CN109321553A - A kind of industrial complex enzyme detersive of high temperature resistant cane sugar manufacture and its preparation and application - Google Patents

A kind of industrial complex enzyme detersive of high temperature resistant cane sugar manufacture and its preparation and application Download PDF

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Publication number
CN109321553A
CN109321553A CN201710637515.6A CN201710637515A CN109321553A CN 109321553 A CN109321553 A CN 109321553A CN 201710637515 A CN201710637515 A CN 201710637515A CN 109321553 A CN109321553 A CN 109321553A
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Prior art keywords
resisting
high temperature
detersive
heat
complex enzyme
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林以宋
卢若伟
罗凤玉
韦红桥
骆荣贞
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Guangxi Nongken Sugar Group Golden Sugar Co Ltd
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Guangxi Nongken Sugar Group Golden Sugar Co Ltd
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    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
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    • C12N9/2405—Glucanases
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Abstract

The invention discloses a kind of industrial complex enzyme detersive of high temperature resistant cane sugar manufacture and its preparations and application, belong to the technical field of sugar industry clarification process.The complex enzyme detersive includes heat-resisting dextranase 20~50%, Thermostable α-Amylase 5~35%, heat-resisting pectase 5~25%, high temperature resistant laccase 4~20%, heat-resisting horseradish peroxidase 3~20%, high temperature-resisting cellulase 3~20%, water 0~10% by weight.After above-mentioned raw materials enzyme is mixed according to the ratio, by film filtering, quantitative filling, become product.During primary heating is to reheating, complex enzyme detersive is added in crude sugar juices and carries out clarifying treatment.The present invention suitably matches the complex enzyme formulation that combination is formed, and avoids and only uses single enzyme usually and can not effectively achieve the expected fining efficacy that cleans, no setting is required buffer stage, and it is wide in range to be added site, is limited by temperature smaller.

Description

A kind of industrial complex enzyme detersive of high temperature resistant cane sugar manufacture and its preparation and application
Technical field
The invention belongs to the technical fields of sugar industry clarification process, and in particular to a kind of high temperature resistant cane sugar manufacture is industrial Complex enzyme detersive and its preparation and application.
Background technique
Domestic common sugar refining technology defecation method mainly passes through several approach: 1. be added lime, phosphoric acid, PAM flocculant, SO2 or CO2The precipitation of equal chemical reagent；2. with the suction-operated of active carbon；3 use ion exchange.These routines Defecation method can not effectively remove influence caused by this kind of high viscosity polysaccharide component in sugaring material sugarcane.
Have the research that complex enzyme formulation is used for Closed Circulation in Sugar Production at present, by biological enzymatic catalysis, will be brought in raw material Biological hydrolysis and biological oxidation are carried out to sugaring harmful macromolecules and colloidal substance with newly generated in production process, make macromolecular Become small molecule, nuisance becomes harmless object, and colloidal solution becomes true solution.Syrup viscosity is effectively reduced, accelerates clarification sedimentation Filtering and Sucrose crystallization rate are remarkably improved commerieal sugar yield and product quality, reduce energy consumption.
Chinese patent [publication number] CN102911924A discloses a kind of sugar industry complex enzyme detersive and its preparation Methods and applications；By weight, which includes a- dextranase 35~60%, pectase 20~30%, amylase 10 ~35%, cellulase 5~10% and water 0~15%.Complex enzyme detersive is added in sugar material extraction of the juice workshop section, dosage 10 ~30mg/kg syrup.But the patent complex enzyme operative temperature can only be at 60 DEG C hereinafter, the site being added can only select squeeze juice extracting Or exudation extraction of the juice, while the complex enzyme is limited by temperature, it is also necessary in sugar material extraction of the juice to reheating (100~104 DEG C) rank Duan Jiayi baffle-box extends the reaction time.
Summary of the invention
The problems such as present invention is directed to enzyme non-refractory in enzyme process clarification process of sugar production, and the site of addition is narrow provides a kind of resistance to The industrial complex enzyme detersive of high temperature cane sugar manufacture and its preparation and application, are achieved especially by following technical proposals:
1. a kind of industrial complex enzyme detersive of high temperature resistant cane sugar manufacture, the detersive includes by weight heat-resisting Dextranase 20~50%, Thermostable α-Amylase 5~35%, heat-resisting pectase 5~25%, high temperature resistant laccase 4~20%, Heat-resisting horseradish peroxidase 3~20%, high temperature-resisting cellulase 3~20%, water 0~10%.
