CN109364042B - Traditional Chinese medicine targeted tilmicosin micro-pill and preparation method thereof - Google Patents
Traditional Chinese medicine targeted tilmicosin micro-pill and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine targeted tilmicosin pellet and a preparation method thereof, wherein the traditional Chinese medicine targeted tilmicosin pellet comprises the following raw materials in parts by weight: 20 parts of tilmicosin; 5-10 parts of platycodon grandiflorum superfine powder; 1-8 parts of pubescent holly root superfine powder; disintegrating agent: 10-35 parts; adhesive: 5-10 parts; filling agent: 1-15 parts. The preparation method comprises the following steps: 1. respectively crushing platycodon grandiflorum and pubescent holly root, and sieving with a 350-mesh sieve to obtain superfine platycodon grandiflorum powder and superfine pubescent holly root powder; 2. dissolving rhizoma bletilla polysaccharide gum with water, and stirring to obtain rhizoma bletilla polysaccharide gum water solution; 3. stirring tilmicosin, radix Platycodi ultra-fine powder, radix Ilicis Pubescentis ultra-fine powder, disintegrating agent and filler for 10min, adding rhizoma Bletillae polysaccharide gum water solution, and stirring for 30min to obtain soft material; 4. the prepared soft material is granulated, shot-blasted, dried and coated to obtain the pellet. The invention can improve the effective concentration of tilmicosin in the lung and increase the absorption metabolic rate and the utilization rate of the medicament.
Description
Technical Field
The invention belongs to the field of traditional Chinese veterinary medicines, and particularly relates to a traditional Chinese medicine targeted tilmicosin pellet and a preparation method thereof.
Background
Tilmicosin is a special antibiotic for livestock and poultry semi-synthesized by a hydrolysate of tylosin, and has an inhibiting effect on gram-positive bacteria, some gram-negative bacteria, mycoplasma, spirochetes and the like; has stronger antibacterial activity to actinobacillus pleuropneumoniae and pasteurella than tylosin, and is mainly used for preventing and treating livestock pneumonia (caused by infection of actinobacillus pleuropneumoniae, pasteurella, mycoplasma and the like), avian mycoplasmosis and mastitis of lactating animals. Tilmicosin belongs to macrolide antibiotics, and particularly has a 16-membered ring lactone structure, can be combined with a 50S subunit of a ribosome of bacteria, acts on a P site, inhibits the combination of transferase, thereby hindering the shift of mRNA, and preventing peptide bond from being shifted from an A site to a P site, thereby inhibiting the synthesis of protein and generating a bacteriostatic effect.
At present, tilmicosin has two administration routes, namely oral administration and subcutaneous injection, and the subcutaneous administration mode has complicated clinical application, large toxic and side effect and relatively less use; the main problems of the oral administration mode are that: 1) tilmicosin is bitter in taste and is easy to reduce the appetite of livestock and poultry, so that the feed intake of the livestock and poultry is greatly reduced, and 2) the effective medicine concentration reaching the lung lesion is low and unstable, so that the curative effect of the tilmicosin is influenced.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a traditional Chinese medicine targeted tilmicosin pellet and a preparation method thereof, which not only can cover the bitter taste of tilmicosin, but also can improve the effective concentration of the tilmicosin in the lung and increase the absorption metabolic rate and the utilization rate of the medicine.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the first technical scheme is as follows:
a traditional Chinese medicine targeted tilmicosin pellet comprises the following raw materials in parts by weight:
further, the disintegrating agent is one or two of microcrystalline cellulose and sodium carboxymethyl cellulose.
Further, the disintegrant is microcrystalline cellulose.
Further, the adhesive is one or more of bletilla striata polysaccharide gum, dextrin and powdered sugar.
Further, the adhesive is bletilla striata polysaccharide gum.
Further, the filling agent is selected from one or two of astragalus polysaccharide and starch.
Further, the filler is selected from astragalus polysaccharide.
