CN109371091A - A kind of Chinese yam peptide and its extracting method - Google Patents
A kind of Chinese yam peptide and its extracting method Download PDFInfo
- Publication number
- CN109371091A CN109371091A CN201811599594.7A CN201811599594A CN109371091A CN 109371091 A CN109371091 A CN 109371091A CN 201811599594 A CN201811599594 A CN 201811599594A CN 109371091 A CN109371091 A CN 109371091A
- Authority
- CN
- China
- Prior art keywords
- chinese yam
- peptide
- extracting method
- desugar
- enzymolysis liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to Chinese yam deep process technology field, a kind of Chinese yam peptide and its extracting method are specifically disclosed.The extracting method at least includes the following steps: Chinese yam step a, being added water boiling, is cooled down, amylase and carbohydrase is then added, desugar is carried out and digests to obtain Chinese yam gruel；Step b, the Chinese yam gruel is subjected to centrifugation desugar, it is rotten obtains desugar Chinese yam；Step c, purified water is added in desugar Chinese yam gruel, adds pronase and is digested, enzymatic hydrolysis terminates to carry out enzyme deactivation, obtains the first enzymolysis liquid；Step d, the first enzymolysis liquid is cooled down, neutral proteinase is added and flavor protease is digested, enzymatic hydrolysis terminates to carry out enzyme deactivation, obtains the second enzymolysis liquid；Step e, the Chinese yam peptide for the second enzymolysis liquid being filtered, organizing membrane separation technique, being dried to obtain different molecular weight.Extracting method provided by the invention, Chinese yam peptide activity is high, and molecular weight is small, and solubility high 100% is dissolved in water.
Description
Technical field
The present invention relates to Chinese yam deep process technology field more particularly to a kind of Chinese yam peptide and its extracting methods.
Background technique
Chinese yam, i.e. Chinese yam, alias RHIIZOMA DIOSCOREAE from Henan of China, Chinese yam rhizome, native potato, sweet potato, sweet potato, jade prolong.Herbaceos perennial, vines
Raw, often band purple, root tuber cylinder, leaf are now distributed in China North China, northwest and the Yangtze river basin to life, elliptical or oval shape
Each provinces and regions in Jiangxi.
It is rich in a variety of nutriments in Chinese yam stem tuber, there is strengthening the spleen and stomach, tonifying lung kidney, tonifying middle-Jiao and Qi, invigorating the spleen qi-restoratives, benefit of reinforcing the kidney
The effects of essence, invigorating heart are calmed the nerves.Mucilaginous substance, zymigen of the polysaccharide protein ingredient especially contained in fresh tuber etc., can be pre-
Anti- angiocarpy fat deposition, facilitates the digestion and absorption of stomach and intestine.
However, polysaccharide protein molecule is larger, human body is not easy to absorb, and easily causes allergic reaction.The study found that peptide
Biological value and nutritive value are higher than protein molecule and free amino acid.Active peptide also has a variety of body metabolisms and physiology
Regulatory function has the effects of promotion immune, hormone control, antibacterial, antiviral, lowering blood pressure and blood fat, and edible safety is high.
Research for peptide is always the forward position that biochemistry aspect is studied, and the protein in different material is decomposed into peptide, function
Can be also different, Chinese yam peptide also has its uniqueness.Chinese yam peptide is mainly prepared using enzymatic isolation method at present, but preparation process is multiple
It is miscellaneous, and the purity of Chinese yam peptide is low, molecular weight is high and recovery rate is low, greatly limits Chinese yam peptide answering in food or health food
With.
Summary of the invention
For the Chinese yam peptide of existing enzymatic isolation method preparation purity is low, molecular weight is high and recovery rate is low problem, the present invention mentions
For a kind of extracting method of Chinese yam peptide.
And the extracting method of above-mentioned Chinese yam peptide is extracted to obtain Chinese yam peptide.
