Fertilized eggs observe device and its working method
Technical field
The present invention relates to fertilized eggs, in particular to fertilized eggs observation device and its working method.
Background technique
It at present to the observation of fertilized eggs, needs doctor to pass through microscope in person and fertilized eggs is observed, observed in doctor
The culture dish of period fertilized eggs is in opening-wide state, the particulate matter meeting fallen on clothing and skin during the microexamination of doctor
The risk of pollution is brought to fertilized eggs culture medium.Furthermore by manually carrying out microexamination to fertilized eggs, doctor needs to carry out hand
Dynamic focusing, prolonged posture of observing can make doctor generate fatigue, be unfavorable for observing the judgement of result.The micro- sight of fertilized eggs at present
Examining is carried out on IVF work station, and work station is using main plot domain large area high efficiency filter laminar flow, to guarantee laminar flow effect
It uses the blower of high-power and high-speed, there are obvious noise in blower operation process, noise will affect doctor operation and
Erroneous judgement affects.Such technology mainly has following deficiency:
1. there is the risk for bringing pollution to fertilized eggs culture medium in doctor's microexamination process.
2. current microexamination needs doctor to focus in person, prolonged microexamination can make doctor generate fatigue, no
Conducive to the judgement of observation result.
3. the high efficiency filter laminar flow in main plot domain can bring bigger noise, and utilization rate is very low, and noise will affect doctor instead
Raw operation brings erroneous judgement.
Summary of the invention
To solve the deficiency in above-mentioned prior art, the present invention provides a kind of human factor influence it is small, be not necessarily to people
Work focusing, observation area cleaning, media surface shake small fertilized eggs observation device.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of fertilized eggs observation device, the fertilized eggs observation device include:
Imaging unit, the imaging unit include objective lens module, image device;
Ambient atmos are sent to filter element by supply unit, the supply unit;
Filter element, ambient atmos are only capable of entering the space between shell and pod by filter element, the space
It is connected to by the through-hole on pod with the inside of pod;
Shell, the top of pod are protruded into the shell;
Pod, the inner hollow of the pod;The imaging unit is fixedly connected with pod and keeps sealing;Institute
The lower part for stating pod has taper α, and at least two ring through hole with inside and outside connection, the quantity N of each ring through hole, through-hole
Relationship between diameter R and taper α is: π (30-Rcot α)=1.2NR;The through-hole is respectively at the inside of the shell
The outside and.
Device is observed according to above-mentioned fertilized eggs, it is preferable that the least significant end of the pod is serrated.
Device is observed according to above-mentioned fertilized eggs, optionally, the supply unit includes:
Ontology, in a ring, inside has annular groove to the ontology, and blade is arranged in the annular groove;Described
Body is fixed on the housing;
Arc-shaped through-hole has arc-shaped through-hole on the ontology;Ambient atmos enter the annular groove by arc-shaped through-hole
It is interior.
Observe device according to above-mentioned fertilized eggs, it is preferable that the pod passes through the filter element, and with it is described at
As unit is fixedly connected.
Device is observed according to above-mentioned fertilized eggs, optionally, the objective lens module includes zoom mechanism, the pod
Top, which has, is suitable for the through-hole that the zoom mechanism passes through.
Device is observed according to above-mentioned fertilized eggs, optionally, the outer casing inner wall has protrusion;The filter element is in
Between the protrusion and supply unit, and in the shell.
Device is observed according to above-mentioned fertilized eggs, it is preferable that the inner side and outer side for forming the groove is pressing against annular respectively
The inner side and outer side of filter element.
Device is observed according to above-mentioned fertilized eggs, it is preferable that one end of the imaging unit passes through the supply unit, and
It keeps sealing with the supply unit.
Device is observed according to above-mentioned fertilized eggs, it is preferable that the central axis upright of the through-hole is in the wall of pod.
The object of the invention is also to provide the working methods of above-mentioned fertilized eggs observation device, which is to pass through
What following technical scheme was achieved:
According to the working method of above-mentioned fertilized eggs observation device, the working method the following steps are included:
(A1) ambient atmos are sent by supply unit to filter element;
(A2) ambient atmos are only capable of entering the space between shell and pod by the filter element;
(A3) gas in the space passes through the through-hole of pod and enters inside pod;
(A4) gas inside the pod passes through the through-hole discharge outside shell of pod.
Compared with prior art, the device have the advantages that are as follows:
1. the device without artificially to fertilized eggs carry out microexamination, reduce human factor to culture medium bring pollution make by
The culture of smart ovum affects;
2. the device removes artificial focusing microexamination from, shorten observing time, is conducive to doctor's judgement;
3. the efficient laminar flow of zonule keeps microexamination region cleaner, and clean speed is more accelerated, noise and vibration
It is fainter;
4. realizing the uniform wind pressure of media surface, the shake of media surface is reduced, improves the microexamination external world
Environment.
Detailed description of the invention
Referring to attached drawing, the disclosure of the present invention will become more readily understood.Skilled addressee readily understands that: this
It is only used to illustrate the technical scheme of the present invention for a little attached drawings, and is not intended to limit the scope of protection of the present invention.
In figure:
Fig. 1 is the structure diagram of fertilized eggs observation device according to an embodiment of the present invention;
Fig. 2 is the structure diagram of pod according to an embodiment of the present invention;
Fig. 3 is the application structure schematic diagram of fertilized eggs observation device according to an embodiment of the present invention.
