CN1094152C - Microbiological medicine containing rich selenium for health-care and therapy and its preparation - Google Patents
Microbiological medicine containing rich selenium for health-care and therapy and its preparation Download PDFInfo
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- CN1094152C CN1094152C CN941064085A CN94106408A CN1094152C CN 1094152 C CN1094152 C CN 1094152C CN 941064085 A CN941064085 A CN 941064085A CN 94106408 A CN94106408 A CN 94106408A CN 1094152 C CN1094152 C CN 1094152C
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Abstract
The present invention relates to a microbiological composition containing rich selenium for health care and therapy, which belongs to a mixture which is formed by microorganism fermentation and contains photosynthetic bacterial thallus protein, the metabolic secretions of the photosynthetic bacterial thallus, organic selenium compounds and Chinese herbal medicine yeast. The preparation method of the microbiological composition comprises the following steps: the mixture which comprises starch, inorganic selenium and Chinese herbal medicine extract is used as a culture medium, and then, the red bacterial suspended matter which is obtained by cultivating photosynthetic bacteria is further processed into various foods and medicines.
Description
The present invention relates to a kind of malignant tumour, diabetes, cardiovascular diseases, enhance immunity power, endurance, anti-chemical toxicant ability, radioprotective and chromosomal variation ability of being used for the treatment of, and the microorganism selenium preparation and the production method thereof that are used to keep healthy, be a kind of health therapy preparation that utilizes photosynthetic bacterium production to contain organoselenium and effective components of Chinese medicinal and photosynthetic bacterial thallus specifically.
The method of known synthetic organoselenium is a lot of at present, mainly divides two kinds: a kind of is biological process, as selenium yeast, selenium mushroom; Another kind is a biochemical process, and as carrageenin, thiosulfuric acid chrondroitin etc., its selenium transformation efficiency is low, and the product of conversion is single, only contains seleno-protein or k-selenocarrageenan, foretells the quinoline compounds and never appears in the newspapers and resemble seleno nucleic acid, selenium.
The inventor finds after deliberation, utilizes photosynthetic bacterium inorganic selenium can be converted into and comprises seleno-protein, k-selenocarrageenan, seleno nucleic acid, selenium fore-telling quinoline compounds.Simultaneously, photosynthetic bacterial thallus contains 18 seed amino acids, multivitamin, wherein vitamin B group B particularly
12Content be higher than yeast far away.In addition, contain ubiquinone, foretell compound and nucleic acid fragments such as quinoline compounds and carotenoid, be much better than yeast as transforming inorganic selenium.
Chinese medicine has multiple working method.As water carry, alcoholic extraction, CN93104737.4 utilizes edible fungus fermented processing herbal medicine.The inventor discovers that photosynthetic bacterium can utilize some small molecules in the herbal medicine, and the tropina of synthetic photosynthetic bacterium does not destroy efficacy component simultaneously again, and the two forms a kind of medicine abundanter more than Chinese medicinal ingredients altogether.
The objective of the invention is, a kind of method of utilizing microbial transformation inorganic selenium and processing herbal medicine is provided, the two unites two into one and just forms a kind of microorganisms producing of utilizing and be used for the treatment of malignant tumour, diabetes, cardiovascular diseases, enhance immunity power, endurance, anti-chemical toxicant ability, radioprotective and chromosomal variation ability, and the microorganism selenium preparation that is used to keep healthy.
A kind of microorganisms producing of utilizing described in the present invention is used for the treatment of malignant tumour, diabetes, cardiovascular diseases, enhance immunity power, endurance, anti-chemical toxicant ability, radioprotective and chromosomal variation ability, and the microorganism selenium preparation that is used to keep healthy.The microorganism that produces the microorganism selenium preparation is CGMCC No.0192 photosynthetic bacterium mixed culture or wherein arbitrary bacterial strain.CGMCC No.0192 photosynthetic bacterium on February 22nd, 1993 in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) preservation, wherein contain 9 strain photosynthetic bacteriums, be respectively rhodospirillum (Rhodospirillium) 1 strain, rhodopseudomonas (Rhodopseudomonas) 2 strains, spherical red bacillus (Rodobacter) 2 strains, chomophoric bacterium (Chromatium) 1 strain, chloracea (Chlorobium) 1 strain, red thiobacterium 2 strains.
