CN109381494A - A kind of method for breaking trachytectum of glossy ganoderma - Google Patents
A kind of method for breaking trachytectum of glossy ganoderma Download PDFInfo
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- CN109381494A CN109381494A CN201811440936.0A CN201811440936A CN109381494A CN 109381494 A CN109381494 A CN 109381494A CN 201811440936 A CN201811440936 A CN 201811440936A CN 109381494 A CN109381494 A CN 109381494A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
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- A61K2236/10—Preparation or pretreatment of starting material
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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Abstract
The invention discloses a kind of method for breaking trachytectum of glossy ganoderma, this method comprises: crushing, oscillation, enzymatic hydrolysis, ultrasonication, drying, ultramicro grinding, freeze-drying, ultrasonication process.Enzymolysis process, ultrasonication process, ultramicro grinding process, refrigerating process have a huge impact sporoderm-broken rate in this method, and also the preliminary decomposition of cell wall has a significant impact the oscillatory process being added after wall breaking enzyme, the step of later period can just be made, plays maximum effect, only through the invention in each step combination, sporoderm-broken rate can just be effectively improved, so that sporoderm-broken rate is reached 99.8%, while reducing the loss of effective component.
Description
Technical field
The invention belongs to Reishi sporule processing technique fields, and in particular to a kind of method for breaking trachytectum of glossy ganoderma.
Background technique
The wall-breaking method of existing Reishi sporule has biologic enzymolysis method, chemical method, physical method etc., but due to lucidum spore powder
Stable effective ingredients it is poor, existing wall breaking technology in order to guarantee the effective component of lucidum spore powder not by broken to the greatest extent
It is bad, and there are sporoderm-broken rate is lower, and it is difficult complete broken wall, effective component is lost the problems such as serious in Reishi sporule.
Summary of the invention
For above-mentioned deficiency in the prior art, the present invention provides a kind of method for breaking trachytectum of glossy ganoderma, can effectively solve
Sporoderm-broken rate existing for existing wall-breaking method is lower, and effective component is lost serious problem in Reishi sporule.
To achieve the above object, the technical solution adopted by the present invention to solve the technical problems is:
A kind of method for breaking trachytectum of glossy ganoderma, comprising the following steps:
(1) it crushes: Reishi sporule is crushed, cross 60-80 mesh, obtain lucidum spore powder；
(2) it vibrates: being by weight that 1:2-4:0.01-0.02 is mixed by lucidum spore powder, distilled water and lywallzyme,
25-35 DEG C of oscillating reactions 1-2h, obtains oscillation mixed liquor；
(3) it digests: being in mass ratio to be digested after 1:0.3-0.8 is mixed by oscillation mixed liquor and mixed enzyme solution, digest
Temperature is 40-50 DEG C, and enzymolysis time 3-4h obtains enzymolysis liquid；Wherein mixed enzyme solution includes cellulase, hemicellulase and fruit
Glue enzyme, the concentration of cellulase, hemicellulase and pectase are respectively 0.5-0.8g/L, 0.3-0.6g/L, 0.4-0.7g/L；
(4) ultrasonication: enzymolysis liquid is filtered, and removes filtrate, by weight with cellulase solution by obtained solid
For 1:5-8 mixing, ultrasonication is then carried out, the power of ultrasonic wave is 250-350W, frequency 20-25KHz, handles the time
For 30-40min, ultrasonication liquid is obtained；Wherein, the concentration of cellulase solution is 0.6-0.8g/L；
(5) dry: by ultrasonication liquid, filtering removes filtrate, obtained solid is dried in vacuo, drying temperature
It is 45-60 DEG C, dry 1-2h；
(6) it pulverizes: dried object obtained by step (5), powdered cellulose and Compritol 888 ATO is mixed, pulverize
To average grain diameter to 2 μm or less；Wherein, the mass ratio of dried object, powdered cellulose and Compritol 888 ATO is 100-200:
0.3-0.5:0.1-0.2；
(7) it is freeze-dried: the substance after ultramicro grinding being kept into 20-30min under the conditions of -20~-30 DEG C, is then existed
Temperature is risen into 10-20 DEG C of holding 10-20min in 5min, then temperature is risen into 30-40 DEG C of holding 10-20min in 5min；
(8) by the substance after freeze-drying in 40-60 DEG C of ultrasonic treatment 30-40min, broken wall ultrasonication: is made
Lucidum spore powder.
