CN109329077A - A kind of anti parasitic experimental animal padding and preparation method thereof - Google Patents
A kind of anti parasitic experimental animal padding and preparation method thereof Download PDFInfo
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- CN109329077A CN109329077A CN201811132810.7A CN201811132810A CN109329077A CN 109329077 A CN109329077 A CN 109329077A CN 201811132810 A CN201811132810 A CN 201811132810A CN 109329077 A CN109329077 A CN 109329077A
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- 238000010171 animal model Methods 0.000 title claims abstract description 64
- 230000002141 anti-parasite Effects 0.000 title claims abstract description 58
- 239000003096 antiparasitic agent Substances 0.000 title claims abstract description 58
- 238000002360 preparation method Methods 0.000 title claims abstract description 34
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 64
- 239000008155 medical solution Substances 0.000 claims abstract description 57
- AZSNMRSAGSSBNP-UHFFFAOYSA-N 22,23-dihydroavermectin B1a Natural products C1CC(C)C(C(C)CC)OC21OC(CC=C(C)C(OC1OC(C)C(OC3OC(C)C(O)C(OC)C3)C(OC)C1)C(C)C=CC=C1C3(C(C(=O)O4)C=C(C)C(O)C3OC1)O)CC4C2 AZSNMRSAGSSBNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- SPBDXSGPUHCETR-JFUDTMANSA-N 8883yp2r6d Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O[C@@H]([C@@H](C)CC4)C(C)C)O3)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1C[C@H](C)[C@@H]([C@@H](C)CC)O[C@@]21O[C@H](C\C=C(C)\[C@@H](O[C@@H]1O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C1)[C@@H](C)\C=C\C=C/1[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\1)O)C[C@H]4C2 SPBDXSGPUHCETR-JFUDTMANSA-N 0.000 claims abstract description 27
- 229960002418 ivermectin Drugs 0.000 claims abstract description 27
- 239000002023 wood Substances 0.000 claims abstract description 24
- HXHWSAZORRCQMX-UHFFFAOYSA-N albendazole Chemical compound CCCSC1=CC=C2NC(NC(=O)OC)=NC2=C1 HXHWSAZORRCQMX-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229960002669 albendazole Drugs 0.000 claims abstract description 22
- 238000001035 drying Methods 0.000 claims abstract description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000002904 solvent Substances 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 239000003643 water by type Substances 0.000 claims abstract description 4
- 235000009051 Ambrosia paniculata var. peruviana Nutrition 0.000 claims description 22
- 235000003097 Artemisia absinthium Nutrition 0.000 claims description 22
- 240000001851 Artemisia dracunculus Species 0.000 claims description 22
- 235000017731 Artemisia dracunculus ssp. dracunculus Nutrition 0.000 claims description 22
- 235000003261 Artemisia vulgaris Nutrition 0.000 claims description 22
- 239000001138 artemisia absinthium Substances 0.000 claims description 22
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims description 22
- NAHTXVIXCMUDLF-DUJPAFNYSA-N Toosendanin Natural products O=C(O[C@H]1C(=O)[C@H]2[C@@](C)([C@H](O)C[C@H]3[C@@]4(C)[C@H](O)OC[C@@]23[C@@H](O)C[C@H]4OC(=O)C)[C@@]23[C@]1(C)[C@H](c1cocc1)C[C@H]2O3)C NAHTXVIXCMUDLF-DUJPAFNYSA-N 0.000 claims description 16
- NAHTXVIXCMUDLF-RFNFAWMESA-N toosendanin Chemical compound C=1([C@H]2[C@]3(C)[C@@H](OC(C)=O)C(=O)[C@H]4[C@@]([C@@]53O[C@@H]5C2)(C)[C@H](O)C[C@H]2[C@@]3(C)[C@H](O)OC[C@]24[C@@H](O)C[C@H]3OC(=O)C)C=COC=1 NAHTXVIXCMUDLF-RFNFAWMESA-N 0.000 claims description 16
- 239000008488 toosendanin Substances 0.000 claims description 16
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 5
- ZWIPGARCRSFMHK-UHFFFAOYSA-N benzene;1h-imidazole Chemical class C1=CNC=N1.C1=CC=CC=C1 ZWIPGARCRSFMHK-UHFFFAOYSA-N 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 5
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- 239000000203 mixture Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 6
- 108010034145 Helminth Proteins Proteins 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 244000000013 helminth Species 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 3
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 3
- 241000238421 Arthropoda Species 0.000 description 2
- 241000242722 Cestoda Species 0.000 description 2
