CN109295132A - A kind of culture medium and preparation method thereof producing sodium hyaluronate - Google Patents
A kind of culture medium and preparation method thereof producing sodium hyaluronate Download PDFInfo
- Publication number
- CN109295132A CN109295132A CN201811189723.5A CN201811189723A CN109295132A CN 109295132 A CN109295132 A CN 109295132A CN 201811189723 A CN201811189723 A CN 201811189723A CN 109295132 A CN109295132 A CN 109295132A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- parts
- mixed
- preparation
- inorganic salts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention provides a kind of culture mediums and preparation method thereof for producing sodium hyaluronate, the culture medium includes following components by weight: 29-38 parts of white granulated sugar, 7-15 parts of yeast extract, inorganic salts 2.5-4 parts of buffering, 14-28 parts of L-arginine, 10-30 parts of polyether antifoam agent, 2.5-5 parts of wheat bran, 1.5-3 parts of the inorganic salts of microelement, 40-60 parts of water；Culture medium provided by the invention is reasonably combined by the inorganic salts of L-arginine, wheat bran and microelement etc., when using streptococcus zooepidemicus H23 fermenting and producing sodium hyaluronate, greatly improve the yield of sodium hyaluronate, its yield can reach 6.7g/L-7.2g/L, breach the bottleneck of Sodium Hyaluronate industry technology development, production cost is reduced, is had a good application prospect.
Description
Technical field
The invention belongs to field of fermentation engineering, are related to a kind of culture medium and preparation method thereof for producing sodium hyaluronate.
Background technique
Sodium hyaluronate (Sodium Hyaluronate) also known as Sodium Hyaluronate are handed over repeatedly by N- acetyl glucosamine aldehydic acid
A kind of macromolecule polysaccharide body biomaterial for replacing and being formed.Sodium hyaluronate is the main component of knuckle synovia, is cartilage matrix
One of ingredient.Lubricating action is played in articular cavity, can cover and protect articular cartilage, improves the contracture of joint, inhibits cartilage degeneration
Surface variations improve pathologic joint fluid, increase and drip sliding function.The method for obtaining sodium hyaluronate at present is mainly the cunning from animal
Sodium hyaluronate is extracted in liquid, skin, rooster comb and umbilical cord.But this method have the defects that it is certain, for restrict glass
The development that glass acid sodium extracts.Currently, biological fermentation process can make up conventional method, there are problems, are sodium hyaluronate PRODUCTION TRAITSs
Hot Contents.
CN105255969A discloses a kind of method for improving hyaluronic acid volume of production of fermentation production, belongs to fermentation engineering
Field.The method utilize with strain fermentation produce hyaluronic acid, ferment used medium in added with ribose, soybean lecithin one
Kind or two or more mixtures.Hyaluronic acid volume of production can be effectively improved using the method, improved up to 26%.But this side
The yield of hyaluronic acid is up to 6.3g/L in method, it is also necessary to be further increased.
CN107338201A discloses a kind of Malian drainage SXY36 and answering in fermenting and producing hyaluronic acid
With, (1) SXY36 irradiates mutagenic and breeding through ultraviolet light, microwave and gamma-rays, and no hemolytic, HA fermentation production rate is high, inheritance stability,
Under preferred fermentation condition, shake flask fermentation yield is up to 0.731g/L, and the yield of ferment tank is up to 4.78g/L；(2) it adopts
With two sections of temperature-variable fermentations and stablize pH method, can ferment and prepare HMW HA, molecular weight is normal fermentation up to 1.89MDa
2 times of method.(3) different molecular weight HA is prepared from enzyme edman degradation Edman using fermentation liquid, the controlled enzymatic hydrolysis time can produce molecular weight and exist
HA within the scope of 0.035~1.89MDa.But the yield in the fermenter of the method only has 4.78g/L, yield is relatively
It is low.
CN1786180 discloses a kind of method for improving research on producing hyaluronic acid by fermentation method yield and molecular weight, is related to producing
The fermentation technical field of hyaluronic acid is used as using streptococcus zooepidemicus (Streptococcus zooepidemicus) H23 and is set out
Bacterial strain produces hyaluronic acid through liquid fermentation, and it is single nitrogen source, speed of agitator 180- that fermentation medium, which selects yeast powder,
Under conditions of 220rpm, the yield and molecular weight of hyaluronic acid are improved largely, the industrialized production suitable for hyaluronic acid.
