CN109315226A - A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink - Google Patents
A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink Download PDFInfo
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- CN109315226A CN109315226A CN201811318158.8A CN201811318158A CN109315226A CN 109315226 A CN109315226 A CN 109315226A CN 201811318158 A CN201811318158 A CN 201811318158A CN 109315226 A CN109315226 A CN 109315226A
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- mushroom
- microcapsules
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- needle mushroom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Environmental Sciences (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention discloses a kind of preparation methods of needle mushroom mushroom cell microcapsules fermented health drink, microcapsules are made with Flammulina velutipes mycelium cell in mushroom using micro capsule technology first to ferment again, pass through the covering material and concentration of scientific and reasonable selection microcapsules, obtained microcapsules are able to maintain that the activity of cell inside microcapsules, and mushroom and needle mushroom can be subjected to mixed fermentation, change form existing for microorganism, product has good mobility and dispersibility, thallus inactivation can be effectively prevented, improve the stability of microbiology class product, it is carried out in people's body by way of beverage is made, it avoids being influenced by gastric juice, it is absorbed after directly reaching small intestine, play good health-care effect；The present invention is by obtaining finished product through homogeneous, degassing, filling, sterilizing, the transparent clarification of product, and in faint yellow, fragrance and flavour with mushroom, needle mushroom, comfortable acid, good mouthfeel, trophic function is comprehensive, has a vast market foreground.
Description
Technical field
The present invention relates to edible mushroom technical field more particularly to a kind of needle mushroom mushroom cell microcapsules fermented health drinks
Preparation method.
Background technique
Genus flammulina is in Eumycota, Basidiomycotina, Hymenomycetes, Agaricales, Tricholomataceae, money Pseudomonas.Ion Absorption of Flammulina Velutipes contains
The several mineral materials such as calcium, phosphorus, iron, and Zn content is very high, plays the role of promoting children's intelligence development and brain tonic；Protein content is high
And it is high-quality, contain 18 kinds of amino acid, including 8 kinds of amino acid needed by human, especially lysine and arginine content is higher.Rely
Propylhomoserin can promote upgrowth and development of children, and enhancing memory improves intelligence.
Mushroom is under the jurisdiction of Eumycota, Basidiomycetes, Agaricales, Pleurotaceae, Lentinus, is known as the title of " mountain delicacy " in China,
It is not only delicious, and meat is fresh and tender palatable.Studying at present more is lentinan, in addition to this protein in mushroom, mineral member
Plain microelement vitamin and some a small amount of volatile components are also important nutritional ingredient.Studies have shown that often feeding mushroom has
Prevention rickets catches a cold, reduces blood pressure, blood lipid, improve immunity of organisms, is antitumor, adjusting immune function and stimulation interferon
The function of formation.
As the improvement of people's living standards, huge variation has had occurred to the hobby of beverage and demand in consumer, just
Develop towards nutrition, health care direction.People have generally accepted mushroom, Trients of The Flammulina Velutipes And Its Functions at present, fragrant
Mushroom, needle mushroom carry out fermenting and producing beverage in China also it has been reported that if can be placed on both mushroom, needle mushroom same anti-respectively
It answers in system and ferments, be developed into health-care drink, then nutrition, effect more fully, meet consumer to the need of health
It wants.Microencapsulation cell technology is that different cells are fixed on in special carrier a kind of technology for forming microcapsules, is given birth in protection
Object bioactive molecule, tissue and cell achieve preferable effect in terms of adverse environment to fight, be widely used in food, medicine,
The technical fields such as chemical industry.The present invention plans mushroom and Flammulina velutipes mycelium carries out microcapsule coated, carries out while fermenting, then adds
Other compositions are prepared into composite fermented beverage, and product meets modern food nutrient health idea, full-featured, unique flavor.
Summary of the invention
The object of the invention is to remedy the disadvantages of known techniques, provides a kind of needle mushroom mushroom cell microcapsules fermentation
The preparation method of health drink.
The present invention is achieved by the following technical solutions:
A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink, comprising the following specific steps
(1) on ultra-clean station, with the Needle mushroom strain of scissors clip nail length, PDA slant medium is sent to tweezers
In, 27-28 DEG C of culture activates strain sufficiently, mushroom is similarly taken thumbnail, with Needle mushroom strain using same activation
Operation；
(2) it is operated in gnotobasis, chooses the preferable mushroom of growing way, Flammulina velutipes mycelium, carry out mixed bacterium according to a certain percentage,
It is then placed in bead to break up in the sodium alginate colloid that mycelium is added to after cell 5.0%, controls certain bacterium glue ratio,
After mixing well, mixed liquor is dripped into the calcium chloride solution of concentration 2.1%, microcapsules are made, obtained microcapsules are put into not
Synthermal middle stewing process；
(3) culture dish is cleaned up with aseptic deionized water, is inoculated into the microcapsules that step (2) obtains after being blotted with filter paper
In culture medium, pH value, the temperature of culture medium are controlled, is cultivated 11-12 days, by fermentation liquid centrifugal filtration, master of the filtrate as beverage
Material；
(4) filtrate that step (3) obtains is deployed according to formula with stabilizer, citric acid, sucrose, honey, is then placed in
Homogeneous is carried out in homogenizer, filling to beverage progress later, sealing sterilizes filling beverage 10-15 minutes at 121 DEG C,
Being cooled to room temperature can case.
