CN109430272A - A kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides - Google Patents
A kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides Download PDFInfo
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- CN109430272A CN109430272A CN201811467914.3A CN201811467914A CN109430272A CN 109430272 A CN109430272 A CN 109430272A CN 201811467914 A CN201811467914 A CN 201811467914A CN 109430272 A CN109430272 A CN 109430272A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention discloses a kind of plant resistance to environment stress inducers containing new fine jade oligosaccharides, belong to technical field of plant nutrition.The plant resistance to environment stress inducer raw material includes new fine jade oligosaccharides, the sugared bacillus of cold solution and tamarind seed polysaccharide, wherein new fine jade oligosaccharides and tamarind seed polysaccharide mass ratio are as follows: new 20~40 parts, 5~15 parts of tamarind seed polysaccharide of oligosaccharides of fine jade.The present invention is using new fine jade oligosaccharides and tamarind seed polysaccharide as main component, it is aided with the sugared bacillus of cold solution and prepares efficient plant resistance to environment stress inducer, the action and efficacy of product of the present invention is improved by the obvious synergy between raw material, it can be obviously improved crop crop anti-adversity under the environmental stress conditions such as low temperature, arid, saline and alkaline, promote the Increase production ability under environmental stress conditions.Product of the present invention all has very significant effect to the degeneration-resistant significant effect of the environmental stress conditions such as saline and alkaline, arid, low temperature, to the fruits and vegetables such as the field crops such as wheat, rice and cucumber, tomato.
Description
Technical field
The present invention relates to a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides more particularly to it is a kind of using new fine jade oligosaccharides,
The raw materials such as the sugared bacillus of cold solution, tamarind seed polysaccharide prepare plant resistance to environment stress inducer, belong to technical field of plant nutrition.
Background technique
It is inverse to resist can be generated the series reactions such as phytoalexin, antibacterial protein by extraneous environment stress for plant
The infringement in border.If plant responds not in time or is suppressed to the defence of adverse circumstance, the growth of plant will receive influence.It utilizes
Inducer stimulates plant to generate degeneration-resistant substance, so that enhancing plant is a kind of new plant protection to the resistivity of extraneous adverse circumstance
Approach.It is induced by inducer, the anti-adversity ability of plant can be enhanced, reduced environment stress and damaged caused by plant, repaired
Part physiological function.Oligosaccharides is to study more a kind of plant resistance inducer at present, thin from the discovery plant such as Ebel in 1976
After oligosaccharides fragment on cell wall can induce soybean generation glyceollin, oligosaccharides is increasingly closed as plant resistance inducer
Note.
Oligosaccharides with induction stress resistance of plant mainly has plant origin oligosaccharides (such as oligogalacturonans), fungi
Source oligosaccharides (such as β-Portugal's oligosaccharides), animal origin oligosaccharides (such as chitosan oligosaccharide) and seaweed source oligosaccharides (such as brown alga oligose, carragheen
Oligosaccharides etc.).With the development of research work, oligosaccharides has been widely accepted as the concept of plant resistance inducer.And ocean is few
Sugar is as important component in oligosaccharides, compared with the oligosaccharides in other sources, has the characteristics that abundance, acquisition are simple,
Have preferable development and application potentiality on plant resistance inducer.
Agaropectin oligose is that origin is degraded derived from the agar-agar of marine red alga (such as asparagus, agar).It is by fine jade
Disaccharides is formed by connecting for recurring unit, including two series of new fine jade oligosaccharides and fine jade oligosaccharides.Fine jade oligosaccharides is with 3,6- inner ether-α-L- gala
Saccharide residue is reducing end under neutral, and new fine jade oligosaccharides is using β-D- galactose residue as reducing end under neutral.Agaropectin oligose is by acid or α-fine jade
Glue cracks α -1 of enzymatic lysis agarose, and 3 glycosidic bonds generate, and new fine jade oligosaccharides is by β-agar-agar cracking enzymatic lysis agarose β -
What Isosorbide-5-Nitrae glycosidic bond obtained.Agaropectin oligose can discharge H with inducing plant activity oxidative burst2O2, promote plant physiology reaction, from
Environment stress infringement.Chen Haimin etc. handles Phaseolus Leaves with the agaropectin oligose of 100 mg/L, and discovery agaropectin oligose can promote Kidney bean
Blade lower epidermis guard cell discharges H2O2Gentle bore closure, and LOX activity can be significantly improved, while improving 2,3- diacetyl, 1-
Octene -3- alcohol etc. has the content of the volatile materials of bacteriostasis, and Kidney bean is made to generate certain resistance.Patent
201710068358.1 disclose it is a kind of improve crop salt-resistance marine oligosaccharide biological agent, raw material include chitin oligosaccharide,
Asparagus agaropectin oligose, bacillusmusilaginosiengineering, calcium chloride and water, the marine oligosaccharide biological agent are peppery under salt stress for promoting
Green pepper seed is sprouted, and the germination rate of pepper seed can be significantly improved, and promotes Seedlings development, enhances pepper seedling to salt stress
Resistivity is equally applicable to promote the growth of other crop seeds such as cucumber, rice sprouting and seedling.
Although current agaropectin oligose has been achieved for some achievements in the application of stress resistance of plant, in the prior art ocean
Application of the oligosaccharides in stress resistance of plant preparation is still based on brown alga oligose, about agaropectin oligose using relatively fewer, especially
Have no the report that new fine jade oligosaccharides is used for plant inducer, and agaropectin oligose plant resistance to environment stress inducer product efficacy and broad spectrum activity need
It further to be promoted, break the bottleneck for restricting agaropectin oligose and applying in plant resistance to environment stress inducer with this.
Summary of the invention
In order to overcome the above-mentioned deficiencies of the prior art, meet the nutritional need of plant stress-resistance volume increase, the present invention provides one kind
Plant resistance to environment stress inducer containing new fine jade oligosaccharides, integrates plant stress-resistance inductive technology and marine oligosaccharide functional active components compound skill
Art, developing has effects that significant degeneration-resistant, growth promotion, volume increase, the plant resistance to environment stress inducer for improving quality.
The present invention is achieved through the following technical solutions above-mentioned technical effect:
A kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides, the plant resistance to environment stress inducer raw material include new fine jade oligosaccharides, cold solution
Sugared bacillus and tamarind seed polysaccharide, wherein new fine jade oligosaccharides and tamarind seed polysaccharide mass ratio are as follows: new 20~40 parts of fine jade oligosaccharides, Luo Wang
5~15 parts of sub- polysaccharide.
Optimization, a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides, the plant resistance to environment stress inducer raw material includes new
The sugared bacillus of fine jade oligosaccharides, cold solution and tamarind seed polysaccharide, wherein new fine jade oligosaccharides and tamarind seed polysaccharide mass ratio are as follows: new fine jade oligosaccharides 25
~35 parts, 8~13 parts of tamarind seed polysaccharide.
Optimal, a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides, the plant resistance to environment stress inducer raw material includes new
The sugared bacillus of fine jade oligosaccharides, cold solution and tamarind seed polysaccharide, wherein new fine jade oligosaccharides and tamarind seed polysaccharide mass ratio are as follows: new fine jade oligosaccharides 32
Part, 12 parts of tamarind seed polysaccharide.
The new fine jade oligosaccharides is the even number oligosaccharide after the agaropectin oligose enzymatic hydrolysis that the degree of polymerization is 2-20.
Preferably, the new fine jade oligosaccharides is the new fine jade oligosaccharides of asparagus.
The present invention provides a kind of plant resistance to environment stress inducer of new fine jade oligosaccharides, and the plant resistance to environment stress inducer is as follows
Preparation:
(1) the sugared fermentation of bacillus liquid preparation of cold solution
Nitrogen-free solid medium is prepared, the sugared Bacillus strain of cold solution is seeded to nitrogen-free solid medium and carries out scribing line culture,
It the activated sugared bacillus of cold solution will be forwarded in nitrogen-free fluid nutrient medium on nitrogen-free solid medium, culture obtains level-one
Primary seed solution is seeded in seed fermentation tank by seed liquor, and culture obtains secondary seed solution；Seed liquor is seeded to fermentation again
It ferments in culture medium, the sugared fermentation of bacillus liquid of cold solution is made；
(2) preparation contains the plant resistance to environment stress inducer of new fine jade oligosaccharides
The sugared fermentation of bacillus liquid of cold solution is added to after new fine jade oligosaccharides and tamarind seed polysaccharide are mixed in proportion, and it is few to control new fine jade
Concentration >=0.2% of sugar and tamarind seed polysaccharide, is uniformly mixed, and both obtains the plant resistance to environment stress inducer containing new fine jade oligosaccharides.
The sugared bacillus living bacteria count >=200,000,000/mL of cold solution in the above-mentioned sugared fermentation of bacillus liquid of cold solution.
The group of above-mentioned solid nitrogen-free agar becomes sucrose 10g/L, potassium dihydrogen phosphate 0.6g/L, magnesium sulfate 0.4g/L, chlorine
Change sodium 0.15g/L, calcium carbonate 0.5g/L, agar 20g/L；PH value is 7.0 before sterilizing；The condition of the activation culture is 30 DEG C
Under the conditions of cultivate 48h.
The group of above-mentioned seed culture medium becomes sucrose 10g/L, potassium dihydrogen phosphate 0.6g/L, magnesium sulfate 0.4g/L, sodium chloride
0.15g/L, calcium carbonate 0.5g/L；PH value is 7.0 before sterilizing；The condition of the first order seed culture can be in 30 DEG C, 150rpm
Under the conditions of cultivate for 24 hours；The condition of the secondary seed culture can be in fermentor, 30 DEG C, keeps tank revolving speed 120-150rpm
Under the conditions of cultivate for 24 hours.
The group of above-mentioned fermentation medium becomes yeast extract 1.5g/L, starch 5.0g/L, bean cake powder 5.0g/L, magnesium sulfate
1.2g/L, potassium dihydrogen phosphate 1.5g/L, calcium carbonate 5.0g/L, pH is 7.0 before sterilizing；The seed liquor is seeded to fermentation tank culture
The inoculum concentration fermented in base is the 10% of volume ratio；The fermentation condition can are as follows: revolving speed is controlled in 180-250rpm, dissolved oxygen control
System is 20%, and at 30 DEG C, tank pressure is maintained at 0.05-0.06Mpa for tank temperature control, and pH value control is 7.2, fermentation time 48-72h.
The present invention provides a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides, can be used for improving field crop, vegetables,
The degeneration-resistant growth promotion of melon and fruit.
The present invention provides a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides, compared with prior art, has following aobvious
Work advantage:
(1) present invention is aided with the sugared efficient plant of bacillus preparation of cold solution using new fine jade oligosaccharides and tamarind seed polysaccharide as main component
Object resistance inductors improve the action and efficacy of product of the present invention by the obvious synergy between raw material, can significantly mention
Crop crop anti-adversity under the environmental stress conditions such as low temperature, arid, saline and alkaline is risen, the volume increase energy under environmental stress conditions is promoted
Power；
(2) present invention is containing new fine jade oligosaccharides, tamarind seed polysaccharide, the sugared bacillus isoreactivity ingredient of cold solution, wherein the new fine jade used
Oligosaccharides is easy to easily enter in cytoplasm and nucleus through bacterial membrane, promotes new fine jade oligosaccharides since relative molecular mass is small
Plant resistance to environment stress inducing action, by increase plant in enzyme content and soluble sugar content alleviate environment-stress to vegetational zone
The damage come, the experimental results showed that the degeneration-resistant effect of product can be significantly reduced if raw material is using agaropectin oligose；Tamarind seed polysaccharide
Addition can dramatically increase the degeneration-resistant effect of product of the present invention, planted in current existing open file there is no tamarind seed polysaccharide
The correlative study of object resistance application；
(3) test result shows product of the present invention to the degeneration-resistant significant effect of the environmental stress conditions such as saline and alkaline, arid, low temperature, to small
The fruits and vegetables such as the field crops such as wheat, rice and cucumber, tomato all have very significant effect.
