CN109459419A - Fluorescence imaging system and optical path transmission assembly thereof - Google Patents
Fluorescence imaging system and optical path transmission assembly thereof Download PDFInfo
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- CN109459419A CN109459419A CN201710796813.XA CN201710796813A CN109459419A CN 109459419 A CN109459419 A CN 109459419A CN 201710796813 A CN201710796813 A CN 201710796813A CN 109459419 A CN109459419 A CN 109459419A
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- 230000003287 optical effect Effects 0.000 title claims description 17
- 238000000799 fluorescence microscopy Methods 0.000 title abstract description 5
- 230000005540 biological transmission Effects 0.000 title abstract 4
- 238000003384 imaging method Methods 0.000 claims abstract description 46
- 230000005284 excitation Effects 0.000 claims abstract description 17
- 239000011148 porous material Substances 0.000 claims description 6
- 239000013307 optical fiber Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 230000010287 polarization Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 230000000644 propagated effect Effects 0.000 description 3
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6478—Special lenses
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- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention relates to a fluorescence imaging system and a light path transmission component thereof. The fluorescence imaging system comprises an excitation light source for generating excitation light, a light path transmission component and an imaging device. The light path transmission assembly comprises a first motion platform, a second motion platform, a reflector, a mounting frame and an objective lens. The first motion stage is movable in a first direction. The second motion platform is arranged on the first motion platform and can move along a second direction relative to the first motion platform. The reflecting mirror comprises a first reflecting mirror and a second reflecting mirror, the first reflecting mirror is arranged on the first moving platform, and the second reflecting mirror is arranged on the second moving platform. The mounting bracket is movably arranged on the second motion platform. The objective lens is arranged on the mounting frame. The imaging device is arranged on the mounting frame and is positioned between the second reflector and the objective lens. The exciting light can irradiate on the sample through the objective lens after being reflected by the first reflecting mirror and the second reflecting mirror in sequence.
Description
Technical field
The present invention relates to optical technical fields, more particularly to a kind of fluoroscopic imaging systems and its optic path component.
Background technique
Traditional fluoroscopic imaging systems, mainly by two-dimensional movement sample, to realize that two-dimensional fluoroscopic is imaged, still, tradition
Fluoroscopic imaging systems can not the sample fixed to position carry out two-dimensional scanning.In order to solve foregoing problems, some fluorescence imagings
System has used optical fiber scanning technology or reflection mirror techniques, can carry out two-dimensional scanning to sample.
Optical fiber scanning technology is the other end of optical fiber then to be connected object lens and is fixed on two dimension laser coupled entering light fibre
On mobile platform, laser two-dimensional scanning on sample is realized by the position of moving fiber.Reflection mirror techniques are using reflection
The space optical path that galvanometer is built, the inclination angle by controlling galvanometer allow light to be incident on object lens at different angles, finally focus on
The different location of sample realizes the two-dimensional scanning to sample.
It, can heavy losses light intensity when laser transmits in a fiber but for optical fiber scanning technology.In addition, optical fiber is to sharp
The degree of polarization of light has a significant impact, and cannot apply in the fluorescence detection that polarization is differentiated.For reflecting mirror techniques, model is scanned
It encloses the limitation by objective lens entrance mouth size, when incidence angle is bigger than normal, hot spot can be deformed, and seriously affect fluorescence efficiency, therefore
Reflection mirror techniques can only work in micro- a small range.
Summary of the invention
Based on this, it is necessary in view of the above-mentioned problems, two-dimensional scanning can be carried out to the fixed sample in position by providing one kind
Fluoroscopic imaging systems and its optic path component.
A kind of optic path component, comprising:
First motion platform can be moved along first direction；
Second motion platform is set on first motion platform, relatively described first fortune of second motion platform
Moving platform can move in a second direction；
Reflecting mirror, including the first reflecting mirror and the second reflecting mirror, it is flat that first reflecting mirror is set to first movement
On platform, second reflecting mirror is set on second motion platform；
Mounting rack is movably arranged on the second motion platform；And
Object lens are set on mounting rack, and it is opposite that the object lens with second reflecting mirror are located at the mounting rack
Two sides；
Wherein, the exciting light that excitation light source generates successively is reflected by first reflecting mirror and second reflecting mirror
Afterwards, it can be radiated on sample by the object lens, mobile first motion platform and second motion platform are described to swash
Shining can move synchronously with the object lens.
The mounting rack is set on second motion platform by moving assembly in one of the embodiments, institute
Stating moving assembly includes fixed plate and three-dimensional mobile platform, and the fixed plate is set on second motion platform, and described three
Dimension mobile platform is set in the fixed plate, and the mounting rack is set in the three-dimensional mobile platform, described three-dimensional mobile
The movement of platform is able to drive the mounting rack movement.
