CN109453421A - For promoting the preparation method of the nano fiber scaffold of wound healing - Google Patents
For promoting the preparation method of the nano fiber scaffold of wound healing Download PDFInfo
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- CN109453421A CN109453421A CN201811376333.9A CN201811376333A CN109453421A CN 109453421 A CN109453421 A CN 109453421A CN 201811376333 A CN201811376333 A CN 201811376333A CN 109453421 A CN109453421 A CN 109453421A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0033—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0004—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0014—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0085—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/009—Materials resorbable by the body
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0092—Electro-spinning characterised by the electro-spinning apparatus characterised by the electrical field, e.g. combined with a magnetic fields, using biased or alternating fields
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4382—Stretched reticular film fibres; Composite fibres; Mixed fibres; Ultrafine fibres; Fibres for artificial leather
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
Abstract
The present invention provides a kind of preparation method of nano fiber scaffold for promoting wound healing, includes the following steps: that (1) prepares GO using Hummers method, and dry in an oven after being dialysed with distilled water, carries out dialysis purification to collagen；(2) by the collagen after dialysis, GO is added in PVA solution with treated, obtains PVA/ collagen/GO spinning solution；(3) above-mentioned spinning solution is placed in electrospinning device, the spinning parameter of spinning solution is set, start the electrospinning device, it is received on receiving device under spinning solution electrostatic interaction and generates jet stream, nano fiber scaffold is obtained, by the nano fiber scaffold vacuum freeze drying and sterilization treatment.Preparation method of the invention, which mainly passes through, improves bioactivity using the advantages of natural and synthetic polymer and using GO nano particle, realizes the specific characteristic of the nano fiber scaffold of preparation.
Description
Technical field
The invention belongs to the preparation technical fields of nano fiber scaffold, and in particular to a kind of for promoting receiving for wound healing
The preparation method of rice fibrous framework.
Background technique
In recent years, scientists, which are being studied, treats wound damage, the method for accelerating wound healing.In all enhancing wounds
In healing aspect innovative approach, the nano fiber scaffold (abbreviation electro spinning nano fiber bracket) of electrostatic spinning technique preparation is had become
One Important Platform.Since electro spinning nano fiber bracket can simulate natural extra-cellular matrix structure, (ECMs- is by the egg that interweaves
White matter fiber composition), and cell adherence, activity, differentiation and proliferation can be enhanced.Although these are as extra-cellular matrix structure
Multiple technologies manufacture can be used in the nano-bracket of substitute, such as mutually separation, melt-blown and self assembly etc..But electrostatic spinning technique
The nano fiber scaffold of manufacture is considered as the most simple and method with benefit, because it has high surface area and porosity,
And better oxygen permeability, while wound location hydrops can be prevented.
Various natural polymers (such as fibrin, elastin laminin, silk) and synthetic polymer (such as polyvinyl alcohol, poly- cream
Acid, PLGA etc.) it all can be used for Electrospinning manufacture nano fiber scaffold, for imitating tissue (such as ECMs).
Natural polymer usually has good biocompatibility and biodegradable, and has good absorbent properties, and synthesizes poly-
Closing object has good mechanical performance and thermal stability.Therefore, based on natural and synthetic polymer blends multifunctional polymeric
Object is possible to the bracket for becoming to have excellent properties, can promote wound healing.
PVA is a kind of synthetic polymer with suitable biomedical applications characteristic, has been widely used as wound dressing
Timbering material.However, collagen (natural polymer) can be used as the natural substrate of cell attachment and proliferation.In order to further mention
The bioactivity of high mixed polymer, is used for active nanoparticles, such as with the oxidation of adsorption of protein matter
Graphene (GO).GO has proved to be the suitable material of biological applications, because it has surprising biological characteristics, biofacies
Capacitive and its effect in accelerating wound healing.Based on background above, the present invention is by tri- kinds of PVA, collagen and GO materials
Characteristic combines, and using electrostatic spinning technique, designs a kind of preparation method of nano fiber scaffold for promoting wound healing.
