CN109369477B - Equipment and method for extracting amino acid from donkey hair - Google Patents
Equipment and method for extracting amino acid from donkey hair Download PDFInfo
- Publication number
- CN109369477B CN109369477B CN201811451934.1A CN201811451934A CN109369477B CN 109369477 B CN109369477 B CN 109369477B CN 201811451934 A CN201811451934 A CN 201811451934A CN 109369477 B CN109369477 B CN 109369477B
- Authority
- CN
- China
- Prior art keywords
- amino acid
- donkey
- hair
- donkey hair
- degreasing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C319/00—Preparation of thiols, sulfides, hydropolysulfides or polysulfides
- C07C319/26—Separation; Purification; Stabilisation; Use of additives
- C07C319/28—Separation; Purification
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/14—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from compounds containing already amino and carboxyl groups or derivatives thereof
- C07C227/18—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from compounds containing already amino and carboxyl groups or derivatives thereof by reactions involving amino or carboxyl groups, e.g. hydrolysis of esters or amides, by formation of halides, salts or esters
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C319/00—Preparation of thiols, sulfides, hydropolysulfides or polysulfides
- C07C319/22—Preparation of thiols, sulfides, hydropolysulfides or polysulfides of hydropolysulfides or polysulfides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D207/02—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D207/04—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
- C07D207/10—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D207/16—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses equipment and a method for extracting amino acid from donkey hair, and belongs to the field of chemical extraction. The method comprises the following steps: recovering crude donkey hair, pretreating crude donkey hair, drying donkey hair, defatting, hydrolyzing defatted donkey hair, preparing refined leucine and preparing amino acid oral liquid; the effective degreasing treatment equipment is provided in the degreasing treatment of the dry donkey hair, the degreasing treatment integration of the donkey hair can be realized by using the degreasing treatment equipment, manpower and material resources are saved, the degreasing time is saved, the degreasing of the donkey hair can be effectively realized by combining the degreasing method of the dry donkey hair, the degreasing efficiency is improved, the hydrolysis effect in the subsequent process is convenient to increase, the yield of the amino acid can be effectively improved, resources can be saved, the amino acid oral liquid required by a human body can be prepared, and the environmental pollution is avoided.
Description
Technical Field
The invention relates to the field of chemical extraction, in particular to equipment and a method for extracting amino acid from donkey hair.
Background
Amino acids are important components of biological organisms, are basic units constituting proteins, and have extremely important physiological functions. Over the last century, human beings have continuously revealed the secrecy of proteins, so that the production of amino acid products has been developed rapidly. In 2012, the yield of amino acid reaches 330 ten thousand tons, and the amino acid in China is greatly improved in the aspects of new product development and production and application at present. At present, in addition to applications in the fields of medicines, foods, feeds, cosmetics and the like, amino acid products and derivatives of the amino acid products are continuously applied in the fields of agricultural production, chemical industry and the like. The amino acid products currently on the market are various and mainly comprise isoleucine, valine, leucine, proline, threonine, glutamic acid, arginine, lysine acetate, tryptophan, tyrosine, cystine, asparagine, compound amino acid and the like. Cystine is a sulfur-containing amino acid, which is present in small amounts in proteins and is contained in keratin such as hair and finger claws. L-cystine is one of amino acids essential to human body, and has important value in medicine and as nutrition enhancer, and also in food industry. Cystine is amino acid medicine, and has effects of promoting cell oxidation reduction function, invigorating liver function, neutralizing toxin, promoting leukocyte proliferation, and preventing pathogenic bacteria development; vitamin B1 binds adenosine triphosphate to form vitamin B1 pyrophosphate, a coenzyme necessary for carbohydrate metabolism; vitamin B1 can inhibit cholinesterase activity, increase cholinesterase activity in deficiency, accelerate acetylcholine hydrolysis, cause nerve impulse conduction disorder, and affect gastrointestinal and myocardial functions; calcium pantothenate is a precursor of coenzyme A, is a substance essential for various metabolic links, can participate in the synthesis of steroids, porphyrin, acetylcholine and other substances, and can maintain normal epithelial functions. It is mainly used for treating alopecia, acute infectious diseases such as dysentery, typhoid fever, influenza, asthma, neuralgia, eczema and various poisoning diseases, and has protein configuration maintaining effect. Also useful as a food flavoring agent. At present, the production process of cystine mainly comprises an extraction method, a chemical synthesis method, an enzyme catalysis method, a microbial fermentation method and the like. The hair is rich in various amino acids, such as cystine, leucine, arginine and the like, is a cheap amino acid source, and considerable economic benefit can be obtained when the amino acid is produced from the hair. The yield of cystine industrially extracted from hair can reach 7.5-8%, and only 5% in actual production, the yield is low, in the process of extracting amino acid from hair, the hair degreasing is particularly concerned, incomplete hair degreasing greatly influences the hydrolysis efficiency of hair, so that the yield of amino acid in actual production is lower and the quality is poor, and therefore, an extraction method with high yield of amino acid and small discharge amount of waste water is urgently needed.
