CN109364011A - It is a kind of to be dissipated and preparation method thereof so that the coated of whitening spot-removing new eight is white - Google Patents
It is a kind of to be dissipated and preparation method thereof so that the coated of whitening spot-removing new eight is white Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/56—Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Abstract
The invention belongs to cosmetic technical fields, and in particular to a kind of to dissipate and preparation method thereof so that the coated of whitening spot-removing new eight is white.The coated new eight is white to be dissipated and mainly prepares raw material and be made of the larva of a silkworm with batrytis, tribulus terrestris, the bletilla striata, PORIA ALBA, the root bark of white mulberry, radix ampelopsis, Radix Paeoniae Alba and each 10 mass parts of pearl powder.The present invention is different according to each ingredient active cell signal path, coordinated regulation tyrosinase activity, and can sufficiently be allowed to be embedded in liposome and play activity, is by the combination of traditional activated substance and Modern preparations technology.It dissipates with certain stimulation and may cause allergic reaction, and good white-skinned face function the present invention overcomes tradition eight is white.
Description
Technical field
The invention belongs to cosmetic technical fields, and in particular to it is a kind of can with the coated of whitening spot-removing new eight it is white dissipate and its
Preparation method.
Background technique
The color of normal human subject skin depends primarily on the content and distribution of melanin.Melanin is closed in melanocyte
At, it generates and is mainly regulated and controled by tyrosinase activity.Tyrosinase activity increases, and the synthetic quantity of melanin just increases, instead
It, the production quantity of melanin will be reduced significantly.The disorder of tyrosinase increases and can cause Pigmentosus disease such as chloasma, sparrow
Spot, foxiness, blackspot, senile plaque even melanocytoma etc..The effect of tyrosine in melanocyte in enzymes such as tyrosinases
Under be gradually oxidized to melanin.In recent years, it has developed inhibition in succession both at home and abroad or blocks the skin beauty of tyrosinase activity
White dose, such as arbutin, kojic acid, oil soluble licorice extract, four different palmitates.But be single use any whitening at
Point, all cannot completely whitening effect.Combination compound action whitening agent have become current whitening product Formula Development trend it
One.
Chinese Traditional Medicine is the rarity of Chinese traditional culture of long standing and well established.Wherein it is no lack of for women beauty and whitening
Lonely virtue, if the ancient prescription " eight is white scattered " in Jin Dynasty imperial palace is more representative " beauty in the numerous skin maintenance and cosmetic benefits prescriptions of Traditional Chinese Medicine
It is white " recipe.Recorded according in " Yifangleiju ", eight white scattered formulas are as follows: white lilac, the larva of a silkworm with batrytis, rhizoma typhonii, it is white lead a cow, the root of Dahurain angelica,
Tribulus terrestris, PORIA ALBA, the bletilla striata.It commonly uses this side to wash one's face, can there is the effect for making face keep delicate as white snow." traditional Chinese medicine beautifying is complete works of "
Medicinal material used in the exterior-applied prescription for the treatment facial colour spot that one book is included is analyzed, the root of Dahurain angelica used in the side of learning, rhizoma typhonii, white
Bombyx batryticatus is really successive dynasties beauty ancient prescription key medicine.Traditional ancient prescription is also required to the evaluation result for carrying out safety and cosmetics regulation increasingly
It is perfect, have 4 taste Chinese medicines in " eight white dissipate " because there is certain stimulation and may cause allergic reaction and cannot use.
