CN109432388B - Composition of probiotics and glucosamine hydrochloride and application of composition in bone joint health - Google Patents
Composition of probiotics and glucosamine hydrochloride and application of composition in bone joint health Download PDFInfo
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- CN109432388B CN109432388B CN201811594602.9A CN201811594602A CN109432388B CN 109432388 B CN109432388 B CN 109432388B CN 201811594602 A CN201811594602 A CN 201811594602A CN 109432388 B CN109432388 B CN 109432388B
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- glucosamine hydrochloride
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Classifications
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
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Abstract
The invention provides a composition of probiotics and glucosamine hydrochloride and application thereof in bone joint health, and relates to the technical field of probiotics. The composition of the probiotics and the glucosamine hydrochloride comprises the following components in parts by weight: 1-5 parts of lactobacillus plantarum LP-Onlly, 5161-5 parts of bifidobacterium lactis BI 5161-5 parts of bifidobacterium longum BL88-Onlly, 20-40 parts of glucosamine hydrochloride, 20-40 parts of milk mineral salt, 1-10 parts of oligosaccharide and 1-10 parts of casein phosphopeptide. The composition of the probiotics and the glucosamine hydrochloride has the functions of increasing bone density and promoting calcium absorption rate, and can be used for preparing corresponding food, medicines or health-care products.
Description
Technical Field
The invention belongs to the technical field of probiotics, and particularly relates to a composition of probiotics and glucosamine hydrochloride and application of the composition in bone and joint health.
Background
Osteoporosis (OP) is a systemic metabolic disease of bone characterized by low bone mass and microstructural destruction of bone tissue, leading to increased bone fragility and susceptibility to fracture, common in the elderly, but can occur at all ages. The Chinese white paper for preventing and treating osteoporosis indicates that at least 6944 million people in China have osteoporosis, 2.1 million people have low bone mass and have osteoporosis risk, 70-80% of middle-aged and old people fracture is caused by osteoporosis, wherein about 181 million people have new vertebral fracture every year, and 23 million cases of hip fracture occur. Researches show that the incidence rate of osteoporosis is 54-71% in 55-65 years old; the prevalence rate for elderly is 60.72% for men and 90.47% for women. With the advent of aging society, osteoporosis has become a high-incidence disease threatening the health of middle-aged and elderly people.
Bone density refers to the bone mineral content per unit volume and is an important marker of bone quality. The more mineral deposits, the greater the bone density and the firmer the bone. Bone density is the main standard for diagnosing osteoporosis, and the world health organization defines that osteoporosis is the condition that the bone density of an adult female is lower than the average value by 2.5 standard deviations.
For osteoporosis, calcium carbonate, calcium gluconate, calcium lactate and the like are common commercially available calcium supplements, which only provide mineral substances mainly including calcium, cannot solve the problems of calcium loss and bone mineralization promotion, and cannot determine whether the calcium supplements can be absorbed or not and how much the calcium supplements can be absorbed.
Disclosure of Invention
In view of the above, the present invention aims to provide a composition of probiotics and glucosamine hydrochloride and its application in bone and joint health, which can increase bone density, supplement calcium and promote calcium absorption, and is used for maintaining joint health.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a composition of probiotics and glucosamine hydrochloride, which comprises the following components in parts by weight: 1-5 parts of lactobacillus plantarum LP-Onlly, 5161-5 parts of bifidobacterium lactis BI 5161-5 parts of bifidobacterium longum BL88-Onlly, 20-40 parts of glucosamine hydrochloride, 20-40 parts of milk mineral salt, 1-10 parts of oligosaccharide and 1-10 parts of casein phosphopeptide.
Preferably, the oligosaccharides comprise: one or more of fructo-oligosaccharide, xylo-oligosaccharide, isomalto-oligosaccharide and galacto-oligosaccharide.
Preferably, the composition further comprises 2-9 parts by weight of auxiliary materials.
Preferably, the auxiliary materials include: maltodextrin, starch, lactose, anhydrous glucose, magnesium stearate and hypromellose.
The invention also provides application of the composition in preparing food for increasing bone density.
The invention also provides application of the composition in preparing a medicament for increasing bone density.
The invention also provides application of the composition in preparing health-care products for increasing bone density.
The invention also provides application of the composition in preparing food for promoting calcium absorption.
The invention also provides application of the composition in preparation of a medicine for promoting calcium absorption.
