CN109287499B - Cat litter with pet urine protein early warning function and preparation method thereof - Google Patents
Cat litter with pet urine protein early warning function and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0155—Litter comprising organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0154—Litter comprising inorganic material
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Housing For Livestock And Birds (AREA)
Abstract
The invention discloses cat litter with a pet urine protein early warning function and a preparation method thereof. The cat litter is prepared from the following raw materials: modified fine-pore silica gel, diatomite, activated carbon, a urine protein indicator and an antibacterial agent. According to the invention, the biuret reaction is applied to the preparation process of the cat litter, and the dosage of the silica gel substrate material and the urine protein indicator is scientifically matched, so that the color reaction can be generated on abnormal urine of urine protein, and the color reaction does not exist on normal urine, therefore, the health condition of pets can be preliminarily reflected, and the pet with abnormal urine can be diagnosed and examined in time, so that an early warning effect is achieved. The invention also modifies silica gel, effectively increases the hydrophilicity, enhances the dip dyeing effect of the silica gel and the urine protein indicator, and ensures that the color reaction is obviously and easily distinguished. Meanwhile, the novel antibacterial agent is added, so that the cat litter has an obvious antibacterial effect and good persistence, and does not need to be replaced frequently. The invention has simple preparation process and low cost, and is suitable for popularization and use.
Description
Technical Field
The invention belongs to the technical field of cat litter preparation, and particularly relates to a silica gel cat litter with a cat urine protein early warning function and a preparation method thereof.
Background
The cat litter is a pet product with large consumption, the number of domestic pets such as cats and dogs is increasing along with the social development and the improvement of the living standard of people, the sanitary treatment of excrement and urine becomes an important problem for environmental protection, and the cat litter becomes a common necessary product for most families breeding the pets.
The cat litter commonly used in the market at present comprises bentonite, clay, paper scraps, wood powder, silica gel cat litter and the like, but the cat litter is not convenient for users to clean because the deodorization capability of the bentonite clay cat litter is not ideal, mildew and much dust are easily generated after the cat litter is wetted, the condensation force of the paper scraps cat litter is weak, the cat litter is easy to become paste after moisture absorption, and the like. The wood flour cat litter is more environment-friendly, but the number of manufacturers is less at present.
The silica gel cat litter is characterized in that the silica gel cat litter is used as pet padding. The silica gel cat litter is a latest and most ideal pet litter detergent, the main component of the detergent is silicon dioxide, the content of the silica gel cat litter is up to more than 98%, the silica gel cat litter is white granular in appearance, the safety degree of household use is high, and the silica gel cat litter is a cat litter product which is popular with consumers in the international market at present. The existing silica gel cat litter has the main functions of adsorption deodorization, single function and high manufacturing cost. It is necessary to develop a cat litter product which has low cost and simple preparation process, can efficiently adsorb and deodorize and can reflect abnormal health of pets.
Disclosure of Invention
In order to solve the problems of high manufacturing cost, poor performance and single function of the existing cat litter product, the invention provides the cat litter with the pet urine protein early warning function and the preparation method thereof, and the cat litter takes the fine-pore silica gel as a base material and carries out hydrophilic modification on the fine-pore silica gel to enhance the dip-dyeing effect of the protein indicator; diatomite activated carbon is added to enhance the adsorption and deodorization capacity of the silica gel cat litter; and a novel double-arc antibacterial agent is added to realize a high-efficiency antibacterial and bacteriostatic function. In order to realize the purpose of the invention, the adopted technical scheme is as follows:
the cat litter with the pet urine protein early warning function is prepared from the following raw materials in parts by weight: 60-100 parts of modified fine-pore silica gel, 5-10 parts of diatomite, 5-10 parts of activated carbon, 100-150 parts of urine protein indicator and 0.1-0.5 part of antibacterial agent; the urine protein indicator is a bromophenol blue reagent or a biuret reagent.
The bromophenol blue reagent is prepared by the following method: 0.67g of bromophenol blue is dissolved in 10ml of 0.1mol/L NaOH solution and distilled water is added to 1000 ml.
The biuret reagent is prepared by the following method: weighing 1.5g of copper sulfate and 6.0g of potassium sodium tartrate, dissolving the copper sulfate and the potassium sodium tartrate in 500mL of distilled water, adding 300mL of 10% sodium hydroxide solution into the solution under stirring, and diluting the solution to 1L by using water;
the modified fine-pore silica gel is prepared by the following method:
(1) soaking the silica gel particles in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, and drying in a vacuum drying oven at 35-40 ℃ for 30min for later use;
(2) and (2) soaking the silica gel particles obtained in the step (1) in ethyl acetate for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and curing at 37 ℃ for 30min to obtain the modified fine-pore silica gel particles.
The antibacterial agent is polyhexamethylene biguanide hydrochloride.
A preparation method of cat litter with a pet urine protein early warning function comprises the following steps:
(1) pretreatment: 0.2mol/L citric acid solution 1000m1 is added toL Na2HPO4Adjusting the pH value of the solution to be 2.1 by 20ml to prepare a buffer solution; soaking the fine-pore silica gel in hydrochloric acid solution for 30min, taking out, washing with distilled water for 3-5 times, soaking in the obtained buffer solution for 30min, taking out, washing with distilled water for 3-5 times, and air drying;
(2) preparing modified fine-pore silica gel: soaking the silica gel particles obtained in the step (1) in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, placing in a vacuum drying oven for drying at 35-40 ℃ for 30min, soaking the silica gel particles in an ethyl acetate solution for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and then curing at 37 ℃ for 30min to obtain modified fine-pore silica gel particles;
(3) preparing a urine protein indicator: preparing a biuret reagent, weighing 1.5g of copper sulfate and 6.0g of potassium sodium tartrate, dissolving with 500mL of distilled water, adding 300mL of 10% sodium hydroxide solution under stirring, and diluting with water to 1L; preparing a bromophenol blue reagent, namely weighing 0.67g of bromophenol blue, dissolving the bromophenol blue in 10ml of 0.1mol/L NaOH solution, and adding distilled water to 1000 ml;
(4) weighing modified pore silica gel and biuret urine protein indicator according to a ratio, soaking the modified pore silica gel in biuret urine protein indicator solution, stirring, heating and stirring at 50 ℃ for 2-5h, filtering to separate out silica gel particles, and placing the silica gel particles in a vacuum drying oven for vacuum drying to obtain a primary finished product A1; weighing modified porous silica gel and bromophenol blue urine protein indicator according to a ratio, soaking the modified porous silica gel in bromophenol blue urine protein indicator solution, stirring, heating and stirring at 50 ℃ for 2-5h, filtering to separate out silica gel particles, and placing in a vacuum drying oven for vacuum drying to obtain a primary finished product A2;
(5) mixing the diatomite, the activated carbon and the primary finished product A1 obtained in the step (4) according to the weight, and then placing the mixture into a stirrer for homogenization to obtain mixed particles B1;
(6) dissolving the antibacterial agent in 50-100 times of distilled water by weight to obtain an antibacterial agent solution; placing the mixed particles B1 obtained in the step (5) in a rotating disc, uniformly spraying an antibacterial agent solution on the rotating disc, placing the particles in a vacuum drying oven for drying after spraying is finished until the water content is 4-8%, and then packaging to obtain the finished cat litter C1;
(7) mixing the diatomite, the activated carbon and the primary finished product A2 obtained in the step (4) according to the weight, and then placing the mixture into a stirrer for homogenization to obtain mixed particles B2;
(8) dissolving the antibacterial agent in 50-100 times of distilled water by weight to obtain an antibacterial agent solution; and (3) placing the mixed particles B2 obtained in the step (A) in a rotating disc, uniformly spraying an antibacterial agent solution on the mixed particles B2, placing the particles in a vacuum drying oven to be dried after the spraying is finished until the water content is 4-8%, and then packaging to obtain the finished cat litter C2.
The mass fraction of the ethyl acetate solution in the step (2) is 60-80%.
In the step (3), the temperature of the vacuum drying oven is 100 ℃, and the drying time is 12 hours.
When the cat litter with the pet urine protein early warning function is used, a color comparison card can be manufactured for convenience of use so as to quickly compare color changes. The color chart can be prepared by the following method:
(1) photographing the finished cat litter C1 and importing the photographed cat litter into a computer; taking urine protein overproof cat urine and normal cat urine to respectively soak finished cat litter C1, taking a picture of a color change result, guiding the picture into a computer, carrying out color difference inspection on the picture in the computer, and analyzing to obtain corresponding RGB values; manufacturing a color comparison card according to the corresponding RGB value;
(2) photographing the finished cat litter C2 and importing the photographed cat litter into a computer; taking urine protein overproof cat urine and normal cat urine to respectively soak finished cat litter C2, taking a picture of a color change result, guiding the picture into a computer, carrying out color difference inspection on the picture in the computer, and analyzing to obtain corresponding RGB values; and manufacturing a color comparison card according to the corresponding RGB value.
The method for detecting the chromatic aberration comprises the following steps:
(1) generating an electronic Lauer color comparison card in a computer according to the RGB values of all colors in the Lauer color comparison card;
(2) placing the cat litter to be photographed and the real object Lauer colorimetric card together, photographing in the same environment, and then importing the photographed cat litter and the real object Lauer colorimetric card into a computer; and then verifying whether the RGB value of the real object Lauer colorimetric card is consistent with the RGB value of the standard Lauer colorimetric card of the electronic edition, and if so, showing that the RGB value of the photo of the shot cat litter is not different.
During practical application, the color change of the silica gel after the pet excretes and uses is compared with a colorimetric card, whether the urine protein content in the urine of the pet is normal or not is judged quickly, the biuret reagent is used as a urea protein indicator, and when the urine protein exceeds the standard, the silica gel can be changed into purple from blue. When the urine protein exceeds the standard, the silica gel changes from yellow to blue by taking bromophenol blue reagent as a urea protein indicator.
The histopathological manifestations of urinary protein formation are mainly glomerular dilatation, capillary cell injury, and further plasma protein leakage from glomeruli, and renal ischemia causes the renal tubule reabsorption function to weaken, and when the urinary protein filtering amount exceeds the reabsorption amount, the urinary protein formation is caused. Therefore, urine protein generally reflects the degree of damage to the kidney function, and has the functions of primarily predicting kidney damage and accurately diagnosing symptoms of kidney damage. Therefore, the health condition of the pet can be reflected by detecting the urine protein content of the pet. Urine protein can be combined with various chemical reagents to generate a color change phenomenon, so that whether pets have diseases or not can be preliminarily judged through the color change phenomenon reaction.
The invention selects biuret and bromophenol blue as urine protein indicators. The biuret reaction is characteristic of peptides and proteins, compounds having two or more peptide bonds react with Cu under alkaline conditions2+The reaction produces a compound with red-purple color, and the color of the solution changes from basket to purple. The protein indicator used in the invention is applied to the cat litter preparation process by utilizing the color reaction, and can predict the health condition of the pet by judging whether the color of the cat litter changes in the using process, thereby playing roles in warning and preventing. At the same time. In order to improve the dip dyeing effect of the protein indicator, the pore silica gel obtained in the market is modified, so that the hydrophilic performance of the pore silica gel is improved, and the protein indicator can be efficiently dip-dyed on silica gel particles.
The cat litter bactericide selects the hexamethylene biguanide hydrochloride as the cat litter bactericide, can effectively sterilize and bacteriostasis, and compared with other solvent type bactericides, the cat litter bactericide is matched with silica gel particles, does not generate strong pungent smell, has good compatibility, low toxicity to pets, can be used for a long time, does not contain heavy metal and organic halogen, and does not cause adverse effect on the environment.
