CN109439646B - Microorganism immobilized bio-based material and preparation method and application thereof - Google Patents
Microorganism immobilized bio-based material and preparation method and application thereof Download PDFInfo
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- CN109439646B CN109439646B CN201811378868.XA CN201811378868A CN109439646B CN 109439646 B CN109439646 B CN 109439646B CN 201811378868 A CN201811378868 A CN 201811378868A CN 109439646 B CN109439646 B CN 109439646B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention provides a microorganism immobilized bio-based material and a preparation method and application thereof, belonging to the technical field of bio-based material preparation and liquor brewing. The composition comprises the following raw materials in parts by weight: 50-150 parts of water-retaining agent, 30-120 parts of potato starch, 50-150 parts of paper pulp fiber, 90-300 parts of water, 0.1-0.5 part of monascus purpureus, 0.1-0.5 part of aroma-producing yeast, 0.1-0.5 part of methane bacteria, 0.1-0.5 part of saccharomyces cerevisiae, 1000 parts of caproic acid bacteria 500 ion-doped materials, 20-40 parts of glucose, 10-20 parts of trehalose, 2-10 parts of retarder and 700 parts of cement 200 ion-doped materials. The microbial immobilized bio-based material prepared by the invention has the advantages of high caproic acid production efficiency, strong water retention capacity, no degradation of immobilized cells after multiple use, high thallus content and the like, and simultaneously, the raw materials are cheap and easy to obtain, and the preparation method is simple and easy to operate, so that the microbial immobilized bio-based material has good practical application value and wide industrial application prospect.
Description
Technical Field
The invention belongs to the technical field of preparation of bio-based materials and brewing of white spirit, and particularly relates to a microbial immobilized bio-based material and a preparation method and application thereof.
Background
The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
The Luzhou-flavor liquor is also called Luzhou-flavor liquor, and is the liquor with the largest yield, the largest variety and the widest coverage in China. The aroma components of the strong aromatic Chinese spirits are absolutely superior to ester components, and are the main body of the aroma components regardless of the quantity and the content, and account for about 60 percent of the total aroma components. Wherein the content of ethyl caproate is the crown of each trace component and is the highest content except ethanol and water. It has high absolute content, low threshold value and sweet and tasty taste. Therefore, the high content and low threshold of the ethyl caproate determine the main flavor characteristics of the aromatic white spirit.
Ethyl caproate is produced from caproic acid and ethanol by the action of esterases produced by monascus, usually by monascus, while the normal growth of caproic acid is not metabolized from methanobacteria and aroma-producing yeasts.
However, in the actual production process of the strong aromatic white spirit, the yield of ethyl caproate is generally insufficient, the standard of high-grade wine is difficult to achieve, and especially the ethyl caproate content of base wine produced in a newly-put cellar is low, so that ethyl caproate is often required to be supplemented in a later blending process, the enterprise cost is increased, time and labor are wasted, and the defect that the flavor of the whole wine is not coordinated due to the additional addition of ethyl caproate is caused.
Disclosure of Invention
Aiming at the defects of the prior art, the inventor provides a microorganism immobilized base material with high caproic acid production efficiency, strong water retention capacity, no degradation of immobilized cells after multiple use and high thallus content through long-term technical and practical exploration, and a preparation method and application thereof. The microbial immobilized base material prepared by the method can obviously improve the yield of ethyl acetate in the fermentation process of white spirit, and has high cyclic utilization rate and good industrial application prospect.
One of the objectives of the present invention is to provide a microorganism immobilized bio-based material.
The second objective of the present invention is to provide a method for preparing the above-mentioned microorganism-immobilized bio-based material.
The invention also aims to provide application of the microorganism immobilized bio-based material.
