CN109430526A - A kind of lactalbumin Se-enriched bio feed and its application - Google Patents
A kind of lactalbumin Se-enriched bio feed and its application Download PDFInfo
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Abstract
The present invention relates to feed processing technologies; in particular to a kind of lactalbumin Se-enriched bio feed and its application; through applicants have found that; lactic acid bacterium source selenium is by lactic acid bacteria and selenium element chelating; greater activity can be kept in the animal gastric juice of highly acidity; it can play the role of protecting animal intestinal tract, meanwhile, the milk being discharged through animal's mammary gland is the highest part of selenium conversion ratio in entire animal body；Therefore the application researchs and develops a kind of Se-enriched feedstuff, and the feed is by whey powder, bean powder, indocalamus leaf, safflower, enzyme preparation and lactic acid bacterium source organic selenium solution composition, wherein bean powder is made of soybean powder, black bean powder and broad bean powder；Enzyme preparation is made of compound protease and carboxypeptidase；Its reasonable composition has good synergistic effect, can effectively protect animal intestinal tract, mammary gland function, improves mammal to the conversion ratio of selenium.
Description
[technical field]
The present invention relates to feed processing technologies, and in particular to a kind of lactalbumin Se-enriched bio feed and its application.
[background technique]
Selenium element bioactivity with higher has and removes interior free yl, delays senescence, improves cellular anti-oxidant
Effect, praised highly in recent years by food, medicine trade, selenium is divided into vegetable active selenium and two kinds of inorganic selenium, inorganic selenium one again
As refer to sodium selenite and sodium selenate, include the remaining yeast selenium of a large amount of inorganic seleniums, malt selenium, from the byproduct of metal mineral reserves
It obtains, inorganic selenium has biggish toxicity, and is not easy to be absorbed, and is not suitable for humans and animals and uses.Vegetable active selenium is turned by biology
Change be combined into amino acid, generally in the form of selenomethionine presence, vegetable active selenium be human and animal allow using
Selenium source.Currently used organic selenium source is yeast selenium, yet there are no the relevant report of lactic acid bacteria selenium source；And lactic acid bacteria is a kind of
Beneficial bacteria of intestinal tract can play the role of balance intestinal flora, improve intestinal immunity, and intestinal tract immune system is that animal is maximum
Immune system, the raising of intestinal immunity help to improve animal body immunity.
However, how improving selenium conversion ratio during utilization of the animal body to organic selenium, reducing selenium toxicity, be
Our technical problems urgently to be resolved.
[summary of the invention]
In view of the above-mentioned problems, the technical problem to be solved in the present invention is to provide a kind of lactalbumin Se-enriched bio feed and its
Using, reach animal body to organic selenium utilization during, how to improve selenium conversion ratio, reduce selenium toxicity effect.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
A kind of lactalbumin Se-enriched bio feed, the feed are made of following parts by weight ingredient: 45 parts -57 parts of whey
Powder, 36 parts -43 parts of bean powder, 18 parts -27 parts of indocalamus leaf, 13 parts -24 parts of safflower, 1 part -3 parts of enzyme preparation and 2 parts -5 parts
Prebiotic bacterium source organic selenium solution.
Further, the bean powder is made of soybean powder, black bean powder and broad bean powder according to mass ratio for 8-10:2-4:1-3.
Further, the enzyme preparation is by the compound protease of 150U/mg-200U/mg and the carboxylic of 200U/mg-250U/mg
Peptase is 1:3-4 composition according to mass ratio.
Further, the prebiotic bacterium source organic selenium solution is lactic acid bacterium source organic selenium solution, and preparation method is will have
Imitating viable count is 2.2 × 105-5.2×105The lactic acid bacteria of cfu/mg is accessed according to the inoculum concentration of 1%-3% containing the concentrated liquid of tradition Chinese medicine
Fluid nutrient medium, temperature be 35-38 DEG C at Anaerobic culturel 70h-72h to obtain the final product.
