CN109294867A - Emulsion preparing device - Google Patents
Emulsion preparing device Download PDFInfo
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- CN109294867A CN109294867A CN201710608310.5A CN201710608310A CN109294867A CN 109294867 A CN109294867 A CN 109294867A CN 201710608310 A CN201710608310 A CN 201710608310A CN 109294867 A CN109294867 A CN 109294867A
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- rotor plate
- adapter
- fixed
- emulsion
- chip
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
The present invention provides a kind of emulsion preparing devices, comprising: fixing seat；Emulsion chip, emulsion chip are equipped with chip liquid inlet, are installed in fixing seat；Adapter, adapter include fluid passage, and fluid passage is to be connected to external catheter and chip liquid inlet；Rotor plate, for moving the sealed connection between adapter realization adapter and chip liquid inlet;Snap-in structure, for adapter to be removably fixed on rotor plate.There is the beneficial effect for quickly and easily replacing adapter using technical solution of the present invention.
Description
Technical field
The present invention relates to gene sequencing technology fields, more particularly, it relates to a kind of emulsion preparing device.
Background technique
It, need to be by the sample making taken at emulsion, in order to the base in sample in prior art gene sequencing process
Because being sequenced.After emulsion is made to sample using emulsion preparing device, the channel to conveying sample is needed to be rinsed,
The production of subsequent emulsion is polluted to avoid a upper sample.But the adapter being connected with emulsion chip is difficult to rush
Wash clean, therefore replacing conversion head is a kind of relatively good cleaning mode.But in the prior art, conversion head generally passes through detent
The modes such as portion are securing, make replace adapter be difficult carry out or replacement operation it is cumbersome.
Summary of the invention
The purpose of the present invention is to provide a kind of emulsion preparing devices, it is intended to solve connector in emulsion preparing device and replace
It is inconvenient.
In order to realize goal of the invention, the present invention provides a kind of emulsion preparing devices, comprising: fixing seat；Emulsion chip,
The emulsion chip is equipped with chip liquid inlet, is installed in the fixing seat；Adapter, the adapter include that liquid is logical
Road, the fluid passage is to be connected to external catheter and the chip liquid inlet；Rotor plate, it is real for moving the adapter
Sealed connection between the existing adapter and the chip liquid inlet;Snap-in structure, for removably fixing adapter
In the rotor plate.
Preferably, emulsion preparing device further comprises the compression bar with fixing seat pivot joint, and fixing seat includes for solid
Determine the body surfaces of emulsion chip, the compression bar be used to press to the rotor plate for being fixed with adapter emulsion chip with
Realize the sealed connection between adapter and the chip liquid inlet.
Preferably, the rotor plate is equipped with the relief hole for accommodating adapter；The snap-in structure includes being fixed on
Detent portion and the limited block for the lateral wall for being convexly equipped in the adapter on the rotor plate；The detent portion include spacing head and
Gag lever post, the spacing head are connected to one end of the gag lever post, and the gag lever post is fixed far from the other end of the spacing head
In on rotor plate；Between the spacing head and the rotor plate to screw in/screw out the detent portion of the limited block, to tear open
Unload/fixed transfer head to rotor plate.
Preferably, further also further include overlap joint ring, the overlap joint ring positioned at the rotor plate and the limited block it
Between.
It preferably, include the detent surface opposite with rotor plate by spacing head, from the edge on the detent surface to wherein
The heart, the distance between described detent surface to rotor plate are gradually reduced.
Preferably, the limited block is equipped with the second chamfering abutted against with the spacing head.
Preferably, the limited block is the center of circle in the cyclic annular setting of fan using adapter.
Preferably, the rotor plate is correspondingly provided with the through-hole passed through for gag lever post, the detent portion further include locking cap and
Position-arresting disk；Position-arresting disk is perpendicularly fixed at the middle part of gag lever post and gag lever post passes through the center of position-arresting disk；Gag lever post is far from limit
One end of head is additionally provided with the screw thread for being connected and fixed cap, so that position-arresting disk and locking cap, which clamp rotor plate, is fixed on rotation for detent portion
On plate.
Preferably, the snap-in structure is symmetrically arranged with the two detent portions along the resigning hole peripheral, on the adapter
It is correspondingly provided with two limited blocks.
Preferably, the rotor plate is equipped with the relief hole for accommodating the adapter, and the snap-in structure includes solid
Due to two convex buckles on the rotor plate and it is convexly equipped in two fixed block of adapter outside wall surface；Two convex buckles are located at described allow
The two sides in position hole, two fixed blocks are correspondingly arranged with two convex buckles；The convex buckle includes pillar and button block, the pillar
One end is fixed on the rotor plate, and the button block is set on the other end of the pillar far from the rotor plate；Two is described solid
Determine block and is correspondingly provided with the button hole worn for convex buckle, so that the button block abuts after passing through button hole with the fixed block, it will be described
Fixed block is fixed between the button block and the rotor plate.
The present invention provides a kind of emulsion preparing devices, by making adapter pass through snap-in structure in setting snap-in structure
It is fixed on rotor plate, in order to which rotary head can be mounted and dismounted very easily.
Detailed description of the invention
Fig. 1 is that emulsion preparing device disassembles the part-structure explosive view after oily phase connector in the present embodiment.
Fig. 2 is the partial enlarged view in Fig. 1.
Fig. 3 is the schematic perspective view of another state of Fig. 1 emulsion preparing device.
Fig. 4 is top view in Fig. 1.
Fig. 5 is the cross-sectional view in Fig. 4 at A-A.
Fig. 6 is the detonation configuration enlarged drawing of adapter in Fig. 1.
Fig. 7 is the cross-sectional view of Fig. 6.
Fig. 8 is the schematic perspective view of another state after disassembling oily phase head in Fig. 1.
Fig. 9 is the partial enlarged view in Fig. 8.
Figure 10 is the structural schematic diagram of emulsion preparing device in another embodiment of the present invention.
Figure 11 is the partial enlarged view in Figure 10.
Figure 12 is the schematic perspective view of emulsion chip in one embodiment of the invention.
Figure 13 is the top layer chip architecture schematic diagram in Figure 12.
Figure 14 is the oily phase multi-layer sheet structure schematic diagram in Figure 12.
Figure 15 is the water phase multi-layer sheet structure schematic diagram in Figure 12.
Figure 16 is sectional view at C-C in Figure 12.
Figure 17 is sectional view at D-D in Figure 12.
Figure 18 is the schematic diagram of another set-up mode of Figure 17 guide angle.
Figure 19 is the schematic diagram of Figure 18 guide angle still another configuration mode.
Figure 20 is the schematic perspective view of emulsion chip in another embodiment of the present invention.
The rough schematic view of Figure 21 emulsion chip interior access of the present invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.
The invention discloses a kind of emulsion preparing devices.
As shown in Figure 1-3, emulsion preparing device includes catheter 80, emulsion chip 10, rotor plate 20, compression bar 30, turns
Connector 40, snap-in structure 50, fixing seat 60, and oily phase connector 70.
As shown in figure 3, fixing seat 60 is located at the bottom of emulsion preparing device, including the rotation branch of fixing seat main body 63, first
Platform 62, second rotates platform 64 and shaft 61.Fixing seat main body 63 is arranged in cuboid plate, and fixing seat main body 63 includes main body
Surface 631, body surfaces 631 and 63 thickness direction of fixing seat main body are perpendicular.First rotation platform 62 and the second rotation platform
64 are arranged along the length direction interval of body surfaces 631, and are convexly equipped in the side of body surfaces 631 in its longitudinal direction, make
Obtain the substantially L-shaped setting of fixing seat 60, wherein the first rotation platform 62 is relative to the second rotation platform 64 more adjacent to body table
The edge in face 631.Shaft 61 rotates platform 62 through first along the width direction of fixing seat main body 63, can rotate platform first
Rotation in 62.
Referring again to Fig. 1-3, emulsion chip 10 is in cuboid plate comprising a chip upper surface 11, table on chip
Face 11 is equipped with chip liquid inlet 12 and two chip oil phase entrances 14, the closer cream of 12 to two chip oil phase entrance of chip liquid inlet 14
The edge of turbid chip 10.
Referring again to Fig. 1-3, rotor plate 20 includes plate portion 21 and end 22.The plate that plate portion 21 is square comprising two
Opposite rotating plate side 214 and rotating plate side 214 vertical connection side 216 and rotating plate surface 213.It hangs down on rotating plate surface 213
Straight connection rotating plate side 214, connection side 216.Plate portion 21 there is also defined spaced relief hole through rotating plate surface 213
211, two mounting holes 215 and multiple through-holes 212.The through-hole 212 is arranged in around relief hole 211.Relief hole 211 is used for
Removably accommodate fixed transfer head 40.Two mounting holes 215 accommodate respectively fixes two oily phase connectors 70.22 one end of strip end connects
It is connected to the connection side 216 in plate portion 21, the other end of the end 22 far from plate portion 21 passes through shaft 61 and the first rotation 62 pivot of platform
It connects, rotates rotor plate 20 centered on shaft 61 around one end of fixing seat 60.Where the present embodiment shaft 61 and plate portion 21
Plane and fixing seat main body 63 where plane it is parallel.
As shown in fig. 6-7, adapter 40 includes head 41 and seal pressure cap 42, and head 41 is in barrel-shaped setting, including bucket
Bottom 416 and bucket wall 417.The sealed connection channel 414 that bucket wall 417 encloses, at bucket bottom 416 far from sealed connection channel 414 1
End is provided inwardly with the cavity 415 for accommodating sealing ring 418；Bucket bottom 416, which is additionally provided with, to be connected along through bucket bottom 416 with communication seals
The fluid passage 413 in channel 414 and cavity 415, fluid passage 413, which is located at, to be tightly connected between channel 414 and cavity 415.Liquid
Body channel 413 includes feed liquor port 411 and outlet end mouth 412, and feed liquor port 411 is fluid passage 413 and sealed connection channel
The port of 414 junctions, outlet end mouth 412 are the port of fluid passage 413 and 415 junction of cavity.It is tightly connected channel 414
Interior sidewall surface be equipped with internal screw thread.
The cylindrical setting of seal pressure cap 42 a comprising lower end surface 422.Seal pressure cap 42 further includes axially being arranged along it
Tube body channel 421, cylinder of the seal pressure cap 42 adjacent to lower end surface 422 be equipped with external screw thread, and seal pressure cap 42 can be housed in sealing
In interface channel 414, is cooperated and fixed by the external screw thread of the internal screw thread and seal pressure cap 42 that are tightly connected channel 414.Pipe
Body channel 421 includes the lower port 4211 towards bucket bottom, and the lower port 4211 is horn-like.
As shown in figure 5, catheter 80 includes tube body 81, and the tube head 82 positioned at 81 one end of tube body.Tube head
82 in the horn-like setting to external diffusion, and 82 outer edge of tube head is equipped with collar flange 83.And tube head 82 and lower port
4211 are adapted.
Referring again to such as Fig. 3, compression bar 30 is two, and interval is set to the two sides of the second rotation platform 64, one end of compression bar 30
It is pivotally connected with the second rotation platform 64, rotate compression bar 30 can in a manner of body surfaces 631 to be parallel to always.Wherein, compression bar
30 towards the side of body surfaces 631 include wedge type portion 31.Compression bar 30 is for pressing to the rotor plate 20 for being fixed with adapter 40
Emulsion chip 10 is to realize the sealed connection between adapter 40 and chip liquid inlet 12.
Referring again to FIGS. 5, snap-in structure 50 includes limited block 53 and detent portion 51, limited block 53 is along the radial projection in head 41
In bucket wall 417 in the outside wall surface of exit end；Detent portion 51 includes spacing head 512 and gag lever post 511,512 He of spacing head
One end of gag lever post 511 connects, and gag lever post 511 is inserted in through-hole 212 far from the other end of spacing head 512 to be fixed on plate portion
On 21.Limited block 53 has stop surface 532, and limited block 53 and the head of bucket wall 417 are neat on the axial direction of adapter 40
It is flat；Spacing head 512 has detent surface 5121, and detent surface 5121 and rotating plate surface 213 are opposite, the limited block 53 to
It screws between the spacing head 512 and the rotor plate 20 in the detent portion 51, with disassembly/fixed transfer head 40 to rotor plate 20.
As shown in Figure 1-3, during installation, chip fixed groove emulsion chip 10 being fixed in body surfaces 631
It is interior.
First two oily phase connectors 70 are arranged in the mounting hole 215 in plate portion 21 and are fixed, then towards emulsion chip 10
Rotor plate 20 is rotated, abuts against two oily phase connectors 70 with two chip oil phase entrances 14 on emulsion chip 10 respectively, at this moment,
It is substantially parallel between rotor plate 20 and emulsion chip 10.At this point, rotating compression bar towards the rotating plate surface 213 of rotor plate 20
30, it is abutted until two wedge type portions 31 are corresponding with two rotating plate sides 214 respectively, rotation compression bar 30 is then proceeded to, so that wedge type portion 31
The corner sliding that opposite rotating plate side 214 and rotating plate surface 213 connect, until wedge type portion 31 slides into rotating plate surface 213 completely
On, compression bar 30 just presses rotor plate 20 at this time, and then the exit seal of oily phase connector 70 is pressed on emulsion chip 10
On two chip oil phase entrances 14.
As illustrated in figs. 5-7, catheter 80 is inserted into tube body channel 421, the outer wall of the tube head 82 of catheter 80 just pastes
On the inner wall of tube body channel lower port 4211, the collar flange 83 of catheter 80 is attached at lower end surface 422.It then will sealing
Pressure cap 42, which screws in, to be tightly connected in channel 414, by the collar flange 83 of catheter 80 be folded in lower end surface 422 and bucket bottom 416 it
Between, realize that the tube head 82 of catheter 80 is docked with the sealing between feed liquor port 411, so that the liquid in catheter 80 flows into
In fluid passage 413.