It further, is by weight that 1:3:0.015 is mixed by lucidum spore powder, distilled water and lywallzyme in step (2),
In 30 DEG C of oscillating reactions 2h.
Further, the mass ratio of oscillation mixed liquor and mixed enzyme solution is 1:0.5 in step (3), and hydrolysis temperature is 45 DEG C,
Enzymolysis time is 4h.
Further, the concentration of step (3) cellulase, hemicellulase and pectase is respectively 0.6g/L, 0.5g/
L、0.6g/L。
Further, by weight it is that 1:6 is mixed by obtained solid and cellulase solution in step (4), then carries out
Ultrasonication, the power of ultrasonic wave are 300W, and frequency 22KHz, the processing time is 30min.
Further, the concentration of step (4) cellulase solution is 0.6g/L.
Further, drying temperature is 50 DEG C in step (5), dry 1h.
Further, the mass ratio of dried object, powdered cellulose and Compritol 888 ATO is 150:0.35 in step (6):
0.15。
Further, the substance after ultramicro grinding is kept into 30min under the conditions of -25 DEG C in step (7), then in 5min
It is interior that temperature is risen into 20 DEG C of holding 15min, then temperature is risen into 40 DEG C of holding 10min in 5min.
Further, in step (8) by the substance after freeze-drying in 50 DEG C of ultrasonic treatment 30min.
Method for breaking trachytectum of glossy ganoderma provided by the invention, has the advantages that
The present invention decomposes the ingredient in Reishi sporule cell wall with lywallzyme first, so that the dissolution for reducing cell wall is difficult
Degree, digests cell wall in conjunction with cellulase, hemicellulase and pectase, can damage cells the structure of wall, make it
The collapsing, dissolution, loose for generating part, improves its sporoderm-broken rate.
After enzymatic hydrolysis, Reishi sporule cell wall has generated part collapsing, thin when that can further speed up with ultrasonication
Cell wall is disintegrated, especially in ultrasonication combine enzymatic treatment, shell-broken effect more preferably so that in Reishi sporule cell wall
Molecular structure is looser, and aperture is bigger, is more advantageous to later period complete broken wall treatment, and not will lead to effective component in Reishi sporule
Loss.
Substance carries out Ultramicro-powder after enzyme auxiliary ultrasonic is to the processing of Reishi sporule cell wall, then by treated
It is broken, external force can be generated to Reishi sporule cell wall in crushing process, can destroy the molecular structure of Reishi sporule cell wall, work as addition
After cellulose and Compritol 888 ATO, crush efficiency can be significantly improved, the sporoderm-broken rate of cell wall greatly improves, when in conjunction with low temperature
Freezing processing further improves sporoderm-broken rate in this way, while also effectively remaining the nutritional ingredient of lucidum spore powder, together
The specific heating mode of Shi Caiyong, when so that the later period carrying out ultrasonication again, sporoderm-broken rate improves again, also improves broken wall
Efficiency.
Each step is all linked with one another in the present invention, it is high just to make sporoderm-broken rate, and not will lead to effective component in Reishi sporule
It is lost.