Preferably, the detersive includes heat-resisting dextranase 30~40%, high temperature resistant alphalise starch by weight Enzyme 10~30%, heat-resisting pectase 10~20%, high temperature resistant laccase 5~15% are heat-resisting horseradish peroxidase 5~15%, resistance to High temperature fiber element enzyme 5~15%.
Preferably, the detersive includes heat-resisting dextranase 35%, Thermostable α-Amylase by weight 20%, heat-resisting pectase 15%, high temperature resistant laccase 10%, heat-resisting horseradish peroxidase 10%, high temperature-resisting cellulase 10%.
2. a kind of preparation method of the industrial complex enzyme detersive of high temperature resistant cane sugar manufacture, includes the following steps:
(1) by dhdps enzyme by following proportion mixing:
By weight, heat-resisting dextranase 20~50%, Thermostable α-Amylase 5~35%, heat-resisting pectase 5~ 25%, high temperature resistant laccase 4~20%, heat-resisting horseradish peroxidase 3~20%, high temperature-resisting cellulase 3~20%, water 0~ 10%；
(2) product is then become by film filtering, quantitative filling.
3. application of the complex enzyme detersive as described above in cane sugar manufacture is peace and quiet:
Phosphoric acid is added in Cane Mixed Juice, adjusts pH to 5.5~8.0 with milk of lime；Primary heating is to 60~75 DEG C, addition The complex enzyme detersive, the dosage of the detersive are 10~20mg/kg syrup, 10~30 points of the enzymolysis time Clock；Stove drying is carried out after enzymatic hydrolysis, intensity of sulfitation is 7~13CC, and milk of lime is added and adjusts neutralization pH value to 7.0, heating is boiled Sedimentation filtration obtains juice afterwards.
Preferably, the primary heating is to 65~70 DEG C, 15~25 minutes enzymolysis time.
Preferably, the primary heating is to 68 DEG C, the 20 minutes enzymolysis time.
The compound enzyme mechanism of the present invention is: using the effect of laccase, the macromolecular substances premature oxidation such as pigment polymerize, It is filtered to remove.The oxygen content in sugarcane juice is gone using hot horseradish peroxidase, prevents Maillard reaction and causes into The appearance of product fried sugar element rise phenomenon.Under high temperature-resisting cellulase effect, in colloidal solution system, cellulose chain is destroyed The desmacyte wall construction that structure and its hemicellulose are constituted, releases the starch granules wherein wrapped up；Meanwhile in sugarcane juice The macromolecular substances such as starch, glucan are degraded to the soluble small molecule such as dextrin by high temperature resistant type dextranase and alpha-amylase Substance, to reduce the Sucrose that molasses are taken away.Pass through the collaboration of high temperature resistant type dextranase and heat-resisting pectase simultaneously Effect is degraded because the presence of pectin and dextran forms " sugarcane meal ", caused by bent screen blinding plug, what syrup was difficult to filter asks Topic.
Therefore complex enzyme of the present invention can by raw band in liquid glucose pectin, anthocyanidin, protein, starch, glucan, Newly generated caramel colorant, maillard reaction product in fiber and hemicellulose, and production process, reduced sugar alkaline decomposition produce The harmful macromolecules such as object and colloidal substance progress biological hydrolysis, redox and biology are compound, so that macromolecular is become small molecule, have Evil object becomes harmless object, and colloidal solution becomes very molten object.Part non-sugar impurities is removed by precipitating, filtering, and part non-saccharide is miscellaneous Matter reduces the adverse effect in sugar crystal process and eventually enters into molasses since molecular conformation is changed.
The beneficial effects of the present invention are:
The expected fining efficacy that cleans, appropriate proportion combination can not be effectively achieved 1. avoiding and only using single enzyme usually The complex enzyme formulation of formation preferably reaches the clear effect of removal of impurities.