In order to achieve the purpose, the invention relates to the following technical scheme:
the invention provides a microorganism immobilized bio-based material, which consists of the following raw materials in parts by weight: 50-150 parts of water-retaining agent, 30-120 parts of potato starch, 50-150 parts of paper pulp fiber, 90-300 parts of water, 0.1-0.5 part of monascus purpureus, 0.1-0.5 part of aroma-producing yeast, 0.1-0.5 part of methane bacteria, 0.1-0.5 part of saccharomyces cerevisiae, 1000 parts of caproic acid bacteria 500 ion-doped materials, 20-40 parts of glucose, 10-20 parts of trehalose, 2-10 parts of retarder and 700 parts of cement 200 ion-doped materials;
wherein, the water-retaining agent is preferably peat;
the monascus, the aroma-producing yeast, the methane bacteria and the saccharomyces cerevisiae are all commercially available dry microbial agents;
the retarder is preferably a mixture of sodium gluconate and glucosamine, and the mass ratio of the sodium gluconate to the glucosamine is 1: 0.5 to 2;
further, the microorganism immobilized bio-based material is composed of the following raw materials in parts by weight: 50 parts of water-retaining agent, 30 parts of potato starch, 50 parts of paper pulp fiber, 90 parts of water, 0.1 part of monascus purpureus, 0.1 part of aroma-producing yeast, 0.2 part of methane bacteria, 0.1 part of saccharomyces cerevisiae, 500 parts of caproic acid bacteria, 20 parts of glucose, 10 parts of trehalose, 2 parts of retarder and 200 parts of cement.
Wherein the water-retaining agent is peat; the retarder is 1 part of sodium gluconate and 1 part of glucosamine;
further, the microorganism immobilized bio-based material is composed of the following raw materials in parts by weight: 150 parts of water-retaining agent, 120 parts of potato starch, 100 parts of paper pulp fiber, 250 parts of water, 0.5 part of monascus purpureus, 0.5 part of aroma-producing yeast, 0.1 part of methane bacteria, 0.5 part of saccharomyces cerevisiae, 800 parts of caproic acid bacteria, 40 parts of glucose, 15 parts of trehalose, 8 parts of retarder and 700 parts of cement;
wherein the water-retaining agent is peat; the retarder is 3 parts of sodium gluconate and 5 parts of glucosamine.
Further, the microorganism immobilized bio-based material is composed of the following raw materials in parts by weight: 100 parts of water-retaining agent, 60 parts of potato starch, 150 parts of paper pulp fiber, 300 parts of water, 0.2 part of monascus purpureus, 0.3 part of aroma-producing yeast, 0.5 part of methane bacteria, 0.3 part of saccharomyces cerevisiae, 1000 parts of caproic acid bacteria, 30 parts of glucose, 20 parts of trehalose, 8 parts of retarder and 700 parts of cement.
Wherein the water-retaining agent is peat; the retarder is 5 parts of sodium gluconate and 3 parts of glucosamine.
In a second aspect of the present invention, there is provided a method for preparing the above microorganism-immobilized bio-based material, the method comprising:
s1, adding water into the water-retaining agent, the potato starch, the paper pulp fiber, the glucose, the trehalose and the retarder according to the proportion and mixing;
s2, adding monascus, aroma-producing yeast, methane bacteria, saccharomyces cerevisiae, methane bacteria and caproic acid bacteria into the mixture obtained in the step S1, and stirring;
and S3, adding cement into the mixture after stirring in the step S2, and solidifying to obtain the microorganism immobilized bio-based material.
In the step S2, the stirring treatment temperature is controlled to be 28-32 ℃, and the stirring time is controlled to be 0.5-1 h;
in the step S3, the specific conditions of the solidification treatment are solidification at room temperature for 24-60 hours.
In a third aspect of the invention, the application of the microorganism immobilized bio-based material in the field of liquor brewing and/or the field of sewage treatment is provided.
Specifically, the microorganism immobilized bio-based material is laid around a white spirit brewing fermentation tank, so that the content of ethyl caproate in white spirit is increased, and the white spirit comprises but is not limited to strong aromatic white spirit;
or the like, or, alternatively,
the microorganism immobilized biological substrate is placed below a treatment tank in the sewage treatment process, so that the effect of filtering and purifying sewage is achieved.