- 241000244206 Nematoda Species 0.000 description 2
- 241000935974 Paralichthys dentatus Species 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 244000079386 endoparasite Species 0.000 description 2
- 229960003692 gamma aminobutyric acid Drugs 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- 231100001231 less toxic Toxicity 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 description 1
- GQXDJKKLSSXFMP-UHFFFAOYSA-N 1,3-bis(hydroxymethyl)benzimidazole-2-thione Chemical compound C1=CC=C2N(CO)C(=S)N(CO)C2=C1 GQXDJKKLSSXFMP-UHFFFAOYSA-N 0.000 description 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 102000004310 Ion Channels Human genes 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229940086181 albendazole 200 mg Drugs 0.000 description 1
- 230000000507 anthelmentic effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940125687 antiparasitic agent Drugs 0.000 description 1
- 150000003851 azoles Chemical class 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 150000002460 imidazoles Chemical class 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000003120 macrolide antibiotic agent Substances 0.000 description 1
- 229940041033 macrolides Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0155—Litter comprising organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to the experimental animal padding fields of biological medicine, provide a kind of anti parasitic experimental animal padding and preparation method thereof, a kind of preparation method of anti parasitic experimental animal padding, it include: to be placed in pest-resistant admixing medical solutions in low temperature drying case after evenly mixing by 0.9~1.1L:10kg with basic padding to dry, basic padding includes wood shavings, solute includes ivermectin and Albendazole in pest-resistant admixing medical solutions, solvent is that mass concentration is 40~70% ethanol waters, the concentration of ivermectin is 10~15mg/L in pest-resistant admixing medical solutions, the concentration of Albendazole is 100~200mg/L in pest-resistant admixing medical solutions.Anti parasitic experimental animal padding made from this method has preferable anti parasitic effect.
Description
Technical field
The present invention relates to the experimental animal padding fields of biological medicine, test in particular to a kind of anti parasitic dynamic
Object padding and preparation method thereof.
Background technique
Currently, padding used in experimental animal is usually the wood shavings after common sterilizing.And animal for research generally requires to move
Object reaches sterile no helminth and is just suitable for testing, since the fault of feeding environment barrier may will lead to experimental animal sense
Contaminate helminth.
Given this special to propose the application.
Summary of the invention
The present invention provides a kind of anti parasitic experimental animal paddings, it is intended to improve padding used in existing experimental animal
The problem of effect without anti parasitic.
The present invention also provides a kind of preparation methods of anti parasitic experimental animal padding, it is intended to prepare with anti-parasitism
The experimental animal padding of worm.
The present invention is implemented as follows:
A kind of preparation method of anti parasitic experimental animal padding, comprising:
Pest-resistant admixing medical solutions are placed in low temperature drying case with basic padding by 0.9~1.1L:10kg after evenly mixing and are dried
Dry, basic padding includes at least one of wood shavings and corncob, and solute includes ivermectin and rosickyite benzene in pest-resistant admixing medical solutions
Imidazoles, solvent are that mass concentration is 40~70% ethanol waters, in pest-resistant admixing medical solutions the concentration of ivermectin be 10~
15mg/L, the concentration of Albendazole is 100~200mg/L in pest-resistant admixing medical solutions.
Further, in preferred embodiments of the present invention, in pest-resistant admixing medical solutions the concentration of ivermectin be 13~
15mg/L, the concentration of Albendazole is 130~180mg/L in pest-resistant admixing medical solutions.