The cultural method of photosynthetic bacterium mixed culture CGMCC No.0192 is: add 1gKH in the 1000ml distilled water
2PO
4, 0.5gMgCl
26H
2O, 0.1gCaCl2H
2O, 1gNH
4Cl, 3gNaHCO
3, 0.7gNa
2SO
4, 1gNaCL, 1ml do not have vitriol trace element solution and lml vitamin solution, and under conventional organic carbon source condition, regulating initial pH is 6.9, cultivates with the illumination anaerobic process, and light wave is the inclined to one side long wave of visible light, and intensity of illumination is 1000-3000Lux, culture reddens gradually.It is to survey the absorption spectrum of its culture at the 630nm place that its growth detects, and along with the growth of bacterium liquid, the absorption of its 0.D630nm increases gradually.No vitriol trace element solution is: with 1.8g FeCl
24H
2O, 25omg CoCl
26H
2O, 10mgNiCl
26H
2O, 10mg CaCl
25H
2O, 70mg MnCl
24H
2O, 100mgZnCl
2, 500mg H
3BO
3, 30mg Na
2MO
42H
2O, 10mg Na
2SeO
35H
2In the O dissolving 1000ml distilled water, the pH value of solution is adjusted to 2-3 with HCL.
Vitamin solution is to contain the 10mg vitamin H in the 100ml distilled water, 35mg niacinamide, 30mg titanium dioxide thiamines element, 20mg calcium para-aminobenzoic acid, 10mg pyridoxal hydrochloride, 10mg calcium pantothenate, 5mg vitamins B
12
Embodiment of the present invention are: the amidin of 10-30% (by weight) mixes with the bean powder of 10-30% at 1: 1; Transferring pH with 1N HCl is 2, boils 0.5-2 hour; Transfer neutral with NaOH; By the amount of every kind of herbal medicine 10 grams per liter fermented liquids take by weighing that chrysanthemum, sealwort, Wu Chia Pee, Root-bark of Chinese Wolfberry, flea are stopped, Poria cocos, water chestnut, Ootheca Mantidis, tortoiseshell, safflower, oyster, Chinese yam, wolfberry fruit, Glossy Privet Fruit, the water logging bubble 24 hours that adds 10 times of weight, poach extracting until volume-diminished to approximately till half; Mix with the starch solution after the saccharification; 10-30 ℃ anaerobic acidification 8-48 hour, make pH to 4-5, precipitate 2 hours and take out supernatant liquor; Transfer to neutrality; Add inorganic selenium, make the liquid selenium content reach 200-800ppm; Use NaHCO
3It is neutral transferring pH; At 1Kg pressure, sterilization is 30 minutes under 126 ℃ of conditions; Be cooled to room temperature, aseptic access accounts for the CGMCC No.0192 photosynthetic bacterium culturing mixt of total amount of liquid 20% or any single strain culture wherein, at 10-30 ℃, preferably 28-30 ℃, dissolved oxygen is 0.1-1ppm, 0.5ppm preferably, intensity of illumination is 1000-3000Lux, preferably cultivated 8-15 days under the condition of 2000Lux, promptly get the red malignant tumour that is used for the treatment of, diabetes, cardiovascular diseases, enhance immunity power, endurance, anti-chemical toxicant ability, radioprotective and chromosomal variation ability, and the microorganism selenium preparation that is used to keep healthy, wherein containing photosynthetic bacterial thallus quantity is 20 * 10
8-80 * 10
8Individual/ml, contain seleno-protein, k-selenocarrageenan and seleno nucleic acid, selenium fore-telling quinoline compounds four class organic selenium compounds, the organoselenium total amount is 320-724mg/l, also contains multiple efficacy components such as saponin, choline, phosphatide simultaneously.
It is the fore-telling quinoline compounds that a class contains the Se-C key that selenium among the present invention is foretold the quinoline compounds, cultured selenium is closed bacterium, under the 5000rpm condition centrifugal 20 minutes, get its throw out, be suspended from the hydrobromic acid solution of 4mol/L of 1: 5 volume, ultrasonic disruption, hydrolysis 50 hours in the environment of 50 ℃ of inflated with nitrogen again is 1: 5 tetracol phenixin extracting 24 hours again with volume ratio, takes out carbon tetrachloride layer, evaporate to dryness promptly gets selenium and foretells the quinoline compounds.
Seleno nucleic acid among the present invention is a kind of transfer RNA (tRNA) (RNA) that contains selenium, separate selenium with the method for routine and close total RNA of bacterium, separate with benzoyl Mierocrystalline cellulose (1: 1) post with the DEAE-Mierocrystalline cellulose, its elutriant is the Tris-HCL damping fluid of pH7.4, and elution speed is 1-2ml/min.