To achieve the above object of the invention, the embodiment of the present invention uses the following technical solution:
A kind of extracting method of Chinese yam peptide, the extracting method at least include the following steps:
Step a, Chinese yam is added into water boiling, cooled down, amylase then is added and carbohydrase is digested；
Step b, the enzymolysis liquid for obtaining step a carries out centrifugation desugar, and it is rotten to obtain desugar Chinese yam；
Step c, purified water is added in desugar Chinese yam gruel, is warming up to 45-50 DEG C, add pronase and carry out enzyme
Solution, enzymatic hydrolysis terminate to carry out enzyme deactivation, obtain the first enzymolysis liquid, wherein enzymatic hydrolysis condition are as follows: enzymatic hydrolysis pH value is 7.8-8, and enzymolysis time is
2-3h；
Step d, the first enzymolysis liquid is cooled to 50-55 DEG C, neutral proteinase is added and flavor protease is digested, enzyme
Solution terminates to carry out enzyme deactivation, obtains the second enzymolysis liquid, wherein enzymatic hydrolysis condition are as follows: enzymatic hydrolysis pH value is 6-7, enzymolysis time 3-4h；
Step e, the second enzymolysis liquid obtains Chinese yam peptide after separation, purifying and drying.Compared with the existing technology, the present invention mentions
The extracting method of the Chinese yam peptide of confession has the advantage that
(1) present invention takes amylase and carbohydrase to combine and carries out double enzyme desugar enzymatic hydrolysis, is by the Starch Conversion in Chinese yam
It is not sticky that oligosaccharide, carbohydrase make liquid glucose further liquefy, and makes enzymolysis liquid be easier to filter, reduces filtration time, be conducive to mention
High product yield.
(2) protein is digested using two-step method, protein is digested using pronase first, reduced
The relative polymerization degree of macro-molecular protein in system, catalytic proteins part peptide fragment are broken, sufficiently will be in desugar Chinese yam gruel
Mucin digested, reduce the viscosity of enzymolysis liquid, be conducive to enzyme digestion reaction progress, followed by use neutral proteinase and wind
Taste protease is digested, and using neutral proteinase as restriction endonuclease, protein is allow to be hydrolyzed into peptide fragment, and will inside peptide chain
Polypeptide is hydrolyzed into small fragment, using flavor protease as excision enzyme, acts on the peptide bond of peptide chain end, reduces the length of peptide chain, drop
The ratio of hydrophobic amino acid in low enzymolysis liquid further increases degree of hydrolysis and improves flavor.With pronase, neutral proteinase
Chinese yam protain is hydrolyzed with the mixed enzyme of flavor protease compounding, plays the hydrolysis properties of different enzymes, is had complementary advantages, is realized
The multidigit point of Chinese yam protain is decomposed, under the premise of improving Chinese yam hydrolase polypeptide degree, and can prevent from excessively hydrolyzing and generate a large amount of ammonia
Base acid, to improve the recovery rate of Chinese yam peptide, and hydrophobic amino acid bring bitter taste can be removed, improve product mouthfeel and
Flavor.
(3) Chinese yam protain is digested with the mixed enzyme that pronase, neutral proteinase and flavor protease compound,
The molecular weight of product Chinese yam peptide is small, and narrowly distributing, greatly improves the application of Chinese yam peptide.
(4) present invention takes filtering and group membrane separation technique to combine, and simple process operates, at normal temperature without phase
Change and does not generate pollution in production process；Salts substances of the molecular weight less than 100 dalton not only can be preferably sloughed,
So that Chinese yam peptide is able to maintain better purity, and more preferably make filtrate concentration, save subsequent handling concentration time, reduce energy consumption, obtains
To the Chinese yam peptide of different molecular weight, more it is able to satisfy drug and health food is directed to the particular/special requirement of different syndromes.