But the method yield is also only 6g/L, yield also needs to further increase.
Currently, the yield of existing method production sodium hyaluronate has been unable to meet growing industry generally only in 6g/L
Production requirement.And how to be basic from fermentation medium, it is the feasible plan of prospect to improve the yield of production sodium hyaluronate
Slightly.
Summary of the invention
In view of the deficiencies of the prior art, it is an object of the invention to a kind of culture medium for producing sodium hyaluronate and its preparation sides
Method reduces the cost of industrialized production so that being improved by the yield of Hyaluronic Acid Production by Fermentation sodium.
To achieve this purpose, the present invention adopts the following technical scheme:
On the one hand, a kind of culture medium producing sodium hyaluronate, the culture medium includes following components by weight:
The culture medium of production sodium hyaluronate provided by the invention, passes through the inorganic salts of L-arginine, wheat bran and microelement
It is reasonably combined, when using streptococcus zooepidemicus H23 fermenting and producing sodium hyaluronate, the yield of sodium hyaluronate is greatly improved, is produced
Amount can reach 6.7g/L-7.2g/L.Microelement provided by the inorganic salts of microelement and L-arginine jointly promote bacterium
The metabolic process of body can especially promote metabolic process relevant to sodium hyaluronate metabolic pathway, and wheat bran is simultaneously thallus
Sufficient energy source is provided, ensure that the growth of thallus, and will not influence the inorganic salts of L-arginine and microelement
Effect is played, compared to other nitrogen sources such as dried silkworm chrysalis meal, urea, peptone etc., effect is more excellent.
In the present invention, the parts by weight of the white granulated sugar be 29-38 parts, such as can be 29 parts, 30 parts, 31 parts, 32 parts,
33 parts, 34 parts, 35 parts, 36 parts, 37 parts or 38 parts etc..
In the present invention, 7-15 parts of the parts by weight of the yeast extract, such as can be 7 parts, 8 parts, 9 parts, 10 parts, 11
Part, 12 parts, 13 parts, 14 parts or 15 parts etc..
In the present invention, the parts by weight of the buffering inorganic salts are 2.5-4 parts, such as can be 2.5 parts, 3 parts, 3.5 parts
Or 4 parts etc..
Preferably, the buffering inorganic salts include bitter salt, sodium dihydrogen phosphate dihydrate, potassium sulfate, di(2-ethylhexyl)phosphate
In hydrogen potassium or disodium hydrogen phosphate any one or at least two combination, wherein it is typical but non-limiting combination include: seven
Magnesium sulfate heptahydrate and sodium dihydrogen phosphate dihydrate；Bitter salt, sodium dihydrogen phosphate dihydrate and potassium sulfate；Potassium dihydrogen phosphate
And disodium hydrogen phosphate；Potassium sulfate, potassium dihydrogen phosphate and disodium hydrogen phosphate etc..Further preferably Magnesium sulfate heptahydrate, two hydrations
The combination of sodium dihydrogen phosphate and potassium sulfate.
In the present invention, buffering inorganic salts are mainly that culture medium provides suitable acid or alkali environment, can guarantee that thallus is closing
Eubolism is carried out under suitable environment.
In the present invention, the parts by weight of the L-arginine are 14-28 parts, such as can be 14 parts, 16 parts, 17 parts, 19
Part, 20 parts, 22 parts, 25 parts, 27 parts or 28 parts etc..
In human body, arginine is that a constituent in ornithine circulation has extremely important physiological function, essence
Propylhomoserin can effectively improve immunity, promotion immune system secretes natural killer cells, alkene element in phagocyte, white blood cell
(interleukin-1) etc. endogenous substance, is conducive to inhibiting tumor cell and pre- preventing virus infection.In addition, arginine is bird ammonia
The precursors of sour (L-ornithine) and proline (L-proline), proline are the important elements for constituting collagen, are mended
It fills arginine and largely organizes the health of maintenance to protect the needs such as severe trauma, burn, there is apparent help, while there is reduction
The effect of infection and inflammation.
In the present invention, the parts by weight of the polyether antifoam agent be 10-30 parts, such as can be 10 parts, 12 parts, 15 parts,
18 parts, 20 parts, 23 parts, 25 parts, 27 parts or 30 parts etc..