As shown in figure 5, adapter 40 to be inserted into the relief hole 211 in plate portion 21, adapter 40 and core when installation adapter 40
Piece upper surface 11 is neighbouring, and sealing ring 418 is aligned chip liquid inlet, uses liquid port 412 and chip liquid inlet 12 is opposite.Clockwise
(or counterclockwise) rotary spacing block 53, turn to limited block 53 between spacing head 512 and rotor plate 20, spacing head 512 and limit
Position block 53 tightly abuts, and gives the power of limited block 53 1 downward (towards chip upper surface), then adapter 40 presses to emulsion
Chip 10, so that the cavity wall of sealing ring 418 and cavity 415 and the week edge of chip liquid inlet 12 be made hermetically to be fitted in one
It rises, realizes that adapter 40 is docked with the sealing of emulsion chip 10.
After installation, water phase reagent can be flowed out from conduit 80 by outlet end mouth 412, then be flowed by chip liquid inlet 12
To emulsion chip 10.When that need to dismantle, rotary spacing block 53, by limited block 53 between spacing head 512 and rotor plate 20
It produces, extracts adapter 40 upwards, adapter 40 is made to be detached from rotor plate 20.
In alternate embodiment, adapter 40 first can also be fixed to rotor plate 20, then be arranged in two oily phase connectors 70
It is in the mounting hole 215 in plate portion 21 and fixed, rotor plate 20 then is rotated towards emulsion chip 10, makes two oily phase connectors 70 and turns
Connector 40 respectively on emulsion chip 10 two chip oil phase entrances 14 and chip liquid inlet 12 abut against, at this moment, rotor plate
It is substantially parallel between 20 and emulsion chip 10.At this point, compression bar 30 is rotated towards the rotating plate surface 213 of rotor plate 20, until
Two wedge type portions 31 are corresponding with two rotating plate sides 214 respectively to abut, and rotation compression bar 30 is then proceeded to, so that wedge type portion 31 is with respect to rotating plate
The corner sliding that side 214 and rotating plate surface 213 connect is pressed at this time until wedge type portion 31 is slid into completely on rotating plate surface 213
Bar 30 just presses rotor plate 20, and then the exit seal of oily phase connector 70 is pressed on to 10 liang of chip oil phases of emulsion chip
On entrance 14, while the cavity wall of sealing ring 418 and cavity 415 and the week edge of chip liquid inlet 12 being made hermetically to be fitted in one
It rises, realizes that adapter 40 is docked with the sealing of emulsion chip 10.
It should be understood that with reference to Fig. 4-5, when limited block 53 turns between spacing head 512 and rotor plate 20, limit
It is interval setting between block 53 and rotor plate 20, limited block 53 is not abutted against with rotor plate 20.Turn since compression bar 30 is pressed in
On movable plate 20, give rotor plate 20 downwards (towards chip upper surface) power, can make in this way spacing head 512 tight against be connected to limit
On block 53, give limited block 53 1 downward power, to make adapter 40 that there is the trend moved downward, such adapter 40
It is compressed on emulsion chip 10, adapter 40 just keeps adapter 40 fixed by emulsion chip 10 and snap-in structure 50
In on rotor plate 20, so that adapter 40 is removably fixed on rotor plate 20.
As shown in Figure 1, by the way that above-mentioned snap-in structure 50 is arranged between adapter 40 and rotor plate 20, by adapter
40 are fixed on rotor plate 20, can also be with while thus can very easily installing adapter 40 on rotor plate 20
Very easily adapter 40 is disassembled from rotor plate 20.It will made in previous Sample introduction emulsion chip 10 in this way
After emulsion, the emulsion of one sample following need to be prepared, can be with to avoid pollution of the upper previous sample to one sample following
The adapter 40 easily more renewed effectively avoids polluting one sample following.
As shown in figure 3, in the present embodiment, for adapter 40 for conveying water phase reagent, conveying oil phase liquid is oily phase
Connector 70, oily phase connector 70 are fixed on rotor plate 20 by screw.Certainly, in other embodiments, oily phase connector 70 can also
To be fixed on rotor plate 20 using above-mentioned snap-in structure 50.
As illustrated in figs. 5-7, overlap joint ring 54 is additionally provided between the plate portion 21 and limited block 53, the overlap joint ring 54 is along diameter
It is convexly equipped in the lateral wall on head 41 to direction, overlaps the setting annular in shape of ring 54, the overlap joint ring 54 is erected at relief hole 211
On rotating plate surface 213.Surface opposite with the rotating plate surface 213 on ring 54 will be overlapped and be defined as overlap joint ring lower surface 541.?
