CN109350672A - A kind of nanometer of tea oil fatty acid calcium iron zinc complexes and its preparation method and application - Google Patents
A kind of nanometer of tea oil fatty acid calcium iron zinc complexes and its preparation method and application Download PDFInfo
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- CN109350672A CN109350672A CN201710929764.2A CN201710929764A CN109350672A CN 109350672 A CN109350672 A CN 109350672A CN 201710929764 A CN201710929764 A CN 201710929764A CN 109350672 A CN109350672 A CN 109350672A
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- fatty acid
- tea oil
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- oil fatty
- zinc
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- 239000010495 camellia oil Substances 0.000 title claims abstract description 69
- 235000014113 dietary fatty acids Nutrition 0.000 title claims abstract description 61
- 239000000194 fatty acid Substances 0.000 title claims abstract description 61
- 229930195729 fatty acid Natural products 0.000 title claims abstract description 61
- -1 fatty acid calcium iron zinc complexes Chemical class 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 32
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 19
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 239000003921 oil Substances 0.000 claims abstract description 17
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 17
- 239000011701 zinc Substances 0.000 claims abstract description 17
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000011575 calcium Substances 0.000 claims abstract description 16
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 16
- 229910052742 iron Inorganic materials 0.000 claims abstract description 16
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 15
- 238000013517 stratification Methods 0.000 claims abstract description 15
- 102000004882 Lipase Human genes 0.000 claims abstract description 14
- 108090001060 Lipase Proteins 0.000 claims abstract description 14
- 239000004367 Lipase Substances 0.000 claims abstract description 13
- 235000019421 lipase Nutrition 0.000 claims abstract description 13
- JYMOQWNEIZBVFC-UHFFFAOYSA-N [Fe].[Zn].[Ca] Chemical compound [Fe].[Zn].[Ca] JYMOQWNEIZBVFC-UHFFFAOYSA-N 0.000 claims abstract description 12
- 235000002639 sodium chloride Nutrition 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract description 9
- 239000012528 membrane Substances 0.000 claims abstract description 9
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 239000011780 sodium chloride Substances 0.000 claims abstract description 9
- 229960002668 sodium chloride Drugs 0.000 claims abstract description 9
- 159000000007 calcium salts Chemical class 0.000 claims abstract description 5
- 150000003751 zinc Chemical class 0.000 claims abstract description 5
- 208000024172 Cardiovascular disease Diseases 0.000 claims abstract description 4
- 208000026106 cerebrovascular disease Diseases 0.000 claims abstract description 4
- 239000003814 drug Substances 0.000 claims abstract description 4
- 230000002526 effect on cardiovascular system Effects 0.000 claims abstract description 4
- 229940079593 drug Drugs 0.000 claims abstract description 3
- 235000013402 health food Nutrition 0.000 claims abstract description 3
- 230000002265 prevention Effects 0.000 claims abstract description 3
- 239000013589 supplement Substances 0.000 claims abstract description 3
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 8
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 6
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 4
- 239000004277 Ferrous carbonate Substances 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 claims description 4
- 239000000292 calcium oxide Substances 0.000 claims description 4
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 4
- RAQDACVRFCEPDA-UHFFFAOYSA-L ferrous carbonate Chemical compound [Fe+2].[O-]C([O-])=O RAQDACVRFCEPDA-UHFFFAOYSA-L 0.000 claims description 4
- 235000019268 ferrous carbonate Nutrition 0.000 claims description 4
- 229960004652 ferrous carbonate Drugs 0.000 claims description 4
- 239000011790 ferrous sulphate Substances 0.000 claims description 4
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 4
- 229910000015 iron(II) carbonate Inorganic materials 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 3
- 239000011592 zinc chloride Substances 0.000 claims description 3
- 235000005074 zinc chloride Nutrition 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical group [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229960001763 zinc sulfate Drugs 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 241001122767 Theaceae Species 0.000 claims description 2
- 238000004321 preservation Methods 0.000 abstract description 7
- 238000006243 chemical reaction Methods 0.000 abstract description 6