In the present invention, for using the polyether antifoam agent defoaming agent traditional relative to other, defoaming effect is more prominent, energy
The normal exchange for enough guaranteeing required gas in fermentation process, is conducive to thalli growth and metabolism.
In the present invention, the parts by weight of the wheat bran be 2.5-5 parts, such as can be 2.5 parts, 3 parts, 3.5 parts, 4 parts,
4.5 parts or 5 parts etc..
Wheat bran is wheat processing milling by products, wheat yellow, sheet or powdery.There is part plumule in the end of wheat skin (also
It is the position that wheat sprouts), the 5-10% or so of wheat skin total amount is accounted about, wheat skin contains a large amount of vitamine B group.Rich in fiber
Element and vitamin, main application have it is edible, be used as medicine, feedstuff, wine brewing etc..In the present invention by addition wheat bran, so that training
The nutritional ingredient supported in base is sufficient.
In the present invention, the parts by weight of the inorganic salts of the microelement be 1.5-3 parts, such as can be 1.5 parts, 2 parts,
2.5 parts or 3 parts etc..
Preferably, the inorganic salts of the microelement include cerous nitrate, zinc sulfate, lanthanum nitrate, praseodymium nitrate or ferrous sulfate
In any one or at least two combination, wherein it is typical but non-limiting combination include cerous nitrate and zinc sulfate；Sulfuric acid
Zinc and lanthanum nitrate；Lanthanum nitrate, praseodymium nitrate and ferrous sulfate etc. combination.
In the present invention, microelement provided in the inorganic salts of microelement can play with L-arginine collaboration and make
With the progress of promotion thallus and sodium hyaluronate associated metabolic access is able to ascend the yield of sodium hyaluronate.And if in culture medium
Lack L-arginine or microelement, then yield will appear decline.
Preferably, further include 2-4 parts of lecithin in the culture medium, such as can be 2 parts, 2.5 parts, 3 parts, 3.5 parts or 4
Part etc..
Lecithin is also known as lecithin, and main component is phosphatidyl choline, unsaturated fatty acid.Add in culture of the invention
Add lecithin, can further promote the yield of sodium hyaluronate.
Preferably, the culture medium includes following components by weight:
On the other hand, the present invention provides a kind of preparation method of culture medium as described above, the preparation method includes
Following steps:
(1) by white granulated sugar, yeast extract and wheat bran is soluble in water is mixed to get mixed solution；
(2) inorganic salts, the inorganic salts of microelement, L-arginine will be buffered and optionally lecithin is soluble in water, mixing
Obtain mixed solution；
(3) mixed solution that mixed solution that step (1) obtains, step (2) obtain is mixed with polyether antifoam agent to clear
Clear state obtains the culture medium.
In preparation method provided by the present invention, polyether antifoam agent need to be added finally into culture medium, in this way can
So that the effect of defoaming is more prominent.
Preferably, step (1) the mixed temperature be 20-40 DEG C, such as can be 20 DEG C, 23 DEG C, 25 DEG C, 28 DEG C,
30 DEG C, 32 DEG C, 33 DEG C, 35 DEG C, 36 DEG C, 38 DEG C or 40 DEG C etc..
Preferably, step (1) the mixed time be 20-200min, such as can be 20min, 50min, 80min,
100min, 120min, 15min, 180min or 200min etc..
In the present invention, the temperature mixed in step (1) is unsuitable excessively high, generally in room temperature.
Preferably, step (2) the mixed temperature be 50-80 DEG C, such as can be 50 DEG C, 55 DEG C, 60 DEG C, 65 DEG C,
70 DEG C, 75 DEG C or 80 DEG C etc..
Preferably, step (2) the mixed time be 10-30min, such as can be 10min, 12min, 15min,
18min, 20min, 23min, 25min, 27min or 30min etc..
In the present invention, mixing mainly includes inorganic salt and other material in step (2), and temperature is slightly promoted, facilitated inorganic
Salt, microelement mix well.
Preferably, step (3) the mixed temperature be 30-40 DEG C, such as can be 30 DEG C, 31 DEG C, 32 DEG C, 33 DEG C,
34 DEG C, 35 DEG C, 36 DEG C, 37 DEG C, 38 DEG C, 39 DEG C or 40 DEG C etc..
Preferably, step (3) the mixed time be 5-15min, such as can be 5min, 6min, 7min, 8min,
9min, 10min, 11min, 12min, 13min, 14min or 15min etc..