The invention also provides application of the composition in preparing health-care products for promoting calcium absorption.
The invention provides a composition of probiotics and glucosamine hydrochloride, which is prepared by compounding lactobacillus plantarum LP-Onlly, bifidobacterium lactis BI516, bifidobacterium longum BL 88-Only, glucosamine hydrochloride, milk mineral salt, oligosaccharide, casein phosphopeptide and auxiliary materials, and by probiotics with positive promotion effect on the absorption of mineral elements, the synthesis of vitamins and phytase is promoted, so that the phytic acid effect is inhibited, and the absorption of calcium is promoted while mineral substances are released; in addition, the probiotics can also produce short chain fatty acids, and the absorption of calcium is improved through the dissolving effect of the short chain fatty acids. In the composition, glucosamine hydrochloride is used for protecting cartilage and maintaining joint health, milk mineral salt is used for supplementing important nutrients for calcium and bone growth, probiotics is used for promoting calcium absorption, and casein phosphopeptide and other ingredients are supplemented after the three are compounded, so that the composition can be used for developing safe and effective food, medicine or health-care products with the functions of maintaining joint health, increasing bone density, supplementing calcium and promoting calcium absorption.
Deposit description
Lactobacillus plantarum (Lactobacillus plantarum), with the strain number LP-Onlly, was deposited in China general microbiological culture Collection center (CGMCC) at 06.12.2004 at the address of No. 3, Xilu-1, Beijing, the sunward, and the institute for microbiology, China academy of sciences, with the biological deposition number CGMCC No. 1258.
Bifidobacterium lactis (Bifidobacterium lactis) with strain number BI516, and is preserved in China general microbiological culture Collection Center (CCM) at 04 th 2016, specifically No. 3 of West Lu No.1 of Beijing Kogyo, and No.11959 of China academy of sciences (Microbiol research institute) in Cynomorium sunward.
Bifidobacterium longum (Bifidobacterium longum) with strain number BL88-Onlly, was deposited in China general microbiological culture Collection center at 13.07.2007 with the address of Beijing university Hokkaido No.1, West Lu No. 3, the south Kogyo area, Beijing, and the microbiological research institute of Chinese academy of sciences with the biological preservation number CGMCC No. 2107.
Detailed Description
The invention provides a composition of probiotics and glucosamine hydrochloride, which comprises the following components in parts by weight: 1-5 parts of lactobacillus plantarum LP-Onlly, 5161-5 parts of bifidobacterium lactis BI 5161-5 parts of bifidobacterium longum BL88-Onlly, 20-40 parts of glucosamine hydrochloride, 20-40 parts of milk mineral salt, 1-10 parts of oligosaccharide and 1-10 parts of casein phosphopeptide.
The composition comprises lactobacillus plantarum LP-Onlly, and the preferable weight part of the lactobacillus plantarum LP-Onlly in the composition is 3-5 parts. The Lactobacillus plantarum (Lactobacillus plantarum) LP-Onlly of the invention is preserved in China general microbiological culture Collection center (CGMCC) at 12.2004 and 06.12.h, with the address of No. 3 Xilu-Shih-1 of Beijing Korean district, and the number of biological preservation of the institute of microbiology of China academy of sciences is CGMCC No. 1258.
The composition comprises bifidobacterium lactis BI516, and the weight part of the bifidobacterium lactis BI516 in the composition is preferably 3-5 parts. The Bifidobacterium lactis BI516 is preserved in the common microorganism center of China Committee for culture Collection of microorganisms at 2016, 01, 04, with the address of No. 3, West Lu No.1, North Chen, of the sunward area in Beijing, and the number of biological preservation is CGMCC No.11959, which is the institute of microbiology of the Chinese academy of sciences.
The composition comprises bifidobacterium longum BL88-Onlly, and the preferable weight part of the bifidobacterium longum BL88-Onlly in the composition is 3-5 parts. The Bifidobacterium longum (Bifidobacterium longum) BL88-Onlly is preserved in China general microbiological culture Collection center at 13 th month in 2007 with the address of No. 3 of No.1 of West Lu of Beijing Korean district, and the microbiological collection number of the China academy of sciences is CGMCC No. 2107.