The invention has the beneficial technical effects that:
the microorganism immobilized bio-based material prepared by the invention has the advantages ofThe microbial immobilized bio-based material prepared by the method has the advantages of high caproic acid production efficiency, strong water retention capacity, no degradation of the immobilized cells after multiple use, high thallus content and the like, and the maximum yield of caproic acid in unit time reaches 7.2g/L and is improved by about 2 times compared with the yield of non-immobilized caproic acid bacteria by experimental verification; the water retention capacity is excellent, and the maximum water content per unit mass is 51 percent; the yield of ethyl caproate in the fermentation process of the white spirit can reach 1.5-2.5 g/L; the batch stability is good, and the strain is not degenerated after being recycled for 22 times; the unit mass of the caproic acid bacteria can reach 1 multiplied by 106CFU/g。
Meanwhile, the raw materials of the microbial immobilized bio-based material are cheap and easy to obtain, and the preparation method is simple and easy to operate, so that the microbial immobilized bio-based material has good practical application value and wide industrial application prospect.
Drawings
FIG. 1 is an appearance diagram of the microorganism-immobilized bio-based material of example 2 of the present invention;
FIG. 2 is a graph showing the caproic acid production vs. free cell caproic acid production in example 2 of the present invention;
FIG. 3 is a graph showing the water content of the microorganism-immobilized bio-based material according to examples 1 to 3 of the present invention;
FIG. 4 is a graph showing the effect of recycling the microorganism-immobilized bio-based material of example 2 of the present invention.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
As mentioned above, in the actual production process of the existing Luzhou-flavor liquor, the yield of ethyl caproate is generally insufficient, the standard of high-grade liquor is difficult to achieve, and especially the ethyl caproate content of base liquor produced in a newly-put cellar is low, so that the ethyl caproate is frequently required to be supplemented in the later blending process, and the defects of time consumption, labor consumption and the like exist.
In view of the above, one embodiment of the present invention provides a microorganism immobilized bio-based material, which comprises the following raw materials in parts by weight: 50-150 parts of water-retaining agent, 30-120 parts of potato starch, 50-150 parts of paper pulp fiber, 90-300 parts of water, 0.1-0.5 part of monascus purpureus, 0.1-0.5 part of aroma-producing yeast, 0.1-0.5 part of methane bacteria, 0.1-0.5 part of saccharomyces cerevisiae, 1000 parts of caproic acid bacteria 500 ion-doped materials, 20-40 parts of glucose, 10-20 parts of trehalose, 2-10 parts of retarder and 700 parts of cement 200 ion-doped materials;
in yet another embodiment of the present invention, the water retaining agent is preferably peat;
in another embodiment of the present invention, the retarder is preferably a mixture of sodium gluconate and glucosamine, and the mass ratio of the sodium gluconate to the glucosamine is 1: 0.5 to 2;
in another embodiment of the present invention, the monascus, the aroma-producing yeast, the methane bacteria and the saccharomyces cerevisiae are all commercially available dry microbial agents;
the microorganism immobilized bio-based material disclosed by the invention mainly comprises the following raw materials:
peat: as a water retention agent, humus can be provided at the same time.
Potato starch: provides a carbon source for the growth and metabolism of microorganisms, and simultaneously, starch is consumed to form a cavity and increase gaps in the early foaming process.
Pulp fiber: the mechanical strength of the material is improved, the material is used as a material transportation channel in the later period, and meanwhile, the material has better water absorption.
Monascus: monascus can produce esterases and promote the synthesis of ethyl caproate.
Aroma-producing yeast: has good auxiliary function for the growth of caproic acid bacteria.
And (3) saccharomyces cerevisiae: gas can be generated in the metabolic process of the saccharomyces cerevisiae, and gaps in the material are increased.
Glucose: as a direct carbon source to provide energy to the microorganisms.
Trehalose: the trehalose can form a unique protective film on the cell surface under severe environmental conditions such as high temperature, high cold, high osmotic pressure, drying and dehydration, and the like, so that the protein molecules are effectively protected from invariance and inactivation, and the life process and biological characteristics of a living body are maintained.
Sodium gluconate: after a certain amount of sodium gluconate is added into the cement, the plasticity and strength of the concrete can be increased, and the initial and final setting periods of the concrete can be delayed.
Glucosamine: both glucosamine and sodium gluconate have the function of slowly coagulating cement.
Caproic acid bacteria: the main function is to produce caproic acid.
Methane bacteria: has good auxiliary function for the growth of caproic acid bacteria.