Further, in preferred embodiments of the present invention, the solvent of pest-resistant admixing medical solutions is mass concentration 45~55%
Ethanol water.
It further, further include toosendanin in the solute of pest-resistant admixing medical solutions in preferred embodiments of the present invention.
Further, in preferred embodiments of the present invention, the concentration of toosendanin is 0.5~2mg/ in pest-resistant admixing medical solutions
L。
Further, in preferred embodiments of the present invention, pest-resistant admixing medical solutions are uniformly mixed with basic padding be by
Pest-resistant admixing medical solutions are sprayed on the surface of basic padding.
Further, in preferred embodiments of the present invention, temperature when drying in low temperature drying case is 45~55 DEG C,
Drying time is 5~10min.
Further, in preferred embodiments of the present invention, basic padding further includes wormwood leaf.
Further, in preferred embodiments of the present invention, wormwood leaf in basic padding mass percentage be 5~
15%.
A kind of anti parasitic experimental animal padding, using the preparation method system of above-mentioned anti parasitic experimental animal padding
?.
The beneficial effects of the present invention are: the preparation for the anti parasitic experimental animal padding that the present invention obtains by above-mentioned design
Method, due to being sprayed using the pest-resistant admixing medical solutions of the ivermectin containing appropriate concentration and Albendazole in basic padding
Basic padding is dried to obtain anti parasitic experimental animal padding by surface under appropriate operating condition.Therefore, anti parasitic is real
It tests animal litter and preferable resistance or killing effect is all had for the endoparasite of experimental animal and vermin, so that
Upgrowth situation using the experimental animal of the padding is more preferable, then experimental animal is for that can make experimental result more acurrate when testing.
The anti parasitic experimental animal padding that the present invention obtains by above-mentioned design, due to by the above method be made thus its
Has the effect of preferable anti parasitic.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
A kind of anti parasitic experimental animal padding and preparation method thereof is provided to the embodiment of the present invention below to carry out specifically
It is bright.
A kind of preparation method of anti parasitic experimental animal padding, comprising:
Pest-resistant admixing medical solutions are placed in low temperature drying case with basic padding by 0.9~1.1L:10kg after evenly mixing and are dried
Dry, basic padding includes wood shavings, and solute includes ivermectin and Albendazole in pest-resistant admixing medical solutions, and solvent is mass concentration
For 40~70% ethanol waters, the concentration of ivermectin is 10~15mg/L in pest-resistant admixing medical solutions, in pest-resistant admixing medical solutions
The concentration of Albendazole is 100~200mg/L.
Ivermectin is novel wide spectrum, efficient, less toxic antibiotics antiparasitic agent, is especially to internal epizoa
Nematode and arthropod, which have, well kills effect, but invalid to tapeworm, fluke and protozoan.It is anti-that it belongs to macrolides
Worm medicine kills effect to nematode and arthropod, is to increase releasing for inhibitory transmitter γ-aminobutyric acid (GABA) of polypide
It puts, and opens the Cl ion channel of glutamic acid control, enhance neu to the permeability of Cl, thus the biography of block nerves signal
It passs, final neural paralysis makes muscle cell lose contractility, so as to cause polypide death.
Albendazole: being the most popular wide spectrum of domestic animal doctor, novel anthelmintic efficiently, less toxic, to animal line
Worm, fluke, tapeworm have expeling effect, inhibit absorption of the polypide to glucose, cause polypide gradually dead due to energy exhaustion
It dies, expelling parasite can be carried out to internal and external.Quality is stated more than ivermectin and Albendazole and basic padding to carry out
After mixing, ivermectin and the simultaneously mutual booster action of thibenzazoline make up the deficiency being individually present, can reach in good antibody
The effect of vermin.