A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink, step are pressed described in (2)
Mixed bacterium is carried out according to certain ratio, refers to and is mixed according to the ratio of mushroom mycelium and Flammulina velutipes mycelium 2:1.
A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink, control described in step (2)
Certain bacterium glue ratio is made, refers to the bacterium glue ratio of control 1:3.
A kind of preparation method of the needle mushroom mushroom cell microcapsules fermented health drink, described in step (2)
To microcapsules be put into stewing process in different temperatures, specific practice are as follows: microcapsules are put into 3-4 DEG C of refrigerator and stand 2 hours,
2 hours are stood in 25 DEG C of constant temperature after taking-up.
A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink, control described in step (3)
PH value, the temperature of culture medium processed, refer to control culture medium pH value be 5.5,24 DEG C of temperature.
A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink, step are matched described in (4)
Side are as follows: microcapsules ferment filtrate 16-18%, stabilizer 1.5-2%, citric acid 0.12-0.15%, sucrose 7-8%, honey 1.5-2%,
Water surplus, wherein stabilizer compares mix products according to the quality of 1:2 for sodium carboxymethylcellulose and xanthan gum.
The invention has the advantages that the present invention uses micro capsule technology that micro- glue is made in mushroom and Flammulina velutipes mycelium cell first
Capsule ferments again, and by the covering material and concentration of scientific and reasonable selection microcapsules, obtained microcapsules are able to maintain that
The activity of cell inside microcapsules, and mushroom and needle mushroom can be subjected to mixed fermentation, change form existing for microorganism,
Product has good mobility and dispersibility, can be effectively prevented thallus inactivation, improve the stability of microbiology class product,
It is carried out by way of beverage is made in people's body, avoids being influenced by gastric juice, absorbed, played good after directly reaching small intestine
Good health-care effect；The present invention is by obtaining finished product, the transparent clarification of product, in faint yellow, tool through homogeneous, degassing, filling, sterilizing
There are the fragrance and flavour of mushroom, needle mushroom, comfortable acid, good mouthfeel, trophic function is comprehensive, meets state health standards, has
There is vast market prospect.
Specific embodiment
A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink, comprising the following specific steps
(1) on ultra-clean station, with the Needle mushroom strain of scissors clip nail length, PDA slant medium is sent to tweezers
In, 27 DEG C of cultures activate strain sufficiently, mushroom are similarly taken thumbnail, are grasped with Needle mushroom strain using same activation
Make；
(2) it is operated in gnotobasis, chooses the preferable mushroom of growing way, Flammulina velutipes mycelium, carry out mixed bacterium according to the ratio of 2:1,
It is then placed in bead to break up in the sodium alginate colloid that mycelium is added to after cell 5.0%, controls the bacterium glue ratio of 1:3, fill
Divide after mixing, mixed liquor is dripped into the calcium chloride solution of concentration 2.1%, microcapsules are made, obtained microcapsules are put into 3 DEG C of ice
2 hours are stood in case, stands 2 hours in 25 DEG C of constant temperature after taking-up；
(3) culture dish is cleaned up with aseptic deionized water, is inoculated into the microcapsules that step (2) obtains after being blotted with filter paper
In culture medium, the pH value for controlling culture medium is 5.5,24 DEG C of temperature, is cultivated 11 days, by fermentation liquid centrifugal filtration, filtrate is as drink
The major ingredient of material；
(4) filtrate that step (3) obtains is deployed according to formula with stabilizer, citric acid, sucrose, honey, is then placed in
Homogeneous is carried out in homogenizer, filling to beverage progress later, sealing sterilizes filling beverage 10 minutes at 121 DEG C, cooling
It can case to room temperature.
A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink, step are matched described in (4)
Side are as follows: microcapsules ferment filtrate 16%, stabilizer 1.5%, citric acid 0.12%, sucrose 7%, honey 1.5%, water surplus, wherein stable
Agent compares mix products according to the quality of 1:2 for sodium carboxymethylcellulose and xanthan gum.
