CN109439646A - A kind of microbial immobilized bio-based materials and its preparation method and application - Google Patents
A kind of microbial immobilized bio-based materials and its preparation method and application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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Abstract
The present invention provides a kind of microbial immobilized bio-based materials and its preparation method and application, belongs to bio-based materials preparation and Liquor Making Technology field.It is made of the raw material of following weight: 50-150 parts of water-retaining agent, 30-120 parts of potato starch, 50-150 parts of paper pulp fiber, 90-300 parts of water, 0.1-0.5 parts of monascus ruber, 0.1-0.5 parts of aroma-producing yeasts bacterium, 0.1-0.5 parts of methane backeria, 0.1-0.5 parts of S. cervisiae, 500-1000 parts of caproic acid bacteria, 20-40 parts of glucose, 10-20 parts of trehalose, 2-10 parts of retarder, 200-700 parts of cement.The microbial immobilized bio-based materials that the present invention is prepared have many advantages, such as to produce caproic acid is high-efficient, water holding capacity is strong, immobilized cell be used for multiple times do not degenerate, thallus content it is high, raw material is cheap and easy to get simultaneously, preparation method is simple, it is easy to operate, therefore value and wide industrial applications prospect with good practical application.
Description
Technical field
The invention belongs to bio-based materials preparation and Liquor Making Technology fields, and in particular to a kind of microbial immobilized life
Object sill and its preparation method and application.
Background technique
Disclosing the information of the background technology part, it is only intended to increase understanding of the overall background of the invention, without certainty
It is considered as recognizing or implying in any form that information composition has become existing skill well known to persons skilled in the art
Art.
Luzhou-flavor liquo is also known as Lu aromatic white spirit, it is that yield is maximum in China's white wine, and kind is most, and covering surface is most wide
A kind of white wine.The flavor component of Luzhou-flavor liquo, is had comparative advantage with lactone component, no matter quantitatively or in content all
It ranks first, it is the main body of this odor type ingredient, accounts about the 60% of total flavor component content.Wherein the content of ethyl hexanoate is again
It is the hat of each micro constitutent, is the highest ingredient of content in addition to ethanol and water.Not only absolute content is high for it, and threshold value compared with
It is low, also with sweet taste, tasty and refreshing in the sense of taste.Therefore, the high-content of ethyl hexanoate, Low threshold determine the master of this kind of aromatic white spirit
Want flavor characteristic.
Ethyl hexanoate is generated under the action of Esterified Enzyme by caproic acid and ethyl alcohol, and caproic acid is originated from caproic acid bacteria, and Esterified Enzyme is logical
Often generated by monascus ruber, and the normal growth of caproic acid bacteria is metabolized and be unable to do without methane backeria and aroma-producing yeasts.
But in Luzhou-flavor liquo actual production process, generally existing ethyl hexanoate underproduces, it is difficult to reach top grade wine
Standard, its ethyl hexanoate content of the base liquor of especially new operation pit life is lower, therefore generally requires later period allocation process and add
Ethyl hexanoate not only increases entreprise cost, time-consuming and laborious, while additional addition ethyl hexanoate may cause the whole wine body wind of generation
The defects of taste is uncoordinated.
Summary of the invention
In view of the above shortcomings of the prior art, inventor provides a kind of production caproic acid effect through long-term technology and practical exploration
Rate is high, water holding capacity is strong, immobilized cell be used for multiple times do not degenerate, the microbial immobilized sill that thallus content is high and its system
Preparation Method and application.The microbial immobilized sill being prepared using the application, can significantly improve liquor fermentation process
Middle ethyl acetate yield, while cyclic utilization rate is high, has good industrial applications prospect.
One of the objects of the present invention is to provide a kind of microbial immobilized bio-based materials.
The second object of the present invention is to provide the preparation method of mentioned microorganism immobilization biological sill.
The third object of the present invention is to provide the application of mentioned microorganism immobilization biological sill.