Shennong's Herbal, which carries the larva of a silkworm with batrytis, the effect of " go out blackspot, and it is good to make us complexion ".Its active constituent amino acid and work
Sex pilus light element, nutritious skin and beautification function, are beneficial to blood circulation promoting and enriching and beautifying face and moistering lotion.Tribulus terrestris is soothing the liver with wind-dispelling,
The promoting flow of qi and blood circulation and antipruritic effect, peroxide catabolic enzyme contained therein have significant anti-aging effects, and make skin soft
Tender lubrication has beauty functions；Three plain soups recorded derived from the medical literature " Elementary Medicine " of the Ming Dynasty: Radix Paeoniae Alba, Rhizoma Atractylodis Macrocephalae, PORIA ALBA
It is aided with Radix Glycyrrhizae decocting, warmly takes, suitable for pachylosis caused by the qi and blood cold of insufficiency type, sallow, chloasma, pigmentation etc..Radix ampelopsis is Portugal
The root of grape section plant radix ampelopsis has extraordinary whitening function, whitening, treatment facial freckle, heat-clearing wind-dispelling.Radix ampelopsis is to whitening flesh
Skin has special efficacy, and " haigoushen " is recorded: " can control sore blister on face.".The seven white whitening mask of son be by Rhizoma Atractylodis Macrocephalae, the root of Dahurain angelica, the bletilla striata, radix ampelopsis,
PORIA ALBA, six kinds of pure Chinese medicine powder of Radix Paeoniae Alba and pearl powder are mixed manufactured whitening mask.For eliminating skin speckle, eliminating skin
Skin small pox has the effect got instant result.The root bark of white mulberry is good tyrosinase inhibitor in Moraceae Chinese medicine, in skin-whitening and
Preserving fruit and vegetable utilizing etc. has potential application.Radix Paeoniae Alba is inhibited to tyrosinase.The bletilla striata is famous in ancient
One of the ingredient of whitening prescription " eight white dissipate ", Huang Hao etc. ' takes water as a solvent, and with effective component in the ultrasonic extraction bletilla striata, and verifies
Bletilla striata extract inhibits the ability of tyrosinase, and value is experimental research suggested, and the bletilla striata has good white-skinned face function, verifying
The reasonability of ancient prescription.
However, the effect of traditional traditional Chinese medicine ingredients is relatively slow, and effect is unobvious, and allergy and skin even occurs in part
The reaction such as discomfort.The effectively absorption of its active constituent is urgently to be resolved.
Technique for packing of the liposome technology characterized by its unique control release (sustained release) effect is in Chinese medicine beauty
It is had been more and more widely used in prescription and cosmetic industry.It is by Chinese prescription drug carry out reasonable drawing after,
It is handled with liposome technology.On the one hand, the function of liposome is as inert carrier, and due to cutin
The membrane structure of lipid structures and liposome is closely similar in layer, all has double film characters, active component can be transported to epidermis
Layer.On the other hand, the package of liposome is effectively protected active Chinese drug component extract, in use, according to its sustained release
Extract is gradually released in skin according to preparatory desired rate and works by effect.Various lipids and lipid mixture are equal
It can be used for preparing liposome, and phosphatide is most common.Such as from organism lecithin, serinephosphatide and sphingomyelin
And dipalmitoylphosphatidylcholine, Distearoyl Phosphatidylcholine of synthesis etc..Some researches show that ceramide lipids at present
Body is the barrier system of most natural, ideal Stratum corneum lipids, it is not only able to satisfy the needs of lipid barrier, moreover it is possible to bring skin
The careful smooth appearance of skin.
It is currently used to have conventional liposome, Deformable liposome, alcohol liposome etc..(also known as lipid is double for conventional liposome
Tunic vesicle), it is the hollow spheres body being bundled by bimolecular lamellar lipid membrane, is allowed to smoothly penetrate skin.The base of conventional liposome
Be added on plinth appropriate sodium taurocholate and 3%-7% ethyl alcohol or propylene glycol made from deformable liposome, partial size is smaller than liposome,
Transmission rates and quantity are almost suitable with pure water.Alcohol liposome, which refers to, has rouge for what phosphatide, high concentration ethanol and water were prepared into
The preparation intermediate of matter Bilayer vesicle structure.Liang Zhenye etc. prepares cinnamomum cassia extract alcohol plastid using injection method, by alcohol matter
Body and various concentration hydroxypropyl methyl cellulose (HPMC) gel are mixed with cinnamomum cassia extract gel, 2%HPMC concentration conduct
Transdermal penetration rates highest when cinnamomum cassia extract alcohol plastid matrix.So East Airways etc. is prepared for the beta-cyclodextrin packet of cinnamomum cassia extract
Object is closed, alcohol plastid is made using injection method.Compared with inclusion compound aqueous solution, the infiltration rate of alcohol plastid is significantly increased.