In installation process, adapter 40 is inserted into plate portion 21, and overlap joint ring lower surface 541 is connected to rotating plate surface 213, and limited block 53 is not
It is contacted with the rotating plate surface 213, clockwise (or counterclockwise) rotary spacing block 53, at least part of the limited block 53 can
It screws between the spacing head 512 in the detent portion 51 and the plate portion 21 of the rotor plate 20, stop surface 532 and detent surface
5121 tightly abut, and overlap joint ring lower surface 541 is tightly abutted with rotating plate surface 213, so that adapter 40 be made to be fixed on rotor plate
On 20, limited block 53 can be more stably caught between spacing head 512 and rotor plate 20 in this way.Adapter 40 need to dismantled
When, rotary spacing block 53, so that it may so that limited block 53 skids off between spacing head 512 and rotor plate 20, then from relief hole 211
Extract adapter 40.
As Figure 8-9, from 5121 edge of detent surface to its center, between detent surface 5121 and rotor plate 20
Distance is gradually reduced.Detent surface 5121 is obliquely arranged from its edge to center in this way, makes detent surface 5121
On the whole in the umbrella stood upside down.In this way when limited block 53 is caught between spacing head 512 and rotor plate 20, limited block 53 and limit
It is not face contact between potential head 512, but the outer edge of stop surface 532 is contacted with detent surface 5121, is avoided contact with
Area is excessive, is relatively easier for limited block 53 to be transferred to between spacing head 512 and rotor plate 20 in this way, also relatively more hold
It easily skids off, further facilitates replacement adapter 40.
As Figure 8-9, the limited block, which is equipped with, abuts against the second chamfering with the spacing head, specifically, limited block 53
Outer edge be equipped with the second chamfering 531 for extending along its outer edge, when limited block 53 be located at spacing head 512 and rotor plate 20 it
Between when, the second chamfering 531 is bonded setting with detent surface 5121.By the way that the second chamfering 531 is arranged in the edge of limited block 53,
Make when limited block 53 is rotated and is caught between spacing head 512 and rotor plate 20, the second chamfering 531 is affixed with detent surface 5121
It closes, in this way it is avoided that contact area is too small between limited block 53 and spacing head 512, and then limited block 53 is avoided to be caught in and skidding off
Detent surface 5121 is scratched in the process.
As shown in figure 4, the limited block 53 is the center of circle in the cyclic annular setting of fan with adapter 40.In this way, limited block 53 is set
It sets in above-mentioned shape, it is easier to adapter 40 be installed or removed by finger rotary spacing block 53, further facilitate installation.
As shown in figure 5, further, detent portion 51 further includes position-arresting disk 513 and locking cap 514, position-arresting disk 513 is vertically solid
Due to gag lever post 511 middle part and gag lever post 511 pass through position-arresting disk 513 center；And gag lever post 511 is far from spacing head 512
One end be additionally provided with screw thread, locking cap 514 can be tightened against on gag lever post 5111 by the screw thread.In installation detent portion 51 in plate portion
When on 21, gag lever post 511 is inserted into through-hole 212, until position-arresting disk 513 is abutted with rotating plate surface 213, at this point, gag lever post 511
Threaded a part stretches out through-hole 212, then locking cap 514 is tightened in the portion of the stretching through-hole 212 of gag lever post main body 511
Divide on screw thread, until locking cap 514 is abutted with plate portion 21.It is equivalent at through-hole 212 in this way, the mode of being connected through a screw thread makes
513 clamping plate portion 21 of locking cap 514 and position-arresting disk, detent portion 51 is fixed in plate portion 21.It is of course also possible to directly in plate portion
Screw hole is set on 21, detent portion 51 is directly fixed in screw hole.
Shown in Fig. 4-5, snap-in structure 50 is symmetrically arranged with two detent portions 51 along 211 peripheral region of relief hole, right on adapter 40
Two limited blocks 53 should be equipped with.Before adapter 40 is installed on plate portion 21, detent portion 51 and two limited blocks, 53 alternate intervals are distributed in
All edges of relief hole 211.By being symmetrical arranged two detent portions 51 and two limited blocks 53, adapter 40 can be made to be fixed on plate
Stress more evenly, makes the more stable of installation when in portion 21.Rotary spacing block 53, each limited block 53 respectively with corresponding limit
First 512 match, so that adapter 40 is fixed in plate portion 21.