In conclusion, the beneficial effects of the invention are as follows: according to the invention, the biuret reaction is applied to the preparation process of the cat litter, and the silica gel substrate material and the biuret reagent are scientifically proportioned, so that the color reaction can be generated on abnormal urine protein, and the color reaction is not generated on normal urine, therefore, the health condition of pets can be preliminarily reflected, and the pets with abnormal urine can be diagnosed and examined in time, and an early warning effect is achieved. The added diatomite and the added active carbon are strong adsorptive porous substances, can strongly adsorb water and peculiar smell, and purify air. The invention also modifies silica gel, effectively increases the hydrophilicity of the silica gel, enhances the dip dyeing of the silica gel and biuret reagent, and ensures that the color reaction is obviously and easily distinguished. Meanwhile, the novel antibacterial agent is added, so that the cat litter has an obvious antibacterial effect and good persistence, and does not need to be replaced frequently. The invention has simple preparation process and low cost, and is suitable for popularization and use.
Drawings
FIG. 1 is a standard colorimetric card prepared by using biuret reagent as protein indicator according to the present invention.
Detailed Description
The technical solution of the present invention is further described below with reference to specific embodiments, but is not limited thereto.
Example 1
The cat litter with the pet urine protein early warning function is prepared from the following raw materials in parts by weight: 60 parts of modified fine-pore silica gel, 5 parts of diatomite, 5 parts of activated carbon, 100 parts of urine protein indicator and 0.1 part of antibacterial agent; the urine protein indicator is a biuret reagent.
The biuret reagent is prepared by the following method: weighing 1.5g of copper sulfate and 6.0g of potassium sodium tartrate, dissolving the copper sulfate and the potassium sodium tartrate in 500mL of distilled water, adding 300mL of 10% sodium hydroxide solution into the solution under stirring, and diluting the solution to 1L by using water;
the modified fine-pore silica gel is prepared by the following method:
(1) soaking the silica gel particles in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, and drying in a vacuum drying oven at 35-40 ℃ for 30min for later use;
(2) and (2) soaking the silica gel particles obtained in the step (1) in ethyl acetate for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and curing at 37 ℃ for 30min to obtain the modified fine-pore silica gel particles.
The antibacterial agent is polyhexamethylene biguanide hydrochloride. Polyhexamethylene biguanide hydrochloride, also known as PHMB, is available from Shanghai high-polymer biosciences, Inc. and has an active substance content of 20%.
A preparation method of cat litter with a pet urine protein early warning function comprises the following steps:
(1) pretreatment: adding 20ml of 0.2mol/L Na2HPO4 solution into 1000m1 of 0.1mol/L citric acid solution, and adjusting the pH to 2.1 to prepare a buffer solution; soaking the fine-pore silica gel in hydrochloric acid solution for 30min, taking out, washing with distilled water for 3-5 times, soaking in the obtained buffer solution for 30min, taking out, washing with distilled water for 3-5 times, and air drying;
(2) preparing modified fine-pore silica gel: soaking the silica gel particles obtained in the step (1) in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, placing in a vacuum drying oven for drying at 35-40 ℃ for 30min, soaking the silica gel particles in an ethyl acetate solution for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and then curing at 37 ℃ for 30min to obtain modified fine-pore silica gel particles;
(3) preparing a urine protein indicator: weighing 1.5g of copper sulfate and 6.0g of potassium sodium tartrate, dissolving the copper sulfate and the potassium sodium tartrate in 500mL of distilled water, adding 300mL of 10% sodium hydroxide solution into the solution under stirring, and diluting the solution to 1L by using water;
(4) weighing modified pore silica gel and biuret urine protein indicator according to a ratio, soaking the modified pore silica gel in biuret urine protein indicator solution, stirring, heating and stirring at 50 ℃ for 2-5h, filtering to separate out silica gel particles, and placing the silica gel particles in a vacuum drying oven for vacuum drying to obtain a primary finished product A1;
(5) mixing the diatomite, the activated carbon and the primary finished product A1 obtained in the step (4) according to the weight, and then placing the mixture into a stirrer for homogenization to obtain mixed particles B1;
(6) dissolving the antibacterial agent in 50-100 times of distilled water by weight to obtain an antibacterial agent solution; placing the mixed particles B1 obtained in the step (5) in a rotating disc, uniformly spraying an antibacterial agent solution on the rotating disc, placing the particles in a vacuum drying oven for drying after spraying is finished until the water content is 4-8%, and then packaging to obtain the finished cat litter C1;
the mass fraction of the ethyl acetate solution in the step (2) is 60-80%.
In the step (3), the temperature of the vacuum drying oven is 100 ℃, and the drying time is 12 hours.
When the cat litter with the pet urine protein early warning function is used, a color comparison card can be manufactured for convenience of use so as to quickly compare color changes. The color chart can be prepared by the following method:
(1) photographing the finished cat litter C1 and importing the photographed cat litter into a computer; taking urine protein overproof cat urine and normal cat urine to respectively soak finished cat litter C1, taking a picture of a color change result, guiding the picture into a computer, carrying out color difference inspection on the picture in the computer, and analyzing to obtain corresponding RGB values; manufacturing a color comparison card according to the corresponding RGB value;
the method for detecting the chromatic aberration comprises the following steps:
(1) generating an electronic Lauer color comparison card in a computer according to the RGB values of all colors in the Lauer color comparison card;
(2) placing the cat litter to be photographed and the real object Lauer colorimetric card together, photographing in the same environment, and then importing the photographed cat litter and the real object Lauer colorimetric card into a computer; and then verifying whether the RGB value of the real world Lauer colorimetric card is consistent with the RGB value of the standard Lauer colorimetric card of the electronic edition or not, and if so, indicating that the RGB value of the photo of the shot cat litter is not different.
