CN109294946A - A kind of microbial bacterial agent and preparation method thereof improving dirt collecting pit pool ammonia nitrogen index - Google Patents
A kind of microbial bacterial agent and preparation method thereof improving dirt collecting pit pool ammonia nitrogen index Download PDFInfo
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- CN109294946A CN109294946A CN201811133468.2A CN201811133468A CN109294946A CN 109294946 A CN109294946 A CN 109294946A CN 201811133468 A CN201811133468 A CN 201811133468A CN 109294946 A CN109294946 A CN 109294946A
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/00—Biological treatment of water, waste water, or sewage
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Abstract
The invention discloses a kind of microbial bacterial agents and preparation method thereof for improving dirt collecting pit pool ammonia nitrogen index.The microbial bacterial agent for improving dirt collecting pit pool ammonia nitrogen index includes: that glycosaminoglycan chelating salt and bacillus megaterium amount to 100 parts by weight, wherein the glycosaminoglycan chelating salt is 10-30 parts by weight, and the bacillus megaterium is 70-90 parts by weight.The microbial bacterial agent of the present invention for improving dirt collecting pit pool ammonia nitrogen index is made of glycosaminoglycan chelating salt and bacillus megaterium cooperation, they are compounded has the function of Synergistic degradation ammonia nitrogen together, the contaminant degradations such as ammonia nitrogen, nitrite in water body can be converted, there is good regulation effect especially for the dirt collecting pit pool, with easy to use, the features such as method is simple, and project amount is small.
Description
Technical field
The invention belongs to water-treatment technology fields, more particularly, to a kind of microbial bacteria for improving dirt collecting pit pool ammonia nitrogen index
Agent
Background technique
As the continuous development of Chinese national economy is grown, population is increasing, and urbanization process is gradually accelerated, industry,
The continuous fast development of agricultural and other various industries, our soil, water resource are increasingly developed utilization.However, at us
While earth-shaking variation occurs for life, due to unordered exploitation, without making rational planning for, to the water resource processing in China and water resource
It protects work attention degree insufficient, results in present China much unprocessed, direct emission that industry, municipal sewage occurs,
There are many dirt collecting pit pools near population and industry intensively.As these dirt collecting pit pools are increasing, area is increased, and is led
A large amount of water resource is caused to deteriorate, whole nation majority urban groundwaters are also had correlative link in city by a degree of pollution
The processing of guarantor's technology, but the developing steps in city are rapid, and the speed of pollution is even more the imagination out of us, and the dirt collecting pit pool
The great number of issues that pollution causes makes people worried.
The purifying problem that China solves municipal sewage starts from the twentieth century seventies.The swag in some urban utilization suburbs is hollow
Ground, abandoned channel, marshland etc. are slightly rebuild or embankment is built a dam, and builds up stabilization pond, carries out purified treatment to municipal sewage.Wherein give birth to
Sewage quantity living accounts for half, remaining includes the various industrial sewages such as petroleum, chemical industry, papermaking, printing and dyeing.In recent years, the dirt collecting pit pool is water
The characteristics of a great problem of processing, the dirt collecting pit pool is scattered property, and pollution sources are wide, uncertain, without determining administering method, and receives dirt
Ammonia nitrogen index is often more difficult improvement ground in swag, because ammonia nitrogen needs a large amount of of nitrobacteria (Nitrosomas, Nitromonas)
Breeding could be grown, and the growth conditions that nitrobacteria requires is stringenter, especially in carbon source.Therefore, a kind of special needle
Extremely necessary is just become to the invention of the microbial bacterial agent of ammonia nitrogen degradation.
Summary of the invention
The invention solves first technical problem be to provide it is a kind of improve dirt collecting pit pool ammonia nitrogen index microbial bacteria
Agent.The microbial bacterial agent is ammonia nitrogen degradation bacterium, can convert the contaminant degradations such as ammonia nitrogen, nitrite in water body, especially for
There is good regulation effect on the dirt collecting pit pool, has the characteristics that nontoxic, safe and efficient, without secondary pollution.