Function of the liposome played in cosmetics is summed up as follows: antioxidant, emulsifier, moisturizer, tune
Manage agent, softening and emollient, bleeding agent, stabilizer, nutritional supplement, vitamin source.Just because of these excellent properties, lipid
Application of the body in cosmetics has caused the very big concern of people, existing more than 100 novel makeups containing liposome American-European so far
Product listing.Currently, the product using liposome embedded active material mainly has: embedding beta carotene, flavonoids, superoxides
Mutase (SOD), Co-Q10 and vitamin E and its derivative etc..
Liposome is more promising permeating method, but its restriction by preparation technique, when in conjunction with Chinese medicine
It need to consider the stability problem such as liposome, the drugloading rate of liposome, encapsulation rate problem, and how extend liposome and enter people
Retention time and problem encountered in production after body.
Summary of the invention
To solve the problems, such as that existing whitening and freckle-removing product exists in use process, the primary purpose of the present invention is that providing one
Kind can be dissipated so that the coated of whitening spot-removing new eight is white.The coated new eight is white dissipate have stimulate it is small, do not cause allergic reaction, property
Stablize, bioavilability is high, is easily absorbed by the skin, the features such as whitening spot-removing effect is good.
Another object of the present invention is to provide above-mentioned one kind can be with the white scattered preparation side of the coated of whitening spot-removing new eight
Method.The present invention improves traditional eight white dissipate and by liposome technical application whitening spot-removing class cosmetics, will in
Medicine group formula extraction with technique for packing is organic is reasonably combined together, ancient prescription the present is used, and new eight white scattered properties can be made more steady
It is fixed, bioavilability is high, be easily absorbed by the skin, whitening spot-removing effect is more preferable.
The object of the invention is achieved through the following technical solutions:
It is a kind of to be dissipated so that the coated of whitening spot-removing new eight is white, mainly prepare raw material by the larva of a silkworm with batrytis, tribulus terrestris, the bletilla striata,
PORIA ALBA, the root bark of white mulberry, radix ampelopsis, Radix Paeoniae Alba and pearl powder composition are the larva of a silkworm with batrytis, tribulus terrestris, the bletilla striata, PORIA ALBA, the root bark of white mulberry, white
The mass fraction of liana, Radix Paeoniae Alba and pearl powder is 10 mass parts.
It can mainly be used inverse with the white scattered preparation method of the coated of whitening spot-removing new eight the present invention also provides above-mentioned
Phase evaporation prepares that coated new eight is white to be dissipated, and includes the following steps:
(1) larva of a silkworm with batrytis, tribulus terrestris, the bletilla striata, PORIA ALBA, the root bark of white mulberry, radix ampelopsis, Radix Paeoniae Alba and each 10 mass parts of pearl powder are weighed,
35% ethyl alcohol, 50% ethyl alcohol, 65% ethyl alcohol, 80% ethyl alcohol, 95% ethyl alcohol, dehydrated alcohol is successively used to carry out as Extraction solvent
It extracts, the Extraction solvent amount used every time is 100 parts by volume, is extracted 4 hours every time；Combined extract after the completion of extraction, water-bath
It is heated at reflux 1h, the medical fluid for being prepared into 10 parts by volume is concentrated under reduced pressure in filtering；
(2) according to lecithin: cholesterol=4:1 mass ratio weighs a certain amount of lecithin and cholesterol in reaction
In container, chloroform is added and makes it dissolve, obtains oily phase；
(3) medical fluid made from (1) is added into oil phase made from step (2) and carries out ultrasonication, the lipid made
Body is small and uniform, forms stable w/o type emulsion；
(4) organic solvent removed in w/o type emulsion is then evaporated under reduced pressure, after reaching colloidal state, phosphate buffer is added dropwise,
So that the gel on wall is fallen off by the rotation of rotary evaporator, then proceedes to be evaporated under reduced pressure, after organic solvent completely removes
Mechanical concussion is carried out, keeps gum dispersion uniform, aqueous suspension is made；
(5) non-encapsulated drug is removed by exclusion chromatography or supercentrifugation, obtaining that liposome is i.e. described can beauty
The coated of white nti-freckle new eight is white to be dissipated.