Example 2
The cat litter with the pet urine protein early warning function is prepared from the following raw materials in parts by weight: 80 parts of modified fine-pore silica gel, 8 parts of diatomite, 8 parts of activated carbon, 130 parts of urine protein indicator and 0.3 part of antibacterial agent; the urine protein indicator is a biuret reagent.
The biuret reagent is prepared by the following method: weighing 1.5g of copper sulfate and 6.0g of potassium sodium tartrate, dissolving the copper sulfate and the potassium sodium tartrate in 500mL of distilled water, adding 300mL of 10% sodium hydroxide solution into the solution under stirring, and diluting the solution to 1L by using water;
the modified fine-pore silica gel is prepared by the following method:
(1) soaking the silica gel particles in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, and drying in a vacuum drying oven at 35-40 ℃ for 30min for later use;
(2) and (2) soaking the silica gel particles obtained in the step (1) in ethyl acetate for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and curing at 37 ℃ for 30min to obtain the modified fine-pore silica gel particles.
The antibacterial agent is polyhexamethylene biguanide hydrochloride. Polyhexamethylene biguanide hydrochloride, also known as PHMB, is available from Shanghai high-polymer biosciences, Inc. and has an active substance content of 20%.
A preparation method of cat litter with a pet urine protein early warning function comprises the following steps:
(1) pretreatment: adding 20ml of 0.2mol/L Na2HPO4 solution into 1000m1 of 0.1mol/L citric acid solution, and adjusting the pH to 2.1 to prepare a buffer solution; soaking the fine-pore silica gel in hydrochloric acid solution for 30min, taking out, washing with distilled water for 3-5 times, soaking in the obtained buffer solution for 30min, taking out, washing with distilled water for 3-5 times, and air drying;
(2) preparing modified fine-pore silica gel: soaking the silica gel particles obtained in the step (1) in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, placing in a vacuum drying oven for drying at 35-40 ℃ for 30min, soaking the silica gel particles in an ethyl acetate solution for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and then curing at 37 ℃ for 30min to obtain modified fine-pore silica gel particles;
(3) preparing a urine protein indicator: weighing 1.5g of copper sulfate and 6.0g of potassium sodium tartrate, dissolving the copper sulfate and the potassium sodium tartrate in 500mL of distilled water, adding 300mL of 10% sodium hydroxide solution into the solution under stirring, and diluting the solution to 1L by using water;
(4) weighing modified pore silica gel and biuret urine protein indicator according to a ratio, soaking the modified pore silica gel in biuret urine protein indicator solution, stirring, heating and stirring at 50 ℃ for 2-5h, filtering to separate out silica gel particles, and placing the silica gel particles in a vacuum drying oven for vacuum drying to obtain a primary finished product A1;
(5) mixing the diatomite, the activated carbon and the primary finished product A1 obtained in the step (4) according to the weight, and then placing the mixture into a stirrer for homogenization to obtain mixed particles B1;
(6) dissolving the antibacterial agent in 50-100 times of distilled water by weight to obtain an antibacterial agent solution; placing the mixed particles B1 obtained in the step (5) in a rotating disc, uniformly spraying an antibacterial agent solution on the rotating disc, placing the particles in a vacuum drying oven for drying after spraying is finished until the water content is 4-8%, and then packaging to obtain the finished cat litter C1;
the mass fraction of the ethyl acetate solution in the step (2) is 60-80%.
In the step (3), the temperature of the vacuum drying oven is 100 ℃, and the drying time is 12 hours.
When the cat litter with the pet urine protein early warning function is used, a color comparison card can be manufactured for convenience of use so as to quickly compare color changes. The color chart can be prepared by the following method:
(1) photographing the finished cat litter C1 and importing the photographed cat litter into a computer; taking urine protein overproof cat urine and normal cat urine to respectively soak finished cat litter C1, taking a picture of a color change result, guiding the picture into a computer, carrying out color difference inspection on the picture in the computer, and analyzing to obtain corresponding RGB values; manufacturing a color comparison card according to the corresponding RGB value;
the method for detecting the chromatic aberration comprises the following steps:
(1) generating an electronic Lauer color comparison card in a computer according to the RGB values of all colors in the Lauer color comparison card;
(2) placing the cat litter to be photographed and the real object Lauer colorimetric card together, photographing in the same environment, and then importing the photographed cat litter and the real object Lauer colorimetric card into a computer; and then verifying whether the RGB value of the real world Lauer colorimetric card is consistent with the RGB value of the standard Lauer colorimetric card of the electronic edition or not, and if so, indicating that the RGB value of the photo of the shot cat litter is not different.
Example 3
The cat litter with the pet urine protein early warning function is prepared from the following raw materials in parts by weight: 100 parts of modified fine-pore silica gel, 10 parts of diatomite, 10 parts of activated carbon, 150 parts of urine protein indicator and 0.5 part of antibacterial agent; the urine protein indicator is a biuret reagent.
The biuret reagent is prepared by the following method: weighing 1.5g of copper sulfate and 6.0g of potassium sodium tartrate, dissolving the copper sulfate and the potassium sodium tartrate in 500mL of distilled water, adding 300mL of 10% sodium hydroxide solution into the solution under stirring, and diluting the solution to 1L by using water;
the modified fine-pore silica gel is prepared by the following method:
(1) soaking the silica gel particles in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, and drying in a vacuum drying oven at 35-40 ℃ for 30min for later use;
(2) and (2) soaking the silica gel particles obtained in the step (1) in ethyl acetate for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and curing at 37 ℃ for 30min to obtain the modified fine-pore silica gel particles.
The antibacterial agent is polyhexamethylene biguanide hydrochloride. Polyhexamethylene biguanide hydrochloride, also known as PHMB, is available from Shanghai high-polymer biosciences, Inc. and has an active substance content of 20%.