As shown in figs. 10-11, in another embodiment, snap-in structure 50 includes two convex buckles 55 for being fixed on rotating plate surface 213
Its two fixed block 56 of outside wall surface is convexly equipped in along 40 radial direction of adapter；Two convex buckles 55 are located at the two sides of relief hole 211,
Two fixed blocks 56 are correspondingly arranged with two convex buckles 55；Convex buckle 55 includes pillar 552 and button block 551, and one end of pillar 552, which is fixed on, to be turned
In plate surface 213, button block 551 is set to the other end of the pillar 552 far from rotating plate surface 213, and fixed block 56 offers corresponding convex buckle
55 button hole 561；When adapter 40 is installed on rotor plate 20, convex buckle 55 is inserted into button hole 561, and fixed block 56 is located at button block
Between 551 and rotor plate 20, button block 551 abuts setting with fixed block 56；Wherein, fixed block 56 includes fixed block upper surface 562,
Fixed block upper surface 562 is for fixed block 56 backwards to the surface of 20 side of rotor plate on 63 thickness direction of fixing seat main body.Specifically
The button hole 561 of fixed block 56 is directed at convex buckle 55, then convex buckle 55 is inserted into button hole 561 by ground during installation, until button
Block 551 stretches out button hole 561.Since compression bar (as shown in Figure 3) gives rotor plate 20 1 pressure towards emulsion chip 10, make
Fixed block 56 cannot be skidded off from convex buckle 55.By above-mentioned setting, make adapter 40 is stable to be fixed on rotor plate 20.When
So, fixed block 55 can be abutted with rotor plate 20, make adapter 40 is more stable to be fixed on rotor plate 20.In disassembly,
Push button block 551 to button hole 561, so that button block 551 is detached from fixed block upper surface 562, can thus extract fixed block 56 upwards,
Joint 40 is disassembled from rotor plate 20.
Wherein the specific structure of emulsion chip 10 is referring to as follows described.
In the present embodiment, as shown in figure 12, emulsion chip 10 includes top plate 15, oily phase laminate 16 and water phase laminate
17.Top plate 15, oily phase laminate 16 and water phase laminate 17 are cuboid plate of the same size.
As shown in figure 13, top plate 15 includes first through hole 151, the second through-hole 152 and the third that through-thickness opens up
Through-hole 153.Three through-hole interval settings and the side for being respectively positioned on length direction, first through hole 151 are logical relative to second, third
Hole 152,153 is more adjacent to 15 edge of top plate.Second through-hole 152 and third through-hole 153 are symmetricly set on first along its length
The two sides of through-hole 151, first through hole 151 and second, third through-hole 152,153 are equidistant.
As shown in figure 14, oily phase laminate 16 includes the upper surface 161 and lower surface 167 being oppositely arranged in a thickness direction.
Oily phase laminate 16 penetrates through upper and lower surface 161,167 and offers fourth hole 166, the first discharge orifice 164 and the second discharge orifice 165.It is described
The side of oily 16 length direction of phase laminate is arranged in fourth hole 166, and first discharge orifice 164 and the second discharge orifice 165 interval are set
It sets at the middle part of oily phase laminate 16.The upper surface 161 of oily phase laminate 16 offers the first oily phase groove 162 and the of parallel interval
Two oily phase grooves 163, the described first oily phase groove 162 are strip groove along its length with the second oily phase groove 163.It is described
The both ends of first oily phase groove 162 are respectively used to connect second through-hole 152 and the first discharge orifice 164, specifically, described first
Corresponding second through-hole 152 in one end of oily phase groove 162 is positioned adjacent to the setting of fourth hole 166, the described first oily phase groove 162
The other end connect and penetrate through the first discharge orifice 164.The both ends of described second oily phase groove 163 are respectively used to connection third through-hole
153 and second discharge orifice 165, specifically, what one end of the described second oily phase groove 163 corresponded to third through-hole 153 is positioned adjacent to the
The other end of the setting of four through-holes 166, the described second oily phase groove 163 connects and penetrates through the second discharge orifice 165.166 He of fourth hole
The distance between first oily phase groove 162 and the second oily phase groove 163 are equal.
As shown in figure 15, water phase laminate 17 includes the top surface 171 and bottom surface 175 being oppositely arranged in a thickness direction.It is described
Top surface 171 offers water phase groove 172, Oil Guide groove 173 and collecting tank 174.Water phase groove 172 is in a strip shape and along water phase
The length direction of laminate 17 extends, and water phase groove 172 includes opposite first end 1721 and second end 1724, and first end 1721 is right
Answer fourth hole 166 that the side of the length direction of water phase laminate 17 is set, second end 1724 extends in water phase laminate 17
Portion.Oil Guide groove 173 is in a strip shape, intersects vertically with the second end 1724 of water phase groove 172 and penetrates through and be symmetricly set on water phase
The two sides of groove 172.Collecting tank 174 includes along the guide groove 1741 of strip of 172 length direction of water phase groove distribution and circular
Collecting pit 1742, the second end 1724 of 1741 one end of guide groove connection perforation water phase groove 172, the other end of guide groove 1741 and collection
The connection perforation of pond 1742.
