CN109294868B - Sample loading device of biological reaction container - Google Patents
Sample loading device of biological reaction container Download PDFInfo
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- CN109294868B CN109294868B CN201811142510.7A CN201811142510A CN109294868B CN 109294868 B CN109294868 B CN 109294868B CN 201811142510 A CN201811142510 A CN 201811142510A CN 109294868 B CN109294868 B CN 109294868B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
Abstract
The invention discloses a sample loading device of a biological reaction container, which comprises: the collator is used for flattening and conditioning the sample loading area of the reaction vessel and flattening and spreading the reaction vessel; a shifter for moving the uncoated region of the reaction vessel to the working area of the coater; a liquid transfer device for transferring the reaction system to the surface of the reaction vessel; and the coating device is used for coating and sealing the reaction system moved to the surface of the reaction container into the reaction chamber of the reaction container. The liquid of the reaction system is evenly coated on a carrying belt of the reaction vessel, so that the liquid drops of the reaction system are loaded into the micropores of the reaction vessel, and the micropores are sealed to form a plurality of independent reaction chambers. The sample loading device can continuously load samples, and solves the problem that the sample loading quantity of the traditional similar sample loading device is small.
Description
Technical Field
The invention relates to a biological reaction vessel loading device, in particular to a biological strip-shaped biological reaction vessel loading device.
Background
When detecting small molecules, nucleic acids, proteins and bacteria, if a biological reaction system is dispersed to a plurality of reaction holes for reaction, when the existing equipment is loaded into a reaction chamber, because of the defects of a reaction container and sample loading equipment, the existing equipment can only load a trace amount of reaction system, and because many reaction systems or samples cannot be loaded into the reaction container, the biological characteristics of the reaction systems or samples cannot be completely reflected.
Disclosure of Invention
The invention mainly solves the technical problem of providing a sample loading device for a biological reaction container, which can continuously load samples and solve the problem of small sample loading amount of the prior similar sample loading device.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a bioreactor vessel loading apparatus comprising:
the collator is used for flattening and conditioning the sample loading area of the reaction vessel and flattening and spreading the reaction vessel;
a shifter for moving the uncoated region of the reaction vessel to the working area of the coater;
a liquid transfer device for transferring the reaction system to the surface of the reaction vessel;
and the coating device is used for coating and sealing the reaction system moved to the surface of the reaction container into the reaction chamber of the reaction container.
Furthermore, the reaction container comprises a carrier tape and a base tape, wherein the carrier tape is provided with a plurality of micropores, the base tape covers the surface of the carrier tape with the micropores, and the base tape is combined with the micropores of the carrier tape to form a closed reaction chamber.
Further, the base band is combined with the carrier band through the coating device, the micropores form a closed reaction chamber, and the normal angle of the combination position of the base band and the carrier band is 0-90 degrees, preferably 60 degrees.
Further, the base band and the carrier band are fixed together in a manner of illumination, heating, bonding and clamping, so that the base band and the carrier band cannot move mutually, a combined body is formed, and the reaction chamber is sealed.
Further, the device also comprises a blade, and the blade moves the reaction system liquid preset in the sample loading area of the reaction vessel from one direction to another direction or multiple directions, so that the reaction system is uniformly distributed in the sample loading area of the reaction vessel in advance.
Further, the blade is divided into a scraper and an air knife according to whether the blade is in contact with the base band or not;
the normal included angle between the scraper and the carrier tape of the reaction container is 15-135 degrees, and the preferred value is 45 degrees;
the included angle between the air knife and the sample loading area of the reaction container is 90-180 degrees, and preferably 135 degrees; the included angle between the sample loading area of the reaction container and the horizontal plane is 0-45 degrees, and preferably 30 degrees.
Further, the contact angle of the scraper is 60-140 degrees, preferably 110 degrees; the air knife blows air pressure to the sample loading area, the pressure of the air pressure is adjustable, the shape of the sample loading area blown by the air pressure is a point shape, a linear shape or a gradient surface shape, and the linear air knife is preferred.
Further, the finisher can also adjust the temperature of the sample loading area.
Further, the collator has resistance wheels that flatten the reaction vessels in the area between the displacer and the collator, and the collator may have a liner that can be set at a constant temperature, preferably a constant temperature liner.
The invention has the beneficial effects that: the reaction vessel is continuously fed under the drive of the shifter, the pipettor can also continuously move liquid, the blade continuously pre-disperses the reaction system, the coater continuously coats and seals the reaction chamber, the structure is compact, the continuous sample loading can be realized, the operation space is small, the pollution possibility is low, the sample loading can be realized in a large amount, the air knife is optimized, other auxiliary equipment is not in contact with the reaction system except the necessary pipettor and the reaction vessel, the pollution possibility is reduced, and no additional auxiliary consumable material is provided. The liquid transfer device has high operation efficiency, local constant temperature protection sample introduction conditions and good sample introduction uniformity and consistency.