A preparation method of cat litter with a pet urine protein early warning function comprises the following steps:
(1) pretreatment: adding 20ml of 0.2mol/L Na2HPO4 solution into 1000m1 of 0.1mol/L citric acid solution, and adjusting the pH to 2.1 to prepare a buffer solution; soaking the fine-pore silica gel in hydrochloric acid solution for 30min, taking out, washing with distilled water for 3-5 times, soaking in the obtained buffer solution for 30min, taking out, washing with distilled water for 3-5 times, and air drying;
(2) preparing modified fine-pore silica gel: soaking the silica gel particles obtained in the step (1) in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, placing in a vacuum drying oven for drying at 35-40 ℃ for 30min, soaking the silica gel particles in an ethyl acetate solution for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and then curing at 37 ℃ for 30min to obtain modified fine-pore silica gel particles;
(3) preparing a urine protein indicator: weighing 1.5g of copper sulfate and 6.0g of potassium sodium tartrate, dissolving the copper sulfate and the potassium sodium tartrate in 500mL of distilled water, adding 300mL of 10% sodium hydroxide solution into the solution under stirring, and diluting the solution to 1L by using water;
(4) weighing modified pore silica gel and biuret urine protein indicator according to a ratio, soaking the modified pore silica gel in biuret urine protein indicator solution, stirring, heating and stirring at 50 ℃ for 2-5h, filtering to separate out silica gel particles, and placing the silica gel particles in a vacuum drying oven for vacuum drying to obtain a primary finished product A1;
(5) mixing the diatomite, the activated carbon and the primary finished product A1 obtained in the step (4) according to the weight, and then placing the mixture into a stirrer for homogenization to obtain mixed particles B1;
(6) dissolving the antibacterial agent in 50-100 times of distilled water by weight to obtain an antibacterial agent solution; placing the mixed particles B1 obtained in the step (5) in a rotating disc, uniformly spraying an antibacterial agent solution on the rotating disc, placing the particles in a vacuum drying oven for drying after spraying is finished until the water content is 4-8%, and then packaging to obtain the finished cat litter C1;
the mass fraction of the ethyl acetate solution in the step (2) is 60-80%.
In the step (3), the temperature of the vacuum drying oven is 100 ℃, and the drying time is 12 hours.
When the cat litter with the pet urine protein early warning function is used, a color comparison card can be manufactured for convenience of use so as to quickly compare color changes. The color chart can be prepared by the following method:
(1) photographing the finished cat litter C1 and importing the photographed cat litter into a computer; taking urine protein overproof cat urine and normal cat urine to respectively soak finished cat litter C1, taking a picture of a color change result, guiding the picture into a computer, carrying out color difference inspection on the picture in the computer, and analyzing to obtain corresponding RGB values; manufacturing a color comparison card according to the corresponding RGB value;
the method for detecting the chromatic aberration comprises the following steps:
(1) generating an electronic Lauer color comparison card in a computer according to the RGB values of all colors in the Lauer color comparison card;
(2) placing the cat litter to be photographed and the real object Lauer colorimetric card together, photographing in the same environment, and then importing the photographed cat litter and the real object Lauer colorimetric card into a computer; and then verifying whether the RGB value of the real object Lauer colorimetric card is consistent with the RGB value of the standard Lauer colorimetric card of the electronic edition, and if so, showing that the RGB value of the photo of the shot cat litter is not different.
Example 4
The cat litter with the pet urine protein early warning function is prepared from the following raw materials in parts by weight: 60 parts of modified fine-pore silica gel, 5 parts of diatomite, 5 parts of activated carbon, 100 parts of urine protein indicator and 0.1 part of antibacterial agent; the urine protein indicator is a bromophenol blue reagent.
The bromophenol blue reagent is prepared by the following method: 0.67g of bromophenol blue is dissolved in 10ml of 0.1mol/L NaOH solution and distilled water is added to 1000 ml.
The modified fine-pore silica gel is prepared by the following method:
(1) soaking the silica gel particles in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, and drying in a vacuum drying oven at 35-40 ℃ for 30min for later use;
(2) and (2) soaking the silica gel particles obtained in the step (1) in ethyl acetate for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and curing at 37 ℃ for 30min to obtain the modified fine-pore silica gel particles.
The antibacterial agent is polyhexamethylene biguanide hydrochloride. Polyhexamethylene biguanide hydrochloride, also known as PHMB, is available from Shanghai high-polymer biosciences, Inc. and has an active substance content of 20%.
A preparation method of cat litter with a pet urine protein early warning function comprises the following steps:
(1) pretreatment: adding 20ml of 0.2mol/L Na2HPO4 solution into 1000m1 of 0.1mol/L citric acid solution, and adjusting the pH to 2.1 to prepare a buffer solution; soaking the fine-pore silica gel in hydrochloric acid solution for 30min, taking out, washing with distilled water for 3-5 times, soaking in the obtained buffer solution for 30min, taking out, washing with distilled water for 3-5 times, and air drying;
(2) preparing modified fine-pore silica gel: soaking the silica gel particles obtained in the step (1) in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, placing in a vacuum drying oven for drying at 35-40 ℃ for 30min, soaking the silica gel particles in an ethyl acetate solution for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and then curing at 37 ℃ for 30min to obtain modified fine-pore silica gel particles;
(3) preparing a urine protein indicator: weighing 0.67g of bromophenol blue, dissolving in 10ml of 0.1mol/L NaOH solution, and adding distilled water to 1000 ml;
(4) weighing modified porous silica gel and bromophenol blue urine protein indicator according to a ratio, soaking the modified porous silica gel in bromophenol blue urine protein indicator solution, stirring, heating and stirring at 50 ℃ for 2-5h, filtering to separate out silica gel particles, and placing in a vacuum drying oven for vacuum drying to obtain a primary finished product A2;
(5) mixing the diatomite, the activated carbon and the primary finished product A2 obtained in the step (4) according to the weight, and then placing the mixture into a stirrer for homogenization to obtain mixed particles B2;
(6) dissolving the antibacterial agent in 50-100 times of distilled water by weight to obtain an antibacterial agent solution; and (3) placing the mixed particles B2 obtained in the step (A) in a rotating disc, uniformly spraying an antibacterial agent solution on the mixed particles B2, placing the particles in a vacuum drying oven to be dried after the spraying is finished until the water content is 4-8%, and then packaging to obtain the finished cat litter C2.
