CN109348986A - A kind of method of fermentation material sack soil covering culture vulcanized ester - Google Patents
A kind of method of fermentation material sack soil covering culture vulcanized ester Download PDFInfo
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- CN109348986A CN109348986A CN201811398234.0A CN201811398234A CN109348986A CN 109348986 A CN109348986 A CN 109348986A CN 201811398234 A CN201811398234 A CN 201811398234A CN 109348986 A CN109348986 A CN 109348986A
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- Prior art keywords
- bag
- culture
- soil covering
- fermentation
- sack
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention patent relates to fungi cultivation technical field, especially a kind of method of fermentation material sack soil covering culture vulcanized ester.The cultural method is that fermentation material soil covering culture vulcanized ester is made in raw material mixed fermentation with cotton seed hull 40%, hardwood sawdust 37%, wheat bran 10%, corn flour 5%, soyabean expeller powder 5%, pulverized limestone 3%, and key step includes: the configuration of (1) compost；(2) heap fermentation is built；(3) pack sterilizing；(4) inoculated and cultured；(5) bag soil covering culture is taken off；(6) flower bud；(7) it harvests.The method and technology of fermentation material sack soil covering culture vulcanized ester provided by the invention is simple, low in cost, yield is high, Fruiting quality is good, culture medium is made as fermenting raw materials using cotton seed hull, hardwood sawdust, wheat bran, corn flour, soyabean expeller powder and pulverized limestone, raw material is easy to get, nutrition configuration is balanced, relative to traditional raw material pouch inoculated and cultured, reduces labor intensity, production efficiency is improved, large-scale promotion application is convenient for.
Description
Technical field
The invention patent relates to fungi cultivation technical field, especially a kind of side of fermentation material sack soil covering culture vulcanized ester
Method.
Background technique
Vulcanized ester is commonly called as pear tree chicken or tree chicken mushroom, is subordinate to Basidiomycotina, Hymenomycetes, Aphyllophorales, Polyporaceae, sulphur
Pseudomonas is the more rare food medicine dual-purpose bacterium in northeast.Its fructification cap meat, lower edge it is tapered it is raw in matrix in covering watt
Shape, edge is wavy, and color is by pale yellow to vermilion, and bright in luster, delicious flavour when children is tender, mouthfeel is like salmon, old rear Cheng Gan
Junket shape, inedibility, but can be used as medicine, have the function of regulative mechanism, improves health, improves the immunity of the human body.The bacterium nutrition is rich
Richness is not only rich in a variety of essential amino acids, but also contains a large amount of eburicoic acids, research shows that eburicoic acid is synthesizing steroid medicine
The primary raw material of object has prevention and treatment effect to a variety of diseases, especially distinguishes small white mouse sarcoma 180 and ehrlich carcinoma inhibiting rate
For 80% and 90%.
Mainly there is wild and artificial culture in the source of vulcanized ester at present, wherein wild vulcanized ester due to being grown in late summer mostly
At the beginning of autumn on toothed oak wood living or rotten, low output, and be affected by season and environment, have been unable to meet growing market
Demand, therefore artificial culture vulcanized ester has become emphasis concerned by people in recent years.But make a general survey of the sulphur of current widespread reports
Bacterium training mode is the plastic pouch for using the raw material that will be stirred to be directly loadable into specification as 17 × 35 × 0.004cm mostly, two
Head inoculation, naturally stacking fruiting cultivated, this often exists, and bacterium germination speed is slow, production efficiency is low, fructification is small, low output,
The shortcomings such as poor quality, it is difficult to carry out large-scale promotion application.
Summary of the invention
For the problems of the prior art, the invention aims to provide a kind of technically simple, low in cost, yield
Height, the method for the good fermentation material sack soil covering culture vulcanized ester of quality.
In order to achieve the above object, the technical scheme is that
A kind of method of fermentation material sack soil covering culture vulcanized ester, comprising the following steps:
(1) compost configures
Prepare each raw material: cotton seed hull 40% by mass percentage, hardwood sawdust 37%, wheat bran 10%, corn flour 5%,
Cotton seed hulls and sawdust are first mixed and are mixed uniformly by soyabean expeller powder 5%, pulverized limestone 3%, add water adjust water content to 70%~
75%, it then sprinkles pulverized limestone and mixes thoroughly, accumulate the 12~16h that prewets and mix wheat bran, corn flour, soyabean expeller powder after it is impregnated with water
Compost is made in even spice.
(2) heap fermentation is built
By Compost at wide 2~2.5m, high 0.8~1m, length unlimited material heap is straight-through from heap top with wooden stick after heap
Stomata, venthole aperture 10cm, venthole interval 30cm are uniformly got through in heap bottom.When material heap 10cm depths temperature is up to 50 DEG C or more
When, it keeps for 24 hours, then carrying out a turning, when turning need to turn surface layer, centre, bottom uniformly, and heap concora crush is got through again later
Stomata, by aforesaid operations process turning 3~4 times, when last time turning, checks feed moisture content, to be held between material webs with hand-tight
There is water exudation to be advisable without lower drop, if partially dry, can be adjusted with 1% lime supernatant, if partially wet can turn over heap twice or suitable more
When extension fermentation time.5~8d of fermentation is dark brown to the material fermented, has a large amount of actinomyces albus to occur, free from extraneous odour simultaneously has
There is fermenting aroma to terminate to ferment.
(3) pack sterilizing
Fermentation material obtained above is packed into width to roll over for 20~22cm, a length of 40~42cm, with a thickness of 0.004cm polyethylene
In the back cover polybag of angle, material bag should not be excessively loose, so as not to sterilizing after deform, fill reality after sack lantern ring and without cotton lid seal, then
The material bag installed is put into normal-pressure sterilizing pot and is sterilized.
(4) inoculated and cultured
Material bag after sterilizing is cooled down, 30 DEG C or less is cooled to and opens no cotton lid in an aseptic environment and carry out one and connect
Kind, every bag of inoculum concentration is about 40g or so, and then the bacterium bag after inoculation is placed on the indoor Multilayer culture shelf of culture and is trained
It supports, 10d before cultivating, room temperature is controlled at 24 DEG C~26 DEG C, and to promote strain, cover is colonized as early as possible, since mycelia has been obturaged after 10d
Charge level simultaneously gos deep into material, and room temperature should be controlled at 22~24 DEG C, when mycelia gos deep into material bag 5cm or so, should increase room ventilation amount,
Promote mycelia robust growth, cultivates 45d or so under normal circumstances, mycelia covers with material bag.
(5) bag soil covering culture is taken off
Fruiting greenhouse is built, and greenhouse place is cleaned up, pesticide and disinfectant, is sprinkled profoundly water, is laterally or longitudinally dug on ground
Wide 1.2m, deep 20cm, length are consistent with greenhouse, and spacing is the ridge-up bed of 60cm, spread one on the ridge-up bed dug and the soil living dug out
The fresh pulverized limestone of layer reduces disease in order to improving soil pH value, and the bacterium bag for being then up to physiological maturity transports in canopy,
The plastic foil for cutting off 8~10cm of bacterium bag bottom end keeps fermentation material culture medium exposed part downward, is uprightly thrown into ridge-up bed, bacterium bag
Between distance be 3cm, bacterium bag gap filled and led up with fine earth, until bacterium bag upper end 5cm or so stops earthing, in order to avoid soil contamination is real
Body.
(6) flower bud
After ridge-up bed is well placed, removal bacterium bag upper end is cut off without cotton lid and lantern ring with rope or rubber band pine bundle sack simultaneously
Extra bag film above, control temperature of shed are 14~22 DEG C, and relative air humidity is 85%~95%, intensity of illumination 400
~600lux, the CO in environment2Concentration < 0.1%, management 10d or so, can grow button at sack.
