CN109287640A - Microbicide compositions containing benziothiazolinone and isotianil - Google Patents

Abstract

The present invention provides a kind of microbicide compositions, the composition includes two kinds of effective component benziothiazolinone and isotianil, and the weight ratio between two components is 1:1~50.The present invention also provides the Preparation method and uses of the composition.Test result shows that above-mentioned bactericidal composition synergy provided by the invention is obvious, it is often more important that amount of application is reduced, and use cost reduces.The bactericidal composition can effectively prevent certain specific bacteriums or the fungal disease of crop.By compounding the fungicide of different role mechanism and the mode of action, there is good effect for expand fungicidal spectrum and delay germ resistance and improve control efficiency etc..

Description

Microbicide compositions containing benziothiazolinone and isotianil

Technical field

The invention belongs to agriculture plant protection fields, more particularly to a kind of with the bactericidal composition for improving performance, tool Say to body it is to be related to a kind of bactericidal composition comprising benziothiazolinone and isotianil.

Background technique

Benziothiazolinone (benzisothiazolinone) is a kind of novel, wide-spectrum bactericide, is mainly used for preventing and treating and treating yellow The various bacteria of melon downy mildew, pear scab, apple scab, citrus anthracnose, grapevine anthracnose etc., fungal disease.Its Bactericidal effect mechanism, main includes destroying germ cell nuclear structure, it is made to lose cardia and failure death and interference germ The metabolism of cell makes its physiologic derangement, eventually leads to dead two aspects.

Isotianil (Isotianil) is the fungicide and activator of rice blast, has induced activity, simultaneously With bactericidal activity, also there is certain insecticidal activity.With strong plant induced activity, preventative application or early in morbidity Phase uses, a variety of biotic factors and abiotic component can activated plant itself defensive Resistant reaction, that is, Systemic induction resistance, And influence multiple links of the pathogen history of life, thus make plant to a variety of fungies, bacterium, insect and virus generate wide spectrum from My protective effect.And isotianil can provide the dosage of extended residual drug effect and smaller application effective component, be suitble to cause Plant defense by the infringement of phytopathogenic fungi, bacterium and virus and insect, can not be used in the regular period after treatment Infringement of the plant not by above-mentioned harmful organisms is protected, the period for providing protection continues 1 to 10d generally after handling plant, excellent It selects 1 to 7d.Isotianil also have it is strong kill microbial activity, do not expect microorganism for directly prevention and treatment, including in following guiding principle Fungi: plasmodiophora brassicae, ovum spore fungi, chytrid, Zygomycetes, sac fungus, Basidiomycetes and deuteromycetes.Such as pythium, Phytophthora, Plasmopara, Peronospora, Erysiphe, Venturia, Caulococcus, Pyrenophora etc..Isotianil is particularly successful Ground is for preventing and treating cereal disease, such as anti-Erysiphe type, rice blast type, or prevention and treatment vinegrowing and fruits and vegetables Disease in growth.

Actual pesticide experience have shown that, repeat and single-minded apply a kind of reactive compound and carry out controlling disease in many feelings It will lead to the fast selective of the bacterial strain of disease under condition, for the risk for reducing resistance disease bacterial strain selectivity, usually make at present With the mixture of different activities compound come controlling disease.Group is carried out by the reactive compound that will there is different role mechanism Close, can delaying drug resistance generate, reduce amount of application, reduce control cost.

Summary of the invention

The purpose of the present invention is being directed to fungicide resistance and pedo relict problem in practical applications, filters out difference and kill The fungicide of bacterium principle is compounded, and a kind of new microbicide compositions is obtained, to improve bactericidal agent for preventing and treating effect, delaying drug resistance It generates, reduces amount of application, reduce control cost.

It is a further object to provide comprising two kinds of effective component benziothiazolinone and isotianil bactericidal composition Preparation method and the application in agriculture field prevention and treatment corps diseases.

The purpose of the present invention can be achieved by the following measures:

A kind of microbicide compositions, the composition include two kinds of active components of benziothiazolinone and isotianil.

