CN109463489A - A kind of rice bran protein tea beverage and preparation method thereof - Google Patents

A kind of rice bran protein tea beverage and preparation method thereof Download PDF

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Publication number
CN109463489A
CN109463489A CN201910026415.9A CN201910026415A CN109463489A CN 109463489 A CN109463489 A CN 109463489A CN 201910026415 A CN201910026415 A CN 201910026415A CN 109463489 A CN109463489 A CN 109463489A
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rice bran
bran protein
tea
powder
tea beverage
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CN109463489B (en
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吴伟
吴晓娟
李芳�
林亲录
林利忠
梅小弟
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Central South University of Forestry and Technology
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Central South University of Forestry and Technology
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/06Treating tea before extraction; Preparations produced thereby
    • A23F3/14Tea preparations, e.g. using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C11/00Milk substitutes, e.g. coffee whitener compositions
    • A23C11/02Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
    • A23C11/10Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
    • A23C11/103Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk

Abstract

A kind of rice bran protein tea beverage and preparation method thereof, which is made of the raw material of following weight percent: 3%~4.2% rice bran protein powder, 1%~1.5% instant tea powder, 1.5%~1.8% plant phosphatide, 0.2%~0.5% phytosterol, 2%~2.4% maltitol, 2%~3.6% oligoisomaltose, excess water.The invention also includes the preparation methods of the rice bran protein tea beverage.Rice bran protein tea beverage of the present invention makes tea beverage with rice bran protein lotion substitution milk, can meet the needs of lactose intolerant patient;Rich in prebiotic agents, phosphatide and sterol, has the function of adjusting intestinal flora balance, inhibits human body to the absorption of cholesterol and prevention cardiovascular disease.Preparation method of the present invention effectively reduces the probability that the polyphenol substance in tealeaves is directly contacted with rice bran protein, precipitating is avoided the formation of, rice bran protein is enable to substitute lactoprotein, soybean protein, has both retained nutritional ingredient in rice bran, the mouthfeel for not influencing tea beverage again, can be widely applied to liquid tea-drinking material products.

Description

A kind of rice bran protein tea beverage and preparation method thereof
Technical field
The invention belongs to beverage production fields, and in particular to a kind of rice bran protein tea beverage and preparation method thereof.
Background technique
Tea is one of beverage most popular in the world, possesses various health care functions, such as anti-oxidant, anticancer, antibacterial, Main active is tea polyphenols.Catechin is the main matter in tea polyphenols, wherein Epigallo-catechin gallate (EGCG) Account for about the 50%~80% of catechin, Epigallo-catechin gallate (EGCG) is recognized due to its high-content and excellent bioactivity To be most important ingredient in tea polyphenols.For a long time, people, which like tea to be added in milk, prepares milk tea, because milk can To improve the organoleptic attribute of tea.But 90% or more Asian has lactose intolerance, the milk tea of milk production easily causes cream Intolerant to disease crowd the adverse reactions such as abdominal distension, abdominal pain, diarrhea occur for sugar.In recent years, someone substitutes milk for making with vegetable protein Make milk tea, to meet the needs of lactose intolerant patient.
Rice bran protein is a kind of generally acknowledged quality plant albumen, and anaphylaxis is low, what amino acid composition was recommended with FAO/WHO The idealized model of amino acid composition is very close, and biological value is high, can be widely applied to all kinds of including infant food In food.And China's 14,000,000 tons of rice brans of annual about generation, rice bran protein resource are extremely abundant.But compared to cow's milk protein, Rice bran protein is lacking in terms of dissolubility and functional character.In addition, tea polyphenols, epi-nutgall in rice bran protein and tealeaves The contact of the polyphenol substances such as catechin and gallate can interact, and form aggregation and precipitating.Therefore, rice bran or rice bran egg It is white to be chiefly used in solid-state food, it is fresh to be used for liquid beverage product less.
107279390 A of CN discloses a kind of ferment rice bran milk tea, first by rice bran complex enzyme zymohydrolysis, saccharomycete and cream Acid bacteria fermentation, then compounded with skimmed milk power, this milk tea is a kind of solid making drinks, and technique is sufficiently complex.