(7) it harvests
It is Harvesting Date when cap is open and flat, spore not yet launches, 10d or so can be harvested generally after buddingging, and first
After damp fructification harvesting, stop the work of water sprinkling for better material moisture into canopy in 3~5d, pours permeable, the left side 10d into ridge-up bed after mycelia restores
The right side can form two damp buttons.
Specifically, the normal-pressure sterilization operation in the step (3) is that preceding 4h makes material bag temperature reach 80 DEG C or more, otherwise expect
Bag easily souring due to anaerobic fermentation, is continuously heating to 100 DEG C later, when material bag temperature is stablized at 100 DEG C, holding 10~
12h completes sterilizing.
Compared with prior art, the invention has the advantages that
1. the method and technology of fermentation material sack soil covering culture vulcanized ester provided by the invention is simple, low in cost, yield is high,
Fruiting quality is good, using cotton seed hull, hardwood sawdust, wheat bran, corn flour, soyabean expeller powder and pulverized limestone as fermenting raw materials system
Culture medium is obtained, raw material is easy to get, and nutrition configuration is balanced.
2. the present invention uses the big bag cultivating vulcanized ester of earthing relative to traditional raw material pouch inoculated and cultured, substantially reduce
Labor intensity, simultaneously because nutrition and the mineral element such as humus rich in, nitrogen, phosphorus, potassium in soil, can not only be sulphur
Sulphur bacterium provides complementary nutriment, and can effectively promote absorption of the bacterium bag to nutrition by watering, and after earthing
Ground temperature, humidity are more constant, are more advantageous to the formation and growth and development of fructification, therefore in the premise for guaranteeing vulcanized ester quality
Under, production efficiency is even more substantially increased, large-scale promotion application is convenient for.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described.
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1
A kind of method of fermentation material sack soil covering culture vulcanized ester, comprising the following steps:
(1) compost configures
Prepare each raw material: cotton seed hull 40% by mass percentage, hardwood sawdust 37%, wheat bran 10%, corn flour 5%,
Cotton seed hulls and sawdust are first mixed and are mixed uniformly by soyabean expeller powder 5%, pulverized limestone 3%, add water adjust water content to 70%~
75%, it then sprinkles pulverized limestone and mixes thoroughly, accumulate the 12~16h that prewets and mix wheat bran, corn flour, soyabean expeller powder after it is impregnated with water
Compost is made in even spice.
(2) heap fermentation is built
By Compost at wide 2~2.5m, high 0.8~1m, length unlimited material heap is straight-through from heap top with wooden stick after heap
Stomata, venthole aperture 10cm, venthole interval 30cm are uniformly got through in heap bottom.When material heap 10cm depths temperature is up to 50 DEG C or more
When, it keeps for 24 hours, then carrying out a turning, when turning need to turn surface layer, centre, bottom uniformly, and heap concora crush is got through again later
Stomata, by aforesaid operations process turning 3~4 times, when last time turning, checks feed moisture content, to be held between material webs with hand-tight
There is water exudation to be advisable without lower drop, if partially dry, can be adjusted with 1% lime supernatant, if partially wet can turn over heap twice or suitable more
When extension fermentation time.5~8d of fermentation is dark brown to the material fermented, has a large amount of actinomyces albus to occur, free from extraneous odour simultaneously has
There is fermenting aroma to terminate to ferment.
(3) pack sterilizing
Fermentation material obtained above is packed into width to roll over for 20~22cm, a length of 40~42cm, with a thickness of 0.004cm polyethylene
In the back cover polybag of angle, material bag should not be excessively loose, so as not to sterilizing after deform, fill reality after sack lantern ring and without cotton lid seal, then
The material bag installed is put into normal-pressure sterilizing pot and is sterilized, normal-pressure sterilization operation be preceding 4h make material bag temperature reach 80 DEG C with
On, otherwise material bag easily souring due to anaerobic fermentation, is continuously heating to 100 DEG C later, when material bag temperature is stablized at 100 DEG C,
10~12h is kept to complete sterilizing.