Further, to make the insect resistant effect of pest-resistant admixing medical solutions more preferable, ivermectin is dense in pest-resistant admixing medical solutions
Degree is 13~15mg/L, and the concentration of Albendazole is 130~180mg/L in pest-resistant admixing medical solutions.It further, is guarantee
Ivermectin and Albendazole are dissolved completely in ethyl alcohol and do not make the dosage of ethyl alcohol excessively cause to waste again, pest-resistant mixing medicine
The solvent of liquid is 45~55% ethanol water of mass concentration.
Further, pest-resistant admixing medical solutions further include toosendanin.Toosendanin can promote the decomposition generation of polypide atriphos
It thanks to quickening, causes energy supply insufficient and polypide substantially reduces the resistant function of ivermectin and Albendazole,
And make the pest-resistant insecticidal effect of pest-resistant admixing medical solutions more preferable.Further, in pest-resistant admixing medical solutions toosendanin concentration be 0.5~
2mg/L。
Basic padding includes wood shavings, and wood shavings can be wood shavings made of any nontoxic timber.Further, basic padding is also
Including wormwood leaf.Wormwood leaf has certain antibacterial insecticidal action, and nontoxic, can inhibit anti parasitic real to a certain extent
Test the growth of microorganism in animal litter.
Further, in order to not influence the lofting effect of wood shavings, and bacteriostasis is preferable, wormwood leaf matter in basic padding
Measuring percentage composition is 5~15%.
Specifically, prepare the wood shavings after drying and being sterilized and wormwood first, the quality of wood shavings and wormwood as needed is mixed
It closes and basic padding is uniformly made.Then, according to pest-resistant admixing medical solutions to be used amount, weigh ivermectin, rosickyite benzene miaow
Azoles, toosendanin and ethyl alcohol will weigh obtained above-mentioned raw materials and carries out being mixed to get pest-resistant admixing medical solutions.It will be obtained pest-resistant mixed
The surface that medical fluid is uniformly sprayed on basic padding is closed, after sprayed, basic padding is transferred in low temperature drying case and is dried, is
Guaranteeing drying quickly and completely, and does not damage the drug effect of effective component in pest-resistant admixing medical solutions, temperature when drying is 45~
55 DEG C, drying time is that 5~10min obtains anti parasitic experimental animal padding.
A kind of anti parasitic experimental animal padding provided by the invention is specifically described below in conjunction with specific embodiment.
Embodiment 1
Present embodiments provide a kind of anti parasitic experimental animal padding and preparation method thereof.
A kind of anti parasitic experimental animal padding, comprising:
Wood shavings and wormwood after preparation is dry and sterilized, wood shavings and wormwood 95:5 in mass ratio are uniformly mixed and are made
The basis 10kg padding.
Ivermectin 10mg, Albendazole 200mg and toosendanin 0.5mg are weighed, above-mentioned tablet is dissolved in 0.9L matter
Pest-resistant admixing medical solutions are made in the ethanol water that amount percentage composition is 40%.
The pest-resistant admixing medical solutions of 0.9L are uniformly sprayed to the surface of the basis 10kg padding.
The basic padding for being sprayed with pest-resistant admixing medical solutions is transferred in low temperature drying case, under conditions of temperature is 45 DEG C
10min is dried, anti parasitic experimental animal padding is obtained.
Embodiment 2
Present embodiments provide a kind of anti parasitic experimental animal padding and preparation method thereof.
A kind of anti parasitic experimental animal padding, comprising:
Wood shavings and wormwood 85:15 in mass ratio are uniformly mixed system by corncob and wormwood after preparation is dry and sterilized
Obtain the basis 10kg padding.
Ivermectin 15mg, Albendazole 100mg and toosendanin 2mg are weighed, above-mentioned tablet is dissolved in 1.1L mass
Pest-resistant admixing medical solutions are made in the ethanol water that percentage composition is 70%.
The pest-resistant admixing medical solutions of 1.1L are uniformly sprayed to the surface of the basis 10kg padding.
The basic padding for being sprayed with pest-resistant admixing medical solutions is transferred in low temperature drying case, under conditions of temperature is 55 DEG C
5min is dried, anti parasitic experimental animal padding is obtained.
