CN109444044B - Fertilized egg observation device and working method thereof - Google Patents
Fertilized egg observation device and working method thereof Download PDFInfo
- Publication number
- CN109444044B CN109444044B CN201811297637.6A CN201811297637A CN109444044B CN 109444044 B CN109444044 B CN 109444044B CN 201811297637 A CN201811297637 A CN 201811297637A CN 109444044 B CN109444044 B CN 109444044B
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- China
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- guide sleeve
- air guide
- unit
- fertilized egg
- shell
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides a fertilized egg observation device and a working method thereof, wherein the fertilized egg observation device comprises: an imaging unit including an objective lens module, an imaging device; a delivery unit that delivers the ambient gas to the filter unit; the filtering unit can only allow external gas to enter the space between the shell and the guide cover through the filtering unit, and the space is communicated with the inside of the guide cover through the through hole on the guide cover; the upper part of the air guide sleeve extends into the shell; the inside of the air guide sleeve is hollow; the imaging unit is fixedly connected with the air guide sleeve and keeps sealed; the lower part of the air guide sleeve is provided with taper alpha and at least two circles of through holes communicated with each other, and the number N of the through holes, the diameter R of the through holes and the taper alpha are as follows: pi (30-r·cotα) =1.2n·r; the through holes are respectively positioned inside and outside the shell. The invention has the advantages of small influence of human factors, cleaner and the like.
Description
Technical Field
The invention relates to fertilized eggs, in particular to a fertilized egg observation device and a working method thereof.
Background
At present, a doctor is required to observe the fertilized eggs through a microscope in person, a culture dish of the fertilized eggs is in an open state during the observation of the doctor, and clothes and particles falling off from the skin during the microscopic observation of the doctor can pollute a fertilized egg culture baseband. In addition, through manual microscopic observation of fertilized eggs, doctors need to perform manual focusing, and long-time observation postures can cause fatigue of the doctors, so that judgment of observation results is not facilitated. At present, fertilized egg microscopic observation is carried out on an IVF workstation, the workstation adopts a large-area efficient laminar flow filtering mode in the whole area, a high-power high-rotation-speed fan is adopted for ensuring the laminar flow effect, obvious noise exists in the operation process of the fan, and the noise can influence the operation and misjudgment of doctors. The technology mainly has the following defects:
1. the doctor microscopic observation process has the risk of contaminating the fertilized egg culture medium.
2. At present, a doctor needs to focus in person, and long-time microscopic observation can cause fatigue of the doctor and is unfavorable for judging an observation result.
3. The high-efficiency filtering laminar flow in the whole area can bring more noise, and the utilization rate is very low, but the noise can influence the operation of doctors and bring misjudgment.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides the fertilized egg observation device which has small influence of human factors, no need of manual focusing, clean observation area and small shaking of the surface of a culture medium.
The invention aims at realizing the following technical scheme:
a fertilized egg viewing device, the fertilized egg viewing device comprising:
an imaging unit including an objective lens module, an imaging device;
a delivery unit that delivers the ambient gas to the filter unit;
the filtering unit can only allow external gas to enter the space between the shell and the guide cover through the filtering unit, and the space is communicated with the inside of the guide cover through the through hole on the guide cover;
the upper part of the air guide sleeve extends into the shell;
the inside of the air guide sleeve is hollow; the imaging unit is fixedly connected with the air guide sleeve and keeps sealed; the lower part of the air guide sleeve is provided with taper alpha and at least two circles of through holes communicated with each other, and the number N of the through holes, the diameter R of the through holes and the taper alpha are as follows: pi (30-r·cotα) =1.2n·r; the through holes are respectively positioned inside and outside the shell.
According to the fertilized egg observing apparatus, preferably, the lowermost end of the guide cover is zigzag.
According to the fertilized egg observing apparatus described above, optionally, the transporting unit includes:
the body is annular, an annular groove is formed in the body, and the blades are arranged in the annular groove; the body is fixed on the shell;
the body is provided with an arc through hole; and external air enters the annular groove through the arc-shaped through hole.
According to the fertilized egg observing apparatus described above, preferably, the pod passes through the filter unit and is fixedly connected with the imaging unit.
According to the fertilized egg viewing device described above, optionally, the objective lens module includes a zoom mechanism, and the top end of the pod has a through hole adapted to pass through the zoom mechanism.
According to the fertilized egg observing apparatus described above, optionally, the inner wall of the housing has a projection; the filter unit is located between the projection and the delivery unit and within the housing.