Specific embodiment
Embodiment 1
A kind of plant resistance to environment stress inducer of new fine jade oligosaccharides, the plant resistance to environment stress inducer are made by the steps:
(1) the sugared fermentation of bacillus liquid preparation of cold solution
Nitrogen-free solid medium is prepared, the sugared Bacillus strain of cold solution is seeded to nitrogen-free solid medium and carries out scribing line culture,
It the activated sugared bacillus of cold solution will be forwarded in nitrogen-free fluid nutrient medium on nitrogen-free solid medium, culture obtains level-one
Primary seed solution is seeded in seed fermentation tank by seed liquor, and culture obtains secondary seed solution；Seed liquor is seeded to fermentation again
It ferments in culture medium, the sugared fermentation of bacillus liquid of cold solution is made；The group of the solid nitrogen-free agar becomes sucrose 10g/L, phosphorus
Acid dihydride potassium 0.6g/L, magnesium sulfate 0.4g/L, sodium chloride 0.15g/L, calcium carbonate 0.5g/L, agar 20g/L；PH value is before sterilizing
7.0；The condition of the activation culture is to cultivate 48h under the conditions of 30 DEG C；The group of the seed culture medium become sucrose 10g/L,
Potassium dihydrogen phosphate 0.6g/L, magnesium sulfate 0.4g/L, sodium chloride 0.15g/L, calcium carbonate 0.5g/L；PH value is 7.0 before sterilizing；It is described
The condition of first order seed culture can be cultivated for 24 hours under the conditions of 150rpm in 30 DEG C；The condition of the secondary seed culture can be in
In fermentor, 30 DEG C, keep cultivating for 24 hours under the conditions of tank revolving speed 120-150rpm；The group of the fermentation medium becomes yeast extract
1.5g/L, starch 5.0g/L, bean cake powder 5.0g/L, magnesium sulfate 1.2g/L, potassium dihydrogen phosphate 1.5g/L, calcium carbonate 5.0g/L, go out
PH is 7.0 before bacterium；It is the 10% of volume ratio that the seed liquor, which is seeded to the inoculum concentration fermented in fermentation tank culture medium,；The fermentation
Condition can are as follows: revolving speed control is in 180-250rpm, and dissolved oxygen control is 20%, and tank temperature is controlled at 30 DEG C, and tank pressure is maintained at 0.05-
0.06Mpa, pH value control is 7.2, fermentation time 48-72h；The sugared bacillus of cold solution is effective in the sugared fermentation of bacillus liquid of cold solution
Viable count >=200,000,000/mL；
(2) preparation contains the plant resistance to environment stress inducer of new fine jade oligosaccharides
The sugared bud of cold solution is added to after new fine jade oligosaccharides and tamarind seed polysaccharide are mixed in 12 parts of 32 parts of glue oligosaccharides, tamarind seed polysaccharide ratios
Spore bacillus fermentation liquid, and concentration >=0.2% of new fine jade oligosaccharides and tamarind seed polysaccharide is controlled, it is uniformly mixed, both obtains and contain new fine jade
The plant resistance to environment stress inducer of oligosaccharides；The new fine jade oligosaccharides is that the even number after the asparagus agaropectin oligose enzymatic hydrolysis that the degree of polymerization is 2-20 is few
Sugar.
