CN109439675A - Plant disease-resistant related gene RLK902 and its application - Google Patents

Plant disease-resistant related gene RLK902 and its application Download PDF

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CN109439675A
CN109439675A CN201811584229.9A CN201811584229A CN109439675A CN 109439675 A CN109439675 A CN 109439675A CN 201811584229 A CN201811584229 A CN 201811584229A CN 109439675 A CN109439675 A CN 109439675A
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rlk902
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唐定中
赵耀飞
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Fujian Agriculture and Forestry University
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    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance

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Abstract

The present invention relates to plant disease-resistant related genesRLK902(RECEPTOR LIKE KINASE 902) and application.The method that the present invention passes through interaction protein screening, has found one and vegetative bacteria venereal disease opportunistic pathogen resistance related geneRLK902And Function Identification has been carried out, it finds and proves for the first timeRLK902Gene, to the positive regulating and controlling effect in pseudomonas syringae resistance, is improved in plantRLK902Gene expression dose can significantly increase the disease resistance of plant.Present invention could apply to improve the initiative of the genetically modified crops of crop resistance etc..

Description

Plant disease-resistant related gene RLK902 and its application
Technical field
The present invention relates to plant disease-resistant related genesRLK902And its application.Pass through the sieve of EDR4 interaction protein Choosing, we have found a leucine enrichment receptor albuminoid kinases, this kinases byRLK902Gene coding, whenRLK902 When the deletion mutation of gene and expression quantity are suppressed, plant significantly reduces the disease resistance of pseudomonas syringae, and mentions It is highRLK902Gene expression dose can be enhanced plant to the disease resistance of pseudomonas syringae and promote in plant resist The expression of sick related gene, belongs to genetic engineering field.
Background technique
Bacterial pathogen is a kind of important plant disease, and pathogenetic bacteria is mostly rod bacterium, and main includes five categories: false Pseudomonas bacillus category (Pseudomonas), Xanthomonas (Xanthomonas), erwinia category (Erwinia), wild bar Pseudomonas (Agrobacterium) and Corynebacterium (Corynebacterium).They have one or several flagellum mostly, can To be propagated by nature hole (stomata, hole skin, water hole) or wound instruction plant, and by media such as rainwater, mediator insects.It invades After contaminating plant, it is even downright bad and rotten to will cause plant leaf blade wilting.Bacterial pathogen can infect diversified economy crop, make At huge economic loss, bacterial leaf spot disease, the knot that European Bacillus induces can be caused as Xanthomonas infects rice Cabbage soft rot, Corynebacterium infect bacterial canker etc. caused by tomato.The prevention and treatment of bacterial pathogen can generally lead to Seedling drug disinfection, prevention and treatment mediator insect and initiative disease-resistant variety are crossed to realize, more disease-resistant related genes are excavated in research And its anti-disease mechanism, genetic resources and theoretical basis can be provided for the breeding of disease-resistant variety.
In pathogen instruction plant, plant can resist the invasion of pathogen by natural immune system.Plant basis Resistance is the important component of natural plant immune system.Basal resistance is the pattern receptors basis by being located on cell membrane Resistance identifies pathogen phase by the pattern receptors (Pattern Recognition Receptor, PRR) being located on cell membrane It closes molecular pattern (pathogen-associated molecular patterns, PAMPs), passes through the shape of Receptor Complex At with activation, and the caused immune response of signal transmitting in dependent cells, the immune response of also referred to as PAMPs induction (PAMPs triggered immunity, PTI) is the first level of defense response.Receptor class kinases in plant (Receptor like kinases, RLKs) is a most important albuminoid, this albuminoid in pattern receptors complex member The identification of pathogen associated molecular pattern can directly be participated in and activation that early immune reacts.