Embodiment 3
Present embodiments provide a kind of anti parasitic experimental animal padding and preparation method thereof.
A kind of anti parasitic experimental animal padding, comprising:
Wood shavings, corncob and wormwood after preparation is dry and sterilized mix wood shavings and wormwood 90:10 in mass ratio equal
The even obtained basis 10kg padding.
Ivermectin 12mg, Albendazole 150mg and toosendanin 1mg are weighed, above-mentioned tablet is dissolved in 1L mass hundred
Pest-resistant admixing medical solutions are made in the ethanol water that point content is 50%.
The pest-resistant admixing medical solutions of 1L are uniformly sprayed to the surface of the basis 10kg padding.
The basic padding for being sprayed with pest-resistant admixing medical solutions is transferred in low temperature drying case, under conditions of temperature is 50 DEG C
7min is dried, anti parasitic experimental animal padding is obtained.
Embodiment 4
Present embodiments provide a kind of anti parasitic experimental animal padding and preparation method thereof.
A kind of anti parasitic experimental animal padding, comprising:
Wood shavings and wormwood after preparation is dry and sterilized, wood shavings and wormwood 92:8 in mass ratio are uniformly mixed and are made
The basis 10kg padding.
Ivermectin 13mg, Albendazole 120mg and toosendanin 0.8mg are weighed, above-mentioned tablet is dissolved in 1L mass
Pest-resistant admixing medical solutions are made in the ethanol water that percentage composition is 45%.
The pest-resistant admixing medical solutions of 1L are uniformly sprayed to the surface of the basis 10kg padding.
The basic padding for being sprayed with pest-resistant admixing medical solutions is transferred in low temperature drying case, under conditions of temperature is 48 DEG C
8min is dried, anti parasitic experimental animal padding is obtained.
Embodiment 5
Present embodiments provide a kind of anti parasitic experimental animal padding and preparation method thereof.
A kind of anti parasitic experimental animal padding, comprising:
Wood shavings and wormwood after preparation is dry and sterilized, wood shavings and wormwood 87:13 in mass ratio are uniformly mixed and are made
The basis 10kg padding.
Ivermectin 14mg, Albendazole 180mg and toosendanin 1.5mg are weighed, above-mentioned tablet is dissolved in 1L mass
Pest-resistant admixing medical solutions are made in the ethanol water that percentage composition is 55%.
The pest-resistant admixing medical solutions of 1L are uniformly sprayed to the surface of the basis 10kg padding.
The basic padding for being sprayed with pest-resistant admixing medical solutions is transferred in low temperature drying case, under conditions of temperature is 53 DEG C
9min is dried, anti parasitic experimental animal padding is obtained.
Embodiment 6
Present embodiments provide a kind of anti parasitic experimental animal padding and preparation method thereof.
A kind of anti parasitic experimental animal padding, comprising:
Wood shavings and wormwood after preparation is dry and sterilized, wood shavings and wormwood 86:14 in mass ratio are uniformly mixed and are made
The basis 10kg padding.
Ivermectin 14mg, Albendazole 160mg and toosendanin 1.8mg are weighed, above-mentioned tablet is dissolved in 1L mass
Pest-resistant admixing medical solutions are made in the ethanol water that percentage composition is 55%.
The pest-resistant admixing medical solutions of 1L are uniformly sprayed to the surface of the basis 10kg padding.
The basic padding for being sprayed with pest-resistant admixing medical solutions is transferred in low temperature drying case, under conditions of temperature is 53 DEG C
8min is dried, anti parasitic experimental animal padding is obtained.
Comparative example 1
This comparative example is substantially the same manner as Example 1, the difference is that the concentration of ivermectin is 6mg/L.
Comparative example 2
This comparative example is substantially the same manner as Example 2, the difference is that the concentration of Albendazole is 150mg/L.
Comparative example 3
This comparative example is substantially the same manner as Example 3, the difference is that basic padding only includes wood shavings.