In the invention, the lactobacillus plantarum LP-Onlly, the bifidobacterium lactis and the bifidobacterium longum BL88-Onlly can promote the synthesis of vitamin D through multiple mechanisms, improve the utilization rate of mineral substances, increase the level of blood calcium and increase the bone mineral density and the bone mineral content, and the strain has high activity and strong stability and can play an auxiliary role in promoting the absorption of calcium.
The composition comprises glucosamine hydrochloride, and the weight part of the glucosamine hydrochloride in the composition is preferably 25-35 parts. The glucosamine hydrochloride can reduce the symptoms of joint swelling, joint stiffness and the like caused by osteoarthritis or rheumatic arthritis, and greatly improve the life quality of arthritis patients. In the present invention, the source of the glucosamine hydrochloride is not particularly limited, and a conventional reagent in the art may be used.
The composition comprises milk mineral salt, and the weight part of the mineral salt in the composition is preferably 30-40 parts. The milk mineral salt of the present invention is preferably milk calcium. The milk mineral salt is a new resource food, is prepared by taking whey as a raw material and removing components such as protein, lactose and the like, contains 23-28% of calcium, contains phosphorus, magnesium, potassium, iron, zinc, copper and other trace elements with the same proportion as human bones, and has a protective effect on the bones. The source of the milk mineral salt is not particularly limited in the present invention, and conventional reagents in the art may be used.
The composition comprises oligosaccharide, and the weight part of the oligosaccharide in the composition is preferably 5-8 parts. The oligosaccharide of the present invention preferably comprises: one or more of fructo-oligosaccharide, xylo-oligosaccharide, isomalto-oligosaccharide and galacto-oligosaccharide. The oligosaccharide can be used as a proliferation factor of beneficial bacteria in intestinal tracts, and has an obvious effect on the proliferation of bifidobacteria; the Bifidobacterium has health promoting effect on human body, such as improving vitamin metabolism, wherein the metabolism of vitamin D, K is closely related to calcium metabolism.
The composition comprises casein phosphopeptide, and the weight part of the casein phosphopeptide in the composition is preferably 3-7 parts. The casein phosphopeptide takes cow milk casein as a raw material, and the bioactive peptide obtained by enzymatic hydrolysis, separation and purification has the capability of chelating calcium. Can be combined with divalent mineral ions such as calcium, iron, magnesium, zinc, selenium, etc. in small intestine environment to prevent phosphate precipitation, and enhance the concentration of soluble minerals in intestine, thereby promoting absorption and utilization. The casein phosphopeptide in the composition can combine and transport calcium in small intestine, is equivalent to a medium-short distance transportation protection tool of calcium, avoids the loss of calcium in the process of circulation, and promotes the absorption and utilization of calcium.
The composition comprises an auxiliary material, and the weight part of the auxiliary material in the composition is preferably 4.5-9 parts. The auxiliary materials of the invention preferably comprise: maltodextrin, starch, lactose, anhydrous glucose, magnesium stearate and hypromellose. The auxiliary materials can be used for adjusting the adhesiveness, the fluidity and the like of materials in the filling and tabletting processes of all components in the composition, for example, hydroxypropyl methylcellulose is a tablet premix coating material.
The invention also provides application of the composition in preparing food for increasing bone density. The food of the invention is preferably in the form of tablets, capsules or powders. The preparation method of the food is not particularly limited, and the conventional preparation method in the field can be used.
The invention also provides application of the composition in preparing a medicament for increasing bone density. The dosage form of the medicament of the invention is preferably tablets, capsules or powder. The preparation method of the medicine is not particularly limited, and the conventional preparation method in the field can be used.
The invention also provides application of the composition in preparing health-care products for increasing bone density. The dosage form of the health care product is preferably tablets, capsules or powder. The preparation method of the health care product is not particularly limited, and the conventional preparation method in the field can be utilized.
The invention also provides application of the composition in preparing food for promoting calcium absorption. The food of the invention is preferably in the form of tablets, capsules or powder. The preparation method of the food is not particularly limited, and the conventional preparation method in the field can be used.
The invention also provides application of the composition in preparation of a medicine for promoting calcium absorption. The dosage form of the medicament is preferably tablets, capsules or powder. The preparation method of the medicine is not particularly limited, and the conventional preparation method in the field can be used.
The invention also provides application of the composition in preparing health-care products for promoting calcium absorption. The dosage form of the health care product is preferably tablets, capsules or powder. The preparation method of the health care product is not particularly limited, and the conventional preparation method in the field can be utilized.