Specific embodiment
Fig. 1-3 and following description describe optional embodiments of the invention to instruct how those skilled in the art implement
It is of the invention with reproducing.In order to teach the technical scheme of the invention, it has simplified or omitted some conventional aspects.Those skilled in the art
It should be understood that the variation or replacement from these embodiments will within the scope of the invention.Those skilled in the art should understand that
The following features can be combined in various ways to form multiple variations of the present invention.As a result, the invention is not limited to it is following can
Embodiment is selected, and is only limited by the claims and their equivalents.
Embodiment 1:
Fig. 1 schematically illustrates the structure diagram of the fertilized eggs observation device of the embodiment of the present invention 1, as shown in Figure 1, institute
Stating fertilized eggs observation device includes:
Imaging unit 1, the imaging unit include objective lens module 11, image device 12, and the objective lens module 11 includes becoming
Focusing mechanism;One end of the imaging unit 1 passes through the ontology of supply unit 2, and keeps sealing with the supply unit 2;
Ambient atmos are sent to filter element by supply unit 2, the supply unit 2;The supply unit 2 includes:
Ontology 21, in a ring, inside has annular groove to the ontology 21, and the setting of blade 22 of blower is recessed in the annular
In slot;The ontology 21 is fixed on shell 4;
Arc-shaped through-hole 24 has arc-shaped through-hole 24 on the ontology 21;Ambient atmos pass through described in the entrance of arc-shaped through-hole 24
In annular groove;
Filter element 3, such as annular filter, the inner side and outer side for forming the annular groove is pressing against filter element 3 respectively
Inner side and outer side so that ambient atmos are only capable of entering the space between shell 4 and pod 5, the sky by filter element 3
Between be connected to by the through-hole 51 on pod 5 with the inside of pod 5, to guarantee the cleaning of the gas into pod 5
Degree;
Shell 4,4 inner wall of shell have protrusion;The filter element 3 is between the protrusion and supply unit 2,
And in the shell 4;
Pod 5, Fig. 2 schematically illustrate the structure diagram of the pod of the embodiment of the present invention 1, as shown in Figure 1, institute
The inner hollow of pod 5 is stated, top, which has, is suitable for the through-hole 53 that the zoom mechanism passes through;It stretches on the top of the pod 5
Enter in the shell 4, is fixedly connected with and keeps sealing across filter element 3 and with imaging unit 1;The lower part of the pod 5
With taper α, and at least two ring through hole 51 with inside and outside connection, the quantity N of each ring through hole 5, the diameter R of through-hole and taper
Relationship between α is: π (30-Rcot α)=1.2NR;The through-hole 51 is respectively at the inside and outside of the shell;Institute
The least significant end for stating pod 5 is serrated 52.
The object of the invention is also to provide the working methods of above-mentioned fertilized eggs observation device, which is to pass through
What following technical scheme was achieved:
According to the working method of above-mentioned fertilized eggs observation device, the working method the following steps are included:
(A1) ambient atmos are sent by supply unit to filter element;
(A2) ambient atmos are only capable of entering the space between shell and pod by the filter element;
(A3) gas in the space passes through the through-hole of pod and enters inside pod;
(A4) gas inside the pod passes through the through-hole discharge outside shell of pod.
Embodiment 2:
The application examples of fertilized eggs observation device according to an embodiment of the present invention.
In the application examples, as shown in Figs. 1-2, image device 12 uses CCD, and objective lens module 11 uses zoom mechanism;At
As the outer diameter of unit 1 is greater than the internal diameter of the main body 21 of supply unit 2, and has and be suitable for the through-hole 13 that passes through of screw;Blower uses
Suspension magnet ring blower, with extraneous without directly contacting, noise is small;The outer of main body 21, which has, is suitable for the through-hole 23 that screw passes through, with
This accordingly, the outer wall of shell 4 also have is matched with the through-hole 23 of main body and be suitable for screw enter hole 41;Filter element 3 is adopted
With highly effective ring filter;The central axis upright of through-hole 51 on pod 5 leads in the wall of pod 5,5 top of pod
Hole 53 is rectangle, and has matched with the through-hole 13 of imaging unit 1 and be suitable for the blind hole 54 that screw enters;Every ring through hole 51
Quantity N recommendation is the even number between 14 to 32.
As shown in figure 3, the culture dish 7 equipped with fertilized eggs is placed on culture dish pallet 6,6 energy one of culture dish pallet
Secondary property places 6 fertilized eggs culture dishes 7, can provide the backlight of heating and microexamination for fertilized eggs culture dish 7.Heating needs to put
Culture dish 7 equipped with fertilized eggs is placed into the heating of culture dish pallet 6 by the region of culture dish after temperature reaches preset temperature
In region.
The working method of the fertilized eggs observation device of the present embodiment are as follows:
(A1) fertilized eggs observation device is placed into above culture dish, automatically adjusts the backlight intensity of culture dish pallet to protect
Demonstrate,prove microexamination light intensity demand;
Blower starts to work, and outside air is by pressurizeing to highly effective ring filter after supply unit;
(A2) due to the presence of pressure, ambient atmos are entered between shell and pod by highly effective ring filter to be formed
Static pressure chamber;
(A3) gas is entered in pod by the through-hole in shell on pod, namely observation is intracavitary, thus
Guarantee that culture dish is in the environment of cleaning, and the big minor adjustment culture medium jitter conditions by adjusting rotation speed of fan;
(A4) gas inside the pod passes through the through-hole discharge outside shell of pod;
(A5) after observing the satisfaction observation requirement of chamber internal environment, imaging unit starts to work, in culture dish
Fertilized eggs carry out focusing capture, and optimal imaging effect is observed by display exhibits to doctor.Whole process is not necessarily to
Doctor starts to participate in, and need to only be made an appraisal by display to development of fertilized ova situation.