2. be effectively improved liquid glucose quality, reduce material viscosity, improve sedimentation and filtering effect, have improve White Sugar Quality and Product yield, the remarkable result for reducing energy consumption.
3. simple process, without improving original technique, no setting is required buffer stage, it is wide in range to be added site, high temperature resistant, by temperature The limitation of degree is smaller.
Specific embodiment
Combined with specific embodiments below, further details of elaboration is made to the present invention.
Embodiment 1
A kind of preparation of the industrial complex enzyme detersive of high temperature resistant cane sugar manufacture: by weight, composition of raw materials is as follows: resistance to Hot dextranase 50%, Thermostable α-Amylase 35%, heat-resisting pectase 5%, high temperature resistant laccase 4%, heat-resisting horseradish peroxide Compound enzyme 3%, high temperature-resisting cellulase 3%.The preparation method comprises the following steps: dhdps enzyme to be passed through to smart membrane filtration respectively, then in proportion into Enter automatic blender compounding, quantitative filling becomes product.
Embodiment 2
A kind of preparation of the industrial complex enzyme detersive of high temperature resistant cane sugar manufacture: by weight, composition of raw materials is as follows: resistance to Hot dextranase 20%, Thermostable α-Amylase 5%, heat-resisting pectase 25%, high temperature resistant laccase 20%, heat-resisting horseradish peroxide Compound enzyme 10%, high temperature-resisting cellulase 10%, water 10%.The preparation method comprises the following steps: dhdps enzyme is passed through to smart membrane filtration respectively, then Enter automatic blender in proportion to compound, quantitative filling becomes product.
Embodiment 3
A kind of preparation of the industrial complex enzyme detersive of high temperature resistant cane sugar manufacture: by weight, composition of raw materials is as follows: resistance to Hot dextranase 30%, Thermostable α-Amylase 10%, heat-resisting pectase 10%, high temperature resistant laccase 10%, heat-resisting horseradish mistake Oxide enzyme 20%, high temperature-resisting cellulase 20%.The preparation method comprises the following steps: dhdps enzyme is passed through micro-filtrate membrane filtration respectively, then by than Example enters automatic blender and compounds, and quantitative filling becomes product.
Embodiment 4
A kind of preparation of the industrial complex enzyme detersive of high temperature resistant cane sugar manufacture: by weight, composition of raw materials is as follows: resistance to Hot dextranase 35%, Thermostable α-Amylase 20%, heat-resisting pectase 20%, high temperature resistant laccase 15%, heat-resisting horseradish mistake Oxide enzyme 5%, high temperature-resisting cellulase 5%.The preparation method comprises the following steps: dhdps enzyme is passed through micro-filtrate membrane filtration respectively, then in proportion It is compounded into automatic blender, quantitative filling becomes product.
Embodiment 5
A kind of preparation of the industrial complex enzyme detersive of high temperature resistant cane sugar manufacture: by weight, composition of raw materials is as follows: resistance to Hot dextranase 40%, Thermostable α-Amylase 30%, heat-resisting pectase 10%, high temperature resistant laccase 5%, heat-resisting horseradish peroxide Compound enzyme 5%, high temperature-resisting cellulase 10%.The preparation method comprises the following steps: dhdps enzyme to be passed through to smart membrane filtration respectively, then in proportion into Enter automatic blender compounding, quantitative filling becomes product.
Embodiment 6
A kind of preparation of the industrial complex enzyme detersive of high temperature resistant cane sugar manufacture: by weight, composition of raw materials is as follows: resistance to Hot dextranase 35%, Thermostable α-Amylase 20%, heat-resisting pectase 15%, high temperature resistant laccase 10%, heat-resisting horseradish mistake Oxide enzyme 10%, high temperature-resisting cellulase 10%.The preparation method comprises the following steps: dhdps enzyme to be passed through to smart membrane filtration respectively, then by than Example enters automatic blender and compounds, and quantitative filling becomes product.