According to the fertilized egg observing apparatus described above, it is preferable that the inner side and the outer side forming the groove press the inner side and the outer side of the annular filter unit, respectively.
According to the fertilized egg observing apparatus described above, preferably, one end of the imaging unit passes through the conveying unit and is kept sealed with the conveying unit.
According to the fertilized egg observing apparatus described above, preferably, the center axis of the through hole is perpendicular to the wall of the pod.
The invention also aims to provide a working method of the fertilized egg observation device, and the aim of the invention is realized by the following technical scheme:
the working method of the fertilized egg observation device comprises the following steps:
(A1) The external gas is sent to the filtering unit through the conveying unit;
(A2) Ambient air can only enter the space between the shell and the air guide sleeve through the filtering unit;
(A3) The gas in the space passes through the through hole of the air guide sleeve and enters the air guide sleeve;
(A4) And the gas in the air guide sleeve passes through the through hole of the air guide sleeve, which is positioned outside the shell, and is discharged.
Compared with the prior art, the invention has the following beneficial effects:
1. the device does not need to manually carry out microscopic observation on fertilized eggs, and reduces the influence of human factors on the culture medium to cause the culture of fertilized eggs;
2. the device omits manual focusing microscopic observation, shortens the observation time and is beneficial to doctors to judge;
3. the efficient laminar flow of the small area enables the microscopic observation area to be cleaner, the cleaning speed is faster, and the noise and vibration are weaker;
4. the uniform wind pressure on the surface of the culture medium is realized, the shaking of the surface of the culture medium is reduced, and the microscopic observation external environment is improved.
Drawings
The present disclosure will become more readily understood with reference to the accompanying drawings. As will be readily appreciated by those skilled in the art: the drawings are only for illustrating the technical scheme of the present invention and are not intended to limit the scope of the present invention. In the figure:
FIG. 1 is a schematic view of a fertilized egg observing apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic illustration of a pod according to an embodiment of the present invention;
FIG. 3 is a schematic view of an applied structure of a fertilized egg observing apparatus according to an embodiment of the present invention.
Detailed Description
Figures 1-3 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and reproduce the invention. In order to teach the technical solution of the present invention, some conventional aspects have been simplified or omitted. Those skilled in the art will appreciate variations or alternatives derived from these embodiments that fall within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Thus, the invention is not limited to the following alternative embodiments, but only by the claims and their equivalents.
Example 1:
fig. 1 schematically shows a schematic configuration diagram of a fertilized egg observing apparatus of embodiment 1 of the present invention, as shown in fig. 1, comprising:
an imaging unit 1 including an objective lens module 11, an imaging device 12, the objective lens module 11 including a zoom mechanism; one end of the imaging unit 1 passes through the body of the conveying unit 2 and keeps sealed with the conveying unit 2;
a delivery unit 2, the delivery unit 2 delivering ambient air to the filtration unit; the conveying unit 2 includes:
the fan comprises a body 21, wherein the body 21 is annular, an annular groove is formed in the body, and blades 22 of the fan are arranged in the annular groove; the body 21 is fixed on the housing 4;
an arc through hole 24, wherein the body 21 is provided with the arc through hole 24; ambient air enters the annular groove through the arc-shaped through holes 24;
the filtering unit 3, such as an annular filter, forms an inner side and an outer side of the annular groove to press the inner side and the outer side of the filtering unit 3 respectively, so that external air can only enter a space between the shell 4 and the guide cover 5 through the filtering unit 3, and the space is communicated with the inside of the guide cover 5 through a through hole 51 on the guide cover 5, thereby ensuring the cleanliness of the air entering the guide cover 5;
a housing 4, wherein the inner wall of the housing 4 is provided with a bulge; the filter unit 3 is located between the projection and the delivery unit 2 and within the housing 4;
a schematic structure of a pod 5 according to embodiment 1 of the present invention is shown in fig. 2, and as shown in fig. 1, the pod 5 is hollow, and has a through hole 53 at the top end for the zoom mechanism to pass through; the upper part of the air guide sleeve 5 stretches into the shell 4, passes through the filtering unit 3 and is fixedly connected with the imaging unit 1 and keeps sealed; the lower part of the air guide sleeve 5 is provided with a taper alpha and at least two circles of through holes 51 communicated with each other, and the number N of the through holes 5, the diameter R of the through holes and the taper alpha are as follows: pi (30-r·cotα) =1.2n·r; the through holes 51 are respectively located inside and outside the housing; the lowest end of the air guide sleeve 5 is in a sawtooth shape 52.
The invention also aims to provide a working method of the fertilized egg observation device, and the aim of the invention is realized by the following technical scheme:
the working method of the fertilized egg observation device comprises the following steps:
(A1) The external gas is sent to the filtering unit through the conveying unit;
(A2) Ambient air can only enter the space between the shell and the air guide sleeve through the filtering unit;
(A3) The gas in the space passes through the through hole of the air guide sleeve and enters the air guide sleeve;
(A4) And the gas in the air guide sleeve passes through the through hole of the air guide sleeve, which is positioned outside the shell, and is discharged.
Example 2:
application example of fertilized egg observation device according to the embodiment of the present invention.
In this application example, as shown in fig. 1 to 2, the imaging device 12 employs a CCD, and the objective lens module 11 employs a zoom mechanism; the outer diameter of the imaging unit 1 is larger than the inner diameter of the main body 21 of the conveying unit 2, and has a through hole 13 adapted for the screw to pass through; the fan adopts a suspension magnetic ring fan, and has no direct contact with the outside, and the noise is low; the outer edge of the body 21 has a through hole 23 adapted for the screw to pass through, and correspondingly the outer wall of the housing 4 also has a hole 41 matching the through hole 23 of the body and adapted for the screw to enter; the filtering unit 3 adopts a high-efficiency annular filter; the central axis of the through hole 51 on the air guide sleeve 5 is perpendicular to the wall of the air guide sleeve 5, and the through hole 53 at the top end of the air guide sleeve 5 is rectangular and is provided with a blind hole 54 matched with the through hole 13 of the imaging unit 1 and suitable for the entry of a screw; the number N of through holes 51 per turn is recommended to be an even number between 14 and 32.
As shown in fig. 3, the culture dish 7 containing fertilized eggs is placed on the culture dish tray 6, and the culture dish tray 6 can be used for placing 6 fertilized egg culture dishes 7 at one time, and can provide backlight for heating and microscopic observation for the fertilized egg culture dishes 7. The area where the culture dish is required to be placed is heated, and after the temperature reaches a preset temperature, the culture dish 7 containing fertilized eggs is placed in the heating area of the culture dish tray 6.
The working method of the fertilized egg observation device of the embodiment is as follows:
(A1) Placing a fertilized egg observation device above the culture dish, and automatically adjusting the backlight intensity of a tray of the culture dish to ensure the microscopic observation light intensity requirement;
the fan starts to work, and the outside air pressurizes the high-efficiency annular filter after passing through the conveying unit;
(A2) Because of the existence of pressure, external gas enters a static pressure cavity formed between the shell and the guide cover through the high-efficiency annular filter;
(A3) The air enters the air guide sleeve through the through hole in the shell, namely the observation cavity, so that the culture dish is ensured to be in a clean environment, and the shaking condition of the culture medium is regulated by regulating the rotating speed of the fan;
(A4) The gas in the air guide sleeve passes through the through hole of the air guide sleeve, which is positioned outside the shell, and is discharged;
(A5) When the internal environment of the observation cavity meets the observation requirement, the imaging unit starts to work, the fertilized eggs in the culture dish are subjected to focusing capture, and the optimal imaging effect is displayed to a doctor for observation through the display. The whole process does not need to be participated by doctors, and only the development condition of the fertilized eggs is evaluated through a display.
Claims (9)
1. A fertilized egg observation device, characterized in that: the fertilized egg observation device includes:
an imaging unit including an objective lens module, an imaging device;
a delivery unit that delivers the ambient gas to the filter unit;
the filtering unit can only allow external gas to enter the space between the shell and the guide cover through the filtering unit, and the space is communicated with the inside of the guide cover through the through hole on the guide cover;
the upper part of the air guide sleeve extends into the shell;
the conveying unit includes: the body is annular, an annular groove is formed in the body, and the blades are arranged in the annular groove; the body is fixed on the shell; the body is provided with an arc through hole; external gas enters the annular groove through the arc-shaped through hole;
the inside of the air guide sleeve is hollow; the imaging unit is fixedly connected with the air guide sleeve and keeps sealed; the lower part of the air guide sleeve is provided with taper alpha and at least two circles of through holes communicated with each other, and the number N of the through holes, the diameter R of the through holes and the taper alpha are as follows: pi (30-r·cotα) =1.2n·r; the through holes are respectively positioned inside and outside the shell;
the working method of the fertilized egg observation device comprises the following steps:
(A1) The external gas is sent to the filtering unit through the conveying unit;
(A2) Ambient air can only enter the space between the shell and the air guide sleeve through the filtering unit;
(A3) The gas in the space passes through the through hole of the air guide sleeve and enters the air guide sleeve;
(A4) And the gas in the air guide sleeve passes through the through hole of the air guide sleeve, which is positioned outside the shell, and is discharged.