- 238000003756 stirring Methods 0.000 abstract description 4
- 206010048259 Zinc deficiency Diseases 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 235000019198 oils Nutrition 0.000 description 12
- 239000002105 nanoparticle Substances 0.000 description 6
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 3
- 229940091251 zinc supplement Drugs 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid group Chemical group S(O)(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- 206010013883 Dwarfism Diseases 0.000 description 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 1
- 239000008108 microcrystalline cellulose Substances 0.000 description 1
- 229940016286 microcrystalline cellulose Drugs 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 208000005368 osteomalacia Diseases 0.000 description 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000011122 softwood Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 239000011667 zinc carbonate Substances 0.000 description 1
- 235000004416 zinc carbonate Nutrition 0.000 description 1
- 229910000010 zinc carbonate Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/26—Iron; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/30—Zinc; Compounds thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
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Abstract
The invention discloses a kind of nanometer of tea oil fatty acid calcium iron zinc complexes and its preparation method and application.This nanometer of tea oil fatty acid calcium iron zinc complexes each component percentage composition by weight are as follows: tea oil fatty acid 80~95%, calcium 3~18%, zinc 0.05~1%, iron 0.05~1%.The step of preparation method are as follows: immobilized lipase, heat preservation a period of time and stratification are added in tea oil, takes upper layer oil liquid；It is eluted with sodium-chloride water solution, stratification, retains upper layer oil liquid；Calcium salt, zinc salt and ferrous salt is added, stirs lower reaction；The filter membrane of certain size is crossed, is freeze-dried, obtains a nanometer tea oil fatty acid calcium iron zinc complexes.Its health food or drug for can be used for preparing supplement calcium iron zinc deficiency, prevention cardiovascular and cerebrovascular disease.Nanometer tea oil fatty acid calcium iron zinc complexes of the invention had not only improved the stability of fatty acid, but also promoted being absorbed and utilized for calcium iron zinc, efficiently use calcium iron zinc can, and were not the absorption that influences each other.
Description
Technical field
The invention belongs to field of medicaments, and in particular to a kind of nanometer of tea oil fatty acid calcium iron zinc complexes and preparation method thereof
And application.
Background technique
Calcium, iron and zinc are element needed by human, are even more important to the development of children.Lacking calcium, iron and zinc can lead to
Osteomalacia, anemia and nanism.The partial eclipse of children, few food are likely to result in the shortage of calcium, iron and zinc.Current common calcium,
The replenishers of iron and zinc are gluconic acid conjugate or are inorganic salts, but to be directly absorbed by the body utilization rate low for inorganic salts, and Portugal
The intake of grape sugar is excessively in addition to it can lead to obesity, induced Diabetic, saprodontia is promoted to occur, it is also possible to influence intelligence.
Tea oil degree of unsaturation is high, containing polyunsaturated fatty acid, referred to as " east olive oil ".Polyunsaturated fatty acid
Vascular patency can be maintained, cardiovascular and cerebrovascular disease is prevented and treated.But polyunsaturated fatty acid is easily oxidized, and stability is poor, with metal from
Son, which combines, can be improved its stability.And tea oil fatty acid is reacted with calcium, iron and zinc and prepares tea oil fatty acid calcium iron zinc complexes
Method as well as organic calcium, iron, zinc replenishers application have not been reported.
Summary of the invention
(1) technical problems to be solved
In view of the deficiencies of the prior art, the technical problem to be solved in the present invention is to provide a kind of nanometer of tea oil fatty acid calcium iron
Zinc complexes, the present invention also provides the preparation methods and this nanometer of tea oil of this nanometer of tea oil fatty acid calcium iron zinc complexes
The application of fatty acid calcium iron zinc complexes.
(2) technical solution
In order to solve the above-mentioned technical problems, the present invention provides such a nanometer of tea oil fatty acid calcium iron zinc complexes,
Wherein each component percentage composition by weight are as follows: tea oil fatty acid 80~95%, calcium 3~18%, zinc 0.05~1%, iron 0.05~
1%.
