CN109362563A - The rapid propagation method of the red Rett of Louisiana's iris - Google Patents

The rapid propagation method of the red Rett of Louisiana's iris Download PDF

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Publication number
CN109362563A
CN109362563A CN201811286232.2A CN201811286232A CN109362563A CN 109362563 A CN109362563 A CN 109362563A CN 201811286232 A CN201811286232 A CN 201811286232A CN 109362563 A CN109362563 A CN 109362563A
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culture medium
louisiana
iris
red
rett
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CN109362563B (en
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裘金玉
沈士华
蔡燕菁
王倩
骆樟泽
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HANGZHOU CHENHANG ENVIRONMENT ENGINEERING Co Ltd
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HANGZHOU CHENHANG ENVIRONMENT ENGINEERING Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The rapid propagation method of the red Rett of Louisiana's iris, belongs to technical field of plant propagation.It is the following steps are included: 1) Initial culture；2) Fiber differentiation；3) Multiplying culture；4) hardening is carried out after 8~10cm of height of seedling.The beneficial effects of the present invention are: the present invention realizes the efficient quick breeding of the red Rett of Louisiana's iris by primary, induction, Multiplying culture.By special induction, the use of proliferated culture medium, root induction while capable of accomplishing to be quickly obtained bud proliferation during induction, Multiplying culture shortens and expands numerous time, reduces costs the present invention.

Description

The rapid propagation method of the red Rett of Louisiana's iris
Technical field
The invention belongs to technical field of plant propagation, and in particular to the quick breeding side of the red Rett of Louisiana's iris Method.
Background technique
Louisiana iris (Louisiana Iris) " red Rett " originates in United States Louisiana, Florida The marshland in the Gulfs such as state area and Mississippi Delta basin, is a natural hybrid, belongs to Iridaceae The raw herbaceous plant of water-wet, since its winter is evergreen, pattern is abundant, and flower is gorgeous, has high ornamental value, while root system Prosperity can adsorb the rich nutritive materials such as nitrogen phosphorus and heavy metal element in water, have and purify water and as bank protection plant material Material was made scape in park in recent years, was widely applied in river regulation project.
However Louisiana iris (Louisiana Iris) " red Rett ", seed nature germination rate is low, breeding coefficient Low, supply falls short of demand for the Louisiana iris (Louisiana Iris) " red Rett " for causing in the market.
Summary of the invention
In view of the problems of the existing technology, it is an object of the invention to design, to provide a kind of Louisiana's iris red auspicious The technical solution of special rapid propagation method.
The rapid propagation method of the red Rett of Louisiana's iris, it is characterised in that the following steps are included:
1) the red Rett full maturity seed of Louisiana's iris is taken, taking mature embryo is explant, is taken out after cleaned and disinfection Embryo, and be inoculated into bacterium of having gone out pH value be 5.6 MS solid medium on, temperature be 25 DEG C, photoperiod 14/10h, light It is cultivated in the culturing room of strong 2000lux；
2) it after Initial culture 20 days, is transferred in induced medium, induced medium is basic culture medium with MS culture medium, is added dense Degree is the 6-BA of 0.5~1.5mg/L, and concentration is the IAA of 1.5~2.5mg/L, and pH value 5.6 is 25 DEG C in temperature, the photoperiod 14/ 10h is cultivated in the culturing room of light intensity 2000lux；
3) it after Fiber differentiation 15 days, is transferred in proliferated culture medium, proliferated culture medium is basic culture medium with MS culture medium, is added dense Degree is the IBA of 0.3~2mg/L, and concentration is the NAA of 0.5~1.5mg/L, and pH value 5.6 is 25 DEG C, photoperiod 16/8h in temperature, It is cultivated in the culturing room of light intensity 2500lux；
4) hardening is carried out after 8~10cm of height of seedling.
The rapid propagation method of the red Rett of Louisiana's iris, it is characterised in that induction in the step 2) Culture medium is basic culture medium with MS culture medium, and the 6-BA that addition concentration is 1mg/L, concentration is the IAA of 2mg/L.
The rapid propagation method of the red Rett of Louisiana's iris, it is characterised in that proliferation in the step 3) Culture medium is basic culture medium with MS culture medium, and the IBA that addition concentration is 1.5mg/L, concentration is the NAA of 1mg/L.
Using the beneficial effects of the present invention are: the present invention realizes Louisiana by primary, induction, Multiplying culture The efficient quick breeding of the red Rett of iris.The present invention is by special induction, the use of proliferated culture medium, in induction, proliferation training Root induction while capable of accomplishing to be quickly obtained bud proliferation during supporting, shortens and expands numerous time, reduce costs.
Specific embodiment
Further illustrate the present invention with reference to embodiments.
Embodiment 1
1) the red Rett full maturity seed of Louisiana's iris is taken, taking mature embryo is explant, is taken out after cleaned and disinfection Embryo, and be inoculated into bacterium of having gone out pH value be 5.6 MS solid medium on, temperature be 25 DEG C, photoperiod 14/10h, light It is cultivated in the culturing room of strong 2000lux；
2) it after Initial culture 20 days, is transferred in induced medium, induced medium is basic culture medium with MS culture medium, is added dense Degree is the 6-BA of 1mg/L, and concentration is the IAA of 2mg/L, and pH value 5.6 is 25 DEG C, photoperiod 14/10h in temperature, light intensity It is cultivated in the culturing room of 2000lux；
3) it after Fiber differentiation 15 days, is transferred in proliferated culture medium, proliferated culture medium is basic culture medium with MS culture medium, is added dense Degree is the IBA of 1.5mg/L, and concentration is the NAA of 1mg/L, and pH value 5.6 is 25 DEG C, photoperiod 16/8h in temperature, light intensity It is cultivated in the culturing room of 2500lux；
4) hardening is carried out after 8~10cm of height of seedling.
The red Rett of Louisiana's iris is most cultivated 3 days fastly and can be germinateed in the embodiment 1, and average germination percentage reaches in 35 days To 92.6%, germinating time is substantially reduced, substantially increases germination percentage, radicle length can be to 3.5~4.5cm, significantly in 45 days Planting percent is improved into, planting percent has reached 98.5%.
Embodiment 2
1) the red Rett full maturity seed of Louisiana's iris is taken, taking mature embryo is explant, is taken out after cleaned and disinfection Embryo, and be inoculated into bacterium of having gone out pH value be 5.6 MS solid medium on, temperature be 25 DEG C, photoperiod 14/10h, light It is cultivated in the culturing room of strong 2000lux；
2) it after Initial culture 20 days, is transferred in induced medium, induced medium is basic culture medium with MS culture medium, is added dense Degree is the 6-BA of 1.5mg/L, and concentration is the IAA of 2.5mg/L, and pH value 5.6 is 25 DEG C, photoperiod 14/10h in temperature, light intensity It is cultivated in the culturing room of 2000lux；
3) it after Fiber differentiation 15 days, is transferred in proliferated culture medium, proliferated culture medium is basic culture medium with MS culture medium, is added dense Degree is the IBA of 2mg/L, and concentration is the NAA of 1.5mg/L, and pH value 5.6 is 25 DEG C, photoperiod 16/8h in temperature, light intensity It is cultivated in the culturing room of 2500lux；
4) hardening is carried out after 8~10cm of height of seedling.
The red Rett of Louisiana's iris is most cultivated 3 days fastly and can be germinateed in the embodiment 2, and average germination percentage reaches in 35 days To 90.8%, germinating time is substantially reduced, substantially increases germination percentage, radicle length can be to 3.3~4.2cm, significantly in 45 days Planting percent is improved, planting percent has reached 97.4%.
Embodiment 3
1) the red Rett full maturity seed of Louisiana's iris is taken, taking mature embryo is explant, is taken out after cleaned and disinfection Embryo, and be inoculated into bacterium of having gone out pH value be 5.6 MS solid medium on, temperature be 25 DEG C, photoperiod 14/10h, light It is cultivated in the culturing room of strong 2000lux；
2) it after Initial culture 20 days, is transferred in induced medium, induced medium is basic culture medium with MS culture medium, is added dense Degree is the 6-BA of 0.5mg/L, and concentration is the IAA of 1.5mg/L, and pH value 5.6 is 25 DEG C, photoperiod 14/10h in temperature, light intensity It is cultivated in the culturing room of 2000lux；
3) it after Fiber differentiation 15 days, is transferred in proliferated culture medium, proliferated culture medium is basic culture medium with MS culture medium, is added dense Degree is the IBA of 0.3mg/L, and concentration is the NAA of 0.5mg/L, and pH value 5.6 is 25 DEG C, photoperiod 16/8h in temperature, light intensity It is cultivated in the culturing room of 2500lux；
4) hardening is carried out after 8~10cm of height of seedling.
The red Rett of Louisiana's iris is most cultivated 3 days fastly and can be germinateed in the embodiment 3, and average germination percentage reaches in 35 days To 91.4%, germinating time is substantially reduced, substantially increases germination percentage, radicle length can be to 3.4~4.1cm, significantly in 45 days Planting percent is improved, planting percent has reached 97.8%.

Claims (3)

1. the rapid propagation method of the red Rett of Louisiana's iris, it is characterised in that the following steps are included:
1) the red Rett full maturity seed of Louisiana's iris is taken, taking mature embryo is explant, is taken out after cleaned and disinfection Embryo, and be inoculated into bacterium of having gone out pH value be 5.6 MS solid medium on, temperature be 25 DEG C, photoperiod 14/10h, light It is cultivated in the culturing room of strong 2000lux；
2) it after Initial culture 20 days, is transferred in induced medium, induced medium is basic culture medium with MS culture medium, is added dense Degree is the 6-BA of 0.5~1.5mg/L, and concentration is the IAA of 1.5~2.5mg/L, and pH value 5.6 is 25 DEG C in temperature, the photoperiod 14/ 10h is cultivated in the culturing room of light intensity 2000lux；
3) it after Fiber differentiation 15 days, is transferred in proliferated culture medium, proliferated culture medium is basic culture medium with MS culture medium, is added dense Degree is the IBA of 0.3~2mg/L, and concentration is the NAA of 0.5~1.5mg/L, and pH value 5.6 is 25 DEG C, photoperiod 16/8h in temperature, It is cultivated in the culturing room of light intensity 2500lux；
4) hardening is carried out after 8~10cm of height of seedling.
2. the rapid propagation method of the red Rett of Louisiana's iris as described in claim 1, it is characterised in that the step It is rapid 2) in induced medium with MS culture medium be basic culture medium, the 6-BA that addition concentration is 1mg/L, concentration is 2mg/L's IAA。
3. the rapid propagation method of the red Rett of Louisiana's iris as described in claim 1, it is characterised in that the step It is rapid 3) in proliferated culture medium with MS culture medium be basic culture medium, the IBA that addition concentration is 1.5mg/L, concentration is 1mg/L's NAA。
CN201811286232.2A 2018-10-31 2018-10-31 Rapid propagation method of Louisiana irisorite Active CN109362563B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110476817A (en) * 2019-09-29 2019-11-22 上海市农业科学院 A kind of iris radioinduction dose screening method
CN114027188A (en) * 2021-11-02 2022-02-11 江苏省中国科学院植物研究所 Culture medium for obtaining iris interspecific hybridization progeny by utilizing immature embryos and application of culture medium
CN114403000A (en) * 2021-12-31 2022-04-29 湖北懿昌能兴生态农业科技有限公司 Louisiana iris tissue culture propagation method with high plant regeneration rate
CN116649218A (en) * 2023-06-28 2023-08-29 江苏省中国科学院植物研究所 Efficient breeding method of Lissanamia iris

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EP0438354A1 (en) * 1990-01-17 1991-07-24 Shiseido Company Limited Protoplasts of a plant of the genus Iris and methods of isolating and culturing same
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110476817A (en) * 2019-09-29 2019-11-22 上海市农业科学院 A kind of iris radioinduction dose screening method
CN114027188A (en) * 2021-11-02 2022-02-11 江苏省中国科学院植物研究所 Culture medium for obtaining iris interspecific hybridization progeny by utilizing immature embryos and application of culture medium
CN114403000A (en) * 2021-12-31 2022-04-29 湖北懿昌能兴生态农业科技有限公司 Louisiana iris tissue culture propagation method with high plant regeneration rate
CN116649218A (en) * 2023-06-28 2023-08-29 江苏省中国科学院植物研究所 Efficient breeding method of Lissanamia iris

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