Cement: the biological matrix material is used as a main component of the biological matrix material and plays a role in fixing coagulation.
The microorganism immobilized bio-based material has higher mechanical strength and water retention, the cavity left after the potato starch is consumed is used as a reaction area of microorganisms such as caproic acid bacteria and the like, the trehalose can optimize the living environment of the microorganisms and prevent the microorganisms from losing water and dying in an extreme environment, and the pulp fibers are connected with different cavities to be used as channels for conveying nutrients and outputting caproic acid and ethyl caproate.
In another embodiment of the present invention, the microorganism immobilized bio-based material comprises the following raw materials by weight: 50 parts of water-retaining agent, 30 parts of potato starch, 50 parts of paper pulp fiber, 90 parts of water, 0.1 part of monascus purpureus, 0.1 part of aroma-producing yeast, 0.2 part of methane bacteria, 0.1 part of saccharomyces cerevisiae, 500 parts of caproic acid bacteria, 20 parts of glucose, 10 parts of trehalose, 2 parts of retarder and 200 parts of cement.
In yet another embodiment of the present invention, the water retaining agent is peat; the retarder is 1 part of sodium gluconate and 1 part of glucosamine;
in another embodiment of the present invention, the microorganism immobilized bio-based material comprises the following raw materials by weight: 150 parts of water-retaining agent, 120 parts of potato starch, 100 parts of paper pulp fiber, 250 parts of water, 0.5 part of monascus purpureus, 0.5 part of aroma-producing yeast, 0.1 part of methane bacteria, 0.5 part of saccharomyces cerevisiae, 800 parts of caproic acid bacteria, 40 parts of glucose, 15 parts of trehalose, 8 parts of retarder and 700 parts of cement;
in yet another embodiment of the present invention, the water retaining agent is peat; the retarder is 3 parts of sodium gluconate and 5 parts of glucosamine.
In another embodiment of the present invention, the microorganism immobilized bio-based material comprises the following raw materials by weight: 100 parts of water-retaining agent, 60 parts of potato starch, 150 parts of paper pulp fiber, 300 parts of water, 0.2 part of monascus purpureus, 0.3 part of aroma-producing yeast, 0.5 part of methane bacteria, 0.3 part of saccharomyces cerevisiae, 1000 parts of caproic acid bacteria, 30 parts of glucose, 20 parts of trehalose, 8 parts of retarder and 700 parts of cement.
In yet another embodiment of the present invention, the water retaining agent is peat; the retarder is 5 parts of sodium gluconate and 3 parts of glucosamine.
In another embodiment of the present invention, there is provided a method for preparing the above microorganism-immobilized bio-based material, the method comprising:
s1, adding water into the water-retaining agent, the potato starch, the paper pulp fiber, the glucose, the trehalose and the retarder according to the proportion and mixing;
s2, adding monascus, aroma-producing yeast, methane bacteria, saccharomyces cerevisiae, methane bacteria and caproic acid bacteria into the mixture obtained in the step S1, and stirring;
and S3, adding cement into the mixture after stirring in the step S2, and solidifying to obtain the microorganism immobilized bio-based material.
In another embodiment of the present invention, in step S2, the temperature of the stirring process is controlled to be 28 to 32 ℃, and the stirring time is controlled to be 0.5 to 1 hour;
in another embodiment of the present invention, in the step S3, the specific condition of the solidification process is solidification at room temperature for 24 to 60 hours.
In another embodiment of the present invention, the microorganism immobilized bio-based material is applied in the field of liquor brewing and/or sewage treatment.
In another embodiment of the invention, the microorganism immobilized bio-based material is laid around a white spirit brewing fermentation tank, so as to increase the content of ethyl hexanoate in white spirit, including but not limited to strong aromatic white spirit;
in another embodiment of the present invention, the microorganism-immobilized biological substrate is placed under a treatment tank during a sewage treatment process, so as to achieve the effect of filtering and purifying sewage.
The invention is further illustrated by the following examples, which are not to be construed as limiting the invention thereto. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The test methods in the following examples, which are not specified under specific conditions, are generally carried out under conventional conditions.
Example 1
In the embodiment of the invention, the microorganism immobilized bio-based material comprises the following components in parts by weight: 50 parts of water-retaining agent, 30 parts of potato starch, 50 parts of paper pulp fiber, 90 parts of water, 0.1 part of monascus purpureus, 0.1 part of aroma-producing yeast, 0.2 part of methane bacteria, 0.1 part of saccharomyces cerevisiae, 500 parts of caproic acid bacteria, 20 parts of glucose, 10 parts of trehalose, 2 parts of retarder and 200 parts of cement.
Wherein, the water-retaining agent is peat.
The retarder is 1 part of sodium gluconate and 1 part of glucosamine.
The raw materials are prepared by the following steps:
mixing water-retaining agent, potato starch, paper pulp fiber, glucose, trehalose, retarder and purified water according to a ratio, adding pure cultured monascus purpureus, aroma-producing yeast, methane bacteria, saccharomyces cerevisiae, methane bacteria and caproic acid bacteria according to a ratio, controlling the temperature at 28 ℃, continuously stirring for 30 minutes, adding cement according to a ratio, and solidifying for 24 hours at normal temperature.
Example 2
In the embodiment of the invention, the microorganism immobilized bio-based material comprises the following components in parts by weight: 150 parts of water-retaining agent, 120 parts of potato starch, 100 parts of paper pulp fiber, 250 parts of water, 0.5 part of monascus purpureus, 0.5 part of aroma-producing yeast, 0.1 part of methane bacteria, 0.5 part of saccharomyces cerevisiae, 800 parts of caproic acid bacteria, 40 parts of glucose, 15 parts of trehalose, 8 parts of retarder and 700 parts of cement.
Wherein, the water-retaining agent is peat.
The retarder is 3 parts of sodium gluconate and 5 parts of glucosamine.
The raw materials are prepared by the following steps:
mixing water-retaining agent, potato starch, paper pulp fiber, glucose, trehalose, retarder and purified water according to a ratio, adding pure cultured monascus purpureus, aroma-producing yeast, methane bacteria, saccharomyces cerevisiae, methane bacteria and caproic acid bacteria according to a ratio, controlling the temperature to be 30 ℃, continuously stirring for 40 minutes, adding cement according to a ratio, and solidifying for 60 hours at normal temperature.
Example 3
In the embodiment of the invention, the microorganism immobilized bio-based material comprises the following components in parts by weight: 100 parts of water-retaining agent, 60 parts of potato starch, 150 parts of paper pulp fiber, 300 parts of water, 0.2 part of monascus purpureus, 0.3 part of aroma-producing yeast, 0.5 part of methane bacteria, 0.3 part of saccharomyces cerevisiae, 1000 parts of caproic acid bacteria, 30 parts of glucose, 20 parts of trehalose, 8 parts of retarder and 700 parts of cement.
Wherein, the water-retaining agent is peat.
The retarder is 5 parts of sodium gluconate and 3 parts of glucosamine.
The raw materials are prepared by the following steps:
mixing water-retaining agent, potato starch, paper pulp fiber, glucose, trehalose, retarder and purified water according to a ratio, adding pure cultured monascus purpureus, aroma-producing yeast, methane bacteria, saccharomyces cerevisiae, methane bacteria and caproic acid bacteria according to a ratio, controlling the temperature to be 32 ℃, continuously stirring for 50 minutes, adding cement according to a ratio, and solidifying for 48 hours at normal temperature.
It should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the examples given, those skilled in the art can modify the technical solution of the present invention as needed or equivalent substitutions without departing from the spirit and scope of the technical solution of the present invention.
Claims (12)
1. The microbial immobilized bio-based material is characterized by comprising the following raw materials in parts by weight: 50-150 parts of water-retaining agent, 30-120 parts of potato starch, 50-150 parts of paper pulp fiber, 90-300 parts of water, 0.1-0.5 part of monascus purpureus, 0.1-0.5 part of aroma-producing yeast, 0.1-0.5 part of methane bacteria, 0.1-0.5 part of saccharomyces cerevisiae, 1000 parts of caproic acid bacteria 500 ion-doped materials, 20-40 parts of glucose, 10-20 parts of trehalose, 2-10 parts of retarder and 700 parts of cement 200 ion-doped materials.