The composition of probiotic bacteria and glucosamine hydrochloride and the application thereof in bone joint health provided by the present invention will be described in detail with reference to the following examples, which should not be construed as limiting the scope of the present invention.
Example 1
The components are compounded into a composition according to the following weight percentage: lactobacillus plantarum LP-Onlly 2kg, bifidobacterium lactis BI 5165 kg, bifidobacterium longum BL88-Onlly 2kg, glucosamine hydrochloride 40kg, milk mineral salt 40kg, fructo-oligosaccharide 1kg, casein phosphopeptide 4kg, maltodextrin 5.5kg and magnesium stearate 0.5 kg.
The following experiment was carried out using the probiotic and glucosamine hydrochloride composition prepared in example 1 as a sample:
experiment 1: experiment for increasing bone Density
Samples and materials: using the composition of example 1 as a sample, the recommended amount for the human body of the composition is 3.78 g/day 60kg per day. The sample contained 11.3% calcium. 70 female adult rats (cleaning grade SD rats) 250-288 g are selected in the experiment. The temperature of the breeding room is 20-25 ℃, and the relative humidity is 40-70%.
Rats are anesthetized by intraperitoneal injection of 30 mg/kg-bw sodium pentobarbital solution, hairs at the positions of dorsal costal ridges are removed, iodine tincture and alcohol are respectively used for sterilization, bilateral ovaries are cut off, and another group of animals are taken for the same operation without cutting the bilateral ovaries and are used as a sham operation control group. 5 days after ovariectomy of the rats, vaginal smear examination was performed to eliminate the ovariectomized rats.
Dose design: according to the requirements of experimental items, three dosage groups of low, medium and high are set, namely 0.32, 0.63 and 1.89g/kg · bw, which are equivalent to 5 times, 10 times and 30 times of the recommended dosage of a human body. The rats after the operation are randomly divided into a model control group, a positive control group (1.0 mg/kg-bw estradiol), a calcium carbonate control group (the calcium level is the same as the high-dose calcium level) and a low, medium and high dose group according to the weight, wherein each group comprises 10 rats, and 10 sham-operated rats are taken as the sham-operated control group.
Preparing a test substance: samples 0.32, 0.63 and 1.89g are respectively taken, deionized water is added to 10mL, and test solutions of low, medium and high dosage groups are uniformly prepared; 534mg of calcium carbonate is taken, deionized water is added to 10mL, and the calcium carbonate is uniformly mixed to prepare a calcium carbonate control group test solution; deionized water was given to both the sham-operated group and the model control group. Each group of drinking water was deionized water.
Subject administration mode: the samples were administered by gavage with a gavage volume of 10mL/kg body weight. Gavage was performed once daily, each group of doses was designed to be given for 12 weeks, and the rats were given deionized water throughout the duration of the experiment, and body weights were recorded weekly, during which time the experimental rat feed formulations were as shown in table 1:
table 1 reference feed formulation
Note: 1. contains per kg of mixed mineral salts (g): potassium dihydrogen phosphate, 250.0; sodium chloride, 74.0; 46.6 parts of potassium sulfate; potassium citrate (mono-crystalline water), 28.0; magnesium oxide, 24.0; 6.06 parts of citric acid; zinc carbonate, 1.65; 0.63 parts of manganese carbonate; 0.30 parts of copper carbonate; potassium iodide, 0.01; sodium selenate, 0.01025; para-molybdate, 0.00795; sodium metasilicate (9 crystal waters), 1.45; chromium potassium sulfate (12 crystal waters), 0.275; boric acid, 0.0815; sodium fluoride, 0.0635; nickel carbonate, 0.318; lithium chloride 0.0174; ammonium vanadate, 0.0066; sucrose was added to 1 kg.
2. The vitamin composition contains (g) per kg of mixed vitamins: nicotinic acid, 3.0; calcium pantothenate, 1.6; pyridoxine hydrochloride, 0.7; thiamine hydrochloride, 0.60; vitamin B2, 0.60; folic acid, 0.2; biotin, 0.02; vitamin B12, 2.5; vitamin E, 15.0; 0.8 parts of vitamin A; vitamin D3, 0.25; vitamin K, 0.075; sucrose 974.655.
