The invention belongs to field of Chinese medicinal composition, specifically relate to a kind of medical composition and its use based on myrobalan.
Background technique
China's livestock-raising scale constantly expands, and development prospect is good, lays a good foundation, plays for China's economic development
Huge facilitation.But various animals can all generate some diseases in development process, seriously affect the health of animal.
If not taking effective precautionary measures, some diseases can be spread rapidly, cause farm (family) by huge economic loss,
And seriously affect the development of animal husbandry and the expansion of scale.
Mammitis, also known as acute mastitis, milk is yellow, milk is swollen, is to be condensed in breast due to hemostasis poison gas and become carbuncle swells, breast occurs
Firmly, it swells, heat, pain, infected cattle refuses cub sucking or a kind of disease of hand milking, is more common in the cow of postpartum breastfeeding phase, especially
Be lactation period milk cow it is more common.According to statistics, mammitis accounts for 21%~23% that milk cow always falls ill.Because under milk amount caused by it
Drop, disease cream is discarded, or even since the suppuration of newborn area, gangrene, atrophy are so that permanently lose milking capacity, the output of milk is made to decline to a great extent,
Milk cow is caused to be eliminated.Mastitis for milk cows is that milk cow is most common, is also to endanger a kind of maximum disease to dairy production.Due to
China is in dairy high-speed development period, and the relevant technologies, measure and facility do not match also and perfect, and therefore, mammitis is made
At loss with regard to more serious, the 9%~10% of the total mortality of milk cow Zhan being eliminated every year by mammitis, annual economy
Loss is up to over ten billion Yuan, has given a heavy blow to the development of Chinese dairy.
In addition, the diseases such as livestock diarrhea, porcine respiratory disease also often occur, cause the livestocks such as milk cow, pig appetite stimulator,
Cough, expiratory dyspnea, in addition it is dead busy.Also, these diseases over the course for the treatment of again and again, especially send out by porcine respiratory disease
Sick rate is high, it is easy to develop as chronic disease, be often difficult to cure, seriously threaten animal health, bring larger economy to aquaculture
Risk.
Currently, having some symptomatic drugs for above-mentioned livestock disease, but usually pathogenic factor is complex, sometimes simultaneously
It is not a kind of simple disease to germ, morbidity is often associated with a variety of disease symptoms, therefore in only a kind of effect of selection
There can be certain careless omission on drug, cannot timely and effectively suit the medicine to the illness and cure the disease.
Broad-spectrum antibacterial refers to that antibacterial material and product reply multiple-microorganism have fungistatic effect.This kind of bacteriostatic agent is antibacterial
In extensive range, antimicrobial spectrum is big, referred to as broad-spectrum antibacterial agent.Such as: tetracycline and chloramphenicol, they are not only to gram-positive bacterium
There is bacteriostasis with gramnegative bacterium, and chlamydia, mycoplasma pneumoniae, Richettsia and certain protozoons have inhibition to make
With.Using broad-spectrum antibacterial agent, to various bacteria while inhibiting effect can be carried out in the case where only using a kind of bacteriostatic agent, side
Just effectively.
Therefore, it for a variety of pathogenic bacteria bacteriums of a variety of diseases of livestock, studies one kind and effectively inhibits various pathogens
Product, it is easy timely and effectively to alleviate or cure the correlations such as livestock mammitis, diarrhea, porcine respiratory using a kind of drug products
Disease is of great significance, and related disease is of great significance.
Summary of the invention
It finding in production practice, respiratory diseases in pigs, which occurs, for pig is mainly influenced by following several bacteriums, be respectively:
A race beta hemolytic streptococcus, haemophilus parasuis, Streptococcus suis, bordetella bacilli, killing property bar formula bacillus and these types thin more
The influence of bacterium mixed infection；Livestock mammitis is mainly influenced by following several bacteriums, is respectively: staphylococcus aureus, nothing
The influence of streptococcus lactis, pseudomonad and intestines toxicity Escherichia coli and these types of bacteria mixed infection；Diarrhea mainly by
The influence of several bacteriums below is respectively: Salmonella choleraesuls and intestines toxicity Escherichia coli and both bacteriums are mixed
The influence of conjunction.Emblic, younghusband jerusalemsage root, in spiny meconopsis herb it is one or more of with scold molecular composition to A race beta hemolysis
Property streptococcus, haemophilus parasuis, Streptococcus suis, bordetella bacilli, killing property bar formula bacillus and these types of bacterium more Mixed Microbes,
With, the Mixed Microbes of Streptococcusagalactiae, pseudomonad and intestines toxicity Escherichia coli and these types of bacterium, and, hog cholera is husky
The bacteriostasis of door Salmonella and intestines toxicity Escherichia coli and the Mixed Microbes of both bacteriums has not been reported.
The purpose of the present invention is to provide a kind of broad-spectrum antibacterial composition, using composition to leading to respiratory diseases in pigs disease
Inhibition (or killing) effect of the Related Bacteria of disease, livestock mammitis and diarrhea, and preparation is used for for preventing and/or controlling
Treat the Related product of diarrhea and/or mammitis.
The present invention provides a kind of composition, and raw material includes the component of following parts by weight meter: in younghusband jerusalemsage root, spiny meconopsis herb
1.6~2.2 parts of one kind, 1 part of emblic, 4~4.5 parts of myrobalan.
Further, raw material includes 1.9 parts of spiny meconopsis herb, 1 part of emblic, 4.2 parts of myrobalan；Or its raw material includes crab
2 parts of crab first, 1 part of emblic, 4.2 parts of myrobalan.
The present invention provides the preparation methods of above-mentioned composition, it includes following content:
(1) bulk pharmaceutical chemicals are taken by weight；
(2) take the water extract of emblic, myrobalan, the alcohol extract of younghusband jerusalemsage root, spiny meconopsis herb prepares product to obtain the final product.
The present invention also provides a kind of composition, the components including following parts by weight meter: younghusband jerusalemsage root extract, spiny meconopsis herb
1.5~2.2 parts of one of extract, 1 part of emblic extract, 5.5~6.5 parts of Fructus Chebulae extract.
Further, including 1.7 parts of spiny meconopsis herb extract, 1 part of emblic extract, 6 parts of Fructus Chebulae extract；Or packet
Include 2 parts of younghusband jerusalemsage root extract, 1 part of emblic extract, 6 parts of Fructus Chebulae extract.
Raw material myrobalan, emblic, younghusband jerusalemsage root and spiny meconopsis herb used in the present invention, can be used its medicinal powder,
Its extract can be used, the extract includes water extract and extractive with organic solvent.
Extractive with organic solvent, which refers to, carries out dissolution extraction using active constituent of the organic solvent to raw material, is concentrated and is formed
Extract；Water extract refers to that making solvent using water carries out dissolution extraction to the active constituent of raw material, concentration and what is formed mention
Take object.The extractive with organic solvent includes but is not limited to that methanolic extract, ethanol extract, n-butanol extract, ether mention
Take object, ethyl acetate extract, ligroin extraction, chloroform extract；The water extract includes but is not limited to neutrality
Water extract, acid water extract, alkaline water extract, salting liquid extract.
In embodiments of the present invention, drug of participating in the experiment is the extract of raw material.Wherein emblic, myrobalan are water extract, crab
Crab first, spiny meconopsis herb is alcohol extract.
The present invention also provides the bacteriostatic applications of above-mentioned composition.
The present invention also provides above-mentioned compositions in the product of preparation treatment and/or prevention bacterial infection related disease
Purposes.
Further, the bacterium includes staphylococcus aureus, Streptococcusagalactiae, pseudomonad, hog cholera Salmonella
Bacterium, Escherichia coli, A race beta hemolytic streptococcus, Streptococcus suis, bordetella bacilli, killing property bar formula bacillus, intestines poison sramana more
One of Salmonella, staphylococcus haemolyticus, enterococcus faecalis, erysipelas bacillus or multiple combinations.
The present invention also provides above-mentioned compositions in the product for preparing treatment livestock mammitis, diarrhea or porcine respiratory disease
In purposes.
Further, the diarrhea is bacterial diarrhea；Further, the bacterium include Salmonella choleraesuls, it is big
One of intestines escherich's bacillus or multiple combinations；
Further, the respiratory diseases in pigs is bacillary respiratory diseases in pigs；Further, the bacterium
Including A race beta hemolytic streptococcus, haemophilus parasuis, Streptococcus suis, bordetella bacilli, one of killing property bar formula bacillus more
Or multiple combinations；
Further, the mammitis is bacillary mammitis；Further, the pathogenic bacteria of the bacillary mammitis
Selected from one of staphylococcus aureus, Streptococcusagalactiae, pseudomonad, Escherichia coli or multiple combinations.
The beneficial effects of the present invention are: the present invention provides a series of broad-spectrum antibacterial purposes of compositions and they making
Application in standby related antibacterial product, the composition can effectively inhibit staphylococcus aureus, Streptococcusagalactiae, false unit cell
Bacterium, Salmonella choleraesuls, Escherichia coli, A race beta hemolytic streptococcus, Streptococcus suis, bordetella bacilli, killing property bar more
The growth of formula bacillus, intestines poison salmonella, staphylococcus haemolyticus, enterococcus faecalis, erysipelas bacillus has good wide spectrum application
Range, and be experimentally confirmed the validity of such use, safety is without side-effects, and broad spectrum activity is good, be respiratory diseases in pigs,
The treatment of livestock mammitis and diarrhea develops new drug approach.
Specific embodiment
Application of the present invention is described further below by specific embodiment and specific experiment, to the myrobalan or
The bacteriostasis of the composition series of its extract is verified and is illustrated.
Experimental material: myrobalan, emblic, younghusband jerusalemsage root, spiny meconopsis herb are purchased from southwestern Chinese herbal medicine wholesale market；Tested bacteria
Staphylococcus aureus, Streptococcusagalactiae, pseudomonad, Salmonella choleraesuls, intestines toxicity Escherichia coli (ETEC), A
Race beta hemolytic streptococcus, haemophilus parasuis, bordetella bacilli, more killing property bar formula bacillus are purchased from Chinese animal doctor's supervision institute, pig
Streptococcus is pig farm isolates and purifies and obtains.
One, the preparation of Fructus Chebulae extract
100g myrobalan's powder is weighed, after being wrapped with filter paper, is put into Soxhlet extractor, neutral deionized water Soxhlet is added and mentions
5h is taken, water extract is recycled, simultaneously constant volume is spare to get myrobalan's water extract for concentration on water-bath.
100g myrobalan's powder is weighed, after being wrapped with filter paper, is put into Soxhlet extractor, dehydrated alcohol Soxhlet extraction is added
6h recycles alcohol extracting thing, and simultaneously constant volume is spare to get myrobalan's ethanol extract for concentration on water-bath.
Two, the preparation of emblic extract
100g Phyllanfhus Fruit is weighed, after being wrapped with filter paper, is put into Soxhlet extractor, neutral deionized water Soxhlet is added
5h is extracted, water extract is recycled, is concentrated on water-bath and constant volume is to get emblic water extract.
100g Phyllanfhus Fruit is weighed, after being wrapped with filter paper, is put into Soxhlet extractor, dehydrated alcohol Soxhlet extraction is added
6h recycles alcohol extracting thing, and simultaneously constant volume is spare to get emblic ethanol extract for concentration on water-bath.
Three, the preparation of younghusband jerusalemsage root extract
100g younghusband jerusalemsage root powder is weighed, after being wrapped with filter paper, is put into Soxhlet extractor, neutral deionized water Soxhlet is added
5h is extracted, water extract is recycled, is concentrated on water-bath and constant volume is to get younghusband jerusalemsage root water extract.
100g younghusband jerusalemsage root powder is weighed, after being wrapped with filter paper, is put into Soxhlet extractor, dehydrated alcohol Soxhlet extraction is added
6h recycles alcohol extracting thing, and simultaneously constant volume is spare to get younghusband jerusalemsage root ethanol extract for concentration on water-bath.