To achieve the above object, the present invention relates to following technical schemes:
The first aspect of the invention provides a kind of microbial immobilized bio-based materials, by following weight
Raw material composition: 50-150 parts of water-retaining agent, 30-120 parts of potato starch, 50-150 parts of paper pulp fiber, 90-300 parts of water, monascus
0.1-0.5 parts of bacterium, 0.1-0.5 parts of aroma-producing yeasts bacterium, 0.1-0.5 parts of methane backeria, 0.1-0.5 parts of S. cervisiae, caproic acid bacteria
500-1000 parts, 20-40 parts of glucose, 10-20 parts of trehalose, 2-10 parts of retarder, 200-700 parts of cement；
Wherein, the water-retaining agent is preferably peat；
The monascus ruber, aroma-producing yeasts bacterium, methane backeria, S. cervisiae are commercially available dry microbial inoculum；
The retarder is preferably the mixture of sodium gluconate and Glucosamine, and the two mass ratio is 1:0.5~2；
Further, the microbial immobilized bio-based materials, are made of the raw material of following weight: water conservation
50 parts of agent, 30 parts of potato starch, 50 parts of paper pulp fiber, 90 parts of water, 0.1 part of monascus ruber, 0.1 part of aroma-producing yeasts bacterium, methane backeria
0.2 part, 0.1 part of S. cervisiae, 500 parts of caproic acid bacteria, 20 parts of glucose, 10 parts of trehalose, 2 parts of retarder, 200 parts of cement.
Wherein, the water-retaining agent is peat；The retarder is 1 part of sodium gluconate, 1 part of Glucosamine；
Further, the microbial immobilized bio-based materials, are made of the raw material of following weight: water conservation
150 parts of agent, 120 parts of potato starch, 100 parts of paper pulp fiber, 250 parts of water, 0.5 part of monascus ruber, 0.5 part of aroma-producing yeasts bacterium, first
0.1 part of alkane bacterium, 0.5 part of S. cervisiae, 800 parts of caproic acid bacteria, 40 parts of glucose, 15 parts of trehalose, 8 parts of retarder, cement 700
Part；
Wherein, the water-retaining agent is peat；The retarder is 3 parts of sodium gluconate, 5 parts of Glucosamine.
Further, the microbial immobilized bio-based materials, are made of the raw material of following weight: water conservation
100 parts of agent, 60 parts of potato starch, 150 parts of paper pulp fiber, 300 parts of water, 0.2 part of monascus ruber, 0.3 part of aroma-producing yeasts bacterium, methane
0.5 part of bacterium, 0.3 part of S. cervisiae, 1000 parts of caproic acid bacteria, 30 parts of glucose, 20 parts of trehalose, 8 parts of retarder, cement 700
Part.
Wherein, the water-retaining agent is peat；The retarder is 5 parts of sodium gluconate, 3 parts of Glucosamine.
The second aspect of the invention provides the preparation method of mentioned microorganism immobilization biological sill, the preparation
Method includes:
S1, water is added to mix according to the ratio water-retaining agent, potato starch, paper pulp fiber, glucose, trehalose and retarder；
S2, monascus ruber, aroma-producing yeasts bacterium, methane backeria, S. cervisiae, methane backeria and caproic acid bacteria are added step S1's
It is stirred in mixture；
S3, by cement be added step S2 in stir process after mixture in, solidify to obtain microbial immobilized biology base
Material.
Wherein, in the step S2, for stir process controlled at 28~32 DEG C, mixing time control is 0.5~1h；
In the step S3, solidification processing actual conditions are to solidify 24~60 hours under room temperature.
The third aspect of the invention, provide mentioned microorganism immobilization biological sill in brewed spirit field and/or
Application in sewage treatment field.
Specifically, the application mode is to build mentioned microorganism immobilization biological sill in brewed spirit fermentation vat
Around, to improve ethyl hexanoate content in white wine, the white wine includes but is not limited to Luzhou-flavor liquo；
Or,
Mentioned microorganism immobilization biological substrate is placed in below processing pond in sewage disposal process, to reach filtering
The effect of purification of sewage water.
Advantageous effects of the invention:
Caproic acid is high-efficient, water holding capacity is strong, solid with producing for the microbial immobilized bio-based materials that the present invention is prepared
Surely change cell be used for multiple times do not degenerate, the advantages that thallus content is high, experiment proves that, fixed using microorganism prepared by the present invention
Change bio-based materials, caproic acid maximum production reaches 7.2g/L in the unit time, compares on-fixed caproic acid bacteria output increased about 2
Times；Water holding capacity is excellent, and unit mass maximum water holding capacity is 51%；Ethyl hexanoate yield is up to 1.5- during liquor fermentation
2.5g/L；Lot stability is good, and 22 bacterial strains are recycled and do not degenerate；Unit mass caproic acid bacteria biomass is up to 1 × 106CFU/
g。
Meanwhile the microbial immobilized bio-based materials raw material of the present invention is cheap and easy to get, and preparation method is simple, and it is easy to operate, because
This value and wide industrial applications prospect with good practical application.