(5) extracting method provided by the invention it is with short production cycle, it is at low cost, do not generate any poisonous and harmful substance, safety,
It has no toxic side effect, product is in good taste, free from extraneous odour, and no bitter taste can be widely applied to the fields such as food, health care product, drug；Simultaneously
Reaction condition is mild, easy to operate, further improves the safety and utility value of product.
And specifically, it is preferable to ground, in step c, the additional amount of the pronase is the 0.5-1% of the Chinese yam quality.
Preferably, in step d, the neutral proteinase is formed by fermentation of bacillus subtilis, and additional amount is the mountain
The 1-1.5% of medicine quality, the additional amount of the flavor protease are the 0.3-0.5% of the Chinese yam quality.
Preferably, in step e, second enzymolysis liquid using three-phase centrifuge be centrifuged, obtained centrifugate by nanofiltration,
The Chinese yam peptide of different molecular weight is obtained after concentration and drying.
The present invention uses three phase centrifugation technology, removes insoluble matter in enzymolysis liquid, is that subsequent group of membrane separation technique uses
The nanofiltration membrane of different pore size separates the concentrate of different molecular weight, and the Chinese yam peptide for being dried to obtain different molecular weight provides base
Plinth.
Preferably, the collecting and filtering apparatus that the centrifugate successively passes through that nanofiltration membrane is 1 nanometer, 0.2 nanometer and 0.5 nanometer separates,
The liquid between 1-0.2 nanometers and 0.2-0.5 nanometers is taken, it is post-treated to respectively obtain 500-1000 dalton and the road 180-500
The Chinese yam peptide that you pause.
It is filtered using the nanofiltration membrane of 1 nanometer, 0.2 nanometer and 0.5 nanometer three kinds of different pore sizes, three combines, no
But salts substances of the molecular weight less than 100 dalton can be preferably sloughed, so that Chinese yam peptide is able to maintain better purity, and more preferable
Make filtrate concentration, saves subsequent handling concentration time, reduces energy consumption.
Preferably, in step a, the amylase is that solution fermentation of bacillus forms, and additional amount is the Chinese yam quality
1-1.5%.
Preferably, in step a, the carbohydrase is glucoamylase, ferments to obtain by aspergillus oryzae, and additional amount is institute
State the 0.5-0.8% of Chinese yam quality.
Preferably, in step a, the desugar enzymatic hydrolysis condition are as follows: hydrolysis temperature are as follows: 55-58 DEG C, enzymolysis time 2-3h.
Preferably, in step a, Chinese yam is crushed, be added 5-6 times of Chinese yam quality of the purified water stirring, 90 DEG C-
Under the conditions of 95 DEG C, boiling 20-25min.
Preferably, in step c, the additional amount of the purified water is 3-4 times of the Chinese yam quality.
Preferably, the condition of the enzyme deactivation are as follows: enzyme-removal temperature is 90-95 DEG C, and the enzyme deactivation time is 10-15min.
High-temperature heat treatment can sufficiently knock out the residual activity of enzyme and realize the Maillard reaction improvement flavor of appropriateness, and
Have microorganism extremely low after heat treatment, the performance of storage characteristics enhancing.
Preferably, the drying mode is spray drying.
Further, the present invention also provides a kind of Chinese yam peptides, extract to obtain using the extracting method of above-mentioned Chinese yam peptide.