The invention solves second technical problem be to provide it is a kind of improve dirt collecting pit pool ammonia nitrogen index microbial bacteria
The preparation method of agent.
The invention solves second technical problem be to provide a kind of improvement dirt collecting pit pool ammonia nitrogen and refer to calibration method.
In order to solve the above technical problems, the present invention adopts the following technical scheme that:
It is a kind of improve dirt collecting pit pool ammonia nitrogen index microbial bacterial agent, characterized by comprising: glycosaminoglycan chelating salt and
Bacillus megaterium amounts to 100 parts by weight, wherein the glycosaminoglycan chelating salt is 10-30 parts by weight, the huge gemma bar
Bacterium is 70-90 parts by weight；Preferably: the glycosaminoglycan chelating salt is 15-20 parts by weight, and the bacillus megaterium is
80-85 parts by weight.
A kind of preparation method for the microbial bacterial agent improving dirt collecting pit pool ammonia nitrogen index, it is characterised in that including walking as follows
Rapid: the glycosaminoglycan chelating salt that will amount to 100 parts by weight is uniformly mixed with bacillus megaterium, wherein the glycosaminoglycan
Chelating salt is 10-30 parts by weight, and the bacillus megaterium is 70-90 parts by weight；Preferably: the glycosaminoglycan chelating salt
For 15-20 parts by weight, the bacillus megaterium is 80-85 parts by weight.
Further improvement as technical solution, it is preferred that the chemical structure of the glycosaminoglycan chelating salt is six carbon
The polymer of sugar, for molecular weight between 1,000,000-200 ten thousand, basic unit is acetylglucosamine, is by 1000~3000 second
Polymer made of acyl Glucosamine residues are connected with each other by β (1 → 4) glucoside chain, deacetylation >=80%.
As the further improvement of technical solution, the bacillus megaterium preferable separate receives dirt screened from the high ammonia nitrogen of target
Swag water body.
It, can be in degradation dirt collecting pit pool water body rapidly and efficiently after the bacillus megaterium and the combination of glycosaminoglycan chelating salt
The indexs such as ammonia nitrogen, total phosphorus, easy to use, method is simple, forms a kind of micro- life of excellent degradation dirt collecting pit pool ammonia nitrogen index
Object microbial inoculum.
A kind of improvement dirt collecting pit pool ammonia nitrogen refers to calibration method, the method is characterized in that, it is used in the water body of the dirt collecting pit pool
The microbial bacterial agent of improvement dirt collecting pit pool ammonia nitrogen index 3-8ppm of the invention.
Compared with prior art, the invention has the following beneficial effects:
The present invention is directed to dirt collecting pit pool water body ammonia nitrogen index superelevation problem, by glycosaminoglycan chelating salt and has ammonia nitrogen degradation
The bacillus megaterium of effect compounds mixing, can rapidly and efficiently improve the indexs such as ammonia nitrogen, the total phosphorus in the water body of the dirt collecting pit pool.This
Microbial bacterial agent is easy to use, and method is simple, and project amount is small, can be suitble to the various dirt collecting pit pools.
Specific embodiment
Description of specific embodiments of the present invention below.Unless otherwise specified, each raw material in the present invention
It is obtained by commercially available purchase, equipment used in the present invention can be used conventional equipment in fields or referring to fields
The prior art carries out.The calculation of the dosage of each ingredient used in the present invention are as follows: calculated by each composition quality divided by water body volume
The concentration (ppm) of each ingredient is obtained, water body volume utilizes existing GPS according to relief form, storage capacity etc. by field technician
The area and the depth of water of positioning system and the ultrasonic water level monitoring irregular swag of system measurement are calculated.
