CN109438773A - A method of using food galactan Zhuo Beiershi Flavobacterium degradation sodium alginate and agar composite membrane - Google Patents
A method of using food galactan Zhuo Beiershi Flavobacterium degradation sodium alginate and agar composite membrane Download PDFInfo
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- CN109438773A CN109438773A CN201811329992.7A CN201811329992A CN109438773A CN 109438773 A CN109438773 A CN 109438773A CN 201811329992 A CN201811329992 A CN 201811329992A CN 109438773 A CN109438773 A CN 109438773A
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- beiershi
- zhuo
- galactan
- flavobacterium
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- 230000015556 catabolic process Effects 0.000 title claims abstract description 32
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 32
- 241000589565 Flavobacterium Species 0.000 title claims abstract description 27
- 239000012528 membrane Substances 0.000 title claims abstract description 25
- 229920001817 Agar Polymers 0.000 title claims abstract description 23
- 239000008272 agar Substances 0.000 title claims abstract description 23
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000000661 sodium alginate Substances 0.000 title claims abstract description 21
- 235000010413 sodium alginate Nutrition 0.000 title claims abstract description 21
- 229940005550 sodium alginate Drugs 0.000 title claims abstract description 21
- 239000002131 composite material Substances 0.000 title claims abstract description 15
- 239000000243 solution Substances 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 238000002835 absorbance Methods 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 238000000643 oven drying Methods 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
- 239000005955 Ferric phosphate Substances 0.000 claims description 2
- 239000011575 calcium Substances 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 238000004132 cross linking Methods 0.000 claims description 2
- 230000000593 degrading effect Effects 0.000 claims description 2
- 229940032958 ferric phosphate Drugs 0.000 claims description 2
- WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical compound [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 claims description 2
- 229910000399 iron(III) phosphate Inorganic materials 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 238000005660 chlorination reaction Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 238000003556 assay Methods 0.000 abstract description 5
- 230000003413 degradative effect Effects 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 229940066779 peptones Drugs 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000893379 Zobellia galactanivorans Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/04—Alginic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/12—Agar-agar; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/04—Alginic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/12—Agar-agar; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/16—Halogen-containing compounds
- C08K2003/162—Calcium, strontium or barium halides, e.g. calcium, strontium or barium chloride
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Manufacturing & Machinery (AREA)
- Materials Engineering (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The present invention provides a kind of methods using food galactan Zhuo Beiershi Flavobacterium degradation sodium alginate and agar composite membrane, the sodium alginate prepared in advance and agar composite membrane disk are put into the culture solution containing food galactan Zhuo Beiershi Flavobacterium and degraded, the film being degraded is taken out after a period of time, then it is weighed after being filtered, washed, being freeze-dried using assay balance, it is compared with the film before degradation, the result shows that the method has relatively good degradation effect.The method of the present invention is safe and environment-friendly, convenient, can effectively avoid conventional chemical methods degradative membrane bring environmental pollution, has very wide application prospect.
Description
Technical field
The invention belongs to membrane degradation technical fields, and in particular to a kind of to be degraded using food galactan Zhuo Beiershi Flavobacterium
The method of sodium alginate and agar composite membrane.
Background technique
Eating galactan Zhuo Beiershi Flavobacterium (Zobellia galactanovorans) is a kind of Marine Bacteria, in nature
Existence range is wider in boundary.Bacterium growth is fast, does not allow easy in inactivation, thus with high application prospect.And wherein also containing spy
The effect of different degradation agar, more adds commercial application potentiality.
In the factors for causing environmental pollution, public hazards caused by plastic refuse have caused the extensive pass of society
Note.At the beginning of 21 century, the plastic package material dosage in China is very big, and annual consumption is up to 50,000,000 tons, 30% is if wherein had
Disposable foamed plastic, then national annual waste plastic will have 15,000,000 tons or more；It can be seen that research and development and production biology
Degradative membrane has become very urgent to alleviate environmental pollution.
Biodegradable films generally by the microbial action in soil and water or by the effect of the ultraviolet light in sunlight,
In the natural environment division degradation, although can degrade, degradation speed is more slow, and in air in general bacterium simultaneously
It will not be degraded, the membrane degradation technology of this raising degradation rate for just making exploitation new becomes one kind, and there is an urgent need to for this
A problem, the present invention develop a kind of using food galactan Zhuo Beiershi Flavobacterium degradation sodium alginate and agar composite membrane
Method, this method safety and environmental protection is pollution-free, and accelerates degradation rate.
Summary of the invention
It is a kind of using food galactan Zhuo Beiershi Flavobacterium degradation sodium alginate the purpose of the present invention is to provide providing
With the method for agar composite membrane, this method safety and environmental protection is pollution-free, and accelerates degradation rate.