Drawings
FIG. 1 is a schematic view of a loading device according to the present invention;
the parts in the drawings are numbered as follows: the device comprises a finisher 1, a shifter 2, a liquid transfer device 3, an applicator 4, a blade 5, a base band 6, a carrier band 7, light 8, a reaction system 9 and micropores 10.
Detailed Description
Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. In the following description and in the drawings, the same numbers in different drawings identify the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present invention. Rather, they are merely examples of apparatus consistent with certain aspects of the invention, as detailed in the claims below. Various embodiments of the present description are described in an incremental manner.
In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
Referring to fig. 1, a bioreactor vessel loading device comprises:
the collator 1 is used for flattening and conditioning the sample loading area of the reaction vessel and flattening and spreading the reaction vessel;
a shifter 2 for shifting an uncoated region of the reaction vessel to a working region of the coater 4;
a liquid transfer device 3 for transferring the reaction system 9 to the surface of the reaction vessel;
an applicator 4 for applying and sealing the reaction system 9 moved to the surface of the reaction vessel into the reaction chamber;
the finisher 1 flattens and conditions a sample loading area of a reaction vessel, the reaction vessel is flattened and spread, a shifter 2 moves an uncoated micropore 10 area of the reaction vessel to a working area of a coater 4, a pipettor 3 moves a reaction system 9 to the surface of the reaction vessel, the coater 4 uniformly coats the reaction system 9 moved to the surface of the reaction vessel to the sample loading area of the reaction vessel through the movement of a scraper or an air knife, when the reaction vessel moves, the reaction system 9 is uniformly distributed to a reaction chamber by utilizing an included angle between a base band 6 and a carrier band 7, and the reaction chamber is sealed at the same time to form a closed reaction vessel.
Specifically, the reaction container comprises a base band 6 and a carrier band 7, wherein the carrier band 7 is provided with a plurality of micropores 10, the base band 6 finally covers the surface of the carrier band 7 with the micropores 10, the surface is combined with the micropores 10 of the carrier band 7 to form a closed reaction chamber through the micropores 10, the base band 6 is combined with the carrier band 7 through a coating device 4 to form the closed reaction chamber through the micropores 10, and the normal angle of the combination position of the base band 6 and the carrier band 7 is 0-90 degrees, preferably 60 degrees. The shape of the aperture of the micro-hole 10 is any preset shape, and can be a regular or symmetrical figure, generally a circle, a square, a rectangle, a triangle, a hexagon, a rhombus, a parallelogram, an irregular figure, and preferably a hexagon. The perimeter of the micropores 10 is 4um to 40mm, the depth of the pores is generally 1um to 10mm, and the density of the pores is generally 1-50000 pores/cm2The sizes of the two ends of the hole can be different, so that the wall of the hole is allowed to have a slope; preferably, the perimeter of the holes is 200um, the depth of the holes is 200um, and the density of the holes is 5000/cm.
When the base tape 6 and the carrier tape 7 pass through the coating device 4, the coating device tightly bonds or hooks the base tape 6 and the carrier tape 7, in some examples, the base tape 6 and the carrier tape 7 are fixed together by adopting light 8 and/or heating auxiliary combination of the coating device, so that the base tape 6 and the carrier tape 7 cannot move mutually, a combined body is formed, and the reaction chamber is sealed. Meanwhile, according to the combination mode of the base band 6 and the carrier band 7 of the reaction vessel, the sample loading device can select the light 8 to quickly combine the base band 6 and the photosensitive adhesive of the carrier band 7, which is beneficial to reinforcing the sealing of the reaction chamber; meanwhile, according to the combination mode of the base band 6 and the carrier band 7 of the reaction vessel, the applicator 4 with the heating function of the sample loading device can be selected to accelerate the bonding speed, which is beneficial to accelerating the sealing of the reaction chamber.
The applicator 4 further comprises a blade 5, and the blade 5 is used for moving the liquid of the reaction system 9 preset on the sample area of the reaction vessel from one direction to another direction or directions, so that the reaction system 9 is uniformly distributed on the sample area of the reaction vessel in advance. The blade 5 is divided into a scraper (contact) and an air knife (non-contact) according to whether the blade is in contact with the base tape 6 or not;
the length of the cutting edge of the scraper is not less than the length of the cross section of the carrier tape 7, the section of the scraper is wedge-shaped, and a flow guide groove is preferably arranged on the sample loading surface of the scraper, so that a reaction system 9 dripped on the surface of the scraper can be uniformly distributed to a wedge-shaped tool nose; the normal included angle between the scraper and the carrier tape 7 of the reaction container is 15-135 degrees, the typical value is 45 degrees, the reaction system 9 moved by the liquid shifter 3 moves along the blade direction, and then is pre-distributed to the surface of the carrier tape 7 in the moving process of the carrier tape 7, and the reaction system 9 enters the micropores 10 of the reaction container because the micropores 10 of the reaction container are hydrophilic; the contact angle of the scraper is 60-140 degrees, and preferably 110 degrees.