Detailed description of the invention
Fig. 1 is that the microbial immobilized bio-based materials of the embodiment of the present invention 2 are apparently schemed；
Fig. 2 is the caproic acid yield and free cell caproic acid yield comparative diagram of the embodiment of the present invention 2；
Fig. 3 is the aqueous spirogram of microbial immobilized bio-based materials of 1-3 of the embodiment of the present invention；
Fig. 4 is that batch effect picture is recycled in the microbial immobilized bio-based materials of the embodiment of the present invention 2.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation, device, component and/or their combination.
As previously mentioned, generally existing ethyl hexanoate underproduces in existing Luzhou-flavor liquo actual production process, it is difficult to
Reach top grade wine standard, its ethyl hexanoate content of the base liquor of especially new operation pit life is lower, therefore generally requires later period tune
Ethyl hexanoate is added with process, there is the defects of taking time and effort.
In view of this, a kind of microbial immobilized bio-based materials are provided in the specific embodiment of the present invention,
Be made of the raw material of following weight: 50-150 parts of water-retaining agent, 30-120 parts of potato starch, 50-150 parts of paper pulp fiber,
90-300 parts of water, 0.1-0.5 parts of monascus ruber, 0.1-0.5 parts of aroma-producing yeasts bacterium, 0.1-0.5 parts of methane backeria, S. cervisiae
0.1-0.5 parts, 500-1000 parts of caproic acid bacteria, 20-40 parts of glucose, 10-20 parts of trehalose, 2-10 parts of retarder, cement 200-
700 parts；
In still another embodiment of the invention, the water-retaining agent is preferably peat；
In still another embodiment of the invention, the retarder is preferably the mixed of sodium gluconate and Glucosamine
Object is closed, the two mass ratio is 1:0.5~2；
In still another embodiment of the invention, the monascus ruber, aroma-producing yeasts bacterium, methane backeria, S. cervisiae
It is commercially available dry microbial inoculum；
The microbial immobilized each raw material main function of bio-based materials of the present invention is as follows:
Peat: as water-retaining agent, while humus can be provided.
Potato starch: carbon source is provided for microorganism growth metabolism, while in foaming process early period, consumption of starch forms sky
Chamber increases gap.
Paper pulp fiber: improving the mechanical strength of material, and the later period is as matter transportation channel, while it is preferable to have material
Water imbibition.
Monascus: monascus can generate Esterified Enzyme, promote the synthesis of ethyl hexanoate.
Aroma-producing yeasts: there is good miscellaneous function to the growth of caproic acid bacteria.
Saccharomyces cerevisiae: can produce gas in saccharomyces cerevisiae metabolic process, increase the gap in material.
Glucose: energy is provided for microorganism as direct carbon source.
Trehalose: trehalose is under the severe environmental conditions such as high temperature, high and cold, hyperosmosis and dry dehydration in cell surface
Unique protective film can be formed, be effectively protected protein molecule invariance inactivation, thus the life process for the body that sustains life and
Biological characteristic.
Sodium gluconate: after adding certain amount sodium gluconate in cement, can increase the plasticity and intensity of concrete,
And there is the initial and final solidification period for postponing concrete.
Glucosamine: Glucosamine and sodium gluconate all have the function of making cement slow coagulation.
Caproic acid bacteria: main function is to produce caproic acid.
Methane backeria: there is good miscellaneous function to the growth of caproic acid bacteria.
Cement: as the bulk composition of bio-based materials, play the role of fixed condensation.
Mentioned microorganism immobilization biological sill mechanical strength with higher and water-retaining property, after potato starch is consumed
Conversion zone of the cavity left as microorganisms such as caproic acid bacterias, trehalose can optimize microbe survival environment, prevent micro- life
Object dehydration in extreme environment is dead, and paper pulp fiber connects different cavitys as conveying nutrient and output caproic acid and ethyl hexanoate
Channel.
