CN103955051B - Viable-tissue-oriented real-time automatic focusing microscopy imaging device and method - Google Patents
Viable-tissue-oriented real-time automatic focusing microscopy imaging device and method Download PDFInfo
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- CN103955051B CN103955051B CN201410199431.5A CN201410199431A CN103955051B CN 103955051 B CN103955051 B CN 103955051B CN 201410199431 A CN201410199431 A CN 201410199431A CN 103955051 B CN103955051 B CN 103955051B
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Abstract
The invention relates to a real-time automatic focusing microscopy imaging device which mainly comprises a microscopy lens, an image collecting module, an image analyzing module, a motor and a motor control module. A real-time automatic focusing method comprises the steps that the image collecting module collects images, the image analyzing module analyzes the definition of the images, and the motor control module controls moving of the motor and the microscopy lens according to the result feedback of the definition until an imaging system obtains images with the best definition. When the relative position of the imaging system and viable tissue changes, the steps are carried out again, and real-time automatic focusing imaging can be achieved. The microscopy lens, the image collecting module, the image analyzing module, the motor and the motor control module are integrated into the same probe, real-time automatic focusing microscopy imaging is achieved, the problem that during a biomedical microscopy imaging process, viable-tissue-oriented dynamic focusing cannot be achieved easily is solved, and operation is simple and practical.
Description
Technical field
The present invention relates to micro imaging system field is and in particular to a kind of automatic focusing in real time towards biological tissue is micro-
Imaging device and method.
Background technology
Micro imaging system is the observation of biomedical microscopic fields and analyzes indispensable instrument, but traditional micro-
Imaging system, needs first to cut off biological tissue from live body, then carries out sealing wax, dehydration, fixing, dyeing, a series of samples such as section
Product set-up procedure, could obtain micro-image.
The optical principle of the micro imaging system of live body is identical with conventional microscope, but sample needs not move through above-mentioned envelope
Wax, dehydration, fixing, dyeing, the complicated set-up procedure such as section, it can need not cut off tissue in its natural state, real to objective body
Existing image checking.The micro imaging system key problem in technology of live body is how to adjust focus so as to overlap with observation position, to obtain
Obtain clearly high-definition picture;Its technological difficulties is, the relative position of position to be imaged and live body micro imaging system is difficult
To keep relative stability, cause system focusing difficult.Existing manual focusing method is when carrying out being imaged for biological tissue, past
Toward requiring operator agile, experienced, it is difficult to real realize manually quick and precisely focusing in practical operation, result is made
Become system imaging to obscure, and be imaged that time-consuming.
Content of the invention
It is an object of the invention to provide structure is simple, be capable of automatic focusing in real time with obtain clear micro-image,
Required image can be carried out with a kind of microscopic imaging device of mug.
A further object of the present invention is to provide a kind of automatic focusing in real time micro imaging method towards biological tissue.
In order to solve above-mentioned technical problem, the invention provides a kind of micro- one-tenth of automatic focusing in real time towards biological tissue
As device, including shell, circuit board, motor, microlens, transparent sealing glass;Described circuit board and motor are arranged on described
Inside the shell is simultaneously fixed on shell;Described transparent sealing glass is arranged on the lower end of described shell;
Described circuit board includes image capture module, image analysis module and motor control module;Described image gathers mould
Block is connected with described image analysis module data wire, and described image analysis module is connected with motor control module data wire;Described
Motor control module is connected with described motor data line, and described motor and described microlens connect;
Described image acquisition module includes sensitive chip and image collection chip.
Further, described sensitive chip is cmos sensitive chip or ccd sensitive chip;In described image acquisition module
Comprise image acquisition procedure, in described image analysis module, comprise image definition evaluation program;The definition of image is according to need
Will be by the shade of gray of image, frequency spectrum or entropy evaluation.
Further, described shell, motor, sensitive chip, microlens and transparent sealing glass are in and are coaxially disposed.