The second technical scheme is as follows:
a preparation method of a traditional Chinese medicine targeted tilmicosin pellet specifically comprises the following steps:
step 1, respectively crushing platycodon grandiflorum and pubescent holly root by using a jet mill, and sieving with a 350-mesh sieve to obtain superfine platycodon grandiflorum powder and superfine pubescent holly root powder for later use;
step 2, weighing the raw materials in parts by weight, and then mixing the raw materials in a mass ratio of the bletilla striata polysaccharide gum to the water of 2: 1, dissolving bletilla striata polysaccharide gum in water, and uniformly stirring to obtain a bletilla striata polysaccharide gum aqueous solution;
step 3, stirring tilmicosin, platycodon grandiflorum superfine powder, pubescent holly root superfine powder, microcrystalline cellulose and astragalus polysaccharide at the rotating speed of 60 revolutions per minute for 10 minutes, then adding the bletilla striata polysaccharide gum aqueous solution, and continuously stirring for 30 minutes to prepare a soft material;
and 4, granulating, shot blasting, drying and coating the prepared soft material to obtain the pellet.
Further, the step 4 specifically includes the following operations:
step 4-1, extruding and granulating the prepared soft material, and sieving with a 40-mesh sieve to obtain medicine granules;
step 4-2, performing shot blasting on the prepared medicine particles by using a shot blasting machine at the speed of 150 revolutions per minute until the pill cores are rounded, performing fluidized drying for 30 minutes, and naturally cooling to 45 ℃ to obtain the pill cores;
and 4-3, firstly dissolving the coating auxiliary materials by using a solvent to prepare a coating solution, then filling the cores into a multifunctional fluidized bed coating machine, and after the temperature of an air outlet is raised to 60 ℃, adding the cores and the coating auxiliary materials according to the mass ratio of the dry weight of the cores to the dry weight of the coating auxiliary materials of 8-15: 1, pumping the coating liquid into a spray gun through a peristaltic pump for spray coating (the frequency of the peristaltic pump is 70-80 times/min), and keeping the temperature of an air outlet at about 65 ℃ in the coating process; and after coating, continuously keeping the coating in a multifunctional fluidized bed coating machine for 15 minutes for drying.
Furthermore, the coating liquid comprises the following raw materials in parts by weight: 5-10 parts of coating auxiliary materials; solvent: 60 parts of absolute ethyl alcohol;
wherein the coating auxiliary material comprises 5-8 parts of hydroxypropyl methyl cellulose phthalate/polyacrylic resin; 1-3 parts of triethyl citrate; 1-2 parts of talcum powder.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention adopts the radix platycodi as a targeting carrier, and the tilmicosin is introduced into the lung channel, so that the distribution of the tilmicosin in lung tissues is improved, the effective concentration of the tilmicosin in the lung is improved, and the bioavailability of the tilmicosin is improved; in addition, the platycodon grandiflorum can also effectively increase the solubility of the tilmicosin, so that the absorption utilization rate of the tilmicosin is increased.
2. According to the invention, the pubescent holly root has the effects of promoting blood circulation to remove blood stasis, and a large number of experiments prove that the pubescent holly root can cooperate with the platycodon root to promote the function of guiding the platycodon root to the channels, so that the effect of the platycodon root in improving the effective concentration of tilmicosin in the lung is obviously enhanced; the single use of the radix ilicis does not have the effect, the radix ilicis is probably related to the effect of promoting blood circulation and removing blood stasis of the pubescent holly root, changes the blood flow change of lung tissues and the whole body by promoting blood circulation and removing blood stasis, is more favorable for leading the platycodon root to the channels and increasing the concentration of tilmicosin entering the lung; thereby achieving the purpose of improving the effective concentration of the tilmicosin in the lung tissue and indirectly increasing the absorption metabolic rate and the utilization rate of the medicament. In addition, the platycodon grandiflorum and the pubescent holly root can also play a role in masking the taste, and the problem of entrance of tilmicosin is solved.