Disclosure of Invention
The invention aims to provide equipment and a method for extracting amino acid from donkey hair, which aim to solve the problem that the efficiency of hair hydrolysis is influenced by low hair degreasing efficiency in the process of extracting amino acid from hair, and can improve the efficiency of hair hydrolysis and the yield of amino acid.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for extracting amino acid from donkey hair comprises the following steps:
1) recovering crude donkey hair; intensively treating and collecting donkey skins peeled off after slaughtering meat donkeys in a slaughterhouse, shaving rough donkey hairs on the donkey skins by using a mechanical scraper, collecting the donkey skins to be used for preparing donkey-hide gelatin, loading the rough donkey hairs on a truck for storage, and transporting the rough donkey hairs to a region to be treated;
2) pre-treating crude donkey hair; removing the rough donkey hair, firstly washing with water, cleaning stains such as bloodstain on the surface of the rough donkey hair, then steaming at high temperature, further removing peculiar smell, stains and the like attached to the rough donkey hair, simultaneously sterilizing at high temperature, reducing pollution of bacteria and fungi, and finally placing the rough donkey hair after being steamed at high temperature on a flat and sufficiently-illuminated square in the area to be treated for airing to prepare dry donkey hair;
3) drying donkey hair and degreasing; placing the dried donkey hair into donkey hair degreasing equipment for rapid degreasing to prepare degreased donkey hair;
4) hydrolyzing the degreased donkey hair; controlling the placement amount of the degreased donkey hair and the addition amount of concentrated hydrochloric acid to be 1: 1.8-2, putting the degreased donkey hair and the concentrated hydrochloric acid into a hydrolysis cylinder, carrying out hydrolysis treatment for 9 hours, controlling the hydrolysis temperature to be 110-115 ℃, hydrolyzing to prepare mixed hydrolysate, then adding alkali liquor into the mixed hydrolysate, heating for neutralization, and standing for 20 hours to prepare a mixed solution of crude cystine I and compound amino acid liquor I;
5) preparing refined cystine; firstly, filtering and separating the mixed solution to obtain the rough cystine I and the compound amino acid solution I, then mixing and dissolving the rough cystine I and hydrochloric acid with the concentration of 6%, adding activated carbon for primary decoloration and purification, and preparing and obtaining rough cystine II and the compound amino acid solution II; mixing and dissolving the rough cystine II and ammonia water with the concentration of 6%, neutralizing and recrystallizing, then adding the hydrochloric acid with the concentration of 6% and the active carbon for secondary decolorization and purification, and preparing refined cystine and waste composite amino acid liquid III; the compound amino acid liquid I, the compound amino acid liquid II and the waste compound amino acid liquid III are used for refining and extracting various amino acids to prepare the amino acid oral liquid.
Preferably, the step of refining and extracting various amino acids to prepare the amino acid oral liquid in the step 5) comprises the following steps:
s1, preparation of various amino acids: mixing, concentrating and crystallizing the composite amino acid solution I, the composite amino acid solution II and the composite amino acid solution III, controlling the temperature, and regulating the pH value to prepare crude alanine and a composite amino acid solution IV, wherein the crude alanine is subjected to multiple decolorization and purification treatments to prepare refined alanine; concentrating and crystallizing the compound amino acid solution IV, controlling the temperature, and regulating the pH value to prepare crude glycine and a compound amino acid solution V, wherein the crude glycine is subjected to multiple decolorization and purification treatments to prepare refined glycine; concentrating and crystallizing the compound amino acid solution V, controlling the temperature, and regulating the pH value to prepare crude L-proline and a compound amino acid solution VI, wherein the crude L-proline is subjected to multiple decolorization and purification treatments to prepare refined L-proline; concentrating and crystallizing the composite amino acid solution VI, controlling the temperature, and regulating the pH value to prepare crude L-hydroxyproline and a composite amino acid solution VII, wherein the crude L-hydroxyproline is subjected to multiple decolorization and purification treatments to prepare refined L-hydroxyproline; the compound amino acid liquid VII is subjected to evaporative crystallization to prepare compound amino acid;
s2, mixing the refined alanine, the refined glycine, the refined L-proline and the refined L-hydroxyproline with edible water to prepare amino acid mother liquor, adding honey, compound vitamin and water into the amino acid mother liquor, uniformly mixing, filling, adopting a high-temperature instantaneous sterilization method, keeping the temperature at 90 ℃, controlling the time for 10 minutes, sampling, checking to be qualified, and packaging to prepare the amino acid oral liquid;
provides a compound amino acid solution after the preparation of cystine, which makes full use of the amino acid solution and saves resources. Avoiding waste.
Preferably, the alkali liquor in the step 4) adopts ammonia water, and the temperature for heating and neutralizing is set to be 40 ℃; effectively promote hydrolysis neutralization.
Preferably, the step 4) further comprises a hydrolysis degree test, a small amount of mixed hydrolysate is taken, a 15% sodium hydroxide solution is added, a few drops of a copper sulfate solution are dripped into the mixed hydrolysate by slight heating, if the solution keeps blue, the hydrolysis is complete, and if the solution does not show blue, the hydrolysis is continued until the hydrolysis is complete; the connection hydrolysis degree is convenient.