The microbial bacterial agent of improvement dirt collecting pit pool ammonia nitrogen index of the invention is in terms of total 100 parts by weight, including weighs as follows
The component of amount number: 10-30 parts of glycosaminoglycan chelating salt, 70-90 parts of bacillus megaterium；Preferably include following parts by weight
Component: 15-20 parts of glycosaminoglycan chelating salt, 80-85 parts of bacillus megaterium.When glycosaminoglycan chelating salt be less than 10 parts by weight,
Bacillus megaterium is more than 90 parts by weight or glycosaminoglycan chelating salt is more than 30 parts by weight, and bacillus megaterium is less than 70 weights
Measure part when, can not obtain as the present invention improve dirt collecting pit pool ammonia nitrogen be less than, the excellent effect in total phosphorus, COD index.
Glycosaminoglycan chelating salt has excellent bio-compatibility, and biodegradable, catabolite is safe and non-toxic, and has
The characteristics such as anti-oxidant, antitoxin, antibacterial.Glycosaminoglycan effect described herein is that may produce to microbial bacterial agent in the dirt collecting pit pool
Raw interference, as heavy metal, antibiotic, antibacterial agent are degraded.
The glycosaminoglycan chelating salt can choose various suitable glycosaminoglycan chelating salts.Preferably, of the invention
The chemical structure of glycosaminoglycan chelating salt is the polymer of hexose, and for molecular weight between 1,000,000-200 ten thousand, basic unit is second
Acyl gucosamine is gathered made of being connected with each other as 1000~3000 acetyl glucoseamin residues by β (1 → 4) glucoside chain
Close object, deacetylation >=80%.
Bacillus megaterium is the aerobic production spore of one kind, gram-positive bacterium, has 100 years of researches history so far.
China starts from five sixties of 20th century to the research of bacillus megaterium, and the life of phosphate-solubilizing bacteria fertilizer is used for as phosphate solubilizing bacteria
It produces.At present to bacillus megaterium research gradually from traditional bacterial screening, fermentation condition optimization and biochemical functions to
The research of specific function, such as purify water, the synthesis of degradation of pesticide, microbial-bacterial fertilizer, antibiotic, enzyme industry.
Acquisition can be bought or be made by oneself to the bacillus megaterium by commercially available.It is preferred that bacillus megaterium of the present invention
Separation screening has more preferably high ammonia nitrogen dirt collecting pit pool water body by the bacterial strain that screening obtains from target high ammonia nitrogen dirt collecting pit pool water body
Improvement.
The method for screening the bacillus megaterium may include steps of:
1. taking the water sample 5mL of target high ammonia nitrogen dirt collecting pit pool water body, it is respectively connected to the 150mL equipped with 50mL enriched medium
In triangular flask, 120rpm shaking table culture culture 4 days at 30 DEG C are placed in, are trained in new enriched medium of transferring by 10% inoculum concentration
It supports, repeats aforesaid operations enrichment culture 3 times, NH cannot be utilized to eliminate4 +Microorganism, obtain with efficient ammonia nitrogen degradation bacterium be it is excellent
The enrichment culture liquid of gesture flora.
2. enrichment culture liquid is successively diluted to 10 with sterile water-5、10-6、10-7、10-8Totally 4 gradients are drawn each respectively
A dilution bacteria suspension 0.2mL, is coated on isolation medium plate, 30 DEG C of constant temperature incubations, after growing bacterium colony, chooses bacterium colony
Greatly, the bacterium colony more than quantity carries out repeating to cross isolating and purifying, until obtaining the pure culture of single strain, inclined-plane low-temperature preservation.
It is centrifuged 10min in 5000rpm 3. activating bacterium solution, supernatant is abandoned, then precipitated with 0.85% brine, mixes
It is even, it is centrifuged, is so repeated 3 times again, to remove the ammonia nitrogen generated during actication of culture.0.85% physiological saline system of thallus
Standby bacteria suspension, is inoculated into ammonia nitrogen degradation culture medium, 30 DEG C, 120rpm shaking table culture 48h by certain inoculum concentration.Pipette 10mL training
Nutrient solution is centrifuged 10min under 3000rpm, measures ammonia-nitrogen content in supernatant, and the initial ammonia nitrogen in culture medium not connect bacterium contains
Amount compares, and chooses the best bacterial strain of degradation effect.According to the cultural character and thallus colony morphology characteristic, life of this plant of separation bacterium
Biochemical reaction etc. is managed, the ammonia nitrogen screened is dropped referring to " common bacteria system identification handbook " and " Berger bacterial identification manual "
Solution bacterium carries out identification ownership, determines that the bacterial strain is bacillus megaterium, this bacterium is carried out single strain fermentation.
The microbial bacterial agent of improvement dirt collecting pit pool ammonia nitrogen index of the invention is the glycosaminoglycan that will amount to 100 parts by weight
Chelating salt is uniformly mixed with bacillus megaterium, wherein the glycosaminoglycan chelating salt is 10-30 parts by weight, the huge bud
Spore bacillus is 70-90 parts by weight；Preferably: the glycosaminoglycan chelating salt is 15-20 parts by weight, the bacillus megaterium
For 80-85 parts by weight.When glycosaminoglycan chelating salt is less than 10 parts by weight, bacillus megaterium is more than 90 parts by weight or amino
Polysaccharide chelating salt is more than that 30 parts by weight can not be obtained and be received such as the present invention in improvement when bacillus megaterium is less than 70 parts by weight
Dirty swag ammonia nitrogen is less than, the excellent effect in total phosphorus, COD index.
In the microbial bacterial agent of improvement dirt collecting pit pool ammonia nitrogen index of the invention, the bacillus megaterium and amino are more
The indexs such as ammonia nitrogen, the total phosphorus of sugared chelating salt combined in the dirt collecting pit pool water body that can rapidly and efficiently degrade, easy to use, method letter
It is single, it is a kind of microbial bacterial agent of excellent degradation dirt collecting pit pool ammonia nitrogen index.
Refer to calibration method as using this microbial bacterial agent to improve dirt collecting pit pool ammonia nitrogen, can be in dirt collecting pit pool water body
The microbial bacterial agent of the middle improvement dirt collecting pit pool ammonia nitrogen index of the invention using 3-8ppm is, it is preferable to use 5-8ppm, most preferably makes
Use 5ppm.When the dosage of the microbial bacterial agent of improvement dirt collecting pit pool ammonia nitrogen index of the invention is less than 3ppm, can not obtain
Dirt collecting pit pool ammonia nitrogen is less than, the effect of total phosphorus, COD index will cause the wave in cost when dosage is more than 8ppm for effect improvement
Take.
Embodiment
In order to illustrate more clearly of the present invention, below with reference to preferred embodiment, the present invention is described further.Ability
Field technique personnel should be appreciated that following specifically described content is illustrative and be not restrictive, this should not be limited with this
The protection scope of invention.
Embodiment 1
20 parts by weight of glycosaminoglycan chelating salt and 80 parts by weight of bacillus megaterium are uniformly mixed, improvement dirt collecting pit is made
The microbial bacterial agent 1 of pool ammonia nitrogen index.
Above-mentioned bacillus megaterium the preparation method is as follows:
1. taking the water sample 5mL of target high ammonia nitrogen dirt collecting pit pool water body, it is respectively connected to the 150mL equipped with 50mL enriched medium
In triangular flask, 120rpm shaking table culture culture 4 days at 30 DEG C are placed in, are trained in new enriched medium of transferring by 10% inoculum concentration
It supports, repeats aforesaid operations enrichment culture 3 times, NH cannot be utilized to eliminate4 +Microorganism, obtain with efficient ammonia nitrogen degradation bacterium be it is excellent
The enrichment culture liquid of gesture flora.
2. enrichment culture liquid is successively diluted to 10 with sterile water-5、10-6、10-7、10-8Totally 4 gradients are drawn each respectively
A dilution bacteria suspension 0.2mL, is coated on isolation medium plate, 30 DEG C of constant temperature incubations, after growing bacterium colony, chooses bacterium colony
Greatly, the bacterium colony more than quantity carries out repeating to cross isolating and purifying, until obtaining the pure culture of single strain, inclined-plane low-temperature preservation.
