CN109355162A - 一种实时荧光定量pcr加样96孔板暂存盒 - Google Patents

一种实时荧光定量pcr加样96孔板暂存盒 Download PDF

Info

Publication number
CN109355162A
CN109355162A CN201810408629.8A CN201810408629A CN109355162A CN 109355162 A CN109355162 A CN 109355162A CN 201810408629 A CN201810408629 A CN 201810408629A CN 109355162 A CN109355162 A CN 109355162A
Authority
CN
China
Prior art keywords
sample
real
quantitative pcr
temporary storage
time fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201810408629.8A
Other languages
English (en)
Inventor
屈艳琳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
JINING MEDICAL COLLEGE
Original Assignee
JINING MEDICAL COLLEGE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by JINING MEDICAL COLLEGE filed Critical JINING MEDICAL COLLEGE
Priority to CN201810408629.8A priority Critical patent/CN109355162A/zh
Publication of CN109355162A publication Critical patent/CN109355162A/zh
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

本发明是实时荧光定量PCR实验中96孔板(或八连管)加样后的一种暂存盒，属于分子生物学研究领域的一种实验工具。实时荧光定量大批量检测时，许多实验者习惯于先把各个孔所需的公用试剂先混合再分装，不仅可以减少工作量，还可以使混合更充分，误差更小，一次加完很多待测样本。但一台实时荧光定量PCR仪一般两个小时最多检测96个孔的数值，就经常会遇到样本需要等待机器检测的情况。如果把96孔板(或八连管)直接放入4℃冰箱，样本中已经混合有荧光基团，其他人反复开冰箱门，冰箱灯亮会影响实验数据的准确性。本发明六面棕色遮光处理，可最大程度减小冰箱里灯光对实验数据的影响，同时发明的底部可以放入冰块，使样本可以短途运输。

Description

一种实时荧光定量PCR加样96孔板暂存盒
技术领域
本发明是生物分子水平研究领域，实时荧光定量技术检测样本时，加样样本的暂存盒。
背景技术
聚合酶链反应(PCR)是生命遗传物质DNA的体外扩增，而实时荧光定量技术，在传统的PCR反应中加入一种荧光基团，利用荧光信号累计来监测整个PCR进程，通过数据分析，实现样本的定量检测，使 PCR技术从定性到定量有了质的飞跃。实时荧光定量PCR检测是最近兴起的一种分子水平的检测方法，以其灵敏度高、特异性强、重复性好、速度快、安全可靠、自动化程度高等特点迅速成为分子生物学研究的重要工具。
实时荧光定量PCR检测前需要先加样，即每个检测孔均需加入上下游引物、水、模板、荧光基团等。大批量做实时荧光定量PCR检测时(比如检测很多样本的某种病原体、筛选分子水平的诊断生物标志物等)，需要加很多样孔，为了加样的方便，很多实验者先把各个孔共用的试剂先混合，再分装，这样不仅极大减小了工作量，而且反应液混合更均匀。
但是一台实时荧光定量PCR机一次(大约两个小时)最多检测96个孔的数值，经常遇到加好的样本等待检测的情况。此时样本中已经混合有荧光基团，为了保证实验数据的准确性，设计了该发明。
发明内容
该发明是一个六面均为棕色的PC材料的盒子，外部分盖和体，内部由两隔板分三层，第一层隔板为可以放入96孔板的塑料板，第二层隔板稍低于96孔(八连管)的最底部，第二层隔板下可以加入水或冰袋。放入4℃冰箱，六面棕色设计，可防止其他人反复开冰箱门，冰箱灯亮造成对样本的影响，该盒子第二层隔板下放入冰袋，可以实现样本的较短途运输。
附图说明
图1.该发明整体俯视图；图2.第一层隔板俯视图；图3.该发明侧面图，图3中1-本发明的盖子；2- 第一层隔板(96孔板)；3-第二层隔板(无孔的PC板)；4-冰块放置区
具体实施方式
本发明在具体使用时，使用者将加好的样本(96孔板或者八连管)放在第一层隔板上，盖好盖子，放入4℃冰箱即可。
如果样本需要短距离转移，在加冰区加入冰块即可。

Claims (4)

1.该发明六面棕色遮光处理。
2.该发明外部整体分两部分，盒盖和盒体，盒盖略大于盒体。
3.该发明内部分三层，第一层隔板为96孔板，第二层为无孔的PC板。
4.该发明第二层无孔PC板下有放置冰块空间。
CN201810408629.8A 2018-04-24 2018-04-24 一种实时荧光定量pcr加样96孔板暂存盒 Withdrawn CN109355162A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810408629.8A CN109355162A (zh) 2018-04-24 2018-04-24 一种实时荧光定量pcr加样96孔板暂存盒

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810408629.8A CN109355162A (zh) 2018-04-24 2018-04-24 一种实时荧光定量pcr加样96孔板暂存盒

Publications (1)

Publication Number Publication Date
CN109355162A true CN109355162A (zh) 2019-02-19

Family

ID=65349621

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810408629.8A Withdrawn CN109355162A (zh) 2018-04-24 2018-04-24 一种实时荧光定量pcr加样96孔板暂存盒

Country Status (1)

Country Link
CN (1) CN109355162A (zh)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130029333A1 (en) * 2011-07-29 2013-01-31 Ahjeong Son Magnetic Bead Quantum Dot Nanoparticle Assay
CN205188265U (zh) * 2015-12-01 2016-04-27 北京信诺佰世医学检验所有限公司 Qubit荧光定量管放置盒
CN106938205A (zh) * 2017-05-10 2017-07-11 贵州大学 一种多功能低温孔板装置
CN206666512U (zh) * 2017-04-18 2017-11-24 中国农业科学院兰州畜牧与兽药研究所 一种pcr实验操作实验盘
WO2017214948A1 (zh) * 2016-06-16 2017-12-21 毛侃琅 一种敲低人 miRNA-148a 表达的慢病毒载体的构建及其应用
CN208104446U (zh) * 2018-01-31 2018-11-16 天津双佛生物科技有限公司 一种遮光pcr加样器

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130029333A1 (en) * 2011-07-29 2013-01-31 Ahjeong Son Magnetic Bead Quantum Dot Nanoparticle Assay
CN205188265U (zh) * 2015-12-01 2016-04-27 北京信诺佰世医学检验所有限公司 Qubit荧光定量管放置盒
WO2017214948A1 (zh) * 2016-06-16 2017-12-21 毛侃琅 一种敲低人 miRNA-148a 表达的慢病毒载体的构建及其应用
CN206666512U (zh) * 2017-04-18 2017-11-24 中国农业科学院兰州畜牧与兽药研究所 一种pcr实验操作实验盘
CN106938205A (zh) * 2017-05-10 2017-07-11 贵州大学 一种多功能低温孔板装置
CN208104446U (zh) * 2018-01-31 2018-11-16 天津双佛生物科技有限公司 一种遮光pcr加样器

Similar Documents

Publication Publication Date Title
Bæk et al. The impact of various preanalytical treatments on the phenotype of small extracellular vesicles in blood analyzed by protein microarray
Mathur et al. Microfluidics as an enabling technology for personalized cancer therapy
Bagwell et al. Multi‐site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system
Krutzik et al. Phospho flow cytometry methods for the analysis of kinase signaling in cell lines and primary human blood samples
NZ335863A (en) Apparatus and method for conducting assays such as blood screening
US20200408752A1 (en) Fluidic system for performing assays
RU2014119996A (ru) Обнаружение, выделение и анализ редких клеток в биологических жидкостях
CN107406510A (zh) 前列腺抗原标准品及其用途
WO2017118128A1 (zh) 多种血清标志物综合检测设备
US20220397528A1 (en) Systems and methods for rapid, sensitive multiplex immunoassays
Maecker et al. New technologies for autoimmune disease monitoring
EP3906411A1 (en) Assays with reduced interference (iii)
Saccenti et al. Systems biology and biomarkers in necrotizing soft tissue infections
CN107179415A (zh) 生物样品用分析仪的质量对照物
US20210268494A1 (en) Methods, devices, and related aspects for collecting and storing samples
KR100724316B1 (ko) 마이크로 플루이딕 칩 및 그 용도
CN109355162A (zh) 一种实时荧光定量pcr加样96孔板暂存盒
US20130122606A1 (en) Method and device for chemical and/or biological analysis
Chen et al. C1q assay for the detection of complement fixing antibody to HLA antigens
CN111500407A (zh) 一种基于三层微结构的ctc捕捉检测芯片
JP2007292718A (ja) 多項目試験紙および検体中の被検物質の検出方法
US10782288B2 (en) Multi-unit for conducting biochemical test and immunological test and testing method thereof
CN205650221U (zh) 酶联免疫快速检测的微流控芯片
US8603415B2 (en) Microchip
Landry et al. Studying cellular signal transduction with OMIC technologies

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication

Application publication date: 20190219

WW01 Invention patent application withdrawn after publication