Embodiment 2
A kind of plant resistance to environment stress inducer of new fine jade oligosaccharides, the plant resistance to environment stress inducer are made by the steps:
(1) the sugared fermentation of bacillus liquid preparation of cold solution
Nitrogen-free solid medium is prepared, the sugared Bacillus strain of cold solution is seeded to nitrogen-free solid medium and carries out scribing line culture,
It the activated sugared bacillus of cold solution will be forwarded in nitrogen-free fluid nutrient medium on nitrogen-free solid medium, culture obtains level-one
Primary seed solution is seeded in seed fermentation tank by seed liquor, and culture obtains secondary seed solution；Seed liquor is seeded to fermentation again
It ferments in culture medium, the sugared fermentation of bacillus liquid of cold solution is made；The group of the solid nitrogen-free agar becomes sucrose 10g/L, phosphorus
Acid dihydride potassium 0.6g/L, magnesium sulfate 0.4g/L, sodium chloride 0.15g/L, calcium carbonate 0.5g/L, agar 20g/L；PH value is before sterilizing
7.0；The condition of the activation culture is to cultivate 48h under the conditions of 30 DEG C；The group of the seed culture medium become sucrose 10g/L,
Potassium dihydrogen phosphate 0.6g/L, magnesium sulfate 0.4g/L, sodium chloride 0.15g/L, calcium carbonate 0.5g/L；PH value is 7.0 before sterilizing；It is described
The condition of first order seed culture can be cultivated for 24 hours under the conditions of 150rpm in 30 DEG C；The condition of the secondary seed culture can be in
In fermentor, 30 DEG C, keep cultivating for 24 hours under the conditions of tank revolving speed 120-150rpm；The group of the fermentation medium becomes yeast extract
1.5g/L, starch 5.0g/L, bean cake powder 5.0g/L, magnesium sulfate 1.2g/L, potassium dihydrogen phosphate 1.5g/L, calcium carbonate 5.0g/L, go out
PH is 7.0 before bacterium；It is the 10% of volume ratio that the seed liquor, which is seeded to the inoculum concentration fermented in fermentation tank culture medium,；The fermentation
Condition can are as follows: revolving speed control is in 180-250rpm, and dissolved oxygen control is 20%, and tank temperature is controlled at 30 DEG C, and tank pressure is maintained at 0.05-
0.06Mpa, pH value control is 7.2, fermentation time 48-72h；The sugared bacillus of cold solution is effective in the sugared fermentation of bacillus liquid of cold solution
Viable count >=200,000,000/mL；
(2) preparation contains the plant resistance to environment stress inducer of new fine jade oligosaccharides
The sugared bud of cold solution is added to after new fine jade oligosaccharides and tamarind seed polysaccharide are mixed in 8 parts of 35 parts of glue oligosaccharides, tamarind seed polysaccharide ratios
Spore bacillus fermentation liquid, and concentration >=0.2% of new fine jade oligosaccharides and tamarind seed polysaccharide is controlled, it is uniformly mixed, both obtains and contain new fine jade
The plant resistance to environment stress inducer of oligosaccharides；The new fine jade oligosaccharides is that the even number after the asparagus agaropectin oligose enzymatic hydrolysis that the degree of polymerization is 2-20 is few
Sugar.
Embodiment 3
A kind of plant resistance to environment stress inducer of new fine jade oligosaccharides, the plant resistance to environment stress inducer are made by the steps:
(1) the sugared fermentation of bacillus liquid preparation of cold solution
Nitrogen-free solid medium is prepared, the sugared Bacillus strain of cold solution is seeded to nitrogen-free solid medium and carries out scribing line culture,
It the activated sugared bacillus of cold solution will be forwarded in nitrogen-free fluid nutrient medium on nitrogen-free solid medium, culture obtains level-one
Primary seed solution is seeded in seed fermentation tank by seed liquor, and culture obtains secondary seed solution；Seed liquor is seeded to fermentation again
It ferments in culture medium, the sugared fermentation of bacillus liquid of cold solution is made；The group of the solid nitrogen-free agar becomes sucrose 10g/L, phosphorus
Acid dihydride potassium 0.6g/L, magnesium sulfate 0.4g/L, sodium chloride 0.15g/L, calcium carbonate 0.5g/L, agar 20g/L；PH value is before sterilizing
7.0；The condition of the activation culture is to cultivate 48h under the conditions of 30 DEG C；The group of the seed culture medium become sucrose 10g/L,
Potassium dihydrogen phosphate 0.6g/L, magnesium sulfate 0.4g/L, sodium chloride 0.15g/L, calcium carbonate 0.5g/L；PH value is 7.0 before sterilizing；It is described
The condition of first order seed culture can be cultivated for 24 hours under the conditions of 150rpm in 30 DEG C；The condition of the secondary seed culture can be in
In fermentor, 30 DEG C, keep cultivating for 24 hours under the conditions of tank revolving speed 120-150rpm；The group of the fermentation medium becomes yeast extract