An equipment for extracting amino acid from donkey hair, which is applied to a method for extracting amino acid from donkey hair:
the donkey-hide gelatin donkey is fixedly installed in the lower end of the right side of the equipment box, a discharging and conveying belt is fixedly installed at the discharging port, a box door is;
the donkey hair degreasing device comprises a degreasing cylinder body, the top of the degreasing cylinder body is respectively communicated with an acid liquor inlet, an alkali liquor inlet and a distilled water inlet, a circular ring feeding slideway is arranged at the top of the degreasing cylinder body, an electric control rotary cutter is fixedly arranged on the inner wall of the top of the degreasing cylinder body, wall sliding grooves are arranged on the upper portions of the inner walls of the left side and the right side of the degreasing cylinder body, the wall sliding grooves are in sliding connection with an electric control sliding block, the outer end of the electric control sliding block is fixedly connected with the outer surface of a circular ring supporting frame, the circular ring supporting frame is provided with a circular ring sliding rail, the circular ring sliding rail is in sliding connection with a track connecting block, the track connecting block is fixedly connected with the outer surface of the upper end of a containing cylinder, the outer surface of the containing cylinder is provided with a filter hole, the improved amino acid liquid storage tank is characterized in that a side cavity is formed in the periphery of the inner groove, a composite amino acid liquid pipe is fixedly connected to the bottom of the side cavity and communicated with a composite amino acid liquid tank, a throttling valve is arranged on the composite amino acid liquid pipe, torsion hinges are arranged on the left side and the right side of an opening at the upper end of the inner groove, and the torsion hinges are hinged to the sealing baffle.
Compared with the prior art, the invention provides equipment and a method for extracting amino acid from donkey hair, and the equipment and the method have the following beneficial effects:
(1) the invention provides a method for extracting amino acid by using donkey hair, which is characterized in that donkey hair fallen off after slaughtering donkey in a slaughterhouse is recycled, collected uniformly and transported to a region to be treated, the rough donkey hair is pretreated in the region to be treated, stains such as blood stain and the like on the surface of the rough donkey hair are removed, the surface of the rough donkey hair is clean, and then the rough donkey hair is steamed and softened at high temperature; the donkey hair is quickly degreased in a specially-made donkey hair degreasing device, the fat coverage on the surface of donkey hair is effectively reduced, the hydrolysis efficiency is conveniently increased in the process of chemically extracting amino acid, the yield of the amino acid is further improved, the refined cystine can be effectively produced in the chemical extraction process, meanwhile, the composite amino acid liquid produced in the production process is used for waste liquid treatment, the environmental pollution is avoided, the resources are effectively saved, the production cost is reduced, and the mixed fertilizer of the amino acid and ammonium chloride can be prepared in the waste liquid treatment for agricultural production and use, and the pesticide can also be manufactured for pest killing;
(2) the invention provides donkey hair degreasing equipment, which is used by combining a donkey hair degreasing method, dry donkey hair is placed on a feeding conveyor belt and conveyed to a feeding hopper to be poured into an equipment box body, the dry donkey hair can slide into a containing cylinder along a circular feeding slideway and can be contacted with an electric control rotary cutter arranged at the top end of a degreasing cylinder body in the sliding process, the dry donkey hair is more exquisite under the quick rotary cutting of the electric control rotary cutter, the hydrolysis area is increased, the hydrolysis efficiency is improved, after certain dry donkey hair is contained, an electric control slide block is controlled by a control panel to slide and move downwards in a wall sliding chute, the containing cylinder is moved downwards to the lower end of the degreasing cylinder body, then a proper amount of dilute hydrochloric acid is filled into the degreasing cylinder body through an acid liquid inlet, the dilute hydrochloric acid is intermittently stirred and soaked for a plurality of hours, a throttle valve is regulated and controlled, so that waste acid liquid is moved to a composite, then distilled water is poured into the tank through a distilled water inlet for cleaning for a plurality of times, the wastewater liquid is moved to a compound amino acid liquid tank, then pouring a proper amount of sodium hydroxide solution into the alkali liquor inlet, intermittently stirring and soaking for a plurality of hours, washing for a plurality of times by distilled water until the pH value of the washing solution is neutral, after acid-base degreasing is finished, the track connecting block is controlled to move quickly on a circular slide rail in the circular support frame, the rotation of the containing cylinder on the circular support frame is realized, huge centrifugal force is generated for drying, then the electric control slide block is continuously controlled to move downwards, so that the bottom of the containing cylinder extrudes the sealing baffle of the inner groove, the bottom of the containing cylinder is positioned above the discharging conveyor belt, the electric control drive rotating shaft is controlled to rotate so that the bottom cover plate is opened, the degreased donkey hair is conveyed out through the discharging conveyor belt, and a drying device can be arranged on the discharging conveyor belt to facilitate the drying of the wet degreased donkey hair; the donkey hair can be effectively degreased by combining donkey hair degreasing equipment and a degreasing method, so that the degreasing efficiency is improved, the hydrolysis effect in the subsequent process is convenient to increase, and the yield of the cystine is effectively improved;
(3) the invention provides a method for preparing amino acid oral liquid, wherein the solubilities of different amino acids have certain difference, through the difference, the composite amino acid liquid I, the composite amino acid liquid II and the composite amino acid liquid III left after the cystine preparation in the step 5) can be recycled and mixed through a certain recycling mechanism, then the temperature and the PH value are adjusted in real time, the composite amino acid liquid I, the composite amino acid liquid II and the composite amino acid liquid III are concentrated and crystallized through a concentration and crystallization device, the amino acids of different types are prepared after separation and purification, in the preparation process, the composite amino acid liquid I, the composite amino acid liquid II and the composite amino acid liquid III are fully utilized, the composite amino acid waste liquid produced after the cystine preparation is concentrated, crystallized and filtered layer by layer, the composite amino acid waste liquid produced after the cystine preparation is fully utilized, the resources are effectively saved, the cost is reduced, the preparation of the, The preparation method comprises the steps of preparing amino acid oral liquid by using amino acid, mixing refined alanine, refined glycine, refined L-proline and refined L-hydroxyproline with edible water to prepare amino acid mother liquor, taking the amino acid mother liquor, adding honey, multivitamins and water, uniformly mixing and filling.
Drawings
FIG. 1 is a schematic structural diagram of the operation steps of an apparatus for extracting amino acid from donkey hair and a method thereof according to the present invention;
FIG. 2 is a schematic structural diagram of a donkey hair defatting apparatus for extracting amino acids from donkey hair and a method thereof according to the present invention;
FIG. 3 is a schematic structural diagram of the donkey hair degreasing equipment of the donkey hair amino acid extraction equipment and the method thereof according to the present invention, which is expanded in three-dimensional;
FIG. 4 is a schematic structural diagram of a front cross-sectional view of a donkey hair defatting device of the donkey hair amino acid extracting equipment and method thereof provided by the invention;
FIG. 5 is a schematic diagram of a ring support frame of the donkey hair amino acid extraction device and method according to the present invention, which is enlarged in three dimensions;
FIG. 6 is a schematic structural diagram of the amino acid oral liquid preparation steps of the donkey hair amino acid extraction equipment and the method thereof provided by the invention.
Description of the figure numbers: 1. an equipment box body; 2. a discharge port; 3. a box door; 4. a control panel; 5. a feed hopper; 6. a feed conveyor; 7, discharging and conveying belt; 8, an acid liquor inlet; 9. an alkali liquor inlet; 10. a distilled water inlet; 11. a donkey hair degreasing device; 12. a compound amino acid liquid tank; 13. a degreasing cylinder body; 14. a circular ring feeding slideway; 15. an electrically controlled rotary cutter; 16. a wall chute; 17. an electric control slide block; 18. a circular ring support frame; 19. a containing cylinder; 20. a filtration pore; 21. an electrically controlled drive shaft; 22. a bottom cover plate; 23. an inner groove; 24. a lateral cavity; 25. a compound amino acid liquid tube; 26. a throttle valve; 27. a torsion hinge; 28. sealing the baffle; 29. a circular ring slide rail; 30. track connecting block.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example 1: referring to fig. 1;
a method for extracting amino acid from donkey hair comprises the following steps:
1) recovering crude donkey hair; intensively treating and collecting donkey skins peeled off after slaughtering meat donkeys in a slaughterhouse, shaving rough donkey hairs on the donkey skins by using a mechanical scraper, collecting the donkey skins to be used for preparing donkey-hide gelatin, loading the rough donkey hairs on a truck for storage, and transporting the rough donkey hairs to a region to be treated;
2) pre-treating crude donkey hair; the method comprises the following steps of (1) removing the crude donkey hair, firstly washing with water, cleaning stains such as bloodstain on the surface of the crude donkey hair, then cooking at high temperature, further removing peculiar smell and stains attached to the crude donkey hair, simultaneously sterilizing at high temperature, reducing pollution of bacteria and fungi, and finally placing the crude donkey hair after being cooked at high temperature on a flat and sufficiently-illuminated square in a region to be treated for airing to prepare dry donkey hair;
3) drying donkey hair and degreasing; placing the dried donkey hair into donkey hair degreasing equipment for rapid degreasing to prepare degreased donkey hair;
4) hydrolyzing the degreased donkey hair; controlling the placement amount of the degreased donkey hair and the addition amount of concentrated hydrochloric acid to be 1: 1.8-2, putting the degreased donkey hair and the concentrated hydrochloric acid into a hydrolysis tank, carrying out hydrolysis treatment for 9 hours, controlling the hydrolysis temperature to be 110-115 ℃, hydrolyzing to prepare mixed hydrolysate, then adding alkali liquor into the mixed hydrolysate, heating for neutralization, and standing for 20 hours to prepare a mixed solution of crude cystine I and compound amino acid liquor I;
5) preparing refined cystine; firstly, filtering and separating the mixed solution to obtain crude cystine I and compound amino acid solution I, then mixing and dissolving the crude cystine I and hydrochloric acid with the concentration of 6%, adding activated carbon for primary decolorization and purification, and preparing crude cystine II and compound amino acid solution II; mixing and dissolving the rough cystine II and ammonia water with the concentration of 6%, neutralizing and recrystallizing, then adding hydrochloric acid with the concentration of 6% and active carbon for secondary decolorization and purification, and preparing refined cystine and waste composite amino acid liquid III; the compound amino acid liquid I, the compound amino acid liquid II and the waste compound amino acid liquid III are used for refining and extracting various amino acids to prepare the amino acid oral liquid.
And (3) further optimizing, wherein the alkali liquor in the step 4) adopts ammonia water, and the heating and neutralizing temperature is set to be 40 ℃.