Four, the preparation of spiny meconopsis herb extract
100g spiny meconopsis herb powder is weighed, after being wrapped with filter paper, is put into Soxhlet extractor, neutral deionized water is added
Soxhlet extraction 5h recycles water extract, is concentrated on water-bath and constant volume is to get spiny meconopsis herb water extract.
100g spiny meconopsis herb powder is weighed, after being wrapped with filter paper, is put into Soxhlet extractor, dehydrated alcohol Soxhlet is added
6h is extracted, alcohol extracting thing is recycled, simultaneously constant volume is spare to get spiny meconopsis herb ethanol extract for concentration on water-bath.
The yield of different extracts see the table below:
Above-mentioned yield can in 5% range normal fluctuation.
Embodiment 1
A kind of composition: by 1.9 parts of spiny meconopsis herb, 1 part and 4.2 parts of myrobalan of emblic is formed.
Embodiment 2
A kind of composition: by 2 parts of younghusband jerusalemsage root, 1 part and 4.2 parts of myrobalan of emblic is formed.
Embodiment 3
A kind of composition: by 1.7 parts of spiny meconopsis herb ethanol extract, 1 part of emblic water extract, myrobalan's water extract
6 parts of compositions.
Embodiment 4
A kind of composition: by 2 parts of younghusband jerusalemsage root ethanol extract, 1 part of emblic water extract, 6 parts of groups of myrobalan's water extract
At.
The measurement of 5 minimum inhibitory concentration of embodiment (MIC)
1. prepared by medicine storage liquid
It extracts drug and calculates the concentration of various extracts according to yield, and saved backup at 4 DEG C.
2. culture medium
Staphylococcus aureus, pseudomonad, Salmonella choleraesuls, intestines toxicity Escherichia coli, A race beta hemolysis
Property streptococcus, bordetella bacilli, killing property bar formula bacillus use caseinhydrolysate (MH) meat soup, pH=7.2~7.4；Secondary pig is bloodthirsty
Bacillus uses HTM meat soup；Streptococcusagalactiae, Streptococcus suis use the MH meat soup containing 2%~5% dissolution horse blood.
3. the preparation of inoculum
The form for choosing activation with oese is bacterium colony 3~5 to be checked similar, is inoculated in caseinhydrolysate (MH) meat of 4~5ml
Tang Zhong, 35 DEG C of 2~6h of incubation.Logarithmic growth phase bacterium solution physiological saline or MH meat soup corrected concentrations after increasing bacterium is to 0.5 Maxwell
Than turbid standard, containing about 1~2 × 108CFU/ml.With MH meat soup by above-mentioned bacteria suspension carry out 1: 100 dilution after it is spare.In 15min
It is inscribed to finish prepared inoculum, and a inoculum secondary culture on non-selective agar plate is taken, to check inoculation
Object purity.
4. diluting the preparation and bacterium solution inoculation of antibacterials
Sterile test tube (13 × 100mm) 9 is taken, is arranged in a row, in addition to 1.5ml MH meat soup is added in the 1st pipe, remaining every pipe
MH meat soup 1ml is added, antibacterials stoste 0.5ml is added in the 1st pipe and mixes, then draws 1ml to the 2nd and manages, inhaled again after mixing
1ml to the 3rd is taken to manage, so continuous doubling dilution to the 8th pipe, and draw 1ml from the 8th pipe and discard, the 9th pipe is not drug containing
Growth control.Then above-mentioned each 1ml of the inoculum prepared is added in every pipe, make the final bacterial concentration of every pipe be about 5 ×
105CFU/ml。
5. being incubated for
Inoculated dilution tube is stoppered plug, sets in 35 DEG C of normal air incubators and is incubated for 20h；Haemophilus and streptococcus
It is incubated for for 24 hours in normal air incubator.
6. the measurement of minimum inhibitory concentration (MIC)
Before reading and reporting the minimum inhibitory concentration of institute's test strain, check that the bacterial growth situation of growth control pipe is
It is no good, at the same also to check the secondary culture situation of inoculum to determine if to pollute, Quality-control strains it is minimum antibacterial dense
Whether angle value is in Quality Control range.To visually observe, drug minimum concentration pipe is without bacterial growth person, the minimum suppression of as tested bacterium
Bacteria concentration.Experimental result is shown in Tables 1 and 2s.
