CN109358148A - 血液中维生素d的检验方法 - Google Patents

血液中维生素d的检验方法 Download PDF

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Publication number
CN109358148A
CN109358148A CN201811097358.5A CN201811097358A CN109358148A CN 109358148 A CN109358148 A CN 109358148A CN 201811097358 A CN201811097358 A CN 201811097358A CN 109358148 A CN109358148 A CN 109358148A
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column
sample
vitamin
detected
mobile phase
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李江西
卢勇
陈秀玉
刘晓磊
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Hangzhou Hehe Medical Laboratory Co Ltd
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Hangzhou Hehe Medical Laboratory Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8822—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving blood

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  • Urology & Nephrology (AREA)
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Abstract

本发明公开了血液中维生素D的检验方法，包括以下步骤：步骤1：抽取测试者的血清样品，待检测；步骤2：在血清样品中加入维生素D释放剂，进行蛋白沉淀、离心，得到测试样品；步骤3：对测试样品采用二维液相色谱串联四级杆质谱联用仪进行富集、分离和检测：步骤4：用流动相1洗脱富集柱中的样品，再将富集柱与分析柱相连通，用流动相2将待测样品从富集柱洗脱至分析柱，在断开富集柱与分析柱，用流动相2将待测物从分析柱洗脱、分离至四级杆质谱进行检测，本发明测试过程精简，测试过程抗基质干扰能力强，测试灵敏度高、精确度高。

Description

血液中维生素D的检验方法
技术领域
本发明涉及一种检验方法，特别涉及一种血液中维生素D的检验方法。
背景技术
维生素D（vitamin D ）为固醇类衍生物，具抗佝偻病作用，又称抗佝偻病维生素。目前认为维生素D也是一种类固醇激素，维生素D家族成员中最重要的成员是VD2（麦角钙化醇）和VD3（胆钙化醇)。维生素D是一种脂溶性维生素，有五种化合物，对健康关系较密切的是维生素D2和维生素D3。它们有以下三点特性：它存在于部分天然食物中；人体皮下储存有从胆固醇生成的7-脱氢胆固醇，受紫外线的照射后，可转变为维生素D3。维生素D在血液形成中有以下益处：维生素D调节控制钙、磷代谢及骨骼的生长发育，使血钙、血磷保持正常水平。
发明内容
本发明的目的在于提供一种血液中维生素D的检验方法。
本发明采用的技术方案是：
血液中维生素D的检验方法，其特征在于：包括以下步骤：
步骤1：抽取测试者的血清样品，待检测；
步骤2：在血清样品中加入维生素D释放剂，进行蛋白沉淀、离心，得到测试样品；
步骤3：对测试样品采用二维液相色谱串联四级杆质谱联用仪进行富集、分离和检测：
步骤4：用流动相1洗脱富集柱中的样品，再将富集柱与分析柱相连通，用流动相2将待测样品从富集柱洗脱至分析柱，在断开富集柱与分析柱，用流动相2将待测物从分析柱洗脱、分离至四级杆质谱进行检测。
本发明的优点：测试过程精简，测试过程抗基质干扰能力强，测试灵敏度高、精确度高。
具体实施方式
血液中维生素D的检验方法，包括以下步骤：
步骤1：抽取测试者的血清样品，待检测；
步骤2：在血清样品中加入维生素D释放剂，进行蛋白沉淀、离心，得到测试样品；
步骤3：对测试样品采用二维液相色谱串联四级杆质谱联用仪进行富集、分离和检测：
步骤4：用流动相1洗脱富集柱中的样品，再将富集柱与分析柱相连通，用流动相2将待测样品从富集柱洗脱至分析柱，在断开富集柱与分析柱，用流动相2将待测物从分析柱洗脱、分离至四级杆质谱进行检测。
本发明测试过程精简，测试过程抗基质干扰能力强，测试灵敏度高、精确度高。

Claims (1)

1.血液中维生素D的检验方法，其特征在于：包括以下步骤：
步骤1：抽取测试者的血清样品，待检测；
步骤2：在血清样品中加入维生素D释放剂，进行蛋白沉淀、离心，得到测试样品；
步骤3：对测试样品采用二维液相色谱串联四级杆质谱联用仪进行富集、分离和检测：
步骤4：用流动相1洗脱富集柱中的样品，再将富集柱与分析柱相连通，用流动相2将待测样品从富集柱洗脱至分析柱，在断开富集柱与分析柱，用流动相2将待测物从分析柱洗脱、分离至四级杆质谱进行检测。
CN201811097358.5A 2018-09-20 2018-09-20 血液中维生素d的检验方法 Withdrawn CN109358148A (zh)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101467048A (zh) * 2006-06-06 2009-06-24 霍夫曼-拉罗奇有限公司 改良的维生素d测量方法
CN103308621A (zh) * 2013-06-18 2013-09-18 广州金域医学检验中心有限公司 高通量液相色谱法串联质谱法检测25羟基维生素d的方法
CN106526026A (zh) * 2016-11-10 2017-03-22 广州博厚医疗技术有限公司 血清中25‑羟基维生素d的检测方法
CN108267602A (zh) * 2010-12-28 2018-07-10 未来诊断有限公司 对于维生素d的释放剂
CN108490096A (zh) * 2018-04-16 2018-09-04 南方医科大学 人血清中25羟基维生素d的检测方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101467048A (zh) * 2006-06-06 2009-06-24 霍夫曼-拉罗奇有限公司 改良的维生素d测量方法
CN108267602A (zh) * 2010-12-28 2018-07-10 未来诊断有限公司 对于维生素d的释放剂
CN103308621A (zh) * 2013-06-18 2013-09-18 广州金域医学检验中心有限公司 高通量液相色谱法串联质谱法检测25羟基维生素d的方法
CN106526026A (zh) * 2016-11-10 2017-03-22 广州博厚医疗技术有限公司 血清中25‑羟基维生素d的检测方法
CN108490096A (zh) * 2018-04-16 2018-09-04 南方医科大学 人血清中25羟基维生素d的检测方法

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Inventor after: Li Jiangxi

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Inventor before: Lu Yong

Inventor before: Chen Xiuyu

Inventor before: Liu Xiaolei

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Application publication date: 20190219