A kind of malignant tumour, diabetes, cardiovascular diseases, enhance immunity power, endurance, anti-chemical toxicant ability, radioprotective and chromosomal variation ability of being used for the treatment of in the invention, and the microorganism selenium preparation that is used to keep healthy, can reduce the damage of tetracol phenixin to liver, oxynitride and oxysulfide to lung, damage; Reduce oligoleukocythemia and chromosomal variation that the X line causes, increase the macrophage phagocytic amount of mouse, anti-small white mouse myocardial anoxia and strengthen mouse muscle power and anti-stress effect, lowering blood glucose and glycosuria, thus to protection because of chemical toxicant and radiation injury to body.All effective in cure to heart trouble, diabetes and hypoimmunity, cancer cells is also shown great restraining effect.Clinical observation through 100 people shows, its to cardiopathic efficient be 81.2%; Efficient up to 80.6% to diabetes also can make 68.8% patient obviously improve immunizing power.
Technical process of the present invention is: starch solution is transferred pH to 2 through the HCl of 1N, poach 0.5-2 hour saccharification liquid; Transfer neutral with NaOH; Herbal medicine is got extract with poach; With saccharification liquid and extract mix saccharification extracting mixed solution; At 10-30 ℃ of anaerobic acidification 8-48 hour; Transfer to neutrality, get neutral nutritive medium; Add inorganic selenium, make the liquid selenium content reach 200-800ppm, use NaHCO
3It is neutral transferring pH; Autoclaving inserts and accounts for the CGMCC No.0192 photosynthetic bacterium culture of total amount of liquid 20% or arbitrary bacterial strain wherein; At temperature 10-30 ℃, dissolved oxygen is 0.1-1ppm, intensity of illumination is to cultivate 8-15 days under the 1000-3000Lux condition, promptly get red malignant tumour, diabetes, cardiovascular diseases, enhance immunity power, endurance, anti-chemical toxicant ability, radioprotective and chromosomal variation ability and the microorganism selenium preparation that is used to keep healthy of being used for the treatment of.
Embodiment 1
Be soaked in water 8 hours with the corn that accounts for weight percent 15% and 15% soya bean, pulverizing in its liquid HCL with 1N, to transfer pH be 2, boils hydrolysis after 1 hour; Transfer to neutrality with NaOH; Chrysanthemum, sealwort, Wu Chia Pee, Root-bark of Chinese Wolfberry, flea are stopped, Poria cocos, water chestnut, Ootheca Mantidis, tortoiseshell, safflower, oyster, Chinese yam, wolfberry fruit, 14 kinds of Chinese medicines of Glossy Privet Fruit take by weighing by the amount of 10 grams per liter fermented liquids respectively, water logging with 10 times of weight was steeped 24 hours, poach extracts, till volume reduces half; Add neutral corn, soya bean hydrolyzed solution in the herb liquid this moment; 28 ℃ of acidifyings 24 hours, the pH value reduces to 4, precipitates 2 hours, takes out supernatant liquor; Transfer pH to neutral with NaOH, add the Sodium Selenite of 600ppm; Use NaHCO
3Adjust pH is neutral; 126 ℃ of sterilizations of 1Kg pressure 30 minutes; The red false unit cell (Rhodopesudomonas) among the CGMCC No.0192 that accounts for liquid gross weight 20% is inserted in the cooling back, spherical red bar (sphaeroides) culture, 28 ℃ of dissolved oxygens are 0.5ppm, intensity of illumination is to cultivate 10 days under the 2000Lux condition, promptly get red malignant tumour, diabetes, cardiovascular diseases, enhance immunity power, endurance, anti-chemical toxicant ability, radioprotective and chromosomal variation ability and the microorganism selenium preparation that is used to keep healthy of being used for the treatment of.Wherein contain organoselenium 400ppm, add medical starch, be made into capsule, every capsules 0.3g contains selenium 100 μ g.This capsule is used for patient, and every day twice, each two, one month is a course of treatment, and its 100 people's clinical effectiveness is as follows:
It is efficient that sick type example digital display is imitated enabledisable
It is efficient that sick type example digital display is imitated enabledisable
Heart trouble 32 15 11 8 81.2%
Diabetes 36 19 10 7 80.6%
Hypoimmunity 32 14 8 10 68.8%
Embodiment 2
After soybean soaking with 20%, the pulverizing, steam and boiled 5 minutes, add the terra alba that accounts for liquid weight 2%, precipitate 2 hours, get its supernatant liquor, extract herbal medicine with method identical among the embodiment 1, the soya-bean milk supernatant liquor mixes with Chinese traditional medicine water extract, under 28 ℃ of conditions, acidifying 8 hours, pH value 4.5, transfer to neutrality, add Sodium Selenite, make that selenium content reaches 800ppm in total liquid, use NaHCO
3It is neutral transferring pH; Behind the autoclaving, insert the CGMCC No.0192 photosynthetic bacterium culture account for gross weight 20%, at the 0.5ppm dissolved oxygen, the 2000Lux light intensity was cultivated 12 days for 28 ℃, promptly got to be used to the photosynthetic bacteria preparation for the treatment of and keeping healthy.