3. The platycodon grandiflorum and the pubescent holly root adopt superfine powder, the medicine granularity is smaller, the particle diameter of 350 meshes is 25 mu m, the cell diameter of plants is 10-100 mu m, and 350 meshes are selected, so that the medicine can achieve the effect of breaking the wall, and the medicine effect of the medicine can be better exerted.
4. The invention takes microcrystalline cellulose as a disintegrating agent, bletilla striata polysaccharide gum as an adhesive, astragalus polysaccharide as a filling agent and hydroxypropyl methyl cellulose phthalate, triethyl citrate, talcum powder and solvent absolute ethyl alcohol for coating, can realize the controlled release of the medicament, enables the medicament to be released and dissolved in intestinal tracts, and avoids medicament irritation and degradation caused by the release of the medicament in the stomach.
The platycodon grandiflorum and the pubescent holly root can improve the effective concentration of tilmicosin in the lung, meanwhile, the platycodon grandiflorum has the effects of relieving cough, reducing phlegm, ventilating the lung and relieving asthma, the pubescent holly root also has the effects of clearing heat and removing toxicity, the medicines are combined, the medicines have synergistic effect, the effects supplement each other, and the functions of treating the infection diseases of the lung of the pig (such as swine plague, asthma, blue ears and the like) are jointly realized.
Detailed Description
The present invention will be described in further detail with reference to examples.
Example 1
A traditional Chinese medicine targeted tilmicosin pellet comprises the following raw materials by weight:
the preparation method comprises the following steps:
and step 1, respectively crushing the platycodon grandiflorum and the pubescent holly root by using a jet mill, and sieving by using a 350-mesh sieve to obtain superfine platycodon grandiflorum powder and superfine pubescent holly root powder for later use.
Step 2, weighing the raw materials according to the weight ratio of the bletilla striata polysaccharide gum to the water of 2: 1, dissolving bletilla striata polysaccharide gum in water, and uniformly stirring to obtain a bletilla striata polysaccharide gum aqueous solution;
step 3, stirring tilmicosin, platycodon grandiflorum superfine powder, pubescent holly root superfine powder, microcrystalline cellulose and astragalus polysaccharide for 10 minutes at the rotating speed of 60 revolutions per minute by adopting a groove type mixer, then adding the bletilla striata polysaccharide gum aqueous solution, and continuously stirring for 30 minutes to prepare a soft material;
step 4, granulating, shot blasting, drying and coating the prepared soft material to obtain pellets;
step 4-1, extruding and granulating the prepared soft material, and sieving with a 40-mesh sieve to obtain medicine granules;
step 4-2, performing shot blasting on the prepared medicine particles by using a shot blasting machine at the speed of 150 revolutions per minute until the pill cores are rounded, performing fluidized drying for 30 minutes, and naturally cooling to 45 ℃ to obtain the pill cores;
step 4-3, dissolving coating auxiliary materials of hydroxypropyl methyl cellulose phthalate, triethyl citrate and talcum powder by using solvent absolute ethyl alcohol to prepare a coating solution, then loading the pellet core into a multifunctional fluidized bed coating machine, and after the temperature of an air outlet is raised to 60 ℃, adding the coating solution into the multifunctional fluidized bed coating machine according to the mass ratio of the dry weight of the pellet core to the dry weight of the coating auxiliary materials of 10: 1, pumping the coating liquid into a spray gun for spray coating through a peristaltic pump (the frequency of the peristaltic pump is 75 times/min), and keeping the temperature of an air outlet at about 65 ℃ in the coating process; and after coating, continuously keeping the coating in a multifunctional fluidized bed coating machine for 15 minutes for drying.
Example 2
A traditional Chinese medicine targeted tilmicosin pellet comprises the following raw materials by weight:
the preparation method comprises the following steps: the same as in example 1.
Example 3
A traditional Chinese medicine targeted tilmicosin pellet comprises the following raw materials by weight:
the preparation method comprises the following steps: the same as in example 1.