The present invention also provides the preparation methods of above-mentioned a kind of nanometer of tea oil fatty acid calcium iron zinc complexes, including walk as follows
It is rapid:
A. the immobilized lipase that active unit is 2000~10000U is added in every kilogram of tea oil, is protected at 40~50 DEG C
3~8h of temperature, stratification take upper layer oil liquid；
B. it is eluted, is stood with the sodium-chloride water solution that quality is 0.5~3 times of tea oil, mass percentage concentration is 0.1~1%
Layering retains upper layer oil liquid；
C. calcium salt, zinc salt and the ferrous salt of formula ratio is added, is stirred under 5000~20000 revs/min, it is anti-at 50~70 DEG C
Answer 1~3h；
D. the filter membrane of 0.22~0.45m is crossed, is freeze-dried, obtains a nanometer tea oil fatty acid calcium iron zinc complexes.
Preferably, in step, immobilized lipase is the lipase that every gram of enzyme activity unit is greater than 100000.
Preferably, in step C, calcium salt is any one in calcium oxide, calcium chloride or calcium nitrate；Zinc salt is sulfuric acid
Any one in zinc, zinc chloride or zinc nitrate；Ferrous salt is any one in ferrous sulfate, frerrous chloride or ferrous carbonate
Kind.
The present invention also provides the applications of above-mentioned a kind of nanometer of tea oil fatty acid calcium iron zinc complexes, are used to prepare supplement
Calcium iron zinc is insufficient, the health food or drug of prevention cardiovascular and cerebrovascular disease.
The principle of the present invention: calcium, iron, zinc form organic calcium iron zinc, and further nanosizing in conjunction with tea oil fatty acid,
The stable nanometer tea oil fatty acid calcium iron zinc complexes of quality can be formed.
(3) beneficial effect
The present invention has the following advantages and effects with respect to the prior art:
1. nanometer tea oil fatty acid calcium iron zinc complexes partial size of the invention both improves fatty acid in 200~500nm
Stability, and being absorbed and utilized for calcium iron zinc is promoted, using proportion of the invention, it can make calcium iron zinc that can efficiently use, without
It causes to absorb in influencing each other；
2. the present invention uses immobilized lipase enzyme hydrolysis tea oil for fatty acid, specificity is stronger, while being easily isolated and following
Ring utilizes；
3. preparation process of the present invention is simple, reaction condition is mild, is convenient for industrialized production.
Specific embodiment
It, below will be right to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention
Technical solution in the embodiment of the present invention carries out clear, complete description, with the present invention is further explained, it is clear that described
Embodiment is only a part of the embodiments of the present invention, instead of all the embodiments.
Embodiment 1
A kind of nanometer of tea oil fatty acid calcium iron zinc complexes, wherein each component percentage composition by weight are as follows: tea oil fatty acid
80%, calcium 18%, zinc 1%, iron 1%.
The preparation method of above-mentioned nanometer tea oil fatty acid calcium iron zinc complexes: take 1kg tea oil that active unit 10000U is added
Immobilized lipase, 40 DEG C of heat preservation 3h；Stratification takes upper layer oil liquid；It is eluted with 0.1% sodium-chloride water solution of 3kg,
Stratification retains upper layer oil liquid；Calcium oxide, zinc sulfate and the ferrous sulfate of formula ratio is added, 5000 turns/min is stirred, and 50 DEG C
React 3h；The filter membrane of 0.22m is crossed, is freeze-dried, obtains a nanometer tea oil fatty acid calcium iron zinc complexes 810g.
It is measured through nano particle size instrument, the partial size of above-mentioned nanometer tea oil fatty acid calcium iron zinc complexes is 260 ± 18nm.
Embodiment 2
A kind of nanometer of tea oil fatty acid calcium iron zinc complexes, wherein each component percentage composition by weight are as follows: tea oil fatty acid
95%, calcium 3%, zinc 1%, iron 1%.