Preferably, the preparation method comprises the following steps:
(1) white granulated sugar, yeast extract and wheat bran is soluble in water, mixing 20-200min obtains mixing molten at 20-40 DEG C
Liquid；
(2) inorganic salts, the inorganic salts of microelement, L-arginine will be buffered and optionally lecithin is soluble in water, in 50-
10-30min is mixed at 80 DEG C obtains mixed solution；
(3) mixed solution and polyether antifoam agent obtained mixed solution that step (1) obtains, step (2) is at 30-40 DEG C
After lower mixing 5-15min, until clear state obtains the culture medium.
Compared with the existing technology, the invention has the following advantages:
The culture medium of production sodium hyaluronate provided by the invention, passes through the inorganic salts of L-arginine, wheat bran and microelement
It is reasonably combined, when using streptococcus zooepidemicus H23 fermenting and producing sodium hyaluronate, the yield of sodium hyaluronate is greatly improved, is produced
Amount can reach 6.7g/L-7.2g/L.Microelement provided by the inorganic salts of microelement and L-arginine jointly promote bacterium
The metabolic process of body can especially promote metabolic process relevant to sodium hyaluronate metabolic pathway, and wheat bran is simultaneously thallus
Sufficient energy source is provided, ensure that the growth of thallus, and will not influence the inorganic salts of L-arginine and microelement
Effect is played, compared to other nitrogen sources such as dried silkworm chrysalis meal, urea, peptone etc., effect is more excellent.
The present invention can promote thalli growth, improve tunning and produce by introducing microelement, precursor and promotor etc.
Amount, inhibits the generation of by-product, while can improve cell growth stablizing fermenting and producing and promote thallus breeding in storage
Rate breaches the bottleneck of Sodium Hyaluronate industry technology development, creates the internal and external environment of production more quality product, more exists
The multiple needs for meeting social development to a certain extent reduce the probability of adverse reaction generation, reduce production cost.
Specific embodiment
The technical scheme of the invention is further explained by means of specific implementation.Those skilled in the art should be bright
, the described embodiments are merely helpful in understanding the present invention, should not be regarded as a specific limitation of the invention.
Embodiment 1
Culture medium provided in this embodiment includes following components by weight
Preparation method:
(1) white granulated sugar, yeast extract and wheat bran is soluble in water, 100min, which is mixed, at 30 DEG C obtains mixed solution；
(2) by 1 part of bitter salt, 1 part of sodium dihydrogen phosphate dihydrate, 1 part of potassium sulfate, zinc sulfate, L- essence
Propylhomoserin and lecithin are soluble in water, and 20min is mixed at 60 DEG C and obtains mixed solution；
(3) mixed solution and polyether antifoam agent obtained mixed solution that step (1) obtains, step (2) is at 35 DEG C
After mixing 10min, until clear state obtains culture medium.
Embodiment 2
Preparation method:
(1) white granulated sugar, yeast extract and wheat bran is soluble in water, 20min, which is mixed, at 20 DEG C obtains mixed solution；
(2) potassium dihydrogen phosphate, lanthanum nitrate, L-arginine and lecithin is soluble in water, it is obtained in 50 DEG C of mixing next time 10min
To mixed solution；
(3) mixed solution and polyether antifoam agent obtained mixed solution that step (1) obtains, step (2) is at 30 DEG C
After mixing 5min, until clear state obtains the culture medium.
Embodiment 3
Preparation method:
(1) white granulated sugar, yeast extract and wheat bran is soluble in water, 200min, which is mixed, at 40 DEG C obtains mixed solution；
(2) disodium hydrogen phosphate, ferrous sulfate, L-arginine is soluble in water, mixing 30min obtains mixing molten at 80 DEG C
Liquid；
(3) mixed solution and polyether antifoam agent obtained mixed solution that step (1) obtains, step (2) is at 40 DEG C
After mixing 15min, until clear state obtains the culture medium.
Embodiment 4
Preparation method:
(1) white granulated sugar, yeast extract and wheat bran is soluble in water, 180min, which is mixed, at 40 DEG C obtains mixed solution；
(2) 2.1 parts of sodium dihydrogen phosphate dihydrate, 1.9 parts of potassium sulfates, L-arginine and lecithin is soluble in water, 80
20min is mixed at DEG C obtains mixed solution；
(3) mixed solution and polyether antifoam agent obtained mixed solution that step (1) obtains, step (2) is at 33 DEG C
After mixing 13min, until clear state obtains the culture medium.