Specific embodiment
Embodiment 1
A kind of method for breaking trachytectum of glossy ganoderma, comprising the following steps:
(1) it crushes: Reishi sporule is crushed, cross 60-80 mesh, obtain lucidum spore powder；
(2) it vibrates: being by weight that 1:2:0.01 is mixed by lucidum spore powder, distilled water and lywallzyme, vibrated at 25 DEG C
2h is reacted, oscillation mixed liquor is obtained；
(3) it digests: being in mass ratio to be digested after 1:0.3 is mixed by oscillation mixed liquor and mixed enzyme solution, hydrolysis temperature
It is 40 DEG C, enzymolysis time 4h obtains enzymolysis liquid；Wherein mixed enzyme solution includes cellulase, hemicellulase and pectase, fiber
The concentration of plain enzyme, hemicellulase and pectase is respectively 0.5g/L, 0.3g/L, 0.4g/L；
(4) ultrasonication: enzymolysis liquid is filtered, and removes filtrate, by weight with cellulase solution by obtained solid
For 1:5 mixing, ultrasonication is then carried out, the power of ultrasonic wave is 250W, and frequency 20KHz, the processing time is 40min,
Obtain ultrasonication liquid；Wherein, the concentration of cellulase solution is 0.6g/L；
(5) dry: by ultrasonication liquid, filtering removes filtrate, obtained solid is dried in vacuo, drying temperature
It is 45 DEG C, dry 2h；
(6) it pulverizes: dried object obtained by step (5), powdered cellulose and Compritol 888 ATO is mixed, pulverize
To average grain diameter to 2 μm or less；Wherein, the mass ratio of dried object, powdered cellulose and Compritol 888 ATO is 100:0.3:
0.1；
(7) it is freeze-dried: the substance after ultramicro grinding is kept into 25min under the conditions of -20 DEG C, it then will be warm in 5min
Degree rises to 10 DEG C of holding 20min, then temperature is risen to 30 DEG C of holding 20min in 5min；
(8) by the substance after freeze-drying in 40 DEG C of ultrasonic treatment 40min, the ganoderma lucidum spore of broken wall ultrasonication: is made
Sub- powder.
Embodiment 2
A kind of method for breaking trachytectum of glossy ganoderma, comprising the following steps:
(1) it crushes: Reishi sporule is crushed, cross 60-80 mesh, obtain lucidum spore powder；
(2) it vibrates: being by weight that 1:4:0.02 is mixed by lucidum spore powder, distilled water and lywallzyme, vibrated at 35 DEG C
1h is reacted, oscillation mixed liquor is obtained；
(3) it digests: being in mass ratio to be digested after 1:0.8 is mixed by oscillation mixed liquor and mixed enzyme solution, hydrolysis temperature
It is 50 DEG C, enzymolysis time 3h obtains enzymolysis liquid；Wherein mixed enzyme solution includes cellulase, hemicellulase and pectase, fiber
The concentration of plain enzyme, hemicellulase and pectase is respectively 0.8g/L, 0.6g/L, 0.7g/L；
(4) ultrasonication: enzymolysis liquid is filtered, and removes filtrate, by weight with cellulase solution by obtained solid
For 1:8 mixing, ultrasonication is then carried out, the power of ultrasonic wave is 350W, and frequency 25KHz, the processing time is 30min,
Obtain ultrasonication liquid；Wherein, the concentration of cellulase solution is 0.8g/L；
(5) dry: by ultrasonication liquid, filtering removes filtrate, obtained solid is dried in vacuo, drying temperature
It is 60 DEG C, dry 1h；
(6) it pulverizes: dried object obtained by step (5), powdered cellulose and Compritol 888 ATO is mixed, pulverize
To average grain diameter to 2 μm or less；Wherein, the mass ratio of dried object, powdered cellulose and Compritol 888 ATO is 200:0.5:
0.2；
(7) it is freeze-dried: the substance after ultramicro grinding is kept into 20min under the conditions of -30 DEG C, it then will be warm in 5min
Degree rises to 10 DEG C of holding 20min, then temperature is risen to 30 DEG C of holding 20min in 5min；
(8) by the substance after freeze-drying in 60 DEG C of ultrasonic treatment 30min, the ganoderma lucidum spore of broken wall ultrasonication: is made
Sub- powder.