In still another embodiment of the invention, the microbial immobilized bio-based materials, by following parts by weight
The raw material of proportion forms: 50 parts of water-retaining agent, 30 parts of potato starch, 50 parts of paper pulp fiber, 90 parts of water, 0.1 part of monascus ruber, raw perfume
0.1 part of saccharomycete, 0.1 part of S. cervisiae, 500 parts of caproic acid bacteria, 20 parts of glucose, 10 parts of trehalose, delays 0.2 part of methane backeria
Solidifying 2 parts of agent, 200 parts of cement.
In still another embodiment of the invention, the water-retaining agent is peat；The retarder is sodium gluconate 1
Part, 1 part of Glucosamine；
In still another embodiment of the invention, the microbial immobilized bio-based materials, by following parts by weight
The raw material of proportion forms: 150 parts of water-retaining agent, 120 parts of potato starch, 100 parts of paper pulp fiber, 250 parts of water, 0.5 part of monascus ruber,
0.5 part of aroma-producing yeasts bacterium, 0.1 part of methane backeria, 0.5 part of S. cervisiae, 800 parts of caproic acid bacteria, 40 parts of glucose, trehalose 15
Part, 8 parts of retarder, 700 parts of cement；
In still another embodiment of the invention, the water-retaining agent is peat；The retarder is sodium gluconate 3
Part, 5 parts of Glucosamine.
In still another embodiment of the invention, the microbial immobilized bio-based materials, by following parts by weight
The raw material of proportion forms: 100 parts of water-retaining agent, 60 parts of potato starch, 150 parts of paper pulp fiber, 300 parts of water, 0.2 part of monascus ruber,
0.3 part of aroma-producing yeasts bacterium, 0.5 part of methane backeria, 0.3 part of S. cervisiae, 1000 parts of caproic acid bacteria, 30 parts of glucose, trehalose 20
Part, 8 parts of retarder, 700 parts of cement.
In still another embodiment of the invention, the water-retaining agent is peat；The retarder is sodium gluconate 5
Part, 3 parts of Glucosamine.
In still another embodiment of the invention, the preparation method of mentioned microorganism immobilization biological sill is provided,
The preparation method includes:
S1, water is added to mix according to the ratio water-retaining agent, potato starch, paper pulp fiber, glucose, trehalose and retarder；
S2, monascus ruber, aroma-producing yeasts bacterium, methane backeria, S. cervisiae, methane backeria and caproic acid bacteria are added step S1's
It is stirred in mixture；
S3, by cement be added step S2 in stir process after mixture in, solidify to obtain microbial immobilized biology base
Material.
In still another embodiment of the invention, in the step S2, stir process is stirred controlled at 28~32 DEG C
Mixing time control is 0.5~1h；
In still another embodiment of the invention, in the step S3, solidification processing actual conditions are to solidify under room temperature
24~60 hours.
In still another embodiment of the invention, mentioned microorganism immobilization biological sill is provided and is led in brewed spirit
Application in domain and/or sewage treatment field.
In still another embodiment of the invention, the application mode is by mentioned microorganism immobilization biological substrate
Material is built around brewed spirit fermentation vat, to improve ethyl hexanoate content in white wine, the white wine includes but is not limited to giving off a strong fragrance
Type white wine；
In still another embodiment of the invention, the application mode is that mentioned microorganism immobilization biological substrate exists
It is placed in sewage disposal process below processing pond, to achieve the effect that Purification by filtration sewage.
Explanation is further explained to the present invention by the following examples, but is not construed as limiting the invention.It should be understood that
These examples are only for illustrating the present invention and are not intended to limit the scope of the present invention.Actual conditions are not specified in the following example
Test method, carry out usually according to normal condition.
Embodiment 1
In the embodiment of the present invention, microbial immobilized bio-based materials component by weight ratio is as follows: 50 parts of water-retaining agent,
30 parts of potato starch, 90 parts of water, 0.1 part of monascus ruber, 0.1 part of aroma-producing yeasts bacterium, 0.2 part of methane backeria, is made 50 parts of paper pulp fiber
0.1 part of brewer yeast bacterium, 500 parts of caproic acid bacteria, 20 parts of glucose, 10 parts of trehalose, 2 parts of retarder, 200 parts of cement.
Wherein, the water-retaining agent is peat.
The retarder is 1 part of sodium gluconate, 1 part of Glucosamine.