Further, the fluid nutrient medium containing the concentrated liquid of tradition Chinese medicine are as follows: the Radix Isatidis of 1g/L-3g/L, 1g/L-3g/L
Pueraria lobata, the aloe of 1g/L-3g/L, the dehydrated potato powder of 10g/L-15g/L, the whey powder of 5g/L-10g/L, 3g/L-5g/L seaweed are more
The phosphoric acid hydrogen of sugar, the ammonium chloride of the gumbo polysaccharide of 2g/L-4g/L, the magnesium sulfate of 1g/L-3g/L, 1g/L-3g/L, 1g/L-3g/L
The sodium selenite of potassium, 1g/L.
The present invention also provides a kind of method for preparing lactalbumin Se-enriched bio feed, the methods are as follows: by weight
Weigh each raw material, will whey powder, bean powder and enzyme preparation mix after to mixture spray 35-37 DEG C of thermostatted water, kneading it is agglomerating
It is digested under conditions of 37 DEG C for 24 hours, then adds indocalamus leaf, safflower and prebiotic bacterium source organic selenium solution and be further continued for 35-37 DEG C of sprinkling
Thermostatted water stir the mixture for into paste, then ferment at constant temperature 5-7 days under conditions of 35-40 DEG C to obtain the final product.
The present invention also provides purposes of the lactalbumin Se-enriched bio feed in protection mammal galactophore function.
Compared with prior art, the invention has the following beneficial effects:
Through applicants have found that, lactic acid bacterium source selenium, can be in the animal gastric juice of highly acidity by lactic acid bacteria and selenium element chelating
Middle holding greater activity can play the role of protecting animal intestinal tract, meanwhile, the milk being discharged through animal's mammary gland is entire animal body
The middle highest part of selenium conversion ratio；Therefore the application research and develop a kind of Se-enriched feedstuff, the feed by whey powder, bean powder, indocalamus leaf,
Safflower, enzyme preparation and prebiotic bacterium source organic selenium solution composition, wherein bean powder is made of soybean powder, black bean powder and broad bean powder；Enzyme system
Agent is made of compound protease and carboxypeptidase；Prebiotic bacterium source organic selenium solution is lactic acid bacterium source organic selenium solution, by will be newborn
In sour fluid nutrient medium of the bacterium access containing the concentrated liquid of tradition Chinese medicine, ferments and be made by Anaerobic culturel；Whey powder be referred to as " albumen it
King " also contains lactose other than protein rich in, and lactose is used as carbon source for lactobacillus-fermented growth, whey powder and its
His protein sources are for origin of amino acid；But digested using only whey powder, aminoacid ingredient is relatively simple, inventor
Research finds that protein sources reuse bean powder and can solve these technical problems, and the bean powder of the application is by soybean powder, black bean powder and semen viciae fabae
Wherein flavones rich in, flavones can protect the ovary of mammal to powder composition, promote the secretion of estrogen, thus further
Promote mammal lactation, improves and the mammary gland function of mammal is protected；Indocalamus leaf and safflower have the function of promoting blood circulation, can be effective
Promote absorption of the mammal to feed nutrition substance；And enzyme preparation used in this application is by compound protease and carboxypeptidase group
At compound protease can effectively decompose multiple proteins, and carboxypeptidase can pass through the direct breaks down into amino acids small molecule of protein
It can promote absorption of the mammal to organic selenium, energy effectively on selenium element chelating to amino acid after accessing selenium source probiotics
Mammal is improved to the conversion ratio of selenium in feed；Prebiotic bacterium source organic selenium solution of the invention is that lactic acid bacterium source organic selenium is molten
Liquid ferments by Anaerobic culturel and is made by accessing lactic acid bacteria in the fluid nutrient medium containing the concentrated liquid of tradition Chinese medicine；Traditional Chinese drug inspissation
Liquid can effectively improve the emulsifying effectiveness, anti-oxidation function and the viable count of lactic acid bacteria of lactic acid bacteria.The viable count quantity of lactic acid bacteria increases
Add, mammal these probiotics in edible feed can enter animal gastrointestinal tract, to adjust the function of intestinal canal of animal, protect
Stomach is easier to by animal edible, absorbs, each element of the application can balance in female mammal body so that feed is more agreeable to the taste
Hormone-content, allow female mammal mammogenesis faster；It can be in terms of dredging mammary gland pipeline, promoting hormonal balance two mutually
Effect is played the role of protecting female mammal mammary gland function, makes to reach and improve mammal to the conversion ratio of selenium and cream
The purpose of Se content in product.