The results of measuring the effect of the composition described in example 1 on the body weight of rats are shown in table 2:
TABLE 2 Effect of samples on rat body weight
Note: indicates that the difference between each group in the same column and the comparison group of the model control group is significant, and P <0.05
As can be seen from Table 2, the average body weight and weight gain of the control group after four weeks are significantly higher than those of the control group after the false operation, which suggests that abnormal increase of body weight caused by hormone level disorder due to estrogen reduction of the animals after the operation may occur, indicating that the model is established. The average body weight in the middle and four weeks, the average body weight in the later four weeks and the weight gain of the positive control group are all obviously lower than those of the castration model control group. Compared with the model control group, the initial weight, the average weight per four weeks and the weight gain of each dose group have no obvious difference (P > 0.05). Compared with the calcium carbonate control group, the high-dose group has no significant difference in initial body weight, average body weight per four weeks and weight gain (P > 0.05).
Measuring the bone density of the femur: the rats were dissected, the left femur was dissected and baked to constant weight. The bone density of the midpoint of the femur and the distal end of the femur of a rat are measured by using a discovery-wi type bone densitometer, and the bone calcium content is measured by using an atomic absorption method, wherein the results are shown in table 3:
TABLE 3 Effect of samples on rat femoral weight, bone calcium and femoral bone Density
Note: indicates that the difference between each group in the same column and the model control group compared with each group is significant (P <0.05)
As can be seen from table 3, the indexes of the positive control group and the sham-operated control group, except for the dry weight of the femur, are significantly higher than those of the model control group. Compared with a model control group, the bone density and the bone calcium content of the distal end of the femur of the high-dose group and the calcium carbonate control group are obviously increased, and compared with the calcium carbonate control group with the same calcium level, the high-dose group has no significant difference (P is more than 0.05) in each index.
Under the experimental condition, the bone calcium content and the bone density value of the model control group are obviously lower than those of the sham operation group and the positive control group; the bone calcium content and the bone density value at the distal end of the femur of the high-dose group of the test object group are obviously higher than those of the model control group, and the composition can be considered to have the function of increasing the bone density of rats.
Experiment 2: calcium absorption test
Samples and materials: using the composition of example 1 as a sample, the recommended amount for the human body of the composition is 3.78 g/day 60kg per day. The sample contained 11.3% calcium. Weaning rats (cleaning grade SD rats) 60-79 g, 50 of 4 weeks after birth. The temperature of the breeding room is 20-25 ℃, and the relative humidity is 40-70%.
Dose design: the low, medium and high dose groups are set, namely 0.32, 0.63 and 1.89g/kg · bw, which are equivalent to 5 times, 10 times and 30 times of the recommended dose of a human body. A low calcium control and a calcium carbonate control (calcium level and high dose calcium level were the same) were established simultaneously.
Preparing a test substance: samples 0.32, 0.63 and 1.89g are respectively taken, deionized water is added to 10mL, and test solutions of low, medium and high dosage groups are uniformly prepared; 534mg of calcium carbonate is taken and added with deionized water to 10mL, and the calcium carbonate is evenly mixed to prepare a calcium carbonate control group test solution. Each group of drinking water was deionized water.
Subject administration mode: the samples were administered by gavage with a gavage volume of 10mL/kg body weight.
Calcium absorption experimental method
a, sample collection:
weaning rats born for 4 weeks were housed in cages. The rats were randomly divided into a low calcium control group, a calcium carbonate control group, and low, medium, and high dose groups according to body weight, each group consisting of 10 rats. Samples were gavaged to each group and the low calcium control group was given deionized water for 4 weeks. Body length and body weight were measured once a week. Calcium metabolism experiments were performed 3 days after experiment 3 weeks. Food intake was recorded for 3 days, feces were collected for 72 hours, and calcium content in the feces was measured. And drying the rat manure sample in an oven at the temperature of 80 ℃, cooling the rat manure sample in a dryer, and finely grinding the rat manure sample. Care was taken to prevent contamination during sample preparation. The feed formulation for the test rats during this period is shown in table 4:
TABLE 4 basic feed formulation (adjustment of Ca)2+Is 150mg/100g feed)
Note: 1. contains per kg of mixed mineral salts (g): potassium dihydrogen phosphate, 250.0; sodium chloride, 74.0; magnesium carbonate, 50.2; ferrous lactate, 5.4; zinc lactate, 4.16; 3.5 parts of manganese carbonate; 0.605 parts of copper sulfate pentahydrate; sodium selenate, 0.0066; potassium iodide, 0.00776; chromium trichloride hexahydrate, 0.292; sucrose was added to 1 kg.