Early period the study found that arabidopsis EDR4 albumen takes part in the vesicle transport process of plant disease resistance-related protein, lead to Interaction albumen of the research EDR4 in arabidopsis is crossed, more participation plant immune regulatory factors can be excavated.We pass through firefly Fiery element enzyme complementation imaging experiment, a variety of methods such as co-immunoprecipitation determined one byRECEPTOR LIKE KINASE 902 (RLK902) coding receptor class kinases and EDR4 in plant exist interaction.RLK902After mutation, arabidopsis is for fourth The disease resistance of fragrant Pseudomonas alba weakens, and is improved by transgenosisRLK902Gene expression dose can significantly increase plant To the disease resistance of pseudomonas syringae.We are using arabidopsis and pseudomonas syringae as research system, by rightRLK902 Functional analysis, find that the disease resistance response of the gene pairs plant against bacterial pathogen plays an important role for the first time.We The result shows thatRLK902It is the good candidate gene for the genetically modified crops and crop MOLECULE DESIGN for creating disease resistance enhancing, there is weight The theoretical value and wide application prospect wanted.
Summary of the invention
The purpose of the present invention is to provide plant disease-resistant related genesRLK902And its application.
To achieve the above object, the present invention adopts the following technical scheme:
1. an arabidopsis disease-resistant geneRLK902Sequence, 1944 bp of CDS overall length.Its CDS sequence such as SEQ ID NO.1 institute Show.
2.RLK902 protein sequence encodes 647 amino acid, 70.41 Kd of size.Its amino acid sequence such as SEQ ID Shown in NO.2.
3.RLK902Arabidopsis is reduced to the disease resistance of pseudomonas syringae after mutation, and is improvedRLK902Base Because expression can significantly increase plant to the disease resistance of pseudomonas syringae.
4. raising can be passed through by technique for gene engineeringRLK902Gene expression dose carrys out the disease resistance of Crop Improvement Shape.
The present invention has the advantages that
Applicant by Luciferase complementation imaging experiment, a variety of methods such as co-immunoprecipitation determined one byRECEPTOR LIKE KINASE 902(RLK902) coding receptor class kinases and EDR4 in plant exist interaction.RLK902Mutation Afterwards, arabidopsis weakens the disease resistance of pseudomonas syringae, and is improved by transgenosisRLK902Gene expression dose Plant can be significantly increased to the disease resistance of pseudomonas syringae.We are research body with arabidopsis and pseudomonas syringae System, by rightRLK902Functional analysis, find for the first time the gene pairs plant against bacterial pathogen disease resistance response have weight The effect wanted.Our result indicate thatRLK902Be create disease resistance enhancing genetically modified crops and crop MOLECULE DESIGN it is excellent Candidate gene has important theoretical value and wide application prospect.
Detailed description of the invention
Fig. 1 is detection RLK902 and EDR4 interaction diagram, wherein A be detected using yeast two-hybrid RLK902 with There is interaction in EDR4；B be in tobacco Transient Expression System, by Luciferase complementation imaging experiment detect RLK902 with Interaction of the EDR4 in plant；C is to construct the quasi- south of transgenosis for stablizing expression RLK902 and EDR4 by transgenic technology Mustard, interaction of the RLK902 and EDR4 in plant in the case where stablizing expression by co-immunoprecipitation detection.
Fig. 2 isrlk902Mutant enhancing is to pseudomonas syringae susceptibility figure, and wherein A isRLK902Gene andrlk902The T-DNA insertion mutation position view of mutant；B is arabidopsis WT lines Col-0,rlk902Mutant,gRLK902/rlk902Genetic complement plant is inoculated with pseudomonas syringaePtoBacterium colony count results after DC3000, C are quasi- south Mustard WT lines Col-0,rlk902Mutant,gRLK902/rlk902Genetic complement plant is inoculated with pseudomonas syringaePto DC3000 hrcC - Bacterium colony count results afterwards, D are inoculationPtoDisease-resistant related gene after DC3000PR1Gene expression water Flat detection, E are inoculationPtoDisease-resistant related gene after DC3000PR2Gene expression dose detection.