Embodiment 3
Sodium Selenite among the embodiment 2 is changed to tin anhydride, use the same method and cultivate, what obtain is photosynthetic bacteria preparation, its selenium content is 620mg/L, is processed into capsule, every 0.3 gram, contain selenium 147 μ g, its result of treatment is: heart trouble is efficient to reach 74.3%, and diabetes are efficient to be 81.3%, and improving immune efficient is 63.2%.
Claims (24)
1. the method with photosynthetic bacterium mixed culture CGMCC No.0192 processing Chinese medicine material is characterized in that: with the amidin saccharification of 10-30%; Transfer neutral; Mix with the water extract of Chinese medicine material; At 10-30 ℃ of anaerobic acidification 8-48 hour to pH4-5; Transfer neutral; Autoclaving; Access accounts for the photosynthetic bacterium mixed culture CGMCC No.0192 of fermented liquid total amount 20%; 10-30 ℃ temperature, the intensity of illumination of 1000-3000Lux, the condition bottom fermentation of 0.1-1ppm dissolved oxygen 8-15 days.
2. the product that method according to claim 1 makes.
3. the method with the synthetic organoselenium of photosynthetic bacterium mixed culture CGMCC No.0192 is characterized in that: with the amidin saccharification of 10-30%; Transfer neutral; At 10-30 ℃ of anaerobic acidification 8-48 hour to pH4-5; Transfer neutral; Add the 200-800ppm inorganic selenium; Transfer neutral; Autoclaving; Access accounts for the photosynthetic bacterium mixed culture CGMCC No.0192 of fermented liquid total amount 20%; 10-30 ℃ temperature, the intensity of illumination of 1000-3000Lux, the dissolved oxygen condition bottom fermentation of 0.1-1ppm 8-15 days.
4. the product that method according to claim 3 makes.
5. a method for preparing the microorganism selenium preparation with photosynthetic bacterium mixed culture CGMCC No.0192 is characterized in that: with the amidin saccharification of 10-30%; Transfer neutral; Amount at 10 grams per liter fermented liquids takes by weighing the Chinese medicine material chrysanthemum, sealwort, and Wu Chia Pee, Root-bark of Chinese Wolfberry, flea stops, Poria cocos, the ground water chestnut, Ootheca Mantidis, tortoise version, safflower, oyster, Chinese yam, wolfberry fruit, Glossy Privet Fruit adds the water logging bubble 24 hours of 10 times of weight, boil to volume-diminished half, the water extract of Chinese medicine material; Amidin after the saccharification is mixed with the water extract of Chinese medicine material; 10-30 ℃ anaerobic acidification 8-48 hour to pH4-5; Transfer neutral; Add the 200-800ppm inorganic selenium; Transfer neutral; Autoclaving; Access accounts for the photosynthetic bacterium mixed culture CGMCC No.0192 of fermented liquid total amount 20%; 10-30 ℃ temperature, the intensity of illumination of 1000-3000Lux, the dissolved oxygen condition bottom fermentation of 0.1-1ppm 8-15 days.
6. according to claim 1 or 3 or 5 described methods, amidin wherein is that W-Gum and bean powder mix at 1: 1.
7. according to claim 1 or 3 or 5 described methods, saccharification wherein is to transfer pH to 2, poach 0.5-2 hour with the hydrochloric acid of 1N.
8. according to claim 1 or 3 or 5 described methods, wherein autoclaved temperature is 1 26 ℃, and pressure is 1 kilogram.
9. according to claim 1 or 3 or 5 described methods, photosynthetic bacterium mixed culture CGMCC No.0192 wherein can use wherein arbitrary bacterial strain to replace.
10. according to claim 3 or 5 described methods, inorganic selenium wherein is tin anhydride or Sodium Selenite.
11. the microorganism selenium preparation that makes according to any one described method among the claim 5-10.
12. microorganism selenium preparation according to claim 11 wherein contains photosynthetic bacterial thallus albumen, organic selenium compounds and herbal medicine efficacy composition.
13. microorganism selenium preparation according to claim 11 wherein contains 20 * 10
8-80 * 10
8Individual/ml photosynthetic bacterium and meta-bolites seleno-protein thereof, k-selenocarrageenan, seleno nucleic acid, selenium are foretold the quinoline compounds.
14. according to claim 2 or 4 or 11 described products, photosynthetic bacterium mixed culture CGMCC No.0192 wherein can use wherein arbitrary bacterial strain to replace.
15. microorganism selenium preparation according to claim 11 is in the medicine of preparation treatment malignant tumour and the purposes in the healthcare products.
16. microorganism selenium preparation according to claim 11 is in the medicine of preparation treatment diabetes and the purposes in the healthcare products.
17. microorganism selenium preparation according to claim 11 is in the medicine of preparation treatment cardiovascular diseases and the purposes in the healthcare products.
18. microorganism selenium preparation according to claim 11 is in the medicine of preparation enhance immunity power and the purposes in the healthcare products.
19. microorganism selenium preparation according to claim 11 improves the medicine of body endurance and the purposes in the healthcare products in preparation.
20. microorganism selenium preparation according to claim 11 improves the medicine of the anti-chemical toxicant ability of body and the purposes in the healthcare products in preparation.
21. the purposes of microorganism selenium preparation according to claim 11 in radiation-resistant medicine of preparation and healthcare products.
22. microorganism selenium preparation according to claim 11 is in the medicine of the anti-chromosomal variation ability of preparation and the purposes in the healthcare products.
23. foretell the quinoline compounds according to the selenium that any one described method among claim 3 or the 5-10 obtains.
24. the seleno nucleic acid that obtains according to any one described method among claim 3 or the 5-10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN941064085A CN1094152C (en) | 1994-06-21 | 1994-06-21 | Microbiological medicine containing rich selenium for health-care and therapy and its preparation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN941064085A CN1094152C (en) | 1994-06-21 | 1994-06-21 | Microbiological medicine containing rich selenium for health-care and therapy and its preparation |
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| Publication Number | Publication Date |
|---|---|
| CN1108944A CN1108944A (en) | 1995-09-27 |
| CN1094152C true CN1094152C (en) | 2002-11-13 |
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| CN941064085A Expired - Fee Related CN1094152C (en) | 1994-06-21 | 1994-06-21 | Microbiological medicine containing rich selenium for health-care and therapy and its preparation |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1087781C (en) * | 1998-05-28 | 2002-07-17 | 济南三株药业有限公司 | Bioconversion method of selenium and zinc by utilizing beneficial bacterium |
| CN101139554B (en) * | 2007-09-14 | 2011-05-18 | 梁林 | Solid-state fermentation preparing method for Chinese herbal medicine yeast selenium |
| CN102917715B (en) | 2010-02-16 | 2015-07-01 | Tfk株式会社 | Prophylactic/ameliorating agent for diseases, endurance improving agent and anti-fatigue agent, and pharmaceutical agent, food and beverage each comprising any one of those agents |
| CN101990989A (en) * | 2010-12-03 | 2011-03-30 | 井良才 | Selenium-rich eggs |
| CN108721337B (en) * | 2017-04-17 | 2022-05-10 | 江苏德禧生物科技有限公司 | Microbial agent for preventing tumor chemotherapy intestinal toxicity |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1064888A (en) * | 1992-04-27 | 1992-09-30 | 萧河龙 | Edible mushrooms and herbal medicine mixed fermentation brewing technology |
| CN1085432A (en) * | 1993-05-03 | 1994-04-20 | 王强华 | A kind of manufacture method of rich selenium powder |
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1994
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1064888A (en) * | 1992-04-27 | 1992-09-30 | 萧河龙 | Edible mushrooms and herbal medicine mixed fermentation brewing technology |
| CN1085432A (en) * | 1993-05-03 | 1994-04-20 | 王强华 | A kind of manufacture method of rich selenium powder |
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| CN1108944A (en) | 1995-09-27 |
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