104137889 B of CN discloses a kind of rice bran protein composite nutritious beverage and processing method, by grain germ powder and Soybean compounding, by extrusion stability, allotment, sterilization, deodorization, homogeneous and etc. completion, the invention filled up domestic rice bran egg The blank in white composite nutritious beverage market, but this method not can solve rice bran protein and cause with polyphenols interaction Beverage layering, flocculation, deposited phenomenon, thus be not suitable for the production of rice bran protein tea beverage.
Summary of the invention
The technical problem to be solved by the present invention is to overcome drawbacks described above of the existing technology, provide a kind of not only simultaneous Have the mellow mouthfeel of protein beverage and the unique perfume of tea beverage, also there is good healthcare function, the good rice bran of stability Albumen tea beverage.
The technical solution adopted by the present invention to solve the technical problems is as follows: a kind of rice bran protein tea beverage, by following heavy The raw material of amount percentage is made: rice bran protein powder 3%~4.2%, plant phosphatide 1.5%~1.8%, is planted at instant tea powder 1%~1.5% Object sterol 0.2%~0.5%, maltitol 2%~2.4%, oligoisomaltose 2%~3.6%, pure water 86.0%~90.3%.
Preferably, rice bran protein powder, instant tea powder, plant phosphatide, phytosterol, maltitol, oligoisomaltose are food Grade raw material.Lipidated protein >=80% of rice bran protein powder.Instant tea powder can be instant black tea powder, instant green tea powder or speed Molten dark green tea powder;Plant phosphatide can be soybean lecithin or corn phosphatide;Phytosterol can be cupreol or stigmasterol.
Rice bran protein tea beverage of the present invention is to can satisfy lactose intolerance with rice bran protein lotion substitution milk production milk tea The demand of disease patient;Second is that the beverage is rich in prebiotic agents, has the function of certain adjusting intestinal flora balance;Third is that The beverage is rich in phosphatide and sterol, can inhibit absorption of the human body to cholesterol to a certain extent, has and prevents cardiovascular disease The effect of disease.
The further technical problems to be solved of the present invention are to overcome drawbacks described above of the existing technology, provide a kind of behaviour Make the preparation method of simple beverage.
Further technical solution to be solved is as follows by the present invention: a kind of preparation method of rice bran protein tea beverage is provided, The following steps are included:
(1) preparation of rice bran protein nanoemulsions: 3%~4.2% rice bran protein powder is dissolved in 40%~42% pure water, 30~ 40 DEG C, be stirred to react 60~90 min under 100~200 r/min, dissolve it sufficiently.Then Microfluidizer pair is used Mixed solution carries out high-pressure homogeneous processing, 80~100 mL/min of flow, 80~100 MPa of processing pressure, temperature 30~40 DEG C, obtain rice bran protein nanoemulsions;
(2) instant tea powder extracts the preparation of composite lipidosome: 1%~1.5% instant tea powder is dissolved in 46.0%~48.3% pure water In, 5~6min is boiled, filtering removal impurity obtains instant tea powder extracting solution.By 1.5%~1.8% plant phosphatide and 0.2%~ 0.5% phytosterol is dissolved in ethyl alcohol (solid-liquid ratio 1:40~50), with Rotary Evaporators in 0.01~0.05 MPa, 40~42 DEG C, 50~60 min of rotary evaporation under 50~60 r/min, remove ethyl alcohol, make plant phosphatide and phytosterol in distilling flask Wall forms one layer of uniform film.Then 2%~2.4% maltitol and 2%~3.6% oligoisomaltose are dissolved into instant tea It in powder extracting solution, is then added in distilling flask, 50~60 min of film is washed at normal pressure, 40~42 DEG C, 50~60 r/min, Obtain liposome crude liquid.Liposome crude liquid is freezed into 1~2 h in ultra low temperature freezer (- 80~-60 DEG C), then in temperature 6 ~10 DEG C of defrostings, after melting completely, freeze at the same temperature again, freeze 2~3 times repeatedly, are restored to solution Under room temperature, obtains instant tea powder and extract composite lipidosome;
(3) preparation of rice bran protein tea beverage: by the rice bran protein nanoemulsions that step (1) obtains and the speed that step (2) obtains Molten tea powder extract liposome vibrates 60~90 min of mixing at 100~200 r/min, then they are passed through 0.22 μm of micropore Filter is filtered sterilizing, and sterile filling obtains rice bran protein tea beverage finished product.