15-1 is expressed as myrobalan's water extract in table, and 5-1 is expressed as emblic water extract, and it is green that 4-2 is expressed as thorniness
Suede wormwood artemisia ethanol extract, 6-2 are expressed as younghusband jerusalemsage root ethanol extract, and the extract quality in table is respectively participated in the experiment than expression in drug
The extract quality concentration ratio (namely each component mass ratio in composition) of corresponding drug, bulk pharmaceutical chemicals mass ratio are according to extract
Yield and the mass ratio of required bulk pharmaceutical chemicals that calculates.Table 1 corresponds to table for drug number concentration of respectively participating in the experiment, and table 2 is medicine of respectively participating in the experiment
Object minimum inhibitory concentration Score Lists.Drug of respectively participating in the experiment in table 2 is 1 Chinese medicine of table for the minimum inhibitory concentration goals for of different bacterium
The corresponding number of object concentration, such as: the composition of myrobalan's water extract and younghusband jerusalemsage root ethanol extract is to hog cholera Salmonella in table 2
The minimum inhibitory concentration of bacterium is scored at 3, then it represents that it is 3 pairs of number in table 1 to the minimum inhibitory concentration of Salmonella choleraesuls
(myrobalan's water extract concentration is 6.0645mg/mL, younghusband jerusalemsage root ethanol extract concentration to the concentration 6.0645/5.8939mg/mL answered
For 5.8939mg/mL), and so on, 0 point indicates no bacteriostasis.G indicates staphylococcus aureus, agalasisa hammer in table 2
The Mixed Microbes of bacterium, pseudomonad and intestines toxicity Escherichia coli, H indicate Salmonella choleraesuls and the uncommon bar of intestines toxicity large intestine angstrom
The Mixed Microbes of bacterium, one column of total score are scored at relative medicine to staphylococcus aureus, Streptococcusagalactiae, pseudomonad, pig
Cholera salmonella, intestines toxicity Escherichia coli, A race beta hemolytic streptococcus, haemophilus parasuis, Streptococcus suis, wave
The adduction of family name bacillus, more killing property bar formula bacillus and two groups of Mixed Microbes scores of G and H.Score is higher, drug of participating in the experiment needed for expression
Minimum inhibitory concentration is lower, on the contrary then anti-.
The drug number concentration of respectively participating in the experiment of table 1. corresponds to table
Table 2. is respectively participated in the experiment minimal inhibitory concentration of drug Score Lists
From the above results, in addition to the combination of myrobalan's water extract and younghusband jerusalemsage root ethanol extract, myrobalan's water extract
And its different components have good broad-spectrum antibacterial effect to above-mentioned bacterium, can inhibit the above-mentioned germy growth of institute simultaneously
Breeding.Especially when using myrobalan's water extract, emblic water extract, three groups of subassemblys of spiny meconopsis herb ethanol extract or
When three groups of myrobalan's water extract, emblic water extract, younghusband jerusalemsage root ethanol extract subassemblys, to staphylococcus aureus, nothing
Streptococcus lactis, pseudomonad, Salmonella choleraesuls, intestines toxicity Escherichia coli, A race beta hemolytic streptococcus, secondary pig
Haemophilus, Streptococcus suis, bordetella bacilli, killing property bar formula bacillus and Mixed Microbes definite effective more, and works as and use myrobalan's water
When extract or the two-component composition of myrobalan, there is no fungistatic effect to haemophilus parasuis therein.
Three when myrobalan's water extract, emblic water extract, spiny meconopsis herb ethanol extract mass ratio are 6: 1: 1.7
The highest scoring of components composition illustrates that its bacteriostasis is most strong, shows as that minimum inhibitory concentration is lower, only needs low concentration
Drug can be effectively antibacterial.When the spiny meconopsis herb ethanol extract in three component is changed into younghusband jerusalemsage root ethanol extract,
Fungistatic effect has a little reduction, and need to improve drug concentration could be effectively antibacterial.Likewise, myrobalan's water extract, myrobalan's water extract
The composition of object and emblic water extract, the composition of myrobalan's water extract and spiny meconopsis herb ethanol extract and myrobalan
Water extract and the composition score of younghusband jerusalemsage root ethanol extract are opposite lower, illustrate to want effective broad-spectrum antibacterial these drugs
Or composition needs relatively higher concentration.