It is up to 74% or more using the recovery rate of Chinese yam peptide obtained in the second enzymolysis liquid of extracting method provided by the invention,
And obtained Chinese yam peptide molecular weight is small, and narrowly distributing, peptide molecular weight is that the peptide content of 180-500 dalton reaches 52% or more,
Peptide molecular weight is the peptide content of 500-1000 dalton 23% or more.Second enzymolysis liquid is respectively obtained after separating-purifying
The Chinese yam peptide of 500-1000 dalton and 180-500 dalton, is able to satisfy in medication chemistry and food or field of health care food
Use for different demands to Chinese yam peptide.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1
The present invention is that embodiment provides a kind of extracting method of Chinese yam peptide, the extracting method the following steps are included:
Step a, it by fresh yam 1000g, is blended by meat grinder, 5000mL purified water is added and stirs evenly, in 90 DEG C of items
Under part, boiling 20min；Then 55 DEG C are cooled to, 10g amylase and 5g carbohydrase are separately added into, temperature is maintained at 55 DEG C, stirring
Enzymatic hydrolysis 2h obtains Chinese yam gruel；
Step b, Chinese yam gruel decanter centrifuge being centrifuged desugar, liquid phase is further processed to obtain yam oligosaccharide,
It leaves and takes the gruel of desugar Chinese yam and carries out centrifugation desugar, it is rotten to obtain desugar Chinese yam；
Step c, the Chinese yam after desugar is rotten, 3000mL purified water is added, is warming up to 50 DEG C, adds 10g strepto- egg
White enzyme is digested, and 90 DEG C are warming up to after enzymatic hydrolysis, is kept 10min enzyme deactivation, is obtained the first enzymolysis liquid, wherein enzymatic hydrolysis condition
Are as follows: enzymatic hydrolysis pH value is 7.8, enzymolysis time 3h；
Step d, the first enzymolysis liquid is cooled to 50 DEG C, 10g neutral proteinase and 3g flavor protease is added, in pH value
Under conditions of 6, stirring enzymatic hydrolysis 3h then heats to 90 DEG C, keeps 15min enzyme deactivation, the second enzymolysis liquid；
Step e, it will be filtered after the second enzymolysis liquid with three-phase centrifuge, it is 1 nanometer, 0.2 that centrifugate, which successively passes through nanofiltration membrane,
Nanometer is separated with 0.5 nanometer of collecting and filtering apparatus, is taken the liquid between 1-0.2 nanometers and 0.2-0.5 nanometers, is used dual-effect concentrator respectively
Further concentration, after spray drying, respectively obtains the Chinese yam peptide of 500-1000 dalton and 180-500 dalton, wherein 500-
The Chinese yam peptide of 1000 dalton can be used for health food, and the Chinese yam peptide of 180-500 dalton can be used for drug.
Embodiment 2
The present invention is that embodiment provides a kind of extracting method of Chinese yam peptide, the extracting method the following steps are included:
Step a, it by fresh yam 10Kg, is blended by meat grinder, 55L purified water is added and stirs evenly, under the conditions of 92 DEG C,
Boiling 23min；Then 56 DEG C are cooled to, 100g amylase and 50g carbohydrase are separately added into, temperature is maintained at 56 DEG C, stirs enzyme
Solution 2.5h obtains Chinese yam gruel；
Step b, Chinese yam gruel decanter centrifuge being centrifuged desugar, liquid phase is further processed to obtain yam oligosaccharide,
It leaves and takes the gruel of desugar Chinese yam and carries out centrifugation desugar, it is rotten to obtain desugar Chinese yam；
Step c, the Chinese yam after desugar is rotten, 35L purified water is added, is warming up to 45 DEG C, adds 50g pronase
It is digested, 95 DEG C is warming up to after enzymatic hydrolysis, kept 10min enzyme deactivation, obtain the first enzymolysis liquid, wherein enzymatic hydrolysis condition are as follows: enzyme
Solving pH value is 8, enzymolysis time 2.5h；
Step d, the first enzymolysis liquid is cooled to 53 DEG C, 120g neutral proteinase and 35g flavor protease is added, in pH
Under conditions of value is 6, stirring enzymatic hydrolysis 3.5h then heats to 93 DEG C, keeps 13min enzyme deactivation, the second enzymolysis liquid；
Step e, it will be filtered after the second enzymolysis liquid with three-phase centrifuge, it is 1 nanometer, 0.2 that centrifugate, which successively passes through nanofiltration membrane,
Nanometer is separated with 0.5 nanometer of collecting and filtering apparatus, takes the liquid between 1-0.2 nanometers and 0.2-0.5 nanometers, with dual-effect concentrator into one
The Chinese yam peptide of 500-1000 dalton and 180-500 dalton is arrived in step concentration after spray drying.
Embodiment 3
The present invention is that embodiment provides a kind of extracting method of Chinese yam peptide, the extracting method the following steps are included:
Step a, it by fresh yam 100Kg, is blended by meat grinder, 600L purified water is added and stirs evenly, in 95 DEG C of conditions
Under, boiling 25min；Then 58 DEG C are cooled to, 1Kg amylase and 500g carbohydrase are separately added into, temperature is maintained at 58 DEG C, stirring
Enzymatic hydrolysis 3h obtains Chinese yam gruel；
Step b, Chinese yam gruel decanter centrifuge being centrifuged desugar, liquid phase is further processed to obtain yam oligosaccharide,
It leaves and takes the gruel of desugar Chinese yam and carries out centrifugation desugar, it is rotten to obtain desugar Chinese yam；
Step c, the Chinese yam after desugar is rotten, 400L purified water is added, is warming up to 42 DEG C, adds 700g pronase
Enzyme is digested, and 93 DEG C are warming up to after enzymatic hydrolysis, is kept 13min enzyme deactivation, is obtained the first enzymolysis liquid, wherein enzymatic hydrolysis condition are as follows:
Digesting pH value is 7.9, enzymolysis time 2h；
Step d, the first enzymolysis liquid is cooled to 55 DEG C, adds 1.5Kg neutral proteinase and 400g flavor protease,
Under conditions of pH value is 7, stirring enzymatic hydrolysis 4h then heats to 95 DEG C, keeps 15min enzyme deactivation, the second enzymolysis liquid；
Step e, it will be filtered after the second enzymolysis liquid with three-phase centrifuge, it is 1 nanometer, 0.2 that centrifugate, which successively passes through nanofiltration membrane,
Nanometer is separated with 0.5 nanometer of collecting and filtering apparatus, is taken the liquid between 1-0.2 nanometers and 0.2-0.5 nanometers, is used dual-effect concentrator respectively
Further concentration, after spray drying, respectively obtains the Chinese yam peptide of 500-1000 dalton and 180-500 dalton, wherein 500-
The Chinese yam peptide of 1000 dalton can be used for health food, and the Chinese yam peptide of 180-500 dalton can be used for drug.
Comparative example 1
This comparative example provides a kind of extracting method of Chinese yam peptide, the extracting method the following steps are included:
Step a, it by fresh yam 1000g, is blended by meat grinder, 5000mL purified water is added and stirs evenly, in 90 DEG C of items
Under part, boiling 20min；Then 55 DEG C are cooled to, 10g amylase and 5g carbohydrase are separately added into, temperature is maintained at 55 DEG C, stirring
Enzymatic hydrolysis 2h obtains Chinese yam gruel；
Step b, Chinese yam gruel decanter centrifuge being centrifuged desugar, liquid phase is further processed to obtain yam oligosaccharide,
It leaves and takes the gruel of desugar Chinese yam and carries out centrifugation desugar, it is rotten to obtain desugar Chinese yam；
Step c, the Chinese yam after desugar is rotten, 3000mL purified water is added, is warming up to 50 DEG C, adds 10g stomach cardia
Enzyme is digested, and 90 DEG C are warming up to after enzymatic hydrolysis, is kept 10min enzyme deactivation, is obtained the first enzymolysis liquid, wherein enzymatic hydrolysis condition are as follows:
Digesting pH value is 7.8, enzymolysis time 3h；
Step d, the first enzymolysis liquid is cooled to 50 DEG C, 10g neutral proteinase and 3g flavor protease is added, in pH value
Under conditions of 6, stirring enzymatic hydrolysis 3h then heats to 90 DEG C, keeps 15min enzyme deactivation, the second enzymolysis liquid；
Step e, it will be filtered after the second enzymolysis liquid with three-phase centrifuge, it is 1 nanometer, 0.2 that centrifugate, which successively passes through nanofiltration membrane,
Nanometer is separated with 0.5 nanometer of collecting and filtering apparatus, is taken the liquid between 1-0.2 nanometers and 0.2-0.5 nanometers, is used dual-effect concentrator respectively
Further concentration, after spray drying, respectively obtains the Chinese yam peptide of 500-1000 dalton and 180-500 dalton, wherein 500-
The Chinese yam peptide of 1000 dalton can be used for health food, and the Chinese yam peptide of 180-500 dalton can be used for drug.
Comparative example 2
This comparative example provides a kind of extracting method of Chinese yam peptide, the extracting method the following steps are included:
Step a, it by fresh yam 1000g, is blended by meat grinder, 5000mL purified water is added and stirs evenly, in 90 DEG C of items
Under part, boiling 20min；Then 55 DEG C are cooled to, 10g amylase and 5g carbohydrase are separately added into, temperature is maintained at 55 DEG C, stirring
Enzymatic hydrolysis 2h obtains Chinese yam gruel；
Step b, Chinese yam gruel decanter centrifuge being centrifuged desugar, liquid phase is further processed to obtain yam oligosaccharide,
It leaves and takes the gruel of desugar Chinese yam and carries out centrifugation desugar, it is rotten to obtain desugar Chinese yam；
Step c, the Chinese yam after desugar is rotten, 3000mL purified water is added, is warming up to 50 DEG C, adds 10g strepto- egg
White enzyme is digested, and 90 DEG C are warming up to after enzymatic hydrolysis, is kept 10min enzyme deactivation, is obtained the first enzymolysis liquid, wherein enzymatic hydrolysis condition
Are as follows: enzymatic hydrolysis pH value is 7.8, enzymolysis time 3h；
Step d, the first enzymolysis liquid is cooled to 50 DEG C, 10g neutral proteinase and 3g papain is added, in pH value
Under conditions of 6, stirring enzymatic hydrolysis 3h then heats to 90 DEG C, keeps 15min enzyme deactivation, the second enzymolysis liquid；
Step e, it will be filtered after the second enzymolysis liquid with three-phase centrifuge, it is 1 nanometer, 0.2 that centrifugate, which successively passes through nanofiltration membrane,
Nanometer is separated with 0.5 nanometer of collecting and filtering apparatus, is taken the liquid between 1-0.2 nanometers and 0.2-0.5 nanometers, is used dual-effect concentrator respectively
Further concentration, after spray drying, respectively obtains the Chinese yam peptide of 500-1000 dalton and 180-500 dalton, wherein 500-
The Chinese yam peptide of 1000 dalton can be used for health food, and the Chinese yam peptide of 180-500 dalton can be used for drug.
The characteristic for the Chinese yam peptide that embodiment provides in order to better illustrate the present invention, below by embodiment 1-3 and comparison
The recovery rate of Chinese yam peptide and molecular weight distribution such as the following table 1 institute in the second enzymolysis liquid that step c is obtained in example 1-2 preparation process
Show.