1.5g/L, starch 5.0g/L, bean cake powder 5.0g/L, magnesium sulfate 1.2g/L, potassium dihydrogen phosphate 1.5g/L, calcium carbonate 5.0g/L, go out
PH is 7.0 before bacterium；It is the 10% of volume ratio that the seed liquor, which is seeded to the inoculum concentration fermented in fermentation tank culture medium,；The fermentation
Condition can are as follows: revolving speed control is in 180-250rpm, and dissolved oxygen control is 20%, and tank temperature is controlled at 30 DEG C, and tank pressure is maintained at 0.05-
0.06Mpa, pH value control is 7.2, fermentation time 48-72h；The sugared bacillus of cold solution is effective in the sugared fermentation of bacillus liquid of cold solution
Viable count >=200,000,000/mL；
(2) preparation contains the plant resistance to environment stress inducer of new fine jade oligosaccharides
The sugared bud of cold solution is added to after new fine jade oligosaccharides and tamarind seed polysaccharide are mixed in 13 parts of 25 parts of glue oligosaccharides, tamarind seed polysaccharide ratios
Spore bacillus fermentation liquid, and concentration >=0.2% of new fine jade oligosaccharides and tamarind seed polysaccharide is controlled, it is uniformly mixed, both obtains and contain new fine jade
The plant resistance to environment stress inducer of oligosaccharides；The new fine jade oligosaccharides is that the even number after the asparagus agaropectin oligose enzymatic hydrolysis that the degree of polymerization is 2-20 is few
Sugar.
Embodiment 4
A kind of plant resistance to environment stress inducer tomato Drought resistant experiment containing new fine jade oligosaccharides of the present invention:
1) material to be tested
Rays of sunlight tomato (late-maturing one dimensional infinite wall) is selected in test；
2) experimental method
Test method is the same as Sun Jin etc. " influence and mechanism study of the marine algae extract to the drought resistance of tomato "；It is divided into 6 test groups,
Respectively embodiment 1-3 test group, blank control group, comparative examples 1-2:
Blank control group: foliage-spray pure water；
Comparative examples 1: the tamarind seed polysaccharide in embodiment 1 is substituted with new fine jade oligosaccharides, remaining is the same as embodiment 1；
Comparative examples 2: the new fine jade oligosaccharides in embodiment 1 is substituted with tamarind seed polysaccharide, remaining is the same as embodiment 1；
3) measurement item
Testing index includes rate of water loss, soluble sugar, SOD enzyme activity, drought resistance coefficient, yield etc., and measuring method is " extra large with brocade equal
Influence and mechanism study of the algae extract to the drought resistance of tomato "；
4) experimental result
Statistical result is shown in Table 1:
A kind of plant resistance to environment stress inducer effect test containing new fine jade oligosaccharides of table 1
It can be seen that by 1 result of table, the resistance inductors provided by the invention containing new fine jade oligosaccharides can significantly improve the anti-of tomato
Non-irrigated ability promotes the growth and production capacity of tomato under drought stress conditions.In addition, experimental result also shows new fine jade oligosaccharides and sieve
Synergistic effect between shop sign in the form of a streamer polysaccharide has been obviously improved the degeneration-resistant effect of product, achieves the technical effect of " 1+1 > 2 ".
The above examples are only used to illustrate the technical scheme of the present invention, rather than is limited；Although referring to aforementioned reality
It applies example to be described in detail to by invention, but for those of ordinary skill in the art, it still can be to aforementioned reality
Technical solution documented by example is applied to modify or equivalent replacement of some of the technical features；And to these modifications
Or replacement, the spirit and scope for claimed technical solution of the invention that it does not separate the essence of the corresponding technical solution.
Claims (10)
1. a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides, the plant resistance to environment stress inducer raw material includes new fine jade oligosaccharides, cold
Sugared bacillus and tamarind seed polysaccharide are solved, wherein new fine jade oligosaccharides and tamarind seed polysaccharide mass ratio are as follows: new 20~40 parts of fine jade oligosaccharides, sieve
5~15 parts of shop sign in the form of a streamer polysaccharide.
2. a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides according to claim 1, it is characterised in that the plant
Resistance inductors raw material includes new fine jade oligosaccharides, the sugared bacillus of cold solution and tamarind seed polysaccharide, wherein new fine jade oligosaccharides and tamarind are more
Sugared mass ratio are as follows: new 25~35 parts, 8~13 parts of tamarind seed polysaccharide of oligosaccharides of fine jade.