Embodiment 3
A kind of method for breaking trachytectum of glossy ganoderma, comprising the following steps:
(1) it crushes: Reishi sporule is crushed, cross 60-80 mesh, obtain lucidum spore powder；
(2) it vibrates: being by weight that 1:3:0.15 is mixed by lucidum spore powder, distilled water and lywallzyme, vibrated at 30 DEG C
2h is reacted, oscillation mixed liquor is obtained；
(3) it digests: being in mass ratio to be digested after 1:0.5 is mixed by oscillation mixed liquor and mixed enzyme solution, hydrolysis temperature
It is 45 DEG C, enzymolysis time 4h obtains enzymolysis liquid；Wherein mixed enzyme solution includes cellulase, hemicellulase and pectase, fiber
The concentration of plain enzyme, hemicellulase and pectase is respectively 0.6g/L, 0.5g/L, 0.6g/L；
(4) ultrasonication: enzymolysis liquid is filtered, and removes filtrate, by weight with cellulase solution by obtained solid
For 1:6 mixing, ultrasonication is then carried out, the power of ultrasonic wave is 300W, and frequency 22KHz, the processing time is 30min,
Obtain ultrasonication liquid；Wherein, the concentration of cellulase solution is 0.6g/L；
(5) dry: by ultrasonication liquid, filtering removes filtrate, obtained solid is dried in vacuo, drying temperature
It is 50 DEG C, dry 1h；
(6) it pulverizes: dried object obtained by step (5), powdered cellulose and Compritol 888 ATO is mixed, pulverize
To average grain diameter to 2 μm or less；Wherein, the mass ratio of dried object, powdered cellulose and Compritol 888 ATO is 150:0.35:
0.15；
(7) it is freeze-dried: the substance after ultramicro grinding is kept into 30min under the conditions of -25 DEG C, it then will be warm in 5min
Degree rises to 20 DEG C of holding 15min, then temperature is risen to 40 DEG C of holding 10min in 5min；
(8) by the substance after freeze-drying in 50 DEG C of ultrasonic treatment 30min, the ganoderma lucidum spore of broken wall ultrasonication: is made
Sub- powder.
Comparative example 1
Comparative example 1 compared with Example 3, distinguishes place are as follows: deletion procedure (2), directly by smashed lucidum spore powder
It mixes and is digested with mixed enzyme solution, remaining is same as Example 3.
Comparative example 2
Comparative example 2 compared with Example 3, distinguishes place are as follows: lacks step (3), directly filters oscillation mixed liquor and carry out
Ultrasonication, remaining is same as Example 3.
Comparative example 3
Comparative example 3 compared with Example 3, distinguishes place are as follows: lack step (4), remaining is same as Example 3.
Comparative example 4
Comparative example 4 compared with Example 3, distinguishes place are as follows: lack step (6), remaining is same as Example 3.
Comparative example 5
Comparative example 5 compared with Example 3, distinguishes place are as follows: lack step (7), remaining is same as Example 3.
Comparative example 6
Comparative example 6 compared with Example 3, difference place are as follows: lack step (3) and step (4), remaining and 3 phase of embodiment
Together.
Comparative example 7
Comparative example 7 compared with Example 3, difference place are as follows: lack step (4) and step (6), remaining and 3 phase of embodiment
Together.
The Reishi sporule of 10 parts of equivalent is taken to pass through embodiment 1-3 and comparative example 1-7 method progress broken wall treatment respectively, to broken
Wall rate, amino acid, fatty acid are detected in the lucidum spore powder after broken wall, and testing result is as shown in table 1.
The sporoderm-broken rate and amino acid, content of fatty acid of each embodiment of table 1
As shown in Table 1, enzymolysis process, ultrasonication process, ultramicro grinding process, refrigerating process generate sporoderm-broken rate
Very big influence, and also the preliminary decomposition of cell wall has a significant impact the oscillatory process being added after wall breaking enzyme, can just make the later period
Step plays maximum effect, only through the invention in each step combination, can just effectively improve sporoderm-broken rate, make sporoderm-broken rate
Reach 99.8%, while reducing the loss of effective component.