Comparative example 4
This comparative example is substantially the same manner as Example 3, the difference is that being free of toosendanin in pest-resistant admixing medical solutions.
Comparative example 5
The padding of the experimental animal of this comparative example is the wood shavings after sterilization and drying.
Experimental example
This experimental example is by taking vermin as an example.The good adult flea of 220 upgrowth situations is taken, is divided into 11 groups.
It is No. 1-11 that the padding of the anti parasitic experimental animal padding of 500g embodiment 1-6 and comparative example 1-5 is numbered respectively, is placed in body
Product is 16dm2In draughty transparent vessel, it is dead that every group of flea is observed after 1 day, 2 days, 5 days, 10 days and 30 days
Number is died, and is recorded into table 1.
Dead number of the 1 each group flea of table in alloted days
| 1 day | 2 days | 5 days | 10 days | 30 days | |
| No. 1 | 1 | 8 | 11 | 0 | 0 |
| No. 2 | 0 | 7 | 13 | 0 | 0 |
| No. 3 | 6 | 11 | 3 | 0 | 0 |
| No. 4 | 4 | 10 | 6 | 0 | 0 |
| No. 5 | 4 | 11 | 5 | 0 | 0 |
| No. 6 | 5 | 10 | 5 | 0 | 0 |
| No. 7 | 0 | 4 | 10 | 6 | 0 |
| No. 8 | 0 | 5 | 11 | 4 | 0 |
| No. 9 | 3 | 10 | 7 | 0 | 0 |
| No. 10 | 3 | 11 | 6 | 0 | 0 |
| No. 11 | 0 | 0 | 0 | 2 | 5 |
By table 1, it can be seen that the flea of number 1-6, number 9 and number 10 whole death rays after 5 days, comparison number
There is still the survivals of most of flea after 11 groups, one month., it can be seen that anti parasitic experimental animal padding tool provided by the invention
There is the effect of good anti parasitic.
7 groups of number are compared with 1 group of number, higher than 1 group of number, thus the flea that 7 groups of number obviously survives number of days
As it can be seen that being lower when the concentration of ivermectin is lower than the present invention claims in the range of for the resistance of helminth.By 8 groups of label with
2 groups of number compare, and the flea that 8 groups of number obviously survives number of days higher than 2 groups of number, it can be seen that, Albendazole it is dense
When degree is lower than the present invention claims in the range of, it is lower for the resistance of helminth.
Compare 9 groups of number and 3 groups of number, it can be seen that the flea that 3 groups of number dead number two days later obviously mostly with
9 groups of number, it can be seen that, wormwood leaf is mixed in basic padding, can be improved insect resistant effect.10 groups and 3 groups of number of number are compared,
It can be seen that 3 groups of number of flea dead number two days later obviously mostly with 10 groups of number, it can be seen that, pest-resistant admixing medical solutions
Middle addition toosendanin, can be improved insect resistant effect.
In conclusion the preparation method of anti parasitic experimental animal padding provided by the invention, due to using containing appropriate
The ivermectin of concentration and the pest-resistant admixing medical solutions of Albendazole spray on the surface of basic padding, by basic padding appropriate
Operating condition under be dried to obtain anti parasitic experimental animal padding.Therefore, anti parasitic experimental animal padding is dynamic for testing
The endoparasite of object and vermin all have preferable resistance or killing effect, so that using the experimental animal of the padding
Upgrowth situation it is more preferable, then experimental animal for test when, experimental result can be made more acurrate.
Anti parasitic experimental animal padding provided by the invention, due to passing through the obtained event of the above method, it has preferable resist
The effect of helminth.