3. a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides according to claim 1, it is characterised in that the plant
Resistance inductors raw material includes new fine jade oligosaccharides, the sugared bacillus of cold solution and tamarind seed polysaccharide, wherein new fine jade oligosaccharides and tamarind are more
Sugared mass ratio are as follows: new 32 parts, 12 parts of tamarind seed polysaccharide of oligosaccharides of fine jade.
4. a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides according to claim 1, it is characterised in that the new fine jade
Oligosaccharides is the even number oligosaccharide after the agaropectin oligose enzymatic hydrolysis that the degree of polymerization is 2-20.
5. a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides according to claim 1, it is characterised in that the new fine jade
Oligosaccharides is the new fine jade oligosaccharides of asparagus.
6. a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides according to claim 1, it is characterised in that the plant
Resistance inductors are made by the steps:
The sugared fermentation of bacillus liquid preparation of cold solution
Nitrogen-free solid medium is prepared, the sugared Bacillus strain of cold solution is seeded to nitrogen-free solid medium and carries out scribing line culture,
It the activated sugared bacillus of cold solution will be forwarded in nitrogen-free fluid nutrient medium on nitrogen-free solid medium, culture obtains level-one
Primary seed solution is seeded in seed fermentation tank by seed liquor, and culture obtains secondary seed solution；Seed liquor is seeded to fermentation again
It ferments in culture medium, the sugared fermentation of bacillus liquid of cold solution is made；
(2) preparation contains the plant resistance to environment stress inducer of new fine jade oligosaccharides
The sugared fermentation of bacillus liquid of cold solution is added to after new fine jade oligosaccharides and tamarind seed polysaccharide are mixed in proportion, and it is few to control new fine jade
Concentration >=0.2% of sugar and tamarind seed polysaccharide, is uniformly mixed, and both obtains the plant resistance to environment stress inducer containing new fine jade oligosaccharides.
7. a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides according to claim 6, it is characterised in that the cold solution
The sugared bacillus living bacteria count >=200,000,000/mL of cold solution in sugared fermentation of bacillus liquid.
8. a kind of plant resistance to environment stress inducer containing new fine jade oligosaccharides according to claim 6, it is characterised in that the solid
The group of nitrogen-free agar becomes sucrose 10g/L, potassium dihydrogen phosphate 0.6g/L, magnesium sulfate 0.4g/L, sodium chloride 0.15g/L, carbonic acid
Calcium 0.5g/L, agar 20g/L；PH value is 7.0 before sterilizing；The condition of the activation culture is to cultivate 48h under the conditions of 30 DEG C；Institute
The group for stating seed culture medium becomes sucrose 10g/L, potassium dihydrogen phosphate 0.6g/L, magnesium sulfate 0.4g/L, sodium chloride 0.15g/L, carbon
Sour calcium 0.5g/L；PH value is 7.0 before sterilizing；The condition of the first order seed culture can cultivate under the conditions of 150rpm in 30 DEG C
24h；The condition of the secondary seed culture can be in fermentor, 30 DEG C, keeps cultivating under the conditions of tank revolving speed 120-150rpm
24h；The group of the fermentation medium become yeast extract 1.5g/L, starch 5.0g/L, bean cake powder 5.0g/L, magnesium sulfate 1.2g/L,
Potassium dihydrogen phosphate 1.5g/L, calcium carbonate 5.0g/L, pH is 7.0 before sterilizing；The seed liquor, which is seeded in fermentation tank culture medium, to be sent out
The inoculum concentration of ferment is the 10% of volume ratio；The fermentation condition can are as follows: revolving speed control exists in 180-250rpm, dissolved oxygen control
20%, at 30 DEG C, tank pressure is maintained at 0.05-0.06Mpa for tank temperature control, and pH value control is 7.2, fermentation time 48-72h.
9. by the plant resistance to environment stress inducer containing new fine jade oligosaccharides of the preparation of technique described in claim 1-8.
10. the application of the plant resistance to environment stress inducer described in claim 9 containing new fine jade oligosaccharides.
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