Further, in step (1), the average grain diameter of the rice bran protein nanoemulsions is 144~159 nm.
Further, in step (2), the average grain diameter that the instant tea powder extracts composite lipidosome is 112~146 nm.
Further, in step (2), the polyphenol content that the instant tea powder extracts composite lipidosome is 87%~91%.
The present invention, which first passes through microjet high pressure homogenization technique, reduces the particle size of rice bran protein solution moderately, average grain Diameter is 144~159 nm, very close to the average grain diameter of lactalbumin lotion, to improve the dissolubility and solution body of rice bran protein It is stability.Then, instant tea powder extract is prepared by nanoscale liposome using multigelation method, average grain diameter is 112~146 nm, encapsulation rate (in terms of tea polyphenols) reach 87%~91%, effectively reduce polyphenol substance and rice bran egg in tealeaves The white probability directly contacted, avoids the formation of precipitating.After liposome embedded, main flavor substance is also not easy to be oxidized in tealeaves, The unique oral sensations of beverage can more permanently be kept.In addition, the maltitol that is added during liposome preparation and oligomeric different Maltose acts not only as beverage sweetener and liposome cryoprotector, and also good vegetable protein beverage is steady Determine agent.Above several technological incorporation are formd the preparation method of a set of rice bran protein tea beverage by the present invention.
The present invention has the beneficial effect that: (1) rice bran protein tea beverage of the present invention is to substitute milk system with rice bran protein lotion Make milk tea, can satisfy the demand of lactose intolerant patient;Rich in prebiotic agents, phosphatide and sterol, has and adjust enteron aisle Colony balance inhibits human body to the absorption of cholesterol and prevents the effect of cardiovascular disease;(2) rice bran protein prepared by the present invention Tea beverage finished product average grain diameter is 139~162 nm, can obviously inhibit the non-rancid precipitated and separated that vegetable protein beverage is common Phenomenon, product centrifugation rate is only 0.42%~0.64%;After room temperature stores 30d, beverage without layering, flocculation, deposited phenomenon, from Heart rate of deposition is 0.65%~0.79%, and stability is still suitable with the milk tea system of fresh milk production;It effectively reduces in tealeaves The probability that polyphenol substance is directly contacted with rice bran protein avoids the formation of precipitating, and rice bran protein is enable to substitute lactoprotein, soybean egg It is white, nutritional ingredient in rice bran was not only remained, but also do not influence the mouthfeel of tea beverage, and can be widely applied to liquid tea-drinking material products.
Specific embodiment
Below with reference to embodiment, the invention will be further described.
It is rice bran protein powder, instant tea powder used in the embodiment of the present invention, plant phosphatide, phytosterol, maltitol, low The raw-food materials such as polyisomaltose pass through routine business approach and obtain.
Embodiment 1
The present embodiment rice bran protein tea beverage is in terms of 100% by total weight, and composition of raw materials is as follows: rice bran protein powder 3%, instant green Tea powder 1%, soybean lecithin 1.5%, cupreol 0.2%, maltitol 2%, oligoisomaltose 2%, pure water 90.3%.