Further, present invention additionally comprises annular light source, described annular light source is arranged at described enclosure and and institute
State shell to be coaxially disposed, described annular light source is arranged on the upper end of described transparent sealing glass;Described annular light source is led annular
Light source or any white light source, monochromater, infrared light supply.
Further, described circuit board is connected with exterior display device data wire.
Further, described motor is vcm voice coil motor or mems microelectromechanical-systems, described motor and described microscope
Head connects;The step-length of described motor is 1 micron -20 microns, and stroke is 10 microns -1000 microns.
Further, described image acquisition module and described microlens composition imaging system, described image acquisition module,
Relative position between microlens, transparent sealing glass is by the parameter determination of microlens so that the focus of imaging system falls
Outer surface in transparent sealing glass.
Further, described shell is provided with button of taking pictures.
Further, described microlens are formed by one piece or several pieces of lens combinations.
A kind of automatic focusing in real time micro imaging method towards biological tissue of the present invention, comprises the following steps:
Step 1: the above-mentioned automatic focusing in real time device towards biological tissue is placed on biological tissue, and makes transparent envelope
Mouth glass gluing needs the biological tissue of imaging.
Step 2: image capture module collection image, the definition of image analysis module analysis of the image, then graphical analyses
Module passes through data line transfer to motor control module the analysis result of definition, and motor control module is clear according to receive
Degree analysis result carrys out feedback control motor movement, thus driving microlens to move.
Step 3: after completing step 2, microlens reach new position, and then circulation step 2, until image clearly.
Step 4: when user sees the picture of needs from external display device, pressing button of taking pictures can make into
As system take needed for picture.
The present invention is used for the micro-imaging of biological tissue, can observe biological tissue in its natural state;During detection, image is adopted
Collection module and image analysis module are constantly in working condition, when biological tissue and apparatus of the present invention relative position change
When, apparatus of the present invention evaluate the definition of image immediately, and when definition is undesirable, motor control module sends signal control
Motor processed and microlens motion, realize automatic focusing in real time function, the present invention can clearly showing with quick obtaining biological tissue
Micro- image;Apparatus of the present invention are easy to use, and are provided with button of taking pictures, and can preserve the image of needs.
Brief description
Fig. 1 is the structural representation of the automatic focusing in real time device of the present invention;
Fig. 2 is the flow chart of auto-focusing of the present invention.
Specific embodiment
Referring to Fig. 1, a kind of automatic focusing in real time microscopic imaging device towards biological tissue of the present invention, including shell 2,
Circuit board 3, motor 4, microlens 5, transparent sealing glass 7;Transparent sealing glass 7 is arranged on the lower end of shell 2;Microlens
5 are formed by one piece or several pieces of lens combinations;Circuit board 3 and motor 4 are arranged in shell 2 and are fixed on shell 2.
Circuit board 3 includes image capture module, image analysis module and motor control module, and three is integrated in same electricity
On the plate of road, image capture module is connected with image analysis module data wire, image analysis module and motor control module data wire
It is connected;Motor 4 is connected by data wire with motor control module;Microlens 5 are connected with motor 4.
Image capture module includes sensitive chip and image collection chip.Sensitive chip is cmos sensitive chip or ccd
Sensitive chip.Setting image definition evaluation program in setting image acquisition procedure, picture analyzing module in image collection chip.
The definition of image as needed by the shade of gray of image, frequency spectrum or entropy evaluation, for focus image definition
Height, has clearly edge and abundant details, and the shade of gray of correspondence image is maximum, and frequency domain high frequency components are most, image
Comentropy maximum.
Present invention additionally comprises annular light source 6, it is arranged inside outer tube 2, and the upper end in transparent sealing glass 7, ring
Shape light source 6 is led annular light source or is any white light source, monochromater, infrared light supply.
Image capture module and the imaging system of microlens 5 this device of composition.Image capture module, microlens 5, thoroughly
Relative position between bright sealing glass 7, by the parameter determination of microlens 5, makes the focus of imaging system fall within transparent during assembling
The outer surface of sealing glass 7.
Motor 4 is vcm voice coil motor or mems microelectromechanical-systems, and the step-length of motor is 1 micron -20 microns, and stroke is
10 microns -1000 microns.
In assembling, the focus of imaging system has been arranged at the outer of transparent sealing glass 7 to imaging system from the above
Surface, and the biological tissue of required imaging and transparent sealing glass 7 are the states of laminating, only need to small range fine tuning during imaging
Can.
Above-mentioned shell 2, motor 4, sensitive chip, microlens 5, annular light source 6 and transparent sealing glass 7 are in coaxially to set
Put.
The circuit board of the present invention passes through data wire and outside display device connects;Present invention additionally comprises being arranged on shell 2
On button 1 of taking pictures, button 1 of taking pictures is used for controlling imaging system to shoot picture, can preserve the image of needs.
Referring to Fig. 2, a kind of real-time automatic focusing method towards biological tissue of the present invention comprises the following steps:
Step 1: the above-mentioned automatic focusing in real time device towards biological tissue is placed on biological tissue, and makes transparent envelope
Mouth glass 7 laminating needs the biological tissue of imaging.
Step 2: image capture module collection image, the definition of image analysis module analysis of the image, then graphical analyses
Module passes through data line transfer to motor control module the analysis result of definition, and motor control module is clear according to receive
Degree analysis result is carried out feedback control motor 4 and is moved, thus driving microlens 5 to move.
Step 3: after completing step 2, microlens 5 reach new position, then circulation step 2, until image clearly.
Step 4: when user sees the picture of needs from external display device, pressing button of taking pictures can make into
As system take needed for picture.
The present invention is used for the micro-imaging of biological tissue, can observe biological tissue in its natural state;During detection, image is adopted
Collection module and image analysis module are constantly in working condition, when biological tissue and apparatus of the present invention relative position change
When, apparatus of the present invention evaluate the definition of image immediately, and when definition is undesirable, motor control module sends signal control
Motor processed and microlens motion, realize automatic focusing in real time function, the present invention can clearly showing with quick obtaining biological tissue
Micro- image;Apparatus of the present invention are easy to use, and are provided with button of taking pictures, and can preserve the image of needs.
The announcement of book and teaching according to the above description, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula is changed and is changed.Therefore, the invention is not limited in specific embodiment disclosed and described above, to the present invention's
In the scope of the claims that some modifications and changes should also be as fall into the present invention.Although additionally, using in this specification
Some specific terms, but these terms are merely for convenience of description, do not constitute any restriction to the present invention.
Claims (9)
1. a kind of automatic focusing in real time microscopic imaging device towards biological tissue it is characterised in that include shell, circuit board,
Motor, microlens, transparent sealing glass;Described circuit board and motor are arranged on described inside the shell and are fixed on shell;Institute
State the lower end that transparent sealing glass is arranged on described shell;
Described circuit board includes image capture module, image analysis module and motor control module;Described image acquisition module and
Described image analysis module data wire is connected, and described image analysis module is connected with motor control module data wire;Described motor
Control module is connected with described motor data line, and described motor is connected with described microlens;
Described image acquisition module includes sensitive chip and image collection chip;
Described image acquisition module and described microlens constitute imaging system, described image acquisition module, microlens, transparent
Relative position between sealing glass is by the parameter determination of microlens so that the focus of imaging system falls within transparent sealing glass
Outer surface.
2. the automatic focusing in real time microscopic imaging device towards biological tissue according to claim 1 is it is characterised in that institute
Stating sensitive chip is cmos sensitive chip or ccd sensitive chip;Image acquisition procedure, institute is comprised in described image acquisition module
State and in image analysis module, comprise image definition evaluation program;The definition of image as needed by the shade of gray of image,
Frequency spectrum or entropy evaluation.
3. the automatic focusing in real time microscopic imaging device towards biological tissue according to claim 1 is it is characterised in that institute
Stating shell, motor, sensitive chip, microlens and transparent sealing glass is in be coaxially disposed.
4. the automatic focusing in real time microscopic imaging device towards biological tissue according to claim 1 is it is characterised in that go back
Including annular light source, described annular light source is arranged at described enclosure and is coaxially disposed with described shell, described ring light
Source is arranged on the upper end of described transparent sealing glass;Described annular light source is any white light source, monochromater, infrared light supply.