Comparative example 1
A traditional Chinese medicine targeted tilmicosin pellet comprises the following raw materials by weight:
the preparation method comprises the following steps:
step 1, crushing platycodon grandiflorum by using an airflow crusher, and sieving the crushed platycodon grandiflorum by using a 350-mesh sieve to obtain superfine platycodon grandiflorum powder for later use;
step 2, weighing the raw materials according to the weight ratio of the bletilla striata polysaccharide gum to the water of 2: 1, dissolving bletilla striata polysaccharide gum in water, and uniformly stirring to obtain a bletilla striata polysaccharide gum aqueous solution;
step 3, stirring tilmicosin, platycodon grandiflorum superfine powder, microcrystalline cellulose and astragalus polysaccharide for 10 minutes at the rotating speed of 60 revolutions/min by adopting a groove type mixer, then adding the bletilla striata polysaccharide gum aqueous solution, and continuously stirring for 30 minutes to prepare a soft material;
step 4, granulating, shot blasting, drying and coating the prepared soft material to obtain pellets;
step 4-1, extruding and granulating the prepared soft material, and sieving with a 40-mesh sieve to obtain medicine granules;
step 4-2, performing shot blasting on the prepared medicine particles by using a shot blasting machine at the speed of 150 revolutions per minute until the pill cores are rounded, performing fluidized drying for 30 minutes, and naturally cooling to 45 ℃ to obtain the pill cores;
step 4-3, dissolving coating auxiliary materials of hydroxypropyl methyl cellulose phthalate, triethyl citrate and talcum powder by using solvent absolute ethyl alcohol to prepare a coating solution, then loading the pellet core into a multifunctional fluidized bed coating machine, and after the temperature of an air outlet is raised to 60 ℃, adding the coating solution into the multifunctional fluidized bed coating machine according to the mass ratio of the dry weight of the pellet core to the dry weight of the coating auxiliary materials of 10: 1, pumping the coating liquid into a spray gun for spray coating through a peristaltic pump (the frequency of the peristaltic pump is 75 times/min), and keeping the temperature of an air outlet at about 65 ℃ in the coating process; and after coating, continuously keeping the coating in a multifunctional fluidized bed coating machine for 15 minutes for drying.
Comparative example 2
A traditional Chinese medicine targeted tilmicosin pellet comprises the following raw materials by weight:
the preparation method comprises the following steps:
step 1, crushing the pubescent holly root by using a jet mill, and sieving by using a 350-mesh sieve to obtain pubescent holly root superfine powder for later use.
Step 2, weighing the raw materials in parts by weight, and then mixing the raw materials in a mass ratio of the bletilla striata polysaccharide gum to the water of 2: 1, dissolving bletilla striata polysaccharide gum in water, and uniformly stirring to obtain a bletilla striata polysaccharide gum aqueous solution;
step 3, stirring tilmicosin, the pubescent holly root superfine powder, the microcrystalline cellulose and the astragalus polysaccharide for 10 minutes at the rotating speed of 60 revolutions per minute by adopting a groove type mixer, then adding the bletilla polysaccharide gum aqueous solution, and continuously stirring for 30 minutes to prepare a soft material;
step 4, granulating, shot blasting, drying and coating the prepared soft material to obtain pellets;
step 4-1, extruding and granulating the prepared soft material, and sieving with a 40-mesh sieve to obtain medicine granules;
step 4-2, performing shot blasting on the prepared medicine particles by using a shot blasting machine at the speed of 150 revolutions per minute until the pill cores are rounded, performing fluidized drying for 30 minutes, and naturally cooling to 45 ℃ to obtain the pill cores;
step 4-3, dissolving coating auxiliary materials of hydroxypropyl methyl cellulose phthalate, triethyl citrate and talcum powder by using solvent absolute ethyl alcohol to prepare a coating solution, then loading the pellet core into a multifunctional fluidized bed coating machine, and after the temperature of an air outlet is raised to 60 ℃, adding the coating solution into the multifunctional fluidized bed coating machine according to the mass ratio of the dry weight of the pellet core to the dry weight of the coating auxiliary materials of 10: 1, pumping the coating liquid into a spray gun for spray coating through a peristaltic pump (the frequency of the peristaltic pump is 75 times/min), and keeping the temperature of an air outlet at about 65 ℃ in the coating process; and after coating, continuously keeping the coating in a multifunctional fluidized bed coating machine for 15 minutes for drying.