The invention provides a method for extracting amino acid by using donkey hair, which is characterized in that donkey hair fallen off after slaughtering donkey in a slaughterhouse is recycled, collected uniformly and transported to a region to be treated, the rough donkey hair is pretreated in the region to be treated, stains such as blood stain and the like on the surface of the rough donkey hair are removed, the surface of the rough donkey hair is clean, and then the rough donkey hair is steamed and softened at high temperature; the donkey hair degreasing agent is quickly degreased in a special donkey hair degreasing device, fat coverage on the surface of donkey hair is effectively reduced, hydrolysis efficiency is conveniently increased in chemical extraction of amino acid, the yield of the amino acid is further improved, delicate cystine can be effectively produced in the chemical extraction process, meanwhile, the compound amino acid liquid produced in the production process is subjected to waste liquid treatment, environmental pollution is avoided, resources are effectively saved, production cost is reduced, and the compound amino acid liquid can be used for preparing amino acid and ammonium chloride mixed fertilizer in waste liquid treatment and used for agricultural production and can also be used for manufacturing insecticide and insecticide.
Example 2: referring to FIG. 6, the difference between the embodiments is that
5) The steps of refining and extracting various amino acids to prepare the amino acid oral liquid in the steps are as follows:
s1, preparation of various amino acids: mixing, concentrating and crystallizing the composite amino acid solution I, the composite amino acid solution II and the composite amino acid solution III, controlling the temperature, and regulating the pH value to prepare crude alanine and a composite amino acid solution IV, wherein the crude alanine is subjected to multiple decolorization and purification treatments to prepare refined alanine; concentrating and crystallizing the compound amino acid solution IV, controlling the temperature, regulating the pH value to prepare crude glycine and a compound amino acid solution V, and performing multiple decolorization and purification treatments on the crude glycine to prepare refined glycine; concentrating and crystallizing the compound amino acid solution V, controlling the temperature, and regulating the pH value to obtain crude L-proline and a compound amino acid solution VI, wherein the crude L-proline is subjected to multiple decolorization and purification treatments to prepare refined L-proline; concentrating and crystallizing the composite amino acid solution VI, controlling the temperature, and regulating the pH value to prepare crude L-hydroxyproline and a composite amino acid solution VII, wherein the crude L-hydroxyproline is subjected to multiple decolorization and purification treatments to prepare refined L-hydroxyproline; and (3) preparing the compound amino acid from the compound amino acid solution VII through evaporative crystallization.
S2, mixing refined alanine, refined glycine, refined L-proline and refined L-hydroxyproline with edible water to obtain amino acid mother liquor, adding honey, vitamin complex and water into the amino acid mother liquor, uniformly mixing, filling, adopting a high-temperature instantaneous sterilization method, keeping the temperature at 90 ℃, controlling the time for 10 minutes, sampling, checking, and packaging to obtain the amino acid oral liquid.
The solubility of different amino acids has certain difference, through the difference, the composite amino acid liquid I, the composite amino acid liquid II and the composite amino acid liquid III left after the preparation of the cystine in the step 5) can be recycled and mixed through a certain recycling mechanism, then the temperature and the PH value are adjusted in real time, the composite amino acid liquid I, the composite amino acid liquid II and the composite amino acid liquid III are concentrated and crystallized through a concentration and crystallization device, and different types of amino acids are prepared after separation and purification, in the preparation process, the composite amino acid liquid I, the composite amino acid liquid II and the composite amino acid liquid III are fully utilized, the composite amino acid waste liquid produced after the preparation of the cystine is fully utilized, the resources are effectively saved, the cost is reduced, the preparation of various amino acids in the step S1, the separation and the preparation of necessary amino acids of the donkey-hide gelatin such as alanine, the glycine, the L-proline, in the practical application, the variety of the compound vitamin can be changed, and the sucrose is used for replacing the honey to prepare different kinds of amino acid oral liquids, so that the diversity of products is realized.
Example 3: the difference is based on example 1;
4) the method also comprises the step of hydrolysis degree inspection, wherein a small amount of mixed hydrolysate is taken, a sodium hydroxide solution with the concentration of 15% is added, a few drops of copper sulfate solution are dripped into the mixed hydrolysate by slight heating, if the solution keeps blue, the hydrolysis is complete, and if the solution does not show blue, the hydrolysis is continued until the hydrolysis is complete.
And (3) testing the degree of hydrolysis: putting 1mL of mixed hydrolysate into a 10mL test tube, adding 4mL of 15% NAOH aqueous solution, slightly heating, and dripping 4-5 drops of CuSO4Shaking the solution evenly, if the solution keeps blue, indicating that the hydrolysis is complete; if the color is purple or red-orange, the hydrolysis is not complete; the hydrolysis effect can be effectively distinguished through the hydrolysis degree inspection, and the hydrolysis efficiency is favorably improved; the protein molecule contains peptide bonds, copper ions are coordinated with the peptide bonds under the strong alkaline environment to form a copper-containing cyclic mixture, the complex is purple or brown, and the peptide bonds are not broken after the protein is hydrolyzed, so the protein molecule does not have the color reaction even in an alkaline solution.