Inventor is found by experiment that composition of the invention is increased for preventing and treating crops bacterium or fungal disease Effect is obvious, it is often more important that amount of application is reduced, and reduces use cost.Compound structure type containing benziothiazolinone and isotianil Difference, mechanism of action is different, and the two compounding can expand fungicidal spectrum, and can delay pathogen resistance to a certain extent Generation and development speed, and no interactions resistance between benziothiazolinone and isotianil.

Active component benziothiazolinone and the weight ratio of active component isotianil are 1:1~50, in a preferred embodiment, living Property component benziothiazolinone and active component isotianil weight ratio be 1:1~40.

In a kind of preferred scheme, the weight ratio between two component of benziothiazolinone and isotianil can arbitrarily match following 1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1 are adjusted than in the range of: 14、1:15、1:16、1:17、1:18、1:19、1:20、1:21、1:22、1:23、1:24、1:25、1:26、1:27、1:28、1: 29、1:30、1:31、1:32、1:33、1:34、1:35、1:36、1:37、1:38、1:39、1:40、1:41、1:42、1:43、1: 44,1:45,1:46,1:47,1:48,1:49,1:50 can also be matched at any of the above two and be selected in composed range It selects, these ratios can be understood as weight ratio, also may include molar ratio.

The dosage form allowed on pesticide can be made by active constituent and insecticides adjuvant or auxiliary material in composition of the invention.Into one Step, the composition forms agriculture by the active component of 1~85% parts by weight and the insecticides adjuvant of 99~15% parts by weight or auxiliary material The dosage form allowed on medicine.

The present invention provides the bactericidal compositions comprising benziothiazolinone and isotianil to prevent and treat corps diseases in agriculture field The purposes of aspect, the especially purposes in terms of the fungi or bacterial disease for preventing and treating certain crops.

Above-mentioned composition specifically may include insecticides adjuvant or auxiliary material, such as carrier, solvent, dispersing agent, wetting agent, gluing One or more of agent, thickener, adhesive, surfactant or fertilizer etc..It can be mixed during application common Auxiliary agent.

Suitable auxiliary agent or auxiliary material can be solid or liquid, they are usually common substance during formulation, Such as natural or regenerated minerals, solvent, dispersing agent, wetting agent, adhesive, thickener, adhesive.

The method of administration of the present composition includes the aerial part that composition of the invention is used for plant growth, especially It is leaf portion or blade face.It can choose seed soaking or be applied to controlling object surface.The frequency and amount of application of application depend on pathogen Biology and weather life condition.The growth place of plant, such as rice field can be soaked with the liquid preparation of composition, or Composition is applied in soil by person in solid form, and such as in granular form (soil application), composition can be by soil through planting Object root enters in plant (systemic action).

Composition of the invention can be prepared into various dosage forms pesticide-acceptable, including but not limited to missible oil, suspension Agent, wettable powder, water dispersible granules, pulvis, granula, aqua, aqueous emulsion, microemulsion, encapsulated form, mother liquor, female powder etc., In a kind of preferred embodiment, dosage form of the invention uses wettable powder, suspending agent, aqua, microemulsion.According to these compositions Property and application composition purpose to be achieved and ambient conditions, can choose composition to be sprayed, atomizing, dust, Broadcast sowing or sprinkle the method application poured or the like.

Composition of the invention can be prepared into various dosage forms by known method, can by effective component with help Agent can uniformly mix together with surfactant when needing, grind, be prepared into required agent such as solvent, solid carrier Type.

Above-mentioned solvent can be selected from aromatic hydrocarbon, preferably contain 8-12 carbon atom, such as xylene mixture or substituted benzene, phthalein Esters of gallic acid, such as two octanoic acid of Dibutyl phthalate or phthalandione, fat hydrocarbon, such as thiacyclohexane or paraffin, the ether of pure and mild ethylene glycol and they And ester, such as ethyl alcohol, ethylene glycol, ethylene glycol monomethyl；Ketone, such as cyclohexanone, highly polar solvent, such as N- methyl -2- pyrrolidines Ketone, dimethyl sulfoxide or dimethylformamide and vegetable oil or vegetable oil, such as soybean oil.

