CN109426124A - Pre-amplification off-axis digital holography microscopic system - Google Patents
Pre-amplification off-axis digital holography microscopic system Download PDFInfo
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- CN109426124A CN109426124A CN201710747150.2A CN201710747150A CN109426124A CN 109426124 A CN109426124 A CN 109426124A CN 201710747150 A CN201710747150 A CN 201710747150A CN 109426124 A CN109426124 A CN 109426124A
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- amici prism
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- 238000001093 holography Methods 0.000 title claims abstract description 20
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 15
- 239000004606 Fillers/Extenders Substances 0.000 claims abstract description 10
- 230000010287 polarization Effects 0.000 claims abstract description 10
- 230000000644 propagated effect Effects 0.000 claims abstract description 4
- 230000003321 amplification Effects 0.000 claims description 9
- 238000013139 quantization Methods 0.000 claims description 4
- 230000001678 irradiating effect Effects 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 abstract description 2
- 230000003287 optical effect Effects 0.000 description 11
- 238000000034 method Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 101100117236 Drosophila melanogaster speck gene Proteins 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005094 computer simulation Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/04—Processes or apparatus for producing holograms
- G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
-
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/04—Processes or apparatus for producing holograms
- G03H1/10—Processes or apparatus for producing holograms using modulated reference beam
- G03H1/12—Spatial modulation, e.g. ghost imaging
Abstract
Pre-amplification off-axis digital holography microscopic system proposed by the present invention, it is related to holography field, including laser, the light wave that laser issues reaches polarization splitting prism after the first attenuator and the first half-wave plate, light wave is divided into reference light and object light, reference light enters the first collimator and extender device after the second attenuator and the first reflective mirror, then it is reflected into after the first lens reach Amici prism by the second reflecting mirror and reaches CCD, the angle of reference light and CCD plane normal direction can be adjusted by the knob on the second reflecting mirror；Object light enters the second collimator and extender device after the second half-wave plate, Amici prism is injected by the second lens, a portion object light is through Amici prism by irradiating tested sample after microcobjective, its reflected light changes direction after reaching Amici prism, normal direction along CCD plane is propagated, and interfered with reference light, interference fringe is recorded on the CCD.The configuration of the present invention is simple has good measurement accuracy.
Description
Technical field
The present invention relates to holography fields, especially pre-amplification off-axis digital holography microscopic system.
Background technique
The basic principle of digital holography is similar with previous optical holography, is also classified into record and the reproduction two of wavefront
Process.In wavefront recording process, recording beam path is consistent with optical holography, and only recording medium is replaced with electronographic device
Traditional holographic plate, and digital hologram is stored in computer.It is aobvious in optical holography during wavefront reconstruction
Shadow, fixing etc. be wet, chemical treating process and reproduces optical path then replaced the optical diffraction process completely by computer simulation.Therefore
Holographic recording, storage and the digitlization for reproducing overall process are accomplished.
Although digital holography has many advantages, such as, there is also some shortcomings, are mainly manifested in recording medium.With
Holographic plate used in traditional optical holography (resolution ratio is up to 5000 lines/mm) is compared, and is made in digital holography at present
The pel spacing of photosensitive electronographic device such as CCD is generally between 3-9um, even if newest cmos sensor,
Pel spacing also only has 1.12um, and resolution ratio is far below holographic plate.Meanwhile the photosurface size of electronographic device is also remote small
In holographic plate.Comprehensively consider space-bandwidth product, electronographic device two orders of magnitude at least poorer than holographic plate, this is just limited
The resolution ratio and object field size of digital hologram, therefore under current hardware condition, the reproduction image quality of digital hologram system
Not as good as optical holographic system.
Summary of the invention
Pre-amplification off-axis digital holography microscopic system provided by the invention, structure is simple, has good measurement accuracy.
One aspect of the present invention provides a kind of pre-amplification off-axis digital holography microscopic system, including laser, the laser
The light wave of sending reaches polarization splitting prism after the first attenuator and the first half-wave plate, and the light wave is divided into two beams, point
Not Wei reference light and object light, the reference light after the second attenuator and the first reflective mirror enter the first collimator and extender device, so
It is reflected into after the first lens reach Amici prism by the second reflecting mirror afterwards and reaches CCD, the rotation on the second reflecting mirror can be passed through
The angle of the whole reference light of button adjusting and CCD plane normal direction；The object light enters the second collimator and extender device after the second half-wave plate, leads to
It crosses the second lens and injects Amici prism, object light described in a portion is through Amici prism by irradiating detected sample after microcobjective
This, reflected light changes direction after reaching Amici prism, and the normal direction along CCD plane is propagated, and is interfered with the reference light,
Its interference fringe is recorded on the CCD, is stored in computer after acquired, quantization.