The mass fraction of the ethyl acetate solution in the step (2) is 60-80%.
In the step (3), the temperature of the vacuum drying oven is 100 ℃, and the drying time is 12 hours.
When the cat litter with the pet urine protein early warning function is used, a color comparison card can be manufactured for convenience of use so as to quickly compare color changes. The color chart can be prepared by the following method:
(1) photographing the finished cat litter C2 and importing the photographed cat litter into a computer; taking urine protein overproof cat urine and normal cat urine to respectively soak finished cat litter C2, taking a picture of a color change result, guiding the picture into a computer, carrying out color difference inspection on the picture in the computer, and analyzing to obtain corresponding RGB values; and manufacturing a color comparison card according to the corresponding RGB value.
The method for detecting the chromatic aberration comprises the following steps:
(1) generating an electronic Lauer color comparison card in a computer according to the RGB values of all colors in the Lauer color comparison card;
(2) placing the cat litter to be photographed and the real object Lauer colorimetric card together, photographing in the same environment, and then importing the photographed cat litter and the real object Lauer colorimetric card into a computer; and then verifying whether the RGB value of the real object Lauer colorimetric card is consistent with the RGB value of the standard Lauer colorimetric card of the electronic edition, and if so, showing that the RGB value of the photo of the shot cat litter is not different.
Example 5
The cat litter with the pet urine protein early warning function is prepared from the following raw materials in parts by weight: 80 parts of modified fine-pore silica gel, 8 parts of diatomite, 8 parts of activated carbon, 120 parts of urine protein indicator and 0.3 part of antibacterial agent; the urine protein indicator is a bromophenol blue reagent.
The bromophenol blue reagent is prepared by the following method: 0.67g of bromophenol blue is dissolved in 10ml of 0.1mol/L NaOH solution and distilled water is added to 1000 ml.
The modified fine-pore silica gel is prepared by the following method:
(1) soaking the silica gel particles in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, and drying in a vacuum drying oven at 35-40 ℃ for 30min for later use;
(2) and (2) soaking the silica gel particles obtained in the step (1) in ethyl acetate for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and curing at 37 ℃ for 30min to obtain the modified fine-pore silica gel particles.
The antibacterial agent is polyhexamethylene biguanide hydrochloride. Polyhexamethylene biguanide hydrochloride, also known as PHMB, is available from Shanghai high-polymer biosciences, Inc. and has an active substance content of 20%.
A preparation method of cat litter with a pet urine protein early warning function comprises the following steps:
(1) pretreatment: adding 20ml of 0.2mol/L Na2HPO4 solution into 1000ml of 0.1mol/L citric acid solution, and adjusting the pH to 2.1 to prepare a buffer solution; soaking the fine-pore silica gel in hydrochloric acid solution for 30min, taking out, washing with distilled water for 3-5 times, soaking in the obtained buffer solution for 30min, taking out, washing with distilled water for 3-5 times, and air drying;
(2) preparing modified fine-pore silica gel: soaking the silica gel particles obtained in the step (1) in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, placing in a vacuum drying oven for drying at 35-40 ℃ for 30min, soaking the silica gel particles in an ethyl acetate solution for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and then curing at 37 ℃ for 30min to obtain modified fine-pore silica gel particles;
(3) preparing a urine protein indicator: weighing 0.67g of bromophenol blue, dissolving in 10ml of 0.1mol/L NaOH solution, and adding distilled water to 1000 ml;
(4) weighing modified porous silica gel and bromophenol blue urine protein indicator according to a ratio, soaking the modified porous silica gel in bromophenol blue urine protein indicator solution, stirring, heating and stirring at 50 ℃ for 2-5h, filtering to separate out silica gel particles, and placing in a vacuum drying oven for vacuum drying to obtain a primary finished product A2;
(5) mixing the diatomite, the activated carbon and the primary finished product A2 obtained in the step (4) according to the weight, and then placing the mixture into a stirrer for homogenization to obtain mixed particles B2;
(6) dissolving the antibacterial agent in 50-100 times of distilled water by weight to obtain an antibacterial agent solution; and (3) placing the mixed particles B2 obtained in the step (A) in a rotating disc, uniformly spraying an antibacterial agent solution on the mixed particles B2, placing the particles in a vacuum drying oven to be dried after the spraying is finished until the water content is 4-8%, and then packaging to obtain the finished cat litter C2.
The mass fraction of the ethyl acetate solution in the step (2) is 60-80%.
In the step (3), the temperature of the vacuum drying oven is 100 ℃, and the drying time is 12 hours.
When the cat litter with the pet urine protein early warning function is used, a color comparison card can be manufactured for convenience of use so as to quickly compare color changes. The color chart can be prepared by the following method:
(2) photographing the finished cat litter C2 and importing the photographed cat litter into a computer; taking urine protein overproof cat urine and normal cat urine to respectively soak finished cat litter C2, taking a picture of a color change result, guiding the picture into a computer, carrying out color difference inspection on the picture in the computer, and analyzing to obtain corresponding RGB values; and manufacturing a color comparison card according to the corresponding RGB value.