[specific embodiment]
A specific embodiment of the invention is described further with reference to embodiments.
Embodiment 1
The processing method of the present embodiment lactalbumin Se-enriched bio feed the following steps are included:
It (1) is 2.2 × 10 by living bacteria count5The lactic acid bacteria of cfu/mg accesses culture medium prescription according to 1% inoculum concentration
Are as follows: the Radix Isatidis of 1g/L, the pueraria lobata of 1g/L, the aloe of 1g/L, the dehydrated potato powder of 10g/L, the whey powder of 5g/L, 3g/L seaweed are more
Sugar, the gumbo polysaccharide of 2g/L, the magnesium sulfate of 1g/L, the ammonium chloride of 1g/L, the potassium hydrogen phosphate of 1g/L, 1g/L sodium selenite liquid
In body culture medium, wherein the pH value of culture medium is 6.9；Anaerobic culturel 70h obtains prebiotic bacterium source organic selenium at being 35 DEG C in temperature
Solution.
(2) each raw material is weighed by following parts by weight: 45 parts of whey powder, 36 parts of bean powder, 18 parts of indocalamus leaf, 13 parts red
Flower, 1 part of enzyme preparation and 2 parts of prebiotic bacterium source organic selenium solution；Wherein, bean powder by soybean powder, black bean powder and broad bean powder according to
Mass ratio is 8:2:1 composition；Enzyme preparation is 1:3 according to mass ratio by the compound protease of 150U/mg and the carboxypeptidase of 200U/mg
Composition；
(3) 35 DEG C of thermostatted water will be sprayed after the mixing of whey powder, bean powder and enzyme preparation to mixture, kneading is agglomerating at 37 DEG C
Under conditions of digest for 24 hours, then add indocalamus leaf, safflower and prebiotic bacterium source organic selenium solution mixing, stirring, during stirring
The thermostatted water of 35 DEG C of sprinkling is further continued for until stirring the mixture for into paste, is then within ferment at constant temperature 5 days under conditions of 35 DEG C
Obtain the biological feedstuff of the application.
Embodiment 2
The processing method of the present embodiment lactalbumin Se-enriched bio feed the following steps are included:
It (1) is 5.2 × 10 by living bacteria count5The lactic acid bacteria of cfu/mg accesses culture medium prescription according to 3% inoculum concentration
Are as follows: the Radix Isatidis of 3g/L, the pueraria lobata of 3g/L, the aloe of 3g/L, the dehydrated potato powder of 15g/L, the whey powder of 10g/L, 5g/L seaweed
Polysaccharide, the gumbo polysaccharide of 4g/L, the magnesium sulfate of 3g/L, the ammonium chloride of 3g/L, the potassium hydrogen phosphate of 3g/L, 1g/L sodium selenite
In fluid nutrient medium, wherein the pH value of culture medium is 7.8；It is organic to obtain prebiotic bacterium source by Anaerobic culturel 72h at being 38 DEG C in temperature
Selenium solution.