Fig. 3 is that RLK902 enhancing is overexpressed in arabidopsis to pseudomonas syringae resistance figure, and wherein A is not connect bacterium plant The growth phenotype of object is taken pictures after short photoperiod grows four weeks, scale=1 cm.B is to detect table by real-time quantitative PCR Up in plantRLK902Gene expression dose.C is southern mustard WT lines Col-0,rlk902Mutant,gRLK902/rlk902 Genetic complement plant and overexpressionRLK902Transgenic plant be inoculated with pseudomonas syringaePtoBacterium colony meter after DC3000 Number is as a result, D is inoculationPtoDisease-resistant related gene after DC3000PR1Gene expression dose detection, E be inoculationPto Disease-resistant related gene after DC3000PR2Gene expression dose detection.
Specific embodiment
Embodiment one is tested by interactions between protein candidate albumen RLK902 being confirmed as goal in research
(1) materials and methods
It will be expanded using the two-hybrid system of yeast strain AH109 using the cDNA of arabidopsis wild type Col-0 as template It arrivesEDR4WithRLK902Overall length CDS sequence is connected into pGADT7 (AD) and pGBKT7 (BD) carrier, and corresponding yeast is double Hybrid vector cotransformation yeast, and use pGADT7 (AD) and pGBKT7 (BD) empty carrier as control, yeast exists after double conversions Defect synthetic dropout (SD) culture medium SD-Trp/-Leu(detection plasmid is double to turn positive transformants yeast) and SD- Trp/-Leu/-His/-Ade(detects that same plasmid is double to turn two interactions between protein situations in positive transformants yeast) on grown, It takes pictures after 5 days.
The overall length CDS sequence of EDR4 and RLK902 is connected into pCambia1300-Nluc and pCambia1300-Cluc building Plant expression vector needed for Luciferase complementation imaging experiment simultaneously converts Agrobacterium GV3101, and Ben's tobacco is at 22 DEG C, 9 hours In the greenhouse of illumination, grow 4 weeks.It will carry the Agrobacterium jet injection transformation of tobacco of respective carrier, low light culture 24 hours Then normal illumination culture 48 hours afterwards carry out chemiluminescence and take pictures.
EDR4 and RLK902 full-length genome DNA sequence dna with own promoter sequence is constructed respectively to pMDC107 In pEarLeyGate301 carrier, RLK902-GFP the and EDR4-HA expression vector of own promoter driving is obtained, conversion is quasi- Southern mustard simultaneously obtains stable Transgenic wheat line by screening, by transgenic arabidopsis in the greenhouse in 22 DEG C, illumination in 9 hours In, it grows 4 weeks, extracts plant total protein and carry out co-immunoprecipitation experiment, detect protein-interacting.
(2) result and analysis
From the point of view of the result of yeast two-hybrid, RLK902 and EDR4 are co-expressed in yeast can make yeast in four scarce culture mediums Upper growth, and self-activation can't occur for single expression, illustrate that RLK902 and EDR4 has interaction (Fig. 1 A).
By Luciferase complementation imaging experiment and co-immunoprecipitation experiment, it may detect that RLK902's and EDR4 is mutual Make signal, illustrates that RLK902 and EDR4 have interaction under conditions of tobacco transient expression and arabidopsis stablize expression, fill The combination of clear two albumen of defending oneself is existing (Fig. 1 B-C) in plant.