Table 1
Note: recovery rate=Chinese yam peptide quality/Chinese yam protain quality
From table 1 it follows that the Chinese yam peptides extraction rate of extracting method preparation provided by the invention is up to 74% or more, obtain
The Chinese yam peptide molecular weight arrived is small, and narrowly distributing, reaches 52% or more in the peptide content that peptide molecular weight is 180-500 dalton, peptide
Molecular weight is the peptide content of 500-1000 dalton 23% or more.And Chinese yam peptides extraction rate prepared by comparative example is low, and point
Son amount is big, and distribution is wide.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modification, equivalent replacement or improvement etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (9)
1. a kind of extracting method of Chinese yam peptide, it is characterised in that: the extracting method at least includes the following steps:
Step a, Chinese yam is added into water boiling, cooled down, amylase then is added and carbohydrase is digested；
Step b, the enzymolysis liquid for obtaining step a carries out centrifugation desugar, and it is rotten to obtain desugar Chinese yam；
Step c, purified water is added in desugar Chinese yam gruel, is warming up to 45-50 DEG C, adds pronase and digested, enzyme
Solution terminates to carry out enzyme deactivation, obtains the first enzymolysis liquid, wherein enzymatic hydrolysis condition are as follows: enzymatic hydrolysis pH value is 7.8-8, enzymolysis time 2-3h；
Step d, the first enzymolysis liquid is cooled to 50-55 DEG C, neutral proteinase is added and flavor protease is digested, enzymatic hydrolysis knot
Shu Jinhang enzyme deactivation obtains the second enzymolysis liquid, wherein enzymatic hydrolysis condition are as follows: enzymatic hydrolysis pH value is 6-7, enzymolysis time 3-4h；
Step e, the second enzymolysis liquid obtains Chinese yam peptide after separation, purifying and drying.
2. the extracting method of Chinese yam peptide as described in claim 1, it is characterised in that: in step c, the pronase adds
Enter the 0.5-1% that amount is the Chinese yam quality.
3. the extracting method of Chinese yam peptide as described in claim 1, it is characterised in that: in step d, the neutral proteinase is by withered
Careless fermentation of bacillus forms, and additional amount is the 1-1.5% of the Chinese yam quality, and the additional amount of the flavor protease is institute
State the 0.3-0.5% of Chinese yam quality.
4. the extracting method of Chinese yam peptide as described in claim 1, it is characterised in that: in step e, second enzymolysis liquid is used
Three-phase centrifuge centrifugation, obtained centrifugate obtain the Chinese yam peptide of different molecular weight after nanofiltration, concentration and drying.
5. the extracting method of Chinese yam peptide as claimed in claim 4, it is characterised in that: the centrifugate is successively by nanofiltration membrane
1 nanometer, 0.2 nanometer and 0.5 nanometer of collecting and filtering apparatus separation, takes the liquid between 1-0.2 nanometers and 0.2-0.5 nanometers, after
Reason respectively obtains the Chinese yam peptide of 500-1000 dalton and 180-500 dalton.
6. the extracting method of Chinese yam peptide as described in claim 1, it is characterised in that: in step a, the amylase is solution gemma
Bacillus fermentation forms, and additional amount is the 1-1.5% of the Chinese yam quality；And/or
In step a, the carbohydrase is glucoamylase, ferments to obtain by aspergillus oryzae, and additional amount is the Chinese yam quality
0.5-0.8%；And/or
In step a, the desugar enzymatic hydrolysis condition are as follows: hydrolysis temperature are as follows: 55-58 DEG C, enzymolysis time 2-3h.
7. the extracting method of Chinese yam peptide as described in claim 1, it is characterised in that: in step a, Chinese yam is crushed, institute is added
The purified water stirring for stating 5-6 times of Chinese yam quality, under the conditions of 90 DEG C -95 DEG C, boiling 20-25min；And/or
In step c, the additional amount of the purified water is 3-4 times of the Chinese yam quality.
8. the extracting method of Chinese yam peptide as described in claim 1, it is characterised in that: the condition of the enzyme deactivation are as follows: enzyme-removal temperature
It is 90-95 DEG C, the enzyme deactivation time is 10-15min；And/or
The drying mode is spray drying.
9. a kind of Chinese yam peptide, it is characterised in that: mentioned using the extracting method of Chinese yam peptide as described in any one of claims 1 to 8
It obtains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811599594.7A CN109371091A (en) | 2018-12-26 | 2018-12-26 | A kind of Chinese yam peptide and its extracting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811599594.7A CN109371091A (en) | 2018-12-26 | 2018-12-26 | A kind of Chinese yam peptide and its extracting method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109371091A true CN109371091A (en) | 2019-02-22 |
Family
ID=65372226
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811599594.7A Pending CN109371091A (en) | 2018-12-26 | 2018-12-26 | A kind of Chinese yam peptide and its extracting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109371091A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835635A (en) * | 2012-09-29 | 2012-12-26 | 赵红丽 | Dioscorea opposita peptide polysaccharide composite nutrient solution prepared from dioscorea opposita serving as raw material and by biological enzyme method and preparation method of composite nutrient solution |
| CN109007691A (en) * | 2018-07-13 | 2018-12-18 | 河南京华食品科技开发有限公司 | Multistage enzymatic hydrolysis and spray drying technology combine the method for preparing the pure powder of iron rod yam |
-
2018
- 2018-12-26 CN CN201811599594.7A patent/CN109371091A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835635A (en) * | 2012-09-29 | 2012-12-26 | 赵红丽 | Dioscorea opposita peptide polysaccharide composite nutrient solution prepared from dioscorea opposita serving as raw material and by biological enzyme method and preparation method of composite nutrient solution |
| CN109007691A (en) * | 2018-07-13 | 2018-12-18 | 河南京华食品科技开发有限公司 | Multistage enzymatic hydrolysis and spray drying technology combine the method for preparing the pure powder of iron rod yam |
Non-Patent Citations (3)
| Title |
|---|
| 刘瑞山等: "双酶法分解怀山药的工艺研究", 《安徽农业科学》 * |
| 姜锡瑞等: "《生物发酵产业技术》", 31 May 2016, 中国轻工业出版社 * |
| 朱玉端等: "双酶法酶解制备怀山药多肽的研究", 《食品工业》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108085356B (en) | Method for industrially producing high-purity walnut peptide by taking low-temperature squeezed walnut meal as raw material | |
| CN104664384B (en) | A kind of preparation method and application of black garlic fermentation liquid | |
| CN102226144B (en) | Black garlic vinegar and preparation method thereof | |
| CN102488073B (en) | Extraction method of sea cucumber polypeptide | |
| CN105273934A (en) | Fermented sweet prickly pear wine and production method thereof | |
| CN111387497A (en) | High-sialic acid instant cubilose product and sterilization and preparation method thereof | |
| CN106509140A (en) | Fermented type soybean whey beverage and preparation method thereof | |
| CN102228125B (en) | Preparation method of algal active peptide | |
| CN106359839A (en) | Extraction method of oyster peptides | |
| CN106047975A (en) | Mussel protein peptide extraction method | |
| CN103735633A (en) | Preparation method of instant gardenia powder | |
| CN106889295A (en) | A kind of preparation method of live body sea stichopus japonicus active peptide | |
| CN110746515A (en) | Lycium barbarum polysaccharide, lycium barbarum red element and lycium barbarum polypeptide prepared by synchronous separation and preparation method thereof | |
| CN105077169B (en) | One kind is rich in soybean polysaccharide health care soy sauce and preparation method thereof | |
| CN109730224A (en) | Oligopeptide rose enzyme new function drink | |
| CN109439717A (en) | A kind of extracting method of small molecule rhizoma polygonati polypeptide | |
| CN109457008B (en) | Preparation method of ginseng chelating peptide | |
| CN111938062A (en) | Oyster protein solid beverage rich in biological zinc and preparation method thereof | |
| CN102090672B (en) | Short necked clam nutrient solution and preparation method thereof | |
| CN114343143A (en) | Marine algae fermentation product and preparation method thereof | |
| CN1544646A (en) | Bitterless soybean polypeptide and its production method | |
| CN109055462A (en) | A kind of extraction and preparation process of walnut polypeptide | |
| CN109371091A (en) | A kind of Chinese yam peptide and its extracting method | |
| CN114209067B (en) | Deodorization bee pupa peptide and preparation method and application thereof | |
| CN109576331A (en) | A kind of extracting method of wheat germ peptide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190222 |
|
| RJ01 | Rejection of invention patent application after publication |