Comparative example 3
A traditional Chinese medicine targeted tilmicosin pellet comprises the following raw materials by weight:
the preparation method comprises the following steps:
step 1, crushing the white mulberry root-bark by using a pneumatic crusher, and sieving the crushed white mulberry root-bark by using a 350-mesh sieve to obtain white mulberry root-bark superfine powder for later use.
Step 2, weighing the raw materials according to the weight ratio of the bletilla striata polysaccharide gum to the water of 2: 1, dissolving bletilla striata polysaccharide gum in water, and uniformly stirring to obtain a bletilla striata polysaccharide gum aqueous solution;
step 3, stirring tilmicosin, the white mulberry root-bark superfine powder, the microcrystalline cellulose and the astragalus polysaccharide for 10 minutes at the rotating speed of 60 revolutions per minute by adopting a groove type mixer, then adding the bletilla polysaccharide gum aqueous solution, and continuously stirring for 30 minutes to prepare a soft material;
step 4, granulating, shot blasting, drying and coating the prepared soft material to obtain pellets;
step 4-1, extruding and granulating the prepared soft material, and sieving with a 40-mesh sieve to obtain medicine granules;
step 4-2, performing shot blasting on the prepared medicine particles by using a shot blasting machine at the speed of 150 revolutions per minute until the pill cores are rounded, performing fluidized drying for 30 minutes, and naturally cooling to 45 ℃ to obtain the pill cores;
step 4-3, dissolving coating auxiliary materials of hydroxypropyl methyl cellulose phthalate, triethyl citrate and talcum powder by using solvent absolute ethyl alcohol to prepare a coating solution, then loading the pellet core into a multifunctional fluidized bed coating machine, and after the temperature of an air outlet is raised to 60 ℃, adding the coating solution into the multifunctional fluidized bed coating machine according to the mass ratio of the dry weight of the pellet core to the dry weight of the coating auxiliary materials of 10: 1, pumping the coating liquid into a spray gun for spray coating through a peristaltic pump (the frequency of the peristaltic pump is 75 times/min), and keeping the temperature of an air outlet at about 65 ℃ in the coating process; and after coating, continuously keeping the coating in a multifunctional fluidized bed coating machine for 15 minutes for drying.
Comparative example 4
A traditional Chinese medicine targeted tilmicosin pellet comprises the following raw materials by weight:
the preparation method comprises the following steps:
step 1, weighing the raw materials according to the weight ratio of the bletilla striata polysaccharide gum to the water of 2: 1, dissolving bletilla striata polysaccharide gum in water, and uniformly stirring to obtain a bletilla striata polysaccharide gum aqueous solution;
step 2, stirring tilmicosin, microcrystalline cellulose and astragalus polysaccharide for 10 minutes at the rotating speed of 60 revolutions per minute by adopting a groove type mixer, then adding the bletilla striata polysaccharide gum aqueous solution, and continuously stirring for 30 minutes to prepare a soft material;
step 3, granulating, shot blasting, drying and coating the prepared soft material to obtain pellets;
step 3-1, extruding and granulating the prepared soft material, and sieving with a 40-mesh sieve to obtain medicine granules;
step 3-2, performing shot blasting on the prepared medicine particles by using a shot blasting machine at the speed of 150 revolutions per minute until the pill cores are rounded, performing fluidized drying for 30 minutes, and naturally cooling to 45 ℃ to obtain the pill cores;
3-3, dissolving coating auxiliary materials of hydroxypropyl methyl cellulose phthalate, triethyl citrate and talcum powder by using solvent absolute ethyl alcohol to prepare a coating solution, then loading the pellet core into a multifunctional fluidized bed coating machine, and after the temperature of an air outlet is raised to 60 ℃, adding the coating solution into the multifunctional fluidized bed coating machine according to the mass ratio of the dry weight of the pellet core to the dry weight of the coating auxiliary materials of 10: 1, pumping the coating liquid into a spray gun for spray coating through a peristaltic pump (the frequency of the peristaltic pump is 75 times/min), and keeping the temperature of an air outlet at about 65 ℃ in the coating process; and after coating, continuously keeping the coating in a multifunctional fluidized bed coating machine for 15 minutes for drying.