Embodiment 5
Preparation method:
(1) white granulated sugar, yeast extract and wheat bran is soluble in water, 50min, which is mixed, at 40 DEG C obtains mixed solution；
(2) 1.5 parts of potassium dihydrogen phosphates, 1 part of disodium hydrogen phosphate, 1 part of zinc sulfate, 0.5 part of lanthanum nitrate, L-arginine are dissolved in
In water, 22min is mixed at 70 DEG C and obtains mixed solution；
(3) mixed solution and polyether antifoam agent obtained mixed solution that step (1) obtains, step (2) is at 33 DEG C
After mixing 14min, until clear state obtains the culture medium.
Comparative example 1
The difference of this comparative example and embodiment 1 is only that, does not include L-arginine in this comparative example, remaining is and embodiment
1 identical is prepared culture medium.
Comparative example 2
The difference of this comparative example and embodiment 1 is only that, does not include zinc sulfate in this comparative example, remaining with embodiment 1
It is identical that culture medium is prepared.
Comparative example 3
The difference of this comparative example and embodiment 1 is only that, does not include polyether antifoam agent in this comparative example, remaining with implementation
Example 1 is identical to be prepared culture medium.
Comparative example 4
The difference of this comparative example and embodiment 1 is only that the preparation method of this comparative example is directly to carry out all raw materials
Mixing, is prepared culture medium.
Using the culture medium of above-mentioned preparation as fermentation medium, fermentation test is carried out.Method are as follows: by streptococcus zooepidemicus H23
Seed is seeded in fermentation medium, carries out fermentation 16 hours, fermentation condition containing 5L culture medium in 7L fermentor are as follows:
PH7.0, speed of agitator 2000rpm, Ventilation Rate 1.0vvm.Shown in the yield of obtained sodium hyaluronate table 1 specific as follows:
Table 1
| Sample | Yield g/L |
| Embodiment 1 | 7.1 |
| Embodiment 2 | 7.0 |
| Embodiment 3 | 6.7 |
| Embodiment 4 | 6.8 |
| Embodiment 5 | 6.7 |
| Comparative example 1 | 5.1 |
| Comparative example 2 | 5.2 |
| Comparative example 3 | 5.4 |
| Comparative example 4 | 5.5 |
By the result of embodiment 1- embodiment 5 it is found that when containing lecithin in culture medium, the effect of fermentation is more excellent
It is good；And by the result of comparative example 1- comparative example 3 it is found that when being defoamed in culture medium without containing L-arginine, zinc sulfate or polyethers
When agent, the yield of sodium hyaluronate declines to a great extent；It is also unfavorable through the result of comparative example 4 it is found that when all raw materials are blended simultaneously
In the yield for promoting sodium hyaluronate, significantly declining also occurs in yield.
The Applicant declares that the culture medium of the present invention is explained by the above embodiments production sodium hyaluronate of the invention and its
Preparation method, but the invention is not limited to above-mentioned method detaileds, that is, do not mean that the present invention must rely on above-mentioned method detailed
It could implement.It should be clear to those skilled in the art, any improvement in the present invention, to each raw material of product of the present invention
Equivalence replacement and addition, the selection of concrete mode of auxiliary element etc., all fall within protection scope of the present invention and the open scope
Within.
Claims (10)
1. a kind of culture medium for producing sodium hyaluronate, which is characterized in that the culture medium includes following components by weight:
2. culture medium according to claim 1, which is characterized in that the buffering inorganic salts include bitter salt, two
In hypophosphite monohydrate sodium dihydrogen, potassium sulfate, potassium dihydrogen phosphate or disodium hydrogen phosphate any one or at least two combination.
3. culture medium according to claim 1 or 2, which is characterized in that the inorganic salts of the microelement include cerous nitrate,
In zinc sulfate, lanthanum nitrate, praseodymium nitrate or ferrous sulfate any one or at least two combination.
4. culture medium according to any one of claim 1-3, which is characterized in that further include lecithin in the culture medium
2-4 parts.