Above-mentioned raw materials are made by the steps:
Pure water is added to mix according to the ratio water-retaining agent, potato starch, paper pulp fiber, glucose, trehalose, retarder, it will
Monascus ruber, aroma-producing yeasts bacterium, methane backeria, S. cervisiae, methane backeria, the caproic acid bacteria of purebred culture are added according to the ratio, control
It 28 DEG C of temperature, persistently stirs 30 minutes, is added cement according to the ratio, air-setting 24 hours.
Embodiment 2
In the embodiment of the present invention, microbial immobilized bio-based materials component by weight ratio is as follows: water-retaining agent 150
Part, 120 parts of potato starch, 100 parts of paper pulp fiber, 250 parts of water, 0.5 part of monascus ruber, 0.5 part of aroma-producing yeasts bacterium, methane backeria
0.1 part, 0.5 part of S. cervisiae, 800 parts of caproic acid bacteria, 40 parts of glucose, 15 parts of trehalose, 8 parts of retarder, 700 parts of cement.
Wherein, the water-retaining agent is peat.
The retarder is 3 parts of sodium gluconate, 5 parts of Glucosamine.
Above-mentioned raw materials are made by the steps:
Pure water is added to mix according to the ratio water-retaining agent, potato starch, paper pulp fiber, glucose, trehalose, retarder, it will
Monascus ruber, aroma-producing yeasts bacterium, methane backeria, S. cervisiae, methane backeria, the caproic acid bacteria of purebred culture are added according to the ratio, control
It 30 DEG C of temperature, persistently stirs 40 minutes, is added cement according to the ratio, air-setting 60 hours.
Embodiment 3
In the embodiment of the present invention, microbial immobilized bio-based materials component by weight ratio is as follows: water-retaining agent 100
Part, 60 parts of potato starch, 150 parts of paper pulp fiber, 300 parts of water, 0.2 part of monascus ruber, 0.3 part of aroma-producing yeasts bacterium, methane backeria 0.5
Part, 0.3 part of S. cervisiae, 1000 parts of caproic acid bacteria, 30 parts of glucose, 20 parts of trehalose, 8 parts of retarder, 700 parts of cement.
Wherein, the water-retaining agent is peat.
The retarder is 5 parts of sodium gluconate, 3 parts of Glucosamine.
Above-mentioned raw materials are made by the steps:
Pure water is added to mix according to the ratio water-retaining agent, potato starch, paper pulp fiber, glucose, trehalose, retarder, it will
Monascus ruber, aroma-producing yeasts bacterium, methane backeria, S. cervisiae, methane backeria, the caproic acid bacteria of purebred culture are added according to the ratio, control
It 32 DEG C of temperature, persistently stirs 50 minutes, is added cement according to the ratio, air-setting 48 hours.
It should be noted that above example is only used to illustrate the technical scheme of the present invention rather than is limited.Although ginseng
It is described the invention in detail according to given example, but those skilled in the art can be as needed to this hair
Bright technical solution is modified or replaced equivalently, without departing from the spirit and scope of the technical solution of the present invention.
Claims (10)
1. a kind of microbial immobilized bio-based materials, which is characterized in that be made of the raw material of following weight: water-retaining agent
50-150 parts, 30-120 parts of potato starch, 50-150 parts of paper pulp fiber, 90-300 parts of water, 0.1-0.5 parts of monascus ruber, raw perfume
0.1-0.5 parts of saccharomycete, 0.1-0.5 parts of methane backeria, 0.1-0.5 parts of S. cervisiae, 500-1000 parts of caproic acid bacteria, glucose
20-40 parts, 10-20 parts of trehalose, 2-10 parts of retarder, 200-700 parts of cement.
2. a kind of microbial immobilized bio-based materials as described in claim 1, which is characterized in that the water-retaining agent is mud
Charcoal.
3. a kind of microbial immobilized bio-based materials as described in claim 1, which is characterized in that the retarder is grape
The mass ratio of the mixture of sodium saccharate and Glucosamine, sodium gluconate and Glucosamine is 1:0.5~2.
4. a kind of microbial immobilized bio-based materials as described in claim 1, which is characterized in that described microbial immobilized
Bio-based materials are made of the raw material of following weight: 50 parts of water-retaining agent, 30 parts of potato starch, 50 parts of paper pulp fiber,
90 parts of water, 0.1 part of monascus ruber, 0.1 part of aroma-producing yeasts bacterium, 0.2 part of methane backeria, 0.1 part of S. cervisiae, 500 parts of caproic acid bacteria,
20 parts of glucose, 10 parts of trehalose, 2 parts of retarder, 200 parts of cement；
Wherein, the water-retaining agent is peat；The retarder is 1 part of sodium gluconate, 1 part of Glucosamine.