The preparation method of above-mentioned nanometer tea oil fatty acid calcium iron zinc complexes: 1kg tea oil is taken to be added active unit 2000U's
Immobilized lipase, 50 DEG C of heat preservation 8h；Stratification takes upper layer oil liquid；It is eluted with 1% sodium-chloride water solution of 0.5kg, it is quiet
Layering is set, upper layer oil liquid is retained；Calcium chloride, zinc chloride and the frerrous chloride of formula ratio is added, 20000 turns/min is stirred, and 70 DEG C
React 1h；The filter membrane of 0.45m is crossed, is freeze-dried, obtains a nanometer tea oil fatty acid calcium iron zinc complexes 730g.
It is measured through nano particle size instrument, the partial size of above-mentioned nanometer tea oil fatty acid calcium iron zinc complexes is 470 ± 32nm.
Embodiment 3
A kind of nanometer of tea oil fatty acid calcium iron zinc complexes, wherein each component percentage composition by weight are as follows: tea oil fatty acid
90%, calcium 9.9%, zinc 0.05%, iron 0.05%.
Take 1kg tea oil that the immobilized lipase of active unit 5000U, 45 DEG C of heat preservation 5h are added；Stratification takes upper layer oily
Liquid；It is eluted with 0.5% sodium-chloride water solution of 2kg, stratification retains upper layer oil liquid；Calcium nitrate, the nitre of formula ratio is added
Sour zinc and ferrous carbonate, 10000 turns/min stirring, 60 DEG C of reaction 2h；The filter membrane of 0.3m is crossed, is freeze-dried, obtains a nanometer tea oil
Fatty acid calcium iron zinc complexes 773g.
It is measured through nano particle size instrument, the partial size of above-mentioned nanometer tea oil fatty acid calcium iron zinc complexes is 353 ± 21nm.
Embodiment 4
A kind of nanometer of tea oil fatty acid calcium iron zinc complexes, wherein each component percentage composition by weight are as follows: tea oil fatty acid
85%, calcium 14.9%, zinc 0.05%, iron 0.05%.
Take 1kg tea oil that the immobilized lipase of active unit 8000U, 50 DEG C of heat preservation 4h are added；Stratification takes upper layer oily
Liquid；It is eluted with 0.7% sodium-chloride water solution of 2.5kg, stratification retains upper layer oil liquid；The calcium oxide of addition formula ratio,
Zinc sulfate and ferrous sulfate, 15000 turns/min stirring, 55 DEG C of reaction 1.5h；The filter membrane of 0.4m is crossed, is freeze-dried, obtains nanometer
Tea oil fatty acid calcium iron zinc complexes 754g.
It is measured through nano particle size instrument, the partial size of above-mentioned nanometer tea oil fatty acid calcium iron zinc complexes is 284 ± 19nm.
Embodiment 5
A kind of nanometer of tea oil fatty acid calcium iron zinc complexes, wherein each component percentage composition by weight are as follows: tea oil fatty acid
82%, calcium 17%, zinc 0.5%, iron 0.5%.
Take 1kg tea oil that the immobilized lipase of active unit 4000U, 50 DEG C of heat preservation 7h are added；Stratification takes upper layer oily
Liquid；It is eluted with 0.5% sodium-chloride water solution of 1kg, stratification retains upper layer oil liquid；Calcium chloride, the chlorine of formula ratio is added
Change zinc and frerrous chloride, 70000 turns/min stirring, 65 DEG C of reaction 2.5h；The filter membrane of 0.22m is crossed, is freeze-dried, obtains a nanometer tea
Fatty acid oil calcium iron zinc complexes 685g.
It is measured through nano particle size instrument, the partial size of above-mentioned nanometer tea oil fatty acid calcium iron zinc complexes is 316 ± 26nm.
Embodiment 6
A kind of nanometer of tea oil fatty acid calcium iron zinc complexes, wherein each component percentage composition by weight are as follows: tea oil fatty acid
94%, calcium 5%, zinc 0.5%, iron 0.5%.