It is centrifuged 10min in 5000rpm 3. activating bacterium solution, supernatant is abandoned, then precipitated with 0.85% brine, mixes
It is even, it is centrifuged, is so repeated 3 times again, to remove the ammonia nitrogen generated during actication of culture.0.85% physiological saline system of thallus
Standby bacteria suspension, is inoculated into ammonia nitrogen degradation culture medium, 30 DEG C, 120rpm shaking table culture 48h by certain inoculum concentration.Pipette 10mL training
Nutrient solution is centrifuged 10min under 3000rpm, measures ammonia-nitrogen content in supernatant, and the initial ammonia nitrogen in culture medium not connect bacterium contains
Amount compares, and chooses the best bacterial strain of degradation effect.According to the cultural character and thallus colony morphology characteristic, life of this plant of separation bacterium
Biochemical reaction etc. is managed, the ammonia nitrogen screened is dropped referring to " common bacteria system identification handbook " and " Berger bacterial identification manual "
Solution bacterium carries out identification ownership, determines that the bacterial strain is bacillus megaterium, this bacterium is carried out single strain fermentation.
Embodiment 2
Embodiment 1 is repeated, the difference is that glycosaminoglycan chelating salt is 10 parts by weight, bacillus megaterium is 90 weight
Part, the microbial bacterial agent 2 for improving dirt collecting pit pool ammonia nitrogen index is made.
Embodiment 3
Embodiment 1 is repeated, the difference is that glycosaminoglycan chelating salt is 30 parts by weight, bacillus megaterium is 70 weight
Part, the microbial bacterial agent 3 for improving dirt collecting pit pool ammonia nitrogen index is made.
Embodiment 4
Embodiment 1 is repeated, the difference is that glycosaminoglycan chelating salt is 15 parts by weight, bacillus megaterium is 85 weight
Part, the microbial bacterial agent 4 for improving dirt collecting pit pool ammonia nitrogen index is made.
Comparative example 1
Embodiment 1 is repeated, the difference is that 100 parts by weight of glycosaminoglycan chelating salt are only included, without huge gemma bar
The microbial bacterial agent 5 for improving dirt collecting pit pool ammonia nitrogen index is made in bacterium.
Comparative example 2
Embodiment 1 is repeated, the difference is that only including 100 parts by weight of bacillus megaterium, is chelated without glycosaminoglycan
The microbial bacterial agent 6 for improving dirt collecting pit pool ammonia nitrogen index is made in salt.
Test example 1: the Baoding dirt collecting pit pool
1) three groups: blank group, control group, of the present invention group are set.
Blank group does not launch any inorganic agent；
Control group 1 launches comparative example 1, and control group 2 launches comparative example 2；
Of the present invention group of dispensing embodiment 1.
It is 5ppm that launching, which improves the amount of the microbial bacterial agent of dirt collecting pit pool ammonia nitrogen index,.
2) result: it is shown in Table 1, of the present invention group of NH3- N, total phosphorus, COD are significantly lower than blank group and control group.
1 each group NH of table3The indexs such as-N, total phosphorus, COD
| Blank group | Control group 1 | Control group 2 | Of the present invention group | |
| NH3-N(mg/L) | 3.34 | 3.12 | 2.83 | 1.82 |
| Total phosphorus (in terms of P, mg/L) | 0.82 | 0.79 | 0.61 | 0.34 |
| COD(mg/L) | 162.54 | 153.39 | 123.77 | 39.91 |
Note: NH3- N measurement uses Berthelot spectrophotometry；Total phosphorus determination uses ammonium molybdate spectrophotometric method；COD is surveyed
Surely it is measured using COD Quick testing instrument.
Test example 2: another dirt collecting pit pool in Baoding
1) three groups: blank group, control group, of the present invention group are set.
Blank group does not launch any inorganic agent；
Control group 1 launches comparative example 1, and control group 2 launches comparative example 2；
Of the present invention group of dispensing embodiment 2.
It is 5ppm that launching, which improves the amount of the microbial bacterial agent of dirt collecting pit pool ammonia nitrogen index,.
2) result: 2 are shown in Table, of the present invention group of NH3- N, total phosphorus, COD are significantly lower than blank group and control group.