Embodiment 7
Phosphoric acid is added in Cane Mixed Juice, adjusts pH to 8.0 with milk of lime；Primary heating to 75 DEG C, make by addition embodiment 4 The complex enzyme detersive obtained, the dosage of detersive are 20mg/kg syrup, nature mixing are pumped by pipeline, in sugar industry Assembly line in sufficiently react 30 minutes；Stove drying is carried out after enzymatic hydrolysis, intensity of sulfitation 13CC is added milk of lime and adjusts neutralization PH value to 7.0, boil rear sedimentation filtration and obtain juice by heating.Obtained dextran removal rate is 54%, the rate of filtration and heavy Reduction of speed degree improves 65% or so, haze reduction 71%, phenolic material removal rate 73%, percent of decolourization 71%.
Embodiment 8
Phosphoric acid is added in Cane Mixed Juice, adjusts pH to 5.5 with milk of lime；Primary heating to 60 DEG C, make by addition embodiment 5 The complex enzyme detersive obtained, the dosage of detersive are 10mg/kg syrup, nature mixing are pumped by pipeline, in sugar industry Assembly line in sufficiently react 10 minutes；Stove drying is carried out after enzymatic hydrolysis, intensity of sulfitation 7CC is added milk of lime and adjusts neutralization PH value to 7.0, boil rear sedimentation filtration and obtain juice by heating.Obtained dextran removal rate is 46%, the rate of filtration and heavy Reduction of speed degree improves 56% or so, haze reduction 63%, phenolic material removal rate 71%, percent of decolourization 69%.
Embodiment 9
Phosphoric acid is added in Cane Mixed Juice, adjusts pH to 7.0 with milk of lime；Primary heating to 65 DEG C, make by addition embodiment 6 The complex enzyme detersive obtained, the dosage of detersive are 15mg/kg syrup, nature mixing are pumped by pipeline, in sugar industry Assembly line in sufficiently react 15 minutes；Stove drying is carried out after enzymatic hydrolysis, intensity of sulfitation 10CC is added milk of lime and adjusts neutralization PH value to 7.0, boil rear sedimentation filtration and obtain juice by heating.Obtained dextran removal rate is 71%, the rate of filtration and heavy Reduction of speed degree improves 59% or so, haze reduction 73%, phenolic material removal rate 80%, percent of decolourization 79%.
Embodiment 10
Phosphoric acid is added in Cane Mixed Juice, adjusts pH to 6.5 with milk of lime；Primary heating to 70 DEG C, make by addition embodiment 3 The complex enzyme detersive obtained, the dosage of detersive are 12mg/kg syrup, nature mixing are pumped by pipeline, in sugar industry Assembly line in sufficiently react 25 minutes；Stove drying is carried out after enzymatic hydrolysis, intensity of sulfitation 9CC is added milk of lime and adjusts neutralization PH value to 7.0, boil rear sedimentation filtration and obtain juice by heating.Obtained dextran removal rate is 61%, the rate of filtration and heavy Reduction of speed degree improves 63% or so, haze reduction 79%, phenolic material removal rate 82%, percent of decolourization 89%.
Embodiment 11
Phosphoric acid is added in Cane Mixed Juice, adjusts pH to 7.8 with milk of lime；Primary heating to 68 DEG C, make by addition embodiment 4 The complex enzyme detersive obtained, the dosage of detersive are 18mg/kg syrup, nature mixing are pumped by pipeline, in sugar industry Assembly line in sufficiently react 20 minutes；Stove drying is carried out after enzymatic hydrolysis, intensity of sulfitation 12CC is added milk of lime and adjusts neutralization PH value to 7.0, boil rear sedimentation filtration and obtain juice by heating.Obtained dextran removal rate is 61%, the rate of filtration and heavy Reduction of speed degree improves 63% or so, haze reduction 79%, phenolic material removal rate 82%, percent of decolourization 89%.
Although above having used general explanation, specific embodiment and test, the present invention is made to retouch in detail It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art 's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed Range.

Claims (8)

1. a kind of industrial complex enzyme detersive of high temperature resistant cane sugar manufacture, which is characterized in that the detersive includes by weight Heat-resisting dextranase 20~50%, the Thermostable α-Amylase 5~35%, heat-resisting pectase 5~25%, high temperature resistant laccase of meter 4~20%, heat-resisting horseradish peroxidase 3~20%, high temperature-resisting cellulase 3~20%, water 0~10%.