(2) each raw material is weighed by following parts by weight: 57 parts of whey powder, 43 parts of bean powder, 27 parts of indocalamus leaf, 24 parts red
Flower, 3 parts of enzyme preparation and 5 parts of prebiotic bacterium source organic selenium solution；Wherein, bean powder by soybean powder, black bean powder and broad bean powder according to
Mass ratio is 10:4:3 composition；Enzyme preparation is 1 according to mass ratio by the compound protease of 200U/mg and the carboxypeptidase of 250U/mg:
4 compositions；
(3) 37 DEG C of thermostatted water will be sprayed after the mixing of whey powder, bean powder and enzyme preparation to mixture, kneading is agglomerating at 37 DEG C
Under conditions of digest for 24 hours, then add indocalamus leaf, safflower and prebiotic bacterium source organic selenium solution mixing, stirring, during stirring
The thermostatted water of 37 DEG C of sprinkling is further continued for until stir the mixture for into paste, then ferment at constant temperature 7 under conditions of 40 DEG C to obtain the final product
The biological feedstuff of the application.
Embodiment 3
The processing method of the present embodiment lactalbumin Se-enriched bio feed the following steps are included:
It (1) is 3.2 × 10 by living bacteria count5The lactic acid bacteria of cfu/mg accesses culture medium prescription according to 2% inoculum concentration
Are as follows: the Radix Isatidis of 2g/L, the pueraria lobata of 2g/L, the aloe of 2g/L, the dehydrated potato powder of 12g/L, the whey powder of 7g/L, 4g/L seaweed are more
Sugar, the gumbo polysaccharide of 3g/L, the magnesium sulfate of 2g/L, the ammonium chloride of 2g/L, the potassium hydrogen phosphate of 2g/L, 1g/L sodium selenite liquid
In body culture medium, wherein the pH value of culture medium is 7.2；Anaerobic culturel 71h obtains prebiotic bacterium source organic selenium at being 37 DEG C in temperature
Solution.
(2) each raw material is weighed by following parts by weight: 50 parts of whey powder, 40 parts of bean powder, 21 parts of indocalamus leaf, 21 parts red
Flower, 2 parts of enzyme preparation and 3 parts of prebiotic bacterium source organic selenium solution；Wherein, bean powder by soybean powder, black bean powder and broad bean powder according to
Mass ratio is 9:3:2 composition；Enzyme preparation is 1:4 according to mass ratio by the compound protease of 180U/mg and the carboxypeptidase of 220U/mg
Composition；
(3) 36 DEG C of thermostatted water will be sprayed after the mixing of whey powder, bean powder and enzyme preparation to mixture, kneading is agglomerating at 37 DEG C
Under conditions of digest for 24 hours, then add indocalamus leaf, safflower and prebiotic bacterium source organic selenium solution mixing, stirring, during stirring
The thermostatted water of 36 DEG C of sprinkling is further continued for until stirring the mixture for into paste, is then within ferment at constant temperature 6 days under conditions of 37 DEG C
Obtain the biological feedstuff of the application.
Control group 1:
The biological feedstuff of this control group is raw material without using whey powder, and raw material comprises the following components in parts by weight: 36
The bean powder, 18 parts of indocalamus leaf, 13 parts of safflower, 1 part of enzyme preparation and 2 parts of prebiotic bacterium source organic selenium solution of part；Wherein, bean powder
It is made of soybean powder, black bean powder and broad bean powder according to mass ratio for 8:2:1；Enzyme preparation by 150U/mg compound protease and
The carboxypeptidase of 200U/mg is 1:3 composition according to mass ratio, and other embodiment and embodiment 1 are completely the same.
Control group 2:
The biological feedstuff of this control group is raw material without using bean powder, and raw material comprises the following components in parts by weight: 45 parts
Whey powder, 18 parts of indocalamus leaf, 13 parts of safflower, 1 part of enzyme preparation and 2 parts of prebiotic bacterium source organic selenium solution；Wherein, bean powder
It is made of soybean powder, black bean powder and broad bean powder according to mass ratio for 8:2:1；Enzyme preparation by 150U/mg compound protease and
The carboxypeptidase of 200U/mg is 1:3 composition according to mass ratio, and other embodiment and embodiment 1 are completely the same.