Above-mentioned solid carrier, such as usually natural mineral fillers, such as talcum, kaolinite for pulvis and dispersible agent Soil, montmorillonite or atlapulgite.In order to manage the physical property of composition, polymolecularity silicic acid or polymolecularity can also be added Adsorpting polymerization object carrier, such as granulated adsorptive carriers or non-adsorbed carrier, suitable granulated adsorptive carriers are porous types, it is such as floating Stone, bentonite or bentonite；Suitable non-adsorbed carrier such as calcite or sand.Further, it is possible to use a large amount of inorganic in nature or having The prefabricated material to granulate of machine property is as carrier, especially dolomite.

The chemical property of effective component in composition according to the present invention, suitable surfactant are sulfomethylated lignin Acid, naphthalene sulfonic acids, phenolsulfonic acid, alkali salt or amine salt, alkylaryl sulfonates, alkyl sulfate, alkylsulfonate, fat Alcohol sulfate, fatty acid and sulphated fatty alcohol glycol ether, there are also the condensation product of sulfonated naphthalene and naphthalene derivatives and formaldehyde, naphthalene or The condensation product of naphthalene sulfonic acids and phenol and formaldehyde, NONIN HS 240, ethoxylated isooctyl-phenol, octyl phenol, nonyl phenol, Alkylaryl polyglycol ether, tributyl benzene polyglycol ether, three stearyl phenyl polyglycol ethers, alkyl aryl polyether alcohol, Ethoxylated castor oil, polyoxyethylene alkyl ether, ethylene oxide condensation product, ethoxylation polyoxypropylene, laurel acid polyethylene glycol Ether acetal, sorbitol ester, lignin sulfite waste liquor and methylcellulose.

Two kinds of effective components show as synergistic effect in composition of the invention, and the activity of the composition is than using individualized The independent activity of the expected summation of activity and single compound of closing object is more significant.Synergistic effect shows as allowing amount of application Reduce, broader sterilization control spectrum, quick, more longlasting control efficiency, by a small number of applying only once or several times more preferably Control plant pest germ and broaden the possible administration interval time.These characteristics are that phytopathogen control was practiced It is especially needed in journey.

In terms of microbicide compositions of the invention can be applied to agriculture field prevention and treatment corps diseases, farming referred herein Object disease especially uncinula necator, powdery mildew of cucumber, cucumber downy mildew, balsam pear powdery mildew, graw mold of tomato, wheat white powder Disease, rice blast.But composition of the invention is other than it can prevent and treat aforementioned disease, moreover it is possible to the specific illness of prevention and treatment include but It is not limited to wheat diseases (such as: wheat scab, wheat powdery mildew, wheat rust), rice disease (such as: rice sheath blight disease, water Rice false smut, paddy bacterial streak etc.), maize diseases (such as: smut of maize, the leaf blight of corn, corn southern leaf blight), cucumber Disease (such as: powdery mildew of cucumber, cucumber anthracnose, gray mold of cucumber, cucumber bacterial angular leaf spot), tomato disease are (such as: tomato Powdery mildew, anthracnose of tomato, tomato virus disease, tomato bacterial angular leaf spot etc.), grape disease (such as: downy mildew of garpe, grape Gray mold), apple disease (such as: ring rot of apple, apple anthracnose), citrus disease (such as: citrus scab simultaneously, citrus anthrax Disease), tobacco diseases (such as: wildfire, Alternaria alternate), rape disease (such as: sclerotinia sclerotiorum), cotton disease (such as: cotton Flower verticillium wilt, cotton wilt) etc..

Other features that bactericidal composition of the invention is shown are main are as follows: 1, composition mixture of the invention have it is obvious Synergistic effect；2, since two single dose chemical structures of this composition are widely different, the mechanism of action is entirely different, and there is no hand over Mutual resistance can delay two single doses that generated resistance problem is used alone；3, composition of the invention is to crop safety, preventive effect It is good.Tests prove that microbicide compositions chemical property of the present invention is stablized, synergy is significant, shows significantly to controlling object Synergy and complementation.

Specific embodiment

In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention It is further elaborated.It should be appreciated that described herein, specific examples are only used to explain the present invention, is not used to limit The fixed present invention, any modifications, equivalent replacements and improvementsmade within the spirit and principles of the invention, etc. should be included in Within protection scope of the present invention.