2. The vitamin composition contains (g) per kg of mixed vitamins: vitamin a, 400,000 IU; vitamin D3, 100,000 IU; vitamin E, 500 IU; vitamin K, 5 mg; vitamin B1, 600 mg; vitamin B2, 600 mg; vitamin B6, 700 mg; vitamin B12, 1 mg; nicotinic acid, 3 g; folic acid, 200 mg; 1.6g of calcium pantothenate; biotin, 20 mg; sucrose was added to 1 kg.
b, measuring the bone calcium content: and (4) measuring by an atomic absorption method.
c apparent absorption rate of calcium:
calcium intake (mg) ═ feed calcium content (mg/g) × 3 days feed consumption + sample calcium intake
Calcium content (mg/g) in feces x 3 days feces excretion
Apparent absorption rate (%) of calcium (calcium intake-faecal calcium)/calcium intake × 100%
The results of the experiment are shown in table 5:
TABLE 5 Effect of samples on calcium absorption in the Experimental period of rats
Note: indicates that the difference between each group in the same column and the model control group compared with each group is significant (P <0.05)
As can be seen from Table 5, there was no apparent difference in body weight and body length between the dose groups compared with the low calcium control group (P > 0.05). The apparent absorption rate of calcium in the high dose group was significantly higher than that in the low calcium control group.
Example 2
The components are compounded into a composition according to the following weight ratio: 3kg of lactobacillus plantarum LP-Onlly, 3kg of bifidobacterium lactis BI 5165 kg, 5kg of bifidobacterium longum BL88-Onlly, 35kg of glucosamine hydrochloride, 40kg of milk mineral salt, 6kg of xylooligosaccharide, 4kg of casein phosphopeptide and 2kg of anhydrous glucose.
Using the composition obtained in example 2, the same bone density increasing experiment and calcium absorption experiment as in example 1 were performed, wherein the effect of the samples on the body weight of rats is shown in table 6:
TABLE 6 Effect of samples on rat body weight
Note: indicates that the difference between each group in the same column and the comparison group of the model control group is significant, and P <0.05
As can be seen from Table 6, the average body weight and weight gain of the control group in the middle and four weeks after the operation of the model are significantly higher than those of the control group in the false operation, which suggests that abnormal increase of body weight caused by hormone level disorder due to estrogen reduction of the animals after the operation is possible, and indicates that the model is established. The average body weight in the middle and four weeks, the average body weight in the later four weeks and the weight gain of the positive control group are all obviously lower than those of the castration model control group. Compared with the model control group, the initial weight, the average weight per four weeks and the weight gain of each dose group have no obvious difference (P > 0.05). Compared with the calcium carbonate control group, the high-dose group has no significant difference in initial body weight, average body weight per four weeks and weight gain (P > 0.05).
The results of the effect of the samples on rat femoral weight, bone calcium and femoral bone density are shown in table 7:
TABLE 7 Effect of samples on rat femoral weight, bone calcium and femoral bone Density
Note: indicates that the difference between each group in the same column and the model control group compared with each group is significant (P <0.05)
As can be seen from table 7, the indexes of the positive control group and the sham-operated control group, except for the dry weight of the femur, are significantly higher than those of the model control group. Compared with a model control group, the bone density and the bone calcium content of the distal end of the femur of the high-dose group and the calcium carbonate control group are obviously increased, and compared with the calcium carbonate control group with the same calcium level, the high-dose group has no significant difference (P is more than 0.05) in each index.
Under the experimental condition, the bone calcium content and the bone density value of the model control group are obviously lower than those of the sham operation group and the positive control group; the bone calcium content and the bone density value at the distal end of the femur of the high-dose group of the test object group are obviously higher than those of the model control group, and the composition can be considered to have the function of increasing the bone density of rats.
The results of the calcium absorption experiments are shown in table 8:
TABLE 8 Effect of samples on calcium absorption in the Experimental period of rats
Note: indicates that the difference between each group in the same column and the model control group compared with each group is significant (P <0.05)
As can be seen from Table 8, there was no apparent difference in body weight and body length between the dose groups compared with the low calcium control group (P > 0.05). The apparent absorption rate of calcium in the medium-dose and high-dose groups is obviously higher than that in the low-calcium control group.