Effect example 1: time curve of lung
Firstly, sampling:
the healthy pigs are divided into 5 groups at random, wherein the groups are respectively a test group, a comparison two group, a comparison three group and a comparison four group, and tilmicosin is respectively expressed according to the weight per kilogram: feeding tilmicosin at a dosage of 40mg/kg (feed additive dosage is 400mg/kg) with a dosage (each test group and a control group are fed with the dosage converted according to the actual content of the tilmicosin), slaughtering at 0.25, 0.5, 1, 2, 4, 6, 8, 12, 24, 36, 48, 72 and 96h after feeding, aseptically taking lung tissues, washing the lung tissues with normal saline, cutting the lung tissues into small pieces, homogenizing by a high-speed homogenizer at 12000rpm, freezing and storing at the temperature of-20 ℃, and measuring the content of the tilmicosin, wherein the curve data of the drug administration is shown in table 1.
Two, grouping situation
One set of experiments was: the pellets prepared in example 1 were used for administration;
comparing one group: the pellets prepared in comparative example 1 were used for administration;
two groups are compared: the pellets prepared in comparative example 2 were used for administration;
three groups were compared: the pellets prepared in comparative example 3 were used for administration;
four groups were compared: the pellets prepared in comparative example 4 were used for administration;
thirdly, a tilmicosin determination method: adopting an HPLC method; the method comprises the following specific steps:
1. sample pretreatment:
weighing 5.0g of lung tissue, placing the lung tissue in a 50ml polypropylene centrifugal tube, adding 8ml of acetonitrile, whirling and uniformly mixing, shaking at a medium speed for 30min, centrifuging at 3000rpm for 10min, taking supernatant liquid to place in a 100ml centrifugal tube, respectively adding 5ml of potassium dihydrogen phosphate buffer solution and 8ml of acetonitrile into tissue clots, stirring to disperse the tissue clots, whirling and uniformly mixing, shaking at a medium speed for 30min, centrifuging at 3000rpm for 10min, combining the two supernatants in a 100ml centrifugal tube, adding 40ml of water, centrifuging at 3000rpml for 10min, taking supernatant liquid to place in a proper amount of container, adding 10ml of water, uniformly mixing, and passing through an SPE column.
Passing the extract through SPE column, activating with 10ml methanol and balancing SPE column with 10ml purification, adjusting vacuum pump pressure to make flow rate not too fast when sample passes SPE column, keeping flow rate stable, washing with 25% acetonitrile-pure water-pure acetonitrile washing method with flow rate of 1 drop/second, drying SPE column for at least 5min after acetonitrile flows out from SPE column, eluting with 2.5ml eluent in graduated tube, evaporating to dryness with rotary evaporator, diluting mobile phase to constant volume to 1ml, vortexing for 30s, standing for 15min, filtering with 0.22 micron pinhole type filter membrane, taking 20 microliter to HPLC analysis.
2. Chromatographic conditions are as follows:
a chromatographic column: c18 chromatographic column 150 x 4.6mm 5 μm
Mobile phase: acetonitrile: tetrahydrofuran: dibutylamine phosphate buffer: water 130:55:25:790
Flow rate: 0.7ml/min
Column temperature: 30 deg.C
TABLE 1
The results show that: compared with four groups, one group, two groups and three groups, the drug concentration of tilmicosin in lung tissues is obviously lower than that of one group, so that the ilex pubescens can be proved to be capable of cooperating with the platycodon grandiflorum to promote the meridian tropism of the platycodon grandiflorum as an introduction agent, and the effect of the platycodon grandiflorum on improving the effective concentration of the tilmicosin in the lung is obviously enhanced. In addition, the control group reached a peak in blood at 2 hours, which may be due to altered blood flow, but the overall effect was still inferior to that of the control and test groups.