5. culture medium described in any one of -4 according to claim 1, which is characterized in that the culture medium includes by weight
Following components:
6. the preparation method of culture medium according to any one of claims 1-5, which is characterized in that the preparation method packet
Include following steps:
(1) by white granulated sugar, yeast extract and wheat bran is soluble in water is mixed to get mixed solution；
(2) inorganic salts, the inorganic salts of microelement, L-arginine will be buffered and optionally lecithin is soluble in water, be mixed to get
Mixed solution；
(3) mixed solution that mixed solution that step (1) obtains, step (2) obtain is mixed with polyether antifoam agent to clarification shape
State obtains the culture medium.
7. preparation method according to claim 6, which is characterized in that step (1) the mixed temperature is 20-40 DEG C；
Preferably, step (1) the mixed time is 20-200min.
8. preparation method according to claim 6 or 7, which is characterized in that step (2) the mixed temperature is 50-80
℃；
Preferably, step (2) the mixed time is 10-30min.
9. preparation method a method according to any one of claims 6-8, which is characterized in that step (3) the mixed temperature
It is 30-40 DEG C；
Preferably, step (3) the mixed time is 5-15min.
10. the preparation method according to any one of claim 6-9, which is characterized in that the preparation method includes following
Step:
(1) white granulated sugar, yeast extract and wheat bran is soluble in water, 20-200min, which is mixed, at 20-40 DEG C obtains mixed solution；
(2) inorganic salts, the inorganic salts of microelement, L-arginine will be buffered and optionally lecithin is soluble in water, at 50-80 DEG C
Lower mixing 10-30min obtains mixed solution；
(3) mixed solution and polyether antifoam agent obtained mixed solution that step (1) obtains, step (2) mixes at 30-40 DEG C
After closing 5-15min, until clear state obtains the culture medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811189723.5A CN109295132A (en) | 2018-10-12 | 2018-10-12 | A kind of culture medium and preparation method thereof producing sodium hyaluronate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811189723.5A CN109295132A (en) | 2018-10-12 | 2018-10-12 | A kind of culture medium and preparation method thereof producing sodium hyaluronate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109295132A true CN109295132A (en) | 2019-02-01 |
Family
ID=65162423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811189723.5A Pending CN109295132A (en) | 2018-10-12 | 2018-10-12 | A kind of culture medium and preparation method thereof producing sodium hyaluronate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109295132A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110484579A (en) * | 2019-08-27 | 2019-11-22 | 上海景峰制药有限公司 | A kind of streptococcus special culture media and preparation method thereof producing sodium hyaluronate |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4946780A (en) * | 1988-10-12 | 1990-08-07 | Denkl Kagaku Kogyo Kabushiki Kaisha | Method for producing sodium hyaluronate by fermentation method |
| CN101451114A (en) * | 2008-12-31 | 2009-06-10 | 桐乡市恒基生物科技有限公司 | Method for breeding fermentation high stability hyaluronic acid sodium bacterial |
| CN101935678A (en) * | 2009-06-30 | 2011-01-05 | 上海佰加壹医药有限公司 | Method for producing hyaluronic acid fermentation liquor |
| CN102021213A (en) * | 2009-09-17 | 2011-04-20 | 上海佰加壹医药有限公司 | Method for producing hyaluronic acid fermentation liquor by fermentation |
| CN102154401A (en) * | 2010-12-22 | 2011-08-17 | 东辰控股集团有限公司 | Fermentation technology for preparing high-molecular weight sodium hyaluronate |
| CN102242165A (en) * | 2011-05-26 | 2011-11-16 | 上海应用技术学院 | Method for producing high molecular weight sodium hyaluronate through fermentation and culture medium utilized by same |
| CN103014095A (en) * | 2011-09-21 | 2013-04-03 | 遵义医学院附属医院 | Use of MetarhiziumtaiiGYYA0601 strain in hyaluronic acid production |
| CN103320484A (en) * | 2013-06-28 | 2013-09-25 | 四川柯森油田化学有限公司 | Method for improving the fermentation yield of hyaluronic acid (HA) |
| WO2014005822A1 (en) * | 2012-07-05 | 2014-01-09 | Altergon Italia S.R.L. | Production of highly purified sodium hyaluronate (hana) with controlled molecular weight |
