CN109381703A - Calcium alginate nanofiber and preparation method and application carry medicine calcium alginate nanofiber and preparation method - Google Patents
Calcium alginate nanofiber and preparation method and application carry medicine calcium alginate nanofiber and preparation method Download PDFInfo
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- CN109381703A CN109381703A CN201811426101.XA CN201811426101A CN109381703A CN 109381703 A CN109381703 A CN 109381703A CN 201811426101 A CN201811426101 A CN 201811426101A CN 109381703 A CN109381703 A CN 109381703A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/02—Inorganic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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Abstract
The present invention provides a kind of calcium alginate nanofiber and preparation method and application, carry medicine calcium alginate nanofiber and preparation method, it is related to nanofiber technology field, the calcium alginate nanofiber is prepared using micro-fluidic spinning, existing calcium alginate nanofiber is alleviated to be made by wet spinning, there are grooves on surface, intensity is caused to decline, the technical issues of limiting its scope of application, calcium alginate nanofiber provided by the invention is prepared by micro-fluidic spinning, simple process, it is easy to operate, a large amount of man power and material can be saved, and the calcium alginate nanofiber surface being prepared is smooth, without groove, it ensure that the intensity of nanofiber, expand the application range of calcium alginate nanofiber.
Description
Technical field
The present invention relates to nanofiber technology field, more particularly, to a kind of calcium alginate nanofiber and preparation method and
Using, carry medicine calcium alginate nanofiber and preparation method.
Background technique
It improves with the development of science and technology, has the progress and breakthrough of huge leap forward in terms of the biology of medicine, all over the world
Scientist all fall over each other research and develop biological species medical material, it is seen that bio-medical material has been a big research hotspot.Wherein, high
Molecular biosciences medical material has obtained good application in biomedical field, is one of application material the most universal.
In recent years, natural polymer bio-medical material was even more to become biological medical polymer material research and development
Emphasis, be especially to represent marine organisms medical material since its is resourceful with alginate, and biological safety is good,
By living nature extensive concern, especially there is good gel blockage and hemostasis using the medical dressing that calcium alginate is prepared as raw material
Effect facilitates wound hemostasis and promotes hemostatic factor growth.But traditional calcium alginate fibre is made using wet spinning,
Leading to fiber surface, there are some grooves, reduce the intensity of fiber, limit its scope of application.
In view of this, the present invention is specifically proposed.
Summary of the invention
One of the objects of the present invention is to provide a kind of preparation methods of calcium alginate nanofiber, to alleviate existing sea
Calcium alginate nanofiber is made by wet spinning, and there are grooves on surface, cause intensity to decline, the skill limited its scope of application
Art problem.
The preparation method of calcium alginate nanofiber provided by the invention, is prepared using micro-fluidic spinning.
Further, include the following steps:
(a) sodium alginate soln is delivered to wire vent channel by first passage；
(b) calcium chloride solution is delivered to wire vent channel from second channel, so that calcium alginate solution and calcium chloride solution
In the raw calcium ion-exchanged reaction of wire vent channel hybrid concurrency, calcium alginate solution is generated；
(c) calcium alginate solution is squeezed out from slinning cabinet, obtains calcium alginate nanofiber.
Further, in step (a), the flow velocity of sodium alginate soln is 0.5-1.5mL/h, preferably 1mL/h；
And/or in step (b), the flow velocity of calcium chloride solution is 40-120mL/h, preferably 50mL/h.
Further, the mass concentration of sodium alginate soln be 2.5-6%, preferably 4%；
And/or the mass concentration of calcium chloride solution be 1-3%, preferably 2%.
Further, the sodium alginate soln and/or the calcium chloride solution are pushed using Pneumatic pressure power.
The second object of the present invention is to provide a kind of calcium alginate nanofiber, uses alginic acid provided by the invention
The preparation method of calcium nanofiber is prepared.
The third object of the present invention is to provide calcium alginate nanofiber provided by the invention in pharmaceutical carrier or medical
Application in dressing.
The fourth object of the present invention is to provide a kind of load medicine calcium alginate nanofiber, including calcium alginate nanofiber
And drug, drug loading is on calcium alginate nanofiber.