Example 3
The components are compounded into a composition according to the following weight ratio: 4kg of lactobacillus plantarum LP-Onlly, 4kg of bifidobacterium lactis BI 5163 kg, 2kg of bifidobacterium longum BL88-Onlly, 25kg of glucosamine hydrochloride, 40kg of milk mineral salt, 7kg of xylooligosaccharide, 10kg of casein phosphopeptide, 5kg of anhydrous glucose and 4kg of maltodextrin.
Using the composition obtained in example 3, the same bone density increasing experiment and calcium absorption experiment as in example 1 were conducted, wherein the effect of the samples on the body weight of rats is shown in Table 9:
TABLE 9 Effect of samples on rat body weight
Note: indicates that the difference between each group in the same column and the comparison group of the model control group is significant, and P <0.05
As can be seen from Table 9, the average body weight and weight gain of the control group in the middle and four weeks after the operation of the model are significantly higher than those of the control group in the false operation, which suggests that abnormal increase of body weight caused by hormone level disorder due to estrogen reduction of the animals after the operation may occur, indicating that the model is established. The average body weight in the middle and four weeks, the average body weight in the later four weeks and the weight gain of the positive control group are all obviously lower than those of the castration model control group. Compared with the model control group, the initial weight, the average weight per four weeks and the weight gain of each dose group have no obvious difference (P > 0.05). Compared with the calcium carbonate control group, the high-dose group has no significant difference in initial body weight, average body weight per four weeks and weight gain (P > 0.05).
The results of the effect of the samples on rat femoral weight, bone calcium and femoral bone density are shown in table 10:
TABLE 10 Effect of samples on rat femoral weight, bone calcium and femoral bone Density
Note: indicates that the difference between each group in the same column and the model control group compared with each group is significant (P <0.05)
As can be seen from table 10, the indexes of the positive control group and the sham-operated control group, except for the dry weight of the femur, are significantly higher than those of the model control group. Compared with a model control group, the bone density and the bone calcium content of the distal end of the femur of the high-dose group and the calcium carbonate control group are obviously increased, and compared with the calcium carbonate control group with the same calcium level, the high-dose group has no significant difference (P is more than 0.05) in each index.
Under the experimental condition, the bone calcium content and the bone density value of the model control group are obviously lower than those of the sham operation group and the positive control group; the bone calcium content and the bone density value at the distal end of the femur of the high-dose group of the test object group are obviously higher than those of the model control group, and the composition can be considered to have the function of increasing the bone density of rats.
The results of the calcium absorption experiments are shown in table 11:
TABLE 11 Effect of samples on calcium absorption in the Experimental period of rats
Note: indicates that the difference between each group in the same column and the model control group compared with each group is significant (P <0.05)
As can be seen from Table 11, there was no apparent difference in body weight and body length between the dose groups compared with the low calcium control group (P > 0.05). The apparent absorption rate of calcium in the high dose group was significantly higher than that in the low calcium control group.
In conclusion, the invention provides a composition of probiotics and glucosamine hydrochloride, which has the functions of increasing bone density and promoting calcium absorption rate and can be used for preparing corresponding food, medicines or health-care products in bone joint health.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. The composition of probiotics and glucosamine hydrochloride is characterized by comprising the following components in parts by weight: 2 parts of lactobacillus plantarum LP-Onlly, 2 parts of bifidobacterium lactis BI 5165 parts, 2 parts of bifidobacterium longum BL88-Onlly, 40 parts of glucosamine hydrochloride, 40 parts of milk mineral salt, 1 part of fructo-oligosaccharide, 4 parts of casein phosphopeptide and 6 parts of auxiliary materials;
the auxiliary materials comprise: maltodextrin and magnesium stearate, wherein the weight part ratio of the maltodextrin to the magnesium stearate is 5.5: 0.5.
2. Use of the composition of claim 1 for the preparation of a food product for increasing bone density.
3. Use of a composition according to claim 1 for the manufacture of a medicament for increasing bone density.
4. Use of the composition of claim 1 for the preparation of a health product for increasing bone density.
5. Use of the composition of claim 1 for the preparation of a food product for promoting calcium absorption.
6. Use of a composition according to claim 1 for the manufacture of a medicament for promoting calcium absorption.
7. Use of the composition of claim 1 for the preparation of a health product for promoting calcium absorption.
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