CN109351373A - A kind of unidirectional built-in filter membrane micro-fluidic chip intercepting impurity - Google Patents
A kind of unidirectional built-in filter membrane micro-fluidic chip intercepting impurity Download PDFInfo
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- CN109351373A CN109351373A CN201811430893.8A CN201811430893A CN109351373A CN 109351373 A CN109351373 A CN 109351373A CN 201811430893 A CN201811430893 A CN 201811430893A CN 109351373 A CN109351373 A CN 109351373A
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- sample
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- filter membrane
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
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Abstract
The present invention discloses a kind of unidirectional built-in filter membrane micro-fluidic chip that can intercept impurity.It is made of substrate and filter membrane bonding, the " Kan " shape chamber of both ends open is provided in substrate, which includes sample inlet, sample detection unit, sample export, and the sample inlet is sequentially connected logical with sample detection unit and sample export；The sample inlet is located at a wherein arm for substrate " Kan " shape chamber, specifically includes sample filtering room, filtrate collection chamber, filter membrane composition；Sample export is located at another arm of substrate " Kan " shape chamber, specifically includes sample export room, sample export chamber inlet.This product structure is simple, and cost is extremely low.
Description
Technical field
The invention belongs to micro-fluidic chips, and the micro-fluidic core of 20 μm or more impurity can be intercepted more particularly to a kind of sample inlet
Piece.
Background technique
Micro-fluidic chip in the market includes sample inlet, sample detection unit and sample export, this micro-fluidic chip
Sample inlet cannot intercept the impurity in measuring samples, due to that can contain impurity in measuring samples, special interception need to be set
Unit intercepts the impurity in measuring samples, thus this microfluidic chip structure is complicated.
Summary of the invention
The object of the present invention is to provide a kind of structure, simple and sample inlet can intercept diameter in measuring samples and be greater than 20 μm
The micro-fluidic chip of impurity.
To achieve the goals above, the technical scheme is that
A kind of micro-fluidic chip is made of substrate and filter membrane bonding, the " Kan " shape chamber of both ends open is provided in substrate, should
Chamber includes sample inlet, sample detection unit, sample export, the sample inlet and sample detection unit and sample export
It is sequentially connected logical；The sample detection unit is existing mature detection module.
The sample inlet is located at a wherein arm for substrate " Kan " shape chamber, specifically includes sample filtering room, filtrate is collected
Room, filter membrane；Sample filtering chamber inlet is located at sample filtering ceiling portion, and the bottom of sample filtering room and the entrance of filtrate collection chamber connect
Logical, the bottom of sample filtering room and the entrance opening dimension of filtrate collection chamber are consistent, and are separated between the two by filter membrane；Filtrate collection chamber
Outlet the side wall of substrate " Kan " shape chamber arm, and the entrance phase of the outlet of filtrate collection chamber and sample detection unit are set
Connection；
The sample filtering chamber room of above-mentioned micro-fluidic chip is cuboid, and bottom surface is square；2000 μm≤square bottom
Face side length≤4000 μm, 50 μm≤cuboid chamber height≤80 μm.
The filtrate collection chamber of above-mentioned micro-fluidic chip is cuboid, and bottom surface is square；2000 μm≤square bottom surface side
It is≤4000 μm long, 50 μm≤cuboid chamber height≤80 μm.
The filter membrane for separating sample filtering room and filtrate collection chamber in above-mentioned micro-fluidic chip, aperture be 20 μm and with
Sample inlet chamber seamless adhesion.
The width of the filtrate collection chamber side wall filtrate collection chamber outlet of above-mentioned micro-fluidic chip is 594-606 μm, is highly
30-50μm。
The sample export of above-mentioned micro-fluidic chip is located at another arm of substrate " Kan " shape chamber, specifically includes sample export
Room, sample export chamber inlet；Sample export chamber inlet is located at the side wall of substrate " Kan " shape chamber arm, and with sample detection unit
Outlet be connected；
The sample export room of above-mentioned micro-fluidic chip is cuboid, and bottom surface is square, 2000 μm≤square bottom surface
Side length≤4000 μm, 100 μm≤cuboid chamber height≤160 μm.
The width of the sample export room sidewall sample outlet chamber inlet of above-mentioned micro-fluidic chip is 594-606 μm, is highly
30-50μm。
Benefit of the invention is that: 1) filter membrane that the sample filtering room bottom setting aperture of sample inlet be 20 μm can intercept
It is greater than 20 μm of impurity in sample to be tested, structure is simple, and cost is extremely low；2) filtrate collection chamber is located at sample filtering room bottom,
Impurity in sample greater than 20 μm is intercepted in sample filtering room and filtrate then flows into filtrate collection chamber, therefore this micro-fluidic
Chip sample entrance impurity in buffer belt test sample greater than 20 μm and can allow remaining substance to flow into filtrate collection chamber outlet.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of embodiment of micro-fluidic chip of the invention；
Fig. 2 is the top view of micro-fluidic chip of the invention；
Fig. 3 is the stereoscopic schematic diagram of micro-fluidic chip of the invention；
Fig. 4 is the top view of sample entrance port；
Fig. 5 is the side view of sample entrance port.
In figure: sample inlet 1, sample filtering room 1-1, filter membrane 1-2, filtrate collection chamber 1-3, sample filtering chamber inlet 1-4,
Filtrate collection chamber exports 1-5, sample detection unit 2, sample export 3, sample export chamber inlet 3-1, sample export room 3-2.
Specific embodiment
Below in conjunction with attached drawing and the embodiment provided, the present invention is further illustrated.
As shown in Figs. 1-5, a kind of micro-fluidic chip for intercepting diameter and being greater than 20 μm of impurity, is bonded structure by substrate and filter membrane
At, including sample inlet 1 and sample export 3, the sample inlet 1 be connected with sample export 3；
The sample inlet 1 includes sample filtering room 1-1, filter membrane 1-2, filtrate collection chamber 1-3, sample filtering chamber inlet 1-
4 and filtrate collection chamber outlet 1-5.Sample filtering chamber inlet 1-4 is located at the top of the 1-1 of sample filtering room, and filtrate collection chamber exports 1-5
Positioned at filtrate collection chamber 1-3 side wall.
The sample filtering room and filtrate collection chamber are located in the same chamber of sample inlet, and centre is separated by filter membrane.
The size of above-mentioned sample filtering room 1-1 are as follows: the bottom surface side of the chamber for the cuboid that 2000 μm≤bottom surface is square
It is≤4000 μm long, 50 μm≤cuboid chamber height≤80 μm.
The size of above-mentioned filtrate collection chamber 1-3 are as follows: the bottom surface side of the chamber for the cuboid that 2000 μm≤bottom surface is square
It is≤4000 μm long, 50 μm≤cuboid chamber height≤80 μm.
The above-mentioned filter membrane 1-2 for separating sample filtering room 1-1 and filtrate collection chamber 1-3, aperture is 20 μm and and sample
Product inlet chamber seamless adhesion.
The width of above-mentioned filtrate collection chamber 1-3 side wall filtrate collection chamber outlet 1-5 is 600 ± 6 μm, is highly 30-50 μm.
Above-mentioned sample export 3 include sample export room 3-2, sample export chamber inlet 3-1, sample export chamber inlet 3-1
In on the annular sidewall of cylindrical chamber.
The size of above-mentioned sample export room 3-2 are as follows: the bottom surface side of the chamber for the cuboid that 2000 μm≤bottom surface is square
Long≤4000 μm of 100 μm≤cuboid chamber height≤160 μm.
The width that above-mentioned sample export room sidewall sample exports chamber inlet 3-1 is 600 ± 6 μm, is highly 30-50 μm.
As shown in Figure 1, sample detection unit 2 be sequentially connected with the sample inlet 1 and sample export 3 it is logical.
Sample detection is carried out greater than the sample inlet 1 of the micro-fluidic chip of 20 μm of impurity with the diameter that intercepts of the invention
When, as shown in Figure 1, successively by the sample inlet 1 of micro-fluidic chip of the invention and sample detection unit 2 and sample export 3
Sample to be tested can be detected after being connected.
As shown in Figs. 1-5, after sample to be tested is injected from the entrance 1-4 at the top of the 1-1 of sample filtering room, due to sample mistake
The filtration of the bottom filter chamber 1-1 filter membrane 1-2, impurity of the diameter greater than 20 μm will be intercepted in the 1-1 of sample filtering room,
Remaining filtrate comes together in filtrate collection chamber 1-3 and exports 1-5 by filtrate collection chamber and flows into after sample detection unit 2 detected
It is flowed out through sample export 3.
So as it can be seen that micro-fluidic chip of the invention can not only intercept the impurity of diameter in test sample greater than 20 μm but also not
Detection is influenced, while structure is simple, at low cost, there is positive social effect.
Above-described embodiment is not for limitation of the invention, and the present invention is not limited only to above-described embodiment, as long as meeting
The present invention claims all belong to the scope of protection of the present invention.
Claims (7)
1. the unidirectional built-in filter membrane micro-fluidic chip that one kind can intercept impurity, it is characterised in that it is made of substrate and filter membrane bonding,
It is provided with the " Kan " shape chamber of both ends open in substrate, which includes sample inlet, sample detection unit, sample export, described
Sample inlet is sequentially connected logical with sample detection unit and sample export；
The sample inlet is located at a wherein arm for substrate " Kan " shape chamber, specifically includes sample filtering room, filtrate collection chamber, filter
Film is constituted；Sample filtering chamber inlet is located at sample filtering ceiling portion, and the bottom of sample filtering room and the entrance of filtrate collection chamber connect
Logical, the bottom of sample filtering room and the entrance opening dimension of filtrate collection chamber are consistent, and are separated between the two by filter membrane；Filtrate collection chamber
Outlet the side wall of substrate " Kan " shape chamber arm, and the entrance phase of the outlet of filtrate collection chamber and sample detection unit are set
Connection；
The sample export of above-mentioned micro-fluidic chip is located at another arm of substrate " Kan " shape chamber, specifically includes sample export room, sample
Product export chamber inlet；Sample export chamber inlet is located at the side wall of substrate " Kan " shape chamber arm, and goes out with sample detection unit
Mouth is connected.
2. the unidirectional built-in filter membrane micro-fluidic chip that one kind as described in claim 1 can intercept impurity, it is characterised in that miniflow
The sample filtering chamber room for controlling chip is cuboid, and bottom surface is square；2000 μm≤square bottom surface side length≤4000 μm, 50
μm≤cuboid chamber height≤80 μm.
3. the unidirectional built-in filter membrane micro-fluidic chip that one kind as described in claim 1 can intercept impurity, it is characterised in that miniflow
The filtrate collection chamber for controlling chip is cuboid, and bottom surface is square；2000 μm≤square bottom surface side length≤4000 μm, 50 μm≤
Cuboid chamber height≤80 μm.
4. the unidirectional built-in filter membrane micro-fluidic chip that one kind as described in claim 1 can intercept impurity, it is characterised in that miniflow
The filter membrane for separating sample filtering room and filtrate collection chamber in control chip, aperture are 20 μm and seamless with sample inlet chamber
Bonding.
5. the unidirectional built-in filter membrane micro-fluidic chip that one kind as described in claim 1 can intercept impurity, it is characterised in that miniflow
The width for controlling the filtrate collection chamber side wall filtrate collection chamber outlet of chip is 594-606 μm, is highly 30-50 μm.
6. the unidirectional built-in filter membrane micro-fluidic chip that one kind as described in claim 1 can intercept impurity, it is characterised in that miniflow
The sample export room for controlling chip is cuboid, and bottom surface is square, 2000 μm≤square bottom surface side length≤4000 μm, 100 μm
≤ cuboid chamber height≤160 μm.
7. the unidirectional built-in filter membrane micro-fluidic chip that one kind as described in claim 1 can intercept impurity, it is characterised in that miniflow
The width for controlling the sample export room sidewall sample outlet chamber inlet of chip is 594-606 μm, is highly 30-50 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811430893.8A CN109351373A (en) | 2018-11-28 | 2018-11-28 | A kind of unidirectional built-in filter membrane micro-fluidic chip intercepting impurity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811430893.8A CN109351373A (en) | 2018-11-28 | 2018-11-28 | A kind of unidirectional built-in filter membrane micro-fluidic chip intercepting impurity |
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| Publication Number | Publication Date |
|---|---|
| CN109351373A true CN109351373A (en) | 2019-02-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811430893.8A Withdrawn CN109351373A (en) | 2018-11-28 | 2018-11-28 | A kind of unidirectional built-in filter membrane micro-fluidic chip intercepting impurity |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109991195A (en) * | 2019-04-24 | 2019-07-09 | 浙江警察学院 | A kind of SPR detector single channel micro-fluidic chip clamping system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106488980A (en) * | 2014-05-15 | 2017-03-08 | 罗伯特·博世有限公司 | Process the apparatus and method of Biosample and the analysis system of analysis Biosample |
| CN107715930A (en) * | 2017-09-22 | 2018-02-23 | 华中科技大学同济医学院附属协和医院 | Chip structure |
-
2018
- 2018-11-28 CN CN201811430893.8A patent/CN109351373A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106488980A (en) * | 2014-05-15 | 2017-03-08 | 罗伯特·博世有限公司 | Process the apparatus and method of Biosample and the analysis system of analysis Biosample |
| CN107715930A (en) * | 2017-09-22 | 2018-02-23 | 华中科技大学同济医学院附属协和医院 | Chip structure |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109991195A (en) * | 2019-04-24 | 2019-07-09 | 浙江警察学院 | A kind of SPR detector single channel micro-fluidic chip clamping system |
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Application publication date: 20190219 |
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