2. complex enzyme detersive as described in claim 1, which is characterized in that the detersive includes by weight heat-resisting Dextranase 30~40%, Thermostable α-Amylase 10~30%, heat-resisting pectase 10~20%, high temperature resistant laccase 5~ 15%, heat-resisting horseradish peroxidase 5~15%, high temperature-resisting cellulase 5~15%.
3. complex enzyme detersive as claimed in claim 2, which is characterized in that the detersive includes by weight heat-resisting Dextranase 35%, Thermostable α-Amylase 20%, heat-resisting pectase 15%, high temperature resistant laccase 10%, heat-resisting horseradish peroxide Compound enzyme 10%, high temperature-resisting cellulase 10%.
4. a kind of preparation method of the industrial complex enzyme detersive of high temperature resistant cane sugar manufacture, which comprises the steps of:
(1) by dhdps enzyme by following proportion mixing:
By weight, heat-resisting dextranase 20~50%, Thermostable α-Amylase 5~35%, heat-resisting pectase 5~ 25%, high temperature resistant laccase 4~20%, heat-resisting horseradish peroxidase 3~20%, high temperature-resisting cellulase 3~20%, water 0~ 10%；
(2) product is then become by film filtering, quantitative filling.
5. application of the complex enzyme detersive in cane sugar manufacture is peace and quiet as described in claims 1 to 3 any claim.
6. application of the complex enzyme detersive as claimed in claim 5 in cane sugar manufacture is peace and quiet, which is characterized in that mixed in sugarcane It closes juice and phosphoric acid is added, adjust pH to 5.5~8.0 with milk of lime；It is peace and quiet to add the complex enzyme to 60~75 DEG C for primary heating Agent, the dosage of the detersive are 10~20mg/kg syrup, the enzymolysis time 10~30 minutes；It is carried out after enzymatic hydrolysis Stove drying, intensity of sulfitation are 7~13CC, and milk of lime is added and adjusts neutralization pH value to 7.0, heating is boiled rear sedimentation filtration and obtained clearly Juice.
7. application of the complex enzyme detersive as claimed in claim 6 in cane sugar manufacture is peace and quiet, which is characterized in that described primary 65~70 DEG C are heated to, 15~25 minutes enzymolysis time.
8. application of the complex enzyme detersive as claimed in claim 7 in cane sugar manufacture is peace and quiet, which is characterized in that described primary 68 DEG C are heated to, the 20 minutes enzymolysis time.
CN201710637515.6A 2017-07-31 2017-07-31 A kind of industrial complex enzyme detersive of high temperature resistant cane sugar manufacture and its preparation and application Pending CN109321553A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110205409A (en) * 2019-06-28 2019-09-06 中粮屯河崇左糖业有限公司 Sulfurous method sugar clarification method
CN110343787A (en) * 2019-06-28 2019-10-18 中粮屯河崇左糖业有限公司 The defecation method of starch during removal sugaring
CN110343788A (en) * 2019-06-28 2019-10-18 中粮屯河崇左糖业有限公司 Lime method sugar clarification method
CN111690779A (en) * 2020-07-24 2020-09-22 广西飞鹏节能环保科技有限公司 Device for removing sucrose starch and acidic flocculate
CN112048496A (en) * 2020-08-31 2020-12-08 南宁雄晋生物科技有限公司 Complex enzyme preparation special for sugar production and preparation method and application thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110205409A (en) * 2019-06-28 2019-09-06 中粮屯河崇左糖业有限公司 Sulfurous method sugar clarification method
CN110343787A (en) * 2019-06-28 2019-10-18 中粮屯河崇左糖业有限公司 The defecation method of starch during removal sugaring
CN110343788A (en) * 2019-06-28 2019-10-18 中粮屯河崇左糖业有限公司 Lime method sugar clarification method
CN111690779A (en) * 2020-07-24 2020-09-22 广西飞鹏节能环保科技有限公司 Device for removing sucrose starch and acidic flocculate
CN112048496A (en) * 2020-08-31 2020-12-08 南宁雄晋生物科技有限公司 Complex enzyme preparation special for sugar production and preparation method and application thereof
CN112048496B (en) * 2020-08-31 2023-03-14 南宁雄晋生物科技有限公司 Complex enzyme preparation special for sugar production and preparation method and application thereof

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