Percentage is weight percentage in all formulas of following embodiment.The processing work of the various preparations of the present composition Skill is the prior art, can be varied according to different situations.

One, dosage form prepares embodiment

(1) processing and embodiment of wettable powder

Active constituent benziothiazolinone and active constituent isotianil and various auxiliary agents and filler etc. are sufficiently mixed in proportion, passed through Wettable powder is made after ultra-fine pulverizer disintegrating.

Embodiment 1:52% benziothiazolinone isotianil wettable powder (1:25)

Benziothiazolinone 2%, isotianil 50%, naphthalenesulfonic acid-formaldehyde condensate 8%, lignosulfonates 10%, magnesium silicate Aluminium 4%, kaolin complements to 100%.

Embodiment 2:76.5% benziothiazolinone isotianil wettable powder (1:50)

Benziothiazolinone 1.5%, isotianil 75%, lauryl sodium sulfate 8%, ammonium sulfate 4%, white carbon black complements to 100%.

(2) processing and embodiment of microemulsion

Benziothiazolinone and isotianil, solvent are mixed to form uniform liquid in proportion, add auxiliary agent and water, stirring is equal It is even, it can be prepared by microemulsion.

Embodiment 3:5% benziothiazolinone isotianil microemulsion (1:1)

Benziothiazolinone 2.5%, isotianil 2.5%, aliphatic alcohol sulfate 3%, potassium dihydrogen phosphate 2%, ethylene glycol 10%, water Complement to 100%.

Embodiment 4:20.5% benziothiazolinone isotianil microemulsion (1:40)

Benziothiazolinone 0.5%, isotianil 20%, lauryl sodium sulfate 8%, triphenyl phosphite 4%, propylene glycol 6%, Toluene 12%, water complements to 100%.

(3) processing and embodiment of suspending agent

By active component benziothiazolinone and isotianil, formula is pressed with each components such as dispersing agent, wetting agent, thickener and water Ratio is uniformly mixed, and after sand milling and/or high speed shear, obtains semi-finished product, and water supplement, which is uniformly mixed, after analysis filters up to outstanding Floating agent.

Embodiment 5:11% benziothiazolinone isotianil suspending agent (1:10)

Benziothiazolinone 1%, isotianil 10%, lignosulfonates 6%, lauryl sodium sulfate 8%, ammonium sulfate 3% are yellow Virgin rubber 2%, hexamethylene 10%, higher alcohols 0.3%, water complements to 100%.

Embodiment 6:42% benziothiazolinone isotianil suspending agent (1:20)

Benziothiazolinone 2%, isotianil 40%, copolymer-maleic anhydride sodium salt 8%, dialkyl succinylsuccinate sulfonated ester 8% are swollen Profit soil 6%, pectin 5%, chloroform 12%, organosilicon 0.6%, water complements to 100%.

Embodiment 7:31% benziothiazolinone isotianil suspending agent (1:30)

Benziothiazolinone 1%, isotianil 30%, styrene alkyl polyoxyethylene ether phosphate 10%, alkylphenol-polyethenoxy Formaldehyde condensate sulfonate 8%, sodium bicarbonate 5%, aluminium-magnesium silicate 4%, acetone 10%, higher alcohols 0.4%, water complements to 100%.

Two, drug effect verification test

(1) Assays Example

The incidence that whole strain blade is investigated according to test grade scale, calculates disease index and control efficiency.

Control efficiency is converted into probability value (y), liquor strength (μ g/ml) is converted into logarithm (x), with least square method Concentration EC50 in calculating virulence equation and inhibiting, the toxicity index and co-toxicity coefficient (CTC) of medicament are calculated according to Sun Yunpei's Method.

Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100

Percentage composition+B medicament toxicity index * the mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture The percentage composition of middle B

Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI)] * 100

CTC≤80, composition show as antagonism, 80 < CTC < 120, and composition shows as summation action, and CTC >= 120, composition shows as synergistic effect.