2 each group NH of table3The indexs such as-N, total phosphorus, COD
| Blank group | Control group 1 | Control group 2 | Of the present invention group | |
| NH3-N(mg/L) | 3.68 | 3.52 | 2.95 | 1.72 |
| Total phosphorus (in terms of P, mg/L) | 0.65 | 0.61 | 0.51 | 0.35 |
| COD(mg/L) | 155.06 | 140.43 | 110.41 | 39.56 |
Note: NH3- N measurement uses Berthelot spectrophotometry；Total phosphorus determination uses ammonium molybdate spectrophotometric method；COD is surveyed
Surely it is measured using COD Quick testing instrument.
Test example 3: the Tianjin dirt collecting pit pool
1) three groups: blank group, control group, of the present invention group are set.
Blank group does not launch any inorganic agent；
Control group 1 launches comparative example 1, and control group 2 launches comparative example 2；
Of the present invention group of dispensing embodiment 3.
It is 5ppm that launching, which improves the amount of the microbial bacterial agent of dirt collecting pit pool ammonia nitrogen index,.
2) result: 3 are shown in Table, of the present invention group of NH3- N, total phosphorus, COD are significantly lower than blank group and control group.
3 each group NH of table3The indexs such as-N, total phosphorus, COD
Note: NH3- N measurement uses Berthelot spectrophotometry；Total phosphorus determination uses ammonium molybdate spectrophotometric method；COD is surveyed
Surely it is measured using COD Quick testing instrument.
Test example 4: the Shandong Province Rizhao City dirt collecting pit pool
1) three groups: blank group, control group, of the present invention group are set.
Blank group does not launch any inorganic agent；
Control group 1 launches comparative example 1, and control group 2 launches comparative example 2；
Of the present invention group of dispensing embodiment 4.
It is 5ppm that launching, which improves the amount of the microbial bacterial agent of dirt collecting pit pool ammonia nitrogen index,.
2) result: 4 are shown in Table, of the present invention group of NH3- N, total phosphorus, COD are significantly lower than blank group and control group.
4 each group NH of table3The indexs such as-N, total phosphorus, COD
| Blank group | Control group 1 | Control group 2 | Of the present invention group | |
| NH3-N(mg/L) | 2.94 | 2.86 | 2.68 | 1.64 |
| Total phosphorus (in terms of P, mg/L) | 0.75 | 0.63 | 0.51 | 0.34 |
| COD(mg/L) | 178.19 | 152.37 | 129.25 | 31.08 |
Note: NH3- N measurement uses Berthelot spectrophotometry；Total phosphorus determination uses ammonium molybdate spectrophotometric method；COD is surveyed
Surely it is measured using COD Quick testing instrument.
It in summary it can be seen, the microbial bacterial agent that dispensing of the invention improves dirt collecting pit pool ammonia nitrogen index passes through glycosaminoglycan
The compounding of chelating salt and bacillus megaterium can effectively improve dirt collecting pit pool ammonia nitrogen, total phosphorus, COD index, and the two, which has, coordinates to match
Cooperation is used, and in using the identical situation of total amount, the technical effect of technical solution of the present invention is better than exclusive use aminopolysaccharide salts
Or the effect of bacillus megaterium.
The above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be to the present invention
Embodiment restriction.For those of ordinary skill in the art, it can also make on the basis of the above description
Other various forms of variations or variation.Here all embodiments can not be exhaustive.It is all to belong to technology of the invention
The obvious changes or variations that scheme is extended out are still in the scope of protection of the present invention.
Claims (9)
1. a kind of microbial bacterial agent for improving dirt collecting pit pool ammonia nitrogen index, characterized by comprising: glycosaminoglycan chelating salt and huge
Bacterium anthracoides amount to 100 parts by weight, wherein
The glycosaminoglycan chelating salt is 10-30 parts by weight, and the bacillus megaterium is 70-90 parts by weight.