The preparation method of the present embodiment rice bran protein tea beverage, specifically includes the following steps:
(1) preparation of rice bran protein nanoemulsions: 3% rice bran protein powder is dissolved in 42% pure water, in 30 DEG C, 100 r/min Under be stirred to react 60 min, dissolve it sufficiently;Then high-pressure homogeneous processing is carried out to mixed solution using Microfluidizer, 80 mL/min of flow, 80 MPa of processing pressure, obtain rice bran protein nanoemulsions by 30 DEG C of temperature;
(2) instant tea powder extracts the preparation of composite lipidosome: 1% instant green tea powder being dissolved in 48.3% pure water, boils 5 Min, filtering removal impurity obtain instant tea powder extracting solution.1.5% soybean lecithin and 0.2% cupreol are dissolved in ethyl alcohol (material Liquor ratio 1:40), with Rotary Evaporators, 50 min of rotary evaporation, removal ethyl alcohol make phosphorus at 0.01 MPa, 40 DEG C, 50 r/min Rouge and sterol are in distilling flask inner wall one layer of uniform film of formation.Then 2% maltitol and 2% oligoisomaltose are dissolved It into instant tea powder extracting solution, is then added in distilling flask, 50 min of film is washed at normal pressure, 40 DEG C, 50 r/min, is obtained Liposome crude liquid.Liposome crude liquid is freezed into 1 h in ultra low temperature freezer (- 80 DEG C), is then thawed at 6 DEG C of temperature, to complete It after full thawing, freezes at the same temperature again, freezes 2 times repeatedly, be restored to solution under room temperature, obtain instant tea powder Extract composite lipidosome;
(3) preparation of rice bran protein tea beverage: by the rice bran protein nanoemulsions that step (1) obtains and the speed that step (2) obtains Molten tea powder extract liposome vibrates 60 min of mixing at 100 r/min, then they are carried out by 0.22 μm of millipore filter Filtration sterilization, sterile filling obtain rice bran protein tea beverage finished product.
Chaff protein nano lotion, instant tea powder extract composite lipidosome in the preparation method of the present embodiment rice bran protein tea beverage Measured with the particle diameter distribution of rice bran protein tea beverage using Nano ZS nano-particle size analysis instrument, average grain diameter be respectively 159 nm, 146 nm and 162 nm.
The encapsulation rate of instant tea powder extract liposome is (more with tea in the preparation method of the present embodiment rice bran protein tea beverage Phenol meter) it is measured using following methods: composite lipidosome and free tea polyphenols first are extracted with supercentrifugation separation instant tea powder, then are adopted Instant tea is measured with the spectrophotometry of " detection method of GB/T 8313-2018 Tea Polyphenols in Tea and catechin content " Total tea polyphenols mass concentration C in powder extracting solution0With free tea polyphenols mass concentration C1, it is calculated with following formula:.After measured, instant tea powder extract in the preparation method of the present embodiment rice bran protein tea beverage The encapsulation rate of liposome is 87%.
The centrifugation rate of rice bran protein beverage product and its room temperature storage 30d manufactured in the present embodiment uses following methods Measurement: accurately weighing 20 g samples, is centrifuged 15 min in 3000 r/min, weighs the weight of precipitate of centrifugation bottom of the tube, Each sample carries out 3 repetitions, is calculated by formula: centrifugation rate (%)=weight of precipitate (g)/Centrifuge A sample weight (g) × 100%.After measured, the centrifugation rate of rice bran protein tea beverage finished product manufactured in the present embodiment is 0.64%, is stored up in room temperature After hiding 30d, centrifugation rate is 0.79%.
Control group test: 3% rice bran protein powder is dissolved in 42% pure water, is stirred at 30 DEG C, 100 r/min anti- 60 min are answered, dissolve it sufficiently, obtain rice bran protein solution.1% instant green tea powder is dissolved in 48.3% pure water, is boiled 5 min, filtering removal impurity obtain instant tea powder extracting solution.By rice bran protein solution, instant tea powder extracting solution, 1.5% soybean phosphorus Rouge, 0.2% cupreol, 2% maltitol and 2% oligoisomaltose vibrated at 100 r/min mixing 60 min, then by it Sterilizing is filtered by 0.22 μm of millipore filter, sterile filling obtains control group beverage product.
It uses and measures the centrifugation rate of the control group beverage product with embodiment same procedure as 1.5%, room temperature storage After 30d, which there is apparent layering, precipitating, lipid precipitation phenomenon, and centrifugation rate is 2.2%, shows the present embodiment system Standby rice bran protein tea beverage is with good stability.
Embodiment 2
The present embodiment rice bran protein tea beverage is in terms of 100% by total weight, and composition of raw materials is as follows: rice bran protein powder 3.5%, instant Black tea powder 1.2%, corn phosphatidase 1 .6%, cupreol 0.3%, maltitol 2.2%, oligoisomaltose 3%, pure water 88.2%.
The preparation method of the present embodiment rice bran protein tea beverage, specifically includes the following steps:
(1) preparation of rice bran protein nanoemulsions: 3.5% rice bran protein powder is dissolved in 41% pure water, in 35 DEG C, 120 r/ It is stirred to react 75 min under min, dissolves it sufficiently.Then high-pressure homogeneous place is carried out to mixed solution using Microfluidizer Reason, 90 mL/min of flow, 90 MPa of processing pressure, obtain rice bran protein nanoemulsions by 35 DEG C of temperature.
(2) instant tea powder extracts the preparation of composite lipidosome: 1.2% instant black tea powder being dissolved in 47.2% pure water, is boiled 5.5 min are boiled, filtering removal impurity obtains instant tea powder extracting solution.1.6% corn phosphatide and 0.3% cupreol are dissolved in second In alcohol (solid-liquid ratio 1:45), with Rotary Evaporators at 0.03 MPa, 41 DEG C, 55 r/min 55 min of rotary evaporation, remove second Alcohol makes phosphatide and sterol form one layer of uniform film in distilling flask inner wall.Then 2.2% maltitol and 3% is oligomeric different Maltose is dissolved into instant tea powder extracting solution, is then added in distilling flask, washes film 55 at normal pressure, 41 DEG C, 55 r/min Min obtains liposome crude liquid.Liposome crude liquid is freezed into 1.5 h in ultra low temperature freezer (- 70 DEG C), then at 8 DEG C of temperature It thaws, after melting completely, freezes at the same temperature again, freeze 3 times repeatedly, be restored to solution under room temperature, obtain Composite lipidosome is extracted to instant tea powder.
(3) preparation of rice bran protein tea beverage: rice bran protein nanoemulsions and step (2) that step (1) obtains are obtained Instant tea powder extract composite lipidosome vibrate at 150 r/min mixing 80 min, then by they pass through 0.22 μm of millipore filter It is filtered sterilizing, sterile filling obtains rice bran protein tea beverage finished product.
Rice bran protein nanoemulsions, instant tea powder extract lipid in the preparation method of the present embodiment rice bran protein tea beverage The particle diameter distribution of body and rice bran protein tea beverage is measured using Nano ZS nano-particle size analysis instrument, and average grain diameter is respectively 147 Nm, 135 nm and 151 nm.
The encapsulation rate of instant tea powder extract liposome is (more with tea in the preparation method of the present embodiment rice bran protein tea beverage Phenol meter) it is measured using method same as Example 1.After measured, it is 90% that instant tea powder, which extracts the encapsulation rate of composite lipidosome,.
The centrifugation rate of rice bran protein beverage product and its room temperature storage 30d manufactured in the present embodiment uses and embodiment 1 identical method measurement, after measured, the centrifugation rate of rice bran protein beverage product manufactured in the present embodiment is 0.55%, room temperature After storing 30d, centrifugation rate is 0.72%.
Control group test: 3.5% rice bran protein powder is dissolved in 41% pure water, is stirred at 35 DEG C, 120 r/min 75 min are reacted, dissolve it sufficiently, obtain rice bran protein solution.1.2% instant black tea powder is dissolved in 47.2% pure water, 5.5 min are boiled, filtering removal impurity obtains instant tea powder extracting solution.By rice bran protein solution, instant tea powder extracting solution, 1.6% Corn phosphatide, 0.3% cupreol, 2.2% maltitol and 3% oligoisomaltose vibrate mixing 80 at 150 r/min Min, then they are filtered sterilizing by 0.22 μm of millipore filter, sterile filling obtains control group beverage product.
Control group beverage product centrifugation rate, after measured, control group beverage are measured using method same as Example 1 The centrifugation rate of finished product is 2.2%, and after room temperature stores 30d, which apparent layering, precipitating, lipid precipitation phenomenon occurs, Centrifugation rate is 2.8%, shows that rice bran protein beverage product manufactured in the present embodiment is with good stability.
