CN1093634C - Quick-acting ceresin-wax slicing method - Google Patents
Quick-acting ceresin-wax slicing method Download PDFInfo
- Publication number
- CN1093634C CN1093634C CN 97119457 CN97119457A CN1093634C CN 1093634 C CN1093634 C CN 1093634C CN 97119457 CN97119457 CN 97119457 CN 97119457 A CN97119457 A CN 97119457A CN 1093634 C CN1093634 C CN 1093634C
- Authority
- CN
- China
- Prior art keywords
- section
- minutes
- paraffin
- dewatering
- alcohol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention discloses a quick paraffin section method which comprises the steps of materials taking, fixing, dewatering, hyalinizing, paraffin soaking, section cutting, section baking, paraffin removing, all-level alcohol and water washing, dyeing, dewatering, hyalinizing, sealing, etc. The method is characterized in that an AF solution is used for fixing, and the soaking time is from 50 minutes to 60 minutes; the dewatering process is carried out twice respectively by 95% alcohol and acetone stock solution, and the dewatering time is controlled between 20 minutes and 30 minutes each time; the temperature of paraffin soaking is between 70 DEG C and 80 DEG C. Compared with the common paraffin section method, the present invention is capable of improving the speed of section preparation for more than 5 times and ensuring the quality of section preparation, and obtains precious time for clinical diagnosis and patients in emergency.
Description
The present invention relates to a kind of method of quick preparing human tissue pathological section.
Thereby common paraffin section method also is to adopt fixing, dehydration, transparent, waxdip, section to obtain paraffin section, but grows to long that time of making pathological diagnosis drags because the step times spent such as fixing, dehydration, waxdip cross, and often just can go out the result in three days.
The purpose of this invention is to provide a kind of method efficiently,, improve film-making speed, reach the purpose that goes out the result soon by to the improvement on the common paraffin section method step.
Technical scheme of the present invention is as follows:
By draw materials, fix, steps such as dehydration, transparent, waxdip, section, roasting sheet, dewaxing, ethanol at different levels and washing, dyeing, dehydration, transparent, sealing form, be characterized in: material fixedly is employing AF liquid immersion 50-60 minute; The dehydration of carrying out after fixing adopts 95% alcohol and acetone stoste to carry out at twice, each 20-30 of dewatering time minute; 70-80 ℃ of waxdip temperature.
Advantage of the present invention is to guarantee can to improve film-making speed more than 5 times under the slice-making quality prerequisite identical with common paraffin section method.A pathology needs three order of classes or grades at school usually, and this method just can be finished in four hours, was clinical diagnosis and first-aid patients acquisition quality time.
Embodiment: get biological tissue 0.1-1cm
2Carry out following step process
Step solution and other require the time
Fixedly anhydrous alcohol is 90 milliliters and 1 hour
10 milliliters of mixing of formalin
95% alcohol 30 minutes dewaters
Acetone stoste 30 minutes
Transparent dimethylbenzene stoste 10 minutes
75 ℃ of waxdip paraffin temperature 30 minutes
4 microns of section slice thicknesses
65 ℃ of 15 minutes dewaxing dimethylbenzene of roasting sheet oven temperature 11 minutes
2 30 seconds alcohol of dimethylbenzene was washed anhydrous alcohol 10 seconds
95% alcohol 10 seconds
80% alcohol washing in 10 seconds tap water 1 minute
Aquae destillata dyeing in 10 seconds was breathed out Rui Shi haematine dye liquor 2 minutes
Tap water 30 seconds
1% hydrochloride alcohol several seconds
Tap water 1 minute
Unsaturated carbonate lithium solution 20 seconds
Tap water 2 minutes
Eosin stain dehydration in 2 minutes 80% alcohol 10 seconds
95% alcohol 10 seconds
Anhydrous alcohol 10 seconds
30 seconds transparent dimethylbenzene of anhydrous alcohol 30 seconds
30 seconds sealing neutral gums of dimethylbenzene
Claims (1)
1, quick-acting ceresin-wax slicing method, by draw materials, fix, dehydration, transparent, waxdip, section, roasting sheet, dewaxing, ethanol at different levels and washing, dyeing, dehydration, transparent, sealing step form, it is characterized in that: fixing employing AF liquid, soak time 50-60 minute; Be connected on the fixedly dehydration of back and adopt 95% alcohol and acetone stoste subnetting to carry out, dewatering time respectively was controlled at 20-30 minute; 70-80 ℃ of waxdip temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97119457 CN1093634C (en) | 1997-11-09 | 1997-11-09 | Quick-acting ceresin-wax slicing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97119457 CN1093634C (en) | 1997-11-09 | 1997-11-09 | Quick-acting ceresin-wax slicing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1217468A CN1217468A (en) | 1999-05-26 |
| CN1093634C true CN1093634C (en) | 2002-10-30 |
Family
ID=5175388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 97119457 Expired - Fee Related CN1093634C (en) | 1997-11-09 | 1997-11-09 | Quick-acting ceresin-wax slicing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1093634C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100460850C (en) * | 2005-07-20 | 2009-02-11 | 徐华 | Making method of body fluid cell lump paraffin section |
| CN102116711B (en) * | 2011-01-31 | 2012-11-21 | 山东东方海洋科技股份有限公司 | Manufacturing method of paraffin sections of zostera marina embryo |
| CN102313660A (en) * | 2011-10-09 | 2012-01-11 | 中国科学院南海海洋研究所 | Manufacturing method of paraffin section of hermatypic coral oocyte |
| CN103954484B (en) * | 2014-04-02 | 2016-08-24 | 浙江万里学院 | A kind of manufacture method of Trionyx sinensis (Wiegmann) gonad paraffin section in early days |
| CN113277745B (en) * | 2021-06-04 | 2023-07-25 | 蓝思科技（长沙）有限公司 | Fingerprint-proof film coating process and glass |
-
1997
- 1997-11-09 CN CN 97119457 patent/CN1093634C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1217468A (en) | 1999-05-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1190445C (en) | Process for producing functional silk fibroin and utilization of the same | |
| CN103940658A (en) | Method for manufacturing paraffin-embedded tissue cell specimen | |
| CN1093634C (en) | Quick-acting ceresin-wax slicing method | |
| CN113527466B (en) | Preparation method of implant grade II type collagen | |
| DE2422737C3 (en) | Process for the preparation of the D-amino acids D - (-) - valine and D - (-) - alanine | |
| CN106092677B (en) | Sweet potato and its relative genus plant cell division phases sample fast preparation method | |
| Foot | Chemical contrasts between collagenous and reticular connective tissue | |
| CN109323907B (en) | Kit for rapidly decalcifying osteochondral tissue and preparation method thereof | |
| CN105973663A (en) | Production method of colorful bone sample | |
| EP0998971A3 (en) | Process for treating aqueous solutions containing bases and organic acids | |
| CN110672395A (en) | HE staining method for tissue section | |
| CN111939321B (en) | Preparation method of pig acellular dermal matrix skin substitute | |
| CN116358950A (en) | Rapid dehydration and transparency method for pathological section | |
| CN114088493A (en) | Animal eyeball pathological section manufacturing method | |
| CN112284861A (en) | Fixing liquid for hypsizigus marmoreus basidiospore microscopic observation, preparation method, fixing method and application | |
| CN105255852B (en) | Utilize the method for ceramic membrane separation immobilised enzymes and heparin enzymolysis liquid | |
| DE10157182A1 (en) | Process for the treatment of materials of biological origin and elastin product | |
| KR102416674B1 (en) | Method of Extracting alkali-soluble alginate from Brown Seaweed by using surfactant-treatment | |
| CN112067409A (en) | Improved H.E staining method for mouse bone marrow tissue section | |
| CN114225112A (en) | Preparation method of cerebrospinal fluid isolation repairing film | |
| CN111826336A (en) | Method for extracting gutta-percha cells from eucommia ulmoides | |
| CN107602692A (en) | A kind of preparation method of collagen diaphragm material | |
| JPH01298272A (en) | Method for scouring and bleaching cotton knit fabric | |
| CN114085806A (en) | Method for extracting and separating dried amnion from placenta | |
| CN1419861A (en) | Aloe gel juice degradation clarifying method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |