CN109363238A - A kind of soft quasi- carbonization ferment particulate of fire pockmarks, preparation method and the usage - Google Patents
A kind of soft quasi- carbonization ferment particulate of fire pockmarks, preparation method and the usage Download PDFInfo
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24D—CIGARS; CIGARETTES; TOBACCO SMOKE FILTERS; MOUTHPIECES FOR CIGARS OR CIGARETTES; MANUFACTURE OF TOBACCO SMOKE FILTERS OR MOUTHPIECES
- A24D3/00—Tobacco smoke filters, e.g. filter-tips, filtering inserts; Filters specially adapted for simulated smoking devices; Mouthpieces for cigars or cigarettes
- A24D3/06—Use of materials for tobacco smoke filters
- A24D3/14—Use of materials for tobacco smoke filters of organic materials as additive
- A24D3/145—Microbial metabolite, e.g. enzyme
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Abstract
The invention discloses a kind of soft quasi- carbonization ferment particulates of fiery pockmarks, the particle is to carry out heating soft quasi- carbonization treatment as raw material using fiery pockmarks, then it is fermented by bacillus subtilis Van3 to it, it is 100~180 mesh powders that the intermediate product after fermentation, which is ground into partial size, again, it is the particle of 20~60 mesh, the as described fiery soft quasi- carbonization ferment particulate of pockmarks at partial size with adhesive bonding.The invention also discloses the preparation method of the fiery soft quasi- carbonization ferment particulate of pockmarks and for the purposes in cigarette filter tip.The soft quasi- carbonization ferment particulate of fiery pockmarks of the invention can selective absorption cigarette smoke harmful substance, while the special burnt odor taste for remaining fiery pockmarks itself.
Description
Technical field
The present invention relates to cigarette material preparation technical fields, and in particular to a kind of soft quasi- carbonization ferment particulate of fire pockmarks, its
Preparation method and purposes for cigarette filter tip.
Background technique
Low coking has been the inexorable trend of tobacco business development, and low coking bring cigarette smoking mouthfeel reduces, and fragrance is not
Be increasingly becoming tobacco development enough highlights problem.How to capture influences tobacco aroma quality, the key technology of perfume quantity, has become
Where the core for improving production of cigarettes competitiveness.Essence spice for cigarette is added in cigarette shreds, has been a kind of conventional, required
The technological means of fragrance is augmented, but its space for promoting cigarette quality is relatively limited, therefore from the non-burning section of cigarette, it finds
Relevant new material has become new hair come the aroma quality and perfume quantity, reduction cigarette smoke irritation for improving, being promoted cigarette
Exhibition trend.Wherein, some granular materials with porosity and special aroma are added, in cigarette filter rod to improve or be promoted volume
The quality of smoke product is a kind of relatively new, effective research direction.But due in preparation method and raw material selection and group of formula
Face there is also some problems, so that existing granular materials additive advantage fails to give full play of.Such as active carbon, micro mist
Although particle made of the sorptive materials such as silica gel has remarkable efficacy in absorption flue gas harmful substance, perfume (or spice) is also resulted in simultaneously
A large amount of absorption of taste ingredient, so that cigarette smoking quality significantly reduces.And the plants particles material obtained by granulation, although
The fragrance matter mutually coordinated with cigarette flavor can be introduced into cigarette smoke, but often because the addition of adhesive, causes to plant
Object material hole itself is embedded, and reduces the adsorption site of harmful substance, reduces reducing tar and reducing harm effect.In addition, for original
The selection and processing of material, the harmony of each plant material and cigarette flavor that the prior art is announced is it is still necessary to be promoted, tobacco sheet
It is fragrant abundant not enough, it need to be improved.
For disadvantages mentioned above, how to select appropriate material of vegetable origin and be modified, to make up prior art technology
Deficiency, reducing, cigarette smoke is irritating simultaneously, improves granular materials and is increasing cigarette smoke harmony and the side such as sense of promoting the production of body fluid
The effect in face just becomes and needs the technical issues of further solving.
Plant heats soft quasi- carbonization, i.e., by dry plant at relatively high temperatures (general 100~300 DEG C, preferably 120~
200 DEG C) it part inside plant cell is sloughed (within general 20h, preferably 2~8h) in a short time combines water, in plant
Portion forms gap, which can adsorb flue gas harmful substance.Plant heats soft quasi- carbonization technique temperature and is not achieved simultaneously
Plant carbide temperature, the technique do not destroy the aromatic property of plant itself.
It is converted using the extraction that microbial fermentation technology carries out fragrance, is widely used in flavouring essence for tobacco.It is micro-
Biology can produce huge high activity enzyme system in breeding, in the metabolism of enzymatic catalysis, chemical action and microorganism complexity
Under synergistic effect, the low molecular compound of large amount of complex can be formed, including various aroma substances, these aroma substances can be made
For the aroma component of tobacco additive agent, strengthens cigarette perfume feature, improves mouthfeel.It is former to have odorous plant to itself using microorganism
Material ferments, moreover it is possible to assign material of vegetable origin to be different from former odorous unique perfume.
Summary of the invention
The present invention is for flue gas noxious substance effect existing for existing plant granule is unobvious and absorbing material granules
The disadvantages of fragrant and cigarette flavor harmony is bad cannot be directly assigned, by being modified to fiery pockmarks raw material, provided one kind both
The fire fiber crops that lowering harm and decreasing coking effect can be enhanced, reduce cigarette smoke irritation, and can enhance and assign fragrant effect, sweet sense can be obviously improved
Soft quasi- carbonization ferment particulate of son and preparation method thereof, and the particle is used in cigarette filter tip, the special coke of fiery pockmarks itself
Fragrance remains to retain.
The purpose of the present invention is achieved by following technical proposals.
Unless otherwise indicated, percentage employed in the present invention is mass percent.
First aspect present invention discloses a kind of soft quasi- carbonization ferment particulate of fiery pockmarks, which is using fiery pockmarks as raw material
It carries out heating soft quasi- carbonization treatment, then passes through bacillus subtilis Van3 (bacterial strain preservation information.Bacterial strain deposit number: CGMCC
No.4091；Preservation date: on August 16th, 2010；Depositary institution: China Committee for Culture Collection of Microorganisms's commonly micro- life
Object center) it ferments to it, then it is 100~180 mesh powders that the intermediate product after fermentation, which is ground into partial size, uses adhesive
It is bonded into the particle that partial size is 20~60 mesh, the as soft quasi- carbonization ferment particulate of the fiery pockmarks.
Preferably, the condition of the soft quasi- carbonization treatment of heating are as follows: isolation air keeps 2~8h at 120~200 DEG C.
Preferably, described adhesive is the gelatin and CMS sodium solution that concentration is 5~12wt%, wherein gelatin with
Carboxymethyl starch mass ratio is 1:1~3.
Second aspect of the present invention discloses the preparation method of the soft quasi- carbonization ferment particulate of the fiery pockmarks, including walks as follows
It is rapid:
(1) dry fiery pockmarks are closed at 120~200 DEG C and 2~8h of holding carries out soft quasi- carbonization treatment；
(2) product after the soft quasi- carbonization treatment of step (1) is sprayed into bacillus subtilis Van3, then at 22 DEG C, relatively
24~72h of fermentation under conditions of humidity is 60%；
(3) raw material after step (2) fermentation is ground into partial size is 100~180 mesh powders, then uses adhesive bonding
It is the particle of 20~60 mesh at partial size, the as described fiery soft quasi- carbonization ferment particulate of pockmarks.
Preferably, bacillus subtilis Van3 described in step (2) is that test tube slant culture or seed culture obtain:
Test tube slant incubation step is as follows:
Using Luria-Bertani (LB) culture medium, formula are as follows: peptone 10g, sodium chloride 10g, yeast extract 5g,
Agar 20g, distilled water constant volume to 1000ml, 121 DEG C sterilize 25 minutes, are put into inclined-plane, are inoculated with Van35, and 28 DEG C of cultures for 24 hours, obtain
Test tube species are obtained, liquid spawn is transferred to the LB liquid medium of 1L by 5% (liquid spawn volume percentage, v/v) inoculum concentration
In, 37 DEG C, 150r/min shake culture 12h；Culture solution is centrifuged 10min with 3500r/min, with sterile water washing precipitating, is centrifuged,
Finally uniformly with the concussion of 20mL sterile water, 10 times, as the bacillus subtilis Van3 are diluted when use；
Steps are as follows for seed culture:
Using Luria-Bertani (LB) culture medium, formula are as follows: peptone 10g, sodium chloride 10g, yeast extract 5g,
Distilled water constant volume is to 1000ml；121 DEG C sterilize 25 minutes, are inoculated with Van35 test tube species, and 28 DEG C of cultures for 24 hours, obtain liquid spawn；
Liquid spawn is transferred in the LB liquid medium of 1L by 5% (liquid spawn volume percentage, v/v) inoculum concentration, 37 DEG C,
150r/min shake culture 12h；Culture solution is centrifuged 10min with 3500 r/min, with sterile water washing precipitating, centrifugation, finally uses
The concussion of 20mL sterile water uniformly, 10 times, as the bacillus subtilis Van3 is diluted when use.
Preferably, the preparation step of the fiery soft quasi- carbonization ferment particulate of pockmarks described in step (3) is as follows:
1. weigh 2~6 parts of gelatin, 3~6 parts of sodium carboxymethyl starch according to parts by weight, with distilled water be configured to concentration be 5~
The binder solution of 12wt%；
2. it is 100~180 mesh powders that the product after fermentation is ground into partial size in the case where moisture content is 8~12wt%；
3. it is 0.12~0.2Bar, gas flow temperature 50~60 that 2. powder that step obtains is fluidized air pressure in a fluidized bed
It is 5~15 minutes dry under the conditions of DEG C；
4. in fluidisation 0.12~0.25Bar of air pressure, 60~80 DEG C of gas flow temperature, 0.08~0.15Bar of atomisation pressure, hydrojet
Under conditions of rate 8~12g/ minutes, applying step using top spray mode, 1. 3. prepared binder solution is obtained to step
Drying after on powder, obtain the particle that moisture content is 10~12wt%, grain diameter is 20~60 mesh, the as described fire
The soft quasi- carbonization ferment particulate of pockmarks；Wherein described adhesive solution usage is 25%~50% of powder weight after drying；
Preferably, 3. the fluidized bed is Midi Glatt fluidized bed to step.
Third aspect present invention discloses the soft quasi- carbonization ferment particulate of the fiery pockmarks for the purposes in cigarette filter tip.
Preferably, the soft quasi- carbonization ferment particulate of the fiery pockmarks of 1~3mg is added in every millimeter of standard cigarettes filter stick.
Beneficial effects of the present invention:
1, fiery pockmarks of the invention are after soft quasi- carbonization treatment, and cell dehydration and is formed in a short time, increase in
Portion gap, the gap can harmful substance in selective absorption cigarette smoke, significantly improve the filter efficiency of its cigarette smoke.Together
Plant carbide temperature is not achieved in the soft quasi- carbonization technique temperature of Shi Jiare, which does not destroy the aromatic property of itself, fiery pockmarks
The special burnt odor taste of itself remains to retain.
2, through soft quasi- carbonization treatment again after microbial fermentation, producing has and cigarette flavor phase fiery pockmarks of the invention
The special aroma of coordination enriches cigarette cigarette perfume, while also having the effect of being obviously improved sweet sense.
3, preparation method of the invention is for the first time handled fiery pockmarks using soft quasi- carbonization and bacillus subtilis Van3.
It does not need to be adjusted plant granule production technology in specific production process, only by being modified just the simple of raw material
It being able to achieve and improves flavouring and tar reduction double effects, simple production process is easy to operate, and raw material is easy to get, and it is low in cost, it is easy to implement
Industrialized production has good application value.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is described in further detail.It should be appreciated that described herein, specific examples are only used to explain the present invention, is not to this hair
The restriction of bright technical solution.
Embodiment 1
1, the soft quasi- carbonization of fiery pockmarks
Fiery pockmarks are put into retort, furnace temperature is gradually heated to about 80 DEG C, keeps 40min, after fiery pockmarks are dry,
It is brought rapidly up to 120 DEG C, and keeps 3h, close heat source, it is spare to take out the soft quasi- carbonized samples of fiery pockmarks.It is brown that general raw material becomes ash
Color, weight soft quasi- carbonization completion when being the 50-80% before soft quasi- carbonization.
2, the culture of bacillus subtilis Van3 (B.subtilisVan3)
A. test tube slant culture
Using Luria-Bertani (LB) culture medium, formula are as follows: peptone 10g, sodium chloride 10g, yeast extract 5g,
Agar 20g, distilled water constant volume to 1000ml.121 DEG C sterilize 25 minutes, are put into inclined-plane.It is inoculated with Van35,28 DEG C of cultures for 24 hours, obtain
Test tube species are obtained, liquid spawn is transferred to the LB liquid medium of 1L by 5% (liquid spawn volume percentage, v/v) inoculum concentration
In, 37 DEG C, 150r/min shake culture 12h；Culture solution is centrifuged 10min with 3500r/min, with sterile water washing precipitating, is centrifuged,
Finally uniformly with the concussion of 20mL sterile water, 10 times, as the bacillus subtilis Van3 are diluted when use.
3, fiery pockmarks rub the fermentation of quasi- carbonization sample
Fiery pockmarks product after weighing the soft quasi- carbonization of 1000g, equilibrium water conten to 13wt% spray withered grass bud obtained above
Spore bacillus Van3 is 200mL, is then placed in 22 DEG C, the climatic chamber fermentation 48h of relative humidity 60%.
4, the preparation of the soft quasi- carbonization ferment particulate material of fiery pockmarks
Take above-mentioned tunning, detect its moisture content be 10wt% when, be further ground into partial size be 120 purposes
Powder；Then powder is placed in Midi Glatt fluidized bed and is granulated, fluidized bed fluidize air pressure be 0.2Bar, gas flow temperature 60
Dry 5min under the conditions of DEG C；Then 2 parts of gelatin, 8 parts of sodium carboxymethyl starch are weighed by weight, are configured to concentration with distilled water
For the binder solution of 10wt%；Fluidisation air pressure be 0.25Bar, gas flow temperature is 60 DEG C, atomisation pressure 0.15Bar, spray
Liquid speed rate is to apply 300g binder solution using top spray mode under conditions of 12g/ minutes；After adhesive has applied, fluidizing
It is 5 minutes dry under the conditions of air pressure is 0.2Bar, gas flow temperature is 60 DEG C, it is thick for 12wt%, surface that moisture content is prepared
Rough, partial size is the soft quasi- carbonization ferment particulate of fire pockmarks described in 40 purposes.
Embodiment 2
1, the soft quasi- carbonization of fiery pockmarks
Fiery pockmarks are put into retort, furnace temperature is gradually heated to 80 DEG C, keeps 30min, it is fast after fiery pockmarks are dry
Speed is warming up to 140 DEG C, and keeps 2.5h, closes heat source, and it is spare to take out the soft quasi- carbonized samples of fiery pockmarks.
2, the culture of bacillus subtilis Van3
B. seed culture
Using Luria-Bertani (LB) culture medium, formula are as follows: peptone 10g, sodium chloride 10g, yeast extract 5g,
Distilled water constant volume is to 1000ml.121 DEG C sterilize 25 minutes.Van35 test tube species are inoculated with, 28 DEG C of cultures for 24 hours, obtain liquid spawn,
Liquid spawn is transferred in the LB liquid medium of 1L by 5% (liquid spawn volume percentage, v/v) inoculum concentration, 37 DEG C,
150r/min shake culture 12h；Culture solution is centrifuged 10min with 3500 r/min, with sterile water washing precipitating, centrifugation, finally uses
The concussion of 20mL sterile water uniformly, 10 times, as the bacillus subtilis Van3 is diluted when use.
3, fiery pockmarks rub the fermentation of quasi- carbonization sample
Fiery pockmarks product after weighing the soft quasi- carbonization of 500g, equilibrium water conten to 12wt% spray withered grass bud obtained above
Spore bacillus Van3 is 100mL, is then placed in 22 DEG C, 72 h of climatic chamber fermentation of relative humidity 60%.
4. the preparation of the fiery soft quasi- carbonization ferment particulate of pockmarks
Take above-mentioned tunning, detect its moisture content be 12wt% when, be further ground into partial size be 160 purposes
Powder；Then powder is placed in Midi Glatt fluidized bed and is granulated, fluidized bed fluidisation air pressure is 0.15Bar, gas flow temperature is
Dry 15min under the conditions of 60 DEG C；Then 4 parts of gelatin, 4 parts of sodium carboxymethyl starch are weighed by weight, are configured to distilled water dense
Degree is the binder solution of 8wt%；Fluidisation air pressure be 0.2Bar, gas flow temperature is 60 DEG C, atomisation pressure 0.1Bar, hydrojet
Rate is to apply 200g binder solution using top spray mode under conditions of 10g/ minutes；After adhesive has applied, in fluidized gas
10 minutes dry under the conditions of pressure is 0.15Bar, gas flow temperature is 60 DEG C, it is 10%, rough surface, grain that moisture content, which is prepared,
Diameter is the soft quasi- carbonization ferment particulate of fire pockmarks described in 60 purposes.
Embodiment 3: the fiery soft quasi- carbonization ferment particulate of pockmarks is applied in cigarette filter rod
Embodiment 1 is obtained the fiery soft quasi- carbonization ferment particulate of pockmarks to be applied in cigarette composite cigarette filter stick, often
Millimeter standard cigarettes filter stick adds 1.5mg granular materials, is prepared into experiment cigarette, is named as No. 1 cigarette；Not add above-mentioned
The cigarette of grain is control sample, is adjusted by national standard " tobacco of GB/T16447~1996 and tobacco product adjust the atmospheric environment with test "
Section cigarette sample moisture content be 12wt%, by smoke panel test panel of expert by " the cigarette sense organ of GB5606.4~1996 technical requirements " rule
Surely cigarette sensory evaluating smoking and harmful ingredients in flue gas detection are carried out.Smoking result is shown in Table 1；Specific sense organ marking is shown in Table 2 and table 3.It presses
Flue gas conventional detection is carried out to cigarette according to national standard GB 5606.5-2005, testing result is shown in Table 4.According to GB/T 21130-2007
" measurement of benzo [a] pyrene in cigarette smoke condensates ", the YC/T 253-2008 " measurement of hydrogen cyanide in cigarette mainstream flue gas
Continuous flow method ", YC/T 255-2008 " the measurement high performance liquid chromatography of phenolic compound in cigarette mainstream flue gas ", YC/T
254-2008 " the measurement high performance liquid chromatography of main carbonyl compounds in main stream cigarette smoke ", YC/T 377-2010 " volume
The measurement chromatography of ions of ammonia in cigarette main flume ", the measurement of carbon monoxide is non-in GB/T 23356-2009 components in cigarette smoke gas phase
Scatter infrared method ", " measurement of the peculiar N- nitrosamine of cigarette mainstream smoke total particulate matter tobacco is high by TCJC-ZY-IV-005-2012
Effect liquid phase chromatogram-tandem mass spectrum is combined method " regulation carry out 7 kinds of harmful components detections of flue gas, testing result is shown in Table 5.
Embodiment 4: it is applied in cigarette filter rod
Embodiment 2 is obtained the fiery soft quasi- carbonization ferment particulate of pockmarks to be applied in cigarette composite cigarette filter stick, often
Millimeter standard cigarettes filter stick adds 3mg granular materials, is prepared into experiment cigarette, is named as No. 2 cigarettes, not add above-mentioned particle
Cigarette be control sample, by national standard " tobacco of GB/T16447~1996 and tobacco product adjust the atmospheric environment with test " adjusting
Cigarette sample moisture content be 12wt%, by smoke panel test panel of expert by " the cigarette sense organ of GB5606.4~1996 technical requirements " regulation
Carry out cigarette sensory evaluating smoking and harmful ingredients in flue gas detection.Smoking result is shown in Table 1；Specific sense organ marking is shown in Table 2 and table 3.According to
National standard GB 5606.5-2005 carries out flue gas conventional detection to cigarette, and testing result is shown in Table 4.According to GB/T 21130-2007
" measurement of benzo [a] pyrene in cigarette smoke condensates ", the YC/T 253-2008 " measurement of hydrogen cyanide in cigarette mainstream flue gas
Continuous flow method ", YC/T 255-2008 " the measurement high performance liquid chromatography of phenolic compound in cigarette mainstream flue gas ", YC/T
254-2008 " the measurement high performance liquid chromatography of main carbonyl compounds in main stream cigarette smoke ", YC/T 377-2010 " volume
The measurement chromatography of ions of ammonia in cigarette main flume ", the measurement of carbon monoxide is non-in GB/T 23356-2009 components in cigarette smoke gas phase
Scatter infrared method ", " measurement of the peculiar N- nitrosamine of cigarette mainstream smoke total particulate matter tobacco is high by TCJC-ZY-IV-005-2012
Effect liquid phase chromatogram-tandem mass spectrum is combined method " regulation carry out 7 kinds of harmful components detections of flue gas, testing result is shown in Table 5.
1 cigarette sensory evaluating smoking's result of table
2 sensory evaluation of table marking table
3 suction evaluation table of table
The soft quasi- carbonization ferment particulate of the fiery pockmarks shown by the smoking result in table 1- table 3 is added in cigarette, system
Cigarette smoke panel test point higher than control sample 0.6 point or so.Compared with control sample, the comfort characteristic mean height 0.6 of cigarette
Point, smoke transportation mean height 0.5 divides, and sweet taste mean height 0.43 divides in taste style, tart flavour mean height 0.3.Therefore of the invention
The fiery soft quasi- carbonization ferment particulate of pockmarks, which is added in cigarette, can be obviously improved cigarette perfume, increase the sweet sense of flue gas, hence it is evident that prominent fresh and sweet
Fragrant style and features, meanwhile, the effect for reducing cigarette smoke irritation, improving user throat comfort can increase cigarette perfume
Richness and sophistication have preferable cigarette application effect, especially remain with the special burnt odor taste of fiery pockmarks itself.
4 flue gas conventional analysis result of table
5 harmful components testing result of table
As shown in Table 4, compared with control sample, the cigarette main flue of the soft quasi- carbonization ferment particulate of fiery pockmarks of the invention is added
Total granules, smoking nicotine and CO decrease in gas, and especially tar content is substantially reduced, and averagely have dropped
It is more than 0.73mg/ branch；Flue gas moisture obviously increases simultaneously, averagely increases 0.37mg/ branch or so.
As shown in Table 5, compared with control sample, 7 in the cigarette of the soft quasi- carbonization ferment particulate of fiery pockmarks of the invention are added
Kind harmful components have different degrees of reduction, wherein No. 1 cigarette CO reduces 8.65% ((12.72-11.62)/12.72), B
(a) P, which reduces 21.10%, NNK, reduces 3.92%, and crotonaldehyde, which reduces 20.53%, HCN, reduces 18.38%, ammonium ion
3.91% is reduced, phenol reduces 30.25%；No. 2 cigarette CO, which reduce 9.43%, B (a) P, reduces 22.32%, NNK
5.88% is reduced, crotonaldehyde, which reduces 21.99%, HCN, reduces 18.89%, and ammonium ion reduces 4.08%, and phenol reduces
30.51%.This may have gap structure abundant due to the soft quasi- carbonization ferment particulate of fiery pockmarks of the invention, can pass through
The physical absorption and retention on surface increase the filtration to flue gas aerosol.Compared with common vinegar fibre filter stick, fire of the invention
The soft quasi- carbonization ferment particulate of pockmarks can significantly reduce the content of phenol, crotonaldehyde, HCN and B (a) P.
Claims (9)
1. a kind of fire pockmarks soft quasi- carbonization ferment particulate, which is characterized in that the particle be heat using fiery pockmarks as raw material it is soft
Quasi- carbonization treatment, then passes through bacillus subtilis Van3 (bacterial strain deposit number: CGMCC No.4091；Preservation date: 2010
On August 16,；Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center) it ferments to it, then
By the intermediate product after fermentation be ground into partial size be 100~180 mesh powders, with adhesive bonding at partial size be 20~60 purposes
Particle, the as soft quasi- carbonization ferment particulate of the fiery pockmarks.
2. the soft quasi- carbonization ferment particulate of fire pockmarks according to claim 1, which is characterized in that at the soft quasi- carbonization of heating
The condition of reason are as follows: isolation air keeps 2~8h at 120~200 DEG C.
3. the soft quasi- carbonization ferment particulate of fire pockmarks according to claim 1, which is characterized in that described adhesive is that concentration is
The gelatin and CMS sodium solution of 5~12wt%, wherein gelatin and sodium carboxymethyl starch mass ratio are 1:1~3.
4. a kind of preparation method of the soft quasi- carbonization ferment particulate of fire pockmarks, which comprises the steps of:
(1) dry fiery pockmarks are closed at 120~200 DEG C and 2~8h of holding carries out soft quasi- carbonization treatment；
(2) product after the soft quasi- carbonization treatment of step (1) is sprayed into bacillus subtilis Van3, then in 22 DEG C, relative humidity
Ferment 24~72h under conditions of being 60%；
(3) product after step (2) fermentation is ground into partial size is 100~180 mesh powders, then uses adhesive bonding granulating
Diameter is the particle of 20~60 mesh, as the soft quasi- carbonization ferment particulate of the fiery pockmarks.
5. the preparation method according to claim 4, which is characterized in that bacillus subtilis Van3 described in step (2) is
Test tube slant culture or seed culture obtain:
Test tube slant incubation step is as follows:
Using Luria-Bertani (LB) culture medium, formula are as follows: peptone 10g, sodium chloride 10g, yeast extract 5g, agar
20g, distilled water constant volume to 1000ml, 121 DEG C sterilize 25 minutes, are put into inclined-plane, are inoculated with Van35, and 28 DEG C of cultures for 24 hours, are tried
Liquid spawn is transferred in the LB liquid medium of 1L, 37 by pipe kind by 5% (liquid spawn volume percentage, v/v) inoculum concentration
DEG C, 150r/min shake culture 12h；Culture solution is centrifuged 10min with 3500r/min, with sterile water washing precipitating, is centrifuged, finally
Uniformly with the concussion of 20mL sterile water, 10 times, as the bacillus subtilis Van3 are diluted when use；
Steps are as follows for seed culture:
Using Luria-Bertani (LB) culture medium, formula are as follows: peptone 10g, sodium chloride 10g, yeast extract 5g, distillation
Water constant volume is to 1000ml；121 DEG C sterilize 25 minutes, are inoculated with Van35 test tube species, and 28 DEG C of cultures for 24 hours, obtain liquid spawn；By liquid
Body strain is transferred in the LB liquid medium of 1L by 5% (liquid spawn volume percentage, v/v) inoculum concentration, and 37 DEG C, 150r/
Min shake culture 12h；Culture solution with 3500r/min be centrifuged 10min, with sterile water washing precipitating, centrifugation, finally with 20mL without
The concussion of bacterium water uniformly, 10 times, as the bacillus subtilis Van3 is diluted when use.
6. the preparation method according to claim 4, which is characterized in that the fiery soft quasi- carbonization fermentation of pockmarks described in step (3)
The preparation step of particle is as follows:
1. weigh 2~6 parts of gelatin, 3~6 parts of sodium carboxymethyl starch according to parts by weight, with distilled water be configured to concentration be 5~
The binder solution of 12wt%；
2. it is 100~180 mesh powders that the product after fermentation is ground into partial size in the case where moisture content is 8~12wt%；
3. it is 50~60 DEG C of 0.12~0.2Bar, gas flow temperature conditions that 2. powder that step obtains is fluidized air pressure in a fluidized bed
Lower drying 5~15 minutes；
4. in fluidisation 0.12~0.25Bar of air pressure, 60~80 DEG C of gas flow temperature, 0.08~0.15Bar of atomisation pressure, hydrojet rate
Under conditions of 8~12g/ minutes, using top spray mode apply step 1. prepared binder solution to step 3. obtain it is dry
After dry on powder, the particle that moisture content is 10~12wt%, grain diameter is 20~60 mesh is obtained, the as described fiery pockmarks are soft
Quasi- carbonization ferment particulate；Wherein described adhesive solution usage is 25%~50% of powder weight after drying.
7. preparation method according to claim 6, which is characterized in that 3. the fluidized bed is that Midi Glatt flows to step
Change bed.
8. according to the soft quasi- carbonization ferment particulate of fire pockmarks described in claims 1 for the purposes in cigarette filter tip.
9. purposes according to claim 8, which is characterized in that add the institute of 1~3mg in every millimeter of standard cigarettes filter stick
State the soft quasi- carbonization ferment particulate of fiery pockmarks.
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| CN201811218025.3A CN109363238A (en) | 2018-10-18 | 2018-10-18 | A kind of soft quasi- carbonization ferment particulate of fire pockmarks, preparation method and the usage |
| CN201910458407.1A CN110292203B (en) | 2018-10-18 | 2019-05-29 | Plant soft-simulated carbonized fermentation particles, and preparation method and application thereof |
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| CN201811218025.3A CN109363238A (en) | 2018-10-18 | 2018-10-18 | A kind of soft quasi- carbonization ferment particulate of fire pockmarks, preparation method and the usage |
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