The fifth object of the present invention is to provide the preparation method of above-mentioned load medicine calcium alginate nanofiber, and use is micro-fluidic
Spinning is prepared；
Preferably, include the following steps:
(A) drug is dissolved in seaweed acid solution, obtains the mixed solution of drug and sodium alginate；
(B) mixed solution of drug and sodium alginate is delivered to wire vent channel by first passage；
(C) calcium chloride solution is delivered to wire vent channel by second channel；
(D) mixed solution and calcium chloride solution of drug and sodium alginate mix in wire vent channel, and calcium ion-exchanged occurs,
Obtain the calcium alginate solution that load has drug；
(E) load has the calcium alginate solution of drug to squeeze out from slinning cabinet, obtains carrying medicine calcium alginate nanofiber.
Further, in step (B), the flow velocity of the mixed solution of drug and sodium alginate is 0.5-1.5mL/h, preferably
For 1mL/h；
And/or in step (C), the flow velocity of calcium chloride solution is 40-120mL/h, preferably 50mL/h；
And/or in the mixed solution of drug and sodium alginate, the mass concentration of calcium alginate is 2.5-6%, preferably
4%；
And/or the mass concentration of calcium chloride solution be 1-3%, preferably 2%.
Calcium alginate nanofiber provided by the invention is prepared by micro-fluidic spinning, and simple process is easy to operate,
A large amount of man power and material can be saved, and the calcium alginate nanofiber surface being prepared is smooth, no groove ensure that and receive
The intensity of rice fiber, expands the application range of calcium alginate nanofiber.
Calcium alginate nanofiber surface provided by the invention is smooth, and no groove, intensity is effectively ensured, and expands and answers
Use range.
Load medicine calcium alginate nanofiber provided by the invention by by drug loading on calcium alginate nanofiber, energy
The rate of release of drug is enough effectively reduced, extends the release time of drug, improves the curative effect of drug.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, it is described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 is the SEM figure for the calcium alginate nanofiber that the embodiment of the present invention 1 provides；
Fig. 2 is the SEM figure for the calcium alginate nanofiber that the embodiment of the present invention 2 provides；
Fig. 3 is the SEM figure for the calcium alginate nanofiber that the embodiment of the present invention 3 provides；
Fig. 4 is the SEM figure for the calcium alginate nanofiber that the embodiment of the present invention 4 provides；
Fig. 5 is the SEM figure for the calcium alginate nanofiber that the embodiment of the present invention 5 provides；
Fig. 6 is the SEM figure for the calcium alginate nanofiber that the embodiment of the present invention 6 provides；
Fig. 7 is the SEM figure for the calcium alginate nanofiber that the embodiment of the present invention 7 provides；
Fig. 8 is the SEM figure for the calcium alginate nanofiber that the embodiment of the present invention 8 provides；
Fig. 9 is the SEM figure for the calcium alginate nanofiber that the embodiment of the present invention 9 provides.
Specific embodiment
Technical solution of the present invention will be clearly and completely described below, it is clear that described embodiment is this hair
Bright a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not having
Every other embodiment obtained under the premise of creative work is made, shall fall within the protection scope of the present invention.
According to an aspect of the present invention, the present invention provides a kind of preparation methods of calcium alginate nanofiber, use
Micro-fluidic spinning is prepared.
Calcium alginate nanofiber provided by the invention is prepared by micro-fluidic spinning, and simple process is easy to operate,
A large amount of man power and material can be saved, and the calcium alginate nanofiber surface being prepared is smooth, no groove ensure that and receive
The intensity of rice fiber, expands the application range of calcium alginate nanofiber.
In the preferred embodiment of the present invention, calcium alginate Nanowire is prepared using binary channels micro-fluidic chip
Dimension.
Binary channels micro-fluidic chip includes first passage, second channel and wire vent channel, wherein first passage and second leads to
Road is connected with wire vent channel respectively.
In the preferred embodiment of the present invention, the preparation method of calcium alginate nanofiber includes the following steps:
(a) sodium alginate soln is delivered to wire vent channel by first passage；
(b) calcium chloride solution is delivered to wire vent channel from second channel, so that calcium alginate solution and calcium chloride solution
In the raw calcium ion-exchanged reaction of wire vent channel hybrid concurrency, calcium alginate solution is generated；
(c) calcium alginate solution is squeezed out from slinning cabinet, obtains calcium alginate nanofiber.
In the preferred embodiment of the present invention, in step (a), the flow velocity of sodium alginate soln is 0.5-
1.5mL/h。
In a preferred embodiment of the invention, the typical but non-limiting flow velocity for example 0.5 of sodium alginate soln,
0.6,0.7,0.8,0.9,1,1.1,1.2,1.3,1.4 or 1.5mL/h.
By controlling the flow velocity of sodium alginate soln so that the calcium alginate nanofiber surface being prepared is smooth, nothing
Groove.
In the preferred embodiment of the present invention, in step (b), the flow velocity of calcium chloride solution is 10-30mL/h.
In a preferred embodiment of the invention, the typical but non-limiting flow velocity for example 10 of calcium chloride solution, 12,
15,18,20,22,25,28 or 30mL/h.
By controlling the flow velocity of calcium chloride solution, can be handed over completely by calcium ion with the sodium ion guaranteed in sodium alginate
It changes, to obtain calcium alginate nanofiber.
In the preferred embodiment of the present invention, the mass concentration of sodium alginate soln is 2.5-6%.
In a preferred embodiment of the invention, the typical but non-limiting mass concentration of sodium alginate soln is for example
2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5% or 6%.
By controlling the concentration of sodium alginate soln, to guarantee that calcium alginate nanofiber surface obtained is smooth, no ditch
Slot, to guarantee the intensity of calcium alginate nanofiber.
In the preferred embodiment of the present invention, the mass concentration of calcium chloride solution is 1-3%.
In a preferred embodiment of the invention, the typical but non-limiting mass concentration for example 1% of calcium chloride solution,
1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 2.8% or 3%.
By controlling the concentration of calcium chloride solution so that the sodium ion in sodium alginate soln is all carried out with calcium ion
Ion exchange obtains calcium alginate nanofiber.
In the preferred embodiment of the present invention, sodium alginate soln and calcium chloride solution are independent using gas
Pressure power pushes, and the flow velocity to guarantee sodium alginate soln and calcium chloride solution is more stable.
According to the second aspect of the invention, it the present invention provides a kind of calcium alginate nanofiber, is mentioned using the present invention
The preparation method of the calcium alginate nanofiber of confession is prepared.
Calcium alginate nanofiber surface provided by the invention is smooth, and no groove, intensity is effectively ensured, and expands and answers
Curative effect is improved especially as pharmaceutical carrier in use, can effectively slow down the rate of release of drug with range.
According to the third aspect of the present invention, the present invention provides calcium alginate nanofibers in pharmaceutical carrier or Medical coating
Application in material.
According to the fourth aspect of the present invention, the present invention provides a kind of load medicine calcium alginate nanofibers, including seaweed
Sour nanofiber and drug, drug loading is on calcium alginate nanofiber.
Load medicine calcium alginate nanofiber provided by the invention by by drug loading on calcium alginate nanofiber, energy
The rate of release of drug is enough effectively reduced, extends the release time of drug, improves the curative effect of drug.
According to the fifth aspect of the present invention, the present invention provides a kind of preparation sides for carrying medicine calcium alginate nanofiber
Method is prepared using micro-fluidic spinning.
It is prepared using micro-fluidic spinning and carries medicine calcium alginate nanofiber simple process, it is easy to operate, it can save a large amount of
Man power and material.
In the preferred embodiment of the present invention, the preparation method for carrying medicine calcium alginate nanofiber includes following step
It is rapid:
(A) drug is dissolved in seaweed acid solution, obtains the mixed solution of drug and sodium alginate；
(B) mixed solution of drug and sodium alginate is delivered to wire vent channel by first passage；
(C) calcium chloride solution is delivered to wire vent channel by second channel；
(D) mixed solution and calcium chloride solution of drug and sodium alginate mix in wire vent channel, and calcium ion-exchanged occurs
Reaction obtains the calcium alginate solution that load has drug；
(E) load has the calcium alginate solution of drug to squeeze out from slinning cabinet, obtains carrying medicine calcium alginate nanofiber.
Calcium ion-exchanged is carried out by the way that drug to be dissolved in sodium alginate soln, then by sodium alginate, obtains carrying medicine
Calcium alginate nanofiber.
In the preferred embodiment of the present invention, in step (B), the stream of the mixed solution of drug and sodium alginate
Speed is 0.5-1.5mL/h.