1, benziothiazolinone is compounded with isotianil tests leaf muld of tomato toxicity test

1. benziothiazolinone of table is compounded with isotianil to leaf muld of tomato toxicity test interpretation of result

Medicament title	EC50(ug/mL)	ATI	TTI	CTC
					Benziothiazolinone	6.54	100.00	\	\
Isotianil	9.18	71.24	\	\
					Benziothiazolinone: isotianil=10:1	6.31	103.67	97.39	106.45
Benziothiazolinone: isotianil=1:1	6.02	108.58	85.62	126.82
					Benziothiazolinone: isotianil=1:10	6.11	107.02	73.85	144.90
Benziothiazolinone: isotianil=1:20	5.74	113.88	72.61	156.84
					Benziothiazolinone: isotianil=1:25	5.41	120.90	72.35	167.11
Benziothiazolinone: isotianil=1:30	5.82	112.40	72.17	155.75
					Benziothiazolinone: isotianil=1:40	6.42	101.82	71.94	141.53
Benziothiazolinone: isotianil=1:50	7.40	88.33	71.80	123.01
					Benziothiazolinone: isotianil=1:60	7.92	82.60	71.71	115.18

As a result (table 1) shows in benziothiazolinone: in isotianil=1:1~50 interval ranges, benziothiazolinone and isotianil are multiple The control efficiency of pairing leaf muld of tomato significantly improves, and illustrates that the two compounding has significant synergy to make leaf muld of tomato prevention and treatment With.

(2) field efficacy verification test

Test method: it at the initial stage of a disease, carries out for the first time by spraying, carrying out being administered for second after 7 days immediately, each processing 4 A cell, each 20 square meter of cell.11 days investigation statistics incidences, 5 points of each cell are random before medicine and after second of medicine Sampling, 5 plants of crops of every investigation, the lesion area for investigating every blade in whole strain account for percentage and the classification of blade area, calculate Disease index and control efficiency.

It is expected that preventive effect (%)=X+Y-XY/100 (wherein, X, Y are single dose preventive effect)

Grade scale:

0 grade: disease-free spot；

1 grade: leaf spot lesion is less than 5, and length is less than 1cm；

3 grades: leaf spot lesion 6-10, part scab length is greater than 1cm；

5 grades: leaf spot lesion 11-25, part scab is linked to be piece, and lesion area accounts for the 10-25% of leaf area；

7 grades: leaf spot lesion 26 or more, scab is linked to be piece, and lesion area accounts for the 26-50% of leaf area；

9 grades: scab is linked to be piece, lesion area account for 50% of leaf area or more or full leaf it is withered.

1, benziothiazolinone compounds field efficacy experiment with isotianil

2 benziothiazolinone of table and isotianil mixture are to water spinach Bacterial Leaf Blight disease control efficiency

Measurement result (table 2) shows that benziothiazolinone obviously proposes water spinach Bacterial Leaf Blight preventive effect with compounding for isotianil Height illustrates that the two compounding has significant synergistic effect to water spinach Bacterial Leaf Blight.

Claims (7)

1. a kind of microbicide compositions, which is characterized in that the composition includes two kinds of active components of benziothiazolinone and isotianil, Described in two component of benziothiazolinone and isotianil between weight ratio be 1:1~50.

2. microbicide compositions according to claim 1, which is characterized in that the active component benziothiazolinone and active group The weight ratio for dividing isotianil is 1:1~40.

3. microbicide compositions according to claim 1 or 2, which is characterized in that the composition is by active constituent and pesticide The dosage form allowed on pesticide is made in auxiliary agent or auxiliary material.

4. microbicide compositions according to claim 3, which is characterized in that the composition by 1~85% parts by weight work Property component and 99~15% parts by weight insecticides adjuvant or auxiliary material the dosage form allowed on pesticide is made.

5. microbicide compositions according to claim 3 or 4, which is characterized in that the dosage form is wettable powder, micro- Emulsion or suspending agent.

6. microbicide compositions according to claim 3 or 4, which is characterized in that the insecticides adjuvant or auxiliary material is selected from One or more of carrier, solvent, dispersing agent, wetting agent, adhesive, thickener, adhesive, surfactant or fertilizer.

7. use of the composition pesticide described in any one of claim 1~6 in terms of agriculture field prevents and treats corps diseases On the way.