Claims (10)
1. a kind of method for breaking trachytectum of glossy ganoderma, which comprises the following steps:
(1) it crushes: Reishi sporule is crushed, cross 60-80 mesh, obtain lucidum spore powder；
(2) it vibrates: being by weight that 1:2-4:0.01-0.02 is mixed by lucidum spore powder, distilled water and lywallzyme, in 25-35
DEG C oscillating reactions 1-2h, obtains oscillation mixed liquor；
(3) it digests: being in mass ratio to be digested after 1:0.3-0.8 is mixed by oscillation mixed liquor and mixed enzyme solution, hydrolysis temperature
It is 40-50 DEG C, enzymolysis time 3-4h obtains enzymolysis liquid；Wherein mixed enzyme solution includes cellulase, hemicellulase and pectin
Enzyme, the concentration of cellulase, hemicellulase and pectase are respectively 0.5-0.8g/L, 0.3-0.6g/L, 0.4-0.7g/L；
(4) ultrasonication: enzymolysis liquid is filtered, and removes filtrate, is by weight 1 by obtained solid and cellulase solution:
5-8 is mixed, and then carries out ultrasonication, and the power of ultrasonic wave is 250-350W, frequency 20-25KHz, and the processing time is
30-40min obtains ultrasonication liquid；Wherein, the concentration of cellulase solution is 0.6-0.8g/L；
(5) dry: by ultrasonication liquid, filtering removes filtrate, obtained solid is dried in vacuo, drying temperature 45-
60 DEG C, dry 1-2h；
(6) it pulverizes: dried object obtained by step (5), powdered cellulose and Compritol 888 ATO is mixed, pulverize to flat
Equal partial size is to 2 μm or less；Wherein, the mass ratio of dried object, powdered cellulose and Compritol 888 ATO is 100-200:0.3-
0.5:0.1-0.2；
(7) it is freeze-dried: the substance after ultramicro grinding being kept into 20-30min under the conditions of -20~-30 DEG C, then in 5min
Temperature is risen into 10-20 DEG C of holding 10-20min, then temperature is risen into 30-40 DEG C of holding 10-20min in 5min；
(8) by the substance after freeze-drying in 40-60 DEG C of ultrasonic treatment 30-40min, the ganoderma lucidum of broken wall ultrasonication: is made
Conidia powder.
2. method for breaking trachytectum of glossy ganoderma according to claim 1, which is characterized in that by lucidum spore powder, steaming in step (2)
Distilled water and lywallzyme are 1:3:0.015 mixing by weight, in 30 DEG C of oscillating reactions 2h.
3. method for breaking trachytectum of glossy ganoderma according to claim 1, which is characterized in that vibrate mixed liquor in step (3) and mix
The mass ratio of synthase liquid is 1:0.5, and hydrolysis temperature is 45 DEG C, enzymolysis time 4h.
4. method for breaking trachytectum of glossy ganoderma according to claim 1, which is characterized in that step (3) cellulase, hemicellulose
The concentration of plain enzyme and pectase is respectively 0.6g/L, 0.5g/L, 0.6g/L.
5. method for breaking trachytectum of glossy ganoderma according to claim 1, which is characterized in that by obtained solid and fibre in step (4)
Tie up plain enzyme solutions is that 1:6 is mixed by weight, then carries out ultrasonication, the power of ultrasonic wave is 300W, and frequency is
22KHz, processing time are 30min.
6. method for breaking trachytectum of glossy ganoderma according to claim 1, which is characterized in that step (4) cellulase solution
Concentration is 0.6g/L.
7. method for breaking trachytectum of glossy ganoderma according to claim 1, which is characterized in that drying temperature is 50 DEG C in step (5),
Dry 1h.
8. method for breaking trachytectum of glossy ganoderma according to claim 1, which is characterized in that dried object, flour in step (6)
The mass ratio of element and Compritol 888 ATO is 150:0.35:0.15.
9. method for breaking trachytectum of glossy ganoderma according to claim 1, which is characterized in that will be after ultramicro grinding in step (7)
Substance keeps 30min under the conditions of -25 DEG C, temperature is then risen to 20 DEG C of holding 15min in 5min, then will be warm in 5min
Degree rises to 40 DEG C of holding 10min.
10. method for breaking trachytectum of glossy ganoderma according to claim 1, which is characterized in that will be after freeze-drying in step (8)
Substance is in 50 DEG C of ultrasonic treatment 30min.
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| CN110339227A (en) * | 2019-07-05 | 2019-10-18 | 安徽省泽华国药饮片有限公司 | A kind of preparation method of fresh Chinese herbal medicine freezing broken wall |
| CN115444776A (en) * | 2022-09-23 | 2022-12-09 | 苏州猫尔科技有限公司 | Hair care and hair growth composition and application thereof |
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| CN110339227A (en) * | 2019-07-05 | 2019-10-18 | 安徽省泽华国药饮片有限公司 | A kind of preparation method of fresh Chinese herbal medicine freezing broken wall |
| CN115444776A (en) * | 2022-09-23 | 2022-12-09 | 苏州猫尔科技有限公司 | Hair care and hair growth composition and application thereof |
| CN115444776B (en) * | 2022-09-23 | 2023-06-09 | 苏州猫尔科技有限公司 | Hair care and hair growth composition and application thereof |
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