2. the microbial bacterial agent according to claim 1 for improving dirt collecting pit pool ammonia nitrogen index, which is characterized in that
The glycosaminoglycan chelating salt is 15-20 parts by weight, and the bacillus megaterium is 80-85 parts by weight.
3. the microbial bacterial agent according to claim 1 or 2 for improving dirt collecting pit pool ammonia nitrogen index, which is characterized in that described
The chemical structure of glycosaminoglycan chelating salt is the polymer of hexose, and for molecular weight between 1,000,000-200 ten thousand, basic unit is second
Acyl gucosamine is gathered made of being connected with each other as 1000~3000 acetyl glucoseamin residues by β (1 → 4) glucoside chain
Close object, deacetylation >=80%.
4. the microbial bacterial agent according to claim 1 or 2 for improving dirt collecting pit pool ammonia nitrogen index, which is characterized in that described
Bacillus megaterium separation screening is from target high ammonia nitrogen dirt collecting pit pool water body.
5. a kind of preparation method for the microbial bacterial agent for improving dirt collecting pit pool ammonia nitrogen index, it is characterised in that include the following steps:
The glycosaminoglycan chelating salt for amounting to 100 parts by weight is uniformly mixed with bacillus megaterium, wherein
The glycosaminoglycan chelating salt is 10-30 parts by weight, and the bacillus megaterium is 70-90 parts by weight.
6. the preparation method of the microbial bacterial agent according to claim 5 for improving dirt collecting pit pool ammonia nitrogen index, feature exist
In:
The glycosaminoglycan chelating salt is 15-20 parts by weight, and the bacillus megaterium is 80-85 parts by weight.
7. the microbial bacterial agent according to claim 5 or 6 for improving dirt collecting pit pool ammonia nitrogen index, which is characterized in that described
The chemical structure of glycosaminoglycan chelating salt is the polymer of hexose, and for molecular weight between 1,000,000-200 ten thousand, basic unit is second
Acyl gucosamine is gathered made of being connected with each other as 1000~3000 acetyl glucoseamin residues by β (1 → 4) glucoside chain
Close object, deacetylation >=80%.
8. the microbial bacterial agent according to claim 5 or 6 for improving dirt collecting pit pool ammonia nitrogen index, which is characterized in that described
Bacillus megaterium separation screening is from target high ammonia nitrogen dirt collecting pit pool water body.
9. a kind of improvement dirt collecting pit pool ammonia nitrogen refers to calibration method, which is characterized in that weighed in the water body of the dirt collecting pit pool using 3-8ppm
Benefit require any one of 1~4 described in improve dirt collecting pit pool ammonia nitrogen index microbial bacterial agent.
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| CN109897802A (en) * | 2019-03-15 | 2019-06-18 | 北京天诚众合科技发展有限公司 | A kind of complex microorganism, preparation method and the application of efficient degradation ammonia nitrogen |
| CN110791447A (en) * | 2019-11-21 | 2020-02-14 | 河北建设集团安装工程有限公司 | Compound microbial agent for improving water body sediment and preparation method and application thereof |
| CN110804565A (en) * | 2019-11-21 | 2020-02-18 | 河北建设集团安装工程有限公司 | Microbial agent for improving total phosphorus index of polluted water body and preparation method and application thereof |
| CN110835170A (en) * | 2019-11-21 | 2020-02-25 | 众合发(北京)生物科技发展有限公司 | Compound for degrading pesticide residues in water body and preparation method and application thereof |
| CN111073658A (en) * | 2019-11-21 | 2020-04-28 | 河北建设集团安装工程有限公司 | Heavy metal contaminated soil remediation agent and preparation method and application thereof |
| CN111925964A (en) * | 2020-08-21 | 2020-11-13 | 扬州大学 | Bacillus megaterium and application thereof |
| CN111995065A (en) * | 2020-08-24 | 2020-11-27 | 河北雄安迈嵘环保科技有限公司 | Preparation for improving ammonia nitrogen index of polluted water body and preparation method and application thereof |
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| CN111925964B (en) * | 2020-08-21 | 2022-04-22 | 扬州大学 | Bacillus megaterium and application thereof |
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