Embodiment tworlk902Mutant is inoculated with pseudomonas syringaePtoThe phenotypic analysis of DC3000
(1) materials and methods
By arabidopsis WT lines Col-0,rlk902Mutant (Arabidopsis Biological Resource The T-DNA insertion mutation that Center is ordered, attached drawing 2A),gRLK902/rlk902It is (above-mentionedRLK902Own promoter driving The pMDC107 expression vector of RLK902 genomic dna sequence convertsrlk902The quasi- south of the stable genetic complement that mutant obtains Mustard plant) andnpr1Mutant (reported susceptibility enhances mutant, is used as susceptible control) is planted in 22 DEG C, 9 small time According to greenhouse in, grow 4 weeks, in our current research as susceptible control.By pseudomonas syringae (PtoDC3000 andPto DC3000 hrcC -) 12 h of scribing line culture on the KB solid medium containing rifampin (Rif) resistance, with the MgCl of 10 mM2 The bacterium grown on culture medium is collected, is OD by gradient dilution to concentration600=5 × 10-4.Bacterial injections are big by 4 weeks The small Arabidopsis leaf back side samples after injecting 3 h, as control in 0 day (0 DPI).Blade is taken with punch.It is added 1 The 10 mM MgCl of mL2, it is fully ground blade, resulting blade homogenate is diluted to 10-1With 10-2It is flat to be applied to KB for two concentration Plate after 28 DEG C are cultivated 2-3 days, counts the bacterium colony on culture medium.Blade after infecting 3 days is sampled with punch again, The 10 mM MgCl of 1 mL are added2, it is fully ground blade, resulting blade homogenate is diluted to 10-4With 10-5Two concentration apply In KB plate, 28 DEG C after culture 3 days (3 DPI), are counted the bacterium colony on culture medium.
With the above-mentioned method inoculation for connecing bacteriumPtoDC3000 took blade at 0 day and 3 days respectively, extracted total serum IgE, reverse transcription CDNA is obtained, disease-resistant related gene is detected by real-time quantitative PCRPR1WithPR2Gene expression dose variation.
(2) result and analysis
InoculationPtoAfter DC3000 tri- days,rlk902The growth of pathogenic bacteria quantity of mutant is significantly higher than wild type, genetic complement StraingRLK902/rlk902Growth of pathogenic bacteria quantity can be restored to the level similar with wild type (Fig. 2 B).Similarly, It is inoculated with three type excretory system deficient strainsPtoDC3000 hrcC - ,rlk902Mutant also shows the susceptibility of enhancing, loses Pass complementary plantgRLK902/rlk902Growth of pathogenic bacteria quantity can be restored to wild-type levels (Fig. 2 C).
Pathogenic bacterium inducingPRThe testing result of gene expression amount variation shows in inoculation pseudomonas syringaePto After DC3000 tri- days,rlk902In mutantPR1WithPR2Transcriptional level can be significantly lower than wild type, genetic complement straingRLK902/rlk902'sPRGene expression amount is similar with wild type (Fig. 2 D-E).Above-mentioned phenotype illustrates RLK902 positive regulation Resistance of the plant to pseudomonas syringae.
The transgenic arabidopsis of three overexpression RLK902 of embodiment is inoculated with pseudomonas syringaePtoThe table of DC3000 Property analysis
(1) materials and methods
Above-mentioned RLK902 overall length CDS sequence is connected into pEarleyGate102 carrier, the RLK902 of 35S promoter driving is obtained Over-express vector, arabidopsis thaliana transformation are screened to obtain Transgenic wheat line by herbicide basta, be examined by real-time quantitative PCR It surveys, is significantly improvedRLK902The transgenic line of gene expression dose.
By wild type Col-0,rlk902Mutant,gRLK902/rlk902Genetic complement plant andRLK902It is overexpressed Plant is planted in 22 DEG C, in the greenhouse of illumination in 9 hours, grows 4 weeks.By pseudomonas syringae (PtoDC3000) containing advantageous good fortune Scribing line culture 12 hours on the KB solid medium of flat (Rif) resistance, with the MgCl of 10 mM2What collection was grown on culture medium Bacterium is OD by gradient dilution to concentration600=5 × 10-4.By bacterial injections to the Arabidopsis leaf back side of 4 weeks sizes, 3 It is sampled after it with punch, the 10 mM MgCl of 1000 μ l is added2, it is fully ground blade, resulting blade homogenate is diluted to 10-4With 10-5Two concentration, are applied to KB plate, and 28 DEG C after culture 3 days (3 DPI), count the bacterium colony on culture medium.
The Arabidopsis leaf for not connecing bacterium processing is taken, total serum IgE is extracted, reverse transcription obtains cDNA, detected by real-time quantitative PCR Disease-resistant related genePR1WithPR2Gene expression dose under different genetic background.