Pre-amplification off-axis digital holography microscopic system provided by the invention, the beneficial effect is that: entire measuring system tool
There is the characteristics of dynamic is good, the non-preparation of non-contact, test substance, device is simple and compact, easy to operate.
Detailed description of the invention
Fig. 1 is pre-amplification off-axis digital holography microscopic system schematic diagram of the invention；
Fig. 2 is pre-amplification partial schematic diagram.
Specific embodiment
To further illustrate that each embodiment, the present invention are provided with attached drawing.These attached drawings are that the invention discloses one of content
Point, mainly to illustrate embodiment, and the associated description of specification can be cooperated to explain the operation principles of embodiment.Cooperation ginseng
These contents are examined, those of ordinary skill in the art will be understood that other possible embodiments and advantages of the present invention.In figure
Component be not necessarily to scale, and similar component symbol is conventionally used to indicate similar component.
Now in conjunction with the drawings and specific embodiments, the present invention is further described.
Pre-amplification off-axis digital holography microscopic system provided in this embodiment, the light wave that laser 1 issues decline by first
Polarization splitting prism 4 is reached after subtracting device 2 and the first half-wave plate 3, and is divided into two beams, respectively reference light and object light, reference light
Enter the first collimator and extender device 7 after the second attenuator 5 and the first reflective mirror 6, then reflects into the by the second reflecting mirror 8
One lens 9 reach CCD11 after reaching Amici prism 10, can adjust reference light and CCD by the knob on the second reflecting mirror 8
The angle of 11 plane normal direction.Object light enters the second collimator and extender device 13 after the second half-wave plate 12, is penetrated by the second lens 14
Enter Amici prism 10, a portion object light irradiates tested sample 16 after passing through microcobjective 15 through Amici prism 10, anti-
It penetrates after light reaches Amici prism 10 and changes direction, the normal direction along 11 plane of CCD is propagated, and is interfered with reference light, is interfered
Striped is recorded on CCD11, is stored in computer after acquired, quantization.
Wherein, main devices parameter and function are as follows:
1. laser 1: using the laser of the model CL-640-050.S of Crystalaser company, wavelength is
640nm, coherence length is up to 30 meters.
2. the first attenuator 2 and the second attenuator 5: using two panels polarizer group at, by rotating one of polarizing film,
The dough softening of adjusting light consecutive variations from 0 to 1 are avoided because the light intensity for reaching CCD is too strong or excessively weak influence image quality.
3. the WPH05M-633 of the first half-wave plate 3 and the second half-wave plate 12 using Thorlabs, the use of polarization splitting prism 4
The CM1-PBS252 of Thorlabs.Wherein, it is inclined to be divided into the two mutually perpendicular lines of beam polarization state by polarization splitting prism for beam of laser
Shake light.The polarization state that Object light wave and reference light wave are adjusted by the cooperation of half-wave plate and polarization splitting prism, guarantees two-beam wave
Polarization state it is identical so that the fringe contrast of two-beam wave interference is maximum.
4. the first reflective mirror 6 and the second reflecting mirror 8: selecting newport company model is 10D20BD.1 and Thorlabs public
The reflecting mirror of model BB1-E02 is taken charge of, adaptation wave-length coverage is 488-694nm, is mainly used for changing the direction of propagation of light beam.
5. the first collimator and extender device 7 and the second collimator and extender device 13: selecting newport company model is the collimation of LC-075
Beam expanding lens, wherein incident light/emergent light diameter is up to 1.9/19mm, can expand 10 times, is mainly used for expanding two-beam wave
And collimating is directional light, guarantees to illuminate CCD completely.
6. the first lens 9 and the second lens 14: the lens for being LBF254-100 for Thorlabs company model.Wherein,
The rear focus of two lens 14 and the front focus of microcobjective are in same position, so that the light of incident object is the parallel of a branch of collimation
Light.The effect of first lens 9 is a part of microcobjective of correction and the 14 bring phase distortion of the second lens.
7. Amici prism 10: selecting Thorlabs company model is the Amici prism of CM1-BS013, and inclined-plane plates spectro-film,
Its lap plates anti-reflection film, and main function is the direction of propagation of light splitting and turnover light wave.Reference light penetrates Amici prism in the present apparatus
Reach CCD, object light is split prism turnover through the reflected light after reaching tested sample surface after Amici prism and microcobjective
Also CCD is reached, two-beam wave interferes.