The method for detecting the chromatic aberration comprises the following steps:
(1) generating an electronic Lauer color comparison card in a computer according to the RGB values of all colors in the Lauer color comparison card;
(2) placing the cat litter to be photographed and the real object Lauer colorimetric card together, photographing in the same environment, and then importing the photographed cat litter and the real object Lauer colorimetric card into a computer; and then verifying whether the RGB value of the real world Lauer colorimetric card is consistent with the RGB value of the standard Lauer colorimetric card of the electronic edition or not, and if so, indicating that the RGB value of the photo of the shot cat litter is not different.
Example 6
The cat litter with the pet urine protein early warning function is prepared from the following raw materials in parts by weight: 100 parts of modified fine-pore silica gel, 10 parts of diatomite, 10 parts of activated carbon, 150 parts of urine protein indicator and 0.5 part of antibacterial agent; the urine protein indicator is a bromophenol blue reagent.
The bromophenol blue reagent is prepared by the following method: 0.67g of bromophenol blue is dissolved in 10ml of 0.1mol/L NaOH solution and distilled water is added to 1000 ml.
The modified fine-pore silica gel is prepared by the following method:
(1) soaking the silica gel particles in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, and drying in a vacuum drying oven at 35-40 ℃ for 30min for later use;
(2) and (2) soaking the silica gel particles obtained in the step (1) in ethyl acetate for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and curing at 37 ℃ for 30min to obtain the modified fine-pore silica gel particles.
The antibacterial agent is polyhexamethylene biguanide hydrochloride. Polyhexamethylene biguanide hydrochloride, also known as PHMB, is available from Shanghai high-polymer biosciences, Inc. and has an active substance content of 20%.
A preparation method of cat litter with a pet urine protein early warning function comprises the following steps:
(1) pretreatment: adding 20ml of 0.2mol/L Na2HPO4 solution into 1000m1 of 0.1mol/L citric acid solution, and adjusting the pH to 2.1 to prepare a buffer solution; soaking the fine-pore silica gel in hydrochloric acid solution for 30min, taking out, washing with distilled water for 3-5 times, soaking in the obtained buffer solution for 30min, taking out, washing with distilled water for 3-5 times, and air drying;
(2) preparing modified fine-pore silica gel: soaking the silica gel particles obtained in the step (1) in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, placing in a vacuum drying oven for drying at 35-40 ℃ for 30min, soaking the silica gel particles in an ethyl acetate solution for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and then curing at 37 ℃ for 30min to obtain modified fine-pore silica gel particles;
(3) preparing a urine protein indicator: preparing a bromophenol blue reagent, namely weighing 0.67g of bromophenol blue, dissolving the bromophenol blue in 10ml of 0.1mol/L NaOH solution, and adding distilled water to 1000 ml;
(4) weighing modified porous silica gel and bromophenol blue urine protein indicator according to a ratio, soaking the modified porous silica gel in bromophenol blue urine protein indicator solution, stirring, heating and stirring at 50 ℃ for 2-5h, filtering to separate out silica gel particles, and placing in a vacuum drying oven for vacuum drying to obtain a primary finished product A2;
(5) mixing the diatomite, the activated carbon and the primary finished product A2 obtained in the step (4) according to the weight, and then placing the mixture into a stirrer for homogenization to obtain mixed particles B2;
(6) dissolving the antibacterial agent in 50-100 times of distilled water by weight to obtain an antibacterial agent solution; and (3) placing the mixed particles B2 obtained in the step (A) in a rotating disc, uniformly spraying an antibacterial agent solution on the mixed particles B2, placing the particles in a vacuum drying oven to be dried after the spraying is finished until the water content is 4-8%, and then packaging to obtain the finished cat litter C2.
The mass fraction of the ethyl acetate solution in the step (2) is 60-80%.
In the step (3), the temperature of the vacuum drying oven is 100 ℃, and the drying time is 12 hours.
When the cat litter with the pet urine protein early warning function is used, a color comparison card can be manufactured for convenience of use so as to quickly compare color changes. The color chart can be prepared by the following method:
(1) photographing the finished cat litter C2 and importing the photographed cat litter into a computer; taking urine protein overproof cat urine and normal cat urine to respectively soak finished cat litter C2, taking a picture of a color change result, guiding the picture into a computer, carrying out color difference inspection on the picture in the computer, and analyzing to obtain corresponding RGB values; and manufacturing a color comparison card according to the corresponding RGB value.
The method for detecting the chromatic aberration comprises the following steps:
(1) generating an electronic Lauer color comparison card in a computer according to the RGB values of all colors in the Lauer color comparison card;
(2) placing the cat litter to be photographed and the real object Lauer colorimetric card together, photographing in the same environment, and then importing the photographed cat litter and the real object Lauer colorimetric card into a computer; and then verifying whether the RGB value of the real object Lauer colorimetric card is consistent with the RGB value of the standard Lauer colorimetric card of the electronic edition, and if so, showing that the RGB value of the photo of the shot cat litter is not different.
Application test
Preparing a standard colorimetric card: normal cat urine protein values were 15mol/dL, and urine solutions containing 10 mol/dL, 15mol/dL, 20 mol/dL, and 25 mol/dL of urine were selected to prepare color charts as described in examples 1-6, wherein clear water was used as a blank control, and the color charts were prepared with a gradual color change. Wherein, the colorimetric card prepared by using the biuret reagent as the protein indicator is shown in figure 1. When the color changes to purple and is similar to the 15-20 grades, the abnormal urine protein is indicated. The vigilance should be improved. In the same way, a standard colorimetric card taking a bromophenol blue reagent as a protein indicator can be prepared.
The litters obtained in examples 1-6 of the present invention and commercially available ordinary silica gel litters were subjected to urine test experiments. Selecting 35 sick cats with abnormal renal function and urine protein higher than normal value, randomly dividing the sick cats into 7 groups, and respectively using S1-S6 and commercial common cat litters for the invention of examples 1-6 and S7; 35 cats with normal physiological indexes were selected and randomly divided into 7 groups, and commercially available cat litters were used in T1-T6 according to examples 1-6 and T7 of the present invention, respectively.