Effect example 2: release Profile
The operational procedures described in appendix 160 in the veterinary pharmacopoeia of 2015 edition were used to determine the drug dissolution and release rates, and the pellets prepared in example 1 were subjected to disintegration tests, the release curve data of which are shown in table 2;
TABLE 2
As can be seen from the table, the prepared enteric-coated pellets can realize local release and reach the quality standard of enteric-coated preparations.
Effect example 3: clinical trial effect analysis
The clinical naturally-occurring pig herd, 116 fattening pigs, part of the body temperature is raised, cough and asthma occur, and foam-like liquid flows out of the mouth and the nose; dyspnea into a dog sitting posture; inflammatory swelling of the neck and throat; c, performing autopsy: the subcutaneous, thoracic and pericardial cavities are filled with fluid; bleeding and swelling of liver, lung, lymph nodes; the section of lung is marbled; the swine plague is diagnosed, the 104 surviving pigs are randomly divided into 4 groups, the groups are respectively treated by administration, the dosage is 400mg of tilmicosin per kilogram of feed additive, the whole group of the feed is administered by mixing, the treatment result is recorded, the treatment result is shown in the following table 3 and table 4, and during administration, each test group and each comparison group are administered by converting the actual content of the tilmicosin.
Grouping condition: see table below;
one set of experiments was: the pellets prepared in example 1 were used for administration;
comparing one group: the pellets prepared in comparative example 1 were used for administration;
two groups are compared: the pellets prepared in comparative example 4 were used for administration;
conventional drug groups: tilmicosin powder.
TABLE 3
| Grouping | Total number of heads | Number of clinical symptoms | Assume healthy head count |
| Test group | 26 heads | 15 heads | 11 heads |
| Compare a group | 26 heads | 13 heads | 13 heads |
| Comparison of two groups | 26 heads | 13 heads | 13 heads |
| Conventional drug group | 26 heads | 12 heads | 14 heads |
TABLE 4
| Grouping | Effective in prevention (head) | High efficiency | Number of heads cured | Cure rate | Time to cure |
| Test A set | 11 heads | 100％ | 15 heads | 100％ | 3 days |
| Compare a group | 13 heads | 100％ | 12 heads | 92.3％ | 4 days |
| Comparison of two groups | 12 heads | 92.3％ | 11 heads | 84.6％ | 5 days |
| Conventional drug group | 11 heads | 91.7％ | 11 heads | 78.6％ | 7 days |
The experimental results show that the experimental group has optimal effect, the treatment effective rate, the cure rate and the time required for curing are all superior to the comparison group lacking the pubescent holly root and the common pellet group lacking the platycodon grandiflorum and the pubescent holly root, and the experimental group can prove that the platycodon grandiflorum, the pubescent holly root and the tilmicosin can take effect synergistically, so that the treatment effect is obviously improved, the treatment time is greatly shortened, and the experimental group can be possibly related to the effect that the pubescent holly root and the platycodon grandiflorum can cooperate to promote the radix platycodonis to have the effect of guiding drugs to the channels. Compared with the conventional medicine group, the two groups have better treatment effective rate, which is probably related to the following factors, after coating, 1) the feed intake is improved, the factors that some pigs do not take feed due to the addition of tilmicosin are reduced, 2) the medicine irritation and degradation phenomena caused by the release of the tilmicosin in the stomach are reduced, and the clinical effect is higher than that of the common coated pellet.
The embodiments described above are only preferred embodiments of the invention and are not exhaustive of the possible implementations of the invention. Any obvious modifications to the above would be obvious to those of ordinary skill in the art, but would not bring the invention so modified beyond the spirit and scope of the present invention.