As shown in figs. 16-17, it is stacked gradually after top plate 15, oily phase laminate 16 and water phase laminate 17 being aligned fixed
Complete assembling.After assembling, top plate 15 covers upper surface 161, and first through hole 151, water phase groove 172, is led at fourth hole 166
Slot 1741 and collecting pit 1742 successively penetrate through.Second the 152, first oily phase groove 162 of through-hole, the first discharge orifice 164 and Oil Guide are recessed
One end of slot 173 successively penetrates through.The oily phase groove 163 of third through-hole 153, second, the second discharge orifice 165 and Oil Guide groove 173
The other end successively penetrates through.It forms emulsion as shown in figure 21 and prepares complete path.
When preparing emulsion using emulsion chip 10, after water phase reagent flows through fourth hole 166 from first through hole 151
Water phase groove 172 is flowed into, then water phase reagent is flowed towards water phase groove 172 and 173 intersection of Oil Guide groove.Oil phase liquid
It is divided into two-way, is separately flowed into respectively from the second through-hole 152 and third through-hole 153 mutually recessed to the first oily phase groove 162 and the second oil
Then slot 163 flows into the both ends of Oil Guide groove 173, two-way oil phase liquid by the first discharge orifice 164 and the second discharge orifice 165 respectively
Body flows in opposite directions towards the intersection of Oil Guide groove 173 and water phase groove 172.Finally, water phase reagent and two-way oil phase liquid exist
Emulsion is converged and formed in 172 intersection of Oil Guide groove 173 and water phase groove (i.e. at second end 1724), and emulsion enters guide groove
It 1741 and flows into collecting pit 1742.
As shown in figure 17, the water phase groove 172 is equipped with guide face 18, the guide face 18 at the first end 1721
It is connect with the cell wall 1722 of water phase groove 172, and the guide face 18 is obliquely installed relative to the top surface 171, while described
Guide face 18 is tilted towards the second end 1724, when guide face 18 described in the present embodiment places emulsion chip level,
Height fall is formed between the first end 1721 and second end 1724 of the water phase groove 172, to water conservancy diversion from the four-way
The water phase reagent of the outflow in hole 166.In this way, the magnetic bead in water phase reagent will edge when water phase reagent flows through first end 1721
Guide face 18 towards Oil Guide groove 172 second end 1724 flow, avoid magnetic bead and accumulated at first end 1721.
In another embodiment, as shown in figure 18, guide face 18 extends to from the first end 1721 of the water phase groove 172
Two ends 1724 are formed between the first end 1721 and second end 1724 of water phase groove 172 when so that emulsion chip level being placed
More gentle height fall can make the magnetic bead in water phase reagent in entire 172 flow process of water phase groove, can edge
Guide face 18 flow, effectively avoid magnetic bead from accumulating in water phase groove 172.
Preferably, as shown in figure 19, guide face 18 is arranged in the arcwall face of indent.By being in inner concavity by guide face 18
Arcwall face can make water phase reagent fluidly more steady at first end 1721.
Preferably, as shown in figure 19, the edge of guide face 18 and the cell wall 1722 of water phase groove 172 are tangent.Thus make
It is smoothly connected between guide face 18 and the wall surface of water phase groove 172, it is further to guarantee water phase reagent in first end 1721
Locate smooth flow.
Further, as shown in figure 19, have in one end that the fourth hole 166 is connected with the water phase groove 172
There are flare openings, the fourth hole 166 is equipped with the first chamfering 1662 towards the inner wall to stream interface.Being more advantageous in this way makes
Water phase reagent more smoothly flows into first end 1721 from first through hole 166.
Preferably, as shown in figure 19, the arc-shaped setting of the first chamfering 1662.