Embodiment 3
The present embodiment rice bran protein tea beverage is in terms of 100% by total weight, and composition of raw materials is as follows: rice bran protein powder 4.2%, instant Dark green tea powder 1.5%, corn phosphatidase 1 .8%, stigmasterol 0.5%, maltitol 2.4%, oligoisomaltose 3.6%, pure water 86.0%.
The preparation method of the present embodiment rice bran protein tea beverage, specifically includes the following steps:
(1) preparation of rice bran protein nanoemulsions: 4.2% rice bran protein powder is dissolved in 40% pure water, in 40 DEG C, 200 r/ It is stirred to react 90 min under min, dissolves it sufficiently.Then high-pressure homogeneous place is carried out to mixed solution using Microfluidizer Reason, 100 mL/min of flow, 100 MPa of processing pressure, obtain rice bran protein nanoemulsions by 40 DEG C of temperature.
(2) instant tea powder extracts the preparation of composite lipidosome: 1.5% instant dark tea powder being dissolved in 46.0% pure water, is boiled 6min is boiled, filtering removal impurity obtains instant tea powder extracting solution.1.8% corn phosphatide and 0.5% stigmasterol are dissolved in ethyl alcohol (material Liquor ratio 1:50), with Rotary Evaporators, 60 min of rotary evaporation, removal ethyl alcohol make at 0.05 MPa, 42 DEG C, 60 r/min Phosphatide and sterol are in distilling flask inner wall one layer of uniform film of formation.Then by the oligomeric different malt of 2.4% maltitol and 3.6% Sugar is dissolved into instant tea powder extracting solution, is then added in distilling flask, washes film 60 at normal pressure, 42 DEG C, 60 r/min Min obtains liposome crude liquid.Liposome crude liquid is freezed into 2 h in ultra low temperature freezer (- 60 DEG C), then at 10 DEG C of temperature It thaws, after melting completely, freezes at the same temperature again, freeze 3 times repeatedly, be restored to solution under room temperature, obtain Composite lipidosome is extracted to instant tea powder.
(3) preparation of rice bran protein tea beverage: rice bran protein nanoemulsions and step (2) that step (1) obtains are obtained Instant tea powder extract composite lipidosome vibrate at 200 r/min mixing 90 min, then by they pass through 0.22 μm of millipore filter It is filtered sterilizing, sterile filling obtains rice bran protein tea beverage finished product.
Rice bran protein nanoemulsions, instant tea powder extract lipid in the preparation method of the present embodiment rice bran protein tea beverage The particle diameter distribution of body and rice bran protein tea beverage is measured using Nano ZS nano-particle size analysis instrument, and average grain diameter is respectively 144 Nm, 112 nm and 139nm.
The encapsulation rate of instant tea powder extract liposome is (more with tea in the preparation method of the present embodiment rice bran protein tea beverage Phenol meter) it is measured using method same as Example 1.After measured, the preparation method middling speed of the present embodiment rice bran protein tea beverage The encapsulation rate of molten tea powder extract liposome is 91%.
The centrifugation rate of rice bran protein beverage product and its room temperature storage 30d manufactured in the present embodiment uses and embodiment 1 identical method measurement, after measured, the centrifugation rate of rice bran protein beverage product manufactured in the present embodiment is 0.42%, room temperature After storing 30d, centrifugation rate is 0.65%.
Control group test: 4.2% rice bran protein powder is dissolved in 40% pure water, is stirred at 40 DEG C, 200 r/min anti- 90 min are answered, dissolve it sufficiently, obtain rice bran protein solution.1.5% instant dark tea powder is dissolved in 46% pure water, is boiled 5 min, filtering removal impurity obtain instant tea powder extracting solution.By rice bran protein solution, instant tea powder extracting solution, 1.8% corn phosphorus Rouge, 0.5% stigmasterol, 2.4% maltitol, 3.6% oligoisomaltose vibrated at 200 r/min mixing 90 min, then by it Sterilizing is filtered by 0.22 μm of millipore filter, sterile filling obtains control group beverage product.
Using the centrifugation rate of method measurement control group beverage product same as Example 1, after measured, control group drink The centrifugation rate for expecting finished product is 2.6%, and after room temperature stores 30d, the apparent layering of beverage appearance, precipitating, lipid are precipitated now As centrifugation rate is 3.4%, shows that rice bran protein beverage product manufactured in the present embodiment is with good stability.