2. The fertilized egg viewing device of claim 1, wherein: the lowest end of the air guide sleeve is in a zigzag shape.
3. The fertilized egg viewing device of claim 1, wherein: the air guide sleeve penetrates through the filtering unit and is fixedly connected with the imaging unit.
4. The fertilized egg viewing device of claim 1, wherein: the objective lens module comprises a zooming mechanism, and the top end of the air guide sleeve is provided with a through hole which is suitable for the zooming mechanism to pass through.
5. The fertilized egg viewing device of claim 1, wherein: the inner wall of the shell is provided with a bulge; the filter unit is located between the projection and the delivery unit and within the housing.
6. The fertilized egg viewing device of claim 1, wherein: the inner and outer sides forming the grooves are pressed against the inner and outer sides of the annular filter unit, respectively.
7. The fertilized egg viewing device of claim 1, wherein: one end of the imaging unit passes through the conveying unit and keeps sealed with the conveying unit.
8. The fertilized egg viewing device of claim 1, wherein: the central axis of the through hole is perpendicular to the wall of the air guide sleeve.
9. A method of operating a fertilized egg viewing device as claimed in any one of claims 1 to 8, the method comprising the steps of:
(A1) The external gas is sent to the filtering unit through the conveying unit;
(A2) Ambient air can only enter the space between the shell and the air guide sleeve through the filtering unit;
(A3) The gas in the space passes through the through hole of the air guide sleeve and enters the air guide sleeve;
(A4) The gas in the air guide sleeve passes through the through hole of the air guide sleeve, which is positioned outside the shell, and is discharged;
(A5) When the internal environment of the observation cavity meets the observation requirement, the imaging unit starts to work, the fertilized eggs in the culture dish are captured in a focusing way, and the optimal imaging effect is displayed to a doctor for observation through the display.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2018102553587 | 2018-03-26 | ||
| CN201810255358 | 2018-03-26 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109444044A CN109444044A (en) | 2019-03-08 |
| CN109444044B true CN109444044B (en) | 2023-07-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811297637.6A Active CN109444044B (en) | 2018-03-26 | 2018-11-01 | Fertilized egg observation device and working method thereof |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011004568A1 (en) * | 2009-07-08 | 2011-01-13 | 株式会社ニコン | Image processing method for observation of fertilized eggs, image processing program, image processing device, and method for producing fertilized eggs |
| JP2012039929A (en) * | 2010-08-19 | 2012-03-01 | Nikon Corp | Image processing method, program and apparatus for observing fertilized egg, and method for producing fertilized egg |
-
2018
- 2018-11-01 CN CN201811297637.6A patent/CN109444044B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011004568A1 (en) * | 2009-07-08 | 2011-01-13 | 株式会社ニコン | Image processing method for observation of fertilized eggs, image processing program, image processing device, and method for producing fertilized eggs |
| JP2012039929A (en) * | 2010-08-19 | 2012-03-01 | Nikon Corp | Image processing method, program and apparatus for observing fertilized egg, and method for producing fertilized egg |
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| CN109444044A (en) | 2019-03-08 |
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Effective date of registration: 20190515 Address after: 510700 First Floor, C2 Building, 11 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province Applicant after: GUANGZHOU HUAYUEHANG MEDICAL TECHNOLOGY Co.,Ltd. Applicant after: CHINA INNOVATION INSTRUMENT Co.,Ltd. Address before: Room 304, D Building, Kexin Building, 655 Xueshi Road, Yinzhou District, Ningbo City, Zhejiang Province, 315000 Applicant before: CHINA INNOVATION INSTRUMENT Co.,Ltd. |
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Effective date of registration: 20220216 Address after: 510700 First Floor, C2 Building, 11 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province Applicant after: GUANGZHOU HUAYUEHANG MEDICAL TECHNOLOGY Co.,Ltd. Address before: 510700 First Floor, C2 Building, 11 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province Applicant before: GUANGZHOU HUAYUEHANG MEDICAL TECHNOLOGY Co.,Ltd. Applicant before: CHINA INNOVATION INSTRUMENT Co.,Ltd. |
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