The included angle between the air knife and the sample loading area of the reaction container is 90-180 degrees, and preferably 135 degrees; the included angle between the sample loading area of the container and the horizontal plane is 0-45 degrees, and preferably 30 degrees. The air knife blows air pressure to the sample loading area, the pressure of the air pressure is adjustable, the shape of the sample loading area blown by the air pressure is a point shape, a linear shape or a gradient surface shape, and the linear air knife is preferred. The section of the air knife is wedge-shaped, the air knife is hollow, the wedge-shaped section of the air knife can discharge gas outwards through air flow, the discharged gas generates power, the reaction system 9 between the air knife and the reaction container is uniformly dispersed, when the reaction container and the wedge-shaped section of the air knife mutually generate displacement, the gas blown out by the air knife serves as the power to move the reaction system 9 in the opposite direction, and in the moving process, because the micropores 10 of the reaction container are hydrophilic, the reaction system 9 enters the micropores 10 of the reaction container.
The scraper and the air knife can enable the reaction system 9 to be uniformly distributed on the surface of the reaction container and enter the micropores 10, the reaction system 9 which does not enter the micropores 10 continuously moves to other reaction holes under the action of the blade edge of the scraper or the air knife, and the pre-filling of the reaction system 9 is completed, preferably the air knife.
The reaction system 9 is moved to the surface of a reaction vessel by a liquid transfer device 3, the reaction system 9 is pre-dispersed to the reaction vessel by a blade 5, the reaction vessel moves under the driving of a shifter 2, the shifter 2 and a coater 4 are respectively arranged at two sides of the reaction vessel, a connecting line of a rotating wheel of the shifter 2 and an axle center of a rotating wheel of the coater 4 is vertical to the reaction vessel, the rotating wheel of the shifter 2 belongs to a driving wheel, the reaction vessel is driven to move obliquely upwards by power, the rotating wheel of the coater 4 can be either a driving wheel or a driven wheel, preferably a driven wheel, and the pressure or/and the friction force of the two wheels are utilized to rotate along with the shifter 2. The rotating wheels of the coating device 4 have adjustable elasticity along two axis directions, so that the driven wheels of the coating device 4 and the driving wheels of the shifter 2 always keep pressure, and when the reaction vessel moves, the base belt 6 and the carrier belt 7 of the reaction vessel are tightly combined together through the pressure. The base band 6 is positioned between the carrier band 7 and the coating device 4, when the coating device 4 is driven by the wheel to rotate, the base band 6 is combined with the carrier band 7, the reaction system 9 which is not uniformly distributed is distributed to the rest reaction holes, and after the base band 6 is combined with the carrier band 7, the reaction system 9 forms reaction chambers in the micropores 10 of the base band 6 and the carrier band 7.
The reaction vessels are flatly laid on the plane of the finisher 1, the finisher 1 is provided with resistance wheels, the resistance wheels enable the reaction vessels to be flat in the area between the displacer 2 and the finisher 1, the finisher 1 can be provided with a liner, the liner can be set to be constant in temperature, and a constant-temperature liner is preferred.
The working plane of the finisher 1 has an included angle with the horizontal plane, the included angle a is generally 0-45 degrees, and preferably 30 degrees, so that the included angle between the sample loading area of the reaction vessel and the horizontal plane is the same as that between the sample loading area of the reaction vessel and the horizontal plane.
The finisher 1 is a carrying table of the carrier tape 7 and also provides a constant temperature control function, so that the reaction liquid is kept at a given temperature condition, the reaction liquid and the carrier tape 7 are kept to work under a constant temperature working condition, the temperature range of the finisher 1 is generally 4-95 degrees, preferably 25 degrees, and 95 degrees can be selected under a characteristic reaction system 9.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. A bioreactor vessel loading device, comprising:
the collator is used for flattening and conditioning the sample loading area of the reaction vessel and flattening and spreading the reaction vessel;
a shifter for moving the uncoated region of the reaction vessel to the working area of the coater;
a liquid transfer device for transferring the reaction system to the surface of the reaction vessel;
a coater for coating and sealing the reaction system moved to the surface of the reaction vessel into the reaction chamber of the reaction vessel;
the reaction container comprises a carrier band and a base band, wherein the carrier band is provided with a plurality of micropores, the base band covers the surface of the carrier band with the micropores, and the base band is combined with the micropores of the carrier band to form a closed reaction chamber;
the reaction system liquid pre-arranged in the sample loading area of the reaction vessel is moved to another direction or multiple directions by the blade, so that the reaction system is uniformly distributed in the sample loading area of the reaction vessel in advance.