(3) inoculated and cultured
Material bag after sterilizing is cooled down, 30 DEG C or less is cooled to and opens no cotton lid in an aseptic environment and be inoculated with,
Every bag of inoculum concentration is about 40g or so, and then the bacterium bag after inoculation is placed on the indoor Multilayer culture shelf of culture and is cultivated,
10d before cultivating, room temperature are controlled at 24 DEG C~26 DEG C, and to promote strain, cover is colonized as early as possible, since mycelia has obturaged charge level after 10d
And go deep into material, room temperature should be controlled at 22~24 DEG C, when mycelia gos deep into material bag 5cm or so, should increase room ventilation amount, be promoted
Mycelia robust growth cultivates 45d or so under normal circumstances, and mycelia covers with material bag.
(4) bag soil covering culture is taken off
Fruiting greenhouse is built, and greenhouse place is cleaned up, pesticide and disinfectant, is sprinkled profoundly water, is laterally or longitudinally dug on ground
Wide 1.2m, deep 20cm, length are consistent with greenhouse, and spacing is the ridge-up bed of 60cm, spread one on the ridge-up bed dug and the soil living dug out
The fresh pulverized limestone of layer reduces disease in order to improving soil pH value, and the bacterium bag for being then up to physiological maturity transports in canopy,
The plastic foil for cutting off 8~10cm of bacterium bag bottom end keeps fermentation material culture medium exposed part downward, is uprightly thrown into ridge-up bed, bacterium bag
Between distance be 3cm, bacterium bag gap filled and led up with fine earth, until bacterium bag upper end 5cm or so stops earthing, in order to avoid soil contamination is real
Body.
(5) flower bud
After ridge-up bed is well placed, removal bacterium bag upper end is cut off without cotton lid and lantern ring with rope or rubber band pine bundle sack simultaneously
Extra bag film above, control temperature of shed are 14~22 DEG C, and relative air humidity is 85%~95%, intensity of illumination 400
~600lux, the CO2 concentration < 0.1% in environment, management 10d or so, can grow button at sack.
(6) it harvests
It is Harvesting Date when cap is open and flat, spore not yet launches, 10d or so can be harvested generally after buddingging, and first
After damp fructification harvesting, stop the work of water sprinkling for better material moisture into canopy in 3~5d, pours permeable, the left side 10d into ridge-up bed after mycelia restores
The right side can form two damp buttons.
The foregoing description of the disclosed embodiments can be realized those skilled in the art or using the present invention.To this
A variety of modifications of a little embodiments will be apparent for a person skilled in the art, and the general principles defined herein can
Without departing from the spirit or scope of the present invention, to realize in other embodiments.Therefore, the present invention will not be limited
It is formed on the embodiments shown herein, and is to fit to consistent with the principles and novel features disclosed in this article widest
Range.
