CN109444044A - Fertilized eggs observe device and its working method - Google Patents

Fertilized eggs observe device and its working method Download PDF

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Publication number
CN109444044A
CN109444044A CN201811297637.6A CN201811297637A CN109444044A CN 109444044 A CN109444044 A CN 109444044A CN 201811297637 A CN201811297637 A CN 201811297637A CN 109444044 A CN109444044 A CN 109444044A
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CN
China
Prior art keywords
pod
hole
fertilized eggs
shell
filter element
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Application number
CN201811297637.6A
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Chinese (zh)
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CN109444044B (en
Inventor
闻路红
励红峰
李文
甘剑勤
郭荣
陈腊
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Guangzhou Huayuehang Medical Technology Co ltd
Original Assignee
Ningbo Huayi Ningchuang Intelligent Science & Technology Co Ltd
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Publication of CN109444044A publication Critical patent/CN109444044A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The present invention provides a kind of fertilized eggs observation device and its working method, the fertilized eggs observation device includes: imaging unit, and the imaging unit includes objective lens module, image device;Ambient atmos are sent to filter element by supply unit, the supply unit;Filter element, ambient atmos are only capable of entering the space between shell and pod by filter element, and the space is connected to by the through-hole on pod with the inside of pod;Shell, the top of pod are protruded into the shell;Pod, the inner hollow of the pod;The imaging unit is fixedly connected with pod and keeps sealing;The lower part of the pod has taper α, and at least two ring through hole with inside and outside connection, and the relationship between the quantity N of each ring through hole, the diameter R of through-hole and taper α is: π (30-Rcot α)=1.2NR;The through-hole is respectively at the inside and outside of the shell.It is small, cleaner that the present invention has many advantages, such as that human factor influences.