(2) result and analysis
Under normal condition growth, the plant for being overexpressed strain, which shows, grows growth short and small and with spontaneous cell death Phenotype (Fig. 3 A), by quantitative PCR detection, the two overexpressions that we obtainRLK902Transgenic plant OE-1 and The gene expression dose of OE-2, RLK902 are significantly higher than wild-type plant (Fig. 3 B).InoculationPtoAfter DC3000, it is overexpressed strain Bacterium colony growth population be substantially less than wild type, illustrate overexpressionRLK902Plant can be enhanced to pseudomonas syringae Resistance (Fig. 3 C).
We are detected by real-time quantitative PCR in the case where not connecing bacterium simultaneously, and RLK902 is overexpressed in plantPR1WithPR2Gene expression dose be also significantly greater than wild type (Fig. 3 D-E), composition improve disease-resistant related gene expression can Can be overexpressedRLK902One of the reason of improving disease resistance of plant.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification, is all covered by the present invention.
SEQUENCE LISTING
<110>University Of Agriculture and Forestry In Fujian
<120>plant disease-resistant related gene RLK902 and its application
<130> 2
<160> 2
<170> PatentIn version 3.3
<210> 1
<211> 1944
<212> DNA
<213>artificial sequence
<400> 1
atgcgactct tcttcacacc gtcaatgtcc aatctctcca tattcttctc gattcttctc 60
ctttctcttc ctcttccgtc aatcggagat ctcgccgccg acaaatccgc tcttctctct 120
tttcgttccg ccgtcggtgg tcgtacatta ctctgggacg tcaagcaaac ctcaccatgc 180
aactggaccg gcgtcttatg cgacggtggt cgtgttactg ctcttcgtct tcccggtgaa 240
acgctctccg gtcatatacc ggagggtatt tttggtaatt taactcagct ccggacgctt 300
agtctccgtc tcaatggtct tactggttct cttcctttgg atctcggaag ctgctccgat 360
cttcggcgtt tgtacctgca gggtaacaga ttctccggtg agattccgga ggttttgttt 420
agtcttagta accttgttag gttgaatcta gctgagaatg aatttagtgg agagatctcg 480
tcagggttta aaaaccttac taggcttaag actctgtacc tggagaataa caagctctct 540
ggctctcttt tagacttgga tttgtctttg gatcagttca acgtttctaa taacttgttg 600
aacggatcta tacctaagag tttgcagaag tttgattctg attcgtttgt gggaacttct 660
ctctgcggca aaccgcttgt tgtctgctct aatgagggaa ctgtgccaag ccagccaatt 720
tctgttggca atattcccgg aactgttgaa ggaagtgagg agaagaagaa aaggaagaag 780
ctttctggtg gagctatagc tggaatagtg attggatgtg tggttggttt gtccctgatt 840
gttatgattt tgatggttct ctttaggaaa aaggggaacg agagaacaag ggccattgac 900
cttgcaacca tcaagcacca tgaagttgaa attcctggcg agaaagcggc cgtggaagca 960
ccggagaata ggagctatgt aaatgagtac tctccgtctg cagtgaaagc tgtggaagtg 1020
aacagttcag ggatgaagaa gttagtgttt tttgggaatg cgacaaaggt cttcgatctt 1080
gaggatctgt tgagagcttc agcggaggtt ctggggaaag gaacgttcgg gacagcttat 1140
aaagcggtgc ttgacgcggt gacattggtg gctgtgaaga gactgaagga tgtaacgatg 1200