-
2018
- 2018-10-12 CN CN201811189723.5A patent/CN109295132A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4946780A (en) * | 1988-10-12 | 1990-08-07 | Denkl Kagaku Kogyo Kabushiki Kaisha | Method for producing sodium hyaluronate by fermentation method |
| CN101451114A (en) * | 2008-12-31 | 2009-06-10 | 桐乡市恒基生物科技有限公司 | Method for breeding fermentation high stability hyaluronic acid sodium bacterial |
| CN101935678A (en) * | 2009-06-30 | 2011-01-05 | 上海佰加壹医药有限公司 | Method for producing hyaluronic acid fermentation liquor |
| CN102021213A (en) * | 2009-09-17 | 2011-04-20 | 上海佰加壹医药有限公司 | Method for producing hyaluronic acid fermentation liquor by fermentation |
| CN102154401A (en) * | 2010-12-22 | 2011-08-17 | 东辰控股集团有限公司 | Fermentation technology for preparing high-molecular weight sodium hyaluronate |
| CN102242165A (en) * | 2011-05-26 | 2011-11-16 | 上海应用技术学院 | Method for producing high molecular weight sodium hyaluronate through fermentation and culture medium utilized by same |
| CN103014095A (en) * | 2011-09-21 | 2013-04-03 | 遵义医学院附属医院 | Use of MetarhiziumtaiiGYYA0601 strain in hyaluronic acid production |
| WO2014005822A1 (en) * | 2012-07-05 | 2014-01-09 | Altergon Italia S.R.L. | Production of highly purified sodium hyaluronate (hana) with controlled molecular weight |
| CN103320484A (en) * | 2013-06-28 | 2013-09-25 | 四川柯森油田化学有限公司 | Method for improving the fermentation yield of hyaluronic acid (HA) |
Non-Patent Citations (3)
| Title |
|---|
| 刘龙: "兽疫链球菌发酵生产透明质酸过程控制与优化", 《中国博士学位论文全文数据库 基础科学辑》 * |
| 成霞等: "高产量、高分子量透明质酸发酵条件优化", 《过程工程学报》 * |
| 郭学平等: "透明质酸的生产", 《药物生物技术》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110484579A (en) * | 2019-08-27 | 2019-11-22 | 上海景峰制药有限公司 | A kind of streptococcus special culture media and preparation method thereof producing sodium hyaluronate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Restaino et al. | High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production | |
| CN103250568A (en) | Method for preparing hericium erinaceus rich in selenium | |
| CN101760492A (en) | Method for extracting algae glycoproteins | |
| CN102533889B (en) | Method for continuously fermenting lysine | |
| CN110885870A (en) | Fermentation production method of N-acetylglucosamine | |
| CN104805143B (en) | A kind of method for preparing low molecule amount γ polyglutamic acids | |
| CN102453679B (en) | Zymotic fluid for biofermentation and preparation method thereof | |
| CN107853452A (en) | A kind of production method of additive for microbe feedstuff | |
| CN109295132A (en) | A kind of culture medium and preparation method thereof producing sodium hyaluronate | |
| CN109234334A (en) | A kind of method of fermenting and producing tremella polysaccharides and fermentation medium used | |
| CN112940945A (en) | Method for fermenting hirsutella sinensis | |
| CN102533891B (en) | Production method of lysine | |
| CN109182438B (en) | Production of vitamin B by fermentation of bacillus2Culture medium and culture method | |
| CN107574202A (en) | A kind of method for improving gentamicinB fermentation yield | |
| CN110066734A (en) | A kind of quickly breeding biological tropina culture medium | |
| CN106754401A (en) | A kind of production method of hirsutella sinensis fungal | |
| CN106591401A (en) | Fermentation promoter for improving output of gentamycin C1a, and adding method thereof | |
| CN103497988B (en) | Fermentation production method of safe efficient lactobacillus product | |
| CN1023409C (en) | Nutritive liquid | |
| CN103549621A (en) | Fermentation source material prepared from rape honey and used for producing fermented honey beverage | |
| CN107699504A (en) | A kind of Bradley yeast culture medium and preparation method thereof | |
| CN106306364A (en) | Enterococcus faecalis products for feed additive and production technology of enterococcus faecalis products | |
| CN115678785B (en) | Food-grade chlorella culture medium and culture method | |
| LU502072B1 (en) | Preparation method of clostridium butyricum compound microbial agent | |
| CN102329741B (en) | Method for producing fodder rhodotorula through mixed solid fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190201 |
|
| RJ01 | Rejection of invention patent application after publication |