In a preferred embodiment of the invention, the typical but non-limiting stream of the mixed solution of drug and sodium alginate
For example 0.5,0.6,0.7,0.8,0.9,1,1.1,1.2,1.3,1.4 or 1.5mL/h of speed.
By the flow velocity of control drug and the mixed solution of sodium alginate so that the calcium alginate Nanowire being prepared
Dimension table face is smooth, no groove, to reduce the rate of release of drug, improves the therapeutic effect of drug.
In the preferred embodiment of the present invention, in step (C), the flow velocity of calcium chloride solution is 40-120mL/
h。
In a preferred embodiment of the invention, the typical but non-limiting flow velocity for example 40 of calcium chloride solution, 45,
50,55,60,65,70,80,90,100,110 or 120mL/h.
By controlling the flow velocity of calcium chloride solution, can be handed over completely by calcium ion with the sodium ion guaranteed in sodium alginate
It changes, to obtain calcium alginate nanofiber.
In the preferred embodiment of the present invention, in the mixed solution of drug and sodium alginate, the matter of sodium alginate
Amount concentration is 2.5-6%.
In a preferred embodiment of the invention, in mixed solution, the typical but non-limiting quality of sodium alginate is dense
Degree for example 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5% or 6%.
By the concentration of sodium alginate soln in control mixed solution, to guarantee load medicine calcium alginate nanofiber obtained
Smooth surface, no groove improve the slow controlled-release effect of drug to guarantee the intensity of load medicine calcium alginate nanofiber.
In the preferred embodiment of the present invention, the mass concentration of calcium chloride solution is 1-3%.
In a preferred embodiment of the invention, the typical but non-limiting mass concentration for example 1% of calcium chloride solution,
1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 2.8% or 3%.
Technical solution provided by the invention is further described below with reference to embodiment and comparative example.
Embodiment 1
A kind of calcium alginate nanofiber is present embodiments provided, uses binary channels micro-fluidic chip in accordance with the following steps
It is prepared:
(1) sodium alginate soln that mass concentration is 4% is delivered to wire vent by first passage with the flow velocity of 0.5mL/h
Channel；
(2) calcium chloride solution that mass concentration is 2% wire vent is delivered to by second channel with the flow velocity of 10mL/h to lead to
Road；
(3) sodium alginate soln and calcium chloride solution mix in wire vent channel occurs calcium ion-exchanged, and logical by wire vent
Road squeezes out, and freeze-drying obtains calcium alginate nanofiber.
Embodiment 2
Present embodiments provide a kind of calcium alginate nanofiber, preparation method difference from example 1 is that,
In step (1), the flow velocity of sodium alginate soln is 1.5mL/h, and in step (2), the flow velocity of calcium chloride solution is 30mL/h.
Embodiment 3
Present embodiments provide a kind of calcium alginate nanofiber, preparation method difference from example 1 is that,
In step (1), the flow velocity of sodium alginate soln is 1mL/h, and in step (2), the flow velocity of calcium chloride solution is 20mL/h.
Embodiment 4
Present embodiments provide a kind of calcium alginate nanofiber, preparation method and embodiment 3 the difference is that,
In step (1), the flow velocity of sodium alginate soln is 10mL/h.
Embodiment 5
Present embodiments provide a kind of calcium alginate nanofiber, preparation method and embodiment 3 the difference is that,
In step (2), the flow velocity of calcium chloride solution is 50mL/h.
Embodiment 6
Present embodiments provide a kind of calcium alginate nanofiber, preparation method and embodiment 3 the difference is that,
The mass concentration of sodium alginate soln is 2.5%, and the mass concentration of calcium chloride solution is 1%.
Embodiment 7
Present embodiments provide a kind of calcium alginate nanofiber, preparation method and embodiment 3 the difference is that,
The mass concentration of sodium alginate soln is 6%, and the mass concentration of calcium chloride solution is 3%.
Embodiment 8
Present embodiments provide a kind of calcium alginate nanofiber, preparation method and embodiment 3 the difference is that,
The mass concentration of sodium alginate soln is 10%, and the mass concentration of calcium chloride solution is 6%.
Embodiment 9
Present embodiments provide a kind of calcium alginate nanofiber, preparation method and embodiment 3 the difference is that,
In step (1), the mass concentration of sodium alginate soln is 1%, and the mass concentration of calcium chloride solution is 0.5%.