gcggacagag agtttaagga gaagattgag gttgttgggg cgatggatca tgagaacttg 1260
gtgcccttga gagcgtacta ttacagtgga gacgagaagc tgcttgtcta tgacttcatg 1320
cctatgggaa gcttatcagc tctcttacac ggaaacaaag gtgcaggccg gcctccattg 1380
aactgggaag tcagatcagg catcgccctt ggagctgctc gtggcttaga ctatcttcac 1440
tcacaagacc cactgagctc tcacggaaac gtcaagtcct ccaatatcct cttaacaaac 1500
tcccatgacg cacgagtgtc tgatttcggc ctggctcagc ttgtaagcgc ctcatccaca 1560
accccaaacc gggccactgg gtaccgtgcg ccagaagtaa ctgacccgag gcgtgtctca 1620
cagaaagcgg acgtgtacag ctttggtgtg gtgttgctag agttgctcac cggaaaagct 1680
ccgtctaact cggtgatgaa cgaggaagga atggatttgg cgaggtgggt gcattcagtg 1740
gcgagagagg agtggaggaa tgaggttttt gactcggagc tgatgagtat cgagacagtt 1800
gtctcggtgg aagaagagat ggcggaaatg ctgcagctgg gcattgactg tacagagcag 1860
cacccagaca agcggccagt tatggtggag gtggtgagaa ggatccagga gttgcgccaa 1920
tcgggtgcag atcgggtggg gtaa 1944
<210> 2
<211> 647
<212> PRT
<213>artificial sequence
<400> 2
Met Arg Leu Phe Phe Thr Pro Ser Met Ser Asn Leu Ser Ile Phe Phe
1 5 10 15
Ser Ile Leu Leu Leu Ser Leu Pro Leu Pro Ser Ile Gly Asp Leu Ala
20 25 30
Ala Asp Lys Ser Ala Leu Leu Ser Phe Arg Ser Ala Val Gly Gly Arg
35 40 45
Thr Leu Leu Trp Asp Val Lys Gln Thr Ser Pro Cys Asn Trp Thr Gly
50 55 60
Val Leu Cys Asp Gly Gly Arg Val Thr Ala Leu Arg Leu Pro Gly Glu
65 70 75 80
Thr Leu Ser Gly His Ile Pro Glu Gly Ile Phe Gly Asn Leu Thr Gln
85 90 95
Leu Arg Thr Leu Ser Leu Arg Leu Asn Gly Leu Thr Gly Ser Leu Pro
100 105 110
Leu Asp Leu Gly Ser Cys Ser Asp Leu Arg Arg Leu Tyr Leu Gln Gly
115 120 125
Asn Arg Phe Ser Gly Glu Ile Pro Glu Val Leu Phe Ser Leu Ser Asn
130 135 140
Leu Val Arg Leu Asn Leu Ala Glu Asn Glu Phe Ser Gly Glu Ile Ser
145 150 155 160
Ser Gly Phe Lys Asn Leu Thr Arg Leu Lys Thr Leu Tyr Leu Glu Asn
165 170 175
Asn Lys Leu Ser Gly Ser Leu Leu Asp Leu Asp Leu Ser Leu Asp Gln
180 185 190
Phe Asn Val Ser Asn Asn Leu Leu Asn Gly Ser Ile Pro Lys Ser Leu
195 200 205
Gln Lys Phe Asp Ser Asp Ser Phe Val Gly Thr Ser Leu Cys Gly Lys
210 215 220
Pro Leu Val Val Cys Ser Asn Glu Gly Thr Val Pro Ser Gln Pro Ile
225 230 235 240
Ser Val Gly Asn Ile Pro Gly Thr Val Glu Gly Ser Glu Glu Lys Lys
245 250 255
Lys Arg Lys Lys Leu Ser Gly Gly Ala Ile Ala Gly Ile Val Ile Gly
260 265 270
Cys Val Val Gly Leu Ser Leu Ile Val Met Ile Leu Met Val Leu Phe
275 280 285
Arg Lys Lys Gly Asn Glu Arg Thr Arg Ala Ile Asp Leu Ala Thr Ile
290 295 300
Lys His His Glu Val Glu Ile Pro Gly Glu Lys Ala Ala Val Glu Ala
305 310 315 320
Pro Glu Asn Arg Ser Tyr Val Asn Glu Tyr Ser Pro Ser Ala Val Lys
325 330 335
Ala Val Glu Val Asn Ser Ser Gly Met Lys Lys Leu Val Phe Phe Gly
340 345 350
Asn Ala Thr Lys Val Phe Asp Leu Glu Asp Leu Leu Arg Ala Ser Ala
355 360 365
Glu Val Leu Gly Lys Gly Thr Phe Gly Thr Ala Tyr Lys Ala Val Leu
370 375 380