To achieve the above object, the present invention adopts the following technical scheme:
A method of using food galactan Zhuo Beiershi Flavobacterium degradation sodium alginate and agar composite membrane comprising following
Step:
1) will eat galactan Zhuo Beiershi Flavobacterium is to cultivate 2-3 days in 35 DEG C of culture solution in temperature, then by its absorbance
It is adjusted to 0.3, obtains food galactan Zhuo Beiershi Flavobacterium culture solution, it is spare；
2) by 75mL mass fraction be 1% sodium alginate soln and 75mL mass fraction be 1% agar solution mix, 55
Pouring into diameter at DEG C after mixing evenly is to be then placed in baking oven in 190mm culture dish and dry 12h taking-up at 50 DEG C, add and contain
The mixed solution of 0.5 wt.% calcium chloride and 20vt.% dehydrated alcohol, solution submerge film, are put into baking oven drying system after being crosslinked 30min
It is spare at sequin；
3) the resulting sequin of step 2 is put into obtained by step 1) and is obtained in food galactan Zhuo Beiershi Flavobacterium culture solution
Degradation, takes out after placing 5 days in shaking table at 35 DEG C, film is come out with 200 mesh screens, is washed with distilled water 3-5 times, through cold
Lyophilizer is freeze-dried into dry film to get the sodium alginate and agar composite membrane to after degrading.
The raw material composition of culture solution described in step 1) is by weight are as follows: 5 parts of peptone, 1 part of yeast extract, high ferric phosphate
0.1 part, 30 parts of NaCl, 1000 parts of distilled water.
Remarkable advantage of the invention is:
(1) growth of food galactan Zhuo Beiershi Flavobacterium bacterium is fast, does not allow easy in inactivation, has excellent characteristic.Benefit of the invention
There is the effect for making agar membrane degradation with food galactan Zhuo Beiershi Flavobacterium, by sodium alginate and the compound membrane degradation of agar, and
Without can effectively avoid ring brought by conventional chemical methods degradative membrane using the reagent that may cause pollution to environment during it
The problem of border is polluted, is a kind of environment amenable membrane degradation method.
(2) process film degradation time is shorter in the present invention, and method is convenient, quickly, no pollution to the environment.
(3) reaction condition of the present invention is mild, is not necessarily to high temperature and pressure, does not need special reactor.
Specific embodiment
In order to make content of the present invention easily facilitate understanding, With reference to embodiment to of the present invention
Technical solution is described further, but the present invention is not limited only to this.
Embodiment 1
A method of using food galactan Zhuo Beiershi Flavobacterium degradation sodium alginate and agar composite membrane comprising following
Step:
1) by 5 parts by weight peptones, 1 parts by weight yeast extract, 0.1 parts by weight of phosphoric acid high-speed rail, 30 parts by weight NaCl, 1000 parts by weight
Distilled water.It is made into culture solution, is then to cultivate 3 in 7.5,35 DEG C in pH in culture solution by food galactan Zhuo Beiershi Flavobacterium
It, then its absorbance is adjusted to 0.3, food galactan Zhuo Beiershi Flavobacterium culture solution is obtained, it is spare.
2) agar solution of the sodium alginate soln of 75mL1 wt.% and 75mL1 wt.% are mixed, is stirred at 55 DEG C equal
It is to be then placed in baking oven in 190mm culture dish and dry 12h taking-up at 50 DEG C, add containing 0.5 wt % chlorine that diameter is poured into after even
Change the mixed solution of calcium and 20vt% dehydrated alcohol, solution submerges film, baking oven drying is put into after crosslinking 30min, suitable size is made
Sequin, and weighed with assay balance, weight 0.1104g is spare.
3) the resulting sequin of step 2 is put into food galactan Zhuo Beiershi Flavobacterium culture solution obtained by step 1)
Degradation, takes out after placing 5 days in shaking table at 35 DEG C, film is come out with 200 mesh screens, is washed with distilled water 5 times, chilled
Drying machine is freeze-dried into dry film.Sodium alginate and agar composite membrane after being degraded, and weighed with assay balance,
Weight is 0.0553, degradation rate 49.91%, has good membrane degradation effect.
Embodiment 2
A method of using food galactan Zhuo Beiershi Flavobacterium degradation sodium alginate and agar composite membrane comprising following
Step:
1) by 5 parts by weight peptones, 1 parts by weight yeast extract, 0.1 parts by weight of phosphoric acid high-speed rail, 30 parts by weight NaCl, 1000 parts by weight
Distilled water.It is made into culture solution, is then to cultivate 2 in 7.5,35 DEG C in pH in culture solution by food galactan Zhuo Beiershi Flavobacterium
It, then its absorbance is adjusted to 0.3, food galactan Zhuo Beiershi Flavobacterium culture solution is obtained, it is spare.