8. microcobjective 15: use Mitutoyo company model for M Plan Appo SL, amplification factor be 50 × it is aobvious
Speck mirror, numerical aperture NA=0.42, focal length 200mm mainly play amplification.
9.CCD 11: the monochromatic progressive scan CCD camera that IMPERX company model is IPX-2M30-L is selected, wherein having
Effect photosensitive area is 7.46mm × 7.60mm, is exported as 10 position digital signals of 1008 (level) × 1028 (vertical), pixel ruler
Very little is 7.4 μm of 7.4 μ m, and speed is 33 frames/second.Selecting Dalsa company model is the image pick-up card of X64-CL ipro100,
Computer will be sent into after the hologram quantization of CCD acquisition.
In above-mentioned optical path, each device is fixed on two vertical plates by fixing seat.By designing various sizes of fixing seat,
Guarantee that all optical devices are coaxial.In this optical path, all optical device centre distance two vertical plates plane 46mm.To guarantee light
Two vertical plates are fixed on horizontal air floating platform by road stability.Tested sample is placed on fine motion D translation platform, Ke Yifang
Just tested sample is loaded and replaces, by the adjustable wavefront recording distance d of knob on fine motion D translation platform, or will
Selected sample is moved to viewing area.
Because joined microcobjective in object light, amplification is played to tested sample, which belongs to pre-amplification
Off-axis digital holography recording beam path, schematic diagram are as shown in Figure 2.Object AB is imaged as A' after being amplified by microcobjective at image planes
CCD plane is reached after B', propagation distance d, it should using the object of object recorded as A'B' as digital hologram system, therefore wavefront
Recording distance is distance d of the image planes to CCD plane, rather than object plane is to the distance of CCD plane.
Although specifically showing and describing the present invention in conjunction with preferred embodiment, those skilled in the art should be bright
It is white, it is not departing from the spirit and scope of the present invention defined by the appended claims, it in the form and details can be right
The present invention makes a variety of changes, and is protection scope of the present invention.
Claims (1)
1. a kind of pre-amplification off-axis digital holography microscopic system, which is characterized in that including laser, the light of the laser sending
Wave reaches polarization splitting prism after the first attenuator and the first half-wave plate, and the light wave is divided into two beams, respectively refers to
Light and object light, the reference light enter the first collimator and extender device after the second attenuator and the first reflective mirror, then by the
Two-mirror reflects into after the first lens reach Amici prism and reaches CCD, can be adjusted and be joined by the knob on the second reflecting mirror
Examine the angle of light Yu CCD plane normal direction；The object light enters the second collimator and extender device after the second half-wave plate, thoroughly by second
Mirror enters Amici prism, and object light described in a portion irradiates tested sample after passing through microcobjective through Amici prism, anti-
It penetrates after light reaches Amici prism and changes direction, the normal direction along CCD plane is propagated, and is interfered with the reference light, is interfered
Striped is recorded on the CCD, is stored in computer after acquired, quantization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710747150.2A CN109426124A (en) | 2017-08-28 | 2017-08-28 | Pre-amplification off-axis digital holography microscopic system |
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|---|---|---|---|
| CN201710747150.2A CN109426124A (en) | 2017-08-28 | 2017-08-28 | Pre-amplification off-axis digital holography microscopic system |
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| CN109426124A true CN109426124A (en) | 2019-03-05 |
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| CN201710747150.2A Pending CN109426124A (en) | 2017-08-28 | 2017-08-28 | Pre-amplification off-axis digital holography microscopic system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113960040A (en) * | 2021-10-29 | 2022-01-21 | 杭州智微信息科技有限公司 | System and method for automatically detecting and identifying plasmodium |
| CN114459342A (en) * | 2022-01-25 | 2022-05-10 | 华南师范大学 | Coaxial and off-axis digital holographic switching device based on parallel beam splitting prism |
-
2017
- 2017-08-28 CN CN201710747150.2A patent/CN109426124A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113960040A (en) * | 2021-10-29 | 2022-01-21 | 杭州智微信息科技有限公司 | System and method for automatically detecting and identifying plasmodium |
| CN114459342A (en) * | 2022-01-25 | 2022-05-10 | 华南师范大学 | Coaxial and off-axis digital holographic switching device based on parallel beam splitting prism |
| CN114459342B (en) * | 2022-01-25 | 2023-07-04 | 华南师范大学 | On-axis and off-axis digital holographic switching device based on parallel beam splitting prism |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190305 |
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| WD01 | Invention patent application deemed withdrawn after publication |