Asp Ala Val Thr Leu Val Ala Val Lys Arg Leu Lys Asp Val Thr Met
385 390 395 400
Ala Asp Arg Glu Phe Lys Glu Lys Ile Glu Val Val Gly Ala Met Asp
405 410 415
His Glu Asn Leu Val Pro Leu Arg Ala Tyr Tyr Tyr Ser Gly Asp Glu
420 425 430
Lys Leu Leu Val Tyr Asp Phe Met Pro Met Gly Ser Leu Ser Ala Leu
435 440 445
Leu His Gly Asn Lys Gly Ala Gly Arg Pro Pro Leu Asn Trp Glu Val
450 455 460
Arg Ser Gly Ile Ala Leu Gly Ala Ala Arg Gly Leu Asp Tyr Leu His
465 470 475 480
Ser Gln Asp Pro Leu Ser Ser His Gly Asn Val Lys Ser Ser Asn Ile
485 490 495
Leu Leu Thr Asn Ser His Asp Ala Arg Val Ser Asp Phe Gly Leu Ala
500 505 510
Gln Leu Val Ser Ala Ser Ser Thr Thr Pro Asn Arg Ala Thr Gly Tyr
515 520 525
Arg Ala Pro Glu Val Thr Asp Pro Arg Arg Val Ser Gln Lys Ala Asp
530 535 540
Val Tyr Ser Phe Gly Val Val Leu Leu Glu Leu Leu Thr Gly Lys Ala
545 550 555 560
Pro Ser Asn Ser Val Met Asn Glu Glu Gly Met Asp Leu Ala Arg Trp
565 570 575
Val His Ser Val Ala Arg Glu Glu Trp Arg Asn Glu Val Phe Asp Ser
580 585 590
Glu Leu Met Ser Ile Glu Thr Val Val Ser Val Glu Glu Glu Met Ala
595 600 605
Glu Met Leu Gln Leu Gly Ile Asp Cys Thr Glu Gln His Pro Asp Lys
610 615 620
Arg Pro Val Met Val Glu Val Val Arg Arg Ile Gln Glu Leu Arg Gln
625 630 635 640
Ser Gly Ala Asp Arg Val Gly
645

Claims (4)

1. plant disease-resistant related geneRLK902,It is characterized by: the geneRLK9021944 bp of CDS overall length, CDS sequence As shown in SEQ ID NO.1.
2. the plant disease-resistant related geneRLK902The albumen of coding, amino acid sequence is as shown in SEQ ID NO.2.
3. the plant disease-resistant related geneRLK902Improving the application in disease resistance of plant.
4. the plant disease-resistant related geneRLK902Application in anti-pseudomonas syringae.
CN201811584229.9A 2018-12-24 2018-12-24 Plant disease-resistant related gene RLK902 and its application Pending CN109439675A (en)

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CN117646028A (en) * 2023-10-20 2024-03-05 中国农业科学院油料作物研究所 Application of knockout BnaA05.RLK902 gene in improvement of plant sclerotinia resistance and gray mold and molecular marker

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CN113151320A (en) * 2021-03-22 2021-07-23 华中农业大学 Potato StLecRK-VI.1 and StTET8 genes and application thereof in improvement of late blight resistance
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CN113832124A (en) * 2021-10-19 2021-12-24 武夷学院 Application of protein-related biological material in enhancing resistance to bacterial leaf blight of rice
CN117646028A (en) * 2023-10-20 2024-03-05 中国农业科学院油料作物研究所 Application of knockout BnaA05.RLK902 gene in improvement of plant sclerotinia resistance and gray mold and molecular marker

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Application publication date: 20190308