Claims (6)
1. a kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink, which is characterized in that including in detail below
Step:
(1) on ultra-clean station, with the Needle mushroom strain of scissors clip nail length, PDA slant medium is sent to tweezers
In, 27-28 DEG C of culture activates strain sufficiently, mushroom is similarly taken thumbnail, with Needle mushroom strain using same activation
Operation；
(2) it is operated in gnotobasis, chooses the preferable mushroom of growing way, Flammulina velutipes mycelium, carry out mixed bacterium according to a certain percentage,
It is then placed in bead to break up in the sodium alginate colloid that mycelium is added to after cell 5.0%, controls certain bacterium glue ratio,
After mixing well, mixed liquor is dripped into the calcium chloride solution of concentration 2.1%, microcapsules are made, obtained microcapsules are put into not
Synthermal middle stewing process；
(3) culture dish is cleaned up with aseptic deionized water, is inoculated into the microcapsules that step (2) obtains after being blotted with filter paper
In culture medium, pH value, the temperature of culture medium are controlled, is cultivated 11-12 days, by fermentation liquid centrifugal filtration, master of the filtrate as beverage
Material；
(4) filtrate that step (3) obtains is deployed according to formula with stabilizer, citric acid, sucrose, honey, is then placed in
Homogeneous is carried out in homogenizer, filling to beverage progress later, sealing sterilizes filling beverage 10-15 minutes at 121 DEG C,
Being cooled to room temperature can case.
2. a kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink according to claim 1, special
Sign is, carries out mixed bacterium described in step (2) according to a certain percentage, refers to according to mushroom mycelium with Flammulina velutipes mycelium 2:1's
Ratio is mixed.
3. a kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink according to claim 1, special
Sign is, certain bacterium glue ratio is controlled described in step (2), refers to the bacterium glue ratio of control 1:3.
4. a kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink according to claim 1, special
Sign is that the microcapsules obtained described in step (2) are put into stewing process in different temperatures, specific practice are as follows: put microcapsules
Enter in 3-4 DEG C of refrigerator and stand 2 hours, stands 2 hours in 25 DEG C of constant temperature after taking-up.
5. a kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink according to claim 1, special
Sign is, pH value, the temperature of culture medium are controlled described in step (3), refers to that the pH value of control culture medium is 5.5,24 DEG C of temperature.
6. a kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink according to claim 1, special
Sign is, formula described in step (4) are as follows: microcapsules ferment filtrate 16-18%, stabilizer 1.5-2%, citric acid 0.12-
0.15%, sucrose 7-8%, honey 1.5-2%, water surplus, wherein stabilizer is sodium carboxymethylcellulose and xanthan gum according to 1:2's
Quality compares mix products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811318158.8A CN109315226A (en) | 2018-11-07 | 2018-11-07 | A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811318158.8A CN109315226A (en) | 2018-11-07 | 2018-11-07 | A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink |
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| CN109315226A true CN109315226A (en) | 2019-02-12 |
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| CN201811318158.8A Pending CN109315226A (en) | 2018-11-07 | 2018-11-07 | A kind of preparation method of needle mushroom mushroom cell microcapsules fermented health drink |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1146684A (en) * | 1997-08-06 | 1999-02-23 | Nakada Sangyo Kk | Lactobacillary beverage using flammulina velutipes |
| CN1511193A (en) * | 2001-05-07 | 2004-07-07 | ¹��������ʽ���� | Method for preparing lactic acid fermented solution of mushroom and lactic acid fermented solution of mushroom produced thereby |
| CN101164452A (en) * | 2006-10-17 | 2008-04-23 | 天津科技大学 | Double-preparation type composite mushroom functional beverage host and preparation method thereof |
| CN102805335A (en) * | 2012-07-20 | 2012-12-05 | 黄晓青 | Method for preparing edible fungus health-care product |
-
2018
- 2018-11-07 CN CN201811318158.8A patent/CN109315226A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1146684A (en) * | 1997-08-06 | 1999-02-23 | Nakada Sangyo Kk | Lactobacillary beverage using flammulina velutipes |
| CN1511193A (en) * | 2001-05-07 | 2004-07-07 | ¹��������ʽ���� | Method for preparing lactic acid fermented solution of mushroom and lactic acid fermented solution of mushroom produced thereby |
| CN101164452A (en) * | 2006-10-17 | 2008-04-23 | 天津科技大学 | Double-preparation type composite mushroom functional beverage host and preparation method thereof |
| CN102805335A (en) * | 2012-07-20 | 2012-12-05 | 黄晓青 | Method for preparing edible fungus health-care product |
Non-Patent Citations (1)
| Title |
|---|
| 苗敬芝 等: "灵芝、金针菇细胞胶囊化发酵饮料的研制", 《食品工业》 * |
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Application publication date: 20190212 |
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