Statistical performance data, as shown in table 1 below:
TABLE 1 comparison of the Performance of cat litters of the invention with commercial litters
Compared with the commercially available common cat litter, the embodiment of the invention has high urine absorption speed, the shortest time can reach 13 seconds, and the commercially available cat litter needs more than 50 seconds; meanwhile, the time for dehydrating and hardening the excrement is only about 200 seconds, which is far less than 400 seconds of the cat litter sold on the market. And the capability of absorbing the peculiar smell is relatively better. Presumably, the silica gel has good hydrophilic modification effect, so that the protein indicator is fully impregnated in the silica gel, abnormal urine of urine protein has color reaction, and normal urine has no color reaction, thereby helping pet raising personnel preliminarily know the health condition of pets and playing a warning role.
It should be noted that the above-mentioned embodiments are only some of the preferred modes for implementing the invention, and not all of them. Obviously, all other embodiments obtained by persons of ordinary skill in the art based on the above-mentioned embodiments of the present invention without any creative effort shall fall within the protection scope of the present invention.
Claims (4)
1. The cat litter with the pet urine protein early warning function is characterized by being prepared from the following raw materials in parts by weight: 60-100 parts of modified fine-pore silica gel, 5-10 parts of diatomite, 5-10 parts of activated carbon, 100-150 parts of urine protein indicator and 0.1-0.5 part of antibacterial agent; the urine protein indicator is a bromophenol blue reagent or a biuret reagent;
the preparation method of the cat litter comprises the following steps:
(1) pretreatment: 0.2mol/L Na is added into 1000m1 of 0.1mol/L citric acid liquid2HPO4Adjusting the pH value of the solution to be 2.1 by 20ml to prepare a buffer solution; soaking the fine-pore silica gel in hydrochloric acid solution for 30min, taking out, washing with distilled water for 3-5 times, soaking in the obtained buffer solution for 30min, taking out, washing with distilled water for 3-5 times, and air drying;
(2) preparing modified fine-pore silica gel: soaking the silica gel particles obtained in the step (1) in an ethanol solution with the mass fraction of 60%, ultrasonically cleaning for 30min, cleaning with distilled water, placing in a vacuum drying oven for drying at 35-40 ℃ for 30min, soaking the silica gel particles in an ethyl acetate solution for 10-15min, taking out, soaking in a polycarbodiimide solution with the mass fraction of 15% for 2min, and then curing at 37 ℃ for 30min to obtain modified fine-pore silica gel particles;
(3) preparing a urine protein indicator: preparing a biuret reagent, weighing 1.5g of copper sulfate and 6.0g of potassium sodium tartrate, dissolving with 500mL of distilled water, adding 300mL of 10% sodium hydroxide solution under stirring, and diluting with water to 1L; preparing a bromophenol blue reagent, namely weighing 0.67g of bromophenol blue, dissolving the bromophenol blue in 10ml of 0.1mol/L NaOH solution, and adding distilled water to 1000 ml;
(4) weighing modified pore silica gel and biuret urine protein indicator according to a ratio, soaking the modified pore silica gel in biuret urine protein indicator solution, stirring, heating and stirring at 50 ℃ for 2-5h, filtering to separate out silica gel particles, and placing the silica gel particles in a vacuum drying oven for vacuum drying to obtain a primary finished product A1; weighing modified porous silica gel and bromophenol blue urine protein indicator according to a ratio, soaking the modified porous silica gel in bromophenol blue urine protein indicator solution, stirring, heating and stirring at 50 ℃ for 2-5h, filtering to separate out silica gel particles, and placing in a vacuum drying oven for vacuum drying to obtain a primary finished product A2;
(5) mixing the diatomite, the activated carbon and the primary finished product A1 obtained in the step (4) according to the weight, and then placing the mixture into a stirrer for homogenization to obtain mixed particles B1;
(6) dissolving the antibacterial agent in 50-100 times of distilled water by weight to obtain an antibacterial agent solution; placing the mixed particles B1 obtained in the step (5) in a rotating disc, uniformly spraying an antibacterial agent solution on the rotating disc, placing the particles in a vacuum drying oven for drying after spraying is finished until the water content is 4-8%, and then packaging to obtain the finished cat litter C1;
(7) mixing the diatomite, the activated carbon and the primary finished product A2 obtained in the step (4) according to the weight, and then placing the mixture into a stirrer for homogenization to obtain mixed particles B2;
(8) dissolving the antibacterial agent in 50-100 times of distilled water by weight to obtain an antibacterial agent solution; and (3) placing the mixed particles B2 obtained in the step (7) in a rotating disc, uniformly spraying an antibacterial agent solution on the rotating disc, placing the particles in a vacuum drying oven for drying after spraying is finished until the water content is 4-8%, and then packaging to obtain the finished cat litter C2.
2. The cat litter with pet urine protein warning function as claimed in claim 1, wherein the antibacterial agent is polyhexamethylene biguanide hydrochloride.
3. The cat litter with the pet urine protein early warning function as claimed in claim 1, wherein the mass fraction of the ethyl acetate solution in the step (2) is 60-80%.
4. The cat litter with the pet urine protein early warning function according to claim 1, wherein the temperature of the vacuum drying oven in the step (4) is 100 ℃ and the drying time is 12 hours.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811247499.0A CN109287499B (en) | 2018-10-25 | 2018-10-25 | Cat litter with pet urine protein early warning function and preparation method thereof |
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| CN201811247499.0A CN109287499B (en) | 2018-10-25 | 2018-10-25 | Cat litter with pet urine protein early warning function and preparation method thereof |
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| CN112790113A (en) * | 2021-01-15 | 2021-05-14 | 上海毛球日记宠物用品有限公司 | Water-absorbing color-changing cat litter convenient to clean and preparation method thereof |
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