Control group 3:
The biological feedstuff of this control group is raw material without using indocalamus leaf, safflower, and raw material comprises the following components in parts by weight:
45 parts of whey powder, 36 parts of bean powder, 1 part of enzyme preparation and 2 parts of prebiotic bacterium source organic selenium solution；Wherein, bean powder is by soya bean
Powder, black bean powder and broad bean powder are 8:2:1 composition according to mass ratio；Enzyme preparation by 150U/mg compound protease and 200U/mg
Carboxypeptidase is 1:3 composition according to mass ratio, and other embodiment and embodiment 1 are completely the same.
Control group 4:
Compound protease is used only in the biological feedstuff enzyme preparation of this control group, and raw material is by following parts by weight at grouping
At: 45 parts of whey powder, 36 parts of bean powder, 18 parts of indocalamus leaf, 13 parts of safflower, 1 part of enzyme preparation and 2 parts of prebiotic bacterium source have
Machine selenium solution；Wherein, bean powder is made of soybean powder, black bean powder and broad bean powder according to mass ratio for 8:2:1；Enzyme preparation is 150U/
The compound protease of mg is 1:3 composition according to mass ratio, and other embodiment and embodiment 1 are completely the same.
Control group 5:
The prebiotic bacterium source organic selenium solution of the biological feedstuff of this control group does not add the concentrated liquid of tradition Chinese medicine during the cultivation process, it may be assumed that
It is 2.2 × 10 by living bacteria count5The lactic acid bacteria of cfu/mg accesses culture medium prescription according to 1% inoculum concentration are as follows: the horse of 10g/L
Bell potato powder, the whey powder of 5g/L, 3g/L algal polysaccharides, the gumbo polysaccharide of 2g/L, the magnesium sulfate of 1g/L, 1g/L ammonium chloride, 1g/
In the potassium hydrogen phosphate of L, the fluid nutrient medium of the sodium selenite of 1g/L, wherein the pH value of culture medium is 6.9；It is 35 DEG C in temperature
Lower Anaerobic culturel 70h obtains prebiotic bacterium source organic selenium solution；Other embodiment and embodiment 1 are completely the same.
Control group 6:
Whey powder, enzyme preparation and probiotics is used only (without the culture containing the concentrated liquid of tradition Chinese medicine in the biological feedstuff of this control group
Liquid culture, only lactic acid bacteria) it is raw material, raw material comprises the following components in parts by weight: 45 parts of whey powder, 1 part of enzyme system
Agent and 2 parts of living bacteria count are 2.2 × 105The lactic acid bacteria of cfu/mg；Other embodiment and embodiment 1 are completely the same.
Test experiments 1: the determination of bioactive constituent of biological feedstuff:
Soluble-carbohydrate, total amino acid, flavones, dimension life in testing example 1-3 and control group 1-7 biological feedstuff
Plain E, lactic acid bacteria living bacteria count and Se content, are specifically shown in Table 1:
Table 1
As seen from the above table, the soluble-carbohydrate of embodiment 1-3 and control group 5, control group 3 quite, compare control group
1-2, control group 4 and control group 6 are high；Illustrate that the solvable of feed can be improved in the whey powder, bean powder, carboxypeptidase of the application feed formula
Property carbohydrate content；The total amino acid content and control group 3 of embodiment 1-3 is suitable, than control group 1-2 and control group 4-6
It is all high, illustrate to add Chinese medicine liquid that feed can be improved when the whey powder, bean powder, carboxypeptidase, probiotic's culture of the application feed formula total
Amino acid content；The flavones content and control group 4 of embodiment 1-3 is quite, all higher than control group 1-5 and control group 5-6, illustrates this
Apply for that adding Chinese medicine liquid that the flavones in feed can be improved when whey powder, the bean powder, traditional Chinese medicine ingredients, probiotic's culture of feed formula contains
Amount；The content of vitamin E and control group 4 of embodiment 1-3 is quite, all higher than control group 1-5 and control group 5-6, illustrates the application
Add Chinese medicine liquid that the content of vitamin E in feed can be improved when the whey powder of feed formula, bean powder, traditional Chinese medicine ingredients, probiotic's culture；
The viable count of lactobacillus and control group 2 of embodiment 1-3 is quite, all higher than control group 1, control group 3-6, illustrates that the application feed is matched
Side whey powder, traditional Chinese medicine ingredients, carboxypeptidase, probiotic's culture when add Chinese medicine liquid that the viable count of lactobacillus in feed can be improved；It is real
The Se content and control group 3 for applying a 1-3 quite, than control group 1-2 and control group 4-6 high, illustrate the application feed formula
Add Chinese medicine liquid that can effectively improve the Se content in feed when whey powder, bean powder, carboxypeptidase, probiotic's culture.