Example 4: referring to FIGS. 2-5; the difference is based on example 1;
the equipment for extracting the amino acid from the donkey hair is applied to a method for extracting the amino acid from the donkey hair:
the device comprises a device box body 1, wherein a discharge port 2 is arranged at the lower end of the right side of the device box body 1, a discharge conveying belt 7 is fixedly arranged at the discharge port 2, a box door 3 is arranged on the front surface of the device box body 1, a control panel 4 is fixedly arranged on the surface of the box door 3, a feeding conveying belt 6 is fixedly arranged at the left side of the device box body 1, a feeding hopper 5 is fixedly connected with the middle end of the top of the device box body 1, the feeding hopper 5 is matched with one end of the feeding conveying belt 6, an acid liquor inlet 8, an alkali liquor inlet 9 and a distilled water inlet 10 are respectively arranged at the top of the device;
the donkey hair degreasing device 11 comprises a degreasing cylinder body 13, the top of the degreasing cylinder body 13 is respectively communicated with an acid liquor inlet 8, an alkali liquor inlet 9 and a distilled water inlet 10, the top of the degreasing cylinder body 13 is provided with a circular ring feeding slideway 14, the inner wall of the top of the degreasing cylinder body 13 is fixedly provided with an electric control rotary cutter 15, the upper parts of the inner walls of the left side and the right side of the degreasing cylinder body 13 are provided with wall chutes 16, the wall chutes 16 are in sliding connection with electric control sliders 17, the outer ends of the electric control sliders 17 are fixedly connected with the outer surface of a circular ring supporting frame 18, the circular ring supporting frame 18 is provided with a circular ring sliding rail 29, the circular ring sliding rail 29 is in sliding connection with a rail connecting block 30, the rail connecting block 30 is fixedly connected with the outer surface of the upper end of a containing cylinder 19, the outer surface, the bottom of the degreasing cylinder body 13 is provided with an inner groove 23, the inner groove 23 is formed into a side cavity 24 around, the bottom of the side cavity 24 is fixedly connected with a composite amino acid liquid pipe 25, the composite amino acid liquid pipe 25 is communicated with the composite amino acid liquid box 12, a throttle valve 26 is arranged on the composite amino acid liquid pipe 25, the left side and the right side of an opening at the upper end of the inner groove 25 are provided with torsion hinges 27, and the torsion hinges 27 are hinged to a sealing baffle 28.
The dried donkey hair is placed on a feeding conveyor belt 6, conveyed and transported to a feeding hopper 5 and poured into an equipment box 1, the dried donkey hair can slide into a containing cylinder 19 along a circular feeding slideway 14 and can contact with an electric control rotary cutter 15 arranged at the top end of a degreasing cylinder body 13 in the sliding process, the dried donkey hair is more exquisite under the rapid rotation and cutting of the electric control rotary cutter 15, the hydrolysis area is increased, the hydrolysis efficiency is improved, after a certain amount of dried donkey hair is contained, an electric control slide block 17 is controlled by a control panel 4 to slide down on a wall sliding chute 16, the containing cylinder is moved down to the lower end of the degreasing cylinder body 13, then a proper amount of 3 percent dilute hydrochloric acid is filled into the degreasing cylinder body 13 through an acid liquid inlet 8, the mixture is stirred and soaked for a plurality of hours intermittently, a throttle valve 26 is regulated and controlled, the waste acid liquid is moved to a composite amino acid liquid tank 12 through a composite amino acid liquid pipe 25 of a side cavity 24, the waste liquid is moved to a compound amino acid liquid box 12, then a proper amount of 3 percent sodium hydroxide solution is filled through an alkali liquor inlet 9 to be intermittently stirred and soaked for a plurality of hours, then washing for several times by distilled water until the pH value of the washing liquid is neutral, after the acid-base degreasing is finished, the control track connecting block 30 moves fast on the circular slide rail 29 in the circular support frame 18, realizes the rotation of the containing cylinder 19 on the circular support frame 18, generates huge centrifugal force for drying, then, the electric control slide block 17 is continuously controlled to move downwards, the bottom of the containing cylinder 19 is extruded by the sealing baffle 28 of the inner groove, the bottom of the containing cylinder 19 is positioned above the discharging conveyor belt 7, the electric control driving rotating shaft 21 is controlled to rotate, the bottom cover plate 22 is opened, the degreased donkey hair is conveyed out through the discharging conveyor belt 7, and a drying device can be arranged on the discharging conveyor belt 7, so that the wet degreased donkey hair can be dried conveniently; the donkey hair defatting equipment and the defatting method are combined, so that the donkey hair defatting can be effectively realized, the defatting efficiency is improved, the hydrolysis effect in the subsequent process is conveniently increased, and the yield of the cystine is effectively improved.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (4)
1. An equipment for extracting amino acid from donkey hair is characterized by comprising an equipment box body (1), a discharge hole (2) is formed in the lower end of the right side of the equipment box body (1), a discharge conveying belt (7) is fixedly installed on the discharge hole (2), the front surface of the equipment box body (1) is provided with a box door (3), the surface of the box door (3) is fixedly provided with a control panel (4), a feeding conveyor belt (6) is fixedly arranged at the left side of the equipment box body (1), the middle end of the top of the equipment box body (1) is fixedly connected with a feed hopper (5), the feed hopper (5) is matched with one end of the feed conveyor belt (6), the top of the equipment box body (1) is also provided with an acid liquor inlet (8), an alkali liquor inlet (9) and a distilled water inlet (10) respectively, the equipment box body (1) is internally and fixedly provided with a donkey hair degreasing device (11) and a compound amino acid liquid box (12);
the donkey hair degreasing device (11) is including degreasing cylinder body (13), degreasing cylinder body (13) top communicates with acidizing fluid import (8), alkali lye import (9) and distilled water import (10) respectively, degreasing cylinder body (13) top is provided with ring feeding slide (14), degreasing cylinder body (13) top inner wall fixed mounting has automatically controlled rotary cutter (15), degreasing cylinder body (13) left and right sides inner wall upper portion is provided with wall spout (16), wall spout (16) and automatically controlled slider (17) sliding connection, automatically controlled slider (17) outer end and ring support frame (18) surface fixed connection, ring support frame (18) are provided with ring slide rail (29), ring slide rail (29) and track connection piece (30) sliding connection, track connection piece (30) and hold jar (19) upper end surface fixed connection, hold jar (19) surface and be provided with filtration pore (20), it is provided with automatically controlled drive pivot (21) to hold jar (19) bottom opening part left and right sides, automatically controlled drive pivot (21) and bottom apron (22) one side fixed connection, degrease cylinder body (13) bottom is provided with inner groovy (23), side chamber (24) are set to all around in inner groovy (23), side chamber (24) bottom fixedly connected with compound amino acid liquid pipe (25), compound amino acid liquid pipe (25) and compound amino acid liquid case (12) intercommunication, be provided with choke valve (26) on compound amino acid liquid pipe (25), the inner groovy (23) upper end opening part left and right sides is provided with torsion hinge (27), torsion hinge (27) are articulated with seal baffle (28).