Take 1kg tea oil that the immobilized lipase of 2000~10000U of active unit, 40~50 DEG C of 3~8h of heat preservation are added；It is quiet
Layering is set, upper layer oil liquid is taken；It is eluted with 0.1~1% sodium-chloride water solution of 0.5~3kg, stratification, retains upper layer oil
Liquid；Calcium nitrate, zinc nitrate and the ferrous carbonate of formula ratio is added, 5000~20000 turns/min is stirred, 50~70 DEG C of reactions 1~
3h；The filter membrane of 0.22~0.45m is crossed, is freeze-dried, obtains a nanometer tea oil fatty acid calcium iron zinc complexes 810g.
It is measured through nano particle size instrument, the partial size of above-mentioned nanometer tea oil fatty acid calcium iron zinc complexes is 260 ± 18nm.
Embodiment 7
Soluble starch 85g, sucrose 5g is added in nanometer tea oil fatty acid calcium iron zinc complexes 10g made from embodiment 1,
It is made 10g/ bags of granule.One day one bag take after mixing it with water, can be used as calcium iron Zinc supplements.
Embodiment 8
Starch 80g, syrup 10g, softwood processed is added in nanometer tea oil fatty acid calcium iron zinc complexes 10g made from embodiment 3
And at tabletting (0.2g/ piece).One a piece of oral, can be used as calcium iron Zinc supplements.
Embodiment 9
Nanometer tea oil fatty acid calcium iron zinc complexes 10g made from embodiment 6 is added microcrystalline cellulose 90g dispersion, is made
Capsule (0.5g/).One day one it is oral, can be used as calcium iron Zinc supplements.
The foregoing describe technical characteristics of the invention and basic principle and associated advantages, for those skilled in the art
For, it is clear that invention is not limited to the details of the above exemplary embodiments, and without departing substantially from design of the invention or substantially special
In the case where sign, the present invention can be realized in other specific forms.It therefore, in all respects, should all be by embodiment
Regard exemplary as, and is non-limiting, the scope of the invention being indicated by the appended claims rather than by the foregoing description,
It is intended that including all changes that fall within the meaning and scope of the equivalent elements of the claims in the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (5)
1. a kind of nanometer of tea oil fatty acid calcium iron zinc complexes, which is characterized in that wherein each component percentage composition by weight are as follows: tea
Fatty acid oil 80~95%, calcium 3~18%, zinc 0.05~1%, iron 0.05~1%.
2. a kind of method for preparing nanometer tea oil fatty acid calcium iron zinc complexes described in claim 1, which is characterized in that including
Following steps:
A. the immobilized lipase that active unit is 2000~10000U is added in every kilogram of tea oil, keeps the temperature 3 at 40~50 DEG C
~8h, stratification take upper layer oil liquid；
B. it is eluted with the sodium-chloride water solution that quality is 0.5~3 times of tea oil, mass percentage concentration is 0.1~1%, stratification,
Retain upper layer oil liquid；
C. calcium salt, zinc salt and the ferrous salt of formula ratio is added, is stirred under 5000~20000 revs/min, reacts 1 at 50~70 DEG C
~3h；
D. the filter membrane of 0.22~0.45m is crossed, is freeze-dried, obtains a nanometer tea oil fatty acid calcium iron zinc complexes.
3. the preparation method of a kind of nanometer of tea oil fatty acid calcium iron zinc complexes according to claim 2, which is characterized in that
In step, immobilized lipase is the lipase that every gram of enzyme activity unit is greater than 100000.
4. the preparation method of a kind of nanometer of tea oil fatty acid calcium iron zinc complexes according to claim 2, which is characterized in that
In step C, calcium salt is any one in calcium oxide, calcium chloride or calcium nitrate；Zinc salt is zinc sulfate, zinc chloride or zinc nitrate
In any one；Ferrous salt is any one in ferrous sulfate, frerrous chloride or ferrous carbonate.
5. a kind of nanometer tea oil fatty acid calcium iron zinc complexes according to claim 1 and/claim 2 to 4 are any one
The application of the nanometer tea oil fatty acid calcium iron zinc complexes of item the method preparation, which is characterized in that it is used to prepare supplement calcium
Iron zinc is insufficient, the health food or drug of prevention cardiovascular and cerebrovascular disease.
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