Testing experiment 2: stomach trouble zoopery:
1, place: Guangxi University's Animal Lab. is tested
2, acetic acid burning property GU experimental model is established:
The small taboo water of Rat Fast for 24 hours after, 3% yellow Jackets 1mL/kg intraperitoneal injection of anesthesia, sterile working, just from xiphoid-process
In down along ventrimeson cut about 2cm opening, gently pull out body of stomach, by from the glass-tube of diameter 5mm, long 30mm perpendicular from being put in paries anterior gastricus sinus
Body boundary serous coat and on, 100% glacial acetic acid is added into pipe O.1mL, contact lmin after blotted with cotton swab, normal saline flushing
Afterwards, stomach is gently sent back to, and is wrapped up with omentum majus, successively stitched Jie's peritonaeum, abdominal muscle and skin, sterilize again, it is postoperative normal
Raising.
Animal packet and nursing:
Animal packet and administration at mould rat 90 are only randomly divided into 9 groups,
1 group of model: with the feed of embodiment 1 and water according to mass ratio be 1:5 be mixedly configured into after suspension to rat into
Row stomach-filling, each 20g, 3 times a day；
2 groups of model: with the feed of control group 1 and water according to mass ratio be 1:5 be mixedly configured into after suspension to rat into
Row stomach-filling, each 20g, 3 times a day；
3 groups of model: with the feed of control group 2 and water according to mass ratio be 1:5 be mixedly configured into after suspension to rat into
Row stomach-filling, each 20g, 3 times a day；
4 groups of model: with the feed of control group 3 and water according to mass ratio be 1:5 be mixedly configured into after suspension to rat into
Row stomach-filling, each 20g, 3 times a day；
5 groups of model: with the feed of control group 4 and water according to mass ratio be 1:5 be mixedly configured into after suspension to rat into
Row stomach-filling, each 20g, 3 times a day；
6 groups of model: with the feed of control group 5 and water according to mass ratio be 1:5 be mixedly configured into after suspension to rat into
Row stomach-filling, each 20g, 3 times a day；
7 groups of model: with the feed of control group 6 and water according to mass ratio be 1:5 be mixedly configured into after suspension to rat into
Row stomach-filling, each 20g, 3 times a day；
Control group: carrying out stomach-filling to rat with physiological saline, each 20g, 3 times a day.
Indices detection is carried out after 20 days, Testing index is as follows:
1, rat is killed, the stomach of rat is cut off, goes out the maximum major diameter of ulcer with vernier caliper measurement and perpendicular to major diameter
Maximum minor axis, the product of the two is as ulcer area index；
2, the measurement neutralisation of gastric acid and pepsin measurement total acidity gastric juice (p H value), improvement Mett method measure stomach egg
White enzymatic activity, measurement result are shown in Table 2:
Table 2
| Group | Ulcer area (mm2) | PH value | Pepsin activity (KU/L) |
| 1 group of model | 0.76 | 3.4 | 361.25 |
| 2 groups of model | 0.84 | 3.2 | 362.45 |
| 3 groups of model | 0.86 | 3.4 | 357.48 |
| 4 groups of model | 3.54 | 5.1 | 431.54 |
| 5 groups of model | 0.86 | 3.6 | 360.47 |
| 6 groups of model | 4.68 | 5.3 | 429.58 |
| 7 groups of model | 6.34 | 5.4 | 440.15 |
| Control group | 6.48 | 5.4 | 437.54 |
As seen from the above table, the ulcer area, pH value of model 1-3, pepsin activity are significantly lower than model group 4, model group
6-7 and control group；Illustrate that the Feed Energy of the application effectively prevents the formation of ulcer area, protects stomach；It is risen in feed formula
To prevention and treatment ulcer area formed ingredient be indocalamus leaf, safflower and using the concentrated liquid of tradition Chinese medicine to probiotics carry out culture promote it is prebiotic
Bacterium growth and breeding.