5. a kind of microbial immobilized bio-based materials as described in claim 1, which is characterized in that described microbial immobilized
Bio-based materials are made of the raw material of following weight: 150 parts of water-retaining agent, 120 parts of potato starch, paper pulp fiber 100
Part, 250 parts of water, 0.5 part of monascus ruber, 0.5 part of aroma-producing yeasts bacterium, 0.1 part of methane backeria, 0.5 part of S. cervisiae, caproic acid bacteria
800 parts, 40 parts of glucose, 15 parts of trehalose, 8 parts of retarder, 700 parts of cement；
The water-retaining agent is peat；The retarder is 3 parts of sodium gluconate, 5 parts of Glucosamine.
6. a kind of microbial immobilized bio-based materials as described in claim 1, which is characterized in that described microbial immobilized
Bio-based materials are made of the raw material of following weight: 100 parts of water-retaining agent, 60 parts of potato starch, paper pulp fiber 150
Part, 300 parts of water, 0.2 part of monascus ruber, 0.3 part of aroma-producing yeasts bacterium, 0.5 part of methane backeria, 0.3 part of S. cervisiae, caproic acid bacteria
1000 parts, 30 parts of glucose, 20 parts of trehalose, 8 parts of retarder, 700 parts of cement；
The water-retaining agent is peat；The retarder is 5 parts of sodium gluconate, 3 parts of Glucosamine.
7. the preparation method of any one of the claim 1-6 microbial immobilized bio-based materials, which is characterized in that the system
Preparation Method includes:
S1, water is added to mix according to the ratio water-retaining agent, potato starch, paper pulp fiber, glucose, trehalose and retarder；
S2, the mixing that monascus ruber, aroma-producing yeasts bacterium, methane backeria, S. cervisiae, methane backeria and caproic acid bacteria are added to step S1
It is stirred in object；
S3, by cement be added step S2 in stir process after mixture in, solidify to obtain microbial immobilized bio-based materials.
8. a kind of preparation method as claimed in claim 7, which is characterized in that in the step S2, stir process controls temperature
It is 28~32 DEG C, mixing time control is 0.5~1h.
9. a kind of preparation method as claimed in claim 7, which is characterized in that in the step S3, solidification processing actual conditions
To be solidified 24~60 hours under room temperature.
10. described in any one of the claim 1-7 microbial immobilized bio-based materials and/or claim any one of 7-9
Microbial immobilized bio-based materials the answering in brewed spirit field and/or sewage treatment field that preparation method is prepared
With；
Preferably,
The application mode is to build mentioned microorganism immobilization biological sill around brewed spirit fermentation vat, described white
Wine includes but is not limited to Luzhou-flavor liquo；
Or,
The application mode is to be placed in mentioned microorganism immobilization biological substrate below processing pond in sewage disposal process.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115536343A (en) * | 2022-10-28 | 2022-12-30 | 中建西部建设贵州有限公司 | Slump-retaining self-compacting high-strength concrete for high-temperature areas and preparation method thereof |
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| CN102229874A (en) * | 2011-06-24 | 2011-11-02 | 泸州品创科技有限公司 | Production method of Luzhou-flavor liquor |
| CN103497896A (en) * | 2013-10-16 | 2014-01-08 | 济南瑞丰生物工程有限公司 | Dried pit mud functional bacteria protectant and application thereof |
| CN105349400A (en) * | 2015-12-14 | 2016-02-24 | 武汉天龙黄鹤楼酒业有限公司 | Cave type pit, pit mud and method of brewing highly flavored type basic liquor by cave type pit and pit mud |
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| CN102229874A (en) * | 2011-06-24 | 2011-11-02 | 泸州品创科技有限公司 | Production method of Luzhou-flavor liquor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115536343A (en) * | 2022-10-28 | 2022-12-30 | 中建西部建设贵州有限公司 | Slump-retaining self-compacting high-strength concrete for high-temperature areas and preparation method thereof |
| CN115536343B (en) * | 2022-10-28 | 2023-10-20 | 中建西部建设贵州有限公司 | Slump-retaining self-compaction high-strength concrete for high-temperature areas and preparation method thereof |
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| CN109439646B (en) | 2021-02-05 |
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