The present invention solves the larva of a silkworm with batrytis, tribulus terrestris, the bletilla striata, PORIA ALBA, the root bark of white mulberry, radix ampelopsis, Radix Paeoniae Alba and pearl powder in lipid
The drugloading rate of stability, liposome in body, encapsulation rate, the problems such as organically blending of 8 kinds of ingredients, gained liposome has preferable
Stability, and have whitening timid spot activity.
Chloroform dosage does not need to be particularly limited in above-mentioned steps (2), and lecithin and cholesterol are completely dissolved.Step
(3) oil mutually does not need to be particularly limited to the ratio of medical fluid in, and removing organic solvents, chloroform, step (5) can be evaporated under reduced pressure in step (4)
Non-encapsulated drug can be removed.Therefore guarantee prepares the mass ratio of lecithin and cholesterol during liposome.In order to
Facilitate operation, oil is preferably mutually 3:1 with the volume ratio of medical fluid.
Preferably, the ultrasonication refers to that in ultrasound intensity be 40%, in the case where working time 5s, interval 10s
Ultrasound 10 times.
Preferably, the temperature of the reduction vaporization is 30 DEG C.
Preferably, it is 0.01mol/L, pH=6.5 phosphate buffer that the buffer, which is concentration, and dripping quantity is 40 volumes
Part.
1 mass parts of the present invention: 1 parts by volume=1g/mL.
35% ethyl alcohol of the present invention, 50% ethyl alcohol, 65% ethyl alcohol, 80% ethyl alcohol, 95% ethyl alcohol are percentage by volume.
The tyrosinase inhibitor extracted in the white scattered component natural products of coated new eight provided by the invention contains carboxylic more
Base, hydroxyl isopolarity group, selecting the ethyl alcohol of different volumes score is Extraction solvent, selects optimal combination object proportion, fixed material
Liquor ratio and other extraction conditions, using tyrosinase inhibition rate and DPPH free radical scavenging activity as evaluation index.
The extracting solution of processing is not wrapped the present invention also provides the extract Jing Guo liposome and to tyrosinase
Inhibition, the comparative experiments to skin whitening effect and the whitening effect to skin clinical trial further verify the package
Type new eight is white dissipate easily be absorbed by the skin, the features such as whitening spot-removing effect is more preferable.
Compared with prior art, the present invention has the following advantages and beneficial effects:
(1) coated provided by the invention new eight it is white dissipate by the larva of a silkworm with batrytis, tribulus terrestris, the bletilla striata, PORIA ALBA, the root bark of white mulberry, radix ampelopsis,
Radix Paeoniae Alba, pearl powder are made, and are retaining the traditional eight white scattered larva of a silkworm with batrytis, tribulus terrestris, the bletilla striata, PORIA ALBA, have discarded white lilac, white attached
It is sub, white lead a cow, the root of Dahurain angelica, increase three kinds of ingredients such as radix ampelopsis, the root bark of white mulberry, Radix Paeoniae Alba, pearl powder.Overcoming white dissipate of tradition eight has one
Fixed stimulation and it may cause allergic reaction, and good white-skinned face function.
(2) present invention by Traditional Chinese medicine formula extract with technique for packing is organic is reasonably combined together, ancient prescription the present use can be with
Make that new eight white scattered properties are more stable, bioavilability is high, be easily absorbed by the skin, whitening spot-removing is imitated.
(3) present invention is sufficiently different according to each ingredient active cell signal path during selecting prescription, and collaboration is adjusted
Tyrosinase activity is controlled, and can sufficiently be allowed to be embedded in liposome and play activity, is by traditional activated substance and to show
For the combination of preparation technique.
Detailed description of the invention
Fig. 1 is the extract of liposome and comparison reality of the extract to skin chromatic aberration for not being wrapped processing
It tests.
The extract of Fig. 2 liposome compares dermal melanin changes of contents with the extract for not being wrapped processing real
It tests.
In above-mentioned attached drawing: the liposome type new eight that coated " new eight is white scattered " extract refers to prepared by embodiment 1 is white
It dissipates, does not wrap up " new eight is white scattered " extract and refer to new eight white scattered extracting solutions prepared by embodiment 1.