The foregoing is merely the preferred embodiment of the present invention, are not intended to restrict the invention, for this field
For technical staff, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any
Modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of preparation method of anti parasitic experimental animal padding characterized by comprising
Pest-resistant admixing medical solutions are placed in low temperature drying case with basic padding by 0.9~1.1L:10kg after evenly mixing and are dried, institute
Stating basic padding includes at least one of wood shavings and corncob, and solute includes ivermectin and rosickyite in the pest-resistant admixing medical solutions
Benzene imidazoles, solvent are that mass concentration is 40~70% ethanol waters, ivermectin described in the pest-resistant admixing medical solutions it is dense
Degree is 10~15mg/L, and the concentration of Albendazole described in the pest-resistant admixing medical solutions is 100~200mg/L.
2. the preparation method of anti parasitic experimental animal padding according to claim 1, which is characterized in that described pest-resistant mixed
The concentration for closing ivermectin described in medical fluid is 13~15mg/L, the concentration of Albendazole described in the pest-resistant admixing medical solutions
For 130~180mg/L.
3. the preparation method of anti parasitic experimental animal padding according to claim 2, which is characterized in that described pest-resistant mixed
The solvent for closing medical fluid is 45~55% ethanol water of mass concentration.
4. the preparation method of anti parasitic experimental animal padding according to claim 1, which is characterized in that described pest-resistant mixed
Closing in the solute of medical fluid further includes toosendanin.
5. the preparation method of anti parasitic experimental animal padding according to claim 4, which is characterized in that described pest-resistant mixed
The concentration for closing toosendanin described in medical fluid is 0.5~2mg/L.
6. the preparation method of anti parasitic experimental animal padding according to claim 1, which is characterized in that will be described pest-resistant
It is the surface that the pest-resistant admixing medical solutions are sprayed on to the basic padding that admixing medical solutions are uniformly mixed with the basic padding.
7. the preparation method of anti parasitic experimental animal padding according to claim 1, which is characterized in that in the low temperature
Temperature when drying in drying box is 45~55 DEG C, and drying time is 5~10min.
8. the preparation method of anti parasitic experimental animal padding according to claim 1, which is characterized in that the base mat
Material further includes wormwood leaf.
9. the preparation method of anti parasitic experimental animal padding according to claim 8, which is characterized in that the wormwood leaf
Mass percentage is 5~15% in the basic padding.
10. a kind of anti parasitic experimental animal padding, which is characterized in that anti-posted using as claim 1-9 is described in any item
The preparation method of infested experimental animal padding is made.
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| CN201811132810.7A CN109329077A (en) | 2018-09-27 | 2018-09-27 | A kind of anti parasitic experimental animal padding and preparation method thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1214909A (en) * | 1998-05-14 | 1999-04-28 | 王玉万 | Complex anti-parasite medicine for animal use |
| CN1318406A (en) * | 2001-01-17 | 2001-10-24 | 云南省农业科学院生物技术研究所 | Veterinary liniment containing azedarachin and its preparation |
| CN1867341A (en) * | 2003-10-17 | 2006-11-22 | 阿克佐诺贝尔公司 | Compositions for controlling parasites comprising a combination of abamectin and milbemycin |
| CN105981652A (en) * | 2015-03-06 | 2016-10-05 | 江苏省协同医药生物工程有限责任公司 | Experimental animal particle litter added with Chinese herbal medicine and preparation method of experimental animal particle litter |
-
2018
- 2018-09-27 CN CN201811132810.7A patent/CN109329077A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1214909A (en) * | 1998-05-14 | 1999-04-28 | 王玉万 | Complex anti-parasite medicine for animal use |
| CN1318406A (en) * | 2001-01-17 | 2001-10-24 | 云南省农业科学院生物技术研究所 | Veterinary liniment containing azedarachin and its preparation |
| CN1867341A (en) * | 2003-10-17 | 2006-11-22 | 阿克佐诺贝尔公司 | Compositions for controlling parasites comprising a combination of abamectin and milbemycin |
| CN105981652A (en) * | 2015-03-06 | 2016-10-05 | 江苏省协同医药生物工程有限责任公司 | Experimental animal particle litter added with Chinese herbal medicine and preparation method of experimental animal particle litter |
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Application publication date: 20190215 |