Claims (7)
1. A traditional Chinese medicine targeted tilmicosin pellet is characterized by comprising the following raw materials in parts by weight:
2. the traditional Chinese medicine targeted tilmicosin pellet as claimed in claim 1, wherein the disintegrant is one or both of microcrystalline cellulose and sodium carboxymethylcellulose.
3. The traditional Chinese medicine targeted tilmicosin pellet as claimed in claim 1, wherein the adhesive is one or more of bletilla polysaccharide gum, dextrin and powdered sugar.
4. The traditional Chinese medicine targeted tilmicosin pellet as claimed in claim 1, wherein the filler is one or two selected from astragalus polysaccharides and starch.
5. The preparation method of the traditional Chinese medicine targeted tilmicosin pellet according to claim 1, which is characterized by comprising the following steps:
step 1, respectively crushing platycodon grandiflorum and pubescent holly root by using a jet mill, and sieving with a 350-mesh sieve to obtain superfine platycodon grandiflorum powder and superfine pubescent holly root powder for later use;
step 2, weighing the raw materials in parts by weight, and then mixing the raw materials in a mass ratio of the adhesive to water of 2: 1, dissolving the adhesive by using water, and uniformly stirring to obtain an adhesive aqueous solution;
step 3, stirring tilmicosin, platycodon grandiflorum superfine powder, pubescent holly root superfine powder, disintegrating agent and filler at the rotating speed of 60 revolutions per minute for 10 minutes, then adding the adhesive aqueous solution, and continuously stirring for 30 minutes to prepare a soft material;
step 4, granulating, shot blasting, drying and coating the prepared soft material to obtain pellets;
the adhesive is bletilla striata polysaccharide gum;
the disintegrating agent adopts microcrystalline cellulose;
the filler is astragalus polysaccharide.
6. The method for preparing a traditional Chinese medicine targeted tilmicosin pellet as claimed in claim 5, wherein the step 4 specifically comprises the following operations:
step 4-1, extruding and granulating the prepared soft material, and sieving with a 40-mesh sieve to obtain medicine granules;
step 4-2, performing shot blasting on the prepared medicine particles by using a shot blasting machine at the speed of 150 revolutions per minute until the pill cores are rounded, performing fluidized drying for 30 minutes, and naturally cooling to 45 ℃ to obtain the pill cores;
and 4-3, firstly dissolving the coating auxiliary materials by using a solvent to prepare a coating solution, then filling the cores into a multifunctional fluidized bed coating machine, and after the temperature of an air outlet is raised to 60 ℃, adding the cores and the coating auxiliary materials according to the mass ratio of the dry weight of the cores to the dry weight of the coating auxiliary materials of 8-15: 1, pumping the coating liquid into a spray gun through a peristaltic pump for spray coating, and keeping the temperature of an air outlet at 65 ℃ in the coating process; and after coating, continuously keeping the coating in a multifunctional fluidized bed coating machine for 15 minutes for drying.
7. The preparation method of the traditional Chinese medicine targeted tilmicosin pellet as claimed in claim 6, wherein the coating solution comprises the following raw materials in parts by weight: 5-10 parts of coating auxiliary materials; solvent: 60 parts of absolute ethyl alcohol;
wherein the coating auxiliary material comprises 3-8 parts of hydroxypropyl methyl cellulose phthalate or polyacrylic resin; 1-3 parts of triethyl citrate; 1-2 parts of talcum powder.
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| WO2015138042A2 (en) * | 2014-03-14 | 2015-09-17 | Bovicor Pharmatech Inc. | Nitric oxide treatment of bovine respiratory disease complex and other respiratory conditions |
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Application publication date: 20190222 Assignee: Beijing Leguan Pet Products Co.,Ltd. Assignor: HEBEI DEPOND ANIMAL HEALTH TECHNOLOGY CO.,LTD. Contract record no.: X2023980036556 Denomination of invention: A Chinese medicine targeted Tilmicosin pellets and its preparation method Granted publication date: 20210402 License type: Common License Record date: 20230613 |
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