2. A method for extracting amino acids from donkey hair by using the donkey hair amino acid extraction equipment of claim 1, which is characterized by comprising the following steps:
1) recovering crude donkey hair; intensively treating and collecting donkey skins peeled off after slaughtering meat donkeys in a slaughterhouse, shaving rough donkey hairs on the donkey skins by using a mechanical scraper, collecting the donkey skins to be used for preparing donkey-hide gelatin, loading the rough donkey hairs on a truck for storage, and transporting the rough donkey hairs to a region to be treated;
2) pre-treating crude donkey hair; removing the rough donkey hair, firstly washing with water, cleaning stains such as bloodstain on the surface of the rough donkey hair, then steaming at high temperature, further removing peculiar smell, stains and the like attached to the rough donkey hair, simultaneously sterilizing at high temperature, reducing pollution of bacteria and fungi, and finally placing the rough donkey hair after being steamed at high temperature on a flat and sufficiently-illuminated square in the area to be treated for airing to prepare dry donkey hair;
3) drying donkey hair and degreasing; placing the dried donkey hair into donkey hair degreasing equipment for rapid degreasing to prepare degreased donkey hair;
4) hydrolyzing the degreased donkey hair; controlling the placement amount of the degreased donkey hair and the addition amount of concentrated hydrochloric acid to be 1: 1.8-2, putting the degreased donkey hair and the concentrated hydrochloric acid into a hydrolysis cylinder, carrying out hydrolysis treatment for 9 hours, controlling the hydrolysis temperature to be 110-115 ℃, hydrolyzing to prepare mixed hydrolysate, then adding alkali liquor into the mixed hydrolysate, heating for neutralization, and standing for 20 hours to prepare a mixed solution of crude cystine I and compound amino acid liquor I;
5) preparing refined cystine; firstly, filtering and separating the mixed solution to obtain the rough cystine I and the compound amino acid solution I, then mixing and dissolving the rough cystine I and hydrochloric acid with the concentration of 6%, adding activated carbon for primary decoloration and purification, and preparing and obtaining rough cystine II and the compound amino acid solution II; mixing and dissolving the rough cystine II and ammonia water with the concentration of 6%, neutralizing and recrystallizing, then adding the hydrochloric acid with the concentration of 6% and the active carbon for secondary decolorization and purification, and preparing refined cystine and waste composite amino acid liquid III; the compound amino acid liquid I, the compound amino acid liquid II and the waste compound amino acid liquid III are used for refining and extracting various amino acids to prepare amino acid oral liquid;
the steps of refining and extracting various amino acids to prepare the amino acid oral liquid are as follows:
s1, preparation of various amino acids: mixing, concentrating and crystallizing the composite amino acid solution I, the composite amino acid solution II and the composite amino acid solution III, controlling the temperature, and regulating the pH value to prepare crude alanine and a composite amino acid solution IV, wherein the crude alanine is subjected to multiple decolorization and purification treatments to prepare refined alanine; concentrating and crystallizing the compound amino acid solution IV, controlling the temperature, and regulating the pH value to prepare crude glycine and a compound amino acid solution V, wherein the crude glycine is subjected to multiple decolorization and purification treatments to prepare refined glycine; concentrating and crystallizing the compound amino acid solution V, controlling the temperature, and regulating the pH value to prepare crude L-proline and a compound amino acid solution VI, wherein the crude L-proline is subjected to multiple decolorization and purification treatments to prepare refined L-proline; concentrating and crystallizing the composite amino acid solution VI, controlling the temperature, and regulating the pH value to prepare crude L-hydroxyproline and a composite amino acid solution VII, wherein the crude L-hydroxyproline is subjected to multiple decolorization and purification treatments to prepare refined L-hydroxyproline; the compound amino acid liquid VII is subjected to evaporative crystallization to prepare compound amino acid;
s2, mixing the refined alanine, the refined glycine, the refined L-proline and the refined L-hydroxyproline with edible water to prepare amino acid mother liquor, adding honey, compound vitamin and water into the amino acid mother liquor, uniformly mixing, filling, adopting a high-temperature instantaneous sterilization method, keeping the temperature at 90 ℃, controlling the time for 10 minutes, sampling, inspecting, packaging to obtain the amino acid oral liquid.