2. The microbe-immobilized bio-based material of claim 1, wherein the water retaining agent is peat.
3. The microorganism-immobilized bio-based material of claim 1, wherein the retarder is a mixture of sodium gluconate and glucosamine, and the mass ratio of the sodium gluconate to the glucosamine is 1: 0.5 to 2.
4. The microorganism immobilized bio-based material of claim 1, which is prepared from the following raw materials in parts by weight: 50 parts of water-retaining agent, 30 parts of potato starch, 50 parts of paper pulp fiber, 90 parts of water, 0.1 part of monascus purpureus, 0.1 part of aroma-producing yeast, 0.2 part of methane bacteria, 0.1 part of saccharomyces cerevisiae, 500 parts of caproic acid bacteria, 20 parts of glucose, 10 parts of trehalose, 2 parts of retarder and 200 parts of cement;
wherein the water-retaining agent is peat; the retarder is 1 part of sodium gluconate and 1 part of glucosamine.
5. The microorganism immobilized bio-based material of claim 1, which is prepared from the following raw materials in parts by weight: 150 parts of water-retaining agent, 120 parts of potato starch, 100 parts of paper pulp fiber, 250 parts of water, 0.5 part of monascus purpureus, 0.5 part of aroma-producing yeast, 0.1 part of methane bacteria, 0.5 part of saccharomyces cerevisiae, 800 parts of caproic acid bacteria, 40 parts of glucose, 15 parts of trehalose, 8 parts of retarder and 700 parts of cement;
the water-retaining agent is peat; the retarder is 3 parts of sodium gluconate and 5 parts of glucosamine.
6. The microorganism immobilized bio-based material of claim 1, which is prepared from the following raw materials in parts by weight: 100 parts of water-retaining agent, 60 parts of potato starch, 150 parts of paper pulp fiber, 300 parts of water, 0.2 part of monascus purpureus, 0.3 part of aroma-producing yeast, 0.5 part of methane bacteria, 0.3 part of saccharomyces cerevisiae, 1000 parts of caproic acid bacteria, 30 parts of glucose, 20 parts of trehalose, 8 parts of retarder and 700 parts of cement;
the water-retaining agent is peat; the retarder is 5 parts of sodium gluconate and 3 parts of glucosamine.
7. A process for the preparation of a microbial immobilized biobased material according to any one of claims 1 to 6, wherein the process comprises:
s1, adding water into the water-retaining agent, the potato starch, the paper pulp fiber, the glucose, the trehalose and the retarder according to the proportion and mixing;
s2, adding monascus, aroma-producing yeast, methane bacteria, saccharomyces cerevisiae, methane bacteria and caproic acid bacteria into the mixture obtained in the step S1, and stirring;
and S3, adding cement into the mixture after stirring in the step S2, and solidifying to obtain the microorganism immobilized bio-based material.
8. The method according to claim 7, wherein in step S2, the temperature of the stirring is controlled to be 28-32 ℃ and the stirring time is controlled to be 0.5-1 h.
9. The method according to claim 7, wherein in step S3, the specific conditions of the solidification treatment are 24-60 hours at room temperature.
10. Use of the microbial immobilized bio-based material according to any one of claims 1 to 6 and/or the microbial immobilized bio-based material prepared by the preparation method according to any one of claims 7 to 9 in the field of liquor brewing.
11. The microorganism-immobilized bio-based material according to claim 10, which is applied in the field of liquor brewing by building the microorganism-immobilized bio-based material around a liquor brewing fermentation tank.
12. The use according to claim 11, wherein the white spirit is Luzhou-flavor white spirit.
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| CN102229874B (en) * | 2011-06-24 | 2012-07-18 | 泸州品创科技有限公司 | Production method of Luzhou-flavor liquor |
| CN103497896B (en) * | 2013-10-16 | 2016-03-09 | 济南瑞丰生物工程有限公司 | A kind of dehydration pit mud functional bacteria protective material and application thereof |
| CN105349400B (en) * | 2015-12-14 | 2018-05-25 | 武汉雅仕博科技有限公司 | A kind of method of cave type pit, pit mud and its brewing aroma type base liquor |
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2018
- 2018-11-19 CN CN201811378868.XA patent/CN109439646B/en active Active
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