Embodiment 10
Embodiment 10 provides a kind of load medicine calcium alginate nanofiber, is prepared in accordance with the following steps:
(1) rhodamine B and mixed solution of sodium alginate wire vent is delivered to by first passage with the flow velocity of 1mL/h to lead to
Road；Wherein in mixed solution, the mass concentration of sodium alginate is 4%, and the mass concentration of rhodamine is 1%；
(2) calcium chloride solution that mass concentration is 2% wire vent is delivered to by second channel with the flow velocity of 10mL/h to lead to
Road；
(3) drug and mixed solution of sodium alginate and calcium chloride solution mix in wire vent channel so that sodium alginate and chlorination
Calcium ion-exchanged occurs for calcium, and is squeezed out by wire vent channel, obtains calcium alginate nanofiber.
Embodiment 11
Present embodiments provide a kind of load medicine calcium alginate nanofiber, with implement 10 the difference is that, in step
Suddenly in (1), the mass concentration of sodium alginate is 3%.
Embodiment 12
Present embodiments provide a kind of load medicine calcium alginate nanofiber, with implement 10 the difference is that, in step
Suddenly in (1), the mass concentration of sodium alginate is 5%.
Embodiment 13
Present embodiments provide a kind of load medicine calcium alginate nanofiber, with implement 10 the difference is that, in step
Suddenly in (1), the mass concentration of sodium alginate is 2.5%.
Embodiment 14
Present embodiments provide a kind of load medicine calcium alginate nanofiber, with implement 10 the difference is that, in step
Suddenly in (1), the mass concentration of sodium alginate is 6%.
Test example 1
The calcium alginate nanofiber that embodiment 1-9 is provided is scanned Electronic Speculum detection, as shown in figs 1-9.
From Fig. 1-3 as can be seen that embodiment 1-3 provide calcium alginate nanofiber good moldability, uniform diameter, due to
Dehydration is lyophilized, there is certain buckling phenomena on surface, but surface is without groove.
It can be seen that from Fig. 4-5 when the flow velocity of the flow velocity of sodium alginate soln or calcium chloride solution is too fast, obtained sea
Mosanom fiberizing is poor, membranaceous in fiber, this illustrates that the flow velocity for controlling sodium alginate soln is 0.5-1.5mL/h, chlorination
The flow velocity of calcium solution is 10-30mL/h, and the mouldability of obtained calcium alginate nanofiber is best, and comprehensive performance is the most excellent.
From the comparison of Fig. 3, Fig. 6 and Fig. 7 and Fig. 8-9 as can be seen that when the mass concentration of sodium alginate soln is 2.5-6%
When, when the mass concentration of calcium chloride solution is 1-3%, calcium alginate nanofiber mouldability, the performance of nanofiber is more excellent
It is different.
Test example 2
The medicament-carrying nano-fiber for taking 100mg embodiment 10-14 to provide respectively, measures its drugloading rate and different time points respectively
Drug release rate, the results are shown in Table 1.
1 medicament-carrying nano-fiber performance data table of table
As it can be seen from table 1 the medicament-carrying nano-fiber that embodiment 10-14 is provided can effectively extend the release speed of drug
Rate, this illustrates that medicament-carrying nano-fiber provided by the invention has the function of medicine sustained and controlled release, can effectively improve the treatment effect of drug
Fruit.
As can also be seen from Table 1, the drug-eluting total amount that embodiment 10-12 provides medicament-carrying nano-fiber is significantly higher than implementation
Example 13-14, drug release rate is substantially less than embodiment 13-14, when this illustrates to prepare medicament-carrying nano-fiber, when sodium alginate is molten
When the mass concentration of liquid is 2.5-6%, drugloading rate and the drug controlled-releasing function for carrying medicine calcium alginate nanofiber are more excellent.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (10)
1. a kind of preparation method of calcium alginate nanofiber, which is characterized in that be prepared using micro-fluidic spinning.
2. preparation method according to claim 1, which is characterized in that prepare calcium alginate using binary channels micro-fluidic chip
Nanofiber includes the following steps:
(a) sodium alginate soln is delivered to wire vent channel by first passage；
(b) calcium chloride solution is delivered to wire vent channel from second channel, so that calcium alginate solution and calcium chloride solution are going out
The raw calcium ion-exchanged of silk channel hybrid concurrency, generates calcium alginate solution；
(c) calcium alginate solution is squeezed out from slinning cabinet, obtains calcium alginate nanofiber.