Influence of the testing experiment 3:(feed formula to water cow mammary gland function)
The water cow of 80 nursing periods is divided into 8 groups, every group feeds water cow (test group 1-7) by following feed formula
And control group, carry out the raising of 30d by a definite date, before feeding 0d, 10d, 20d, between 30d 9:00-10:00 in morning
The content of somatic number (SCC) in water cow milk 100ml test water cow milk is taken, 30d tests the Se content in water cow milk,
Record the results are shown in Table 3:
Experiment periods feed formula:
Test group 1: water cow is fed with the feed of embodiment 1；
Test group 2: water cow is fed with the feed of control group 1；
Test group 3: water cow is fed with the feed of control group 2；
Test group 4: water cow is fed with the feed of control group 3；
Test group 5: water cow is fed with the feed of control group 4；
Test group 6: water cow is fed with the feed of control group 5；
Test group 7: water cow is fed with the feed of control group 6；
Control group: feeder greens feeds water cow；
Table 3
As seen from the above table, the content of somatic number (SCC) is almost the same in test group 1-7 and control group 0d buffalo milk,
After adding the application feed addictive nursing 10d, 20d, 30d, somatic number (SCC) in test group 1-7 buffalo milk
Content gradually decreases, and the biggest drop is test group 1, and the test group 2-7 range of decrease is almost the same, and control group is basically unchanged；Explanation
The feed formula collaboration of the application uses the content that can obviously reduce somatic number in buffalo milk (SCC)；To further prove
, the mammary gland function of water cow can be improved in the feed formula of the application, and has synergistic effect between each ingredient；The selenium of test group 1 contains
It measures higher than test group 2-7 and control group, wherein the Se content of test group 4 is than test group 2-3, test group 5-7 and control group；By
This is it is found that the indocalamus leaf of the application feed formula, safflower are smaller on the influence of dairy buffalo Se content.
The application is not limited to the protection to dairy buffalo mammary gland function, applies also for other mammals such as pig, ox, sheep.
In conclusion the feed of the application soluble-carbohydrate with higher, total amino acid, flavones, vitamin
E, lactic acid bacteria living bacteria count and Se content can effectively protect the mammary gland of animal stomach and mammal using the feed of the application
Function improves mammal to the conversion ratio of selenium to reach, improves the purpose of Se content in dairy products.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the patents of the present invention.
Claims (7)
1. a kind of lactalbumin Se-enriched bio feed, which is characterized in that the feed is made of following parts by weight ingredient: 45 parts-
57 parts of whey powder, 36 parts -43 parts of bean powder, 18 parts -27 parts of indocalamus leaf, 13 parts -24 parts of safflower, 1 part -3 parts of enzyme preparation
With 2 parts -5 parts of prebiotic bacterium source organic selenium solution.
2. a kind of lactalbumin Se-enriched bio feed according to claim 1, which is characterized in that the bean powder is by soya bean
Powder, black bean powder and broad bean powder are 8-10:2-4:1-3 composition according to mass ratio.
3. a kind of lactalbumin Se-enriched bio feed according to claim 1, which is characterized in that the enzyme preparation by
The carboxypeptidase of the compound protease of 150U/mg-200U/mg and 200U/mg-250U/mg are that 1:3-4 is formed according to mass ratio.