2. The bioreactor vessel sampling device as claimed in claim 1, wherein the base band is combined with the carrier band by the applicator, so that the micropores form a closed reaction chamber, and the normal angle at the combination of the base band and the carrier band is 0-90 degrees.
3. The bioreactor vessel loading assembly of claim 2, wherein the normal angle at the junction of the base strip and the carrier strip is 60 degrees.
4. The bioreactor vessel sampling device of claim 1, wherein the base band and the carrier band are fixed together by light, heat, adhesive, and hook.
5. The bioreactor vessel loading device as claimed in claim 1, wherein the blade is divided into a scraper and an air knife according to whether the blade contacts the base tape;
the normal included angle between the scraper and the carrier tape of the reaction container is 15-135 degrees;
the included angle between the air knife and the sample loading area of the reaction container is 90-180 degrees; the included angle between the sample loading area of the reaction container and the horizontal plane is 0-45 degrees.
6. The bioreactor vessel sampling device of claim 5, wherein the normal included angle between the scraper and the carrier tape of the bioreactor vessel is 45 degrees, and the included angle between the air knife and the sampling area of the bioreactor vessel is 135 degrees; the sample loading area of the reaction vessel forms an angle of 30 degrees with the horizontal plane.
7. The bioreactor vessel sample loading device as claimed in claim 5, wherein the scraper antenna is 60-140; the air knife adopts air pressure to blow to the sample loading area, the pressure of the air pressure is adjustable, and the shape of the air pressure blowing to the sample loading area is in a point shape, a linear shape or a gradient surface shape.
8. The bioreactor vessel loading assembly of claim 7, wherein the scraper feeler is 110 degrees.
9. The bioreactor vessel loading assembly of claim 1, wherein the collator further adjusts the temperature of the loading zone.
10. A bioreactor vessel sample application apparatus as claimed in claim 1, wherein the collator has resistance wheels which flatten the reaction vessels in the region between the displacer and the collator, the collator having a pad which is capable of being set at a constant temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811142510.7A CN109294868B (en) | 2018-09-28 | 2018-09-28 | Sample loading device of biological reaction container |
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| CN201811142510.7A CN109294868B (en) | 2018-09-28 | 2018-09-28 | Sample loading device of biological reaction container |
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| CN109294868B true CN109294868B (en) | 2021-11-19 |
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| JPH06196548A (en) * | 1992-12-24 | 1994-07-15 | Sumitomo Metal Ind Ltd | Electrostatic chuck |
| JP4359357B2 (en) * | 1999-03-09 | 2009-11-04 | 株式会社瑞光 | Heat sealing equipment |
| CN101597566B (en) * | 2009-07-22 | 2011-12-21 | 郑州安图绿科生物工程有限公司 | Method for detecting drug sensitivity of mycobacterium tuberculosis by using closed micropore card |
| CN202201884U (en) * | 2011-08-02 | 2012-04-25 | 王开道 | Kit for cell biotechnology |
| CN102492603B (en) * | 2011-11-22 | 2013-05-01 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
| CN202485963U (en) * | 2012-03-21 | 2012-10-10 | 杜翠霞 | Novel smear-pushing apparatus for testing |
| CN203275156U (en) * | 2013-04-10 | 2013-11-06 | 上海裕隆生物科技有限公司 | Biological chip carrier fixing device of microarrayer |
| US11513333B2 (en) * | 2016-02-18 | 2022-11-29 | Optofluidics Inc. | System and method for characterizing particulates in a fluid sample |
| CN106367307A (en) * | 2016-08-30 | 2017-02-01 | 冯晓均 | Automatic nucleic acid quantitative analyzing device and analyzing method |
| US10427162B2 (en) * | 2016-12-21 | 2019-10-01 | Quandx Inc. | Systems and methods for molecular diagnostics |
| SG10201702235TA (en) * | 2017-03-20 | 2018-10-30 | Star Array Pte Ltd | Apparatus for amplification of nucleic acids |
| CN107755187B (en) * | 2017-11-15 | 2020-04-28 | 深圳市华星光电半导体显示技术有限公司 | Alignment film coating machine and coating method |
| CN207866590U (en) * | 2018-01-25 | 2018-09-14 | 苏州大学 | A kind of box for protein coating band |
| CN109342380B (en) * | 2018-09-28 | 2021-03-19 | 杭州莱约科技有限公司 | Biological detection system |
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Effective date of registration: 20211025 Address after: Room 509, unit 1, building 9, Xixi Lanhai City, Cangqian street, Yuhang District, Hangzhou City, Zhejiang Province Applicant after: Hangzhou Laiyue Technology Co.,Ltd. Address before: No.45, indigo Road, Kunyang Town, Pingyang County, Wenzhou City, Zhejiang Province Applicant before: Yang Fan |
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