2) agar solution of the sodium alginate soln of 75mL1wt.% and 75mL1 wt.% are fallen after mixing evenly at 55 DEG C
Enter diameter be 190mm culture dish in, be then placed in baking oven is dried at 50 DEG C 12h taking-up, add calcium chloride containing 0.5wt.% with
The mixed solution of 20vt% dehydrated alcohol, solution submerge film, are put into the roundlet that suitable size is made in baking oven drying after being crosslinked 30min
Piece, and being weighed with assay balance, weight 0.1034g are spare.
3) the resulting sequin of step 2 is put into food galactan Zhuo Beiershi Flavobacterium culture solution obtained by step 1)
Degradation, takes out after placing 5 days in shaking table at 35 DEG C, film is come out with 200 mesh screens, is washed with distilled water 4 times, chilled
Drying machine is freeze-dried into dry film.Sodium alginate and agar composite membrane after being degraded, and weighed with assay balance,
Weight is 0.0554, degradation rate 46.42%, has good membrane degradation effect.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (2)
1. a kind of method using food galactan Zhuo Beiershi Flavobacterium degradation sodium alginate and agar composite membrane, feature exist
In: the following steps are included:
1) will eat galactan Zhuo Beiershi Flavobacterium is to cultivate 2-3 days in 35 DEG C of culture solution in temperature, then by its absorbance
It is adjusted to 0.3, obtains food galactan Zhuo Beiershi Flavobacterium culture solution, it is spare
2) agar solution of 1 wt.% of the sodium alginate soln of 1 wt.% of 75mL and 75mL is mixed, is stirred evenly at 55 DEG C
After pour into diameter be 190mm culture dish in, be then placed in baking oven is dried at 50 DEG C 12h take out, add containing 0.5 wt.% chlorination
The mixed solution of calcium and 20vt.% dehydrated alcohol, solution submerge film, baking oven drying are put into after crosslinking 30min, sequin is made, standby
With
3) the resulting sequin of step 2 is put into obtained by step 1) and is obtained in food galactan Zhuo Beiershi Flavobacterium culture solution
Degradation, takes out after placing 5 days in shaking table at 35 DEG C, film is come out with 200 mesh screens, is washed with distilled water 3-5 times, through cold
Lyophilizer is freeze-dried into dry film to get the sodium alginate and agar composite membrane to after degrading.
2. according to claim 1 use food galactan Zhuo Beiershi Flavobacterium degradation sodium alginate and agar composite membrane
Method, it is characterised in that: culture solution described in step 1) raw material composition by weight are as follows: 5 parts of peptone, yeast extract 1
Part, 0.1 part of high ferric phosphate, 30 parts of NaCl, 1000 parts of distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811329992.7A CN109438773B (en) | 2018-11-09 | 2018-11-09 | Method for degrading sodium alginate and agar composite membrane by using flavobacterium obesum galactomannan |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811329992.7A CN109438773B (en) | 2018-11-09 | 2018-11-09 | Method for degrading sodium alginate and agar composite membrane by using flavobacterium obesum galactomannan |
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| Publication Number | Publication Date |
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| CN109438773A true CN109438773A (en) | 2019-03-08 |
| CN109438773B CN109438773B (en) | 2020-12-25 |
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| CN201811329992.7A Expired - Fee Related CN109438773B (en) | 2018-11-09 | 2018-11-09 | Method for degrading sodium alginate and agar composite membrane by using flavobacterium obesum galactomannan |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110607266A (en) * | 2019-10-21 | 2019-12-24 | 福州大学 | Flavobacterium for producing alginate lyase and application thereof |
| CN113336336A (en) * | 2021-06-04 | 2021-09-03 | 中国科学院重庆绿色智能技术研究院 | Application of beyerba perniciae in efficient ammonia nitrogen removal |
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- 2018-11-09 CN CN201811329992.7A patent/CN109438773B/en not_active Expired - Fee Related
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110607266A (en) * | 2019-10-21 | 2019-12-24 | 福州大学 | Flavobacterium for producing alginate lyase and application thereof |
| CN110607266B (en) * | 2019-10-21 | 2021-06-01 | 福州大学 | Flavobacterium for producing alginate lyase and application thereof |
| CN113336336A (en) * | 2021-06-04 | 2021-09-03 | 中国科学院重庆绿色智能技术研究院 | Application of beyerba perniciae in efficient ammonia nitrogen removal |
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| CN109438773B (en) | 2020-12-25 |
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