3. The method for extracting amino acids from donkey hair according to claim 2, wherein the method comprises the following steps: and 4) adopting ammonia water as the alkali liquor in the step 4), and setting the heating and neutralizing temperature to be 40 ℃.
4. The method for extracting amino acids from donkey hair according to claim 2, wherein the method comprises the following steps: and 4) also comprising a hydrolysis degree test, namely taking a small amount of mixed hydrolysate, adding a 15% sodium hydroxide solution, slightly heating, and dripping a few drops of a copper sulfate solution, wherein if the solution keeps blue, the hydrolysis is complete, and if the solution does not show blue, the hydrolysis is continued until the hydrolysis is complete.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811451934.1A CN109369477B (en) | 2018-11-30 | 2018-11-30 | Equipment and method for extracting amino acid from donkey hair |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811451934.1A CN109369477B (en) | 2018-11-30 | 2018-11-30 | Equipment and method for extracting amino acid from donkey hair |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109369477A CN109369477A (en) | 2019-02-22 |
| CN109369477B true CN109369477B (en) | 2020-09-11 |
Family
ID=65376384
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811451934.1A Active CN109369477B (en) | 2018-11-30 | 2018-11-30 | Equipment and method for extracting amino acid from donkey hair |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109369477B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113713422B (en) * | 2021-08-31 | 2022-04-29 | 连云港华昌生物工程有限公司 | Hair cystine extraction equipment and process with impurity removal function |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1167110A (en) * | 1997-04-11 | 1997-12-10 | 孙复兴 | Production of L-cystine using ox and donkey hair as raw material |
| CN1167109A (en) * | 1997-04-30 | 1997-12-10 | 青岛海洋大学 | Method for extracting cystine and composite amino-acid from yak hair |
| CN1247059A (en) * | 1999-08-18 | 2000-03-15 | 珲春图们江精细化工研究院 | Non-saccharide nutritive amino acid liquid |
| CN103787939A (en) * | 2014-03-12 | 2014-05-14 | 广州博采生物科技有限公司 | Method and device of separating and extracting amino acid products from protein hydrolysates |
-
2018
- 2018-11-30 CN CN201811451934.1A patent/CN109369477B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1167110A (en) * | 1997-04-11 | 1997-12-10 | 孙复兴 | Production of L-cystine using ox and donkey hair as raw material |
| CN1167109A (en) * | 1997-04-30 | 1997-12-10 | 青岛海洋大学 | Method for extracting cystine and composite amino-acid from yak hair |
| CN1247059A (en) * | 1999-08-18 | 2000-03-15 | 珲春图们江精细化工研究院 | Non-saccharide nutritive amino acid liquid |
| CN103787939A (en) * | 2014-03-12 | 2014-05-14 | 广州博采生物科技有限公司 | Method and device of separating and extracting amino acid products from protein hydrolysates |
Non-Patent Citations (1)
| Title |
|---|
| 废驴毛制取胱氨酸;李燕娟 等;《河北化工》;19911231(第3期);第15-16页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109369477A (en) | 2019-02-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104140474B (en) | A kind of method of utility in comprehensive utilizing shrimp Eriocheir sinensis skin | |
| CN100521959C (en) | Short peptide protein powder preparation method using livestock blood as material | |
| CN110684816A (en) | Preparation method and application of high-quality oyster protein peptide | |
| CN109369477B (en) | Equipment and method for extracting amino acid from donkey hair | |
| CN102351944B (en) | Method for extracting alfalfa leaf protein by ultrasonic-assisted enzyme process | |
| CN102150800A (en) | Pickled vegetable instant food producing process | |
| CN107278208A (en) | Chitin, hydrolysate and the mode by means of enzymatic hydrolysis are used for the method that at least one desired product of target is produced from insect, and it is included in the step of being performed before enzymatic hydrolysis and combined | |
| CN110684675A (en) | Blakeslea trispora fermentation method and product thereof | |
| CN102940246B (en) | Prawn biological protein calcium preparation method | |
| CN103952457A (en) | Method for extracting active collagen peptide with low molecular weight from chicken skin | |
| CN101548709A (en) | Animal blood peptone and processing process thereof | |
| CN110339113A (en) | A kind of peach colloidal solution and its preparation method and application | |
| US20110282042A1 (en) | Obtainment of chitin from shrimp waste by means of microwave and/or autoclaving in combination with organic acids in a single stage | |
| CN208653162U (en) | A kind of processing of aquatic products drying unit | |
| CN106165765A (en) | A kind of facilitating digestion absorbs high protein crab feed additive | |
| CN106306480B (en) | Additive comprising sodium glutamate | |
| CN113713422B (en) | Hair cystine extraction equipment and process with impurity removal function | |
| CN1656902A (en) | Fish noodle and its production method | |
| CN104222969A (en) | Sea cucumber juice seasoning and preparation method thereof | |
| KR100790007B1 (en) | A method for purification glycosaminoglycans from ascidian tunic | |
| CN204994555U (en) | Egg processor is made again to domestic type | |
| RU2333676C1 (en) | Method of edible mushroom nutritional value improvement | |
| CN216869041U (en) | Drying device for production and processing for preparation of acid hydrolysis plant protein liquid | |
| CN214746930U (en) | A even formula drying equipment for amino acid production | |
| CN220181938U (en) | Automatic leather feeding mechanism |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |