CN109395789A - A kind of two-way built-in filter membrane micro-fluidic chip intercepting impurity - Google Patents

A kind of two-way built-in filter membrane micro-fluidic chip intercepting impurity Download PDF

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Publication number
CN109395789A
CN109395789A CN201811430865.6A CN201811430865A CN109395789A CN 109395789 A CN109395789 A CN 109395789A CN 201811430865 A CN201811430865 A CN 201811430865A CN 109395789 A CN109395789 A CN 109395789A
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China
Prior art keywords
sample
filtrate collection
collection chamber
chamber
filter membrane
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Withdrawn
Application number
CN201811430865.6A
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Chinese (zh)
Inventor
姚伟宣
王继业
吕云平
王学军
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Zhejiang Police College
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Zhejiang Police College
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Priority to CN201811430865.6A priority Critical patent/CN109395789A/en
Publication of CN109395789A publication Critical patent/CN109395789A/en
Withdrawn legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The present invention discloses a kind of two-way built-in filter membrane micro-fluidic chip that can intercept impurity.The micro-fluidic chip is made of substrate and filter membrane bonding, the " Kan " shape chamber of both ends open is provided in substrate, the chamber includes the first sample entrance, sample detection unit, the second sample entrance, and the first sample entrance is sequentially connected logical with sample detection unit and the second sample entrance.Micro-fluidic chip sample inlet export structure of the invention is simple, sample inlet and outlet can intercept the impurity in sample greater than 20 μm and remaining substance is allowed to pass through, the probability of false positive in detection process is reduced, sample inlet and outlet are used interchangeably, and will not influence testing result.

Description

A kind of two-way built-in filter membrane micro-fluidic chip intercepting impurity
Technical field
The invention belongs to micro-fluidic chips, and the sample inlet of 20 μm or more impurity can be intercepted more particularly to a kind of sample inlet Micro-fluidic chip is used interchangeably with outlet.
Background technique
Micro-fluidic chip in the market includes sample inlet, sample detection unit and sample export, this micro-fluidic chip Sample inlet cannot intercept the impurity in measuring samples, due to that can contain impurity in measuring samples, special interception need to be set Unit intercepts the impurity in measuring samples, thus this microfluidic chip structure is complicated.
Summary of the invention
The object of the present invention is to provide a kind of structure is simple, the sample that diameter in measuring samples is greater than 20 μm of impurity can be intercepted Entrance and exit is used interchangeably micro-fluidic chip.
To achieve the goals above, the technical scheme is that
A kind of micro-fluidic chip is made of substrate and filter membrane bonding, the " Kan " shape chamber of both ends open is provided in substrate, should Chamber includes the first sample entrance, sample detection unit, the second sample entrance, and the first sample entrance and sample are examined Survey unit and the second sample entrance are sequentially connected logical；The sample detection unit is existing mature detection module.
The first sample entrance is located at a wherein arm for substrate " Kan " shape chamber, specifically includes the first sample filtering Room, the first filter membrane, the first filtrate collection chamber；First sample filtering chamber inlet is located at the first sample filtering ceiling portion, the first sample The bottom of filter chamber is connected to the entrance of the first filtrate collection chamber, bottom and the first filtrate collection chamber of the first sample filtering room Entrance opening dimension is consistent, and is separated between the two by the first filter membrane；The outlet of first filtrate collection chamber is arranged in substrate " Kan " shape chamber The side wall (i.e. in the cavity wall of filtrate collection chamber) of room arm, and one end of the outlet of the first filtrate collection chamber and sample detection unit It is connected；
The sample filtering room of above-mentioned micro-fluidic chip is cuboid, and bottom surface is square；2000 μm≤bottom surface side length≤ 4000 μm, 50 μm≤cuboid chamber height≤80 μm.
The filtrate collection chamber of above-mentioned micro-fluidic chip is cuboid, and bottom surface is square；2000 μm≤bottom surface side length≤ 4000 μm, 50 μm≤cuboid chamber height≤80 μm.
The filter membrane for separating sample filtering room and filtrate collection chamber in above-mentioned micro-fluidic chip, aperture be 20 μm and with Sample inlet chamber seamless adhesion.
The width of the filtrate collection chamber side wall filtrate collection chamber outlet of above-mentioned micro-fluidic chip is 594-606 μm, is highly 30-50μm。
The second sample entrance is located at another arm of substrate " Kan " shape chamber, specifically includes the second sample filtering room, Second filter membrane, the second filtrate collection chamber；Second sample filtering chamber inlet is located at the second sample filtering ceiling portion, the second sample filtering The bottom of room is connected to the entrance of the second filtrate collection chamber, the bottom of the second sample filtering room and the entrance of the second filtrate collection chamber Size is consistent, and is separated between the two by the second filter membrane；The outlet of second filtrate collection chamber is arranged in substrate " Kan " shape chamber arm The side wall (i.e. in the cavity wall of filtrate collection chamber) in portion, and the other end phase of the outlet of the second filtrate collection chamber and sample detection unit Connection；
First sample filtering room, the first filtrate collection chamber and corresponding second sample filtering room, the second filtrate collection chamber ruler Very little size is identical.
Benefit of the invention is that: 1), the sample filtering room bottom of sample inlet setting aperture be 20 μm of filter membrane, can intercept It is greater than 20 μm of impurity in sample to be tested, structure is simple, and cost is extremely low；2), filtrate collection chamber is located at sample filtering room bottom, Impurity in sample greater than 20 μm is intercepted in sample filtering room and filtrate then flows into filtrate collection chamber, therefore this micro-fluidic Chip sample entrance impurity in buffer belt test sample greater than 20 μm and can allow remaining substance to flow into filtrate collection chamber outlet；3) Sample export structure is identical as sample inlet, can overturn use, easy to operate.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of embodiment of micro-fluidic chip of the invention；
Fig. 2 is the top view of micro-fluidic chip of the invention；
Fig. 3 is the stereoscopic schematic diagram of micro-fluidic chip of the invention；
Fig. 4 is the top view of sample entrance port；
Fig. 5 is the side view of sample entrance port.
Marked in the figure: the first sample entrance 1, the first sample filtering room 1-1, the first filter membrane 1-2, the first filtrate collection chamber 1-3, the first sample filtering chamber inlet 1-4, the first filtrate collection chamber export 1-5, sample detection unit 2, the second sample entrance 3, the second filtrate collection chamber exports 3-1, the second sample filtering room 3-2, the second sample filtering chamber inlet 3-3, and the second filtrate is collected Room 3-5, the second filter membrane 3-4.
Specific embodiment
Below in conjunction with attached drawing and the embodiment provided, the present invention is further illustrated.
As shown in Figs. 1-5, a kind of micro-fluidic chip for intercepting diameter and being greater than 20 μm of impurity, is bonded structure by substrate and filter membrane At, the " Kan " shape chamber of both ends open is provided in substrate, which includes the first sample entrance 1 and the second sample entrance 3, The first sample entrance 1 is connected with the second sample entrance 3；
The first sample entrance 1 includes the first sample filtering room 1-1, the first filter membrane 1-2, the first filtrate collection chamber 1- 3, the first sample filtering chamber inlet 1-4 and the first filtrate collection chamber export 1-5.First sample filtering chamber inlet 1-4 is located at first At the top of the 1-1 of sample filtering room, the first filtrate collection chamber outlet 1-5 is located at the first filtrate collection chamber 1-3 side wall.
First sample filtering room 1-1 and the first filtrate collection chamber 1-3 is located at the same chamber of the first sample entrance 1 In, centre is separated by the first filter membrane 1-2.
The size of above-mentioned first sample filtering room 1-1 are as follows: the bottom of the chamber for the cuboid that 2000 μm≤bottom surface is square Face side length≤4000 μm, 50 μm≤cuboid chamber height≤80 μm.
The size of above-mentioned first filtrate collection chamber 1-3 are as follows: the bottom of the chamber for the cuboid that 2000 μm≤bottom surface is square Face side length≤4000 μm, 50 μm≤cuboid chamber height≤80 μm.
Above-mentioned the first filter membrane 1-2 for separating the first sample filtering room 1-1 and the first filtrate collection chamber 1-3, aperture are equal For 20 μm and with sample inlet chamber seamless adhesion.
The width that the first filtrate collection chamber of above-mentioned first filtrate collection chamber 1-3 side wall exports 1-5 is 600 ± 6 μm, is highly 30-50μm。
Above-mentioned second sample entrance 3 includes the second sample filtering room 3-2, the second filtrate collection chamber 3-5, the second filter membrane 3- 4, the second sample filtering chamber inlet 3-3, the second filtrate collection chamber export 3-1.Second sample filtering chamber inlet 3-3 is located at the second sample At the top of product filter chamber 3-2, the second filtrate collection chamber outlet 3-1 is located on the side wall of rectangular fluid chamber.
First sample filtering room of above-mentioned micro-fluidic chip sample export, the first filtrate collection chamber, the first filter membrane, the first sample Product filter corresponding with sample inlet the second sample filtering room of chamber outlet structure size, the second filtrate collection chamber, the second filter membrane, The structure of second filtrate collection chamber outlet is identical with size.
As shown in Figure 1, sample detection unit 2 and the first sample entrance 1 and the second sample entrance 3 successively phase Connection.
Sample is carried out greater than the first sample entrance 1 of the micro-fluidic chip of 20 μm of impurity with the diameter that intercepts of the invention When detection, as shown in Figure 1, by the first sample entrance 1 of micro-fluidic chip of the invention and sample detection unit 2 and second Sample entrance 3 be sequentially connected it is logical after sample to be tested can be detected.
As shown in Figs. 1-5, when sample to be tested is from the first sample filtering chamber inlet 1- at the top of the first sample filtering room 1-1 After 4 injections, due to the filtration of the first sample filtering room bottom 1-1 the first filter membrane 1-2, impurity of the diameter greater than 20 μm will It is intercepted in the first sample filtering room 1-1, remaining filtrate comes together in the first filtrate collection chamber 1-3 and received by the first filtrate Collection room outlet 1-5 is flowed into after detection unit 2 is detected to flow out through the second sample entrance 3.
So as it can be seen that micro-fluidic chip of the invention can not only intercept the impurity of diameter in test sample greater than 20 μm but also not Detection is influenced, while structure is simple, at low cost, sample inlet and outlet are used interchangeably, and are had the characteristics that simple and efficient.
Above-described embodiment is not for limitation of the invention, and the present invention is not limited only to above-described embodiment, as long as meeting The present invention claims all belong to the scope of protection of the present invention.

Claims (6)

1. the two-way built-in filter membrane micro-fluidic chip that one kind can intercept impurity, it is characterised in that it is made of substrate and filter membrane bonding, The " Kan " shape chamber of both ends open is provided in substrate, which includes the first sample entrance, sample detection unit, the second sample Entrance, the first sample entrance are sequentially connected logical with sample detection unit and the second sample entrance；
The first sample entrance is located at a wherein arm for substrate " Kan " shape chamber, specifically includes the first sample filtering room, the One filter membrane, the first filtrate collection chamber；First sample filtering chamber inlet is located at the first sample filtering ceiling portion, the first sample filtering room Bottom be connected to the entrance of the first filtrate collection chamber, the entrance ruler of the bottom of the first sample filtering room and the first filtrate collection chamber It is very little consistent, and separated between the two by the first filter membrane；The outlet of first filtrate collection chamber is arranged in substrate " Kan " shape chamber arm Side wall, and the outlet of the first filtrate collection chamber is connected with one end of sample detection unit；
The second sample entrance is located at another arm of substrate " Kan " shape chamber, specifically includes the second sample filtering room, and second Filter membrane, the second filtrate collection chamber；Second sample filtering chamber inlet is located at the second sample filtering ceiling portion, the second sample filtering room Bottom is connected to the entrance of the second filtrate collection chamber, the bottom of the second sample filtering room and the entrance opening dimension of the second filtrate collection chamber Unanimously, and between the two it is separated by the second filter membrane；Substrate " Kan " shape chamber arm is arranged in the outlet of second filtrate collection chamber Side wall, and the outlet of the second filtrate collection chamber is connected with the other end of sample detection unit.
2. the two-way built-in filter membrane micro-fluidic chip that one kind as described in claim 1 can intercept impurity, it is characterised in that above-mentioned The sample filtering room of micro-fluidic chip is cuboid, and bottom surface is square；2000 μm≤bottom surface side length≤4000 μm, 50 μm≤long Cube chamber height≤80 μm.
3. the two-way built-in filter membrane micro-fluidic chip that one kind as described in claim 1 can intercept impurity, it is characterised in that above-mentioned The filtrate collection chamber of micro-fluidic chip is cuboid, and bottom surface is square；2000 μm≤bottom surface side length≤4000 μm, 50 μm≤long Cube chamber height≤80 μm.
4. the two-way built-in filter membrane micro-fluidic chip that one kind as described in claim 1 can intercept impurity, it is characterised in that above-mentioned The filter membrane for separating sample filtering room and filtrate collection chamber in micro-fluidic chip, aperture be 20 μm and with sample inlet chamber Seamless adhesion.
5. the two-way built-in filter membrane micro-fluidic chip that one kind as described in claim 1 can intercept impurity, it is characterised in that above-mentioned The width of the filtrate collection chamber side wall filtrate collection chamber outlet of micro-fluidic chip is 594-606 μm, is highly 30-50 μm.
6. the two-way built-in filter membrane micro-fluidic chip that one kind as described in claim 1 can intercept impurity, it is characterised in that first Sample filtering room, the first filtrate collection chamber are identical as corresponding second sample filtering room, the second filtrate collection chamber size.
CN201811430865.6A 2018-11-28 2018-11-28 A kind of two-way built-in filter membrane micro-fluidic chip intercepting impurity Withdrawn CN109395789A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106488980A (en) * 2014-05-15 2017-03-08 罗伯特·博世有限公司 Process the apparatus and method of Biosample and the analysis system of analysis Biosample
CN107715930A (en) * 2017-09-22 2018-02-23 华中科技大学同济医学院附属协和医院 Chip structure

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106488980A (en) * 2014-05-15 2017-03-08 罗伯特·博世有限公司 Process the apparatus and method of Biosample and the analysis system of analysis Biosample
CN107715930A (en) * 2017-09-22 2018-02-23 华中科技大学同济医学院附属协和医院 Chip structure

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Application publication date: 20190301