Description

Fertilized eggs observe device and its working method
Technical field
The present invention relates to fertilized eggs, in particular to fertilized eggs observation device and its working method.
Background technique
It at present to the observation of fertilized eggs, needs doctor to pass through microscope in person and fertilized eggs is observed, observed in doctor The culture dish of period fertilized eggs is in opening-wide state, the particulate matter meeting fallen on clothing and skin during the microexamination of doctor The risk of pollution is brought to fertilized eggs culture medium.Furthermore by manually carrying out microexamination to fertilized eggs, doctor needs to carry out hand Dynamic focusing, prolonged posture of observing can make doctor generate fatigue, be unfavorable for observing the judgement of result.The micro- sight of fertilized eggs at present Examining is carried out on IVF work station, and work station is using main plot domain large area high efficiency filter laminar flow, to guarantee laminar flow effect It uses the blower of high-power and high-speed, there are obvious noise in blower operation process, noise will affect doctor operation and Erroneous judgement affects.Such technology mainly has following deficiency:
1. there is the risk for bringing pollution to fertilized eggs culture medium in doctor's microexamination process.
2. current microexamination needs doctor to focus in person, prolonged microexamination can make doctor generate fatigue, no Conducive to the judgement of observation result.
3. the high efficiency filter laminar flow in main plot domain can bring bigger noise, and utilization rate is very low, and noise will affect doctor instead Raw operation brings erroneous judgement.
Summary of the invention
To solve the deficiency in above-mentioned prior art, the present invention provides a kind of human factor influence it is small, be not necessarily to people Work focusing, observation area cleaning, media surface shake small fertilized eggs observation device.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of fertilized eggs observation device, the fertilized eggs observation device include:
Imaging unit, the imaging unit include objective lens module, image device;
Ambient atmos are sent to filter element by supply unit, the supply unit;
Filter element, ambient atmos are only capable of entering the space between shell and pod by filter element, the space It is connected to by the through-hole on pod with the inside of pod;
Shell, the top of pod are protruded into the shell;
Pod, the inner hollow of the pod;The imaging unit is fixedly connected with pod and keeps sealing;Institute The lower part for stating pod has taper α, and at least two ring through hole with inside and outside connection, the quantity N of each ring through hole, through-hole Relationship between diameter R and taper α is: π (30-Rcot α)=1.2NR;The through-hole is respectively at the inside of the shell The outside and.
Device is observed according to above-mentioned fertilized eggs, it is preferable that the least significant end of the pod is serrated.
Device is observed according to above-mentioned fertilized eggs, optionally, the supply unit includes:
Ontology, in a ring, inside has annular groove to the ontology, and blade is arranged in the annular groove;Described Body is fixed on the housing;
Arc-shaped through-hole has arc-shaped through-hole on the ontology;Ambient atmos enter the annular groove by arc-shaped through-hole It is interior.
Observe device according to above-mentioned fertilized eggs, it is preferable that the pod passes through the filter element, and with it is described at As unit is fixedly connected.
Device is observed according to above-mentioned fertilized eggs, optionally, the objective lens module includes zoom mechanism, the pod Top, which has, is suitable for the through-hole that the zoom mechanism passes through.
Device is observed according to above-mentioned fertilized eggs, optionally, the outer casing inner wall has protrusion;The filter element is in Between the protrusion and supply unit, and in the shell.
Device is observed according to above-mentioned fertilized eggs, it is preferable that the inner side and outer side for forming the groove is pressing against annular respectively The inner side and outer side of filter element.
Device is observed according to above-mentioned fertilized eggs, it is preferable that one end of the imaging unit passes through the supply unit, and It keeps sealing with the supply unit.
Device is observed according to above-mentioned fertilized eggs, it is preferable that the central axis upright of the through-hole is in the wall of pod.
The object of the invention is also to provide the working methods of above-mentioned fertilized eggs observation device, which is to pass through What following technical scheme was achieved:
According to the working method of above-mentioned fertilized eggs observation device, the working method the following steps are included:
(A1) ambient atmos are sent by supply unit to filter element;
(A2) ambient atmos are only capable of entering the space between shell and pod by the filter element;
(A3) gas in the space passes through the through-hole of pod and enters inside pod;
(A4) gas inside the pod passes through the through-hole discharge outside shell of pod.
Compared with prior art, the device have the advantages that are as follows:
1. the device without artificially to fertilized eggs carry out microexamination, reduce human factor to culture medium bring pollution make by The culture of smart ovum affects;
2. the device removes artificial focusing microexamination from, shorten observing time, is conducive to doctor's judgement;
3. the efficient laminar flow of zonule keeps microexamination region cleaner, and clean speed is more accelerated, noise and vibration It is fainter;
4. realizing the uniform wind pressure of media surface, the shake of media surface is reduced, improves the microexamination external world Environment.
Detailed description of the invention
Referring to attached drawing, the disclosure of the present invention will become more readily understood.Skilled addressee readily understands that: this It is only used to illustrate the technical scheme of the present invention for a little attached drawings, and is not intended to limit the scope of protection of the present invention. In figure:
Fig. 1 is the structure diagram of fertilized eggs observation device according to an embodiment of the present invention;
Fig. 2 is the structure diagram of pod according to an embodiment of the present invention;
Fig. 3 is the application structure schematic diagram of fertilized eggs observation device according to an embodiment of the present invention.
Specific embodiment
Fig. 1-3 and following description describe optional embodiments of the invention to instruct how those skilled in the art implement It is of the invention with reproducing.In order to teach the technical scheme of the invention, it has simplified or omitted some conventional aspects.Those skilled in the art It should be understood that the variation or replacement from these embodiments will within the scope of the invention.Those skilled in the art should understand that The following features can be combined in various ways to form multiple variations of the present invention.As a result, the invention is not limited to it is following can Embodiment is selected, and is only limited by the claims and their equivalents.
Embodiment 1:
Fig. 1 schematically illustrates the structure diagram of the fertilized eggs observation device of the embodiment of the present invention 1, as shown in Figure 1, institute Stating fertilized eggs observation device includes:
Imaging unit 1, the imaging unit include objective lens module 11, image device 12, and the objective lens module 11 includes becoming Focusing mechanism;One end of the imaging unit 1 passes through the ontology of supply unit 2, and keeps sealing with the supply unit 2;
Ambient atmos are sent to filter element by supply unit 2, the supply unit 2;The supply unit 2 includes:
Ontology 21, in a ring, inside has annular groove to the ontology 21, and the setting of blade 22 of blower is recessed in the annular In slot;The ontology 21 is fixed on shell 4;
Arc-shaped through-hole 24 has arc-shaped through-hole 24 on the ontology 21;Ambient atmos pass through described in the entrance of arc-shaped through-hole 24 In annular groove;
Filter element 3, such as annular filter, the inner side and outer side for forming the annular groove is pressing against filter element 3 respectively Inner side and outer side so that ambient atmos are only capable of entering the space between shell 4 and pod 5, the sky by filter element 3 Between be connected to by the through-hole 51 on pod 5 with the inside of pod 5, to guarantee the cleaning of the gas into pod 5 Degree;
Shell 4,4 inner wall of shell have protrusion;The filter element 3 is between the protrusion and supply unit 2, And in the shell 4;
Pod 5, Fig. 2 schematically illustrate the structure diagram of the pod of the embodiment of the present invention 1, as shown in Figure 1, institute The inner hollow of pod 5 is stated, top, which has, is suitable for the through-hole 53 that the zoom mechanism passes through;It stretches on the top of the pod 5 Enter in the shell 4, is fixedly connected with and keeps sealing across filter element 3 and with imaging unit 1;The lower part of the pod 5 With taper α, and at least two ring through hole 51 with inside and outside connection, the quantity N of each ring through hole 5, the diameter R of through-hole and taper Relationship between α is: π (30-Rcot α)=1.2NR;The through-hole 51 is respectively at the inside and outside of the shell;Institute The least significant end for stating pod 5 is serrated 52.
The object of the invention is also to provide the working methods of above-mentioned fertilized eggs observation device, which is to pass through What following technical scheme was achieved:
According to the working method of above-mentioned fertilized eggs observation device, the working method the following steps are included:
(A1) ambient atmos are sent by supply unit to filter element;
(A2) ambient atmos are only capable of entering the space between shell and pod by the filter element;
(A3) gas in the space passes through the through-hole of pod and enters inside pod;
(A4) gas inside the pod passes through the through-hole discharge outside shell of pod.
Embodiment 2:
The application examples of fertilized eggs observation device according to an embodiment of the present invention.
In the application examples, as shown in Figs. 1-2, image device 12 uses CCD, and objective lens module 11 uses zoom mechanism;At As the outer diameter of unit 1 is greater than the internal diameter of the main body 21 of supply unit 2, and has and be suitable for the through-hole 13 that passes through of screw;Blower uses Suspension magnet ring blower, with extraneous without directly contacting, noise is small;The outer of main body 21, which has, is suitable for the through-hole 23 that screw passes through, with This accordingly, the outer wall of shell 4 also have is matched with the through-hole 23 of main body and be suitable for screw enter hole 41;Filter element 3 is adopted With highly effective ring filter;The central axis upright of through-hole 51 on pod 5 leads in the wall of pod 5,5 top of pod Hole 53 is rectangle, and has matched with the through-hole 13 of imaging unit 1 and be suitable for the blind hole 54 that screw enters;Every ring through hole 51 Quantity N recommendation is the even number between 14 to 32.
As shown in figure 3, the culture dish 7 equipped with fertilized eggs is placed on culture dish pallet 6,6 energy one of culture dish pallet Secondary property places 6 fertilized eggs culture dishes 7, can provide the backlight of heating and microexamination for fertilized eggs culture dish 7.Heating needs to put Culture dish 7 equipped with fertilized eggs is placed into the heating of culture dish pallet 6 by the region of culture dish after temperature reaches preset temperature In region.
The working method of the fertilized eggs observation device of the present embodiment are as follows:
(A1) fertilized eggs observation device is placed into above culture dish, automatically adjusts the backlight intensity of culture dish pallet to protect Demonstrate,prove microexamination light intensity demand;
Blower starts to work, and outside air is by pressurizeing to highly effective ring filter after supply unit;
(A2) due to the presence of pressure, ambient atmos are entered between shell and pod by highly effective ring filter to be formed Static pressure chamber;
(A3) gas is entered in pod by the through-hole in shell on pod, namely observation is intracavitary, thus Guarantee that culture dish is in the environment of cleaning, and the big minor adjustment culture medium jitter conditions by adjusting rotation speed of fan;
(A4) gas inside the pod passes through the through-hole discharge outside shell of pod;
(A5) after observing the satisfaction observation requirement of chamber internal environment, imaging unit starts to work, in culture dish Fertilized eggs carry out focusing capture, and optimal imaging effect is observed by display exhibits to doctor.Whole process is not necessarily to Doctor starts to participate in, and need to only be made an appraisal by display to development of fertilized ova situation.