It can also analyze and obtain from experimental data, as exclusive use myrobalan, or using the combination of myrobalan and emblic, or
It is not antibacterial to haemophilus parasuis using the combination of myrobalan and spiny meconopsis herb, or using the combination of myrobalan and younghusband jerusalemsage root
Effect, and work as three groups of myrobalan, emblic, multiple pseudo-ginseng subassemblys, or three groups of myrobalan, emblic, younghusband jerusalemsage root subassemblys
Afterwards, fungistatic effect just being produced to haemophilus parasuis, it is seen then that its effect is not the simple adduction of its bulk pharmaceutical chemicals effect, three
Three kinds of medicinal materials of component combine synergy, produce unexpected technical effect.
The fungistatic effect of 6 each component drug water extract of embodiment and alcohol extract compares.
It is to investigate target with antibacterials antibacterial circle diameter size using paper disk method | (size of inhibition zone can reflect test
Bacterium to measurement drug sensitivity, and with the medicine to test bacterium minimum inhibitory concentration (MIC) it is negatively correlated, i.e., inhibition zone is got over
Greatly, MIC is smaller), as a result it see the table below.
| Diameter cm | ||||
| Water extracts susceptibility | Spiny meconopsis herb | Emblic | Younghusband jerusalemsage root | Myrobalan |
| Salmonella choleraesuls | 0 | 1.1 | 0 | 1.3 |
| Intestines poison salmonella | 0 | 1.1 | 0 | 1.1 |
| Bordetella bacilli | 0 | 1.7 | 0 | 1.9 |
| Pasteurella multocida | 0.8 | 1.6 | 1.2 | 2 |
| Pseudomonad | 0 | 0 | 0 | 1.2 |
| Escherichia coli | 0 | 1.2 | 0 | 0.9 |
| Staphylococcus aureus | 0 | 1.2 | 0 | 1.1 |
| Staphylococcus haemolyticus | 0 | 1.5 | 0 | 1.5 |
| Haemophilus parasuis | 0 | 1.2 | 0 | 1.7 |
| Enterococcus faecalis | 0 | 1.6 | 0 | 1.5 |
| Erysipelas bacillus | 0 | 1.7 | 0 | 1 |
| Streptococcus | 0 | 0.9 | 0 | 0.9 |
| Alcohol extracting susceptibility | Spiny meconopsis herb | Emblic | Younghusband jerusalemsage root | Myrobalan |
| Salmonella choleraesuls | 1.1 | 0 | 1.1 | 0 |
| Intestines poison salmonella | 1.1 | 0 | 1.7 | 0 |
| Bordetella bacilli | 1.1 | 1.2 | 1.8 | 1.5 |
| Pasteurella multocida | 1.7 | 2 | 1.6 | 3 |
| Pseudomonad | 0.7 | 0 | 0.7 | 0 |
| Escherichia coli | 1.4 | 0.8 | 1.2 | 0 |
| Staphylococcus aureus | 1.6 | 1 | 1.2 | 0.8 |
| Staphylococcus haemolyticus | 1.4 | 1.6 | 1 | 1.5 |
| Haemophilus parasuis | 1.8 | 1.9 | 2.1 | 2.4 |
| Enterococcus faecalis | 0 | 1.7 | 0 | 1.7 |
| Erysipelas bacillus | 1 | 1.2 | 1.5 | 1.8 |
| Streptococcus | 0 | 1.7 | 0 | 0 |
In conclusion myrobalan of the present invention or its extract and combinations thereof are to pathogen staphylococcus aureus, agalasisa chain
Coccus, pseudomonad, Salmonella choleraesuls, intestines toxicity Escherichia coli, A race beta hemolytic streptococcus, secondary pig are bloodthirsty
Bacillus, Streptococcus suis, bordetella bacilli, more killing property bar formula bacillus and Mixed Microbes all show effective antibacterial action, have very
Good broad spectrum activity, can be used for the preparation of corresponding broad-spectrum antibacterial product.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright description is applied directly or indirectly in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (10)
1. a kind of composition, which is characterized in that its bulk pharmaceutical chemicals includes the component of following parts by weight meter: younghusband jerusalemsage root, spiny meconopsis herb
One of 1.6~2.2 parts, 1 part of emblic, 4~4.5 parts of myrobalan.