Claims (4)
1. a kind of method of fermentation material sack soil covering culture vulcanized ester, which comprises the following steps:
(1) compost configures
Prepare each raw material: cotton seed hull 40%, hardwood sawdust 37%, wheat bran 10%, corn flour 5%, soya bean by mass percentage
Cotton seed hulls and sawdust are first mixed and are mixed uniformly by dregs of rice powder 5%, pulverized limestone 3%, are added water to adjust water content to 70%~75%, are connect
Sprinkle pulverized limestone and mix thoroughly, accumulation is prewetted 12~16h, remaining raw material is then mixed even spice, compost is made；
(2) heap fermentation is built
By Compost at wide 2~2.5m, high 0.8~1m, the unlimited material heap of length, with wooden stick from heap top straight through reactor bottom after heap
Uniformly get through stomata, venthole aperture 10cm, venthole interval 30cm are protected when material heap 10cm depths temperature is up to 50 DEG C or more
It holds for 24 hours, then carries out a turning, when turning, need to turn surface layer, centre, bottom uniformly, and heap concora crush gets through stomata again later,
By aforesaid operations process turning 3~4 times, the 5~8d that ferments is dark brown to the material fermented, has a large amount of actinomyces albus to occur,
Free from extraneous odour simultaneously there is fermenting aroma to terminate to ferment；
(3) pack sterilizing
Fermentation material obtained above is packed into width to seal for 20~22cm, a length of 40~42cm, with a thickness of 0.004cm polyethylene dog-ear
In the polybag of bottom, fill reality after sack lantern ring and without cotton lid seal, then the material bag installed is put into normal-pressure sterilizing pot and is carried out
Sterilizing；
(4) inoculated and cultured
Material bag after sterilizing is cooled down, 30 DEG C or less is cooled to and opens no one inoculation of cotton lid progress in an aseptic environment,
Every bag of inoculum concentration is 40g, and then the bacterium bag after inoculation is placed on the indoor Multilayer culture shelf of culture and is cultivated, mycelia is worked as
When going deep into material bag 5cm, room ventilation amount is increased, cultivates 45d under normal circumstances, mycelia covers with material bag；
(5) bag soil covering culture is taken off
Fruiting greenhouse is built, and greenhouse place is cleaned up, pesticide and disinfectant, is sprinkled profoundly water, laterally or longitudinally digs width on ground
1.2m, deep 20cm, length are consistent with greenhouse, and spacing is the ridge-up bed of 60cm, spread one layer on the ridge-up bed dug and the soil living dug out
Pulverized limestone, the bacterium bag for being then up to physiological maturity transport in canopy, cut off the plastic foil of 8~10cm of bacterium bag bottom end, make fermentation material
Culture medium exposed part is downward, is uprightly thrown into ridge-up bed, and distance is 3cm between bacterium bag, and bacterium bag gap is filled and led up with fine earth, until
Bacterium bag upper end 5cm stops earthing, in case soil is infected with fructification；
(6) flower bud
After ridge-up bed is well placed, removal bacterium bag upper end without cotton lid and lantern ring, prick sack with rope or rubber band pine, while cutting off above
Extra bag film, control temperature of shed, relative air humidity, intensity of illumination, the CO in environment2Concentration, manages 10d, and sack is long
Button out；
(7) it harvests
It is Harvesting Date when cap is open and flat, spore not yet launches, generally 10d is harvested after buddingging, the first damp fructification
Stop the work of water sprinkling for better material moisture into canopy after harvesting, in 3~5d, pour after mycelia restores into ridge-up bed one time it is permeable, after 10d, form two
Damp button.
2. the method for fermentation material sack soil covering culture vulcanized ester as described in claim 1, it is characterised in that: the step (3)
Middle normal-pressure sterilization operation is that preceding 4h makes material bag temperature reach 80 DEG C or more, is continuously heating to 100 DEG C later, when material bag temperature is stablized
At 100 DEG C, 10~12h is kept to complete sterilizing.
3. the method for fermentation material sack soil covering culture vulcanized ester as described in claim 1, it is characterised in that: the step (4)
Middle condition of culture are as follows: preceding 10d, room temperature is controlled at 24 DEG C~26 DEG C, and after 10d, room temperature is controlled at 22~24 DEG C.
4. the method for fermentation material sack soil covering culture vulcanized ester as described in claim 1, it is characterised in that: the step (6)
Middle temperature of shed is 14~22 DEG C, and relative air humidity is 85%~95%, and intensity of illumination is 400~600l ux, in environment
CO2Concentration < 0.1%.
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| CN201811398234.0A CN109348986A (en) | 2018-11-22 | 2018-11-22 | A kind of method of fermentation material sack soil covering culture vulcanized ester |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110972806A (en) * | 2019-11-22 | 2020-04-10 | 安康市农业科学研究院 | Cultivation method and artificial cultivation method of sulphur vermilion strain |
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Application publication date: 20190219 |