3. preparation method according to claim 2, which is characterized in that in step (a), the flow velocity of sodium alginate soln is
0.5-1.5mL/h, preferably 1mL/h；
And/or in step (b), the flow velocity of calcium chloride solution is 40-120mL/h, preferably 50mL/h.
4. preparation method according to claim 2, which is characterized in that the mass concentration of sodium alginate soln is 2.5-6%,
Preferably 4%；
And/or the mass concentration of calcium chloride solution be 1-3%, preferably 2%.
5. according to the described in any item preparation methods of claim 2-4, which is characterized in that the sodium alginate soln and/or institute
Calcium chloride solution is stated to push using Pneumatic pressure power.
6. a kind of calcium alginate nanofiber, which is characterized in that use the described in any item calcium alginate nanometers of claim 1-5
The preparation method of fiber is prepared.
7. calcium alginate nanofiber that preparation method according to claim 1-5 obtains or according to claim
Application of the calcium alginate nanofiber in pharmaceutical carrier or medical dressing described in 6.
8. a kind of load medicine calcium alginate nanofiber, which is characterized in that including calcium alginate nanofiber as claimed in claim 6
And drug, the drug loading is on the calcium alginate nanofiber.
9. the preparation method according to claim 8 for carrying medicine calcium alginate nanofiber, which is characterized in that using micro-fluidic
Spinning is prepared；
Preferably, include the following steps:
(A) drug is dissolved in seaweed acid solution, obtains the mixed solution of drug and sodium alginate；
(B) mixed solution of drug and sodium alginate is delivered to wire vent channel by first passage；
(C) calcium chloride solution is delivered to wire vent channel by second channel；
(D) mixed solution and calcium chloride solution of drug and sodium alginate mix in wire vent channel, and calcium ion-exchanged reaction occurs,
Obtain the calcium alginate solution that load has drug；
(E) load has the calcium alginate solution of drug to squeeze out from slinning cabinet, obtains carrying medicine calcium alginate nanofiber.
10. preparation method according to claim 9, which is characterized in that in step (B), drug and sodium alginate it is mixed
The flow velocity for closing solution is 0.5-1.5mL/h, preferably 1mL/h；
And/or in step (C), the flow velocity of calcium chloride solution is 40-120mL/h, preferably 50mL/h；
And/or in the mixed solution of drug and sodium alginate, the mass concentration of calcium alginate is 2.5-6%, preferably 4%；
And/or the mass concentration of calcium chloride solution be 1-3%, preferably 2%.
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| CN111139551A (en) * | 2019-12-31 | 2020-05-12 | 青岛大学 | Preparation method of skin-core structure intelligent color-changing calcium alginate fiber |
| CN113244438A (en) * | 2021-04-29 | 2021-08-13 | 五邑大学 | Preparation method of three-dimensional functional medical dressing for diabetic foot ulcer |
| CN113350562A (en) * | 2021-06-04 | 2021-09-07 | 南京鼓楼医院 | Preparation method and application of gellan gum bionic microfiber doped with antibacterial peptide |
| CN114904041A (en) * | 2022-05-31 | 2022-08-16 | 山东大学 | Medical dressing based on humifuse euphorbia herb extract and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110141676A (en) * | 2019-05-27 | 2019-08-20 | 南通大学附属医院 | The medical suture and preparation method thereof of medicine controlled releasing effect can be characterized |
| CN111139551A (en) * | 2019-12-31 | 2020-05-12 | 青岛大学 | Preparation method of skin-core structure intelligent color-changing calcium alginate fiber |
| CN113244438A (en) * | 2021-04-29 | 2021-08-13 | 五邑大学 | Preparation method of three-dimensional functional medical dressing for diabetic foot ulcer |
| CN113350562A (en) * | 2021-06-04 | 2021-09-07 | 南京鼓楼医院 | Preparation method and application of gellan gum bionic microfiber doped with antibacterial peptide |
| CN114904041A (en) * | 2022-05-31 | 2022-08-16 | 山东大学 | Medical dressing based on humifuse euphorbia herb extract and preparation method thereof |
| CN114904041B (en) * | 2022-05-31 | 2023-02-28 | 山东大学 | Medical dressing based on humifuse euphorbia herb extract and preparation method thereof |
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