Specific embodiment
Below with reference to embodiment and attached drawing, the present invention is described in further detail, but embodiments of the present invention are unlimited
In this.
Embodiment 1
(1) preparation of new eight white scattered extracting solutions: the tyrosinase inhibitor extracted in natural products contains carboxyl, hydroxyl more
Isopolarity group, selecting the ethyl alcohol of different volumes score is Extraction solvent, selects optimal combination object proportion, fixed solid-liquid ratio and its
His extraction conditions, using tyrosinase inhibition rate and DPPH free radical scavenging activity as evaluation index.With optimal proportion weigh the larva of a silkworm with batrytis,
Tribulus terrestris, the bletilla striata, PORIA ALBA, the root bark of white mulberry, radix ampelopsis, Radix Paeoniae Alba and each 10g of pearl powder, successively use 35% ethyl alcohol, 50% by totally 8 parts
Ethyl alcohol, 65% ethyl alcohol, 80% ethyl alcohol, 95% ethyl alcohol, dehydrated alcohol are extracted as Extraction solvent, and the extraction used every time is molten
Dosage is 100 parts by volume, is extracted 4 hours every time；Combined extract after the completion of extraction, water-bath are heated at reflux 1h, and filtering decompression is dense
Contracting is prepared into the medical fluid (i.e. new eight white scattered extracting solution) of 10mL.
(2) inhibit tyrosinase Bush Vitality's: taking medical fluid made from 1mL step (1), tyrosinase is not added, medical fluid is set
10min is kept the temperature in 37 DEG C of water-baths；Medical fluid made from 1mL step (1) is taken, tyrosinase is added, medical fluid is placed in 37 DEG C of water
Constant temperature 10min in bath；Measuring absorbance measurement at 475nm, it inhibits tyrosinase vigor.
(3) DPPH radicals scavenging is tested: accurately pipetting reaction solution in 2mL DPPH solution in centrifuge tube, 2mL is added to walk
Suddenly medical fluid made from (1) is uniformly mixed, is protected from light arrest reaction 30min, absorbance is measured at 517nm, using ethyl alcohol as blank
Control.
The white scattered extracting solution of table 1 new eight inhibits tyrosinase vigor and DPPH radicals scavenging Bush Vitality's
| Group | Tyrosinase inhibition rate (%) | DPPH radicals scavenging (%) |
| New eight white scattered extracting solution | 26.5±4.5 | 84.6±3.5 |
(4) the white scattered preparation of liposome type new eight.According to lecithin: gallbladder consolidates the mass ratio of liquor-saturated=4:1, and precision claims
It takes a certain amount of lecithin and cholesterol in pear shape bottle, chloroform is added and makes it dissolve, obtains oily phase；(1) is added into oily phase
Medical fluid obtained carries out ultrasonication in short-term, and (ultrasonication in short-term refers to that in ultrasound intensity be 40%, the working time
Ultrasound 10 times in the case where 5s, interval 10s), wherein oil is mutually 3:1 with the volume ratio of medical fluid, the liposome made is small and equal
It is even, form stable w/o type emulsion；Then 30 DEG C of evaporating organic solvents are depressurized, after reaching colloidal state, dropwise addition 0.01mol/L,
The phosphate buffer 40mL of pH=6.5 helps the gel on wall to fall off, and then continues to evaporate under reduced pressure, is made aqueous mixed
Suspension；Non-encapsulated drug is removed by exclusion chromatography or supercentrifugation, obtains the white scattered i.e. sheet of liposome type new eight
Invention is described to be dissipated so that the coated of whitening spot-removing new eight is white.
The white scattered extract of coated described in embodiment 2 and 3 new eight refers to liposome type new eight prepared by embodiment 1
White to dissipate, the extract for not being wrapped processing refers to new eight white scattered extracting solution made from embodiment 1.
Embodiment 2
The comparative experiments of the white scattered extract of coated new eight and the extract for not being wrapped processing.
1. the white scattered extract of coated new eight and inhibition comparative experiments of the extract to tyrosinase for not being wrapped processing:
The new eight white scattered extracts for being the white scattered extract of 8 μ g/mL coated new eight with mass concentration and not wrapping up, it is molten with l-tyrosine
Liquid (1.5mmol/L) makees substrate, is that 1.42mmol/L vitamin C compares product with concentration, measures the white scattered extraction of coated new eight
Object and the new eight white scattered extracts not wrapped up are to the active inhibiting rate of tyrosine monophenolase.
With new eight white scattered extracting solutions of same mass concentration and the white scattered extract of coated new eight, with L-3,4 dihydroxyphenylalanine solution
(1.5mmol/L) makees substrate, and the vitamin C with concentration for 1.42mmol/L compares product, measures the white scattered extraction of coated new eight
Object is with the new eight white scattered extracting solutions that do not wrap up to the inhibiting rate of tyrosinase diphenolase.It the results are shown in Table 2:
Extract of the table 2 Jing Guo liposome and it is not wrapped inhibition pair of the extract of processing to tyrosinase vigor
Than
| Group | Tyrosine single phenol enzyme inhibition rate (%) | Tyrosine diphenol enzyme inhibition rate (%) |
| The white scattered extract of coated new eight | 89±2.46 | 45±5 |
| The white scattered extracting solution of new eight not wrapped up | 57±3.13 | 60±5 |
| Vc | 88.92±1.31 | 37.12±1.35 |
By table 2, the experimental results showed that, coated new eight provided by the invention is white to dissipate the enzyme activity that can obviously inhibit tyrosinase
Power, the inhibiting effect of the extract of liposome are better than not being wrapped the extract of processing.
2. the white scattered extract of coated new eight and comparative experiments of the extract to skin whitening for not being wrapped processing: this reality
Two set products of setting are tested, one group is the white scattered extract of coated new eight, and another group is the extract for not being wrapped processing.Two kinds of systems
Agent uses in the case where any cosmetics of no smearing, shares 20 volunteers and has participated in this activity.Twice daily, amount to
28 days, the pale degree of skin was to measure skin using colorimeter system as defined in International Commission on Illumination (CIE) (Lab colorimeter system)
The method of the variation of skin color measures.The result is shown in Figure 1.
As shown in Figure 1, white dissipate of coated provided by the invention new eight can be obviously improved skin brightness, with pushing away for time
It moves, skin brightness is stepped up, and the skin whitening effect of the white scattered extract of coated new eight, which is substantially better than, is not wrapped processing
Extract.
3. the white clinical test dissipated to the whitening effect of skin of coated new eight: this experiment shares 10 volunteers and participates in survey
Examination, the age 20~35 years old, male 5, women 5.Volunteer's exclusion condition meet " cosmetics contact dermatitis diagnostic criteria and
Treatment principle " it is included in, exclusion criteria.Daily sooner or later respectively using 1 cosmetics containing the extract by package, during experiment
Subject cannot smear any cosmetics in Test sites.Questionnaire access and the full face analysis of VISIA are carried out after 8 weeks and melanin is surveyed
Determine the method that instrument combines and evaluates its whitening effect.As a result such as Fig. 2.
As shown in Figure 2, white dissipate of coated provided by the invention new eight can obviously reduce dermal melanin content, and with when
Between passage, melanin content gradually reduces.And the skin of the extract of its liposome reduces dermal melanin content
Ability and whitening effect are substantially better than the extract for not being wrapped processing.
Embodiment 3
The white scattered estimation of stability of coated new eight
1, heat-resistant stability is evaluated
The white scattered extract of coated new eight prepared by embodiment 1 is placed respectively with the extract for not being wrapped processing respectively
It in Clear glass bottles and jars, is placed in 48 DEG C of constant temperature experiments, every the appearance character of 3 days observation primary productions.
The results showed that not being wrapped the extract of processing at 8 days, product color starts to become micro- Huang from white
Color, and color is gradually deepened as time goes by；The white scattered extract of coated new eight is observed continuously 25 days, the color of product according to
White is so kept, does not occur the phenomenon that color change.This explanation, after liposome, whitening spot-removing ingredient it is resistance to
Thermal stability increases substantially.
2, light fastness stability is evaluated
The white scattered extract of coated new eight prepared by embodiment 1 is placed respectively with the extract for not being wrapped processing respectively
It in Clear glass bottles and jars, is placed at solar exposure, every the appearance character of 1 day observation primary production.
The results showed that not being wrapped the extract of processing at the 7th day, product color starts to become micro- Huang from white
Color, and color is gradually deepened as time goes by；And the white scattered extract of coated new eight is when being observed continuously the 21st day, product color
Start to become yellowish from white.This explanation, after liposome, the light fastness stability of whitening spot-removing ingredient is substantially mentioned
It is high.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (5)
1. one kind can be dissipated so that the coated of whitening spot-removing new eight is white, which is characterized in that it mainly prepares raw material by the larva of a silkworm with batrytis, white puncture
Chenopodiaceae, the bletilla striata, PORIA ALBA, the root bark of white mulberry, radix ampelopsis, Radix Paeoniae Alba and pearl powder composition, the larva of a silkworm with batrytis, tribulus terrestris, the bletilla striata, PORIA ALBA, mulberry
White skin, radix ampelopsis, Radix Paeoniae Alba and pearl powder mass fraction be 10 mass parts.
2. described in claim 1 can be with the white scattered preparation method of the coated of whitening spot-removing new eight, which is characterized in that including such as
Lower step:
(1) larva of a silkworm with batrytis, tribulus terrestris, the bletilla striata, PORIA ALBA, the root bark of white mulberry, radix ampelopsis, Radix Paeoniae Alba and each 10 mass parts of pearl powder are weighed, successively
35% ethyl alcohol, 50% ethyl alcohol, 65% ethyl alcohol, 80% ethyl alcohol, 95% ethyl alcohol and dehydrated alcohol is used to be mentioned as Extraction solvent
It takes, the Extraction solvent amount used every time is 100 parts by volume, is extracted 4 hours every time；Combined extract after the completion of extraction, water-bath are returned
The medical fluid for being prepared into 10 parts by volume is concentrated under reduced pressure in stream heating 1h, filtering；
(2) according to lecithin: cholesterol=4:1 mass ratio weighs a certain amount of lecithin and cholesterol in reaction vessel
In, chloroform is added and makes it dissolve, obtains oily phase；
(3) medical fluid made from (1) is added into oil phase made from step (2) and carries out ultrasonication, form stable w/o type cream
Agent；
(4) organic solvent removed in w/o type emulsion is then evaporated under reduced pressure, after reaching colloidal state, phosphate buffer is added dropwise, passes through
The rotation of rotary evaporator makes the gel on wall fall off, and then proceedes to be evaporated under reduced pressure, carry out after organic solvent completely removes
Machinery concussion, keeps gum dispersion uniform, and aqueous suspension is made；
(5) non-encapsulated drug is removed by exclusion chromatography or supercentrifugation, obtain liposome i.e. described in can be dispelled with whitening
The coated of spot new eight is white to be dissipated.
3. according to claim 2 can be with the white scattered preparation method of the coated of whitening spot-removing new eight, which is characterized in that institute
It states ultrasonication and refers to that in ultrasound intensity be 40%, ultrasound 10 times in the case where working time 5s, interval 10s.
4. according to claim 2 can be with the white scattered preparation method of the coated of whitening spot-removing new eight, which is characterized in that institute
The temperature for stating reduction vaporization is 30 DEG C.
5. according to claim 2 can be with the white scattered preparation method of the coated of whitening spot-removing new eight, which is characterized in that institute
It is 0.01mol/L, pH=6.5 phosphate buffer that state phosphate buffer, which be concentration, and dripping quantity is 40 parts by volume.
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| CN201811458713.7A CN109364011A (en) | 2018-11-30 | 2018-11-30 | It is a kind of to be dissipated and preparation method thereof so that the coated of whitening spot-removing new eight is white |
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| CN201811458713.7A CN109364011A (en) | 2018-11-30 | 2018-11-30 | It is a kind of to be dissipated and preparation method thereof so that the coated of whitening spot-removing new eight is white |
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| CN112972607A (en) * | 2021-02-04 | 2021-06-18 | 深圳一支蓝生物科技有限公司 | Pearl and white jade powder for whitening skin and removing freckles and preparation method thereof |
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