Summary of the invention
The present invention provides a kind of preparation method of nano fiber scaffold for promoting wound healing, prepared by the present invention to receive
Rice fibrous framework has biocompatibility preferably and the stable characteristic of framework, can effectively accelerating wound healing.
Step S1: graphene oxide (GO) is prepared using Hummers method；
Step S2: the graphene oxide being dialysed with distilled water, obtains GO sample, and the GO sample is carried out
It collects, is placed in and dries in an oven, obtain GO finished product；
Step S3: the GO finished product adds deionized water to be ultrasonically treated, and obtains GO solution；
Step S4: dialysis purification is carried out to collagen, obtains collagen sterling；
Step S5: simultaneously stirring and dissolving is added in deionized water in polyvinyl alcohol (PVA), obtains PVA solution；
Step S6: the collagen sterling and the GO solution are added into the PVA solution, are stirred
It is even, obtain polyvinyl alcohol/collagen/graphene oxide spinning solution；
Step S7: polyvinyl alcohol/collagen/graphene oxide spinning solution that step S6 is obtained is placed in electrostatic spinning and sets
In standby syringe, apply high-voltage electrostatic field between the spinning head of syringe and the reception device of ground connection, the poly- second is set
Enol/collagen/graphene oxide spinning solution spinning parameter；Polyvinyl alcohol/the collagen/graphene oxide is spun
Silk liquid generates jet stream under electrostatic interaction, obtains nano fiber scaffold in the reception device；
Step S8: the nano fiber scaffold is subjected to vacuum freeze drying and sterilization treatment.
In some embodiments, in step S2, the dialysis time is 24-48 hours.
In some embodiments, in step S2, the drying condition is 60-65 DEG C, and the drying time is that 12-48 is small
When.
In some embodiments, in step s3, the time of the ultrasonic treatment is 2-10 hours.
In some embodiments, in step S4, the collagen is the collagen of medical grade, and is Type I collagen
Albumen, typeⅡ Collagen or III collagen type, the dialysis time are 24-48 hours.
In some embodiments, in step S5, the mass percentage concentration of the PVA solution is 5%-15%, described to stir
Mixing temperature condition is 80 DEG C, and the mixing time is 2-5 hours.
In some embodiments, the GO in the GO solution is spun in the polyvinyl alcohol/collagen/graphene oxide
Mass percent in silk liquid is 0.1%-0.5%, and the collagen is in the polyvinyl alcohol/collagen/graphite oxide
Mass percent in alkene spinning solution is 2%-10%, and the temperature condition of the stirring is room temperature, and the time of the stirring is 12-
24 hours.
In some embodiments, in step S7, the spinning parameter is provided that spinning voltage is 15-30KV, connects
Receiving distance is 10-20cm, and propulsion rate is 0.1-1mL/min, and the receiving device is roller, and the revolving speed that receives of the roller is
100-300r/min。
In some embodiments, the initial temperature of vacuum freeze drying is set as -50 DEG C to -20 DEG C in step S6, vacuum
The time of freeze-drying is 24-48 hours, and sterilization method is ethylene oxide (EO) sterilizing.
In some embodiments, the present invention uses the preparation method of electrostatic spinning, by natural polymer collagen, closes
It is combined at polymer P VA and GO performance, obtained nano fiber scaffold can simulate natural extracellular matrix structure.PVA tool
There are good mechanical properties, stable physical property；Collagen can be used as the substrate of cell attachment and proliferation, and it is viscous can to enhance cell
Attached, movable, differentiation and proliferation；GO further increases the bioactivity of mixed polymer, improves adsorption of protein matter.Compared to
The prior art, the nano fiber scaffold that invention is had the advantage that 1, prepared using electrostatic spinning technique, by PVA and collagen egg
The white structure as fiber effectively simulates natural extracellular matrix structure；2, nano fiber scaffold prepared by the present invention has good
Good mechanical performance, stable physical property and good biocompatibility；3, nano fiber scaffold prepared by the present invention, glue
The addition of former albumen can be used as the substrate of cell attachment and proliferation, enhancing cell adherence, activity, differentiation and proliferation；4, of the invention
The nano fiber scaffold of preparation, GO further increase the bioactivity of mixed polymer, to improve adsorption of protein matter.
Detailed description of the invention
Fig. 1 is the flow chart of the preparation method of the nano fiber scaffold for promoting wound healing of invention.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, and reference
Attached drawing, the present invention is described in more detail.
Embodiment one
The present invention provides a kind of preparation method of nano fiber scaffold for promoting wound healing, the specific steps are as follows:
1, the synthesis of graphene oxide (GO)
Step S1: graphene oxide (GO) is prepared using Hummers method；
Step S2: GO in step S1 is dialysed (dialysis time is 24 hours) with distilled water, by what is obtained after dialysis
GO sample is collected, and is placed in dry (drying temperature is 60 DEG C, and drying time is 12 hours) in an oven, is obtained GO finished product；
Step S3: the GO finished product after dry in step S2 adds deionized water to be ultrasonically treated (ultrasonic time is 2 hours),
Obtain GO solution；
2, the purifying of collagen
Step S4: dialysis purification (dialysis time is 24 hours) is carried out to medical grade Type I collagen albumen, obtains collagen
Sterling；
3, prepared by nano fiber scaffold
Step S5:PVA is added in deionized water and stirs 2 hours at 80 DEG C, and dissolution obtains PVA solution, PVA solution
Mass percentage concentration is 5%；
Step S6: the medical grade Type I collagen albumen after dialysing described in step S4 and GO solution are added to step S5 system
In standby PVA solution, the mass percentage concentration that the mass percentage concentration of final collagen is 0.1%, GO is 5%, at room temperature
Stirring 12 hours is uniformly mixed solution, obtains polyvinyl alcohol/collagen/graphene oxide spinning solution；
Step S7: polyvinyl alcohol/collagen/graphene oxide spinning solution that step S6 is obtained is placed in electrostatic spinning and sets
In standby syringe, apply high-voltage electrostatic field between the spinning head of syringe and the reception device of ground connection, the poly- second is set
(spinning voltage 20KV, receive distance is 15cm to enol/collagen/graphene oxide spinning solution spinning parameter, is promoted
Rate is 0.2mL/min, and it is 200r/min that roller, which receives revolving speed)；Polyvinyl alcohol/collagen/graphene oxide the spinning
Liquid generates jet stream under electrostatic interaction, receives to obtain nano fiber scaffold on roller；
Step S8: the nano fiber scaffold is subjected to low-temperature vacuum drying (initial temperature is set as -30 DEG C) 36 hours
With use EO mode to carry out sterilization treatment.
Embodiment two
1, the synthesis of graphene oxide (GO)
Step S1: graphene oxide (GO) is prepared using Hummers method；
Step S2: GO in step S1 is dialysed (dialysis time is 36 hours) with distilled water, by what is obtained after dialysis
GO sample is collected, and is placed in dry (drying temperature is 62 DEG C, and drying time is 24 hours) in an oven, is obtained GO finished product；
Step S3: the GO finished product after dry in step S2 adds deionized water to be ultrasonically treated (ultrasonic time is 5 hours),
Obtain GO solution；
2, the purifying of collagen
Step S4: dialysis purification (dialysis time is 36 hours) is carried out to medical level type II collagen albumen, obtains collagen egg
Bai Chunpin；
3, prepared by nano fiber scaffold
Step S5:PVA is added in deionized water and stirs 3 hours at 80 DEG C, and dissolution obtains PVA solution, PVA solution
Mass percentage concentration is 10%；
Step S6: the medical level type II collagen albumen after dialysing described in step S4 and GO solution are added to step S5 system
In standby PVA solution, the mass percentage concentration that the mass percentage concentration of final collagen is 0.3%, GO is 2%, at room temperature
Stirring 18 hours is uniformly mixed solution, obtains polyvinyl alcohol/collagen/graphene oxide spinning solution；
Step S7: polyvinyl alcohol/collagen/graphene oxide spinning solution that step S6 is obtained is placed in electrostatic spinning and sets
In standby syringe, apply high-voltage electrostatic field between the spinning head of syringe and the reception device of ground connection, the poly- second is set
(spinning voltage 15KV, receive distance is 10cm to enol/collagen/graphene oxide spinning solution spinning parameter, is promoted
Rate is 0.1mL/min, and it is 100r/min that roller, which receives revolving speed)；Polyvinyl alcohol/collagen/graphene oxide the spinning
Liquid generates jet stream under electrostatic interaction, receives to obtain nano fiber scaffold on roller；
Step S8: the nano fiber scaffold is subjected to low-temperature vacuum drying (initial temperature is set as -20 DEG C) 24 hours
With use EO mode to carry out sterilization treatment.
Embodiment three
1, the synthesis of graphene oxide (GO)
Step S1: graphene oxide (GO) is prepared using Hummers method；
Step S2: GO in step S1 is dialysed (dialysis time is 48 hours) with distilled water, by what is obtained after dialysis
GO sample is collected, and is placed in dry (drying temperature is 65 DEG C, and drying time is 48 hours) in an oven, is obtained GO finished product；
Step S3: the GO finished product after dry in step S2 add deionized water to be ultrasonically treated (ultrasonic time is 10 small
When), obtain GO solution；
2, the purifying of collagen
Step S4: dialysis purification (dialysis time is 48 hours) is carried out to medical III type collagen albumen, obtains collagen egg
Bai Chunpin；
3, prepared by nano fiber scaffold
Step S5:PVA is added in deionized water and stirs 5 hours at 80 DEG C, and dissolution obtains PVA solution, PVA solution
Mass percentage concentration is 15%；
Step S6: the medical III type collagen albumen after dialysing described in step S4 and GO solution are added to step S5 system
In standby PVA solution, the mass percentage concentration that the mass percentage concentration of final collagen is 0.5%, GO is 10%, at room temperature
Stirring 24 hours is uniformly mixed solution, obtains polyvinyl alcohol/collagen/graphene oxide spinning solution；
Step S7: polyvinyl alcohol/collagen/graphene oxide spinning solution that step S6 is obtained is placed in electrostatic spinning and sets
In standby syringe, apply high-voltage electrostatic field between the spinning head of syringe and the reception device of ground connection, the poly- second is set
(spinning voltage 30KV, receive distance is 20cm to enol/collagen/graphene oxide spinning solution spinning parameter, is promoted
Rate is 1mL/min, and it is 300r/min that roller, which receives revolving speed)；Polyvinyl alcohol/collagen/graphene oxide the spinning solution
Jet stream is generated under electrostatic interaction, receives to obtain nano fiber scaffold on roller；
Step S8: the nano fiber scaffold is subjected to low-temperature vacuum drying (initial temperature is set as -50 DEG C) 48 hours
With use EO mode to carry out sterilization treatment.
In conclusion a kind of preparation method of nano fiber scaffold for promoting wound healing provided by the invention, benefit
The nano fiber scaffold prepared with electrostatic spinning technique, using PVA and collagen as the structure of fiber, effectively simulation natural fine
Extracellular matrix architecture has good mechanical performance, stable physical property and good biocompatibility.In addition, collagen egg
White addition can be used as the substrate of cell attachment and proliferation, and enhancing cell adherence, activity, differentiation and proliferation, GO are further increased
The bioactivity of mixed polymer, to improve adsorption of protein matter.Preparation method of the invention mainly passes through using natural
And the advantages of synthetic polymer and use GO nano particle improve bioactivity, realize the only of the nano fiber scaffold of preparation
Feature.
Above-mentioned preferable possible embodiments only of the invention, are not limitations of the present invention, the present invention is also not limited to above-mentioned
Citing, those skilled in the art, within the essential scope of the present invention, made variations, modifications, additions or substitutions,
Also it should belong to protection scope of the present invention.