4. a kind of lactalbumin Se-enriched bio feed according to claim 1, which is characterized in that the prebiotic bacterium source is organic
Selenium solution is lactic acid bacterium source organic selenium solution, and it is 2.2 × 10 by living bacteria count that preparation method, which is,5-5.2×105cfu/mg
Lactic acid bacteria according to 1%-3% inoculum concentration access the fluid nutrient medium containing the concentrated liquid of tradition Chinese medicine, temperature be 35-38 DEG C at anaerobism
Cultivate 70h-72h to obtain the final product.
5. a kind of lactalbumin Se-enriched bio feed according to claim 4, which is characterized in that described to contain the concentrated liquid of tradition Chinese medicine
Fluid nutrient medium are as follows: the Radix Isatidis of 1g/L-3g/L, the pueraria lobata of 1g/L-3g/L, the aloe of 1g/L-3g/L, 10g/L-15g/L
Dehydrated potato powder, the whey powder of 5g/L-10g/L, 3g/L-5g/L algal polysaccharides, 2g/L-4g/L gumbo polysaccharide, 1g/L-3g/L
Magnesium sulfate, the ammonium chloride of 1g/L-3g/L, the potassium hydrogen phosphate of 1g/L-3g/L, 1g/L sodium selenite.
6. a kind of method for preparing the lactalbumin Se-enriched bio feed as described in claim 1-5 any one, which is characterized in that
The method are as follows: weigh each raw material by weight, spray 35-37 DEG C to mixture after whey powder, bean powder and enzyme preparation are mixed
Thermostatted water, kneading is agglomerating to be digested for 24 hours under conditions of 37 DEG C, and it is molten then to add indocalamus leaf, safflower and prebiotic bacterium source organic selenium
The thermostatted water that liquid is further continued for 35-37 DEG C of sprinkling stirs the mixture for into paste, then ferment at constant temperature 5- under conditions of 35-40 DEG C
7 days to obtain the final product.
7. -5 any lactalbumin Se-enriched bio feeds are in protection mammal galactophore function according to claim 1
Purposes.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109536422A (en) * | 2019-01-10 | 2019-03-29 | 广西大学 | A kind of aerobic high-density cultivation method of lactic acid bacteria |
| CN110200151A (en) * | 2019-06-06 | 2019-09-06 | 西藏自治区农牧科学院畜牧兽医研究所 | A kind of yak feed addictive and preparation method thereof that yak survival rate can be improved |
| CN110583859A (en) * | 2019-09-23 | 2019-12-20 | 广西黑硒牛农业科技有限公司 | Organic selenium ecological amino acid nutrient and preparation method thereof |
| CN112481341A (en) * | 2020-12-07 | 2021-03-12 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method and application of selenium absorption enhancing active peptide |
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| CN103751220A (en) * | 2011-10-20 | 2014-04-30 | 朱琴 | Selenium-rich lactobacillus preparation and production method thereof |
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| CN103751220A (en) * | 2011-10-20 | 2014-04-30 | 朱琴 | Selenium-rich lactobacillus preparation and production method thereof |
| CN104431427A (en) * | 2014-11-12 | 2015-03-25 | 张华琦 | Premix for piglets of special wild boars and mixed feed thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109536422A (en) * | 2019-01-10 | 2019-03-29 | 广西大学 | A kind of aerobic high-density cultivation method of lactic acid bacteria |
| CN109536422B (en) * | 2019-01-10 | 2022-02-01 | 广西大学 | Aerobic high-density culture method of lactic acid bacteria |
| CN110200151A (en) * | 2019-06-06 | 2019-09-06 | 西藏自治区农牧科学院畜牧兽医研究所 | A kind of yak feed addictive and preparation method thereof that yak survival rate can be improved |
| CN110583859A (en) * | 2019-09-23 | 2019-12-20 | 广西黑硒牛农业科技有限公司 | Organic selenium ecological amino acid nutrient and preparation method thereof |
| CN112481341A (en) * | 2020-12-07 | 2021-03-12 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method and application of selenium absorption enhancing active peptide |
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Application publication date: 20190308 |