As shown in figure 20, in another embodiment, the emulsion chip 10 of use state, which is erect, places, the first through hole
151 and the fourth hole 166 connect after form first passage 19, through-thickness is suspended at described in the first passage 19
The partial sidewall of the first passage 19 of the notch of water phase groove 172 is defined as first passage side wall 191, the first passage side wall
191 are obliquely installed relative to the lower surface 167, so that the first through hole 151 and the second through-hole 166 are towards water phase groove
Aperture is gradually increased on direction.The passage portion side wall abutted against in first passage 19 with top surface 171 is defined as second channel
Side wall 172(is not suspended at the channel side wall of portion first channel 19 on the notch of water phase groove 172).It answers considerable
Be, first passage side wall 191 with should be with the fluid passage 413 of adapter 40 be smooth connection.In this way from adapter 40
After the water phase reagent of outflow enters first through hole 151, the magnetic bead in water phase reagent will be along 191 direction of first passage side wall
Water phase groove 172 flows, and the magnetic bead in water phase reagent is avoided to gather at the abutting of the two in first through hole 151 and adapter 40
Collection, can also be such that water phase reagent flows in first passage 19 more smooth.Wherein, the first through hole 151 and the 4th in Fig. 9
Dotted line at through-hole 166 refers to as prior art.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (10)
1. a kind of emulsion preparing device characterized by comprising
Fixing seat；
Emulsion chip, the emulsion chip are equipped with chip liquid inlet, are installed in the fixing seat；
Adapter, the adapter include fluid passage, and the fluid passage enters to be connected to external catheter and the chip
Liquid mouth；
Rotor plate realizes sealed connection between the adapter and the chip liquid inlet for moving the adapter;
Snap-in structure, for adapter to be removably fixed on the rotor plate.
2. emulsion preparing device as described in claim 1, which is characterized in that further comprise the pressure with fixing seat pivot joint
Bar, fixing seat include the body surfaces for fixing emulsion chip, and the compression bar is used to be fixed with described turn of adapter
Movable plate presses to emulsion chip to realize the sealed connection between adapter and the chip liquid inlet.
3. emulsion preparing device as described in claim 1, which is characterized in that the rotor plate is equipped with for accommodating adapter
Relief hole；The snap-in structure includes the detent portion being fixed on the rotor plate and the lateral wall for being convexly equipped in the adapter
Limited block；The detent portion includes spacing head and gag lever post, and the spacing head is connected to one end of the gag lever post, described
Gag lever post is fixed on rotor plate far from the other end of the spacing head；The limited block to screw in/screw out the detent
Between the spacing head in portion and the rotor plate, with disassembly/fixed transfer head to rotor plate.
4. the emulsion preparing device as described in claim 3, which is characterized in that it further also further include overlap joint ring, it is described to take
Ring is connect to be located between the rotor plate and the limited block.
5. emulsion preparing device as claimed in claim 3, which is characterized in that the spacing head includes the card opposite with rotor plate
Position surface, from the edge on the detent surface to its center, the distance between described detent surface to rotor plate is gradually reduced.
6. emulsion preparing device as claimed in claim 5, which is characterized in that the limited block is equipped with and offsets with the spacing head
The second chamfering connect.
7. emulsion preparing device as claimed in claim 3, which is characterized in that the limited block is the center of circle in fan ring using adapter
Shape setting.
8. such as the described in any item emulsion preparing devices of claim 3-7, which is characterized in that the rotor plate is correspondingly provided with for limit
The through-hole that position bar passes through, the detent portion further includes the position-arresting disk of locking cap sum；Position-arresting disk is perpendicularly fixed at the middle part of gag lever post
And gag lever post passes through the center of position-arresting disk；Gag lever post is additionally provided with the screw thread for being connected and fixed cap far from one end of spacing head, so that limit
Position disk and locking cap clamp rotor plate and detent portion are fixed on rotor plate.
9. emulsion preparing device as claimed in claim 3, which is characterized in that the snap-in structure is along the resigning hole peripheral pair
Claim to be equipped with the two detent portions, is correspondingly provided with two limited blocks on the adapter.
10. emulsion preparing device as described in claim 1, which is characterized in that the rotor plate is equipped with described for accommodating
The relief hole of adapter, the snap-in structure include two convex buckles being fixed on the rotor plate and are convexly equipped in adapter outside wall surface
Two fixed blocks；Two convex buckles are located at the two sides of the relief hole, and two fixed blocks are correspondingly arranged with two convex buckles；
The convex buckle includes pillar and button block, and one end of the pillar is fixed on the rotor plate, and the button block is set to described
On the other end of the pillar far from the rotor plate；
Two fixed blocks are correspondingly provided with the button hole worn for convex buckle so that the button block pass through button hole after with the fixed block
It abuts, the fixed block is fixed between the button block and the rotor plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710608310.5A CN109294867A (en) | 2017-07-24 | 2017-07-24 | Emulsion preparing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710608310.5A CN109294867A (en) | 2017-07-24 | 2017-07-24 | Emulsion preparing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109294867A true CN109294867A (en) | 2019-02-01 |
Family
ID=65167186
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710608310.5A Pending CN109294867A (en) | 2017-07-24 | 2017-07-24 | Emulsion preparing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109294867A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112708545A (en) * | 2020-12-01 | 2021-04-27 | 合肥国研汉因检测科技有限公司 | Liquid drop digital PCR detection device based on micro-fluidic chip |
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Application publication date: 20190201 |