Second reflecting mirror is set in the fixed plate in one of the embodiments, and the fixed plate offers
Intercommunicating pore is incident on second reflecting mirror by the intercommunicating pore after the exciting light is reflected by first reflecting mirror
On.
Second reflecting mirror includes the second support column and the second reflecting optics in one of the embodiments, and described the
Two support columns are set in the fixed plate, and second reflecting optics are adjustably set on second support column.
The mounting rack includes bottom plate, side plate and top plate in one of the embodiments, and the bottom plate is set to described three
Tie up on mobile platform, the quantity of the side plate be it is multiple, the both ends of multiple side plates respectively with the bottom plate and the top plate
It is connected, the two neighboring side plate is connected, and the side plate offers the through-hole passed through for the exciting light, and the object lens are set
It sets on the side plate.
First reflecting mirror includes the first support column and the first reflecting optics in one of the embodiments, and described the
One support column is set on first motion platform, and first reflecting optics are adjustably set to first support column
On.
The exciting light is after first reflecting mirror reflection in one of the embodiments, along the second direction
It is incident on second reflecting mirror, the exciting light passes through described after second reflecting mirror reflection along third direction
Object lens, and it is vertical two-by-two between the first direction, the second direction and the third direction three.
A kind of fluoroscopic imaging systems, comprising:
Excitation light source, for generating exciting light；
Optic path component as described in above-mentioned any one；And
Imaging device is set on mounting rack, and the imaging device is between the second reflecting mirror and object lens, sample reflection
Optical signal can be received by object lens by the imaging device, to form image.
The imaging device includes imaging sensor and the first half-reflecting half mirror, the figure in one of the embodiments,
As sensor is set on the mounting rack, first half-reflecting half mirror is set in the mounting rack, second reflection
The exciting light that mirror reflects can be irradiated on the sample by first half-reflecting half mirror and the object lens, described
After the optical signal of sample reflection passes through the object lens, described image biography can be reflected by first half-reflecting half mirror
On sensor, to be received by described image sensor.
The imaging device further includes lighting source and the second half-reflecting half mirror, the photograph in one of the embodiments,
Mingguang City source is set on the mounting rack, and the lighting source is between described image sensor and the object lens, and described
Two half-reflecting half mirrors are set in the mounting rack, second half-reflecting half mirror be located at first half-reflecting half mirror with it is described
Between object lens, the irradiation light that the lighting source generates passes through the object lens after capable of reflecting by second half-reflecting half mirror
It is irradiated on the sample, the exciting light and the optical signal can pass through second half-reflecting half mirror.
Above-mentioned fluoroscopic imaging systems and its optic path component have at least the following advantages:
The exciting light that excitation light source generates successively after the first reflecting mirror and the reflection of the second reflecting mirror, then passes through object lens
It is radiated on sample, the optical signal of sample reflection can be received by object lens by imaging device, to form image.Pass through mobile the
One motion platform and the second motion platform, exciting light are moved synchronously with object lens, so as to carry out two-dimensional scanning to sample.Fluorescence
Imaging system can carry out two-dimensional scanning to the sample that position is completely fixed, and scanning area is larger, has a wide range of application.The light of laser
The problem of road is space optical path, avoids the loss of optical fiber bring light intensity, polarization loss.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of fluoroscopic imaging device in an embodiment；
Fig. 2 is the partial schematic diagram of fluoroscopic imaging device shown in Fig. 1；
Fig. 3 is the schematic diagram that exciting light transmits in optic path component.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, with reference to the accompanying drawing to the present invention
Specific embodiment be described in detail.Many details are explained in the following description in order to fully understand this hair
It is bright.But the invention can be embodied in many other ways as described herein, those skilled in the art can be not
Similar improvement is done in the case where violating intension of the present invention, therefore the present invention is not limited to the specific embodiments disclosed below.
It should be noted that it can directly on the other element when element is referred to as " being fixed on " another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it, which can be, is directly connected to
To another element or it may be simultaneously present centering elements.Term as used herein " vertical ", " horizontal ", " left side ",
" right side " and similar statement for illustrative purposes only, are not meant to be the only embodiment.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.
Fig. 1 and Fig. 3 is please referred to, the fluoroscopic imaging systems in an embodiment can carry out two to the fixed sample in position
Dimension scanning, scanning range is larger, has a wide range of application.Specific in present embodiment, which includes excitation light source
(not shown), optic path component 10 and imaging device 20.
Excitation light source can be radiated at for generating exciting light 30 after exciting light 30 is transmitted by optic path component 10
On sample.Specifically, excitation light source is laser, and exciting light 30 is laser.It is understood that in other embodiments,
Excitation light source may be light emitting diode.
Optic path component 10 include the first motion platform 100, the second motion platform 200, moving assembly 300, reflecting mirror,
Mounting rack 500 and object lens 600.First motion platform 100 can move along a first direction.Second motion platform 200 is set to
On first motion platform 100, the second motion platform 200 can move in a second direction relative to the first motion platform 100.Moving assembly
300 include fixed plate 310 and three-dimensional mobile platform 320.Fixed plate 310 is set on the second motion platform 200, fixed plate 310
Offer the intercommunicating pore 312 passed through for exciting light 30.
Three-dimensional mobile platform 320 is set in fixed plate 310, and three-dimensional mobile platform 320 can be along three different sides
To movement.In one embodiment, three-dimensional mobile platform 320 can be moved along first direction, second direction and third direction.And
And it is vertical two-by-two between first direction, second direction and third direction three.Specific in present embodiment, first direction Y
Axis direction, second direction are Z-direction, and third direction is X-direction.
Reflecting mirror includes the first reflecting mirror 410 and the second reflecting mirror 420, and it is flat that the first reflecting mirror 410 is set to the first movement
On platform 100, the exciting light 30 that excitation light source generates can be incident on the first reflecting mirror 410.Exciting light 30 is by the first reflection
After mirror 410 reflects, it can project in a second direction.Specifically, the first reflecting mirror 410 includes that the first support column 412 and first is anti-
Mirror surface 414 is penetrated, the first support column 412 is set on the first motion platform 100, and the first mirror surface 414 is adjustably set to
On first support column 412.By adjusting the position of the first mirror surface 414, it is ensured that exciting light 30 after reflection edge always
Second direction projects.
Second reflecting mirror 420 is set on the second motion platform 200.In one embodiment, the second reflecting mirror 420 passes through solid
Fixed board 310 is set on the second motion platform 200.After the exciting light 30 that excitation light source generates is reflected by the first reflecting mirror 410,
It can be incident on by intercommunicating pore 312 on second reflecting mirror 420.Exciting light 30 is after the reflection of the second reflecting mirror 420, Neng Gouyan
Third direction projects.Specifically, the second reflecting mirror 420 includes the second support column 422 and the second mirror surface 424, the second support
Column 422 is set in fixed plate 310, and the second mirror surface 424 is adjustably set on the second support column 422.By adjusting
The position of second mirror surface 424, it is ensured that the exciting light 30 after reflection is projected along third direction always.
Referring to Figure 2 together, mounting rack 500 is movably arranged on the second motion platform 200.In one embodiment,
Mounting rack 500 is set on the second motion platform 200 by moving assembly 300.Mounting rack 500 is set to three-dimensional mobile platform
On 320, the movement of three-dimensional mobile platform 320 is able to drive the movement of mounting rack 500.
Specifically, mounting rack 500 includes bottom plate 510, side plate 520 and top plate 530.Bottom plate 510 is set to three-dimensional mobile flat
On platform 320.The quantity of side plate 520 be it is multiple, the both ends of the side plate 520 are connected with bottom plate 510 and top plate 530 respectively, phase
Adjacent two side plates 520 are connected, and side plate 520 offers the through-hole 522 passed through for exciting light 30.Specifically, the quantity of side plate 520
It is three, and two of them side plate 520 is arranged along third direction interval, wherein one side is not closed out mounting rack 500, with convenient
The components of imaging device 20 are installed.It offers along spaced two side plates 520 of third direction and passes through for exciting light 30
Through-hole 522, two through-holes 522 are coaxially disposed.Top plate 530 offers the first pilot hole 532 and the second pilot hole 534, and first
Pilot hole 532 and the second pilot hole 534 are arranged along third direction interval.
Object lens 600 are set on mounting rack 500, and object lens 600 and the second reflecting mirror 420 are located at mounting rack 500 relatively
Two sides.Excitation light source generate exciting light 30 successively by the first reflecting mirror 410 and the second reflecting mirror 420 reflection after, can
It is radiated on sample by object lens 600.In present embodiment, object lens 600 are set on side plate 520, object lens 600 and through-hole 522
Coaxial arrangement, and the extending direction of object lens 600 extends along third direction, so that completely logical along the exciting light 30 that third direction is propagated
Cross 600 center of object lens.
Imaging device 20 is set on mounting rack 500, imaging device 20 between the second reflecting mirror 420 and object lens 600,
The optical signal of sample reflection can be received by object lens 600 by imaging device 20, to form image.In one embodiment, imaging
Device 20 includes imaging sensor 710, lighting source 720, the first half-reflecting half mirror 730 and the second half-reflecting half mirror 740.Image
Sensor 710 and lighting source 720 are arranged at intervals on the top plate 530 of mounting rack 500, and lighting source 720 is located at image biography
Between sensor 710 and object lens 600.First half-reflecting half mirror 730 and the second half-reflecting half mirror 740 are arranged at intervals on bottom plate 510,
Second half-reflecting half mirror 740 is between the first half-reflecting half mirror 730 and object lens 600.Excitation after the reflection of second reflecting mirror 420
Light 30 can pass sequentially through the first half-reflecting half mirror 730, the second half-reflecting half mirror 740 and object lens 600 and be irradiated on sample.
The position of first half-reflecting half mirror 730 is corresponding with the position of imaging sensor 710, the first half-reflecting half mirror 730
It is connected between imaging sensor 710 by the first pilot hole 532, so that after the optical signal of sample reflection passes through object lens 600,
It can be reflected on imaging sensor 710 by the first half-reflecting half mirror 730, to be received by imaging sensor 710, image sensing
Device 710 is capable of forming image after receiving optical signal.The position of second half-reflecting half mirror 740 is opposite with the position of lighting source 720
It answers, and is connected between the second half-reflecting half mirror 740 and lighting source 720 by the second pilot hole 534, so that lighting source
After 720 irradiation lights issued are reflected by the second half-reflecting half mirror 740, it can be radiated on sample by object lens 600, to sample
Product are illuminated.
Specifically, the first half-reflecting half mirror 730 and the second half-reflecting half mirror 740 are half-reflection and half-transmission prism, and the first half anti-
Pellicle mirror 730 coincides with projection of the imaging sensor 710 on bottom plate 510, the second half-reflecting half mirror 740 and lighting source
720 projection on bottom plate 510 coincides.In one embodiment, imaging sensor 710 is CCD (Charge Coupled
Device, charge coupled cell) imaging sensor, lighting source 720 is LED (Light-Emitting Diode, light-emitting diodes
Pipe) light source.
The working principle of above-mentioned fluoroscopic imaging systems specifically:
Excitation light source generates exciting light 30, which is injected into along the y axis on the first reflecting mirror 410, by the
After the reflection of one reflecting mirror 410, it is injected on the second reflecting mirror 420 along Z-direction.Exciting light 30 is anti-by the second reflecting mirror 420
It after penetrating, is emitted along the x axis, after finally passing sequentially through the first half-reflecting half mirror 730, the second half-reflecting half mirror 740 and object lens 600
It is irradiated on sample.Also, by adjusting three-dimensional mobile platform 320, move object lens 600 along the y axis, or along Z axis side
To movement, the exciting light 30 propagated along the x axis is made to pass through the center of object lens 600 completely.The irradiation light that lighting source 720 generates
After the reflection of the second half-reflecting half mirror 740, it is irradiated on sample by object lens 600.
After exciting light 30 is irradiated on sample, sample is capable of reflecting light signal.The optical signal of sample reflection passes sequentially through object
After mirror 600 and the second half-reflecting half mirror 740, it can be reflected on imaging sensor 710, scheme by the first half-reflecting half mirror 730
Image is formed after receiving optical signal as sensor 710.By adjusting three-dimensional mobile platform 320, move object lens 600 along the x axis
It is dynamic, so as to adjust the focal length of object lens 600, to guarantee blur-free imaging.
When carrying out two-dimensional scanning to sample, moves the first motion platform 100 along the y axis, make the second motion platform
200 move along Z-direction, and exciting light 30 is propagated along Y direction, Z-direction and X-direction always.First motion platform 100
And second motion platform 200 movement, be able to drive object lens 600 movement, along Z-direction outgoing exciting light 30 and object lens 600
It moves synchronously, so as to carry out two-dimensional scanning to sample, and then obtains the image of entire sample.
Above-mentioned fluoroscopic imaging systems can carry out two-dimensional scanning to the sample that position is completely fixed, and scanning area is big, application
Range is wide.The problem of optical path of exciting light 30 is space optical path, avoids optical fiber bring light intensity loss, polarization loss.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of optic path component characterized by comprising
First motion platform can be moved along first direction；
Second motion platform is set on first motion platform, and relatively described first movement of second motion platform is flat
Platform can move in a second direction；
Reflecting mirror, including the first reflecting mirror and the second reflecting mirror, first reflecting mirror are set on first motion platform,
Second reflecting mirror is set on second motion platform；
Mounting rack is movably arranged on the second motion platform；And
Object lens are set on mounting rack, the object lens and second reflecting mirror two sides that be located at the mounting rack opposite；
Wherein, excitation light source generate exciting light successively by first reflecting mirror and second reflecting mirror reflection after, energy
It is enough radiated on sample by the object lens, mobile first motion platform and second motion platform, the exciting light
It can be moved synchronously with the object lens.