Claims (9)
1. a kind of preparation method of the nano fiber scaffold for promoting wound healing, characterized by the following steps:
Step S1: graphene oxide (GO) is prepared using Hummers method；
Step S2: the graphene oxide being dialysed with distilled water, obtains GO sample, the GO sample is collected,
It is placed in and dries in an oven, obtain GO finished product；
Step S3: the GO finished product adds deionized water to be ultrasonically treated, and obtains GO solution；
Step S4: dialysis purification is carried out to collagen, obtains collagen sterling；
Step S5: simultaneously stirring and dissolving is added in deionized water in polyvinyl alcohol (PVA), obtains PVA solution；
Step S6: the collagen sterling and the GO solution are added into the PVA solution, is uniformly mixed, obtains
To polyvinyl alcohol/collagen/graphene oxide spinning solution；
Step S7: polyvinyl alcohol/collagen/graphene oxide spinning solution that step S6 is obtained is placed in electrospinning device
In syringe, apply high-voltage electrostatic field between the spinning head of syringe and the reception device of ground connection, be arranged the polyvinyl alcohol/
Collagen/graphene oxide spinning solution spinning parameter；Polyvinyl alcohol/collagen/graphene oxide the spinning solution exists
Jet stream is generated under electrostatic interaction, obtains nano fiber scaffold in the reception device；
Step S8: the nano fiber scaffold is subjected to vacuum freeze drying and sterilization treatment.
2. the preparation method of the nano fiber scaffold according to claim 1 for promoting wound healing, it is characterised in that:
In the step S2, the dialysis time is 24-48 hours.
3. the preparation method of the nano fiber scaffold according to claim 1 for promoting wound healing, it is characterised in that:
In step S2, the drying condition is 60-65 DEG C, and the drying time is 12-48 hours.
4. the preparation method of the nano fiber scaffold according to claim 1 for promoting wound healing, it is characterised in that:
In step S3, the time of the ultrasonic treatment is 2-10 hours.
5. the preparation method of the nano fiber scaffold according to claim 1 for promoting wound healing, it is characterised in that:
In the step S4, the collagen is the collagen of medical grade, and is Type I collagen albumen, typeⅡ Collagen or III
Collagen type, the time of the dialysis are 24-48 hours.
6. the preparation method of the nano fiber scaffold according to claim 1 for promoting wound healing, it is characterised in that:
In step S5, the mass percentage concentration of the PVA solution is 5%-15%, and the whipping temp condition is 80 DEG C, the stirring
Time is 2-5 hours.
7. the preparation method of the nano fiber scaffold according to claim 1 for promoting wound healing, it is characterised in that:
Quality percentage of the GO in the polyvinyl alcohol/collagen/graphene oxide spinning solution in step S6, in the GO solution
Number is 0.1%-0.5%, quality of the collagen in the polyvinyl alcohol/collagen/graphene oxide spinning solution
Percentage is 2%-10%, and the temperature condition of the stirring is room temperature, and the time of the stirring is 12-24 hours.
8. the preparation method of the nano fiber scaffold according to claim 1 for promoting wound healing, it is characterised in that:
In step S7, the spinning parameter is provided that spinning voltage is 15-30KV, and receiving distance is 10-20cm, and propulsion rate is
0.1-1mL/min, the receiving device are roller, and the revolving speed that receives of the roller is 100-300r/min.
9. the preparation method of the nano fiber scaffold according to claim 1 for promoting skin wound healing, it is characterised in that:
The initial temperature of vacuum freeze drying is set as -50 DEG C to -20 DEG C in step S6, and the time of vacuum freeze drying is 24-48 hours,
Sterilization method is ethylene oxide (EO) sterilizing.
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| CN201811376333.9A CN109453421A (en) | 2018-11-19 | 2018-11-19 | For promoting the preparation method of the nano fiber scaffold of wound healing |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115418064A (en) * | 2022-10-08 | 2022-12-02 | 福建农林大学 | Negative-pressure drainage collagen/GO/PVA composite sponge and preparation method thereof |
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|---|---|---|---|---|
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