2. composition according to claim 1, which is characterized in that including 1.9 parts of spiny meconopsis herb, 1 part of emblic, myrobalan
4.2 part；Or including 2 parts of younghusband jerusalemsage root, 1 part of emblic, 4.2 parts of myrobalan.
3. the preparation method of composition as claimed in claim 1 or 2, it is characterised in that: it includes following content:
(1) bulk pharmaceutical chemicals are taken by weight；
(2) take the water extract of emblic, myrobalan, the alcohol extract of younghusband jerusalemsage root, spiny meconopsis herb prepares product to obtain the final product.
4. a kind of composition, which is characterized in that the component including following parts by weight meter: younghusband jerusalemsage root extract, spiny meconopsis herb mention
Take 1.5~2.2 parts of one of object, 1 part of emblic extract, 5.5~6.5 parts of Fructus Chebulae extract.
5. composition according to claim 3, which is characterized in that including 1.7 parts of spiny meconopsis herb extract, emblic is extracted
1 part of object, 6 parts of Fructus Chebulae extract；Or including 2 parts of younghusband jerusalemsage root extract, 1 part of emblic extract, 6 parts of Fructus Chebulae extract；Into one
Step ground, the emblic extract, Fructus Chebulae extract are water extract, and younghusband jerusalemsage root extract, spiny meconopsis herb extract are alcohol extracting
Object.
6. the bacteriostatic application of any one of Claims 1 to 5 composition.
7. any one of Claims 1 to 5 composition is in preparation treatment and/or the product of prevention bacterial infection related disease
In purposes.
8. the purposes of claim 6 or 7, it is characterised in that: the bacterium include staphylococcus aureus, Streptococcusagalactiae,
It is pseudomonad, Salmonella choleraesuls, Escherichia coli, A race beta hemolytic streptococcus, Streptococcus suis, bordetella bacilli, more
One of killing property bar formula bacillus, intestines poison salmonella, staphylococcus haemolyticus, enterococcus faecalis, erysipelas bacillus or multiple combinations.
9. any one of Claims 1 to 5 composition is in the production of preparation treatment livestock mammitis, diarrhea or porcine respiratory disease
Purposes in product.
10. the purposes stated according to claim 9, it is characterised in that: the diarrhea is bacterial diarrhea；Further, described thin
Bacterium bag includes one of Salmonella choleraesuls, Escherichia coli or multiple combinations；
Further, the respiratory diseases in pigs is bacillary respiratory diseases in pigs；Further, the bacterium includes
A race beta hemolytic streptococcus, haemophilus parasuis, Streptococcus suis, bordetella bacilli, one of killing property bar formula bacillus or more more
Kind combination；
Further, the mammitis is bacillary mammitis；Further, the pathogenic bacteria of the bacillary mammitis are selected from
One of staphylococcus aureus, Streptococcusagalactiae, pseudomonad, Escherichia coli or multiple combinations.
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2018
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| Title |
|---|
| 余雄: "《奶牛肉牛高效养殖新技术》", 31 July 2006, 中国农业出版社 * |
| 刘圆等: "《中国民族药物学概论》", 30 November 2007, 四川民族出版社 * |
| 姜丙华等: "《感染性疾病诊断与治疗》", 31 October 2008, 天津科学技术出版社 * |
| 席晓蓉: "余甘子抗菌作用的研究进展", 《中国民族民间医药》 * |
| 张汀等: "《科学高效养猪指南》", 30 June 2006, 河南科学技术出版社 * |
| 杜宝中等: "藏药螃蟹甲提取物体外抗菌作用研究", 《西藏大学学报》 * |
| 顾健: "《中国藏药：汉藏对照》", 31 October 2016, 民族出版社 * |
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