Claims (10)

1. a kind of fertilized eggs observe device, it is characterised in that: the fertilized eggs observe device and include:
Imaging unit, the imaging unit include objective lens module, image device;
Ambient atmos are sent to filter element by supply unit, the supply unit;
Filter element, ambient atmos are only capable of entering the space between shell and pod by filter element, and the space passes through Through-hole on pod is connected to the inside of pod;
Shell, the top of pod are protruded into the shell;
Pod, the inner hollow of the pod;The imaging unit is fixedly connected with pod and keeps sealing;It is described to lead The lower part of stream cover has taper α, and at least two ring through hole with inside and outside connection, the diameter of the quantity N of each ring through hole, through-hole Relationship between R and taper α is: π (30-Rcot α)=1.2NR;The through-hole is respectively at the inside of the shell and outer Portion.
2. fertilized eggs according to claim 1 observe device, it is characterised in that: the least significant end of the pod is in sawtooth Shape.
3. fertilized eggs according to claim 1 observe device, it is characterised in that: the supply unit includes:
Ontology, in a ring, inside has annular groove to the ontology, and blade is arranged in the annular groove;The ontology is solid Determine on the housing;
Arc-shaped through-hole has arc-shaped through-hole on the ontology;Ambient atmos are entered in the annular groove by arc-shaped through-hole.
4. fertilized eggs according to claim 1 observe device, it is characterised in that: it is single that the pod passes through the filtering Member, and be fixedly connected with the imaging unit.
5. fertilized eggs according to claim 1 observe device, it is characterised in that: the objective lens module includes zoom mechanism, The top of the pod, which has, is suitable for the through-hole that the zoom mechanism passes through.
6. fertilized eggs according to claim 1 observe device, it is characterised in that: the outer casing inner wall has protrusion;It is described Filter element is between the protrusion and supply unit, and in the shell.
7. fertilized eggs according to claim 3 observe device, it is characterised in that: form the inner side and outer side point of the groove The inner side and outer side of annular filter unit is not being pressed against.
8. fertilized eggs according to claim 1 observe device, it is characterised in that: one end of the imaging unit passes through described Supply unit, and keep sealing with the supply unit.
9. fertilized eggs according to claim 1 observe device, it is characterised in that: the central axis upright of the through-hole is in leading Flow the wall of cover.
10. the working method of -9 any fertilized eggs observation devices according to claim 1, the working method includes following Step:
(A1) ambient atmos are sent by supply unit to filter element;
(A2) ambient atmos are only capable of entering the space between shell and pod by the filter element;
(A3) gas in the space passes through the through-hole of pod and enters inside pod;
(A4) gas inside the pod passes through the through-hole discharge outside shell of pod.
CN201811297637.6A 2018-03-26 2018-11-01 Fertilized egg observation device and working method thereof Active CN109444044B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2018102553587 2018-03-26
CN201810255358 2018-03-26

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CN109444044A true CN109444044A (en) 2019-03-08
CN109444044B CN109444044B (en) 2023-07-18

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011004568A1 (en) * 2009-07-08 2011-01-13 株式会社ニコン Image processing method for observation of fertilized eggs, image processing program, image processing device, and method for producing fertilized eggs
JP2012039929A (en) * 2010-08-19 2012-03-01 Nikon Corp Image processing method, program and apparatus for observing fertilized egg, and method for producing fertilized egg

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011004568A1 (en) * 2009-07-08 2011-01-13 株式会社ニコン Image processing method for observation of fertilized eggs, image processing program, image processing device, and method for producing fertilized eggs
JP2012039929A (en) * 2010-08-19 2012-03-01 Nikon Corp Image processing method, program and apparatus for observing fertilized egg, and method for producing fertilized egg

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Effective date of registration: 20190515

Address after: 510700 First Floor, C2 Building, 11 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province

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Address before: Room 304, D Building, Kexin Building, 655 Xueshi Road, Yinzhou District, Ningbo City, Zhejiang Province, 315000

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Effective date of registration: 20220216

Address after: 510700 First Floor, C2 Building, 11 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province

Applicant after: GUANGZHOU HUAYUEHANG MEDICAL TECHNOLOGY Co.,Ltd.

Address before: 510700 First Floor, C2 Building, 11 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province

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Applicant before: CHINA INNOVATION INSTRUMENT Co.,Ltd.

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