Claims (9)

1. a kind of rice bran protein tea beverage, which is characterized in that be made of the raw material of following weight percent: 3%~4.2% rice bran egg White powder, 1%~1.5% instant tea powder, 1.5%~1.8% plant phosphatide, 0.2%~0.5% phytosterol, 2%~2.4% maltitol, 2%~3.6% oligoisomaltose, excess water.
2. rice bran protein tea beverage according to claim 1, which is characterized in that the lipidated protein of the rice bran protein powder >= 80%。
3. rice bran protein tea beverage according to claim 1 or claim 2, which is characterized in that the instant tea powder be instant black tea powder, Instant green tea powder or instant dark tea powder.
4. rice bran protein tea beverage described according to claim 1~one of 3, which is characterized in that the plant phosphatide is soybean phosphorus Rouge or corn phosphatide.
5. rice bran protein tea beverage described according to claim 1~one of 4, which is characterized in that the phytosterol is β-paddy steroid Alcohol or stigmasterol.
6. a kind of preparation method of the rice bran protein tea beverage as described according to claim 1~one of 5, which is characterized in that including Following steps:
(1) preparation of rice bran protein nanoemulsions: 3%~4.2% rice bran protein powder is dissolved in 40%~42% water, 30~40 DEG C, be stirred to react 60~90 min under 100~200 r/min, dissolve it sufficiently;Then using Microfluidizer to rice bran Albumen powder solution carries out high-pressure homogeneous processing, 80~100 mL/min of flow, 80~100 MPa of processing pressure, temperature 30~40 DEG C, obtain rice bran protein nanoemulsions;
(2) instant tea powder extracts the preparation of composite lipidosome: 1%~1.5% instant tea powder is dissolved in 46.0%~48.3% pure water In, 5~6min is boiled, filtering removal impurity obtains instant tea powder extracting solution;By 1.5%~1.8% plant phosphatide and 0.2%~ 0.5% phytosterol is dissolved in ethyl alcohol by the solid-liquid ratio of 1:40~50, with Rotary Evaporators in 0.01~0.05 MPa, 40~42 DEG C, 50~60 min of rotary evaporation under 50~60 r/min, remove ethyl alcohol, make plant phosphatide and phytosterol in distilling flask Wall forms one layer of uniform film;Then 2%~2.4% maltitol and 2%~3.6% oligoisomaltose are dissolved into instant tea It in powder extracting solution, is then added in distilling flask, 50~60 min of film is washed at normal pressure, 40~42 DEG C, 50~60 r/min, Obtain liposome crude liquid;Liposome crude liquid is freezed into 1~2 h in -80~-60 DEG C of ultra low temperature freezers, then temperature 6~ 10 DEG C of defrostings, after melting completely, freeze at the same temperature again, freeze 2~3 times repeatedly, are restored to solution often Under temperature, obtains instant tea powder and extract composite lipidosome;
(3) preparation of rice bran protein tea beverage: by the rice bran protein nanoemulsions that step (1) obtains and the speed that step (2) obtains Molten tea powder extract liposome vibrates 60~90 min of mixing at 100~200 r/min, then they are micro- by 0.22 μm Hole filter is filtered sterilizing, and sterile filling obtains rice bran protein tea beverage finished product.
7. a kind of preparation method of rice bran protein tea beverage according to claim 6, which is characterized in that described in step (1) The average grain diameter of rice bran protein nanoemulsions is 144~159 nm.
8. a kind of preparation method of rice bran protein tea beverage described according to claim 6 or 7, which is characterized in that in step (2), The average grain diameter that the instant tea powder extracts composite lipidosome is 112~146 nm.
9. a kind of preparation method of rice bran protein tea beverage according to one of claim 6~8, which is characterized in that step (2) In, the polyphenol content that the instant tea powder extracts composite lipidosome is 87%~91%.
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CN114601920A (en) * 2022-03-23 2022-06-10 宁波御坊堂生物科技有限公司 Oral liposome composition for improving bone joint health and preparation method thereof

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