5. the automatic focusing in real time microscopic imaging device towards biological tissue according to claim 1 is it is characterised in that institute
State circuit board to be connected with exterior display device data wire.
6. the automatic focusing in real time microscopic imaging device towards biological tissue according to claim 1 is it is characterised in that institute
Stating motor is voice coil motor or microelectromechanical-systems;
The step-length of described motor is 1 micron -20 microns, and stroke is 10 microns -1000 microns.
7. the automatic focusing in real time microscopic imaging device towards biological tissue according to claim 1 is it is characterised in that institute
State and button of taking pictures is provided with shell.
8. the automatic focusing in real time microscopic imaging device towards biological tissue according to claim 1 is it is characterised in that institute
State microlens to be formed by one piece or several pieces of lens combinations.
9. a kind of automatic focusing in real time micro imaging method towards biological tissue is it is characterised in that comprise the following steps:
Step 1: the automatic focusing in real time microscopic imaging device towards biological tissue described in claim 1 is placed in biological tissue
On, and make transparent sealing glass gluing need the biological tissue of imaging;
Step 2: image capture module collection image, the definition of image analysis module analysis of the image, then image analysis module
The analysis result of definition is passed through data line transfer to motor control module, motor control module divides according to the definition receiving
Analysis result feedback control motor movement, thus drive microlens to move;
Step 3: after completing step 2, microlens reach new position, and then circulation step 2, until image clearly;
Step 4: when user sees the picture of needs from external display device, pressing button of taking pictures can make imaging system
System takes required picture.
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CN105785561B (en) * | 2014-12-22 | 2018-04-03 | 杭州舜立光电科技有限公司 | A kind of digital microscope and its focus method |
WO2016101092A1 (en) | 2014-12-22 | 2016-06-30 | 杭州唐光科技有限公司 | Digital microscope and focusing method thereof |
CN109443535A (en) * | 2018-10-30 | 2019-03-08 | 浙江大学 | A kind of underwater microspectrum imaging device and method based on fast illuminated spectrum camera |
CN109669264A (en) * | 2019-01-08 | 2019-04-23 | 哈尔滨理工大学 | Self-adapting automatic focus method based on shade of gray value |
CN110025290A (en) * | 2019-04-22 | 2019-07-19 | 深圳市吉斯迪科技有限公司 | A kind of skin image capture means and its acquisition method |
Citations (4)
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CN2679705Y (en) * | 2003-12-31 | 2005-02-16 | 石家庄钢铁股份有限公司 | Auto focusing device of microscope |
CN103592752A (en) * | 2013-11-18 | 2014-02-19 | 广州博隆兴中信息科技有限公司 | Focus control device and method for living body microscopic imaging system |
CN103760663A (en) * | 2014-01-14 | 2014-04-30 | 宁波基内生物技术有限公司 | Self-adaptive microscope, and use method and self-adaptive focusing method thereof |
CN203838404U (en) * | 2014-05-12 | 2014-09-17 | 广州博隆兴中信息科技有限公司 | Real-time automatic focusing microscopic imaging device for living tissue |
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JP4332905B2 (en) * | 1998-02-12 | 2009-09-16 | 株式会社ニコン | Microscope system |
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CN2679705Y (en) * | 2003-12-31 | 2005-02-16 | 石家庄钢铁股份有限公司 | Auto focusing device of microscope |
CN103592752A (en) * | 2013-11-18 | 2014-02-19 | 广州博隆兴中信息科技有限公司 | Focus control device and method for living body microscopic imaging system |
CN103760663A (en) * | 2014-01-14 | 2014-04-30 | 宁波基内生物技术有限公司 | Self-adaptive microscope, and use method and self-adaptive focusing method thereof |
CN203838404U (en) * | 2014-05-12 | 2014-09-17 | 广州博隆兴中信息科技有限公司 | Real-time automatic focusing microscopic imaging device for living tissue |
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