CN109312379B - 多功能重组核苷酸依赖性糖基转移酶蛋白及其糖基化的方法 - Google Patents

多功能重组核苷酸依赖性糖基转移酶蛋白及其糖基化的方法 Download PDF

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CN109312379B
CN109312379B CN201780031562.4A CN201780031562A CN109312379B CN 109312379 B CN109312379 B CN 109312379B CN 201780031562 A CN201780031562 A CN 201780031562A CN 109312379 B CN109312379 B CN 109312379B
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sugar
glycosylation
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diglycosylated
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CN109312379A (zh
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A·饶
R·纳加尔
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Council of Scientific and Industrial Research CSIR
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

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CN201780031562.4A 2016-04-05 2017-03-30 多功能重组核苷酸依赖性糖基转移酶蛋白及其糖基化的方法 Active CN109312379B (zh)

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IN201611011974 2016-04-05
PCT/IN2017/050116 WO2017175239A1 (en) 2016-04-05 2017-03-30 A multifunctional recombinant nucleotide dependent glycosyltransferase protein and its method of glycosylation thereof
IN201611011974A IN201611011974A (enExample) 2016-04-05 2017-03-30

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US (1) US11485991B2 (enExample)
EP (1) EP3423587B1 (enExample)
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EP3844271A4 (en) * 2018-08-29 2022-06-01 Council of Scientific and Industrial Research Recombinant microbial system for directed evolution of glycocins and a method of preparation thereof
US20220228186A1 (en) * 2019-05-23 2022-07-21 Arzeda Corp. Compositions and methods for producing steviol glycosides

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101151367A (zh) * 2004-02-04 2008-03-26 尼澳斯技术股份有限公司 哺乳动物糖基转移酶的重折叠方法
CN103328630A (zh) * 2011-01-20 2013-09-25 詹尼温生物技术有限责任公司 新型岩藻糖基转移酶及其应用
US9333227B2 (en) * 2013-08-19 2016-05-10 Syngulon Sa. Controlled growth of microorganisms

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NZ258525A (en) 1992-11-27 1997-05-26 Ciba Geigy Ag Proteins with glycosyltransferase activity and their recombinant production
US6127153A (en) 1995-06-07 2000-10-03 Neose Technologies, Inc. Method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and gene encoding a polyglycosyltransferase
EP1066388A2 (de) 1998-03-25 2001-01-10 GVS Gesellschaft für Erwerb und Verwertung Landwirtschaftlicher Pflanzensorten MBH Prozessive glycosyltransferase
EP1115740A1 (en) 1998-09-23 2001-07-18 The Regents of the University of California Synthetic peptides, conjugation reagents and methods
AU2001268885A1 (en) 2000-06-28 2002-01-08 National Research Council Of Canada Helicobacter dd-heptosyltransferase
NZ538439A (en) 2002-07-23 2007-02-23 Neose Technologies Inc Synthesis of oligosaccharides, glycolipids, and glycoproteins using bacterial glycosyltransferases
US8361961B2 (en) 2004-01-08 2013-01-29 Biogenerix Ag O-linked glycosylation of peptides
CA2638779C (en) 2006-01-31 2017-01-03 National Research Council Of Canada Production of polysialic acid containing glyconjugates using a self-priming polysialyltransferase
WO2008011633A2 (en) 2006-07-21 2008-01-24 Neose Technologies, Inc. Glycosylation of peptides via o-linked glycosylation sequences
EP2162535A4 (en) 2007-06-04 2011-02-23 Novo Nordisk As O-linked glycosylation using N-acetylglucosamine transferases
PT2257307T (pt) 2008-02-20 2018-10-22 Glaxosmithkline Biologicals Sa Bioconjugados preparados a partir de proteínas n-glicosiladas recombinantes de células procariotas
FR2954138B1 (fr) 2009-12-17 2012-03-02 Oreal Procede cosmetique de traitement des odeurs corporelles utilisant une composition a base de bacteriocines
EP2664671A4 (en) 2011-01-14 2014-09-10 Suntory Holdings Ltd New Glycosyltransferase and its use
CN102389030B (zh) 2011-09-16 2013-12-25 李叶青 枯草杆菌糖肽素168的用途
EP2790715B1 (en) 2011-12-13 2016-12-07 Centre National De La Recherche Scientifique Modified peptides and their use for treating autoimmune diseases

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101151367A (zh) * 2004-02-04 2008-03-26 尼澳斯技术股份有限公司 哺乳动物糖基转移酶的重折叠方法
CN103328630A (zh) * 2011-01-20 2013-09-25 詹尼温生物技术有限责任公司 新型岩藻糖基转移酶及其应用
US9333227B2 (en) * 2013-08-19 2016-05-10 Syngulon Sa. Controlled growth of microorganisms

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Effects of Glycosylation on the Stability of Protein Pharmaceuticals;RICARDO J.SOLA等;《JOURNAL OF PHARMACEUTICAL SCIENCES》;20090430;第98卷(第4期);全文 *
Enterococcus faecalis V583 plasmid pTEF2, complete sequence;Paulsen I.等;《DATABASE EMBL》;20030329;第32页Sequence部分 *
Features and applications of bacterial glycosyltransferases: current state and prospects;Andriy Luzhetskyy等;《Appl Microbiol Biotechnol》;20080906;第80卷(第6期);全文 *
glycosyltransferase family 2 protein [Enterococcus faecalis];佚名;《GenBank》;20130526;第1页ORIGIN部分 *

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US20210071221A1 (en) 2021-03-11
IN201611011974A (enExample) 2018-02-02
CN109312379A (zh) 2019-02-05
WO2017175239A4 (en) 2017-12-28
US11485991B2 (en) 2022-11-01
WO2017175239A1 (en) 2017-10-12
EP3423587A1 (en) 2019-01-09
EP3423587B1 (en) 2020-06-24

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