2. optic path component according to claim 1, which is characterized in that the mounting rack is set to by moving assembly
On second motion platform, the moving assembly includes fixed plate and three-dimensional mobile platform, and the fixed plate is set to described
On second motion platform, the three-dimensional mobile platform is set in the fixed plate, and the mounting rack is set to the three-dimensional shifting
On moving platform, the movement of the three-dimensional mobile platform is able to drive the mounting rack movement.
3. optic path component according to claim 2, which is characterized in that second reflecting mirror is set to the fixation
On plate, the fixed plate offers intercommunicating pore and passes through the intercommunicating pore after the exciting light is reflected by first reflecting mirror
It is incident on second reflecting mirror.
4. optic path component according to claim 3, which is characterized in that second reflecting mirror includes the second support column
And second reflecting optics, second support column are set in the fixed plate, second reflecting optics are adjustably arranged
In on second support column.
5. optic path component according to claim 2, which is characterized in that the mounting rack includes bottom plate, side plate and top
Plate, the bottom plate are set in the three-dimensional mobile platform, and the quantity of the side plate is multiple, the both ends point of multiple side plates
It is not connected with the bottom plate and the top plate, the two neighboring side plate is connected, and the side plate is offered for the excitation
The through-hole that light passes through, the object lens setting is on the side plate.
6. optic path component according to claim 1, which is characterized in that first reflecting mirror includes the first support column
And first reflecting optics, first support column are set on first motion platform, first reflecting optics are adjustable
Ground is set on first support column.
7. optic path component according to claim 1, which is characterized in that the exciting light passes through first reflecting mirror
It after reflection, is incident on second reflecting mirror along the second direction, the exciting light is reflected by second reflecting mirror
Afterwards, along third direction by the object lens, and two between the first direction, the second direction and the third direction three
Two is vertical.
8. a kind of fluoroscopic imaging systems characterized by comprising
Excitation light source, for generating exciting light；
Optic path component as described in claim 1~7 any one；And
Imaging device is set on mounting rack, and the imaging device is between the second reflecting mirror and object lens, the light of sample reflection
Signal can be received by object lens by the imaging device, to form image.
9. fluoroscopic imaging systems according to claim 8, which is characterized in that the imaging device include imaging sensor and
First half-reflecting half mirror, described image sensor are set on the mounting rack, and first half-reflecting half mirror is set to described
In mounting rack, the exciting light that second reflecting mirror reflects can pass through first half-reflecting half mirror and the object lens
It is irradiated on the sample, it, can be anti-by described the first half after the optical signal of sample reflection passes through the object lens
Pellicle mirror is reflected into described image sensor, to be received by described image sensor.
10. fluoroscopic imaging systems according to claim 9, which is characterized in that the imaging device further includes lighting source
And second half-reflecting half mirror, the lighting source are set on the mounting rack, the lighting source is located at described image sensing
Between device and the object lens, second half-reflecting half mirror is set in the mounting rack, and second half-reflecting half mirror is located at
Between first half-reflecting half mirror and the object lens, irradiation light that the lighting source generates can be anti-by described the second half
It is irradiated on the sample after pellicle mirror reflection by the object lens, the exciting light and the optical signal can be by described
Second half-reflecting half mirror.
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| CN201710796813.XA CN109459419B (en) | 2017-09-06 | 2017-09-06 | Fluorescent imaging system and optical path transmission assembly thereof |
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| CN201710796813.XA CN109459419B (en) | 2017-09-06 | 2017-09-06 | Fluorescent imaging system and optical path transmission assembly thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112305741A (en) * | 2020-05-27 | 2021-02-02 | 南方科技大学 | Multi-focus optical system |
| CN114112791A (en) * | 2021-11-16 | 2022-03-01 | 广州市元奥仪器有限公司 | Portable double-optical-path schlieren instrument |
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| CN112305741A (en) * | 2020-05-27 | 2021-02-02 | 南方科技大学 | Multi-focus optical system |
| CN112305741B (en) * | 2020-05-27 | 2022-07-15 | 南方科技大学 | Axial multifocal optical system |
| CN114112791A (en) * | 2021-11-16 | 2022-03-01 | 广州市元奥仪器有限公司 | Portable double-optical-path schlieren instrument |
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| CN109459419B (en) | 2024-08-06 |
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