CN109414008A - 防止重结晶的冷冻保存培养基和方法 - Google Patents

防止重结晶的冷冻保存培养基和方法 Download PDF

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Publication number
CN109414008A
CN109414008A CN201780028522.4A CN201780028522A CN109414008A CN 109414008 A CN109414008 A CN 109414008A CN 201780028522 A CN201780028522 A CN 201780028522A CN 109414008 A CN109414008 A CN 109414008A
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CN
China
Prior art keywords
culture medium
cell
liquid
cryogenic
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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CN201780028522.4A
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English (en)
Chinese (zh)
Inventor
韩旭
袁野
R.M.罗伯茨
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University of Missouri St Louis
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University of Missouri St Louis
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Publication of CN109414008A publication Critical patent/CN109414008A/zh
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/128—Chemically defined matrices for immobilising, holding or storing living parts, e.g. alginate gels; Chemically altering living parts, e.g. by cross-linking
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L71/00—Compositions of polyethers obtained by reactions forming an ether link in the main chain; Compositions of derivatives of such polymers
    • C08L71/02—Polyalkylene oxides
    • C08L71/03—Polyepihalohydrins

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dispersion Chemistry (AREA)
CN201780028522.4A 2016-05-13 2017-05-15 防止重结晶的冷冻保存培养基和方法 Pending CN109414008A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662336142P 2016-05-13 2016-05-13
US62/336,142 2016-05-13
PCT/US2017/032606 WO2017197379A1 (en) 2016-05-13 2017-05-15 Cryopreservation medium and method to prevent recrystallization

Publications (1)

Publication Number Publication Date
CN109414008A true CN109414008A (zh) 2019-03-01

Family

ID=60267661

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780028522.4A Pending CN109414008A (zh) 2016-05-13 2017-05-15 防止重结晶的冷冻保存培养基和方法

Country Status (5)

Country Link
US (1) US20190313632A1 (https=)
EP (1) EP3454653A4 (https=)
JP (1) JP2019514442A (https=)
CN (1) CN109414008A (https=)
WO (1) WO2017197379A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117177663A (zh) * 2021-04-05 2023-12-05 库瑞欧库瑞特有限公司 消除了对细胞渗透性防冻剂的需要的高效生物相容性冷冻保存培养基

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201716729D0 (en) * 2017-10-12 2017-11-29 Asymptote Ltd Cryopreservation method and apparatus
JP7391528B2 (ja) * 2019-02-15 2023-12-05 イビデン株式会社 凍結保存用高分子水溶液
WO2020198693A1 (en) * 2019-03-27 2020-10-01 EMULATE, Inc. Microfluidic devices for tattoo pigment safety
WO2021159073A1 (en) 2020-02-06 2021-08-12 The George Washington University Methods and compositions for cryopreservation of cell therapies
US12426594B2 (en) 2020-09-24 2025-09-30 Everest Medical Innovation GmbH Cryoprotective compositions and methods for protection of a surgical site during cryosurgery
US12453805B2 (en) 2020-09-24 2025-10-28 Everest Medical Innovation GmbH Cryoprotective compositions, surgical kits, and methods for protection of a surgical site during cryosurgery
CN112293411B (zh) * 2020-11-27 2021-09-28 大连海洋大学 中间球海胆精子的低温保存液及保存方法
CN113491267A (zh) * 2021-07-22 2021-10-12 南昌市生殖医院 一种微量精子冷冻保存载体及相应的冷冻和解冻方法
WO2025019400A1 (en) * 2023-07-14 2025-01-23 Cryocrate Llc Efficient cryopreservation medium that preserves tissue ultra-structures and eliminates tissue cells

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5171661A (en) * 1988-05-18 1992-12-15 Cryopharm Corporation Medium for lyophilization of erythrocytes
US5629145A (en) * 1995-03-24 1997-05-13 Organ, Inc. Cryopreservation of cell suspensions
US20020051963A1 (en) * 1999-03-05 2002-05-02 Victor Bronshtein Shelf preservation of cells, tissues, organs and organisms by vitrification
WO2013096659A1 (en) * 2011-12-20 2013-06-27 Cook General Biotechnology Llc Methods and compositions for storage of animal cells
CN103184189A (zh) * 2011-12-29 2013-07-03 北京富通华生物科技有限公司 一种杂交和牛的培育方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4362756A (en) * 1981-12-28 1982-12-07 Borden, Inc. Brown sugar sweetened condensed milk and process for preparation thereof
US20060051731A1 (en) * 2004-08-12 2006-03-09 David Ho Processes for preparing lyophilized platelets
JP5553332B2 (ja) * 2009-08-12 2014-07-16 独立行政法人理化学研究所 生物材料用ガラス化液、ガラス化キット、及びその利用
CN102657152B (zh) * 2012-05-04 2014-06-25 南京林业大学 一种胚性材料的超低温冷冻保存方法
CN104988115A (zh) * 2015-07-14 2015-10-21 中国科学院过程工程研究所 喹啉或异喹啉类小分子化合物在人多能干细胞培养及冷冻保存中的应用

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5171661A (en) * 1988-05-18 1992-12-15 Cryopharm Corporation Medium for lyophilization of erythrocytes
US5629145A (en) * 1995-03-24 1997-05-13 Organ, Inc. Cryopreservation of cell suspensions
US20020051963A1 (en) * 1999-03-05 2002-05-02 Victor Bronshtein Shelf preservation of cells, tissues, organs and organisms by vitrification
WO2013096659A1 (en) * 2011-12-20 2013-06-27 Cook General Biotechnology Llc Methods and compositions for storage of animal cells
CN103184189A (zh) * 2011-12-29 2013-07-03 北京富通华生物科技有限公司 一种杂交和牛的培育方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117177663A (zh) * 2021-04-05 2023-12-05 库瑞欧库瑞特有限公司 消除了对细胞渗透性防冻剂的需要的高效生物相容性冷冻保存培养基

Also Published As

Publication number Publication date
EP3454653A4 (en) 2020-01-22
US20190313632A1 (en) 2019-10-17
EP3454653A1 (en) 2019-03-20
WO2017197379A1 (en) 2017-11-16
JP2019514442A (ja) 2019-06-06

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Application publication date: 20190301